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Protein

Vacuolar protein sorting-associated protein 21

Gene

VPS21

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Required for protein transport to the vacuole. Involved in two vesicle trafficking steps to the prevacuolar compartment (PVC), regulating the docking of endosomes and Golgi vesicles to the PVC by interacting with PEP7/VAC1 on the PVC membrane and promoting SNARE complex formation.2 Publications

Miscellaneous

Present with 8759 molecules/cell in log phase SD medium.1 Publication

Enzyme regulationi

Alternates between an inactive form bound to GDP and an active form bound to GTP. Activated by the guanine nucleotide-exchange factor (GEF) VPS9 and inactivated by GTPase-activating proteins (GAPs) GYP1 and GYP3.3 Publications

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi14 – 21GTP8
Nucleotide bindingi62 – 66GTP5
Nucleotide bindingi120 – 123GTP4

GO - Molecular functioni

  • GTPase activity Source: SGD
  • GTP binding Source: UniProtKB-KW

GO - Biological processi

  • endocytosis Source: SGD
  • Golgi to endosome transport Source: SGD
  • late endosome to vacuole transport via multivesicular body sorting pathway Source: SGD
  • multivesicular body assembly Source: SGD
  • protein localization to endosome Source: SGD
  • protein targeting to vacuole Source: SGD
  • reticulophagy Source: SGD
  • vacuole inheritance Source: SGD

Keywordsi

Biological processProtein transport, Transport
LigandGTP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciYEAST:G3O-33623-MONOMER
ReactomeiR-SCE-6798695 Neutrophil degranulation
R-SCE-8854214 TBC/RABGAPs
R-SCE-8873719 RAB geranylgeranylation
R-SCE-8876198 RAB GEFs exchange GTP for GDP on RABs

Names & Taxonomyi

Protein namesi
Recommended name:
Vacuolar protein sorting-associated protein 21
Alternative name(s):
GTP-binding protein YPT51
Vacuolar protein-targeting protein 12
Gene namesi
Name:VPS21
Synonyms:VPS12, VPT12, YPT21, YPT51
Ordered Locus Names:YOR089C
ORF Names:YOR3154C
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome XV

Organism-specific databases

EuPathDBiFungiDB:YOR089C
SGDiS000005615 VPS21

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Endosome, Membrane, Mitochondrion

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi21S → L or N: Locks VPS21 in its GDP-bound form by abolishing binding to GTP. 3 Publications1
Mutagenesisi66Q → L: Abolishes GTP hydrolysis. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001213251 – 210Vacuolar protein sorting-associated protein 21Add BLAST210

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei188PhosphoserineCombined sources1
Lipidationi208S-geranylgeranyl cysteineBy similarity1
Modified residuei210Cysteine methyl esterBy similarity1
Lipidationi210S-geranylgeranyl cysteineBy similarity1

Keywords - PTMi

Lipoprotein, Methylation, Phosphoprotein, Prenylation

Proteomic databases

MaxQBiP36017
PaxDbiP36017
PRIDEiP36017

PTM databases

iPTMnetiP36017

Interactioni

Subunit structurei

Interacts in its active GTP-bound form with PEP7/VAC1.2 Publications

Binary interactionsi

Show more details

Protein-protein interaction databases

BioGridi34487, 581 interactors
DIPiDIP-3801N
IntActiP36017, 41 interactors
MINTiP36017
STRINGi4932.YOR089C

Structurei

Secondary structure

1210
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi6 – 13Combined sources8
Helixi20 – 29Combined sources10
Beta strandi40 – 51Combined sources12
Beta strandi54 – 63Combined sources10
Helixi67 – 72Combined sources6
Helixi73 – 77Combined sources5
Beta strandi81 – 88Combined sources8
Helixi92 – 108Combined sources17
Beta strandi114 – 120Combined sources7
Helixi122 – 126Combined sources5
Helixi135 – 145Combined sources11
Beta strandi148 – 151Combined sources4
Turni154 – 156Combined sources3
Helixi160 – 168Combined sources9

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1EK0X-ray1.48A5-174[»]
ProteinModelPortaliP36017
SMRiP36017
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP36017

Family & Domainsi

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi36 – 44Effector regionSequence analysis9

Sequence similaritiesi

Belongs to the small GTPase superfamily. Rab family.Curated

Phylogenomic databases

GeneTreeiENSGT00760000119101
HOGENOMiHOG000233968
InParanoidiP36017
KOiK07889
OMAiLIKAKHW
OrthoDBiEOG092C5MDH

Family and domain databases

InterProiView protein in InterPro
IPR027417 P-loop_NTPase
IPR005225 Small_GTP-bd_dom
IPR001806 Small_GTPase
PfamiView protein in Pfam
PF00071 Ras, 1 hit
SUPFAMiSSF52540 SSF52540, 1 hit
TIGRFAMsiTIGR00231 small_GTP, 1 hit
PROSITEiView protein in PROSITE
PS51419 RAB, 1 hit

Sequencei

Sequence statusi: Complete.

P36017-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MNTSVTSIKL VLLGEAAVGK SSIVLRFVSN DFAENKEPTI GAAFLTQRVT
60 70 80 90 100
INEHTVKFEI WDTAGQERFA SLAPMYYRNA QAALVVYDVT KPQSFIKARH
110 120 130 140 150
WVKELHEQAS KDIIIALVGN KIDMLQEGGE RKVAREEGEK LAEEKGLLFF
160 170 180 190 200
ETSAKTGENV NDVFLGIGEK IPLKTAEEQN SASNERESNN QRVDLNAAND
210
GTSANSACSC
Length:210
Mass (Da):23,081
Last modified:June 1, 1994 - v1
Checksum:i34D7DA0090C33F09
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X76173 Genomic DNA Translation: CAA53769.1
Z29338 Genomic DNA Translation: CAA82543.1
X94335 Genomic DNA Translation: CAA64010.1
Z74997 Genomic DNA Translation: CAA99285.1
BK006948 Genomic DNA Translation: DAA10866.1
PIRiS43399
RefSeqiNP_014732.1, NM_001183508.1

Genome annotation databases

EnsemblFungiiYOR089C; YOR089C; YOR089C
GeneIDi854256
KEGGisce:YOR089C

Similar proteinsi

Entry informationi

Entry nameiVPS21_YEAST
AccessioniPrimary (citable) accession number: P36017
Secondary accession number(s): D6W2F0
Entry historyiIntegrated into UniProtKB/Swiss-Prot: June 1, 1994
Last sequence update: June 1, 1994
Last modified: May 23, 2018
This is version 174 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

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