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Protein

Heat shock protein 70 homolog LHS1

Gene

LHS1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Chaperone required for protein translocation and folding in the endoplasmic reticulum.1 Publication

Catalytic activityi

ATP + H2O = ADP + phosphate.

GO - Molecular functioni

  1. adenyl-nucleotide exchange factor activity Source: SGD
  2. ATPase activity Source: UniProtKB-EC
  3. ATP binding Source: SGD
  4. unfolded protein binding Source: SGD

GO - Biological processi

  1. posttranslational protein targeting to membrane, translocation Source: SGD
  2. regulation of catalytic activity Source: GOC
  3. response to unfolded protein Source: SGD
Complete GO annotation...

Keywords - Molecular functioni

Chaperone, Hydrolase

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciYEAST:G3O-31869-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Heat shock protein 70 homolog LHS1 (EC:3.6.1.3)
Gene namesi
Name:LHS1
Ordered Locus Names:YKL073W
ORF Names:YKL355
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
ProteomesiUP000002311: Chromosome XI

Organism-specific databases

CYGDiYKL073w.
SGDiS000001556. LHS1.

Subcellular locationi

GO - Cellular componenti

  1. endoplasmic reticulum lumen Source: SGD
Complete GO annotation...

Keywords - Cellular componenti

Endoplasmic reticulum

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi239 – 2391G → D in allele LHS1-1; constitutive ATPase activity. 1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 2020Sequence AnalysisAdd
BLAST
Chaini21 – 881861Heat shock protein 70 homolog LHS1PRO_0000013556Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi128 – 1281N-linked (GlcNAc...)Sequence Analysis
Glycosylationi458 – 4581N-linked (GlcNAc...)Sequence Analysis
Glycosylationi474 – 4741N-linked (GlcNAc...)Sequence Analysis
Glycosylationi481 – 4811N-linked (GlcNAc...)Sequence Analysis
Glycosylationi489 – 4891N-linked (GlcNAc...)Sequence Analysis
Glycosylationi527 – 5271N-linked (GlcNAc...)Sequence Analysis
Glycosylationi844 – 8441N-linked (GlcNAc...)Sequence Analysis

Post-translational modificationi

N-glycosylated.

Keywords - PTMi

Glycoprotein

Proteomic databases

MaxQBiP36016.
PaxDbiP36016.
PeptideAtlasiP36016.
PRIDEiP36016.

Expressioni

Inductioni

By the unfolded protein response (UPR).1 Publication

Gene expression databases

GenevestigatoriP36016.

Interactioni

Subunit structurei

Interacts with the heat shock protein 70 (HSP70) KAR2, and this stimulates nucleotide exchange on KAR2. KAR2 in turn acts to stimulate the ATPase activity of LHS1.1 Publication

Protein-protein interaction databases

BioGridi34058. 139 interactions.
DIPiDIP-6520N.
IntActiP36016. 3 interactions.
MINTiMINT-684320.
STRINGi4932.YKL073W.

Structurei

3D structure databases

ProteinModelPortaliP36016.
SMRiP36016. Positions 20-671.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi878 – 8814Prevents secretion from ERPROSITE-ProRule annotation

Sequence similaritiesi

Belongs to the heat shock protein 70 family.Curated

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiCOG0443.
GeneTreeiENSGT00780000122466.
HOGENOMiHOG000111144.
InParanoidiP36016.
KOiK09486.
OMAiIKFDLGF.
OrthoDBiEOG7DC2CX.

Family and domain databases

Gene3Di1.20.1270.10. 1 hit.
InterProiIPR018181. Heat_shock_70_CS.
IPR029048. HSP70_C.
IPR013126. Hsp_70_fam.
[Graphical view]
PfamiPF00012. HSP70. 1 hit.
[Graphical view]
PRINTSiPR00301. HEATSHOCK70.
PROSITEiPS00014. ER_TARGET. 1 hit.
PS00329. HSP70_2. 1 hit.
PS01036. HSP70_3. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P36016-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MRNVLRLLFL TAFVAIGSLA AVLGVDYGQQ NIKAIVVSPQ APLELVLTPE
60 70 80 90 100
AKRKEISGLS IKRLPGYGKD DPNGIERIYG SAVGSLATRF PQNTLLHLKP
110 120 130 140 150
LLGKSLEDET TVTLYSKQHP GLEMVSTNRS TIAFLVDNVE YPLEELVAMN
160 170 180 190 200
VQEIANRANS LLKDRDARTE DFVNKMSFTI PDFFDQHQRK ALLDASSITT
210 220 230 240 250
GIEETYLVSE GMSVAVNFVL KQRQFPPGEQ QHYIVYDMGS GSIKASMFSI
260 270 280 290 300
LQPEDTTQPV TIEFEGYGYN PHLGGAKFTM DIGSLIENKF LETHPAIRTD
310 320 330 340 350
ELHANPKALA KINQAAEKAK LILSANSEAS INIESLINDI DFRTSITRQE
360 370 380 390 400
FEEFIADSLL DIVKPINDAV TKQFGGYGTN LPEINGVILA GGSSRIPIVQ
410 420 430 440 450
DQLIKLVSEE KVLRNVNADE SAVNGVVMRG IKLSNSFKTK PLNVVDRSVN
460 470 480 490 500
TYSFKLSNES ELYDVFTRGS AYPNKTSILT NTTDSIPNNF TIDLFENGKL
510 520 530 540 550
FETITVNSGA IKNSYSSDKC SSGVAYNITF DLSSDRLFSI QEVNCICQSE
560 570 580 590 600
NDIGNSKQIK NKGSRLAFTS EDVEIKRLSP SERSRLHEHI KLLDKQDKER
610 620 630 640 650
FQFQENLNVL ESNLYDARNL LMDDEVMQNG PKSQVEELSE MVKVYLDWLE
660 670 680 690 700
DASFDTDPED IVSRIREIGI LKKKIELYMD SAKEPLNSQQ FKGMLEEGHK
710 720 730 740 750
LLQAIETHKN TVEEFLSQFE TEFADTIDNV REEFKKIKQP AYVSKALSTW
760 770 780 790 800
EETLTSFKNS ISEIEKFLAK NLFGEDLREH LFEIKLQFDM YRTKLEEKLR
810 820 830 840 850
LIKSGDESRL NEIKKLHLRN FRLQKRKEEK LKRKLEQEKS RNNNETESTV
860 870 880
INSADDKTTI VNDKTTESNP SSEEDILHDE L
Length:881
Mass (Da):99,572
Last modified:June 1, 1994 - v1
Checksum:iACED092CA3A34785
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X75780 Genomic DNA. Translation: CAA53401.1.
Z28073 Genomic DNA. Translation: CAA81910.1.
BK006944 Genomic DNA. Translation: DAA09083.1.
PIRiS37895.
RefSeqiNP_012850.1. NM_001179639.1.

Genome annotation databases

EnsemblFungiiYKL073W; YKL073W; YKL073W.
GeneIDi853789.
KEGGisce:YKL073W.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X75780 Genomic DNA. Translation: CAA53401.1.
Z28073 Genomic DNA. Translation: CAA81910.1.
BK006944 Genomic DNA. Translation: DAA09083.1.
PIRiS37895.
RefSeqiNP_012850.1. NM_001179639.1.

3D structure databases

ProteinModelPortaliP36016.
SMRiP36016. Positions 20-671.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi34058. 139 interactions.
DIPiDIP-6520N.
IntActiP36016. 3 interactions.
MINTiMINT-684320.
STRINGi4932.YKL073W.

Proteomic databases

MaxQBiP36016.
PaxDbiP36016.
PeptideAtlasiP36016.
PRIDEiP36016.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYKL073W; YKL073W; YKL073W.
GeneIDi853789.
KEGGisce:YKL073W.

Organism-specific databases

CYGDiYKL073w.
SGDiS000001556. LHS1.

Phylogenomic databases

eggNOGiCOG0443.
GeneTreeiENSGT00780000122466.
HOGENOMiHOG000111144.
InParanoidiP36016.
KOiK09486.
OMAiIKFDLGF.
OrthoDBiEOG7DC2CX.

Enzyme and pathway databases

BioCyciYEAST:G3O-31869-MONOMER.

Miscellaneous databases

NextBioi974918.

Gene expression databases

GenevestigatoriP36016.

Family and domain databases

Gene3Di1.20.1270.10. 1 hit.
InterProiIPR018181. Heat_shock_70_CS.
IPR029048. HSP70_C.
IPR013126. Hsp_70_fam.
[Graphical view]
PfamiPF00012. HSP70. 1 hit.
[Graphical view]
PRINTSiPR00301. HEATSHOCK70.
PROSITEiPS00014. ER_TARGET. 1 hit.
PS00329. HSP70_2. 1 hit.
PS01036. HSP70_3. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Sequence of a 20.7 kb region of yeast chromosome XI includes the NUP100 gene, an open reading frame (ORF) possibly representing a nucleoside diphosphate kinase gene, tRNAs for His, Val and Trp in addition to seven ORFs with weak or no significant similarity to known proteins."
    Rasmussen S.W.
    Yeast 10:S69-S74(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  2. "Complete DNA sequence of yeast chromosome XI."
    Dujon B., Alexandraki D., Andre B., Ansorge W., Baladron V., Ballesta J.P.G., Banrevi A., Bolle P.-A., Bolotin-Fukuhara M., Bossier P., Bou G., Boyer J., Buitrago M.J., Cheret G., Colleaux L., Daignan-Fornier B., del Rey F., Dion C.
    , Domdey H., Duesterhoeft A., Duesterhus S., Entian K.-D., Erfle H., Esteban P.F., Feldmann H., Fernandes L., Fobo G.M., Fritz C., Fukuhara H., Gabel C., Gaillon L., Garcia-Cantalejo J.M., Garcia-Ramirez J.J., Gent M.E., Ghazvini M., Goffeau A., Gonzalez A., Grothues D., Guerreiro P., Hegemann J.H., Hewitt N., Hilger F., Hollenberg C.P., Horaitis O., Indge K.J., Jacquier A., James C.M., Jauniaux J.-C., Jimenez A., Keuchel H., Kirchrath L., Kleine K., Koetter P., Legrain P., Liebl S., Louis E.J., Maia e Silva A., Marck C., Monnier A.-L., Moestl D., Mueller S., Obermaier B., Oliver S.G., Pallier C., Pascolo S., Pfeiffer F., Philippsen P., Planta R.J., Pohl F.M., Pohl T.M., Poehlmann R., Portetelle D., Purnelle B., Puzos V., Ramezani Rad M., Rasmussen S.W., Remacha M.A., Revuelta J.L., Richard G.-F., Rieger M., Rodrigues-Pousada C., Rose M., Rupp T., Santos M.A., Schwager C., Sensen C., Skala J., Soares H., Sor F., Stegemann J., Tettelin H., Thierry A., Tzermia M., Urrestarazu L.A., van Dyck L., van Vliet-Reedijk J.C., Valens M., Vandenbol M., Vilela C., Vissers S., von Wettstein D., Voss H., Wiemann S., Xu G., Zimmermann J., Haasemann M., Becker I., Mewes H.-W.
    Nature 369:371-378(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  3. Cited for: GENOME REANNOTATION.
    Strain: ATCC 204508 / S288c.
  4. "A novel Hsp70 of the yeast ER lumen is required for the efficient translocation of a number of protein precursors."
    Craven R.A., Egerton M., Stirling C.J.
    EMBO J. 15:2640-2650(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: CHARACTERIZATION.
  5. "Functional and genomic analyses reveal an essential coordination between the unfolded protein response and ER-associated degradation."
    Travers K.J., Patil C.K., Wodicka L., Lockhart D.J., Weissman J.S., Walter P.
    Cell 101:249-258(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: INDUCTION.
  6. Cited for: LEVEL OF PROTEIN EXPRESSION.
  7. Cited for: FUNCTION, INTERACTION WITH KAR2, MUTAGENESIS OF GLY-239.

Entry informationi

Entry nameiLHS1_YEAST
AccessioniPrimary (citable) accession number: P36016
Secondary accession number(s): D6VXL3
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 1, 1994
Last sequence update: June 1, 1994
Last modified: February 4, 2015
This is version 136 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 137 molecules/cell in log phase SD medium.1 Publication

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  3. Yeast chromosome XI
    Yeast (Saccharomyces cerevisiae) chromosome XI: entries and gene names

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.