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P36016

- LHS1_YEAST

UniProt

P36016 - LHS1_YEAST

Protein

Heat shock protein 70 homolog LHS1

Gene

LHS1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 132 (01 Oct 2014)
      Sequence version 1 (01 Jun 1994)
      Previous versions | rss
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    Functioni

    Chaperone required for protein translocation and folding in the endoplasmic reticulum.1 Publication

    Catalytic activityi

    ATP + H2O = ADP + phosphate.

    GO - Molecular functioni

    1. adenyl-nucleotide exchange factor activity Source: SGD
    2. ATPase activity Source: UniProtKB-EC
    3. ATP binding Source: SGD
    4. unfolded protein binding Source: SGD

    GO - Biological processi

    1. posttranslational protein targeting to membrane, translocation Source: SGD
    2. regulation of catalytic activity Source: GOC
    3. response to unfolded protein Source: SGD

    Keywords - Molecular functioni

    Chaperone, Hydrolase

    Keywords - Ligandi

    ATP-binding, Nucleotide-binding

    Enzyme and pathway databases

    BioCyciYEAST:G3O-31869-MONOMER.
    ReactomeiREACT_188969. XBP1(S) activates chaperone genes.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Heat shock protein 70 homolog LHS1 (EC:3.6.1.3)
    Gene namesi
    Name:LHS1
    Ordered Locus Names:YKL073W
    ORF Names:YKL355
    OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
    Taxonomic identifieri559292 [NCBI]
    Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
    ProteomesiUP000002311: Chromosome XI

    Organism-specific databases

    CYGDiYKL073w.
    SGDiS000001556. LHS1.

    Subcellular locationi

    GO - Cellular componenti

    1. endoplasmic reticulum lumen Source: SGD

    Keywords - Cellular componenti

    Endoplasmic reticulum

    Pathology & Biotechi

    Mutagenesis

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Mutagenesisi239 – 2391G → D in allele LHS1-1; constitutive ATPase activity. 1 Publication

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Signal peptidei1 – 2020Sequence AnalysisAdd
    BLAST
    Chaini21 – 881861Heat shock protein 70 homolog LHS1PRO_0000013556Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Glycosylationi128 – 1281N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi458 – 4581N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi474 – 4741N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi481 – 4811N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi489 – 4891N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi527 – 5271N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi844 – 8441N-linked (GlcNAc...)Sequence Analysis

    Post-translational modificationi

    N-glycosylated.

    Keywords - PTMi

    Glycoprotein

    Proteomic databases

    MaxQBiP36016.
    PaxDbiP36016.
    PeptideAtlasiP36016.
    PRIDEiP36016.

    Expressioni

    Inductioni

    By the unfolded protein response (UPR).1 Publication

    Gene expression databases

    GenevestigatoriP36016.

    Interactioni

    Subunit structurei

    Interacts with the heat shock protein 70 (HSP70) KAR2, and this stimulates nucleotide exchange on KAR2. KAR2 in turn acts to stimulate the ATPase activity of LHS1.1 Publication

    Protein-protein interaction databases

    BioGridi34058. 139 interactions.
    DIPiDIP-6520N.
    IntActiP36016. 3 interactions.
    MINTiMINT-684320.
    STRINGi4932.YKL073W.

    Structurei

    3D structure databases

    ProteinModelPortaliP36016.
    SMRiP36016. Positions 20-671.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Motif

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Motifi878 – 8814Prevents secretion from ERPROSITE-ProRule annotation

    Sequence similaritiesi

    Belongs to the heat shock protein 70 family.Curated

    Keywords - Domaini

    Signal

    Phylogenomic databases

    eggNOGiCOG0443.
    GeneTreeiENSGT00750000118452.
    HOGENOMiHOG000111144.
    KOiK09486.
    OMAiIDYGQQF.
    OrthoDBiEOG7DC2CX.

    Family and domain databases

    Gene3Di1.20.1270.10. 1 hit.
    InterProiIPR018181. Heat_shock_70_CS.
    IPR029048. HSP70_C.
    IPR013126. Hsp_70_fam.
    [Graphical view]
    PfamiPF00012. HSP70. 1 hit.
    [Graphical view]
    PRINTSiPR00301. HEATSHOCK70.
    PROSITEiPS00014. ER_TARGET. 1 hit.
    PS00329. HSP70_2. 1 hit.
    PS01036. HSP70_3. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    P36016-1 [UniParc]FASTAAdd to Basket

    « Hide

    MRNVLRLLFL TAFVAIGSLA AVLGVDYGQQ NIKAIVVSPQ APLELVLTPE    50
    AKRKEISGLS IKRLPGYGKD DPNGIERIYG SAVGSLATRF PQNTLLHLKP 100
    LLGKSLEDET TVTLYSKQHP GLEMVSTNRS TIAFLVDNVE YPLEELVAMN 150
    VQEIANRANS LLKDRDARTE DFVNKMSFTI PDFFDQHQRK ALLDASSITT 200
    GIEETYLVSE GMSVAVNFVL KQRQFPPGEQ QHYIVYDMGS GSIKASMFSI 250
    LQPEDTTQPV TIEFEGYGYN PHLGGAKFTM DIGSLIENKF LETHPAIRTD 300
    ELHANPKALA KINQAAEKAK LILSANSEAS INIESLINDI DFRTSITRQE 350
    FEEFIADSLL DIVKPINDAV TKQFGGYGTN LPEINGVILA GGSSRIPIVQ 400
    DQLIKLVSEE KVLRNVNADE SAVNGVVMRG IKLSNSFKTK PLNVVDRSVN 450
    TYSFKLSNES ELYDVFTRGS AYPNKTSILT NTTDSIPNNF TIDLFENGKL 500
    FETITVNSGA IKNSYSSDKC SSGVAYNITF DLSSDRLFSI QEVNCICQSE 550
    NDIGNSKQIK NKGSRLAFTS EDVEIKRLSP SERSRLHEHI KLLDKQDKER 600
    FQFQENLNVL ESNLYDARNL LMDDEVMQNG PKSQVEELSE MVKVYLDWLE 650
    DASFDTDPED IVSRIREIGI LKKKIELYMD SAKEPLNSQQ FKGMLEEGHK 700
    LLQAIETHKN TVEEFLSQFE TEFADTIDNV REEFKKIKQP AYVSKALSTW 750
    EETLTSFKNS ISEIEKFLAK NLFGEDLREH LFEIKLQFDM YRTKLEEKLR 800
    LIKSGDESRL NEIKKLHLRN FRLQKRKEEK LKRKLEQEKS RNNNETESTV 850
    INSADDKTTI VNDKTTESNP SSEEDILHDE L 881
    Length:881
    Mass (Da):99,572
    Last modified:June 1, 1994 - v1
    Checksum:iACED092CA3A34785
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X75780 Genomic DNA. Translation: CAA53401.1.
    Z28073 Genomic DNA. Translation: CAA81910.1.
    BK006944 Genomic DNA. Translation: DAA09083.1.
    PIRiS37895.
    RefSeqiNP_012850.1. NM_001179639.1.

    Genome annotation databases

    EnsemblFungiiYKL073W; YKL073W; YKL073W.
    GeneIDi853789.
    KEGGisce:YKL073W.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X75780 Genomic DNA. Translation: CAA53401.1 .
    Z28073 Genomic DNA. Translation: CAA81910.1 .
    BK006944 Genomic DNA. Translation: DAA09083.1 .
    PIRi S37895.
    RefSeqi NP_012850.1. NM_001179639.1.

    3D structure databases

    ProteinModelPortali P36016.
    SMRi P36016. Positions 20-671.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 34058. 139 interactions.
    DIPi DIP-6520N.
    IntActi P36016. 3 interactions.
    MINTi MINT-684320.
    STRINGi 4932.YKL073W.

    Proteomic databases

    MaxQBi P36016.
    PaxDbi P36016.
    PeptideAtlasi P36016.
    PRIDEi P36016.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblFungii YKL073W ; YKL073W ; YKL073W .
    GeneIDi 853789.
    KEGGi sce:YKL073W.

    Organism-specific databases

    CYGDi YKL073w.
    SGDi S000001556. LHS1.

    Phylogenomic databases

    eggNOGi COG0443.
    GeneTreei ENSGT00750000118452.
    HOGENOMi HOG000111144.
    KOi K09486.
    OMAi IDYGQQF.
    OrthoDBi EOG7DC2CX.

    Enzyme and pathway databases

    BioCyci YEAST:G3O-31869-MONOMER.
    Reactomei REACT_188969. XBP1(S) activates chaperone genes.

    Miscellaneous databases

    NextBioi 974918.

    Gene expression databases

    Genevestigatori P36016.

    Family and domain databases

    Gene3Di 1.20.1270.10. 1 hit.
    InterProi IPR018181. Heat_shock_70_CS.
    IPR029048. HSP70_C.
    IPR013126. Hsp_70_fam.
    [Graphical view ]
    Pfami PF00012. HSP70. 1 hit.
    [Graphical view ]
    PRINTSi PR00301. HEATSHOCK70.
    PROSITEi PS00014. ER_TARGET. 1 hit.
    PS00329. HSP70_2. 1 hit.
    PS01036. HSP70_3. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Sequence of a 20.7 kb region of yeast chromosome XI includes the NUP100 gene, an open reading frame (ORF) possibly representing a nucleoside diphosphate kinase gene, tRNAs for His, Val and Trp in addition to seven ORFs with weak or no significant similarity to known proteins."
      Rasmussen S.W.
      Yeast 10:S69-S74(1994) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: ATCC 204508 / S288c.
    2. "Complete DNA sequence of yeast chromosome XI."
      Dujon B., Alexandraki D., Andre B., Ansorge W., Baladron V., Ballesta J.P.G., Banrevi A., Bolle P.-A., Bolotin-Fukuhara M., Bossier P., Bou G., Boyer J., Buitrago M.J., Cheret G., Colleaux L., Daignan-Fornier B., del Rey F., Dion C.
      , Domdey H., Duesterhoeft A., Duesterhus S., Entian K.-D., Erfle H., Esteban P.F., Feldmann H., Fernandes L., Fobo G.M., Fritz C., Fukuhara H., Gabel C., Gaillon L., Garcia-Cantalejo J.M., Garcia-Ramirez J.J., Gent M.E., Ghazvini M., Goffeau A., Gonzalez A., Grothues D., Guerreiro P., Hegemann J.H., Hewitt N., Hilger F., Hollenberg C.P., Horaitis O., Indge K.J., Jacquier A., James C.M., Jauniaux J.-C., Jimenez A., Keuchel H., Kirchrath L., Kleine K., Koetter P., Legrain P., Liebl S., Louis E.J., Maia e Silva A., Marck C., Monnier A.-L., Moestl D., Mueller S., Obermaier B., Oliver S.G., Pallier C., Pascolo S., Pfeiffer F., Philippsen P., Planta R.J., Pohl F.M., Pohl T.M., Poehlmann R., Portetelle D., Purnelle B., Puzos V., Ramezani Rad M., Rasmussen S.W., Remacha M.A., Revuelta J.L., Richard G.-F., Rieger M., Rodrigues-Pousada C., Rose M., Rupp T., Santos M.A., Schwager C., Sensen C., Skala J., Soares H., Sor F., Stegemann J., Tettelin H., Thierry A., Tzermia M., Urrestarazu L.A., van Dyck L., van Vliet-Reedijk J.C., Valens M., Vandenbol M., Vilela C., Vissers S., von Wettstein D., Voss H., Wiemann S., Xu G., Zimmermann J., Haasemann M., Becker I., Mewes H.-W.
      Nature 369:371-378(1994) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 204508 / S288c.
    3. Cited for: GENOME REANNOTATION.
      Strain: ATCC 204508 / S288c.
    4. "A novel Hsp70 of the yeast ER lumen is required for the efficient translocation of a number of protein precursors."
      Craven R.A., Egerton M., Stirling C.J.
      EMBO J. 15:2640-2650(1996) [PubMed] [Europe PMC] [Abstract]
      Cited for: CHARACTERIZATION.
    5. "Functional and genomic analyses reveal an essential coordination between the unfolded protein response and ER-associated degradation."
      Travers K.J., Patil C.K., Wodicka L., Lockhart D.J., Weissman J.S., Walter P.
      Cell 101:249-258(2000) [PubMed] [Europe PMC] [Abstract]
      Cited for: INDUCTION.
    6. Cited for: LEVEL OF PROTEIN EXPRESSION.
    7. Cited for: FUNCTION, INTERACTION WITH KAR2, MUTAGENESIS OF GLY-239.

    Entry informationi

    Entry nameiLHS1_YEAST
    AccessioniPrimary (citable) accession number: P36016
    Secondary accession number(s): D6VXL3
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: June 1, 1994
    Last sequence update: June 1, 1994
    Last modified: October 1, 2014
    This is version 132 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programFungal Protein Annotation Program

    Miscellaneousi

    Miscellaneous

    Present with 137 molecules/cell in log phase SD medium.1 Publication

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families
    2. Yeast
      Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
    3. Yeast chromosome XI
      Yeast (Saccharomyces cerevisiae) chromosome XI: entries and gene names

    External Data

    Dasty 3