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P36016 (LHS1_YEAST) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 128. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Heat shock protein 70 homolog LHS1

EC=3.6.1.3
Gene names
Name:LHS1
Ordered Locus Names:YKL073W
ORF Names:YKL355
OrganismSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]
Taxonomic identifier559292 [NCBI]
Taxonomic lineageEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces

Protein attributes

Sequence length881 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Chaperone required for protein translocation and folding in the endoplasmic reticulum. Ref.7

Catalytic activity

ATP + H2O = ADP + phosphate.

Subunit structure

Interacts with the heat shock protein 70 (HSP70) KAR2, and this stimulates nucleotide exchange on KAR2. KAR2 in turn acts to stimulate the ATPase activity of LHS1. Ref.7

Subcellular location

Endoplasmic reticulum lumen.

Induction

By the unfolded protein response (UPR). Ref.5

Post-translational modification

N-glycosylated.

Miscellaneous

Present with 137 molecules/cell in log phase SD medium. Ref.6

Sequence similarities

Belongs to the heat shock protein 70 family.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 2020 Potential
Chain21 – 881861Heat shock protein 70 homolog LHS1
PRO_0000013556

Regions

Motif878 – 8814Prevents secretion from ER Potential

Amino acid modifications

Glycosylation1281N-linked (GlcNAc...) Potential
Glycosylation4581N-linked (GlcNAc...) Potential
Glycosylation4741N-linked (GlcNAc...) Potential
Glycosylation4811N-linked (GlcNAc...) Potential
Glycosylation4891N-linked (GlcNAc...) Potential
Glycosylation5271N-linked (GlcNAc...) Potential
Glycosylation8441N-linked (GlcNAc...) Potential

Experimental info

Mutagenesis2391G → D in allele LHS1-1; constitutive ATPase activity. Ref.7

Sequences

Sequence LengthMass (Da)Tools
P36016 [UniParc].

Last modified June 1, 1994. Version 1.
Checksum: ACED092CA3A34785

FASTA88199,572
        10         20         30         40         50         60 
MRNVLRLLFL TAFVAIGSLA AVLGVDYGQQ NIKAIVVSPQ APLELVLTPE AKRKEISGLS 

        70         80         90        100        110        120 
IKRLPGYGKD DPNGIERIYG SAVGSLATRF PQNTLLHLKP LLGKSLEDET TVTLYSKQHP 

       130        140        150        160        170        180 
GLEMVSTNRS TIAFLVDNVE YPLEELVAMN VQEIANRANS LLKDRDARTE DFVNKMSFTI 

       190        200        210        220        230        240 
PDFFDQHQRK ALLDASSITT GIEETYLVSE GMSVAVNFVL KQRQFPPGEQ QHYIVYDMGS 

       250        260        270        280        290        300 
GSIKASMFSI LQPEDTTQPV TIEFEGYGYN PHLGGAKFTM DIGSLIENKF LETHPAIRTD 

       310        320        330        340        350        360 
ELHANPKALA KINQAAEKAK LILSANSEAS INIESLINDI DFRTSITRQE FEEFIADSLL 

       370        380        390        400        410        420 
DIVKPINDAV TKQFGGYGTN LPEINGVILA GGSSRIPIVQ DQLIKLVSEE KVLRNVNADE 

       430        440        450        460        470        480 
SAVNGVVMRG IKLSNSFKTK PLNVVDRSVN TYSFKLSNES ELYDVFTRGS AYPNKTSILT 

       490        500        510        520        530        540 
NTTDSIPNNF TIDLFENGKL FETITVNSGA IKNSYSSDKC SSGVAYNITF DLSSDRLFSI 

       550        560        570        580        590        600 
QEVNCICQSE NDIGNSKQIK NKGSRLAFTS EDVEIKRLSP SERSRLHEHI KLLDKQDKER 

       610        620        630        640        650        660 
FQFQENLNVL ESNLYDARNL LMDDEVMQNG PKSQVEELSE MVKVYLDWLE DASFDTDPED 

       670        680        690        700        710        720 
IVSRIREIGI LKKKIELYMD SAKEPLNSQQ FKGMLEEGHK LLQAIETHKN TVEEFLSQFE 

       730        740        750        760        770        780 
TEFADTIDNV REEFKKIKQP AYVSKALSTW EETLTSFKNS ISEIEKFLAK NLFGEDLREH 

       790        800        810        820        830        840 
LFEIKLQFDM YRTKLEEKLR LIKSGDESRL NEIKKLHLRN FRLQKRKEEK LKRKLEQEKS 

       850        860        870        880 
RNNNETESTV INSADDKTTI VNDKTTESNP SSEEDILHDE L 

« Hide

References

« Hide 'large scale' references
[1]"Sequence of a 20.7 kb region of yeast chromosome XI includes the NUP100 gene, an open reading frame (ORF) possibly representing a nucleoside diphosphate kinase gene, tRNAs for His, Val and Trp in addition to seven ORFs with weak or no significant similarity to known proteins."
Rasmussen S.W.
Yeast 10:S69-S74(1994) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: ATCC 204508 / S288c.
[2]"Complete DNA sequence of yeast chromosome XI."
Dujon B., Alexandraki D., Andre B., Ansorge W., Baladron V., Ballesta J.P.G., Banrevi A., Bolle P.-A., Bolotin-Fukuhara M., Bossier P., Bou G., Boyer J., Buitrago M.J., Cheret G., Colleaux L., Daignan-Fornier B., del Rey F., Dion C. expand/collapse author list , Domdey H., Duesterhoeft A., Duesterhus S., Entian K.-D., Erfle H., Esteban P.F., Feldmann H., Fernandes L., Fobo G.M., Fritz C., Fukuhara H., Gabel C., Gaillon L., Garcia-Cantalejo J.M., Garcia-Ramirez J.J., Gent M.E., Ghazvini M., Goffeau A., Gonzalez A., Grothues D., Guerreiro P., Hegemann J.H., Hewitt N., Hilger F., Hollenberg C.P., Horaitis O., Indge K.J., Jacquier A., James C.M., Jauniaux J.-C., Jimenez A., Keuchel H., Kirchrath L., Kleine K., Koetter P., Legrain P., Liebl S., Louis E.J., Maia e Silva A., Marck C., Monnier A.-L., Moestl D., Mueller S., Obermaier B., Oliver S.G., Pallier C., Pascolo S., Pfeiffer F., Philippsen P., Planta R.J., Pohl F.M., Pohl T.M., Poehlmann R., Portetelle D., Purnelle B., Puzos V., Ramezani Rad M., Rasmussen S.W., Remacha M.A., Revuelta J.L., Richard G.-F., Rieger M., Rodrigues-Pousada C., Rose M., Rupp T., Santos M.A., Schwager C., Sensen C., Skala J., Soares H., Sor F., Stegemann J., Tettelin H., Thierry A., Tzermia M., Urrestarazu L.A., van Dyck L., van Vliet-Reedijk J.C., Valens M., Vandenbol M., Vilela C., Vissers S., von Wettstein D., Voss H., Wiemann S., Xu G., Zimmermann J., Haasemann M., Becker I., Mewes H.-W.
Nature 369:371-378(1994) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 204508 / S288c.
[3]Saccharomyces Genome Database
Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases
Cited for: GENOME REANNOTATION.
Strain: ATCC 204508 / S288c.
[4]"A novel Hsp70 of the yeast ER lumen is required for the efficient translocation of a number of protein precursors."
Craven R.A., Egerton M., Stirling C.J.
EMBO J. 15:2640-2650(1996) [PubMed] [Europe PMC] [Abstract]
Cited for: CHARACTERIZATION.
[5]"Functional and genomic analyses reveal an essential coordination between the unfolded protein response and ER-associated degradation."
Travers K.J., Patil C.K., Wodicka L., Lockhart D.J., Weissman J.S., Walter P.
Cell 101:249-258(2000) [PubMed] [Europe PMC] [Abstract]
Cited for: INDUCTION.
[6]"Global analysis of protein expression in yeast."
Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S.
Nature 425:737-741(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: LEVEL OF PROTEIN EXPRESSION.
[7]"Coordinated activation of Hsp70 chaperones."
Steel G.J., Fullerton D.M., Tyson J.R., Stirling C.J.
Science 303:98-101(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, INTERACTION WITH KAR2, MUTAGENESIS OF GLY-239.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
X75780 Genomic DNA. Translation: CAA53401.1.
Z28073 Genomic DNA. Translation: CAA81910.1.
BK006944 Genomic DNA. Translation: DAA09083.1.
PIRS37895.
RefSeqNP_012850.1. NM_001179639.1.

3D structure databases

ProteinModelPortalP36016.
SMRP36016. Positions 19-671.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid34058. 139 interactions.
DIPDIP-6520N.
IntActP36016. 3 interactions.
MINTMINT-684320.
STRING4932.YKL073W.

Proteomic databases

PaxDbP36016.
PeptideAtlasP36016.
PRIDEP36016.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblFungiYKL073W; YKL073W; YKL073W.
GeneID853789.
KEGGsce:YKL073W.

Organism-specific databases

CYGDYKL073w.
SGDS000001556. LHS1.

Phylogenomic databases

eggNOGCOG0443.
GeneTreeENSGT00750000118452.
HOGENOMHOG000111144.
KOK09486.
OMAIDYGQQF.
OrthoDBEOG7DC2CX.

Enzyme and pathway databases

BioCycYEAST:G3O-31869-MONOMER.

Gene expression databases

GenevestigatorP36016.

Family and domain databases

InterProIPR018181. Heat_shock_70_CS.
IPR013126. Hsp_70_fam.
[Graphical view]
PfamPF00012. HSP70. 1 hit.
[Graphical view]
PRINTSPR00301. HEATSHOCK70.
PROSITEPS00014. ER_TARGET. 1 hit.
PS00329. HSP70_2. 1 hit.
PS01036. HSP70_3. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio974918.

Entry information

Entry nameLHS1_YEAST
AccessionPrimary (citable) accession number: P36016
Secondary accession number(s): D6VXL3
Entry history
Integrated into UniProtKB/Swiss-Prot: June 1, 1994
Last sequence update: June 1, 1994
Last modified: April 16, 2014
This is version 128 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Relevant documents

Yeast chromosome XI

Yeast (Saccharomyces cerevisiae) chromosome XI: entries and gene names

Yeast

Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD

SIMILARITY comments

Index of protein domains and families