P36015 (YKT6_YEAST) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Synaptobrevin homolog YKT6
EC=2.3.1.-

Gene names

Name:	YKT6
Ordered Locus Names:	YKL196C

Organism

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)

Taxonomic identifier

559292 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces

Protein attributes

Sequence length	200 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Subcellular location	Cell membrane; Lipid-anchor; Cytoplasmic side Potential.
Sequence similarities	Belongs to the synaptobrevin family. Contains 1 longin domain. Contains 1 v-SNARE coiled-coil homology domain.

Ontologies

Keywords
Cellular component	Cell membrane Membrane
Domain	Coiled coil
Molecular function	Transferase
PTM	Lipoprotein Methylation Palmitate Phosphoprotein Prenylation
Technical term	3D-structure Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	intra-Golgi vesicle-mediated transport Traceable author statement. Source: SGD vacuole fusion, non-autophagic Inferred from mutant phenotype. Source: SGD vesicle fusion Inferred from direct assay. Source: SGD
Cellular component	Golgi apparatus Inferred from direct assay. Source: SGD SNARE complex Inferred from direct assay. Source: SGD endosome Inferred from direct assay. Source: SGD fungal-type vacuole Inferred from direct assay. Source: SGD integral to membrane Inferred from electronic annotation. Source: InterPro mitochondrion Inferred from direct assay. Source: SGD plasma membrane Inferred from electronic annotation. Source: UniProtKB-SubCell soluble fraction Inferred from direct assay. Source: SGD
Molecular function	SNAP receptor activity Inferred from direct assay. Source: SGD palmitoyltransferase activity Inferred from direct assay. Source: SGD
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
PEP12	P32854	2	EBI-26982,EBI-13098
VTI1	Q04338	6	EBI-26982,EBI-20519

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 197

197

Synaptobrevin homolog YKT6

PRO_0000206781

Propeptide

198 – 200

3

Removed in mature form By similarity

PRO_0000396675

Regions

Domain

7 – 129

123

Longin

Domain

140 – 200

61

v-SNARE coiled-coil homology

Amino acid modifications

Modified residue

158

1

Phosphothreonine Ref.4 Ref.6

Modified residue

176

1

Phosphoserine Ref.6

Modified residue

178

1

Phosphoserine Ref.5 Ref.6

Modified residue

180

1

Phosphothreonine Ref.6

Modified residue

182

1

Phosphoserine Ref.5 Ref.6

Modified residue

197

1

Cysteine methyl ester By similarity

Lipidation

196

1

S-palmitoyl cysteine By similarity

Lipidation

197

1

S-farnesyl cysteine By similarity

Secondary structure

1

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200

Helix Strand Turn

Details...

Beta strand

3 – 10

8

Beta strand

13 – 15

3

Beta strand

17 – 23

7

Turn

30 – 34

5

Helix

35 – 50

16

Beta strand

55 – 61

7

Beta strand

64 – 70

7

Beta strand

74 – 82

9

Helix

87 – 104

18

Helix

107 – 109

3

Turn

110 – 112

3

Helix

118 – 120

3

Helix

124 – 131

8

Sequences

	Sequence		Length	Mass (Da)
	P36015 [UniParc]. Last modified June 1, 1994. Version 1. Checksum: 9BA0E47ED87B096D	FASTA	200	22,707
	10 20 30 40 50 60 MRIYYIGVFR SGGEKALELS EVKDLSQFGF FERSSVGQFM TFFAETVASR TGAGQRQSIE 70 80 90 100 110 120 EGNYIGHVYA RSEGICGVLI TDKEYPVRPA YTLLNKILDE YLVAHPKEEW ADVTETNDAL 130 140 150 160 170 180 KMKQLDTYIS KYQDPSQADA IMKVQQELDE TKIVLHKTIE NVLQRGEKLD NLVDKSESLT 190 200 ASSKMFYKQA KKSNSCCIIM

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Complete DNA sequence of yeast chromosome XI." Dujon B., Alexandraki D., Andre B., Ansorge W., Baladron V., Ballesta J.P.G., Banrevi A., Bolle P.-A., Bolotin-Fukuhara M., Bossier P., Bou G., Boyer J., Buitrago M.J., Cheret G., Colleaux L., Daignan-Fornier B., del Rey F., Dion C. , Domdey H., Duesterhoeft A., Duesterhus S., Entian K.-D., Erfle H., Esteban P.F., Feldmann H., Fernandes L., Fobo G.M., Fritz C., Fukuhara H., Gabel C., Gaillon L., Garcia-Cantalejo J.M., Garcia-Ramirez J.J., Gent M.E., Ghazvini M., Goffeau A., Gonzalez A., Grothues D., Guerreiro P., Hegemann J.H., Hewitt N., Hilger F., Hollenberg C.P., Horaitis O., Indge K.J., Jacquier A., James C.M., Jauniaux J.-C., Jimenez A., Keuchel H., Kirchrath L., Kleine K., Koetter P., Legrain P., Liebl S., Louis E.J., Maia e Silva A., Marck C., Monnier A.-L., Moestl D., Mueller S., Obermaier B., Oliver S.G., Pallier C., Pascolo S., Pfeiffer F., Philippsen P., Planta R.J., Pohl F.M., Pohl T.M., Poehlmann R., Portetelle D., Purnelle B., Puzos V., Ramezani Rad M., Rasmussen S.W., Remacha M.A., Revuelta J.L., Richard G.-F., Rieger M., Rodrigues-Pousada C., Rose M., Rupp T., Santos M.A., Schwager C., Sensen C., Skala J., Soares H., Sor F., Stegemann J., Tettelin H., Thierry A., Tzermia M., Urrestarazu L.A., van Dyck L., van Vliet-Reedijk J.C., Valens M., Vandenbol M., Vilela C., Vissers S., von Wettstein D., Voss H., Wiemann S., Xu G., Zimmermann J., Haasemann M., Becker I., Mewes H.-W. Nature 369:371-378(1994) [PubMed: 8196765] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 96604 / S288c / FY1679.
[2]	Saccharomyces Genome Database Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: ATCC 204508 / S288c.
[3]	"Approaching a complete repository of sequence-verified protein-encoding clones for Saccharomyces cerevisiae." Hu Y., Rolfs A., Bhullar B., Murthy T.V.S., Zhu C., Berger M.F., Camargo A.A., Kelley F., McCarron S., Jepson D., Richardson A., Raphael J., Moreira D., Taycher E., Zuo D., Mohr S., Kane M.F., Williamson J. , Simpson A.J.G., Bulyk M.L., Harlow E., Marsischky G., Kolodner R.D., LaBaer J. Genome Res. 17:536-543(2007) [PubMed: 17322287] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 204508 / S288c.
[4]	"Large-scale phosphorylation analysis of alpha-factor-arrested Saccharomyces cerevisiae." Li X., Gerber S.A., Rudner A.D., Beausoleil S.A., Haas W., Villen J., Elias J.E., Gygi S.P. J. Proteome Res. 6:1190-1197(2007) [PubMed: 17330950] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-158, MASS SPECTROMETRY. Strain: ADR376.
[5]	"Proteome-wide identification of in vivo targets of DNA damage checkpoint kinases." Smolka M.B., Albuquerque C.P., Chen S.H., Zhou H. Proc. Natl. Acad. Sci. U.S.A. 104:10364-10369(2007) [PubMed: 17563356] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-178 AND SER-182, MASS SPECTROMETRY.
[6]	"A multidimensional chromatography technology for in-depth phosphoproteome analysis." Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H. Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed: 18407956] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-158; SER-176; SER-178; THR-180 AND SER-182, MASS SPECTROMETRY.
[7]	"An autoinhibitory mechanism for nonsyntaxin SNARE proteins revealed by the structure of Ykt6p." Tochio H., Tsui M.M., Banfield D.K., Zhang M. Science 293:698-702(2001) [PubMed: 11474112] [Abstract] Cited for: STRUCTURE BY NMR OF 1-140.
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

Z28196 Genomic DNA. Translation: CAA82040.1.
AY558393 Genomic DNA. Translation: AAS56719.1.
BK006944 Genomic DNA. Translation: DAA08970.1.

PIR

S38033.

RefSeq

NP_012725.1. NM_001179762.1.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
1H8M	NMR	-	A	1-140	[»]
1IOU	NMR	-	A	1-140	[»]
3BW6	X-ray	2.50	A	1-140	[»]

ProteinModelPortal

P36015.

SMR

P36015. Positions 1-194.

ModBase

Search...

Protein-protein interaction databases

DIP

DIP-2247N.

IntAct

P36015. 25 interactions.

MINT

MINT-646403.

STRING

P36015.

Proteomic databases

PeptideAtlas

P36015.

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Genome annotation databases

EnsemblFungi

YKL196C; YKL196C; YKL196C.

GeneID

853638.

KEGG

sce:YKL196C.

NMPDR

fig|4932.3.peg.3702.

Organism-specific databases

CYGD

YKL196c.

SGD

S000001679. YKT6.

Phylogenomic databases

eggNOG

fuNOG07914.

GeneTree

EFGT00050000004851.

HOGENOM

HBG324813.

OMA

AFYKTAK.

OrthoDB

EOG46QB2Q.

Gene expression databases

ArrayExpress

P36015.

Genevestigator

P36015.

GermOnline

YKL196C. Saccharomyces cerevisiae.

Family and domain databases

InterPro

IPR010908. Longin.
IPR011012. Longin-like.
IPR001388. Synaptobrevin.
[Graphical view]

Gene3D

G3DSA:3.30.450.50. Longin. 1 hit.

KO

K08516.

Pfam

PF00957. Synaptobrevin. 1 hit.
[Graphical view]

PRINTS

PR00219. SYNAPTOBREVN.

SUPFAM

SSF64356. Longin_like. 1 hit.

PROSITE

PS50859. LONGIN. 1 hit.
PS00417. SYNAPTOBREVIN. 1 hit.
PS50892. V_SNARE. 1 hit.
[Graphical view]

ProtoNet

Search...

Other

NextBio

974525.

Entry information

Entry name

YKT6_YEAST

Accession

Primary (citable) accession number: P36015
Secondary accession number(s): D6VX04

Entry history

Integrated into UniProtKB/Swiss-Prot:	June 1, 1994
Last sequence update:	June 1, 1994
Last modified:	December 14, 2011
This is version 120 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program