Reviewed, UniProtKB/Swiss-Prot P36015 (YKT6_YEAST)

Names and origin

Protein names

Recommended name:
    Synaptobrevin homolog YKT6
    EC=2.3.1.-

Gene names

Name:	YKT6
Ordered Locus Names:	YKL196C

Organism

Saccharomyces cerevisiae (Baker's yeast) [Complete proteome]

Taxonomic identifier

4932 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces

Protein attributes

Sequence length	200 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Subcellular location	Cell membrane; Lipid-anchor; Cytoplasmic side Potential.
Sequence similarities	Belongs to the synaptobrevin family. Contains 1 longin domain. Contains 1 v-SNARE coiled-coil homology domain.

Ontologies

Keywords
Cellular component	Cell membrane Membrane
Domain	Coiled coil
Molecular function	Transferase
PTM	Lipoprotein Phosphoprotein Prenylation
Technical term	3D-structure Complete proteome
Gene Ontology (GO)
Biological process	intra-Golgi vesicle-mediated transport Traceable author statement. Source: SGD vesicle fusion Traceable author statement. Source: SGD
Cellular component	fungal-type vacuole Inferred from direct assay. Source: SGD integral to membrane Inferred from electronic annotation. Source: InterPro mitochondrion Inferred from direct assay. Source: SGD plasma membrane Inferred from electronic annotation. Source: UniProtKB-KW soluble fraction Inferred from direct assay. Source: SGD
Molecular function	SNAP receptor activity Traceable author statement. Source: SGD palmitoyltransferase activity Inferred from direct assay. Source: SGD
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
PEP12	P32854	1	EBI-26982,EBI-13098

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 200

200

Synaptobrevin homolog YKT6

PRO_0000206781

Regions

Domain

7 – 129

123

Longin

Domain

140 – 200

61

v-SNARE coiled-coil homology

Amino acid modifications

Modified residue

158

1

Phosphothreonine Ref.3 Ref.5

Modified residue

176

1

Phosphoserine Ref.5

Modified residue

178

1

Phosphoserine Ref.5 Ref.4

Modified residue

180

1

Phosphothreonine Ref.5

Modified residue

182

1

Phosphoserine Ref.5 Ref.4

Lipidation

197

1

S-farnesyl cysteine By similarity

Secondary structure

1

.

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200

Helix Strand Turn

Details...

Beta strand

3 – 10

8

Beta strand

13 – 15

3

Beta strand

17 – 23

7

Turn

30 – 34

5

Helix

35 – 50

16

Beta strand

55 – 61

7

Beta strand

64 – 70

7

Beta strand

74 – 82

9

Helix

87 – 104

18

Helix

107 – 109

3

Turn

110 – 112

3

Helix

118 – 120

3

Helix

124 – 131

8

Sequences

	Sequence		Length	Mass (Da)
	P36015-1 [UniParc]. Last modified June 1, 1994. Version 1. Checksum: 9BA0E47ED87B096D	FASTA	200	22,707
	10 20 30 40 50 60 MRIYYIGVFR SGGEKALELS EVKDLSQFGF FERSSVGQFM TFFAETVASR TGAGQRQSIE 70 80 90 100 110 120 EGNYIGHVYA RSEGICGVLI TDKEYPVRPA YTLLNKILDE YLVAHPKEEW ADVTETNDAL 130 140 150 160 170 180 KMKQLDTYIS KYQDPSQADA IMKVQQELDE TKIVLHKTIE NVLQRGEKLD NLVDKSESLT 190 200 ASSKMFYKQA KKSNSCCIIM

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Complete DNA sequence of yeast chromosome XI." Dujon B., Alexandraki D., Andre B., Ansorge W., Baladron V., Ballesta J.P.G., Banrevi A., Bolle P.-A., Bolotin-Fukuhara M., Bossier P., Bou G., Boyer J., Buitrago M.J., Cheret G., Colleaux L., Daignan-Fornier B., del Rey F., Dion C. , Domdey H., Duesterhoeft A., Duesterhus S., Entian K.-D., Erfle H., Esteban P.F., Feldmann H., Fernandes L., Fobo G.M., Fritz C., Fukuhara H., Gabel C., Gaillon L., Garcia-Cantalejo J.M., Garcia-Ramirez J.J., Gent M.E., Ghazvini M., Goffeau A., Gonzalez A., Grothues D., Guerreiro P., Hegemann J.H., Hewitt N., Hilger F., Hollenberg C.P., Horaitis O., Indge K.J., Jacquier A., James C.M., Jauniaux J.-C., Jimenez A., Keuchel H., Kirchrath L., Kleine K., Koetter P., Legrain P., Liebl S., Louis E.J., Maia e Silva A., Marck C., Monnier A.-L., Moestl D., Mueller S., Obermaier B., Oliver S.G., Pallier C., Pascolo S., Pfeiffer F., Philippsen P., Planta R.J., Pohl F.M., Pohl T.M., Poehlmann R., Portetelle D., Purnelle B., Puzos V., Ramezani Rad M., Rasmussen S.W., Remacha M.A., Revuelta J.L., Richard G.-F., Rieger M., Rodrigues-Pousada C., Rose M., Rupp T., Santos M.A., Schwager C., Sensen C., Skala J., Soares H., Sor F., Stegemann J., Tettelin H., Thierry A., Tzermia M., Urrestarazu L.A., van Dyck L., van Vliet-Reedijk J.C., Valens M., Vandenbol M., Vilela C., Vissers S., von Wettstein D., Voss H., Wiemann S., Xu G., Zimmermann J., Haasemann M., Becker I., Mewes H.-W. Nature 369:371-378(1994) [PubMed: 8196765] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 96604 / S288c / FY1679.
[2]	"Approaching a complete repository of sequence-verified protein-encoding clones for Saccharomyces cerevisiae." Hu Y., Rolfs A., Bhullar B., Murthy T.V.S., Zhu C., Berger M.F., Camargo A.A., Kelley F., McCarron S., Jepson D., Richardson A., Raphael J., Moreira D., Taycher E., Zuo D., Mohr S., Kane M.F., Williamson J. , Simpson A.J.G., Bulyk M.L., Harlow E., Marsischky G., Kolodner R.D., LaBaer J. Genome Res. 17:536-543(2007) [PubMed: 17322287] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 204508 / S288c.
[3]	"Large-scale phosphorylation analysis of alpha-factor-arrested Saccharomyces cerevisiae." Li X., Gerber S.A., Rudner A.D., Beausoleil S.A., Haas W., Villen J., Elias J.E., Gygi S.P. J. Proteome Res. 6:1190-1197(2007) [PubMed: 17330950] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-158, MASS SPECTROMETRY.
[4]	"Proteome-wide identification of in vivo targets of DNA damage checkpoint kinases." Smolka M.B., Albuquerque C.P., Chen S.H., Zhou H. Proc. Natl. Acad. Sci. U.S.A. 104:10364-10369(2007) [PubMed: 17563356] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-178 AND SER-182, MASS SPECTROMETRY.
[5]	"A multidimensional chromatography technology for in-depth phosphoproteome analysis." Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H. Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed: 18407956] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-158; SER-176; SER-178; THR-180 AND SER-182, MASS SPECTROMETRY.
[6]	"An autoinhibitory mechanism for nonsyntaxin SNARE proteins revealed by the structure of Ykt6p." Tochio H., Tsui M.M., Banfield D.K., Zhang M. Science 293:698-702(2001) [PubMed: 11474112] [Abstract] Cited for: STRUCTURE BY NMR OF 1-140.
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBLGenBankDDBJ

Z28196 Genomic DNA. Translation: CAA82040.1.
AY558393 Genomic DNA. Translation: AAS56719.1.

PIR

S38033.

RefSeq

NP_012725.1.

3D structure databases

PDBPDBe

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
1H8M	NMR	-	A	1-140	[»]
1IOU	NMR	-	A	1-140	[»]
3BW6	X-ray	2.50	A	1-140	[»]

ModBase

Search...

Protein-protein interaction databases

DIP

DIP:2247N.

IntAct

P36015. 15 interactions.

STRING

P36015.

Proteomic databases

PeptideAtlas

P36015.

Genome annotation databases

Ensembl

YKL196C; YKL196C; YKL196C; Saccharomyces cerevisiae. [Genome view]

GeneID

853638.

KEGG

sce:YKL196C.

NMPDR

fig|4932.3.peg.3702.

Organism-specific databases

CYGD

YKL196c.

SGD

S000001679. YKT6.

Phylogenomic databases

HOGENOM

P36015.

OMA

KQNSCCE

OrthoDB

EOG9NGJ41

Gene expression databases

ArrayExpress

P36015.

Genevestigator

P36015.

GermOnline

YKL196C. Saccharomyces cerevisiae.

Family and domain databases

InterPro

IPR010908. Longin.
IPR011012. Longin-like.
IPR001388. Synaptobrevin.
[Graphical view]

Gene3D

G3DSA:3.30.450.50. Longin. 1 hit.

Pfam

PF00957. Synaptobrevin. 1 hit.
[Graphical view]

PRINTS

PR00219. SYNAPTOBREVN.

PROSITE

PS50859. LONGIN. 1 hit.
PS00417. SYNAPTOBREVIN. 1 hit.
PS50892. V_SNARE. 1 hit.
[Graphical view]

ProtoNet

Search...

Other Resources

NextBio

974525.

Entry information

Entry name

YKT6_YEAST

Accession

Primary (citable) accession number: P36015

Entry history

Integrated into UniProtKB/Swiss-Prot:	June 1, 1994
Last sequence update:	June 1, 1994
Last modified:	November 24, 2009
This is version 100 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

FPAP (Fungal Proteome Annotation Project)