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Protein

NAD-dependent malic enzyme, mitochondrial

Gene

MAE1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Catalytic activityi

(S)-malate + NAD+ = pyruvate + CO2 + NADH.
Oxaloacetate = pyruvate + CO2.

Cofactori

Mg2+By similarity, Mn2+By similarityNote: Divalent metal cations. Prefers magnesium or manganese.By similarity

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei187 – 1871Proton donorBy similarity
Active sitei259 – 2591Proton acceptorBy similarity
Metal bindingi330 – 3301Divalent metal cationBy similarity
Metal bindingi331 – 3311Divalent metal cationBy similarity
Metal bindingi354 – 3541Divalent metal cationBy similarity
Binding sitei354 – 3541NADBy similarity
Sitei354 – 3541Important for activityBy similarity
Binding sitei499 – 4991NADBy similarity

GO - Molecular functioni

GO - Biological processi

  • cellular amino acid metabolic process Source: SGD
  • malate metabolic process Source: InterPro
  • pyruvate metabolic process Source: SGD
Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Ligandi

Metal-binding, NAD

Enzyme and pathway databases

BioCyciMetaCyc:YKL029C-MONOMER.
YEAST:YKL029C-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
NAD-dependent malic enzyme, mitochondrial (EC:1.1.1.38)
Short name:
NAD-ME
Gene namesi
Name:MAE1
Ordered Locus Names:YKL029C
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome XI

Organism-specific databases

EuPathDBiFungiDB:YKL029C.
SGDiS000001512. MAE1.

Subcellular locationi

GO - Cellular componenti

  • mitochondrial matrix Source: UniProtKB-SubCell
  • mitochondrion Source: SGD
Complete GO annotation...

Keywords - Cellular componenti

Mitochondrion

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini? – 669NAD-dependent malic enzyme, mitochondrialPRO_0000160206
Transit peptidei1 – ?Mitochondrion

Proteomic databases

MaxQBiP36013.

Interactioni

Protein-protein interaction databases

BioGridi34102. 121 interactions.
DIPiDIP-4444N.
IntActiP36013. 71 interactions.
MINTiMINT-572667.

Structurei

3D structure databases

ProteinModelPortaliP36013.
SMRiP36013. Positions 99-661.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the malic enzymes family.Curated

Keywords - Domaini

Transit peptide

Phylogenomic databases

GeneTreeiENSGT00390000000754.
HOGENOMiHOG000042487.
InParanoidiP36013.
KOiK00027.
OMAiIRCLDAY.
OrthoDBiEOG092C16UJ.

Family and domain databases

Gene3Di3.40.50.10380. 1 hit.
3.40.50.720. 1 hit.
InterProiIPR015884. Malic_enzyme_CS.
IPR012301. Malic_N_dom.
IPR012302. Malic_NAD-bd.
IPR001891. Malic_OxRdtase.
IPR016040. NAD(P)-bd_dom.
[Graphical view]
PfamiPF00390. malic. 1 hit.
PF03949. Malic_M. 1 hit.
[Graphical view]
PIRSFiPIRSF000106. ME. 1 hit.
PRINTSiPR00072. MALOXRDTASE.
SMARTiSM01274. malic. 1 hit.
SM00919. Malic_M. 1 hit.
[Graphical view]
SUPFAMiSSF51735. SSF51735. 1 hit.
PROSITEiPS00331. MALIC_ENZYMES. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P36013-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MLRTRLSVSV AARSQLTRSL TASRTAPLRR WPIQQSRLYS SNTRSHKATT
60 70 80 90 100
TRENTFQKPY SDEEVTKTPV GSRARKIFEA PHPHATRLTV EGAIECPLES
110 120 130 140 150
FQLLNSPLFN KGSAFTQEER EAFNLEALLP PQVNTLDEQL ERSYKQLCYL
160 170 180 190 200
KTPLAKNDFM TSLRVQNKVL YFALIRRHIK ELVPIIYTPT EGDAIAAYSH
210 220 230 240 250
RFRKPEGVFL DITEPDSIEC RLATYGGDKD VDYIVVSDSE GILGIGDQGI
260 270 280 290 300
GGVRIAISKL ALMTLCGGIH PGRVLPVCLD VGTNNKKLAR DELYMGNKFS
310 320 330 340 350
RIRGKQYDDF LEKFIKAVKK VYPSAVLHFE DFGVKNARRL LEKYRYELPS
360 370 380 390 400
FNDDIQGTGA VVMASLIAAL KHTNRDLKDT RVLIYGAGSA GLGIADQIVN
410 420 430 440 450
HMVTHGVDKE EARKKIFLMD RRGLILQSYE ANSTPAQHVY AKSDAEWAGI
460 470 480 490 500
NTRSLHDVVE NVKPTCLVGC STQAGAFTQD VVEEMHKHNP RPIIFPLSNP
510 520 530 540 550
TRLHEAVPAD LMKWTNNNAL VATGSPFPPV DGYRISENNN CYSFPGIGLG
560 570 580 590 600
AVLSRATTIT DKMISAAVDQ LAELSPLREG DSRPGLLPGL DTITNTSARL
610 620 630 640 650
ATAVILQALE EGTARIEQEQ VPGGAPGETV KVPRDFDECL QWVKAQMWEP
660
VYRPMIKVQH DPSVHTNQL
Length:669
Mass (Da):74,376
Last modified:June 1, 1994 - v1
Checksum:i0122B52E846B748F
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z28029 Genomic DNA. Translation: CAA81865.1.
BK006944 Genomic DNA. Translation: DAA09125.1.
PIRiS37846.
RefSeqiNP_012896.1. NM_001179595.1.

Genome annotation databases

EnsemblFungiiYKL029C; YKL029C; YKL029C.
GeneIDi853839.
KEGGisce:YKL029C.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z28029 Genomic DNA. Translation: CAA81865.1.
BK006944 Genomic DNA. Translation: DAA09125.1.
PIRiS37846.
RefSeqiNP_012896.1. NM_001179595.1.

3D structure databases

ProteinModelPortaliP36013.
SMRiP36013. Positions 99-661.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi34102. 121 interactions.
DIPiDIP-4444N.
IntActiP36013. 71 interactions.
MINTiMINT-572667.

Proteomic databases

MaxQBiP36013.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYKL029C; YKL029C; YKL029C.
GeneIDi853839.
KEGGisce:YKL029C.

Organism-specific databases

EuPathDBiFungiDB:YKL029C.
SGDiS000001512. MAE1.

Phylogenomic databases

GeneTreeiENSGT00390000000754.
HOGENOMiHOG000042487.
InParanoidiP36013.
KOiK00027.
OMAiIRCLDAY.
OrthoDBiEOG092C16UJ.

Enzyme and pathway databases

BioCyciMetaCyc:YKL029C-MONOMER.
YEAST:YKL029C-MONOMER.

Miscellaneous databases

PROiP36013.

Family and domain databases

Gene3Di3.40.50.10380. 1 hit.
3.40.50.720. 1 hit.
InterProiIPR015884. Malic_enzyme_CS.
IPR012301. Malic_N_dom.
IPR012302. Malic_NAD-bd.
IPR001891. Malic_OxRdtase.
IPR016040. NAD(P)-bd_dom.
[Graphical view]
PfamiPF00390. malic. 1 hit.
PF03949. Malic_M. 1 hit.
[Graphical view]
PIRSFiPIRSF000106. ME. 1 hit.
PRINTSiPR00072. MALOXRDTASE.
SMARTiSM01274. malic. 1 hit.
SM00919. Malic_M. 1 hit.
[Graphical view]
SUPFAMiSSF51735. SSF51735. 1 hit.
PROSITEiPS00331. MALIC_ENZYMES. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiMAOM_YEAST
AccessioniPrimary (citable) accession number: P36013
Secondary accession number(s): D6VXQ5
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 1, 1994
Last sequence update: June 1, 1994
Last modified: September 7, 2016
This is version 142 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 10500 molecules/cell in log phase SD medium.1 Publication

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  3. Yeast chromosome XI
    Yeast (Saccharomyces cerevisiae) chromosome XI: entries and gene names

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.