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P36012

- CENPA_YEAST

UniProt

P36012 - CENPA_YEAST

Protein

Histone H3-like centromeric protein CSE4

Gene

CSE4

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 128 (01 Oct 2014)
      Sequence version 2 (01 Nov 1995)
      Previous versions | rss
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    Functioni

    Histone H3-like variant which exclusively replaces conventional H3 in the nucleosome core of centromeric chromatin at the inner plate of the kinetochore. Required for recruitment and assembly of kinetochore proteins, mitotic progression and chromosome segregation. May serve as an epigenetic mark that propagates centromere identity through replication and cell division. Required for functional chromatin architecture at the yeast 2-micron circle partitioning locus and promotes equal plasmid segregation.11 Publications

    GO - Molecular functioni

    1. centromeric DNA binding Source: SGD
    2. protein binding Source: UniProtKB
    3. sequence-specific DNA binding Source: SGD

    GO - Biological processi

    1. 2-micrometer plasmid partitioning Source: SGD
    2. mitotic sister chromatid segregation Source: SGD
    3. nucleosome assembly Source: InterPro

    Keywords - Ligandi

    DNA-binding

    Enzyme and pathway databases

    BioCyciYEAST:G3O-31850-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Histone H3-like centromeric protein CSE4
    Alternative name(s):
    CENP-A homolog
    Chromosome segregation protein 4
    Gene namesi
    Name:CSE4
    Synonyms:CSL2
    Ordered Locus Names:YKL049C
    ORF Names:YKL262
    OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
    Taxonomic identifieri559292 [NCBI]
    Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
    ProteomesiUP000002311: Chromosome XI

    Organism-specific databases

    CYGDiYKL049c.
    SGDiS000001532. CSE4.

    Subcellular locationi

    Nucleus. Chromosomecentromerekinetochore
    Note: Localizes exclusively in the kinetochore domain of centromeres.

    GO - Cellular componenti

    1. CENP-A containing nucleosome Source: SGD
    2. chromosome, centromeric region Source: SGD
    3. condensed chromosome kinetochore Source: UniProtKB-SubCell
    4. condensed nuclear chromosome, centromeric region Source: SGD
    5. extrachromosomal circular DNA Source: SGD
    6. kinetochore Source: SGD

    Keywords - Cellular componenti

    Centromere, Chromosome, Kinetochore, Nucleus

    Pathology & Biotechi

    Mutagenesis

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Mutagenesisi176 – 1761L → S in CSE4-102; impairs nuclear division by disrupting the core centromere structure; when associated with T-218. 1 Publication
    Mutagenesisi194 – 1941L → Q in CSE4-111; impairs nuclear division by disrupting the core centromere structure. 1 Publication
    Mutagenesisi197 – 1971L → S in CSE4-110; impairs nuclear division by disrupting the core centromere structure. 1 Publication
    Mutagenesisi218 – 2181M → T in CSE4-102; impairs nuclear division by disrupting the core centromere structure; when associated with S-176. 1 Publication

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 229229Histone H3-like centromeric protein CSE4PRO_0000221376Add
    BLAST

    Post-translational modificationi

    Ubiquitinated Probable. Is degraded through ubiquitin mediated proteolysis when not protected by its association to the kinetochore. This may ensure exclusive localization of CSE4 to the kinetochore.Curated

    Keywords - PTMi

    Ubl conjugation

    Proteomic databases

    MaxQBiP36012.
    PaxDbiP36012.

    Expressioni

    Gene expression databases

    GenevestigatoriP36012.

    Interactioni

    Subunit structurei

    Component of the core kinetochore. Interacts with the central kinetochore protein CTF19.2 Publications

    Binary interactionsi

    WithEntry#Exp.IntActNotes
    DAM1P532672EBI-5182,EBI-23268

    Protein-protein interaction databases

    BioGridi34084. 353 interactions.
    DIPiDIP-8048N.
    IntActiP36012. 7 interactions.
    MINTiMINT-4492546.
    STRINGi4932.YKL049C.

    Structurei

    Secondary structure

    1
    229
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Helixi157 – 16711
    Helixi171 – 1733
    Helixi180 – 20627
    Turni208 – 2103
    Helixi212 – 2143
    Helixi217 – 2226

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    2FSBmodel-E1-228[»]
    2FSCmodel-A1-228[»]
    2L5ANMR-A151-228[»]
    2LY8NMR-A152-225[»]
    ProteinModelPortaliP36012.
    SMRiP36012. Positions 152-229.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni132 – 22998H3-likeAdd
    BLAST

    Motif

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Motifi115 – 13218Nuclear localization signalSequence AnalysisAdd
    BLAST

    Compositional bias

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Compositional biasi1 – 6666Ser-richAdd
    BLAST

    Sequence similaritiesi

    Belongs to the histone H3 family.Curated

    Phylogenomic databases

    eggNOGiCOG2036.
    GeneTreeiENSGT00730000110760.
    HOGENOMiHOG000155290.
    OMAiRITIMKK.
    OrthoDBiEOG7T4MZ6.

    Family and domain databases

    Gene3Di1.10.20.10. 1 hit.
    InterProiIPR009072. Histone-fold.
    IPR007125. Histone_core_D.
    IPR000164. Histone_H3.
    [Graphical view]
    PANTHERiPTHR11426. PTHR11426. 1 hit.
    PfamiPF00125. Histone. 1 hit.
    [Graphical view]
    PRINTSiPR00622. HISTONEH3.
    SMARTiSM00428. H3. 1 hit.
    [Graphical view]
    SUPFAMiSSF47113. SSF47113. 1 hit.
    PROSITEiPS00959. HISTONE_H3_2. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P36012-1 [UniParc]FASTAAdd to Basket

    « Hide

    MSSKQQWVSS AIQSDSSGRS LSNVNRLAGD QQSINDRALS LLQRTRATKN    50
    LFPRREERRR YESSKSDLDI ETDYEDQAGN LEIETENEEE AEMETEVPAP 100
    VRTHSYALDR YVRQKRREKQ RKQSLKRVEK KYTPSELALY EIRKYQRSTD 150
    LLISKIPFAR LVKEVTDEFT TKDQDLRWQS MAIMALQEAS EAYLVGLLEH 200
    TNLLALHAKR ITIMKKDMQL ARRIRGQFI 229
    Length:229
    Mass (Da):26,841
    Last modified:November 1, 1995 - v2
    Checksum:i0170A10060EEC309
    GO

    Sequence cautioni

    The sequence CAA81884.1 differs from that shown. Reason: Frameshift at position 28.

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U20327 Genomic DNA. Translation: AAB60309.1.
    X71621 Genomic DNA. No translation available.
    Z28049 Genomic DNA. Translation: CAA81884.1. Frameshift.
    BK006944 Genomic DNA. Translation: DAA09108.1.
    PIRiS37870.
    RefSeqiNP_012875.2. NM_001179615.1.

    Genome annotation databases

    EnsemblFungiiYKL049C; YKL049C; YKL049C.
    GeneIDi853817.
    KEGGisce:YKL049C.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U20327 Genomic DNA. Translation: AAB60309.1 .
    X71621 Genomic DNA. No translation available.
    Z28049 Genomic DNA. Translation: CAA81884.1 . Frameshift.
    BK006944 Genomic DNA. Translation: DAA09108.1 .
    PIRi S37870.
    RefSeqi NP_012875.2. NM_001179615.1.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    2FSB model - E 1-228 [» ]
    2FSC model - A 1-228 [» ]
    2L5A NMR - A 151-228 [» ]
    2LY8 NMR - A 152-225 [» ]
    ProteinModelPortali P36012.
    SMRi P36012. Positions 152-229.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 34084. 353 interactions.
    DIPi DIP-8048N.
    IntActi P36012. 7 interactions.
    MINTi MINT-4492546.
    STRINGi 4932.YKL049C.

    Proteomic databases

    MaxQBi P36012.
    PaxDbi P36012.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblFungii YKL049C ; YKL049C ; YKL049C .
    GeneIDi 853817.
    KEGGi sce:YKL049C.

    Organism-specific databases

    CYGDi YKL049c.
    SGDi S000001532. CSE4.

    Phylogenomic databases

    eggNOGi COG2036.
    GeneTreei ENSGT00730000110760.
    HOGENOMi HOG000155290.
    OMAi RITIMKK.
    OrthoDBi EOG7T4MZ6.

    Enzyme and pathway databases

    BioCyci YEAST:G3O-31850-MONOMER.

    Miscellaneous databases

    NextBioi 974993.

    Gene expression databases

    Genevestigatori P36012.

    Family and domain databases

    Gene3Di 1.10.20.10. 1 hit.
    InterProi IPR009072. Histone-fold.
    IPR007125. Histone_core_D.
    IPR000164. Histone_H3.
    [Graphical view ]
    PANTHERi PTHR11426. PTHR11426. 1 hit.
    Pfami PF00125. Histone. 1 hit.
    [Graphical view ]
    PRINTSi PR00622. HISTONEH3.
    SMARTi SM00428. H3. 1 hit.
    [Graphical view ]
    SUPFAMi SSF47113. SSF47113. 1 hit.
    PROSITEi PS00959. HISTONE_H3_2. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "A mutation in CSE4, an essential gene encoding a novel chromatin-associated protein in yeast, causes chromosome nondisjunction and cell cycle arrest at mitosis."
      Stoler S., Keith K.C., Curnick K.E., Fitzgerald-Hayes M.
      Genes Dev. 9:573-586(1995) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION.
    2. "The sequence of a 17.5 kb DNA fragment on the left arm of yeast chromosome XI identifies the protein kinase gene ELM1, the DNA primase gene PRI2, a new gene encoding a putative histone and seven new open reading frames."
      Purnelle B., Tettelin H., van Dyck L., Skala J., Goffeau A.
      Yeast 9:1379-1384(1993) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 204508 / S288c.
    3. Cited for: GENOME REANNOTATION.
      Strain: ATCC 204508 / S288c.
    4. "Complete DNA sequence of yeast chromosome XI."
      Dujon B., Alexandraki D., Andre B., Ansorge W., Baladron V., Ballesta J.P.G., Banrevi A., Bolle P.-A., Bolotin-Fukuhara M., Bossier P., Bou G., Boyer J., Buitrago M.J., Cheret G., Colleaux L., Daignan-Fornier B., del Rey F., Dion C.
      , Domdey H., Duesterhoeft A., Duesterhus S., Entian K.-D., Erfle H., Esteban P.F., Feldmann H., Fernandes L., Fobo G.M., Fritz C., Fukuhara H., Gabel C., Gaillon L., Garcia-Cantalejo J.M., Garcia-Ramirez J.J., Gent M.E., Ghazvini M., Goffeau A., Gonzalez A., Grothues D., Guerreiro P., Hegemann J.H., Hewitt N., Hilger F., Hollenberg C.P., Horaitis O., Indge K.J., Jacquier A., James C.M., Jauniaux J.-C., Jimenez A., Keuchel H., Kirchrath L., Kleine K., Koetter P., Legrain P., Liebl S., Louis E.J., Maia e Silva A., Marck C., Monnier A.-L., Moestl D., Mueller S., Obermaier B., Oliver S.G., Pallier C., Pascolo S., Pfeiffer F., Philippsen P., Planta R.J., Pohl F.M., Pohl T.M., Poehlmann R., Portetelle D., Purnelle B., Puzos V., Ramezani Rad M., Rasmussen S.W., Remacha M.A., Revuelta J.L., Richard G.-F., Rieger M., Rodrigues-Pousada C., Rose M., Rupp T., Santos M.A., Schwager C., Sensen C., Skala J., Soares H., Sor F., Stegemann J., Tettelin H., Thierry A., Tzermia M., Urrestarazu L.A., van Dyck L., van Vliet-Reedijk J.C., Valens M., Vandenbol M., Vilela C., Vissers S., von Wettstein D., Voss H., Wiemann S., Xu G., Zimmermann J., Haasemann M., Becker I., Mewes H.-W.
      Nature 369:371-378(1994) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 204508 / S288c.
    5. "Cse4p is a component of the core centromere of Saccharomyces cerevisiae."
      Meluh P.B., Yang P., Glowczewski L., Koshland D., Smith M.M.
      Cell 94:607-613(1998) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, SUBCELLULAR LOCATION.
    6. "Mutations synthetically lethal with cep1 target S. cerevisiae kinetochore components."
      Baker R.E., Harris K., Zhang K.
      Genetics 149:73-85(1998) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION.
    7. "A putative protein complex consisting of Ctf19, Mcm21, and Okp1 represents a missing link in the budding yeast kinetochore."
      Ortiz J., Stemmann O., Rank S., Lechner J.
      Genes Dev. 13:1140-1155(1999) [PubMed] [Europe PMC] [Abstract]
      Cited for: SUBCELLULAR LOCATION.
    8. "Analysis of primary structural determinants that distinguish the centromere-specific function of histone variant Cse4p from histone H3."
      Keith K.C., Baker R.E., Chen Y., Harris K., Stoler S., Fitzgerald-Hayes M.
      Mol. Cell. Biol. 19:6130-6139(1999) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION.
    9. "CSE4 genetically interacts with the Saccharomyces cerevisiae centromere DNA elements CDE I and CDE II but not CDE III. Implications for the path of the centromere dna around a cse4p variant nucleosome."
      Keith K.C., Fitzgerald-Hayes M.
      Genetics 156:973-981(2000) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, SUBCELLULAR LOCATION.
    10. Cited for: FUNCTION, MUTAGENESIS OF LEU-176; LEU-194; LEU-197 AND MET-218.
    11. "Identification of cohesin association sites at centromeres and along chromosome arms."
      Tanaka T., Cosma M.P., Wirth K., Nasmyth K.
      Cell 98:847-858(1999) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION.
    12. "The N-terminus of the centromere H3-like protein Cse4p performs an essential function distinct from that of the histone fold domain."
      Chen Y., Baker R.E., Keith K.C., Harris K., Stoler S., Fitzgerald-Hayes M.
      Mol. Cell. Biol. 20:7037-7048(2000) [PubMed] [Europe PMC] [Abstract]
      Cited for: INTERACTION WITH CTF19.
    13. "Genes involved in sister chromatid separation and segregation in the budding yeast Saccharomyces cerevisiae."
      Biggins S., Bhalla N., Chang A., Smith D.L., Murray A.W.
      Genetics 159:453-470(2001) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION.
    14. "Budding yeast chromosome structure and dynamics during mitosis."
      Pearson C.G., Maddox P.S., Salmon E.D., Bloom K.S.
      J. Cell Biol. 152:1255-1266(2001) [PubMed] [Europe PMC] [Abstract]
      Cited for: SUBCELLULAR LOCATION.
    15. "Architecture of the budding yeast kinetochore reveals a conserved molecular core."
      Westermann S., Cheeseman I.M., Anderson S., Yates J.R. III, Drubin D.G., Barnes G.
      J. Cell Biol. 163:215-222(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: IDENTIFICATION IN THE CORE KINETOCHORE.
    16. "Proteolysis contributes to the exclusive centromere localization of the yeast Cse4/CENP-A histone H3 variant."
      Collins K.A., Furuyama S., Biggins S.
      Curr. Biol. 14:1968-1972(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: SUBCELLULAR LOCATION, UBIQUITIN-MEDIATED PROTEOLYSIS.
    17. "The histone fold domain of Cse4 is sufficient for CEN targeting and propagation of active centromeres in budding yeast."
      Morey L., Barnes K., Chen Y., Fitzgerald-Hayes M., Baker R.E.
      Eukaryot. Cell 3:1533-1543(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION.
    18. "De novo kinetochore assembly requires the centromeric histone H3 variant."
      Collins K.A., Castillo A.R., Tatsutani S.Y., Biggins S.
      Mol. Biol. Cell 16:5649-5660(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: SUBCELLULAR LOCATION, FUNCTION.
    19. "The centromere-specific histone variant Cse4p (CENP-A) is essential for functional chromatin architecture at the yeast 2-micrometer circle partitioning locus and promotes equal plasmid segregation."
      Hajra S., Ghosh S.K., Jayaram M.
      J. Cell Biol. 174:779-790(2006) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, SUBCELLULAR LOCATION.
    20. "Molecular architecture of a kinetochore-microtubule attachment site."
      Joglekar A.P., Bouck D.C., Molk J.N., Bloom K.S., Salmon E.D.
      Nat. Cell Biol. 8:581-585(2006) [PubMed] [Europe PMC] [Abstract]
      Cited for: SUBCELLULAR LOCATION.
    21. Cited for: 3D-STRUCTURE MODELING OF 1-228.

    Entry informationi

    Entry nameiCENPA_YEAST
    AccessioniPrimary (citable) accession number: P36012
    Secondary accession number(s): D6VXN8
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: June 1, 1994
    Last sequence update: November 1, 1995
    Last modified: October 1, 2014
    This is version 128 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programFungal Protein Annotation Program

    Miscellaneousi

    Miscellaneous

    Mutation in CSE4 causes chromosome non-disjunction and cell cycle arrest at mitosis.

    Keywords - Technical termi

    3D-structure, Complete proteome, Reference proteome

    Documents

    1. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    2. SIMILARITY comments
      Index of protein domains and families
    3. Yeast
      Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
    4. Yeast chromosome XI
      Yeast (Saccharomyces cerevisiae) chromosome XI: entries and gene names

    External Data

    Dasty 3