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Protein

Histone H3-like centromeric protein CSE4

Gene

CSE4

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Histone H3-like variant which exclusively replaces conventional H3 in the nucleosome core of centromeric chromatin at the inner plate of the kinetochore. Required for recruitment and assembly of kinetochore proteins, mitotic progression and chromosome segregation. May serve as an epigenetic mark that propagates centromere identity through replication and cell division. Required for functional chromatin architecture at the yeast 2-micron circle partitioning locus and promotes equal plasmid segregation.11 Publications

Miscellaneous

Mutation in CSE4 causes chromosome non-disjunction and cell cycle arrest at mitosis.

GO - Molecular functioni

  • centromeric DNA binding Source: SGD
  • nucleosomal DNA binding Source: GO_Central
  • protein heterodimerization activity Source: InterPro
  • sequence-specific DNA binding Source: SGD

GO - Biological processi

  • 2-micrometer plasmid partitioning Source: SGD
  • kinetochore assembly Source: SGD
  • mitotic sister chromatid segregation Source: SGD
  • nucleosome assembly Source: GO_Central

Keywordsi

Molecular functionDNA-binding

Enzyme and pathway databases

BioCyciYEAST:G3O-31850-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Histone H3-like centromeric protein CSE4
Alternative name(s):
CENP-A homolog
Chromosome segregation protein 4
Gene namesi
Name:CSE4
Synonyms:CSL2
Ordered Locus Names:YKL049C
ORF Names:YKL262
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome XI

Organism-specific databases

EuPathDBiFungiDB:YKL049C.
SGDiS000001532. CSE4.

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Centromere, Chromosome, Kinetochore, Nucleus

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi176L → S in CSE4-102; impairs nuclear division by disrupting the core centromere structure; when associated with T-218. 1 Publication1
Mutagenesisi194L → Q in CSE4-111; impairs nuclear division by disrupting the core centromere structure. 1 Publication1
Mutagenesisi197L → S in CSE4-110; impairs nuclear division by disrupting the core centromere structure. 1 Publication1
Mutagenesisi218M → T in CSE4-102; impairs nuclear division by disrupting the core centromere structure; when associated with S-176. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002213761 – 229Histone H3-like centromeric protein CSE4Add BLAST229

Post-translational modificationi

Ubiquitinated (Probable). Is degraded through ubiquitin mediated proteolysis when not protected by its association to the kinetochore. This may ensure exclusive localization of CSE4 to the kinetochore.Curated

Keywords - PTMi

Ubl conjugation

Proteomic databases

MaxQBiP36012.

Interactioni

Subunit structurei

Component of the core kinetochore. Interacts with the central kinetochore protein CTF19.2 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
DAM1P532673EBI-5182,EBI-23268

GO - Molecular functioni

Protein-protein interaction databases

BioGridi34084. 397 interactors.
DIPiDIP-8048N.
IntActiP36012. 7 interactors.
MINTiMINT-4492546.
STRINGi4932.YKL049C.

Structurei

Secondary structure

1229
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi157 – 169Combined sources13
Helixi180 – 204Combined sources25
Turni205 – 207Combined sources3
Helixi214 – 225Combined sources12

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2FSBmodel-E1-228[»]
2FSCmodel-A1-228[»]
2L5ANMR-A151-228[»]
2LY8NMR-A152-225[»]
ProteinModelPortaliP36012.
SMRiP36012.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni132 – 229H3-likeAdd BLAST98

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi115 – 132Nuclear localization signalSequence analysisAdd BLAST18

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi1 – 66Ser-richAdd BLAST66

Sequence similaritiesi

Belongs to the histone H3 family.Curated

Phylogenomic databases

GeneTreeiENSGT00840000129844.
HOGENOMiHOG000155290.
InParanoidiP36012.
OMAiKRITIMR.
OrthoDBiEOG092C5B6S.

Family and domain databases

InterProiView protein in InterPro
IPR009072. Histone-fold.
IPR007125. Histone_H2A/H2B/H3.
IPR000164. Histone_H3/CENP-A.
PANTHERiPTHR11426. PTHR11426. 1 hit.
PfamiView protein in Pfam
PF00125. Histone. 1 hit.
PRINTSiPR00622. HISTONEH3.
SMARTiView protein in SMART
SM00428. H3. 1 hit.
SUPFAMiSSF47113. SSF47113. 1 hit.
PROSITEiView protein in PROSITE
PS00959. HISTONE_H3_2. 1 hit.

Sequencei

Sequence statusi: Complete.

P36012-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSSKQQWVSS AIQSDSSGRS LSNVNRLAGD QQSINDRALS LLQRTRATKN
60 70 80 90 100
LFPRREERRR YESSKSDLDI ETDYEDQAGN LEIETENEEE AEMETEVPAP
110 120 130 140 150
VRTHSYALDR YVRQKRREKQ RKQSLKRVEK KYTPSELALY EIRKYQRSTD
160 170 180 190 200
LLISKIPFAR LVKEVTDEFT TKDQDLRWQS MAIMALQEAS EAYLVGLLEH
210 220
TNLLALHAKR ITIMKKDMQL ARRIRGQFI
Length:229
Mass (Da):26,841
Last modified:November 1, 1995 - v2
Checksum:i0170A10060EEC309
GO

Sequence cautioni

The sequence CAA81884 differs from that shown. Reason: Frameshift at position 28.Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U20327 Genomic DNA. Translation: AAB60309.1.
X71621 Genomic DNA. No translation available.
Z28049 Genomic DNA. Translation: CAA81884.1. Frameshift.
BK006944 Genomic DNA. Translation: DAA09108.1.
PIRiS37870.
RefSeqiNP_012875.2. NM_001179615.1.

Genome annotation databases

EnsemblFungiiYKL049C; YKL049C; YKL049C.
GeneIDi853817.
KEGGisce:YKL049C.

Similar proteinsi

Entry informationi

Entry nameiCENPA_YEAST
AccessioniPrimary (citable) accession number: P36012
Secondary accession number(s): D6VXN8
Entry historyiIntegrated into UniProtKB/Swiss-Prot: June 1, 1994
Last sequence update: November 1, 1995
Last modified: September 27, 2017
This is version 151 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families
  3. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  4. Yeast chromosome XI
    Yeast (Saccharomyces cerevisiae) chromosome XI: entries and gene names