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P36012 (CENPA_YEAST) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 127. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (4) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Histone H3-like centromeric protein CSE4
Alternative name(s):
CENP-A homolog
Chromosome segregation protein 4
Gene names
Name:CSE4
Synonyms:CSL2
Ordered Locus Names:YKL049C
ORF Names:YKL262
OrganismSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]
Taxonomic identifier559292 [NCBI]
Taxonomic lineageEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces

Protein attributes

Sequence length229 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Histone H3-like variant which exclusively replaces conventional H3 in the nucleosome core of centromeric chromatin at the inner plate of the kinetochore. Required for recruitment and assembly of kinetochore proteins, mitotic progression and chromosome segregation. May serve as an epigenetic mark that propagates centromere identity through replication and cell division. Required for functional chromatin architecture at the yeast 2-micron circle partitioning locus and promotes equal plasmid segregation. Ref.1 Ref.5 Ref.6 Ref.8 Ref.9 Ref.10 Ref.11 Ref.13 Ref.17 Ref.18 Ref.19

Subunit structure

Component of the core kinetochore. Interacts with the central kinetochore protein CTF19. Ref.12 Ref.15

Subcellular location

Nucleus. Chromosomecentromerekinetochore. Note: Localizes exclusively in the kinetochore domain of centromeres. Ref.5 Ref.7 Ref.9 Ref.14 Ref.16 Ref.18 Ref.19 Ref.20

Post-translational modification

Ubiquitinated Probable. Is degraded through ubiquitin mediated proteolysis when not protected by its association to the kinetochore. This may ensure exclusive localization of CSE4 to the kinetochore.

Miscellaneous

Mutation in CSE4 causes chromosome non-disjunction and cell cycle arrest at mitosis.

Sequence similarities

Belongs to the histone H3 family.

Sequence caution

The sequence CAA81884.1 differs from that shown. Reason: Frameshift at position 28.

Binary interactions

With

Entry

#Exp.

IntAct

Notes

DAM1P532672EBI-5182,EBI-23268

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 229229Histone H3-like centromeric protein CSE4
PRO_0000221376

Regions

Region132 – 22998H3-like
Motif115 – 13218Nuclear localization signal Potential
Compositional bias1 – 6666Ser-rich

Experimental info

Mutagenesis1761L → S in CSE4-102; impairs nuclear division by disrupting the core centromere structure; when associated with T-218. Ref.10
Mutagenesis1941L → Q in CSE4-111; impairs nuclear division by disrupting the core centromere structure. Ref.10
Mutagenesis1971L → S in CSE4-110; impairs nuclear division by disrupting the core centromere structure. Ref.10
Mutagenesis2181M → T in CSE4-102; impairs nuclear division by disrupting the core centromere structure; when associated with S-176. Ref.10

Secondary structure

............. 229
Helix Strand Turn

Details...

Sequences

Sequence LengthMass (Da)Tools
P36012 [UniParc].

Last modified November 1, 1995. Version 2.
Checksum: 0170A10060EEC309

FASTA22926,841
        10         20         30         40         50         60 
MSSKQQWVSS AIQSDSSGRS LSNVNRLAGD QQSINDRALS LLQRTRATKN LFPRREERRR 

        70         80         90        100        110        120 
YESSKSDLDI ETDYEDQAGN LEIETENEEE AEMETEVPAP VRTHSYALDR YVRQKRREKQ 

       130        140        150        160        170        180 
RKQSLKRVEK KYTPSELALY EIRKYQRSTD LLISKIPFAR LVKEVTDEFT TKDQDLRWQS 

       190        200        210        220 
MAIMALQEAS EAYLVGLLEH TNLLALHAKR ITIMKKDMQL ARRIRGQFI 

« Hide

References

« Hide 'large scale' references
[1]"A mutation in CSE4, an essential gene encoding a novel chromatin-associated protein in yeast, causes chromosome nondisjunction and cell cycle arrest at mitosis."
Stoler S., Keith K.C., Curnick K.E., Fitzgerald-Hayes M.
Genes Dev. 9:573-586(1995) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION.
[2]"The sequence of a 17.5 kb DNA fragment on the left arm of yeast chromosome XI identifies the protein kinase gene ELM1, the DNA primase gene PRI2, a new gene encoding a putative histone and seven new open reading frames."
Purnelle B., Tettelin H., van Dyck L., Skala J., Goffeau A.
Yeast 9:1379-1384(1993) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 204508 / S288c.
[3]"The reference genome sequence of Saccharomyces cerevisiae: Then and now."
Engel S.R., Dietrich F.S., Fisk D.G., Binkley G., Balakrishnan R., Costanzo M.C., Dwight S.S., Hitz B.C., Karra K., Nash R.S., Weng S., Wong E.D., Lloyd P., Skrzypek M.S., Miyasato S.R., Simison M., Cherry J.M.
G3 (Bethesda) 4:389-398(2014) [PubMed] [Europe PMC] [Abstract]
Cited for: GENOME REANNOTATION.
Strain: ATCC 204508 / S288c.
[4]"Complete DNA sequence of yeast chromosome XI."
Dujon B., Alexandraki D., Andre B., Ansorge W., Baladron V., Ballesta J.P.G., Banrevi A., Bolle P.-A., Bolotin-Fukuhara M., Bossier P., Bou G., Boyer J., Buitrago M.J., Cheret G., Colleaux L., Daignan-Fornier B., del Rey F., Dion C. expand/collapse author list , Domdey H., Duesterhoeft A., Duesterhus S., Entian K.-D., Erfle H., Esteban P.F., Feldmann H., Fernandes L., Fobo G.M., Fritz C., Fukuhara H., Gabel C., Gaillon L., Garcia-Cantalejo J.M., Garcia-Ramirez J.J., Gent M.E., Ghazvini M., Goffeau A., Gonzalez A., Grothues D., Guerreiro P., Hegemann J.H., Hewitt N., Hilger F., Hollenberg C.P., Horaitis O., Indge K.J., Jacquier A., James C.M., Jauniaux J.-C., Jimenez A., Keuchel H., Kirchrath L., Kleine K., Koetter P., Legrain P., Liebl S., Louis E.J., Maia e Silva A., Marck C., Monnier A.-L., Moestl D., Mueller S., Obermaier B., Oliver S.G., Pallier C., Pascolo S., Pfeiffer F., Philippsen P., Planta R.J., Pohl F.M., Pohl T.M., Poehlmann R., Portetelle D., Purnelle B., Puzos V., Ramezani Rad M., Rasmussen S.W., Remacha M.A., Revuelta J.L., Richard G.-F., Rieger M., Rodrigues-Pousada C., Rose M., Rupp T., Santos M.A., Schwager C., Sensen C., Skala J., Soares H., Sor F., Stegemann J., Tettelin H., Thierry A., Tzermia M., Urrestarazu L.A., van Dyck L., van Vliet-Reedijk J.C., Valens M., Vandenbol M., Vilela C., Vissers S., von Wettstein D., Voss H., Wiemann S., Xu G., Zimmermann J., Haasemann M., Becker I., Mewes H.-W.
Nature 369:371-378(1994) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 204508 / S288c.
[5]"Cse4p is a component of the core centromere of Saccharomyces cerevisiae."
Meluh P.B., Yang P., Glowczewski L., Koshland D., Smith M.M.
Cell 94:607-613(1998) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, SUBCELLULAR LOCATION.
[6]"Mutations synthetically lethal with cep1 target S. cerevisiae kinetochore components."
Baker R.E., Harris K., Zhang K.
Genetics 149:73-85(1998) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION.
[7]"A putative protein complex consisting of Ctf19, Mcm21, and Okp1 represents a missing link in the budding yeast kinetochore."
Ortiz J., Stemmann O., Rank S., Lechner J.
Genes Dev. 13:1140-1155(1999) [PubMed] [Europe PMC] [Abstract]
Cited for: SUBCELLULAR LOCATION.
[8]"Analysis of primary structural determinants that distinguish the centromere-specific function of histone variant Cse4p from histone H3."
Keith K.C., Baker R.E., Chen Y., Harris K., Stoler S., Fitzgerald-Hayes M.
Mol. Cell. Biol. 19:6130-6139(1999) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION.
[9]"CSE4 genetically interacts with the Saccharomyces cerevisiae centromere DNA elements CDE I and CDE II but not CDE III. Implications for the path of the centromere dna around a cse4p variant nucleosome."
Keith K.C., Fitzgerald-Hayes M.
Genetics 156:973-981(2000) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, SUBCELLULAR LOCATION.
[10]"Histone-histone interactions and centromere function."
Glowczewski L., Yang P., Kalashnikova T., Santisteban M.S., Smith M.M.
Mol. Cell. Biol. 20:5700-5711(2000) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, MUTAGENESIS OF LEU-176; LEU-194; LEU-197 AND MET-218.
[11]"Identification of cohesin association sites at centromeres and along chromosome arms."
Tanaka T., Cosma M.P., Wirth K., Nasmyth K.
Cell 98:847-858(1999) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION.
[12]"The N-terminus of the centromere H3-like protein Cse4p performs an essential function distinct from that of the histone fold domain."
Chen Y., Baker R.E., Keith K.C., Harris K., Stoler S., Fitzgerald-Hayes M.
Mol. Cell. Biol. 20:7037-7048(2000) [PubMed] [Europe PMC] [Abstract]
Cited for: INTERACTION WITH CTF19.
[13]"Genes involved in sister chromatid separation and segregation in the budding yeast Saccharomyces cerevisiae."
Biggins S., Bhalla N., Chang A., Smith D.L., Murray A.W.
Genetics 159:453-470(2001) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION.
[14]"Budding yeast chromosome structure and dynamics during mitosis."
Pearson C.G., Maddox P.S., Salmon E.D., Bloom K.S.
J. Cell Biol. 152:1255-1266(2001) [PubMed] [Europe PMC] [Abstract]
Cited for: SUBCELLULAR LOCATION.
[15]"Architecture of the budding yeast kinetochore reveals a conserved molecular core."
Westermann S., Cheeseman I.M., Anderson S., Yates J.R. III, Drubin D.G., Barnes G.
J. Cell Biol. 163:215-222(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION IN THE CORE KINETOCHORE.
[16]"Proteolysis contributes to the exclusive centromere localization of the yeast Cse4/CENP-A histone H3 variant."
Collins K.A., Furuyama S., Biggins S.
Curr. Biol. 14:1968-1972(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: SUBCELLULAR LOCATION, UBIQUITIN-MEDIATED PROTEOLYSIS.
[17]"The histone fold domain of Cse4 is sufficient for CEN targeting and propagation of active centromeres in budding yeast."
Morey L., Barnes K., Chen Y., Fitzgerald-Hayes M., Baker R.E.
Eukaryot. Cell 3:1533-1543(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION.
[18]"De novo kinetochore assembly requires the centromeric histone H3 variant."
Collins K.A., Castillo A.R., Tatsutani S.Y., Biggins S.
Mol. Biol. Cell 16:5649-5660(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: SUBCELLULAR LOCATION, FUNCTION.
[19]"The centromere-specific histone variant Cse4p (CENP-A) is essential for functional chromatin architecture at the yeast 2-micrometer circle partitioning locus and promotes equal plasmid segregation."
Hajra S., Ghosh S.K., Jayaram M.
J. Cell Biol. 174:779-790(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, SUBCELLULAR LOCATION.
[20]"Molecular architecture of a kinetochore-microtubule attachment site."
Joglekar A.P., Bouck D.C., Molk J.N., Bloom K.S., Salmon E.D.
Nat. Cell Biol. 8:581-585(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: SUBCELLULAR LOCATION.
[21]"The path of DNA in the kinetochore."
Bloom K.S., Sharma S., Dokholyan N.V.
Curr. Biol. 16:R276-R278(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: 3D-STRUCTURE MODELING OF 1-228.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
U20327 Genomic DNA. Translation: AAB60309.1.
X71621 Genomic DNA. No translation available.
Z28049 Genomic DNA. Translation: CAA81884.1. Frameshift.
BK006944 Genomic DNA. Translation: DAA09108.1.
PIRS37870.
RefSeqNP_012875.2. NM_001179615.1.

3D structure databases

PDBe
RCSB-PDB
PDBj
EntryMethodResolution (Å)ChainPositionsPDBsum
2FSBmodel-E1-228[»]
2FSCmodel-A1-228[»]
2L5ANMR-A151-228[»]
2LY8NMR-A152-225[»]
ProteinModelPortalP36012.
SMRP36012. Positions 152-229.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid34084. 353 interactions.
DIPDIP-8048N.
IntActP36012. 7 interactions.
MINTMINT-4492546.
STRING4932.YKL049C.

Proteomic databases

MaxQBP36012.
PaxDbP36012.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblFungiYKL049C; YKL049C; YKL049C.
GeneID853817.
KEGGsce:YKL049C.

Organism-specific databases

CYGDYKL049c.
SGDS000001532. CSE4.

Phylogenomic databases

eggNOGCOG2036.
GeneTreeENSGT00730000110760.
HOGENOMHOG000155290.
OMARITIMKK.
OrthoDBEOG7T4MZ6.

Enzyme and pathway databases

BioCycYEAST:G3O-31850-MONOMER.

Gene expression databases

GenevestigatorP36012.

Family and domain databases

Gene3D1.10.20.10. 1 hit.
InterProIPR009072. Histone-fold.
IPR007125. Histone_core_D.
IPR000164. Histone_H3.
[Graphical view]
PANTHERPTHR11426. PTHR11426. 1 hit.
PfamPF00125. Histone. 1 hit.
[Graphical view]
PRINTSPR00622. HISTONEH3.
SMARTSM00428. H3. 1 hit.
[Graphical view]
SUPFAMSSF47113. SSF47113. 1 hit.
PROSITEPS00959. HISTONE_H3_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio974993.

Entry information

Entry nameCENPA_YEAST
AccessionPrimary (citable) accession number: P36012
Secondary accession number(s): D6VXN8
Entry history
Integrated into UniProtKB/Swiss-Prot: June 1, 1994
Last sequence update: November 1, 1995
Last modified: July 9, 2014
This is version 127 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Relevant documents

Yeast chromosome XI

Yeast (Saccharomyces cerevisiae) chromosome XI: entries and gene names

Yeast

Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD

SIMILARITY comments

Index of protein domains and families

PDB cross-references

Index of Protein Data Bank (PDB) cross-references