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Protein

Cell pattern formation-associated protein stuA

Gene

stuA

Organism
Emericella nidulans (strain FGSC A4 / ATCC 38163 / CBS 112.46 / NRRL 194 / M139) (Aspergillus nidulans)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Transcription factor that regulates asexual reproduction (PubMed:1516832, PubMed:8722771, PubMed:9312029, PubMed:25550299). Binds the StuA-response elements (StRE) with the consensus sequence 5'-(A/T)CGCG(T/A)N(A/C)-3' at the promoters of target genes (PubMed:9312029, PubMed:25359270). Required from the very earliest events of asexual reproduction until completion of conidiophore development, but is not specifically required for differentiation of conidia (PubMed:1516832). Represses transcription of the abaA developmental regulatory gene and of the developmentally regulated awh11 gene (PubMed:9312029). Controls the expression of the catalase-peroxidase gene cpeA (PubMed:12455692). Plays an important role in cell wall biogenesis during the development by controlling the transcription level of fksA (PubMed:25359270).6 Publications

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
DNA bindingi163 – 18422H-T-H motifPROSITE-ProRule annotationAdd
BLAST

GO - Molecular functioni

  • DNA binding Source: UniProtKB-KW
  • transcription factor activity, sequence-specific DNA binding Source: ASPGD

GO - Biological processi

  • conidiophore development Source: ASPGD
  • conidium formation Source: ASPGD
  • positive regulation of conidiophore development Source: ASPGD
  • positive regulation of conidium formation Source: ASPGD
  • regulation of transcription from RNA polymerase II promoter Source: ASPGD
  • sporocarp development involved in asexual reproduction Source: ASPGD
  • sporulation resulting in formation of a cellular spore Source: UniProtKB-KW
  • transcription, DNA-templated Source: UniProtKB-KW
Complete GO annotation...

Keywords - Biological processi

Conidiation, Sporulation, Transcription, Transcription regulation

Keywords - Ligandi

DNA-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Cell pattern formation-associated protein stuACurated
Alternative name(s):
Stunted protein A1 Publication
Gene namesi
Name:stuA1 Publication
ORF Names:AN5836
OrganismiEmericella nidulans (strain FGSC A4 / ATCC 38163 / CBS 112.46 / NRRL 194 / M139) (Aspergillus nidulans)
Taxonomic identifieri227321 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaPezizomycotinaEurotiomycetesEurotiomycetidaeEurotialesAspergillaceaeAspergillus
Proteomesi
  • UP000000560 Componenti: Chromosome I
  • UP000005890 Componenti: Partially assembled WGS sequence

Organism-specific databases

EuPathDBiFungiDB:AN5836.

Subcellular locationi

GO - Cellular componenti

  • nucleus Source: ASPGD
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

Pathology & Biotechi

Disruption phenotypei

Leads to defective conidiophore morphology and impairs conidia production (PubMed:25550299). Affects cell wall beta-1,3-glucan biosynthesis (PubMed:25359270).2 Publications

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 622622Cell pattern formation-associated protein stuAPRO_0000072296Add
BLAST

Expressioni

Inductioni

Expression is increased 50-fold during the establishment of developmental competence (PubMed:1516832, PubMed:2062309, PubMed:17630328). Expression is positively controlled by brlA (PubMed:1516832). Expression is also regulated by the LAMMER kinase lkhA (PubMed:23516554).3 Publications

Structurei

3D structure databases

ProteinModelPortaliP36011.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini129 – 235107HTH APSES-typePROSITE-ProRule annotationAdd
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni565 – 59026Nuclear localization domain1 PublicationAdd
BLAST

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi39 – 12991Gln/Pro/Ser-richAdd
BLAST
Compositional biasi253 – 415163Gln/Pro/Ser-richAdd
BLAST

Sequence similaritiesi

Belongs to the EFG1/PHD1/stuA family.Curated
Contains 1 HTH APSES-type DNA-binding domain.PROSITE-ProRule annotation

Phylogenomic databases

HOGENOMiHOG000213682.
InParanoidiP36011.
KOiK12763.
OMAiYHPTNQT.
OrthoDBiEOG7K9KD5.

Family and domain databases

Gene3Di3.10.260.10. 1 hit.
InterProiIPR018004. KilA_N/APSES_HTH.
IPR003163. Tscrpt_reg_HTH_APSES-type.
[Graphical view]
PfamiPF04383. KilA-N. 1 hit.
[Graphical view]
SMARTiSM01252. KilA-N. 1 hit.
[Graphical view]
SUPFAMiSSF54616. SSF54616. 1 hit.
PROSITEiPS51299. HTH_APSES. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P36011-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MASMNQPQPY MDVHSHLSSG QTYASHPATA GALTHYQYPQ QPPVLQPTST
60 70 80 90 100
YGPASSYSQY PYPNSVASSQ SVPPPTTSIS SQVPAQLLPL PVTNHPVPTH
110 120 130 140 150
GYGNNSGTPM QGYVYDPTGQ MAPPGAKPRV TATLWEDEGS LCYQVEAKGV
160 170 180 190 200
CVARREDNGM INGTKLLNVA GMTRGRRDGI LKSEKVRNVV KIGPMHLKGV
210 220 230 240 250
WIPFDRALEF ANKEKITDLL YPLFVQHISN LLYHPANQNQ RNMTVPDSRR
260 270 280 290 300
LEGPQPVVRT PQAQQPPSLH HHSLQTPVPS HMSQPGGRPS LDRAHTFPTP
310 320 330 340 350
PASASSLIGI TSQNNSYDWN PGMNSSVPNT QPLSIDTSLS NARSMPTTPA
360 370 380 390 400
TTPPGNNLQG MQSYQPQSGY DSKPYYSAAP STHPQYAPQQ PLPQQSMAQY
410 420 430 440 450
GHSMPTSSYR DMAPPSSQRG SVTEIESDVK TERYGQGTVA KTEPEQEQEY
460 470 480 490 500
AQPDSGYNTG RGSYYTTNPS VGGLAHDHSQ LTPDMTGSPQ QNGSGRMTPR
510 520 530 540 550
TSNTAPQWAP GYTTPPRPAA ASSLYNIVSD TRGTSGANGS TSDNYSVASN
560 570 580 590 600
SGYSTGMNGS MGSNKRMRDD DDDRIVPPDS RGEFDTKRRK TLTETPVGGP
610 620
VGGVPLGLQP MKAGGSLISA RR
Length:622
Mass (Da):66,956
Last modified:March 15, 2004 - v2
Checksum:i05D8AE2FAF497083
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M83569 Genomic DNA. Translation: AAA33325.2.
AACD01000100 Genomic DNA. Translation: EAA58345.1.
BN001301 Genomic DNA. Translation: CBF70741.1.
PIRiA44068.
RefSeqiXP_663440.1. XM_658348.1.

Genome annotation databases

EnsemblFungiiCADANIAT00007206; CADANIAP00007206; CADANIAG00007206.
EAA58345; EAA58345; AN5836.2.
GeneIDi2870743.
KEGGiani:AN5836.2.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M83569 Genomic DNA. Translation: AAA33325.2.
AACD01000100 Genomic DNA. Translation: EAA58345.1.
BN001301 Genomic DNA. Translation: CBF70741.1.
PIRiA44068.
RefSeqiXP_663440.1. XM_658348.1.

3D structure databases

ProteinModelPortaliP36011.
ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiCADANIAT00007206; CADANIAP00007206; CADANIAG00007206.
EAA58345; EAA58345; AN5836.2.
GeneIDi2870743.
KEGGiani:AN5836.2.

Organism-specific databases

EuPathDBiFungiDB:AN5836.

Phylogenomic databases

HOGENOMiHOG000213682.
InParanoidiP36011.
KOiK12763.
OMAiYHPTNQT.
OrthoDBiEOG7K9KD5.

Family and domain databases

Gene3Di3.10.260.10. 1 hit.
InterProiIPR018004. KilA_N/APSES_HTH.
IPR003163. Tscrpt_reg_HTH_APSES-type.
[Graphical view]
PfamiPF04383. KilA-N. 1 hit.
[Graphical view]
SMARTiSM01252. KilA-N. 1 hit.
[Graphical view]
SUPFAMiSSF54616. SSF54616. 1 hit.
PROSITEiPS51299. HTH_APSES. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "StuA is required for cell pattern formation in Aspergillus."
    Miller K.Y., Wu J., Miller B.L.
    Genes Dev. 6:1770-1782(1992) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION, INDUCTION.
  2. Miller B.L.
    Submitted (AUG-2003) to the EMBL/GenBank/DDBJ databases
    Cited for: SEQUENCE REVISION.
  3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: FGSC A4 / ATCC 38163 / CBS 112.46 / NRRL 194 / M139.
  4. "The 2008 update of the Aspergillus nidulans genome annotation: a community effort."
    Wortman J.R., Gilsenan J.M., Joardar V., Deegan J., Clutterbuck J., Andersen M.R., Archer D., Bencina M., Braus G., Coutinho P., von Dohren H., Doonan J., Driessen A.J., Durek P., Espeso E., Fekete E., Flipphi M., Estrada C.G.
    , Geysens S., Goldman G., de Groot P.W., Hansen K., Harris S.D., Heinekamp T., Helmstaedt K., Henrissat B., Hofmann G., Homan T., Horio T., Horiuchi H., James S., Jones M., Karaffa L., Karanyi Z., Kato M., Keller N., Kelly D.E., Kiel J.A., Kim J.M., van der Klei I.J., Klis F.M., Kovalchuk A., Krasevec N., Kubicek C.P., Liu B., Maccabe A., Meyer V., Mirabito P., Miskei M., Mos M., Mullins J., Nelson D.R., Nielsen J., Oakley B.R., Osmani S.A., Pakula T., Paszewski A., Paulsen I., Pilsyk S., Pocsi I., Punt P.J., Ram A.F., Ren Q., Robellet X., Robson G., Seiboth B., van Solingen P., Specht T., Sun J., Taheri-Talesh N., Takeshita N., Ussery D., vanKuyk P.A., Visser H., van de Vondervoort P.J., de Vries R.P., Walton J., Xiang X., Xiong Y., Zeng A.P., Brandt B.W., Cornell M.J., van den Hondel C.A., Visser J., Oliver S.G., Turner G.
    Fungal Genet. Biol. 46:S2-13(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: GENOME REANNOTATION.
    Strain: FGSC A4 / ATCC 38163 / CBS 112.46 / NRRL 194 / M139.
  5. "Isolation and transcriptional characterization of a morphological modifier: the Aspergillus nidulans stunted (stuA) gene."
    Miller K.Y., Toennis T.M., Adams T.H., Miller B.L.
    Mol. Gen. Genet. 227:285-292(1991) [PubMed] [Europe PMC] [Abstract]
    Cited for: INDUCTION.
  6. "Suppression and enhancement of the Aspergillus nidulans medusa mutation by altered dosage of the bristle and stunted genes."
    Busby T.M., Miller K.Y., Miller B.L.
    Genetics 143:155-163(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  7. "StuAp is a sequence-specific transcription factor that regulates developmental complexity in Aspergillus nidulans."
    Dutton J.R., Johns S., Miller B.L.
    EMBO J. 16:5710-5721(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, DNA-BINDING.
  8. "Aspergillus asexual reproduction and sexual reproduction are differentially affected by transcriptional and translational mechanisms regulating stunted gene expression."
    Wu J., Miller B.L.
    Mol. Cell. Biol. 17:6191-6201(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: INDUCTION, FUNCTION.
  9. "Nuclear traffic in fungal hyphae: in vivo study of nuclear migration and positioning in Aspergillus nidulans."
    Suelmann R., Sievers N., Fischer R.
    Mol. Microbiol. 25:757-769(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: SUBCELLULAR LOCATION.
  10. "Aspergillus nidulans catalase-peroxidase gene (cpeA) is transcriptionally induced during sexual development through the transcription factor StuA."
    Scherer M., Wei H., Liese R., Fischer R.
    Eukaryot. Cell 1:725-735(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  11. "Aspergillus nidulans conidiation genes dewA, fluG, and stuA are differentially regulated in early vegetative growth."
    Breakspear A., Momany M.
    Eukaryot. Cell 6:1697-1700(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: INDUCTION.
  12. "LAMMER Kinase LkhA plays multiple roles in the vegetative growth and asexual and sexual development of Aspergillus nidulans."
    Kang E.H., Kim J.A., Oh H.W., Park H.M.
    PLoS ONE 8:E58762-E58762(2013) [PubMed] [Europe PMC] [Abstract]
    Cited for: INDUCTION.
  13. "Transcriptional regulation of fksA, a beta-1,3-glucan synthase gene, by the APSES protein StuA during Aspergillus nidulans development."
    Park B.C., Park Y.H., Yi S., Choi Y.K., Kang E.H., Park H.M.
    J. Microbiol. 52:940-947(2014) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, DISRUPTION PHENOTYPE, DNA-BINDING.
  14. "Neurospora crassa ASM-1 complements the conidiation defect in a stuA mutant of Aspergillus nidulans."
    Chung D., Upadhyay S., Bomer B., Wilkinson H.H., Ebbole D.J., Shaw B.D.
    Mycologia 107:298-306(2015) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, DISRUPTION PHENOTYPE, SUBCELLULAR LOCATION.

Entry informationi

Entry nameiSTUA_EMENI
AccessioniPrimary (citable) accession number: P36011
Secondary accession number(s): C8V024, Q5B0U4
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 1, 1994
Last sequence update: March 15, 2004
Last modified: May 11, 2016
This is version 89 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.