P36009 (DHR2_YEAST) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Probable ATP-dependent RNA helicase DHR2
EC=3.6.4.13

Alternative name(s):
DEAH box RNA helicase DHR2
Helicase JA2

Gene names

Name:	DHR2
Ordered Locus Names:	YKL078W
ORF Names:	YKL408

Organism

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]

Taxonomic identifier

559292 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Fungi › Dikarya › Ascomycota › saccharomyceta › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces › Saccharomyces cerevisiae

Protein attributes

Sequence length	735 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Probable ATP-binding RNA helicase. Required for 18S rRNA synthesis.
Catalytic activity	ATP + H2O = ADP + phosphate.
Subunit structure	Interacts with NOP19. Ref.8
Subcellular location	Nucleus › nucleolus.
Miscellaneous	Present with 721 molecules/cell in log phase SD medium.
Sequence similarities	Belongs to the DEAD box helicase family. DEAH subfamily. Contains 1 helicase ATP-binding domain. Contains 1 helicase C-terminal domain.

Ontologies

Keywords
Biological process	rRNA processing
Cellular component	Nucleus
Ligand	ATP-binding Nucleotide-binding RNA-binding
Molecular function	Helicase Hydrolase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	maturation of SSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) Inferred from mutant phenotype PubMed 16449634. Source: SGD
Cellular_component	nucleolus Inferred from direct assay Ref.6. Source: SGD
Molecular_function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW ATP-dependent helicase activity Inferred from electronic annotation. Source: InterPro RNA binding Inferred from electronic annotation. Source: UniProtKB-KW RNA helicase activity Inferred from direct assay Ref.6. Source: SGD
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 735	735	Probable ATP-dependent RNA helicase DHR2		PRO_0000055163
Regions
	Domain	91 – 257	167	Helicase ATP-binding
	Domain	262 – 456	195	Helicase C-terminal
	Nucleotide binding	104 – 111	8	ATP By similarity
	Motif	203 – 206	4	DEAH box
Experimental info
	Sequence conflict	28	1	T → M in AAA34986. Ref.2
	Sequence conflict	80 – 98	19	TLPVY…IESNP → HFRLPTQARNNVIYSKQS in AAA34986. Ref.2
	Sequence conflict	277 – 288	12	DAVIR…IQINQ → ALSSGVYTNKP in AAA34986. Ref.2
	Sequence conflict	339	1	A → P in AAA34986. Ref.2

Sequences

	Sequence		Length	Mass (Da)
	P36009 [UniParc]. Last modified June 1, 1994. Version 1. Checksum: AAFA78B5F6215D89	FASTA	735	82,713
	10 20 30 40 50 60 MAANSNSRVA SNHTSKKQKV RRNIHPFTNN TRIKRASKIV KFNDSGEGDH VSDQRSNKEN 70 80 90 100 110 120 VLTYKSLKSR ASDLLKMRET LPVYQHKREI MSYIESNPVT VLIGETGSGK STQIPQFVLE 130 140 150 160 170 180 KLYDTKKHGS IAVTQPRRVA AINLATRVAQ EHGCKLGEQV GYSVRFDNTT TTRTRLKYLT 190 200 210 220 230 240 DGMLLRELMM NSDLREYSVI VIDEAHERTV LTDLILGFLK SLIQGPRPDL RIIVMSATLQ 250 260 270 280 290 300 AEKFSEFFNN APILFVEGRK FDVKQYYLKA PTDDIVDAVI RCCIQINQGE ELGDILCFLP 310 320 330 340 350 360 GQEEIDKAVT IMEKIAKYVS DEAPVPLIVP YPLYAALPAV QQSLVFAPIK GFKRKVVFST 370 380 390 400 410 420 NIAETSVTIS GVKFVVDSGL RKVKVWRHQL GLATLLTVPI SQASAMQRSG RAGRESEGKS 430 440 450 460 470 480 FRLYCESDYV KLPKQSEPEI ARSDVTSPVL MLKRYGVDDL LNWTWFENPG KEAIVMGLQE 490 500 510 520 530 540 LYELGALDTR GKITKRGQQM ALLPLQPHLS SVLIKASEVG CLSQVIDIVS CLSVENLLLN 550 560 570 580 590 600 PSPEERDEVN ERRLSLCNAG KRYGDLIMLK ELFDIYFYEL GKSQDASSER NDWCKGLCIS 610 620 630 640 650 660 IRGFKNVIRV RDQLRVYCKR LFSSISEEDE ESKKIGEDGE LISKILKCFL TGFIKNTAIG 670 680 690 700 710 720 MPDRSYRTVS TGEPISIHPS SMLFMNKSCP GIMYTEYVFT TKGYARNVSR IELSWLQEVV 730 TNAAAVAKQK VSDSK

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"JA2: a DEAH family RNA-helicase-like gene from Saccharomyces cerevisiae." Arenas J.E., Messenger J. Submitted (JUL-1997) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"A clone isolated from a Clontech HeLa cDNA library has high homology to yeast DEAH helicases and hybridizes to Northern and Southern blots from yeast but not from HeLa." Raju V.S., Datta P.K., Beales M.H., Ghoshal K., Jacob S.T. Submitted (NOV-1993) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[3]	"Sequence analysis of a 10 kb fragment of yeast chromosome XI identifies the SMY1 locus and reveals sequences related to a pre-mRNA splicing factor and vacuolar ATPase subunit C plus a number of unidentified open reading frames." James C.M., Gent M.E., Indge K.J., Oliver S.G. Yeast 10:247-255(1994) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[4]	"Complete DNA sequence of yeast chromosome XI." Dujon B., Alexandraki D., Andre B., Ansorge W., Baladron V., Ballesta J.P.G., Banrevi A., Bolle P.-A., Bolotin-Fukuhara M., Bossier P., Bou G., Boyer J., Buitrago M.J., Cheret G., Colleaux L., Daignan-Fornier B., del Rey F., Dion C. , Domdey H., Duesterhoeft A., Duesterhus S., Entian K.-D., Erfle H., Esteban P.F., Feldmann H., Fernandes L., Fobo G.M., Fritz C., Fukuhara H., Gabel C., Gaillon L., Garcia-Cantalejo J.M., Garcia-Ramirez J.J., Gent M.E., Ghazvini M., Goffeau A., Gonzalez A., Grothues D., Guerreiro P., Hegemann J.H., Hewitt N., Hilger F., Hollenberg C.P., Horaitis O., Indge K.J., Jacquier A., James C.M., Jauniaux J.-C., Jimenez A., Keuchel H., Kirchrath L., Kleine K., Koetter P., Legrain P., Liebl S., Louis E.J., Maia e Silva A., Marck C., Monnier A.-L., Moestl D., Mueller S., Obermaier B., Oliver S.G., Pallier C., Pascolo S., Pfeiffer F., Philippsen P., Planta R.J., Pohl F.M., Pohl T.M., Poehlmann R., Portetelle D., Purnelle B., Puzos V., Ramezani Rad M., Rasmussen S.W., Remacha M.A., Revuelta J.L., Richard G.-F., Rieger M., Rodrigues-Pousada C., Rose M., Rupp T., Santos M.A., Schwager C., Sensen C., Skala J., Soares H., Sor F., Stegemann J., Tettelin H., Thierry A., Tzermia M., Urrestarazu L.A., van Dyck L., van Vliet-Reedijk J.C., Valens M., Vandenbol M., Vilela C., Vissers S., von Wettstein D., Voss H., Wiemann S., Xu G., Zimmermann J., Haasemann M., Becker I., Mewes H.-W. Nature 369:371-378(1994) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 96604 / S288c / FY1679.
[5]	Saccharomyces Genome Database Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: ATCC 204508 / S288c.
[6]	"Dhr1p, a putative DEAH-Box RNA helicase, is associated with the box C+D snoRNP U3." Colley A., Beggs J.D., Tollervey D., Lafontaine D.L.J. Mol. Cell. Biol. 20:7238-7246(2000) [PubMed] [Europe PMC] [Abstract] Cited for: CHARACTERIZATION.
[7]	"Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed] [Europe PMC] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[8]	"The nucleolar protein Nop19p interacts preferentially with Utp25p and Dhr2p and is essential for the production of the 40S ribosomal subunit in Saccharomyces cerevisiae." Choque E., Marcellin M., Burlet-Schiltz O., Gadal O., Dez C. RNA Biol. 8:1158-1172(2011) [PubMed] [Europe PMC] [Abstract] Cited for: INTERACTION WITH NOP19.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AF005090 Genomic DNA. Translation: AAB61670.1. L15328 Genomic DNA. Translation: AAA34986.1. X75560 Genomic DNA. Translation: CAA53239.1. Z28078 Genomic DNA. Translation: CAA81915.1. BK006944 Genomic DNA. Translation: DAA09079.1.
PIR	S37903.
RefSeq	NP_012845.1. NM_001179644.1.
3D structure databases
ProteinModelPortal	P36009.
SMR	P36009. Positions 58-719.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-6424N.
IntAct	P36009. 4 interactions.
MINT	MINT-677951.
STRING	4932.YKL078W.
Proteomic databases
PaxDb	P36009.
PeptideAtlas	P36009.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblFungi	YKL078W; YKL078W; YKL078W.
GeneID	853784.
KEGG	sce:YKL078W.
Organism-specific databases
CYGD	YKL078w.
SGD	S000001561. DHR2.
Phylogenomic databases
eggNOG	COG1643.
GeneTree	ENSGT00700000104393.
HOGENOM	HOG000175261.
KO	K14781.
OMA	PGQEEID.
OrthoDB	EOG4F7RTB.
Gene expression databases
Genevestigator	P36009.
GermOnline	YKL078W. Saccharomyces cerevisiae.
Family and domain databases
InterPro	IPR011545. DNA/RNA_helicase_DEAD/DEAH_N. IPR002464. DNA/RNA_helicase_DEAH_CS. IPR011709. DUF1605. IPR007502. Helicase-assoc_dom. IPR014001. Helicase_ATP-bd. IPR001650. Helicase_C. [Graphical view]
Pfam	PF00270. DEAD. 1 hit. PF04408. HA2. 1 hit. PF00271. Helicase_C. 1 hit. PF07717. OB_NTP_bind. 1 hit. [Graphical view]
SMART	SM00487. DEXDc. 1 hit. SM00847. HA2. 1 hit. SM00490. HELICc. 1 hit. [Graphical view]
PROSITE	PS00690. DEAH_ATP_HELICASE. 1 hit. PS51192. HELICASE_ATP_BIND_1. 1 hit. PS51194. HELICASE_CTER. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	974906.

Entry information

Entry name

DHR2_YEAST

Accession

Primary (citable) accession number: P36009
Secondary accession number(s): D6VXK9, Q07040

Entry history

Integrated into UniProtKB/Swiss-Prot:	June 1, 1994
Last sequence update:	June 1, 1994
Last modified:	May 1, 2013
This is version 127 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program