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Protein

Probable ATP-dependent RNA helicase DHR2

Gene

DHR2

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Probable ATP-binding RNA helicase. Required for 18S rRNA synthesis.

Catalytic activityi

ATP + H2O = ADP + phosphate.

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi104 – 1118ATPPROSITE-ProRule annotation

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-KW
  2. ATP-dependent helicase activity Source: InterPro
  3. RNA binding Source: UniProtKB-KW
  4. RNA helicase activity Source: SGD

GO - Biological processi

  1. maturation of SSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) Source: SGD
  2. ribosome biogenesis Source: SGD
Complete GO annotation...

Keywords - Molecular functioni

Helicase, Hydrolase

Keywords - Biological processi

rRNA processing

Keywords - Ligandi

ATP-binding, Nucleotide-binding, RNA-binding

Enzyme and pathway databases

BioCyciYEAST:G3O-31873-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Probable ATP-dependent RNA helicase DHR2 (EC:3.6.4.13)
Alternative name(s):
DEAH box RNA helicase DHR2
Helicase JA2
Gene namesi
Name:DHR2
Ordered Locus Names:YKL078W
ORF Names:YKL408
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
ProteomesiUP000002311: Chromosome XI

Organism-specific databases

CYGDiYKL078w.
SGDiS000001561. DHR2.

Subcellular locationi

GO - Cellular componenti

  1. nucleolus Source: SGD
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 735735Probable ATP-dependent RNA helicase DHR2PRO_0000055163Add
BLAST

Proteomic databases

MaxQBiP36009.
PaxDbiP36009.
PeptideAtlasiP36009.

Expressioni

Gene expression databases

GenevestigatoriP36009.

Interactioni

Subunit structurei

Interacts with NOP19.1 Publication

Protein-protein interaction databases

BioGridi34054. 34 interactions.
DIPiDIP-6424N.
IntActiP36009. 2 interactions.
MINTiMINT-677951.
STRINGi4932.YKL078W.

Structurei

3D structure databases

ProteinModelPortaliP36009.
SMRiP36009. Positions 58-719.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini91 – 257167Helicase ATP-bindingPROSITE-ProRule annotationAdd
BLAST
Domaini262 – 456195Helicase C-terminalPROSITE-ProRule annotationAdd
BLAST

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi203 – 2064DEAH box

Sequence similaritiesi

Contains 1 helicase ATP-binding domain.PROSITE-ProRule annotation
Contains 1 helicase C-terminal domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiCOG1643.
GeneTreeiENSGT00760000120058.
HOGENOMiHOG000175261.
InParanoidiP36009.
KOiK14781.
OMAiEAIMYNE.
OrthoDBiEOG7M98QW.

Family and domain databases

Gene3Di3.40.50.300. 2 hits.
InterProiIPR011545. DEAD/DEAH_box_helicase_dom.
IPR002464. DNA/RNA_helicase_DEAH_CS.
IPR011709. DUF1605.
IPR007502. Helicase-assoc_dom.
IPR014001. Helicase_ATP-bd.
IPR001650. Helicase_C.
IPR027417. P-loop_NTPase.
[Graphical view]
PfamiPF00270. DEAD. 1 hit.
PF04408. HA2. 1 hit.
PF00271. Helicase_C. 1 hit.
PF07717. OB_NTP_bind. 1 hit.
[Graphical view]
SMARTiSM00487. DEXDc. 1 hit.
SM00847. HA2. 1 hit.
SM00490. HELICc. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 1 hit.
PROSITEiPS00690. DEAH_ATP_HELICASE. 1 hit.
PS51192. HELICASE_ATP_BIND_1. 1 hit.
PS51194. HELICASE_CTER. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P36009-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MAANSNSRVA SNHTSKKQKV RRNIHPFTNN TRIKRASKIV KFNDSGEGDH
60 70 80 90 100
VSDQRSNKEN VLTYKSLKSR ASDLLKMRET LPVYQHKREI MSYIESNPVT
110 120 130 140 150
VLIGETGSGK STQIPQFVLE KLYDTKKHGS IAVTQPRRVA AINLATRVAQ
160 170 180 190 200
EHGCKLGEQV GYSVRFDNTT TTRTRLKYLT DGMLLRELMM NSDLREYSVI
210 220 230 240 250
VIDEAHERTV LTDLILGFLK SLIQGPRPDL RIIVMSATLQ AEKFSEFFNN
260 270 280 290 300
APILFVEGRK FDVKQYYLKA PTDDIVDAVI RCCIQINQGE ELGDILCFLP
310 320 330 340 350
GQEEIDKAVT IMEKIAKYVS DEAPVPLIVP YPLYAALPAV QQSLVFAPIK
360 370 380 390 400
GFKRKVVFST NIAETSVTIS GVKFVVDSGL RKVKVWRHQL GLATLLTVPI
410 420 430 440 450
SQASAMQRSG RAGRESEGKS FRLYCESDYV KLPKQSEPEI ARSDVTSPVL
460 470 480 490 500
MLKRYGVDDL LNWTWFENPG KEAIVMGLQE LYELGALDTR GKITKRGQQM
510 520 530 540 550
ALLPLQPHLS SVLIKASEVG CLSQVIDIVS CLSVENLLLN PSPEERDEVN
560 570 580 590 600
ERRLSLCNAG KRYGDLIMLK ELFDIYFYEL GKSQDASSER NDWCKGLCIS
610 620 630 640 650
IRGFKNVIRV RDQLRVYCKR LFSSISEEDE ESKKIGEDGE LISKILKCFL
660 670 680 690 700
TGFIKNTAIG MPDRSYRTVS TGEPISIHPS SMLFMNKSCP GIMYTEYVFT
710 720 730
TKGYARNVSR IELSWLQEVV TNAAAVAKQK VSDSK
Length:735
Mass (Da):82,713
Last modified:June 1, 1994 - v1
Checksum:iAAFA78B5F6215D89
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti28 – 281T → M in AAA34986. 1 PublicationCurated
Sequence conflicti80 – 9819TLPVY…IESNP → HFRLPTQARNNVIYSKQS in AAA34986. 1 PublicationCuratedAdd
BLAST
Sequence conflicti277 – 28812DAVIR…IQINQ → ALSSGVYTNKP in AAA34986. 1 PublicationCuratedAdd
BLAST
Sequence conflicti339 – 3391A → P in AAA34986. 1 PublicationCurated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF005090 Genomic DNA. Translation: AAB61670.1.
L15328 Genomic DNA. Translation: AAA34986.1.
X75560 Genomic DNA. Translation: CAA53239.1.
Z28078 Genomic DNA. Translation: CAA81915.1.
BK006944 Genomic DNA. Translation: DAA09079.1.
PIRiS37903.
RefSeqiNP_012845.1. NM_001179644.1.

Genome annotation databases

EnsemblFungiiYKL078W; YKL078W; YKL078W.
GeneIDi853784.
KEGGisce:YKL078W.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF005090 Genomic DNA. Translation: AAB61670.1.
L15328 Genomic DNA. Translation: AAA34986.1.
X75560 Genomic DNA. Translation: CAA53239.1.
Z28078 Genomic DNA. Translation: CAA81915.1.
BK006944 Genomic DNA. Translation: DAA09079.1.
PIRiS37903.
RefSeqiNP_012845.1. NM_001179644.1.

3D structure databases

ProteinModelPortaliP36009.
SMRiP36009. Positions 58-719.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi34054. 34 interactions.
DIPiDIP-6424N.
IntActiP36009. 2 interactions.
MINTiMINT-677951.
STRINGi4932.YKL078W.

Proteomic databases

MaxQBiP36009.
PaxDbiP36009.
PeptideAtlasiP36009.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYKL078W; YKL078W; YKL078W.
GeneIDi853784.
KEGGisce:YKL078W.

Organism-specific databases

CYGDiYKL078w.
SGDiS000001561. DHR2.

Phylogenomic databases

eggNOGiCOG1643.
GeneTreeiENSGT00760000120058.
HOGENOMiHOG000175261.
InParanoidiP36009.
KOiK14781.
OMAiEAIMYNE.
OrthoDBiEOG7M98QW.

Enzyme and pathway databases

BioCyciYEAST:G3O-31873-MONOMER.

Miscellaneous databases

NextBioi974906.
PROiP36009.

Gene expression databases

GenevestigatoriP36009.

Family and domain databases

Gene3Di3.40.50.300. 2 hits.
InterProiIPR011545. DEAD/DEAH_box_helicase_dom.
IPR002464. DNA/RNA_helicase_DEAH_CS.
IPR011709. DUF1605.
IPR007502. Helicase-assoc_dom.
IPR014001. Helicase_ATP-bd.
IPR001650. Helicase_C.
IPR027417. P-loop_NTPase.
[Graphical view]
PfamiPF00270. DEAD. 1 hit.
PF04408. HA2. 1 hit.
PF00271. Helicase_C. 1 hit.
PF07717. OB_NTP_bind. 1 hit.
[Graphical view]
SMARTiSM00487. DEXDc. 1 hit.
SM00847. HA2. 1 hit.
SM00490. HELICc. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 1 hit.
PROSITEiPS00690. DEAH_ATP_HELICASE. 1 hit.
PS51192. HELICASE_ATP_BIND_1. 1 hit.
PS51194. HELICASE_CTER. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "JA2: a DEAH family RNA-helicase-like gene from Saccharomyces cerevisiae."
    Arenas J.E., Messenger J.
    Submitted (JUL-1997) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  2. "A clone isolated from a Clontech HeLa cDNA library has high homology to yeast DEAH helicases and hybridizes to Northern and Southern blots from yeast but not from HeLa."
    Raju V.S., Datta P.K., Beales M.H., Ghoshal K., Jacob S.T.
    Submitted (NOV-1993) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  3. "Sequence analysis of a 10 kb fragment of yeast chromosome XI identifies the SMY1 locus and reveals sequences related to a pre-mRNA splicing factor and vacuolar ATPase subunit C plus a number of unidentified open reading frames."
    James C.M., Gent M.E., Indge K.J., Oliver S.G.
    Yeast 10:247-255(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  4. "Complete DNA sequence of yeast chromosome XI."
    Dujon B., Alexandraki D., Andre B., Ansorge W., Baladron V., Ballesta J.P.G., Banrevi A., Bolle P.-A., Bolotin-Fukuhara M., Bossier P., Bou G., Boyer J., Buitrago M.J., Cheret G., Colleaux L., Daignan-Fornier B., del Rey F., Dion C.
    , Domdey H., Duesterhoeft A., Duesterhus S., Entian K.-D., Erfle H., Esteban P.F., Feldmann H., Fernandes L., Fobo G.M., Fritz C., Fukuhara H., Gabel C., Gaillon L., Garcia-Cantalejo J.M., Garcia-Ramirez J.J., Gent M.E., Ghazvini M., Goffeau A., Gonzalez A., Grothues D., Guerreiro P., Hegemann J.H., Hewitt N., Hilger F., Hollenberg C.P., Horaitis O., Indge K.J., Jacquier A., James C.M., Jauniaux J.-C., Jimenez A., Keuchel H., Kirchrath L., Kleine K., Koetter P., Legrain P., Liebl S., Louis E.J., Maia e Silva A., Marck C., Monnier A.-L., Moestl D., Mueller S., Obermaier B., Oliver S.G., Pallier C., Pascolo S., Pfeiffer F., Philippsen P., Planta R.J., Pohl F.M., Pohl T.M., Poehlmann R., Portetelle D., Purnelle B., Puzos V., Ramezani Rad M., Rasmussen S.W., Remacha M.A., Revuelta J.L., Richard G.-F., Rieger M., Rodrigues-Pousada C., Rose M., Rupp T., Santos M.A., Schwager C., Sensen C., Skala J., Soares H., Sor F., Stegemann J., Tettelin H., Thierry A., Tzermia M., Urrestarazu L.A., van Dyck L., van Vliet-Reedijk J.C., Valens M., Vandenbol M., Vilela C., Vissers S., von Wettstein D., Voss H., Wiemann S., Xu G., Zimmermann J., Haasemann M., Becker I., Mewes H.-W.
    Nature 369:371-378(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  5. Cited for: GENOME REANNOTATION.
    Strain: ATCC 204508 / S288c.
  6. "Dhr1p, a putative DEAH-Box RNA helicase, is associated with the box C+D snoRNP U3."
    Colley A., Beggs J.D., Tollervey D., Lafontaine D.L.J.
    Mol. Cell. Biol. 20:7238-7246(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: CHARACTERIZATION.
  7. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
  8. "The nucleolar protein Nop19p interacts preferentially with Utp25p and Dhr2p and is essential for the production of the 40S ribosomal subunit in Saccharomyces cerevisiae."
    Choque E., Marcellin M., Burlet-Schiltz O., Gadal O., Dez C.
    RNA Biol. 8:1158-1172(2011) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH NOP19.

Entry informationi

Entry nameiDHR2_YEAST
AccessioniPrimary (citable) accession number: P36009
Secondary accession number(s): D6VXK9, Q07040
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 1, 1994
Last sequence update: June 1, 1994
Last modified: January 7, 2015
This is version 139 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 721 molecules/cell in log phase SD medium.1 Publication

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  3. Yeast chromosome XI
    Yeast (Saccharomyces cerevisiae) chromosome XI: entries and gene names

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.