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Protein

Probable ATP-dependent RNA helicase DHR2

Gene

DHR2

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Probable ATP-binding RNA helicase. Required for 18S rRNA synthesis.

Catalytic activityi

ATP + H2O = ADP + phosphate.

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi104 – 111ATPPROSITE-ProRule annotation8

GO - Molecular functioni

GO - Biological processi

  • maturation of SSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) Source: SGD
  • ribosome biogenesis Source: SGD
  • rRNA methylation Source: Reactome
Complete GO annotation...

Keywords - Molecular functioni

Helicase, Hydrolase

Keywords - Biological processi

rRNA processing

Keywords - Ligandi

ATP-binding, Nucleotide-binding, RNA-binding

Enzyme and pathway databases

BioCyciYEAST:G3O-31873-MONOMER.
ReactomeiR-SCE-6790901. rRNA modification in the nucleus and cytosol.

Names & Taxonomyi

Protein namesi
Recommended name:
Probable ATP-dependent RNA helicase DHR2 (EC:3.6.4.13)
Alternative name(s):
DEAH box RNA helicase DHR2
Helicase JA2
Gene namesi
Name:DHR2
Ordered Locus Names:YKL078W
ORF Names:YKL408
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome XI

Organism-specific databases

EuPathDBiFungiDB:YKL078W.
SGDiS000001561. DHR2.

Subcellular locationi

GO - Cellular componenti

  • cytoplasm Source: GO_Central
  • nucleolus Source: SGD
  • nucleoplasm Source: Reactome
  • spliceosomal complex Source: GO_Central
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000551631 – 735Probable ATP-dependent RNA helicase DHR2Add BLAST735

Proteomic databases

MaxQBiP36009.
PRIDEiP36009.

Interactioni

Subunit structurei

Interacts with NOP19.1 Publication

Protein-protein interaction databases

BioGridi34054. 36 interactors.
DIPiDIP-6424N.
IntActiP36009. 3 interactors.
MINTiMINT-677951.

Structurei

3D structure databases

ProteinModelPortaliP36009.
SMRiP36009.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini91 – 257Helicase ATP-bindingPROSITE-ProRule annotationAdd BLAST167
Domaini262 – 456Helicase C-terminalPROSITE-ProRule annotationAdd BLAST195

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi203 – 206DEAH box4

Sequence similaritiesi

Contains 1 helicase ATP-binding domain.PROSITE-ProRule annotation
Contains 1 helicase C-terminal domain.PROSITE-ProRule annotation

Phylogenomic databases

GeneTreeiENSGT00860000134595.
HOGENOMiHOG000175261.
InParanoidiP36009.
KOiK14781.
OMAiEAIMYNE.
OrthoDBiEOG092C2SAZ.

Family and domain databases

Gene3Di3.40.50.300. 3 hits.
InterProiIPR011545. DEAD/DEAH_box_helicase_dom.
IPR002464. DNA/RNA_helicase_DEAH_CS.
IPR011709. DUF1605.
IPR007502. Helicase-assoc_dom.
IPR014001. Helicase_ATP-bd.
IPR001650. Helicase_C.
IPR027417. P-loop_NTPase.
[Graphical view]
PfamiPF00270. DEAD. 1 hit.
PF04408. HA2. 1 hit.
PF00271. Helicase_C. 1 hit.
PF07717. OB_NTP_bind. 1 hit.
[Graphical view]
SMARTiSM00487. DEXDc. 1 hit.
SM00847. HA2. 1 hit.
SM00490. HELICc. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 1 hit.
PROSITEiPS00690. DEAH_ATP_HELICASE. 1 hit.
PS51192. HELICASE_ATP_BIND_1. 1 hit.
PS51194. HELICASE_CTER. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P36009-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAANSNSRVA SNHTSKKQKV RRNIHPFTNN TRIKRASKIV KFNDSGEGDH
60 70 80 90 100
VSDQRSNKEN VLTYKSLKSR ASDLLKMRET LPVYQHKREI MSYIESNPVT
110 120 130 140 150
VLIGETGSGK STQIPQFVLE KLYDTKKHGS IAVTQPRRVA AINLATRVAQ
160 170 180 190 200
EHGCKLGEQV GYSVRFDNTT TTRTRLKYLT DGMLLRELMM NSDLREYSVI
210 220 230 240 250
VIDEAHERTV LTDLILGFLK SLIQGPRPDL RIIVMSATLQ AEKFSEFFNN
260 270 280 290 300
APILFVEGRK FDVKQYYLKA PTDDIVDAVI RCCIQINQGE ELGDILCFLP
310 320 330 340 350
GQEEIDKAVT IMEKIAKYVS DEAPVPLIVP YPLYAALPAV QQSLVFAPIK
360 370 380 390 400
GFKRKVVFST NIAETSVTIS GVKFVVDSGL RKVKVWRHQL GLATLLTVPI
410 420 430 440 450
SQASAMQRSG RAGRESEGKS FRLYCESDYV KLPKQSEPEI ARSDVTSPVL
460 470 480 490 500
MLKRYGVDDL LNWTWFENPG KEAIVMGLQE LYELGALDTR GKITKRGQQM
510 520 530 540 550
ALLPLQPHLS SVLIKASEVG CLSQVIDIVS CLSVENLLLN PSPEERDEVN
560 570 580 590 600
ERRLSLCNAG KRYGDLIMLK ELFDIYFYEL GKSQDASSER NDWCKGLCIS
610 620 630 640 650
IRGFKNVIRV RDQLRVYCKR LFSSISEEDE ESKKIGEDGE LISKILKCFL
660 670 680 690 700
TGFIKNTAIG MPDRSYRTVS TGEPISIHPS SMLFMNKSCP GIMYTEYVFT
710 720 730
TKGYARNVSR IELSWLQEVV TNAAAVAKQK VSDSK
Length:735
Mass (Da):82,713
Last modified:June 1, 1994 - v1
Checksum:iAAFA78B5F6215D89
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti28T → M in AAA34986 (Ref. 2) Curated1
Sequence conflicti80 – 98TLPVY…IESNP → HFRLPTQARNNVIYSKQS in AAA34986 (Ref. 2) CuratedAdd BLAST19
Sequence conflicti277 – 288DAVIR…IQINQ → ALSSGVYTNKP in AAA34986 (Ref. 2) CuratedAdd BLAST12
Sequence conflicti339A → P in AAA34986 (Ref. 2) Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF005090 Genomic DNA. Translation: AAB61670.1.
L15328 Genomic DNA. Translation: AAA34986.1.
X75560 Genomic DNA. Translation: CAA53239.1.
Z28078 Genomic DNA. Translation: CAA81915.1.
BK006944 Genomic DNA. Translation: DAA09079.1.
PIRiS37903.
RefSeqiNP_012845.1. NM_001179644.1.

Genome annotation databases

EnsemblFungiiYKL078W; YKL078W; YKL078W.
GeneIDi853784.
KEGGisce:YKL078W.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF005090 Genomic DNA. Translation: AAB61670.1.
L15328 Genomic DNA. Translation: AAA34986.1.
X75560 Genomic DNA. Translation: CAA53239.1.
Z28078 Genomic DNA. Translation: CAA81915.1.
BK006944 Genomic DNA. Translation: DAA09079.1.
PIRiS37903.
RefSeqiNP_012845.1. NM_001179644.1.

3D structure databases

ProteinModelPortaliP36009.
SMRiP36009.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi34054. 36 interactors.
DIPiDIP-6424N.
IntActiP36009. 3 interactors.
MINTiMINT-677951.

Proteomic databases

MaxQBiP36009.
PRIDEiP36009.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYKL078W; YKL078W; YKL078W.
GeneIDi853784.
KEGGisce:YKL078W.

Organism-specific databases

EuPathDBiFungiDB:YKL078W.
SGDiS000001561. DHR2.

Phylogenomic databases

GeneTreeiENSGT00860000134595.
HOGENOMiHOG000175261.
InParanoidiP36009.
KOiK14781.
OMAiEAIMYNE.
OrthoDBiEOG092C2SAZ.

Enzyme and pathway databases

BioCyciYEAST:G3O-31873-MONOMER.
ReactomeiR-SCE-6790901. rRNA modification in the nucleus and cytosol.

Miscellaneous databases

PROiP36009.

Family and domain databases

Gene3Di3.40.50.300. 3 hits.
InterProiIPR011545. DEAD/DEAH_box_helicase_dom.
IPR002464. DNA/RNA_helicase_DEAH_CS.
IPR011709. DUF1605.
IPR007502. Helicase-assoc_dom.
IPR014001. Helicase_ATP-bd.
IPR001650. Helicase_C.
IPR027417. P-loop_NTPase.
[Graphical view]
PfamiPF00270. DEAD. 1 hit.
PF04408. HA2. 1 hit.
PF00271. Helicase_C. 1 hit.
PF07717. OB_NTP_bind. 1 hit.
[Graphical view]
SMARTiSM00487. DEXDc. 1 hit.
SM00847. HA2. 1 hit.
SM00490. HELICc. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 1 hit.
PROSITEiPS00690. DEAH_ATP_HELICASE. 1 hit.
PS51192. HELICASE_ATP_BIND_1. 1 hit.
PS51194. HELICASE_CTER. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiDHR2_YEAST
AccessioniPrimary (citable) accession number: P36009
Secondary accession number(s): D6VXK9, Q07040
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 1, 1994
Last sequence update: June 1, 1994
Last modified: November 30, 2016
This is version 154 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 721 molecules/cell in log phase SD medium.1 Publication

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  3. Yeast chromosome XI
    Yeast (Saccharomyces cerevisiae) chromosome XI: entries and gene names

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.