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P36009

- DHR2_YEAST

UniProt

P36009 - DHR2_YEAST

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Protein
Probable ATP-dependent RNA helicase DHR2
Gene
DHR2, YKL078W, YKL408
Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at protein leveli

Functioni

Probable ATP-binding RNA helicase. Required for 18S rRNA synthesis.

Catalytic activityi

ATP + H2O = ADP + phosphate.

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi104 – 1118ATP By similarity

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-KW
  2. ATP-dependent helicase activity Source: InterPro
  3. RNA binding Source: UniProtKB-KW
  4. RNA helicase activity Source: SGD

GO - Biological processi

  1. maturation of SSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) Source: SGD
  2. ribosome biogenesis Source: SGD
Complete GO annotation...

Keywords - Molecular functioni

Helicase, Hydrolase

Keywords - Biological processi

rRNA processing

Keywords - Ligandi

ATP-binding, Nucleotide-binding, RNA-binding

Enzyme and pathway databases

BioCyciYEAST:G3O-31873-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Probable ATP-dependent RNA helicase DHR2 (EC:3.6.4.13)
Alternative name(s):
DEAH box RNA helicase DHR2
Helicase JA2
Gene namesi
Name:DHR2
Ordered Locus Names:YKL078W
ORF Names:YKL408
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
ProteomesiUP000002311: Chromosome XI

Organism-specific databases

CYGDiYKL078w.
SGDiS000001561. DHR2.

Subcellular locationi

GO - Cellular componenti

  1. nucleolus Source: SGD
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 735735Probable ATP-dependent RNA helicase DHR2
PRO_0000055163Add
BLAST

Proteomic databases

MaxQBiP36009.
PaxDbiP36009.
PeptideAtlasiP36009.

Expressioni

Gene expression databases

GenevestigatoriP36009.

Interactioni

Subunit structurei

Interacts with NOP19.1 Publication

Protein-protein interaction databases

BioGridi34054. 33 interactions.
DIPiDIP-6424N.
IntActiP36009. 2 interactions.
MINTiMINT-677951.
STRINGi4932.YKL078W.

Structurei

3D structure databases

ProteinModelPortaliP36009.
SMRiP36009. Positions 58-719.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini91 – 257167Helicase ATP-binding
Add
BLAST
Domaini262 – 456195Helicase C-terminal
Add
BLAST

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi203 – 2064DEAH box

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG1643.
GeneTreeiENSGT00750000118132.
HOGENOMiHOG000175261.
KOiK14781.
OMAiIKFLTEG.
OrthoDBiEOG7M98QW.

Family and domain databases

Gene3Di3.40.50.300. 2 hits.
InterProiIPR011545. DNA/RNA_helicase_DEAD/DEAH_N.
IPR002464. DNA/RNA_helicase_DEAH_CS.
IPR011709. DUF1605.
IPR007502. Helicase-assoc_dom.
IPR014001. Helicase_ATP-bd.
IPR001650. Helicase_C.
IPR027417. P-loop_NTPase.
[Graphical view]
PfamiPF00270. DEAD. 1 hit.
PF04408. HA2. 1 hit.
PF00271. Helicase_C. 1 hit.
PF07717. OB_NTP_bind. 1 hit.
[Graphical view]
SMARTiSM00487. DEXDc. 1 hit.
SM00847. HA2. 1 hit.
SM00490. HELICc. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 1 hit.
PROSITEiPS00690. DEAH_ATP_HELICASE. 1 hit.
PS51192. HELICASE_ATP_BIND_1. 1 hit.
PS51194. HELICASE_CTER. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P36009-1 [UniParc]FASTAAdd to Basket

« Hide

MAANSNSRVA SNHTSKKQKV RRNIHPFTNN TRIKRASKIV KFNDSGEGDH    50
VSDQRSNKEN VLTYKSLKSR ASDLLKMRET LPVYQHKREI MSYIESNPVT 100
VLIGETGSGK STQIPQFVLE KLYDTKKHGS IAVTQPRRVA AINLATRVAQ 150
EHGCKLGEQV GYSVRFDNTT TTRTRLKYLT DGMLLRELMM NSDLREYSVI 200
VIDEAHERTV LTDLILGFLK SLIQGPRPDL RIIVMSATLQ AEKFSEFFNN 250
APILFVEGRK FDVKQYYLKA PTDDIVDAVI RCCIQINQGE ELGDILCFLP 300
GQEEIDKAVT IMEKIAKYVS DEAPVPLIVP YPLYAALPAV QQSLVFAPIK 350
GFKRKVVFST NIAETSVTIS GVKFVVDSGL RKVKVWRHQL GLATLLTVPI 400
SQASAMQRSG RAGRESEGKS FRLYCESDYV KLPKQSEPEI ARSDVTSPVL 450
MLKRYGVDDL LNWTWFENPG KEAIVMGLQE LYELGALDTR GKITKRGQQM 500
ALLPLQPHLS SVLIKASEVG CLSQVIDIVS CLSVENLLLN PSPEERDEVN 550
ERRLSLCNAG KRYGDLIMLK ELFDIYFYEL GKSQDASSER NDWCKGLCIS 600
IRGFKNVIRV RDQLRVYCKR LFSSISEEDE ESKKIGEDGE LISKILKCFL 650
TGFIKNTAIG MPDRSYRTVS TGEPISIHPS SMLFMNKSCP GIMYTEYVFT 700
TKGYARNVSR IELSWLQEVV TNAAAVAKQK VSDSK 735
Length:735
Mass (Da):82,713
Last modified:June 1, 1994 - v1
Checksum:iAAFA78B5F6215D89
GO

Sequence conflict

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti28 – 281T → M in AAA34986. 1 Publication
Sequence conflicti80 – 9819TLPVY…IESNP → HFRLPTQARNNVIYSKQS in AAA34986. 1 Publication
Add
BLAST
Sequence conflicti277 – 28812DAVIR…IQINQ → ALSSGVYTNKP in AAA34986. 1 Publication
Add
BLAST
Sequence conflicti339 – 3391A → P in AAA34986. 1 Publication

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AF005090 Genomic DNA. Translation: AAB61670.1.
L15328 Genomic DNA. Translation: AAA34986.1.
X75560 Genomic DNA. Translation: CAA53239.1.
Z28078 Genomic DNA. Translation: CAA81915.1.
BK006944 Genomic DNA. Translation: DAA09079.1.
PIRiS37903.
RefSeqiNP_012845.1. NM_001179644.1.

Genome annotation databases

EnsemblFungiiYKL078W; YKL078W; YKL078W.
GeneIDi853784.
KEGGisce:YKL078W.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AF005090 Genomic DNA. Translation: AAB61670.1 .
L15328 Genomic DNA. Translation: AAA34986.1 .
X75560 Genomic DNA. Translation: CAA53239.1 .
Z28078 Genomic DNA. Translation: CAA81915.1 .
BK006944 Genomic DNA. Translation: DAA09079.1 .
PIRi S37903.
RefSeqi NP_012845.1. NM_001179644.1.

3D structure databases

ProteinModelPortali P36009.
SMRi P36009. Positions 58-719.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 34054. 33 interactions.
DIPi DIP-6424N.
IntActi P36009. 2 interactions.
MINTi MINT-677951.
STRINGi 4932.YKL078W.

Proteomic databases

MaxQBi P36009.
PaxDbi P36009.
PeptideAtlasi P36009.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblFungii YKL078W ; YKL078W ; YKL078W .
GeneIDi 853784.
KEGGi sce:YKL078W.

Organism-specific databases

CYGDi YKL078w.
SGDi S000001561. DHR2.

Phylogenomic databases

eggNOGi COG1643.
GeneTreei ENSGT00750000118132.
HOGENOMi HOG000175261.
KOi K14781.
OMAi IKFLTEG.
OrthoDBi EOG7M98QW.

Enzyme and pathway databases

BioCyci YEAST:G3O-31873-MONOMER.

Miscellaneous databases

NextBioi 974906.
PROi P36009.

Gene expression databases

Genevestigatori P36009.

Family and domain databases

Gene3Di 3.40.50.300. 2 hits.
InterProi IPR011545. DNA/RNA_helicase_DEAD/DEAH_N.
IPR002464. DNA/RNA_helicase_DEAH_CS.
IPR011709. DUF1605.
IPR007502. Helicase-assoc_dom.
IPR014001. Helicase_ATP-bd.
IPR001650. Helicase_C.
IPR027417. P-loop_NTPase.
[Graphical view ]
Pfami PF00270. DEAD. 1 hit.
PF04408. HA2. 1 hit.
PF00271. Helicase_C. 1 hit.
PF07717. OB_NTP_bind. 1 hit.
[Graphical view ]
SMARTi SM00487. DEXDc. 1 hit.
SM00847. HA2. 1 hit.
SM00490. HELICc. 1 hit.
[Graphical view ]
SUPFAMi SSF52540. SSF52540. 1 hit.
PROSITEi PS00690. DEAH_ATP_HELICASE. 1 hit.
PS51192. HELICASE_ATP_BIND_1. 1 hit.
PS51194. HELICASE_CTER. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "JA2: a DEAH family RNA-helicase-like gene from Saccharomyces cerevisiae."
    Arenas J.E., Messenger J.
    Submitted (JUL-1997) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  2. "A clone isolated from a Clontech HeLa cDNA library has high homology to yeast DEAH helicases and hybridizes to Northern and Southern blots from yeast but not from HeLa."
    Raju V.S., Datta P.K., Beales M.H., Ghoshal K., Jacob S.T.
    Submitted (NOV-1993) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  3. "Sequence analysis of a 10 kb fragment of yeast chromosome XI identifies the SMY1 locus and reveals sequences related to a pre-mRNA splicing factor and vacuolar ATPase subunit C plus a number of unidentified open reading frames."
    James C.M., Gent M.E., Indge K.J., Oliver S.G.
    Yeast 10:247-255(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  4. "Complete DNA sequence of yeast chromosome XI."
    Dujon B., Alexandraki D., Andre B., Ansorge W., Baladron V., Ballesta J.P.G., Banrevi A., Bolle P.-A., Bolotin-Fukuhara M., Bossier P., Bou G., Boyer J., Buitrago M.J., Cheret G., Colleaux L., Daignan-Fornier B., del Rey F., Dion C.
    , Domdey H., Duesterhoeft A., Duesterhus S., Entian K.-D., Erfle H., Esteban P.F., Feldmann H., Fernandes L., Fobo G.M., Fritz C., Fukuhara H., Gabel C., Gaillon L., Garcia-Cantalejo J.M., Garcia-Ramirez J.J., Gent M.E., Ghazvini M., Goffeau A., Gonzalez A., Grothues D., Guerreiro P., Hegemann J.H., Hewitt N., Hilger F., Hollenberg C.P., Horaitis O., Indge K.J., Jacquier A., James C.M., Jauniaux J.-C., Jimenez A., Keuchel H., Kirchrath L., Kleine K., Koetter P., Legrain P., Liebl S., Louis E.J., Maia e Silva A., Marck C., Monnier A.-L., Moestl D., Mueller S., Obermaier B., Oliver S.G., Pallier C., Pascolo S., Pfeiffer F., Philippsen P., Planta R.J., Pohl F.M., Pohl T.M., Poehlmann R., Portetelle D., Purnelle B., Puzos V., Ramezani Rad M., Rasmussen S.W., Remacha M.A., Revuelta J.L., Richard G.-F., Rieger M., Rodrigues-Pousada C., Rose M., Rupp T., Santos M.A., Schwager C., Sensen C., Skala J., Soares H., Sor F., Stegemann J., Tettelin H., Thierry A., Tzermia M., Urrestarazu L.A., van Dyck L., van Vliet-Reedijk J.C., Valens M., Vandenbol M., Vilela C., Vissers S., von Wettstein D., Voss H., Wiemann S., Xu G., Zimmermann J., Haasemann M., Becker I., Mewes H.-W.
    Nature 369:371-378(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  5. Cited for: GENOME REANNOTATION.
    Strain: ATCC 204508 / S288c.
  6. "Dhr1p, a putative DEAH-Box RNA helicase, is associated with the box C+D snoRNP U3."
    Colley A., Beggs J.D., Tollervey D., Lafontaine D.L.J.
    Mol. Cell. Biol. 20:7238-7246(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: CHARACTERIZATION.
  7. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
  8. "The nucleolar protein Nop19p interacts preferentially with Utp25p and Dhr2p and is essential for the production of the 40S ribosomal subunit in Saccharomyces cerevisiae."
    Choque E., Marcellin M., Burlet-Schiltz O., Gadal O., Dez C.
    RNA Biol. 8:1158-1172(2011) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH NOP19.

Entry informationi

Entry nameiDHR2_YEAST
AccessioniPrimary (citable) accession number: P36009
Secondary accession number(s): D6VXK9, Q07040
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 1, 1994
Last sequence update: June 1, 1994
Last modified: May 14, 2014
This is version 136 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 721 molecules/cell in log phase SD medium.

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  3. Yeast chromosome XI
    Yeast (Saccharomyces cerevisiae) chromosome XI: entries and gene names

External Data

Dasty 3

Similar proteinsi