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Reviewed, UniProtKB/Swiss-Prot P36009 (DHR2_YEAST)

Last modified November 24, 2009. Version 93. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data | Customize display text xml rdf/xml gff fasta
Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Binary interactions · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents

Names and origin

Protein namesRecommended name:
    Probable ATP-dependent RNA helicase DHR2
    EC=3.6.1.-
Alternative name(s):
    DEAH box RNA helicase DHR2
    Helicase JA2
Gene names
Name: DHR2
Ordered Locus Names: YKL078W
ORF Names: YKL408
OrganismSaccharomyces cerevisiae (Baker's yeast) [Complete proteome]
Taxonomic identifier4932 [NCBI]
Taxonomic lineageEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces

Protein attributes

Sequence length735 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is not processed.
Protein existenceEvidence at protein level.

General annotation (Comments)

Function

Probable ATP-binding RNA helicase. Required for 18S rRNA synthesis.

Subcellular location

Nucleusnucleolus.

Miscellaneous

Present with 721 molecules/cell in log phase SD medium. Ref.6

Sequence similarities

Belongs to the DEAD box helicase family. DEAH subfamily.

Contains 1 helicase ATP-binding domain.

Contains 1 helicase C-terminal domain.

Binary interactions

With

Entry

#Exp.

IntAct

Notes

SPT8P389151EBI-5844,EBI-17964
UBP10P538741EBI-5844,EBI-19873

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 735735Probable ATP-dependent RNA helicase DHR2
PRO_0000055163

Regions

Domain91 – 257167Helicase ATP-binding
Domain262 – 456195Helicase C-terminal
Nucleotide binding104 – 1118ATP By similarity
Motif203 – 2064DEAH box

Experimental info

Sequence conflict281T → M in AAA34986. Ref.2
Sequence conflict80 – 9819TLPVY…IESNP → HFRLPTQARNNVIYSKQS in AAA34986. Ref.2
Sequence conflict277 – 28812DAVIR…IQINQ → ALSSGVYTNKP in AAA34986. Ref.2
Sequence conflict3391A → P in AAA34986. Ref.2

Sequences

Sequence LengthMass (Da)Tools
P36009-1 [UniParc].

Last modified June 1, 1994. Version 1.
Checksum: AAFA78B5F6215D89

FASTA73582,713
        10         20         30         40         50         60 
MAANSNSRVA SNHTSKKQKV RRNIHPFTNN TRIKRASKIV KFNDSGEGDH VSDQRSNKEN 

        70         80         90        100        110        120 
VLTYKSLKSR ASDLLKMRET LPVYQHKREI MSYIESNPVT VLIGETGSGK STQIPQFVLE 

       130        140        150        160        170        180 
KLYDTKKHGS IAVTQPRRVA AINLATRVAQ EHGCKLGEQV GYSVRFDNTT TTRTRLKYLT 

       190        200        210        220        230        240 
DGMLLRELMM NSDLREYSVI VIDEAHERTV LTDLILGFLK SLIQGPRPDL RIIVMSATLQ 

       250        260        270        280        290        300 
AEKFSEFFNN APILFVEGRK FDVKQYYLKA PTDDIVDAVI RCCIQINQGE ELGDILCFLP 

       310        320        330        340        350        360 
GQEEIDKAVT IMEKIAKYVS DEAPVPLIVP YPLYAALPAV QQSLVFAPIK GFKRKVVFST 

       370        380        390        400        410        420 
NIAETSVTIS GVKFVVDSGL RKVKVWRHQL GLATLLTVPI SQASAMQRSG RAGRESEGKS 

       430        440        450        460        470        480 
FRLYCESDYV KLPKQSEPEI ARSDVTSPVL MLKRYGVDDL LNWTWFENPG KEAIVMGLQE 

       490        500        510        520        530        540 
LYELGALDTR GKITKRGQQM ALLPLQPHLS SVLIKASEVG CLSQVIDIVS CLSVENLLLN 

       550        560        570        580        590        600 
PSPEERDEVN ERRLSLCNAG KRYGDLIMLK ELFDIYFYEL GKSQDASSER NDWCKGLCIS 

       610        620        630        640        650        660 
IRGFKNVIRV RDQLRVYCKR LFSSISEEDE ESKKIGEDGE LISKILKCFL TGFIKNTAIG 

       670        680        690        700        710        720 
MPDRSYRTVS TGEPISIHPS SMLFMNKSCP GIMYTEYVFT TKGYARNVSR IELSWLQEVV 

       730 
TNAAAVAKQK VSDSK 

« Hide

References

« Hide 'large scale' references
[1]"JA2: a DEAH family RNA-helicase-like gene from Saccharomyces cerevisiae."
Arenas J.E., Messenger J.
Submitted (JUL-1997) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]"A clone isolated from a Clontech HeLa cDNA library has high homology to yeast DEAH helicases and hybridizes to Northern and Southern blots from yeast but not from HeLa."
Raju V.S., Datta P.K., Beales M.H., Ghoshal K., Jacob S.T.
Submitted (NOV-1993) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[3]"Sequence analysis of a 10 kb fragment of yeast chromosome XI identifies the SMY1 locus and reveals sequences related to a pre-mRNA splicing factor and vacuolar ATPase subunit C plus a number of unidentified open reading frames."
James C.M., Gent M.E., Indge K.J., Oliver S.G.
Yeast 10:247-255(1994) [PubMed: 8203165] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[4]"Complete DNA sequence of yeast chromosome XI."
Dujon B., Alexandraki D., Andre B., Ansorge W., Baladron V., Ballesta J.P.G., Banrevi A., Bolle P.-A., Bolotin-Fukuhara M., Bossier P., Bou G., Boyer J., Buitrago M.J., Cheret G., Colleaux L., Daignan-Fornier B., del Rey F., Dion C. expand/collapse author list , Domdey H., Duesterhoeft A., Duesterhus S., Entian K.-D., Erfle H., Esteban P.F., Feldmann H., Fernandes L., Fobo G.M., Fritz C., Fukuhara H., Gabel C., Gaillon L., Garcia-Cantalejo J.M., Garcia-Ramirez J.J., Gent M.E., Ghazvini M., Goffeau A., Gonzalez A., Grothues D., Guerreiro P., Hegemann J.H., Hewitt N., Hilger F., Hollenberg C.P., Horaitis O., Indge K.J., Jacquier A., James C.M., Jauniaux J.-C., Jimenez A., Keuchel H., Kirchrath L., Kleine K., Koetter P., Legrain P., Liebl S., Louis E.J., Maia e Silva A., Marck C., Monnier A.-L., Moestl D., Mueller S., Obermaier B., Oliver S.G., Pallier C., Pascolo S., Pfeiffer F., Philippsen P., Planta R.J., Pohl F.M., Pohl T.M., Poehlmann R., Portetelle D., Purnelle B., Puzos V., Ramezani Rad M., Rasmussen S.W., Remacha M.A., Revuelta J.L., Richard G.-F., Rieger M., Rodrigues-Pousada C., Rose M., Rupp T., Santos M.A., Schwager C., Sensen C., Skala J., Soares H., Sor F., Stegemann J., Tettelin H., Thierry A., Tzermia M., Urrestarazu L.A., van Dyck L., van Vliet-Reedijk J.C., Valens M., Vandenbol M., Vilela C., Vissers S., von Wettstein D., Voss H., Wiemann S., Xu G., Zimmermann J., Haasemann M., Becker I., Mewes H.-W.
Nature 369:371-378(1994) [PubMed: 8196765] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 96604 / S288c / FY1679.
[5]"Dhr1p, a putative DEAH-Box RNA helicase, is associated with the box C+D snoRNP U3."
Colley A., Beggs J.D., Tollervey D., Lafontaine D.L.J.
Mol. Cell. Biol. 20:7238-7246(2000) [PubMed: 10982841] [Abstract]
Cited for: CHARACTERIZATION.
[6]"Global analysis of protein expression in yeast."
Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S.
Nature 425:737-741(2003) [PubMed: 14562106] [Abstract]
Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
+Additional computationally mapped references.

Cross-references

Sequence databases

AF005090 Genomic DNA. Translation: AAB61670.1.
L15328 Genomic DNA. Translation: AAA34986.1.
X75560 Genomic DNA. Translation: CAA53239.1.
Z28078 Genomic DNA. Translation: CAA81915.1.
PIRS37903.
RefSeqNP_012845.1.

3D structure databases

ModBaseSearch...

Protein-protein interaction databases

DIPDIP:6424N.
IntActP36009. 4 interactions.
STRINGP36009.

Proteomic databases

PeptideAtlasP36009.
PRIDEP36009.

Genome annotation databases

EnsemblYKL078W; YKL078W; YKL078W; Saccharomyces cerevisiae. [Genome view]
GeneID853784.
KEGGsce:YKL078W.
NMPDRfig|4932.3.peg.3829.

Organism-specific databases

CYGDYKL078w.
SGDS000001561. DHR2.

Phylogenomic databases

HOGENOMP36009.
OMAPDRSYRT
OrthoDBEOG9KPVT1

Gene expression databases

ArrayExpressP36009.
GenevestigatorP36009.
GermOnlineYKL078W. Saccharomyces cerevisiae.

Family and domain databases

InterProIPR014001. DEAD-like_N.
IPR001650. DNA/RNA_helicase_C.
IPR002464. DNA/RNA_helicase_DEAH_CS.
IPR011709. DUF1605.
IPR007502. Helicase-assoc_reg.
IPR014021. Helicase_SF1/SF2_ATP-bd.
[Graphical view]
PfamPF07717. DUF1605. 1 hit.
PF04408. HA2. 1 hit.
PF00271. Helicase_C. 1 hit.
[Graphical view]
SMARTSM00487. DEXDc. 1 hit.
SM00847. HA2. 1 hit.
SM00490. HELICc. 1 hit.
[Graphical view]
PROSITEPS00690. DEAH_ATP_HELICASE. 1 hit.
PS51192. HELICASE_ATP_BIND_1. 1 hit.
PS51194. HELICASE_CTER. 1 hit.
[Graphical view]
ProtoNetSearch...

Other Resources

NextBio974906.

Entry information

Entry nameDHR2_YEAST
AccessionPrimary (citable) accession number: P36009
Secondary accession number(s): Q07040
Entry history
Integrated into UniProtKB/Swiss-Prot: June 1, 1994
Last sequence update: June 1, 1994
Last modified: November 24, 2009
This is version 93 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation projectFPAP (Fungal Proteome Annotation Project)

Relevant documents

SIMILARITY comments

Index of protein domains and families

Yeast

Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD

Yeast chromosome XI

Yeast (Saccharomyces cerevisiae) chromosome XI: entries and gene names

Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Binary interactions · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents