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P36009

- DHR2_YEAST

UniProt

P36009 - DHR2_YEAST

Protein

Probable ATP-dependent RNA helicase DHR2

Gene

DHR2

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 137 (01 Oct 2014)
      Sequence version 1 (01 Jun 1994)
      Previous versions | rss
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    Functioni

    Probable ATP-binding RNA helicase. Required for 18S rRNA synthesis.

    Catalytic activityi

    ATP + H2O = ADP + phosphate.

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi104 – 1118ATPPROSITE-ProRule annotation

    GO - Molecular functioni

    1. ATP binding Source: UniProtKB-KW
    2. ATP-dependent helicase activity Source: InterPro
    3. RNA binding Source: UniProtKB-KW
    4. RNA helicase activity Source: SGD

    GO - Biological processi

    1. maturation of SSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) Source: SGD
    2. ribosome biogenesis Source: SGD

    Keywords - Molecular functioni

    Helicase, Hydrolase

    Keywords - Biological processi

    rRNA processing

    Keywords - Ligandi

    ATP-binding, Nucleotide-binding, RNA-binding

    Enzyme and pathway databases

    BioCyciYEAST:G3O-31873-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Probable ATP-dependent RNA helicase DHR2 (EC:3.6.4.13)
    Alternative name(s):
    DEAH box RNA helicase DHR2
    Helicase JA2
    Gene namesi
    Name:DHR2
    Ordered Locus Names:YKL078W
    ORF Names:YKL408
    OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
    Taxonomic identifieri559292 [NCBI]
    Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
    ProteomesiUP000002311: Chromosome XI

    Organism-specific databases

    CYGDiYKL078w.
    SGDiS000001561. DHR2.

    Subcellular locationi

    GO - Cellular componenti

    1. nucleolus Source: SGD

    Keywords - Cellular componenti

    Nucleus

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 735735Probable ATP-dependent RNA helicase DHR2PRO_0000055163Add
    BLAST

    Proteomic databases

    MaxQBiP36009.
    PaxDbiP36009.
    PeptideAtlasiP36009.

    Expressioni

    Gene expression databases

    GenevestigatoriP36009.

    Interactioni

    Subunit structurei

    Interacts with NOP19.1 Publication

    Protein-protein interaction databases

    BioGridi34054. 33 interactions.
    DIPiDIP-6424N.
    IntActiP36009. 2 interactions.
    MINTiMINT-677951.
    STRINGi4932.YKL078W.

    Structurei

    3D structure databases

    ProteinModelPortaliP36009.
    SMRiP36009. Positions 58-719.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini91 – 257167Helicase ATP-bindingPROSITE-ProRule annotationAdd
    BLAST
    Domaini262 – 456195Helicase C-terminalPROSITE-ProRule annotationAdd
    BLAST

    Motif

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Motifi203 – 2064DEAH box

    Sequence similaritiesi

    Contains 1 helicase ATP-binding domain.PROSITE-ProRule annotation
    Contains 1 helicase C-terminal domain.PROSITE-ProRule annotation

    Phylogenomic databases

    eggNOGiCOG1643.
    GeneTreeiENSGT00750000118132.
    HOGENOMiHOG000175261.
    KOiK14781.
    OMAiIKFLTEG.
    OrthoDBiEOG7M98QW.

    Family and domain databases

    Gene3Di3.40.50.300. 2 hits.
    InterProiIPR011545. DNA/RNA_helicase_DEAD/DEAH_N.
    IPR002464. DNA/RNA_helicase_DEAH_CS.
    IPR011709. DUF1605.
    IPR007502. Helicase-assoc_dom.
    IPR014001. Helicase_ATP-bd.
    IPR001650. Helicase_C.
    IPR027417. P-loop_NTPase.
    [Graphical view]
    PfamiPF00270. DEAD. 1 hit.
    PF04408. HA2. 1 hit.
    PF00271. Helicase_C. 1 hit.
    PF07717. OB_NTP_bind. 1 hit.
    [Graphical view]
    SMARTiSM00487. DEXDc. 1 hit.
    SM00847. HA2. 1 hit.
    SM00490. HELICc. 1 hit.
    [Graphical view]
    SUPFAMiSSF52540. SSF52540. 1 hit.
    PROSITEiPS00690. DEAH_ATP_HELICASE. 1 hit.
    PS51192. HELICASE_ATP_BIND_1. 1 hit.
    PS51194. HELICASE_CTER. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P36009-1 [UniParc]FASTAAdd to Basket

    « Hide

    MAANSNSRVA SNHTSKKQKV RRNIHPFTNN TRIKRASKIV KFNDSGEGDH    50
    VSDQRSNKEN VLTYKSLKSR ASDLLKMRET LPVYQHKREI MSYIESNPVT 100
    VLIGETGSGK STQIPQFVLE KLYDTKKHGS IAVTQPRRVA AINLATRVAQ 150
    EHGCKLGEQV GYSVRFDNTT TTRTRLKYLT DGMLLRELMM NSDLREYSVI 200
    VIDEAHERTV LTDLILGFLK SLIQGPRPDL RIIVMSATLQ AEKFSEFFNN 250
    APILFVEGRK FDVKQYYLKA PTDDIVDAVI RCCIQINQGE ELGDILCFLP 300
    GQEEIDKAVT IMEKIAKYVS DEAPVPLIVP YPLYAALPAV QQSLVFAPIK 350
    GFKRKVVFST NIAETSVTIS GVKFVVDSGL RKVKVWRHQL GLATLLTVPI 400
    SQASAMQRSG RAGRESEGKS FRLYCESDYV KLPKQSEPEI ARSDVTSPVL 450
    MLKRYGVDDL LNWTWFENPG KEAIVMGLQE LYELGALDTR GKITKRGQQM 500
    ALLPLQPHLS SVLIKASEVG CLSQVIDIVS CLSVENLLLN PSPEERDEVN 550
    ERRLSLCNAG KRYGDLIMLK ELFDIYFYEL GKSQDASSER NDWCKGLCIS 600
    IRGFKNVIRV RDQLRVYCKR LFSSISEEDE ESKKIGEDGE LISKILKCFL 650
    TGFIKNTAIG MPDRSYRTVS TGEPISIHPS SMLFMNKSCP GIMYTEYVFT 700
    TKGYARNVSR IELSWLQEVV TNAAAVAKQK VSDSK 735
    Length:735
    Mass (Da):82,713
    Last modified:June 1, 1994 - v1
    Checksum:iAAFA78B5F6215D89
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti28 – 281T → M in AAA34986. 1 PublicationCurated
    Sequence conflicti80 – 9819TLPVY…IESNP → HFRLPTQARNNVIYSKQS in AAA34986. 1 PublicationCuratedAdd
    BLAST
    Sequence conflicti277 – 28812DAVIR…IQINQ → ALSSGVYTNKP in AAA34986. 1 PublicationCuratedAdd
    BLAST
    Sequence conflicti339 – 3391A → P in AAA34986. 1 PublicationCurated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF005090 Genomic DNA. Translation: AAB61670.1.
    L15328 Genomic DNA. Translation: AAA34986.1.
    X75560 Genomic DNA. Translation: CAA53239.1.
    Z28078 Genomic DNA. Translation: CAA81915.1.
    BK006944 Genomic DNA. Translation: DAA09079.1.
    PIRiS37903.
    RefSeqiNP_012845.1. NM_001179644.1.

    Genome annotation databases

    EnsemblFungiiYKL078W; YKL078W; YKL078W.
    GeneIDi853784.
    KEGGisce:YKL078W.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF005090 Genomic DNA. Translation: AAB61670.1 .
    L15328 Genomic DNA. Translation: AAA34986.1 .
    X75560 Genomic DNA. Translation: CAA53239.1 .
    Z28078 Genomic DNA. Translation: CAA81915.1 .
    BK006944 Genomic DNA. Translation: DAA09079.1 .
    PIRi S37903.
    RefSeqi NP_012845.1. NM_001179644.1.

    3D structure databases

    ProteinModelPortali P36009.
    SMRi P36009. Positions 58-719.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 34054. 33 interactions.
    DIPi DIP-6424N.
    IntActi P36009. 2 interactions.
    MINTi MINT-677951.
    STRINGi 4932.YKL078W.

    Proteomic databases

    MaxQBi P36009.
    PaxDbi P36009.
    PeptideAtlasi P36009.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblFungii YKL078W ; YKL078W ; YKL078W .
    GeneIDi 853784.
    KEGGi sce:YKL078W.

    Organism-specific databases

    CYGDi YKL078w.
    SGDi S000001561. DHR2.

    Phylogenomic databases

    eggNOGi COG1643.
    GeneTreei ENSGT00750000118132.
    HOGENOMi HOG000175261.
    KOi K14781.
    OMAi IKFLTEG.
    OrthoDBi EOG7M98QW.

    Enzyme and pathway databases

    BioCyci YEAST:G3O-31873-MONOMER.

    Miscellaneous databases

    NextBioi 974906.
    PROi P36009.

    Gene expression databases

    Genevestigatori P36009.

    Family and domain databases

    Gene3Di 3.40.50.300. 2 hits.
    InterProi IPR011545. DNA/RNA_helicase_DEAD/DEAH_N.
    IPR002464. DNA/RNA_helicase_DEAH_CS.
    IPR011709. DUF1605.
    IPR007502. Helicase-assoc_dom.
    IPR014001. Helicase_ATP-bd.
    IPR001650. Helicase_C.
    IPR027417. P-loop_NTPase.
    [Graphical view ]
    Pfami PF00270. DEAD. 1 hit.
    PF04408. HA2. 1 hit.
    PF00271. Helicase_C. 1 hit.
    PF07717. OB_NTP_bind. 1 hit.
    [Graphical view ]
    SMARTi SM00487. DEXDc. 1 hit.
    SM00847. HA2. 1 hit.
    SM00490. HELICc. 1 hit.
    [Graphical view ]
    SUPFAMi SSF52540. SSF52540. 1 hit.
    PROSITEi PS00690. DEAH_ATP_HELICASE. 1 hit.
    PS51192. HELICASE_ATP_BIND_1. 1 hit.
    PS51194. HELICASE_CTER. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "JA2: a DEAH family RNA-helicase-like gene from Saccharomyces cerevisiae."
      Arenas J.E., Messenger J.
      Submitted (JUL-1997) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    2. "A clone isolated from a Clontech HeLa cDNA library has high homology to yeast DEAH helicases and hybridizes to Northern and Southern blots from yeast but not from HeLa."
      Raju V.S., Datta P.K., Beales M.H., Ghoshal K., Jacob S.T.
      Submitted (NOV-1993) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    3. "Sequence analysis of a 10 kb fragment of yeast chromosome XI identifies the SMY1 locus and reveals sequences related to a pre-mRNA splicing factor and vacuolar ATPase subunit C plus a number of unidentified open reading frames."
      James C.M., Gent M.E., Indge K.J., Oliver S.G.
      Yeast 10:247-255(1994) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    4. "Complete DNA sequence of yeast chromosome XI."
      Dujon B., Alexandraki D., Andre B., Ansorge W., Baladron V., Ballesta J.P.G., Banrevi A., Bolle P.-A., Bolotin-Fukuhara M., Bossier P., Bou G., Boyer J., Buitrago M.J., Cheret G., Colleaux L., Daignan-Fornier B., del Rey F., Dion C.
      , Domdey H., Duesterhoeft A., Duesterhus S., Entian K.-D., Erfle H., Esteban P.F., Feldmann H., Fernandes L., Fobo G.M., Fritz C., Fukuhara H., Gabel C., Gaillon L., Garcia-Cantalejo J.M., Garcia-Ramirez J.J., Gent M.E., Ghazvini M., Goffeau A., Gonzalez A., Grothues D., Guerreiro P., Hegemann J.H., Hewitt N., Hilger F., Hollenberg C.P., Horaitis O., Indge K.J., Jacquier A., James C.M., Jauniaux J.-C., Jimenez A., Keuchel H., Kirchrath L., Kleine K., Koetter P., Legrain P., Liebl S., Louis E.J., Maia e Silva A., Marck C., Monnier A.-L., Moestl D., Mueller S., Obermaier B., Oliver S.G., Pallier C., Pascolo S., Pfeiffer F., Philippsen P., Planta R.J., Pohl F.M., Pohl T.M., Poehlmann R., Portetelle D., Purnelle B., Puzos V., Ramezani Rad M., Rasmussen S.W., Remacha M.A., Revuelta J.L., Richard G.-F., Rieger M., Rodrigues-Pousada C., Rose M., Rupp T., Santos M.A., Schwager C., Sensen C., Skala J., Soares H., Sor F., Stegemann J., Tettelin H., Thierry A., Tzermia M., Urrestarazu L.A., van Dyck L., van Vliet-Reedijk J.C., Valens M., Vandenbol M., Vilela C., Vissers S., von Wettstein D., Voss H., Wiemann S., Xu G., Zimmermann J., Haasemann M., Becker I., Mewes H.-W.
      Nature 369:371-378(1994) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 204508 / S288c.
    5. Cited for: GENOME REANNOTATION.
      Strain: ATCC 204508 / S288c.
    6. "Dhr1p, a putative DEAH-Box RNA helicase, is associated with the box C+D snoRNP U3."
      Colley A., Beggs J.D., Tollervey D., Lafontaine D.L.J.
      Mol. Cell. Biol. 20:7238-7246(2000) [PubMed] [Europe PMC] [Abstract]
      Cited for: CHARACTERIZATION.
    7. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
    8. "The nucleolar protein Nop19p interacts preferentially with Utp25p and Dhr2p and is essential for the production of the 40S ribosomal subunit in Saccharomyces cerevisiae."
      Choque E., Marcellin M., Burlet-Schiltz O., Gadal O., Dez C.
      RNA Biol. 8:1158-1172(2011) [PubMed] [Europe PMC] [Abstract]
      Cited for: INTERACTION WITH NOP19.

    Entry informationi

    Entry nameiDHR2_YEAST
    AccessioniPrimary (citable) accession number: P36009
    Secondary accession number(s): D6VXK9, Q07040
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: June 1, 1994
    Last sequence update: June 1, 1994
    Last modified: October 1, 2014
    This is version 137 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programFungal Protein Annotation Program

    Miscellaneousi

    Miscellaneous

    Present with 721 molecules/cell in log phase SD medium.1 Publication

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families
    2. Yeast
      Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
    3. Yeast chromosome XI
      Yeast (Saccharomyces cerevisiae) chromosome XI: entries and gene names

    External Data

    Dasty 3