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P36009 (DHR2_YEAST) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 135. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Probable ATP-dependent RNA helicase DHR2

EC=3.6.4.13
Alternative name(s):
DEAH box RNA helicase DHR2
Helicase JA2
Gene names
Name:DHR2
Ordered Locus Names:YKL078W
ORF Names:YKL408
OrganismSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]
Taxonomic identifier559292 [NCBI]
Taxonomic lineageEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces

Protein attributes

Sequence length735 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Probable ATP-binding RNA helicase. Required for 18S rRNA synthesis.

Catalytic activity

ATP + H2O = ADP + phosphate.

Subunit structure

Interacts with NOP19. Ref.8

Subcellular location

Nucleusnucleolus.

Miscellaneous

Present with 721 molecules/cell in log phase SD medium.

Sequence similarities

Belongs to the DEAD box helicase family. DEAH subfamily.

Contains 1 helicase ATP-binding domain.

Contains 1 helicase C-terminal domain.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 735735Probable ATP-dependent RNA helicase DHR2
PRO_0000055163

Regions

Domain91 – 257167Helicase ATP-binding
Domain262 – 456195Helicase C-terminal
Nucleotide binding104 – 1118ATP By similarity
Motif203 – 2064DEAH box

Experimental info

Sequence conflict281T → M in AAA34986. Ref.2
Sequence conflict80 – 9819TLPVY…IESNP → HFRLPTQARNNVIYSKQS in AAA34986. Ref.2
Sequence conflict277 – 28812DAVIR…IQINQ → ALSSGVYTNKP in AAA34986. Ref.2
Sequence conflict3391A → P in AAA34986. Ref.2

Sequences

Sequence LengthMass (Da)Tools
P36009 [UniParc].

Last modified June 1, 1994. Version 1.
Checksum: AAFA78B5F6215D89

FASTA73582,713
        10         20         30         40         50         60 
MAANSNSRVA SNHTSKKQKV RRNIHPFTNN TRIKRASKIV KFNDSGEGDH VSDQRSNKEN 

        70         80         90        100        110        120 
VLTYKSLKSR ASDLLKMRET LPVYQHKREI MSYIESNPVT VLIGETGSGK STQIPQFVLE 

       130        140        150        160        170        180 
KLYDTKKHGS IAVTQPRRVA AINLATRVAQ EHGCKLGEQV GYSVRFDNTT TTRTRLKYLT 

       190        200        210        220        230        240 
DGMLLRELMM NSDLREYSVI VIDEAHERTV LTDLILGFLK SLIQGPRPDL RIIVMSATLQ 

       250        260        270        280        290        300 
AEKFSEFFNN APILFVEGRK FDVKQYYLKA PTDDIVDAVI RCCIQINQGE ELGDILCFLP 

       310        320        330        340        350        360 
GQEEIDKAVT IMEKIAKYVS DEAPVPLIVP YPLYAALPAV QQSLVFAPIK GFKRKVVFST 

       370        380        390        400        410        420 
NIAETSVTIS GVKFVVDSGL RKVKVWRHQL GLATLLTVPI SQASAMQRSG RAGRESEGKS 

       430        440        450        460        470        480 
FRLYCESDYV KLPKQSEPEI ARSDVTSPVL MLKRYGVDDL LNWTWFENPG KEAIVMGLQE 

       490        500        510        520        530        540 
LYELGALDTR GKITKRGQQM ALLPLQPHLS SVLIKASEVG CLSQVIDIVS CLSVENLLLN 

       550        560        570        580        590        600 
PSPEERDEVN ERRLSLCNAG KRYGDLIMLK ELFDIYFYEL GKSQDASSER NDWCKGLCIS 

       610        620        630        640        650        660 
IRGFKNVIRV RDQLRVYCKR LFSSISEEDE ESKKIGEDGE LISKILKCFL TGFIKNTAIG 

       670        680        690        700        710        720 
MPDRSYRTVS TGEPISIHPS SMLFMNKSCP GIMYTEYVFT TKGYARNVSR IELSWLQEVV 

       730 
TNAAAVAKQK VSDSK 

« Hide

References

« Hide 'large scale' references
[1]"JA2: a DEAH family RNA-helicase-like gene from Saccharomyces cerevisiae."
Arenas J.E., Messenger J.
Submitted (JUL-1997) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]"A clone isolated from a Clontech HeLa cDNA library has high homology to yeast DEAH helicases and hybridizes to Northern and Southern blots from yeast but not from HeLa."
Raju V.S., Datta P.K., Beales M.H., Ghoshal K., Jacob S.T.
Submitted (NOV-1993) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[3]"Sequence analysis of a 10 kb fragment of yeast chromosome XI identifies the SMY1 locus and reveals sequences related to a pre-mRNA splicing factor and vacuolar ATPase subunit C plus a number of unidentified open reading frames."
James C.M., Gent M.E., Indge K.J., Oliver S.G.
Yeast 10:247-255(1994) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[4]"Complete DNA sequence of yeast chromosome XI."
Dujon B., Alexandraki D., Andre B., Ansorge W., Baladron V., Ballesta J.P.G., Banrevi A., Bolle P.-A., Bolotin-Fukuhara M., Bossier P., Bou G., Boyer J., Buitrago M.J., Cheret G., Colleaux L., Daignan-Fornier B., del Rey F., Dion C. expand/collapse author list , Domdey H., Duesterhoeft A., Duesterhus S., Entian K.-D., Erfle H., Esteban P.F., Feldmann H., Fernandes L., Fobo G.M., Fritz C., Fukuhara H., Gabel C., Gaillon L., Garcia-Cantalejo J.M., Garcia-Ramirez J.J., Gent M.E., Ghazvini M., Goffeau A., Gonzalez A., Grothues D., Guerreiro P., Hegemann J.H., Hewitt N., Hilger F., Hollenberg C.P., Horaitis O., Indge K.J., Jacquier A., James C.M., Jauniaux J.-C., Jimenez A., Keuchel H., Kirchrath L., Kleine K., Koetter P., Legrain P., Liebl S., Louis E.J., Maia e Silva A., Marck C., Monnier A.-L., Moestl D., Mueller S., Obermaier B., Oliver S.G., Pallier C., Pascolo S., Pfeiffer F., Philippsen P., Planta R.J., Pohl F.M., Pohl T.M., Poehlmann R., Portetelle D., Purnelle B., Puzos V., Ramezani Rad M., Rasmussen S.W., Remacha M.A., Revuelta J.L., Richard G.-F., Rieger M., Rodrigues-Pousada C., Rose M., Rupp T., Santos M.A., Schwager C., Sensen C., Skala J., Soares H., Sor F., Stegemann J., Tettelin H., Thierry A., Tzermia M., Urrestarazu L.A., van Dyck L., van Vliet-Reedijk J.C., Valens M., Vandenbol M., Vilela C., Vissers S., von Wettstein D., Voss H., Wiemann S., Xu G., Zimmermann J., Haasemann M., Becker I., Mewes H.-W.
Nature 369:371-378(1994) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 204508 / S288c.
[5]Saccharomyces Genome Database
Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases
Cited for: GENOME REANNOTATION.
Strain: ATCC 204508 / S288c.
[6]"Dhr1p, a putative DEAH-Box RNA helicase, is associated with the box C+D snoRNP U3."
Colley A., Beggs J.D., Tollervey D., Lafontaine D.L.J.
Mol. Cell. Biol. 20:7238-7246(2000) [PubMed] [Europe PMC] [Abstract]
Cited for: CHARACTERIZATION.
[7]"Global analysis of protein expression in yeast."
Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S.
Nature 425:737-741(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[8]"The nucleolar protein Nop19p interacts preferentially with Utp25p and Dhr2p and is essential for the production of the 40S ribosomal subunit in Saccharomyces cerevisiae."
Choque E., Marcellin M., Burlet-Schiltz O., Gadal O., Dez C.
RNA Biol. 8:1158-1172(2011) [PubMed] [Europe PMC] [Abstract]
Cited for: INTERACTION WITH NOP19.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AF005090 Genomic DNA. Translation: AAB61670.1.
L15328 Genomic DNA. Translation: AAA34986.1.
X75560 Genomic DNA. Translation: CAA53239.1.
Z28078 Genomic DNA. Translation: CAA81915.1.
BK006944 Genomic DNA. Translation: DAA09079.1.
PIRS37903.
RefSeqNP_012845.1. NM_001179644.1.

3D structure databases

ProteinModelPortalP36009.
SMRP36009. Positions 58-719.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid34054. 33 interactions.
DIPDIP-6424N.
IntActP36009. 2 interactions.
MINTMINT-677951.
STRING4932.YKL078W.

Proteomic databases

PaxDbP36009.
PeptideAtlasP36009.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblFungiYKL078W; YKL078W; YKL078W.
GeneID853784.
KEGGsce:YKL078W.

Organism-specific databases

CYGDYKL078w.
SGDS000001561. DHR2.

Phylogenomic databases

eggNOGCOG1643.
GeneTreeENSGT00750000118132.
HOGENOMHOG000175261.
KOK14781.
OMAIKFLTEG.
OrthoDBEOG7M98QW.

Enzyme and pathway databases

BioCycYEAST:G3O-31873-MONOMER.

Gene expression databases

GenevestigatorP36009.

Family and domain databases

Gene3D3.40.50.300. 2 hits.
InterProIPR011545. DNA/RNA_helicase_DEAD/DEAH_N.
IPR002464. DNA/RNA_helicase_DEAH_CS.
IPR011709. DUF1605.
IPR007502. Helicase-assoc_dom.
IPR014001. Helicase_ATP-bd.
IPR001650. Helicase_C.
IPR027417. P-loop_NTPase.
[Graphical view]
PfamPF00270. DEAD. 1 hit.
PF04408. HA2. 1 hit.
PF00271. Helicase_C. 1 hit.
PF07717. OB_NTP_bind. 1 hit.
[Graphical view]
SMARTSM00487. DEXDc. 1 hit.
SM00847. HA2. 1 hit.
SM00490. HELICc. 1 hit.
[Graphical view]
SUPFAMSSF52540. SSF52540. 1 hit.
PROSITEPS00690. DEAH_ATP_HELICASE. 1 hit.
PS51192. HELICASE_ATP_BIND_1. 1 hit.
PS51194. HELICASE_CTER. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio974906.
PROP36009.

Entry information

Entry nameDHR2_YEAST
AccessionPrimary (citable) accession number: P36009
Secondary accession number(s): D6VXK9, Q07040
Entry history
Integrated into UniProtKB/Swiss-Prot: June 1, 1994
Last sequence update: June 1, 1994
Last modified: April 16, 2014
This is version 135 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Relevant documents

Yeast chromosome XI

Yeast (Saccharomyces cerevisiae) chromosome XI: entries and gene names

Yeast

Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD

SIMILARITY comments

Index of protein domains and families