P36008 (EF1G2_YEAST) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Elongation factor 1-gamma 2
Short name=EF-1-gamma 2

Alternative name(s):
Eukaryotic elongation factor 1Bgamma 2
Short name=eEF1Bgamma 2
Translation elongation factor 1B gamma 2

Gene names

Name:	TEF4
Synonyms:	EFC1
Ordered Locus Names:	YKL081W

Organism

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)

Taxonomic identifier

559292 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces

Protein attributes

Sequence length	412 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Subunit of the eukaryotic elongation factor 1 complex (eEF1). Probably plays a role in anchoring the complex to other cellular components By similarity.
Pathway	Protein biosynthesis; polypeptide chain elongation.
Subunit structure	The eukaryotic elongation factor 1 complex (eEF1) is probably a heterohexamer. Two trimeric complexes, each composed of eEF1A (TEF1 or TEF2), eEF1Balpha (EFB1) and eEF1Bgamma (CAM1 or TEF4), are probably dimerized via the eF1Bgamma subunits. The eEF1B subcomplex with the GEF activity is formed of eEF1Balpha and eEF1Bgamma. TEF4 interacts with EFB1 By similarity.
Subcellular location	Cytoplasm Ref.5.
Miscellaneous	Present with 102130 molecules/cell in log phase SD medium. Ref.6
Sequence similarities	Contains 1 EF-1-gamma C-terminal domain. Contains 1 GST C-terminal domain. Contains 1 GST N-terminal domain.

Ontologies

Keywords
Biological process	Protein biosynthesis
Cellular component	Cytoplasm
Molecular function	Elongation factor
PTM	Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Cellular component	eukaryotic translation elongation factor 1 complex Inferred from electronic annotation. Source: InterPro mitochondrion Inferred from direct assay. Source: SGD ribosome Traceable author statement. Source: SGD
Molecular function	protein binding Inferred from physical interaction. Source: IntAct translation elongation factor activity Traceable author statement. Source: SGD
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
BUD27	P43573	2	EBI-6329,EBI-22787

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 412	412	Elongation factor 1-gamma 2		PRO_0000208832
Regions
	Domain	1 – 77	77	GST N-terminal
	Domain	86 – 217	132	GST C-terminal
	Domain	251 – 412	162	EF-1-gamma C-terminal
Amino acid modifications
	Modified residue	85	1	Phosphoserine Ref.7
	Modified residue	215	1	Phosphothreonine Ref.7
Experimental info
	Sequence conflict	8	1	I → L in AAA21473. Ref.1
	Sequence conflict	347	1	Missing in AAA21473. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	P36008 [UniParc]. Last modified June 1, 1994. Version 1. Checksum: 42F1B5B25EF66189	FASTA	412	46,520
	10 20 30 40 50 60 MSQGTLYINR SPRNYASEAL ISYFKLDVKI VDLEQSSEFA SLFPLKQAPA FLGPKGLKLT 70 80 90 100 110 120 EALAIQFYLA NQVADEKERA RLLGSDVIEK SQILRWASLA NSDVMSNIAR PFLSFKGLIP 130 140 150 160 170 180 YNKKDVDACF VKIDNLAAVF DARLRDYTFV ATENISLGDL HAAGSWAFGL ATILGPEWRA 190 200 210 220 230 240 KHPHLMRWFN TVAASPIVKT PFAEVKLAEK ALTYTPPKKQ KAEKPKAEKS KAEKKKDEAK 250 260 270 280 290 300 PADDAAPAKK PKHPLEALGK STFVLDDWKR KYSNDDTRPV ALPWFWEHYN PEEYSIWKVG 310 320 330 340 350 360 YKYNDELTLT FMSNNLVGGF FNRLSASTKY MFGCLVVYGE NNNNGIVGAV MVRGQDFAPA 370 380 390 400 410 FDVAPDWESY EYTKLDPTKE EDKEFVNNMW AWDKPVVVNG EDKEIVDGKV LK

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Multiple genes encode the translation elongation factor EF-1 gamma in Saccharomyces cerevisiae." Kinzy T.G., Ripmaster T.L., Woolford J.L. Jr. Nucleic Acids Res. 22:2703-2707(1994) [PubMed: 8041634] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"Complete DNA sequence of yeast chromosome XI." Dujon B., Alexandraki D., Andre B., Ansorge W., Baladron V., Ballesta J.P.G., Banrevi A., Bolle P.-A., Bolotin-Fukuhara M., Bossier P., Bou G., Boyer J., Buitrago M.J., Cheret G., Colleaux L., Daignan-Fornier B., del Rey F., Dion C. , Domdey H., Duesterhoeft A., Duesterhus S., Entian K.-D., Erfle H., Esteban P.F., Feldmann H., Fernandes L., Fobo G.M., Fritz C., Fukuhara H., Gabel C., Gaillon L., Garcia-Cantalejo J.M., Garcia-Ramirez J.J., Gent M.E., Ghazvini M., Goffeau A., Gonzalez A., Grothues D., Guerreiro P., Hegemann J.H., Hewitt N., Hilger F., Hollenberg C.P., Horaitis O., Indge K.J., Jacquier A., James C.M., Jauniaux J.-C., Jimenez A., Keuchel H., Kirchrath L., Kleine K., Koetter P., Legrain P., Liebl S., Louis E.J., Maia e Silva A., Marck C., Monnier A.-L., Moestl D., Mueller S., Obermaier B., Oliver S.G., Pallier C., Pascolo S., Pfeiffer F., Philippsen P., Planta R.J., Pohl F.M., Pohl T.M., Poehlmann R., Portetelle D., Purnelle B., Puzos V., Ramezani Rad M., Rasmussen S.W., Remacha M.A., Revuelta J.L., Richard G.-F., Rieger M., Rodrigues-Pousada C., Rose M., Rupp T., Santos M.A., Schwager C., Sensen C., Skala J., Soares H., Sor F., Stegemann J., Tettelin H., Thierry A., Tzermia M., Urrestarazu L.A., van Dyck L., van Vliet-Reedijk J.C., Valens M., Vandenbol M., Vilela C., Vissers S., von Wettstein D., Voss H., Wiemann S., Xu G., Zimmermann J., Haasemann M., Becker I., Mewes H.-W. Nature 369:371-378(1994) [PubMed: 8196765] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 96604 / S288c / FY1679.
[3]	Saccharomyces Genome Database Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: ATCC 204508 / S288c.
[4]	"Cloning and mutational analysis of the gene encoding subunit C of yeast vacuolar H(+)-ATPase." Beltran C., Kopecky J., Pan Y.-C.E., Nelson H., Nelson N. J. Biol. Chem. 267:774-779(1992) [PubMed: 1730668] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 257-412.
[5]	"Global analysis of protein localization in budding yeast." Huh W.-K., Falvo J.V., Gerke L.C., Carroll A.S., Howson R.W., Weissman J.S., O'Shea E.K. Nature 425:686-691(2003) [PubMed: 14562095] [Abstract] Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
[6]	"Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed: 14562106] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[7]	"A multidimensional chromatography technology for in-depth phosphoproteome analysis." Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H. Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed: 18407956] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-85 AND THR-215, MASS SPECTROMETRY.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	L01880 Genomic DNA. Translation: AAA21473.1. Z28081 Genomic DNA. Translation: CAA81919.1. Z28080 Genomic DNA. Translation: CAA81918.1. M77143 Genomic DNA. No translation available. BK006944 Genomic DNA. Translation: DAA09076.1.
PIR	S37906.
RefSeq	NP_012842.1. NM_001179647.1.
3D structure databases
ProteinModelPortal	P36008.
SMR	P36008. Positions 1-213, 251-412.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-6386N.
IntAct	P36008. 54 interactions.
MINT	MINT-621528.
STRING	P36008.
Proteomic databases
PeptideAtlas	P36008.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblFungi	YKL081W; YKL081W; YKL081W.
GeneID	853781.
KEGG	sce:YKL081W.
NMPDR	fig|4932.3.peg.3826.
Organism-specific databases
CYGD	YKL081w.
SGD	S000001564. TEF4.
Phylogenomic databases
eggNOG	fuNOG05502.
GeneTree	EFGT00050000001375.
HOGENOM	HBG436026.
OMA	DISVACN.
OrthoDB	EOG43FM66.
Gene expression databases
ArrayExpress	P36008.
Genevestigator	P36008.
GermOnline	YKL081W. Saccharomyces cerevisiae.
Family and domain databases
InterPro	IPR010987. Glutathione-S-Trfase_C-like. IPR004045. Glutathione_S-Trfase_N. IPR017933. Glutathione_S_Trfase/Cl_chnl_C. IPR004046. GST_C. IPR012336. Thioredoxin-like_fold. IPR001662. Transl_elong_EF1_G_con. [Graphical view]
Gene3D	G3DSA:1.20.1050.10. GST_C_like. 1 hit. G3DSA:3.40.30.10. Thioredoxin_fold. 1 hit. G3DSA:3.30.70.1010. Transl_elong_EF1_G_con. 1 hit.
KO	K03233.
Pfam	PF00647. EF1G. 1 hit. PF00043. GST_C. 1 hit. PF02798. GST_N. 1 hit. [Graphical view]
SUPFAM	SSF47616. GST_C_like. 1 hit. SSF52833. Thiordxn-like_fd. 1 hit. SSF89942. Transl_elong_EF1_G_con. 1 hit.
PROSITE	PS50040. EF1G_C. 1 hit. PS50405. GST_CTER. 1 hit. PS50404. GST_NTER. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	974897.

Entry information

Entry name

EF1G2_YEAST

Accession

Primary (citable) accession number: P36008
Secondary accession number(s): D6VXK6

Entry history

Integrated into UniProtKB/Swiss-Prot:	June 1, 1994
Last sequence update:	June 1, 1994
Last modified:	December 14, 2011
This is version 103 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program