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P36008 (EF1G2_YEAST) Reviewed, UniProtKB/Swiss-Prot

Last modified June 11, 2014. Version 125. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (4) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Elongation factor 1-gamma 2

Short name=EF-1-gamma 2
Alternative name(s):
Eukaryotic elongation factor 1Bgamma 2
Short name=eEF1Bgamma 2
Translation elongation factor 1B gamma 2
Gene names
Name:TEF4
Synonyms:EFC1
Ordered Locus Names:YKL081W
OrganismSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]
Taxonomic identifier559292 [NCBI]
Taxonomic lineageEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces

Protein attributes

Sequence length412 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Subunit of the eukaryotic elongation factor 1 complex (eEF1). Probably plays a role in anchoring the complex to other cellular components By similarity.

Pathway

Protein biosynthesis; polypeptide chain elongation.

Subunit structure

The eukaryotic elongation factor 1 complex (eEF1) is probably a heterohexamer. Two trimeric complexes, each composed of eEF1A (TEF1 or TEF2), eEF1Balpha (EFB1) and eEF1Bgamma (CAM1 or TEF4), are probably dimerized via the eF1Bgamma subunits. The eEF1B subcomplex with the GEF activity is formed of eEF1Balpha and eEF1Bgamma. TEF4 interacts with EFB1 By similarity.

Subcellular location

Cytoplasm Ref.5.

Miscellaneous

Present with 102130 molecules/cell in log phase SD medium.

Sequence similarities

Contains 1 EF-1-gamma C-terminal domain.

Contains 1 GST C-terminal domain.

Contains 1 GST N-terminal domain.

Binary interactions

With

Entry

#Exp.

IntAct

Notes

BUD27P435733EBI-6329,EBI-22787

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Initiator methionine11Removed Ref.7
Chain2 – 412411Elongation factor 1-gamma 2
PRO_0000208832

Regions

Domain2 – 7776GST N-terminal
Domain86 – 217132GST C-terminal
Domain251 – 412162EF-1-gamma C-terminal

Amino acid modifications

Modified residue21N-acetylserine Ref.7

Experimental info

Sequence conflict81I → L in AAA21473. Ref.1
Sequence conflict3471Missing in AAA21473. Ref.1

Sequences

Sequence LengthMass (Da)Tools
P36008 [UniParc].

Last modified June 1, 1994. Version 1.
Checksum: 42F1B5B25EF66189

FASTA41246,520
        10         20         30         40         50         60 
MSQGTLYINR SPRNYASEAL ISYFKLDVKI VDLEQSSEFA SLFPLKQAPA FLGPKGLKLT 

        70         80         90        100        110        120 
EALAIQFYLA NQVADEKERA RLLGSDVIEK SQILRWASLA NSDVMSNIAR PFLSFKGLIP 

       130        140        150        160        170        180 
YNKKDVDACF VKIDNLAAVF DARLRDYTFV ATENISLGDL HAAGSWAFGL ATILGPEWRA 

       190        200        210        220        230        240 
KHPHLMRWFN TVAASPIVKT PFAEVKLAEK ALTYTPPKKQ KAEKPKAEKS KAEKKKDEAK 

       250        260        270        280        290        300 
PADDAAPAKK PKHPLEALGK STFVLDDWKR KYSNDDTRPV ALPWFWEHYN PEEYSIWKVG 

       310        320        330        340        350        360 
YKYNDELTLT FMSNNLVGGF FNRLSASTKY MFGCLVVYGE NNNNGIVGAV MVRGQDFAPA 

       370        380        390        400        410 
FDVAPDWESY EYTKLDPTKE EDKEFVNNMW AWDKPVVVNG EDKEIVDGKV LK 

« Hide

References

« Hide 'large scale' references
[1]"Multiple genes encode the translation elongation factor EF-1 gamma in Saccharomyces cerevisiae."
Kinzy T.G., Ripmaster T.L., Woolford J.L. Jr.
Nucleic Acids Res. 22:2703-2707(1994) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]"Complete DNA sequence of yeast chromosome XI."
Dujon B., Alexandraki D., Andre B., Ansorge W., Baladron V., Ballesta J.P.G., Banrevi A., Bolle P.-A., Bolotin-Fukuhara M., Bossier P., Bou G., Boyer J., Buitrago M.J., Cheret G., Colleaux L., Daignan-Fornier B., del Rey F., Dion C. expand/collapse author list , Domdey H., Duesterhoeft A., Duesterhus S., Entian K.-D., Erfle H., Esteban P.F., Feldmann H., Fernandes L., Fobo G.M., Fritz C., Fukuhara H., Gabel C., Gaillon L., Garcia-Cantalejo J.M., Garcia-Ramirez J.J., Gent M.E., Ghazvini M., Goffeau A., Gonzalez A., Grothues D., Guerreiro P., Hegemann J.H., Hewitt N., Hilger F., Hollenberg C.P., Horaitis O., Indge K.J., Jacquier A., James C.M., Jauniaux J.-C., Jimenez A., Keuchel H., Kirchrath L., Kleine K., Koetter P., Legrain P., Liebl S., Louis E.J., Maia e Silva A., Marck C., Monnier A.-L., Moestl D., Mueller S., Obermaier B., Oliver S.G., Pallier C., Pascolo S., Pfeiffer F., Philippsen P., Planta R.J., Pohl F.M., Pohl T.M., Poehlmann R., Portetelle D., Purnelle B., Puzos V., Ramezani Rad M., Rasmussen S.W., Remacha M.A., Revuelta J.L., Richard G.-F., Rieger M., Rodrigues-Pousada C., Rose M., Rupp T., Santos M.A., Schwager C., Sensen C., Skala J., Soares H., Sor F., Stegemann J., Tettelin H., Thierry A., Tzermia M., Urrestarazu L.A., van Dyck L., van Vliet-Reedijk J.C., Valens M., Vandenbol M., Vilela C., Vissers S., von Wettstein D., Voss H., Wiemann S., Xu G., Zimmermann J., Haasemann M., Becker I., Mewes H.-W.
Nature 369:371-378(1994) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 204508 / S288c.
[3]"The reference genome sequence of Saccharomyces cerevisiae: Then and now."
Engel S.R., Dietrich F.S., Fisk D.G., Binkley G., Balakrishnan R., Costanzo M.C., Dwight S.S., Hitz B.C., Karra K., Nash R.S., Weng S., Wong E.D., Lloyd P., Skrzypek M.S., Miyasato S.R., Simison M., Cherry J.M.
G3 (Bethesda) 4:389-398(2014) [PubMed] [Europe PMC] [Abstract]
Cited for: GENOME REANNOTATION.
Strain: ATCC 204508 / S288c.
[4]"Cloning and mutational analysis of the gene encoding subunit C of yeast vacuolar H(+)-ATPase."
Beltran C., Kopecky J., Pan Y.-C.E., Nelson H., Nelson N.
J. Biol. Chem. 267:774-779(1992) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 257-412.
[5]"Global analysis of protein localization in budding yeast."
Huh W.-K., Falvo J.V., Gerke L.C., Carroll A.S., Howson R.W., Weissman J.S., O'Shea E.K.
Nature 425:686-691(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
[6]"Global analysis of protein expression in yeast."
Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S.
Nature 425:737-741(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[7]"N-terminal acetylome analyses and functional insights of the N-terminal acetyltransferase NatB."
Van Damme P., Lasa M., Polevoda B., Gazquez C., Elosegui-Artola A., Kim D.S., De Juan-Pardo E., Demeyer K., Hole K., Larrea E., Timmerman E., Prieto J., Arnesen T., Sherman F., Gevaert K., Aldabe R.
Proc. Natl. Acad. Sci. U.S.A. 109:12449-12454(2012) [PubMed] [Europe PMC] [Abstract]
Cited for: ACETYLATION [LARGE SCALE ANALYSIS] AT SER-2, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS], CLEAVAGE OF INITIATOR METHIONINE [LARGE SCALE ANALYSIS].
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
L01880 Genomic DNA. Translation: AAA21473.1.
Z28081 Genomic DNA. Translation: CAA81919.1.
Z28080 Genomic DNA. Translation: CAA81918.1.
M77143 Genomic DNA. No translation available.
BK006944 Genomic DNA. Translation: DAA09076.1.
PIRS37906.
RefSeqNP_012842.1. NM_001179647.1.

3D structure databases

ProteinModelPortalP36008.
SMRP36008. Positions 1-218, 252-393.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid34051. 183 interactions.
DIPDIP-6386N.
IntActP36008. 46 interactions.
MINTMINT-621528.
STRING4932.YKL081W.

Proteomic databases

MaxQBP36008.
PaxDbP36008.
PeptideAtlasP36008.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblFungiYKL081W; YKL081W; YKL081W.
GeneID853781.
KEGGsce:YKL081W.

Organism-specific databases

CYGDYKL081w.
SGDS000001564. TEF4.

Phylogenomic databases

eggNOGCOG0625.
GeneTreeENSGT00390000007552.
HOGENOMHOG000201196.
KOK03233.
OMADIVTICN.
OrthoDBEOG7RBZK2.

Enzyme and pathway databases

BioCycYEAST:G3O-31876-MONOMER.
UniPathwayUPA00345.

Gene expression databases

GenevestigatorP36008.

Family and domain databases

Gene3D1.20.1050.10. 1 hit.
3.30.70.1010. 1 hit.
3.40.30.10. 1 hit.
InterProIPR010987. Glutathione-S-Trfase_C-like.
IPR004045. Glutathione_S-Trfase_N.
IPR004046. GST_C.
IPR012336. Thioredoxin-like_fold.
IPR001662. Transl_elong_EF1_G_con.
[Graphical view]
PfamPF00647. EF1G. 1 hit.
PF00043. GST_C. 1 hit.
PF02798. GST_N. 1 hit.
[Graphical view]
SUPFAMSSF47616. SSF47616. 1 hit.
SSF52833. SSF52833. 1 hit.
SSF89942. SSF89942. 1 hit.
PROSITEPS50040. EF1G_C. 1 hit.
PS50405. GST_CTER. 1 hit.
PS50404. GST_NTER. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio974897.

Entry information

Entry nameEF1G2_YEAST
AccessionPrimary (citable) accession number: P36008
Secondary accession number(s): D6VXK6
Entry history
Integrated into UniProtKB/Swiss-Prot: June 1, 1994
Last sequence update: June 1, 1994
Last modified: June 11, 2014
This is version 125 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Relevant documents

Yeast chromosome XI

Yeast (Saccharomyces cerevisiae) chromosome XI: entries and gene names

Yeast

Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways