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P36008

- EF1G2_YEAST

UniProt

P36008 - EF1G2_YEAST

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Protein

Elongation factor 1-gamma 2

Gene

TEF4

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli

Functioni

Subunit of the eukaryotic elongation factor 1 complex (eEF1). Probably plays a role in anchoring the complex to other cellular components (By similarity).By similarity

Pathwayi

GO - Molecular functioni

  1. translation elongation factor activity Source: SGD

GO - Biological processi

  1. translational elongation Source: SGD
Complete GO annotation...

Keywords - Molecular functioni

Elongation factor

Keywords - Biological processi

Protein biosynthesis

Enzyme and pathway databases

BioCyciYEAST:G3O-31876-MONOMER.
ReactomeiREACT_247749. Eukaryotic Translation Elongation.
UniPathwayiUPA00345.

Names & Taxonomyi

Protein namesi
Recommended name:
Elongation factor 1-gamma 2
Short name:
EF-1-gamma 2
Alternative name(s):
Eukaryotic elongation factor 1Bgamma 2
Short name:
eEF1Bgamma 2
Translation elongation factor 1B gamma 2
Gene namesi
Name:TEF4
Synonyms:EFC1
Ordered Locus Names:YKL081W
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
ProteomesiUP000002311: Chromosome XI

Organism-specific databases

CYGDiYKL081w.
SGDiS000001564. TEF4.

Subcellular locationi

Cytoplasm 1 Publication

GO - Cellular componenti

  1. cytoplasmic stress granule Source: SGD
  2. eukaryotic translation elongation factor 1 complex Source: SGD
  3. ribosome Source: SGD
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methioninei1 – 11Removed1 Publication
Chaini2 – 412411Elongation factor 1-gamma 2PRO_0000208832Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei2 – 21N-acetylserine1 Publication

Keywords - PTMi

Acetylation

Proteomic databases

MaxQBiP36008.
PaxDbiP36008.
PeptideAtlasiP36008.

Expressioni

Gene expression databases

GenevestigatoriP36008.

Interactioni

Subunit structurei

The eukaryotic elongation factor 1 complex (eEF1) is probably a heterohexamer. Two trimeric complexes, each composed of eEF1A (TEF1 or TEF2), eEF1Balpha (EFB1) and eEF1Bgamma (CAM1 or TEF4), are probably dimerized via the eF1Bgamma subunits. The eEF1B subcomplex with the GEF activity is formed of eEF1Balpha and eEF1Bgamma. TEF4 interacts with EFB1 (By similarity).By similarity

Binary interactionsi

WithEntry#Exp.IntActNotes
BUD27P435733EBI-6329,EBI-22787

Protein-protein interaction databases

BioGridi34051. 185 interactions.
DIPiDIP-6386N.
IntActiP36008. 46 interactions.
MINTiMINT-621528.
STRINGi4932.YKL081W.

Structurei

3D structure databases

ProteinModelPortaliP36008.
SMRiP36008. Positions 1-218, 252-393.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini2 – 7776GST N-terminalAdd
BLAST
Domaini86 – 217132GST C-terminalAdd
BLAST
Domaini251 – 412162EF-1-gamma C-terminalPROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Contains 1 EF-1-gamma C-terminal domain.PROSITE-ProRule annotation
Contains 1 GST C-terminal domain.Curated
Contains 1 GST N-terminal domain.Curated

Phylogenomic databases

eggNOGiCOG0625.
GeneTreeiENSGT00390000007552.
HOGENOMiHOG000201196.
InParanoidiP36008.
KOiK03233.
OMAiDIVTICN.
OrthoDBiEOG7RBZK2.

Family and domain databases

Gene3Di1.20.1050.10. 1 hit.
3.30.70.1010. 1 hit.
3.40.30.10. 1 hit.
InterProiIPR010987. Glutathione-S-Trfase_C-like.
IPR004045. Glutathione_S-Trfase_N.
IPR004046. GST_C.
IPR012336. Thioredoxin-like_fold.
IPR001662. Transl_elong_EF1_G_con.
[Graphical view]
PfamiPF00647. EF1G. 1 hit.
PF00043. GST_C. 1 hit.
PF02798. GST_N. 1 hit.
[Graphical view]
SUPFAMiSSF47616. SSF47616. 1 hit.
SSF52833. SSF52833. 1 hit.
SSF89942. SSF89942. 1 hit.
PROSITEiPS50040. EF1G_C. 1 hit.
PS50405. GST_CTER. 1 hit.
PS50404. GST_NTER. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P36008-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MSQGTLYINR SPRNYASEAL ISYFKLDVKI VDLEQSSEFA SLFPLKQAPA
60 70 80 90 100
FLGPKGLKLT EALAIQFYLA NQVADEKERA RLLGSDVIEK SQILRWASLA
110 120 130 140 150
NSDVMSNIAR PFLSFKGLIP YNKKDVDACF VKIDNLAAVF DARLRDYTFV
160 170 180 190 200
ATENISLGDL HAAGSWAFGL ATILGPEWRA KHPHLMRWFN TVAASPIVKT
210 220 230 240 250
PFAEVKLAEK ALTYTPPKKQ KAEKPKAEKS KAEKKKDEAK PADDAAPAKK
260 270 280 290 300
PKHPLEALGK STFVLDDWKR KYSNDDTRPV ALPWFWEHYN PEEYSIWKVG
310 320 330 340 350
YKYNDELTLT FMSNNLVGGF FNRLSASTKY MFGCLVVYGE NNNNGIVGAV
360 370 380 390 400
MVRGQDFAPA FDVAPDWESY EYTKLDPTKE EDKEFVNNMW AWDKPVVVNG
410
EDKEIVDGKV LK
Length:412
Mass (Da):46,520
Last modified:June 1, 1994 - v1
Checksum:i42F1B5B25EF66189
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti8 – 81I → L in AAA21473. (PubMed:8041634)Curated
Sequence conflicti347 – 3471Missing in AAA21473. (PubMed:8041634)Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L01880 Genomic DNA. Translation: AAA21473.1.
Z28081 Genomic DNA. Translation: CAA81919.1.
Z28080 Genomic DNA. Translation: CAA81918.1.
M77143 Genomic DNA. No translation available.
BK006944 Genomic DNA. Translation: DAA09076.1.
PIRiS37906.
RefSeqiNP_012842.1. NM_001179647.1.

Genome annotation databases

EnsemblFungiiYKL081W; YKL081W; YKL081W.
GeneIDi853781.
KEGGisce:YKL081W.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L01880 Genomic DNA. Translation: AAA21473.1 .
Z28081 Genomic DNA. Translation: CAA81919.1 .
Z28080 Genomic DNA. Translation: CAA81918.1 .
M77143 Genomic DNA. No translation available.
BK006944 Genomic DNA. Translation: DAA09076.1 .
PIRi S37906.
RefSeqi NP_012842.1. NM_001179647.1.

3D structure databases

ProteinModelPortali P36008.
SMRi P36008. Positions 1-218, 252-393.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 34051. 185 interactions.
DIPi DIP-6386N.
IntActi P36008. 46 interactions.
MINTi MINT-621528.
STRINGi 4932.YKL081W.

Proteomic databases

MaxQBi P36008.
PaxDbi P36008.
PeptideAtlasi P36008.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblFungii YKL081W ; YKL081W ; YKL081W .
GeneIDi 853781.
KEGGi sce:YKL081W.

Organism-specific databases

CYGDi YKL081w.
SGDi S000001564. TEF4.

Phylogenomic databases

eggNOGi COG0625.
GeneTreei ENSGT00390000007552.
HOGENOMi HOG000201196.
InParanoidi P36008.
KOi K03233.
OMAi DIVTICN.
OrthoDBi EOG7RBZK2.

Enzyme and pathway databases

UniPathwayi UPA00345 .
BioCyci YEAST:G3O-31876-MONOMER.
Reactomei REACT_247749. Eukaryotic Translation Elongation.

Miscellaneous databases

NextBioi 974897.

Gene expression databases

Genevestigatori P36008.

Family and domain databases

Gene3Di 1.20.1050.10. 1 hit.
3.30.70.1010. 1 hit.
3.40.30.10. 1 hit.
InterProi IPR010987. Glutathione-S-Trfase_C-like.
IPR004045. Glutathione_S-Trfase_N.
IPR004046. GST_C.
IPR012336. Thioredoxin-like_fold.
IPR001662. Transl_elong_EF1_G_con.
[Graphical view ]
Pfami PF00647. EF1G. 1 hit.
PF00043. GST_C. 1 hit.
PF02798. GST_N. 1 hit.
[Graphical view ]
SUPFAMi SSF47616. SSF47616. 1 hit.
SSF52833. SSF52833. 1 hit.
SSF89942. SSF89942. 1 hit.
PROSITEi PS50040. EF1G_C. 1 hit.
PS50405. GST_CTER. 1 hit.
PS50404. GST_NTER. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Multiple genes encode the translation elongation factor EF-1 gamma in Saccharomyces cerevisiae."
    Kinzy T.G., Ripmaster T.L., Woolford J.L. Jr.
    Nucleic Acids Res. 22:2703-2707(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  2. "Complete DNA sequence of yeast chromosome XI."
    Dujon B., Alexandraki D., Andre B., Ansorge W., Baladron V., Ballesta J.P.G., Banrevi A., Bolle P.-A., Bolotin-Fukuhara M., Bossier P., Bou G., Boyer J., Buitrago M.J., Cheret G., Colleaux L., Daignan-Fornier B., del Rey F., Dion C.
    , Domdey H., Duesterhoeft A., Duesterhus S., Entian K.-D., Erfle H., Esteban P.F., Feldmann H., Fernandes L., Fobo G.M., Fritz C., Fukuhara H., Gabel C., Gaillon L., Garcia-Cantalejo J.M., Garcia-Ramirez J.J., Gent M.E., Ghazvini M., Goffeau A., Gonzalez A., Grothues D., Guerreiro P., Hegemann J.H., Hewitt N., Hilger F., Hollenberg C.P., Horaitis O., Indge K.J., Jacquier A., James C.M., Jauniaux J.-C., Jimenez A., Keuchel H., Kirchrath L., Kleine K., Koetter P., Legrain P., Liebl S., Louis E.J., Maia e Silva A., Marck C., Monnier A.-L., Moestl D., Mueller S., Obermaier B., Oliver S.G., Pallier C., Pascolo S., Pfeiffer F., Philippsen P., Planta R.J., Pohl F.M., Pohl T.M., Poehlmann R., Portetelle D., Purnelle B., Puzos V., Ramezani Rad M., Rasmussen S.W., Remacha M.A., Revuelta J.L., Richard G.-F., Rieger M., Rodrigues-Pousada C., Rose M., Rupp T., Santos M.A., Schwager C., Sensen C., Skala J., Soares H., Sor F., Stegemann J., Tettelin H., Thierry A., Tzermia M., Urrestarazu L.A., van Dyck L., van Vliet-Reedijk J.C., Valens M., Vandenbol M., Vilela C., Vissers S., von Wettstein D., Voss H., Wiemann S., Xu G., Zimmermann J., Haasemann M., Becker I., Mewes H.-W.
    Nature 369:371-378(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  3. Cited for: GENOME REANNOTATION.
    Strain: ATCC 204508 / S288c.
  4. "Cloning and mutational analysis of the gene encoding subunit C of yeast vacuolar H(+)-ATPase."
    Beltran C., Kopecky J., Pan Y.-C.E., Nelson H., Nelson N.
    J. Biol. Chem. 267:774-779(1992) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 257-412.
  5. Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
  6. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
  7. Cited for: ACETYLATION [LARGE SCALE ANALYSIS] AT SER-2, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS], CLEAVAGE OF INITIATOR METHIONINE [LARGE SCALE ANALYSIS].

Entry informationi

Entry nameiEF1G2_YEAST
AccessioniPrimary (citable) accession number: P36008
Secondary accession number(s): D6VXK6
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 1, 1994
Last sequence update: June 1, 1994
Last modified: November 26, 2014
This is version 128 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 102130 molecules/cell in log phase SD medium.1 Publication

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families
  3. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  4. Yeast chromosome XI
    Yeast (Saccharomyces cerevisiae) chromosome XI: entries and gene names

External Data

Dasty 3