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P36008

- EF1G2_YEAST

UniProt

P36008 - EF1G2_YEAST

Protein

Elongation factor 1-gamma 2

Gene

TEF4

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 126 (01 Oct 2014)
      Sequence version 1 (01 Jun 1994)
      Previous versions | rss
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    Functioni

    Subunit of the eukaryotic elongation factor 1 complex (eEF1). Probably plays a role in anchoring the complex to other cellular components By similarity.By similarity

    Pathwayi

    GO - Molecular functioni

    1. protein binding Source: IntAct
    2. translation elongation factor activity Source: SGD

    GO - Biological processi

    1. translational elongation Source: SGD

    Keywords - Molecular functioni

    Elongation factor

    Keywords - Biological processi

    Protein biosynthesis

    Enzyme and pathway databases

    BioCyciYEAST:G3O-31876-MONOMER.
    UniPathwayiUPA00345.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Elongation factor 1-gamma 2
    Short name:
    EF-1-gamma 2
    Alternative name(s):
    Eukaryotic elongation factor 1Bgamma 2
    Short name:
    eEF1Bgamma 2
    Translation elongation factor 1B gamma 2
    Gene namesi
    Name:TEF4
    Synonyms:EFC1
    Ordered Locus Names:YKL081W
    OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
    Taxonomic identifieri559292 [NCBI]
    Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
    ProteomesiUP000002311: Chromosome XI

    Organism-specific databases

    CYGDiYKL081w.
    SGDiS000001564. TEF4.

    Subcellular locationi

    Cytoplasm 1 Publication

    GO - Cellular componenti

    1. cytoplasmic stress granule Source: SGD
    2. eukaryotic translation elongation factor 1 complex Source: SGD
    3. ribosome Source: SGD

    Keywords - Cellular componenti

    Cytoplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Initiator methioninei1 – 11Removed1 Publication
    Chaini2 – 412411Elongation factor 1-gamma 2PRO_0000208832Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei2 – 21N-acetylserine1 Publication

    Keywords - PTMi

    Acetylation

    Proteomic databases

    MaxQBiP36008.
    PaxDbiP36008.
    PeptideAtlasiP36008.

    Expressioni

    Gene expression databases

    GenevestigatoriP36008.

    Interactioni

    Subunit structurei

    The eukaryotic elongation factor 1 complex (eEF1) is probably a heterohexamer. Two trimeric complexes, each composed of eEF1A (TEF1 or TEF2), eEF1Balpha (EFB1) and eEF1Bgamma (CAM1 or TEF4), are probably dimerized via the eF1Bgamma subunits. The eEF1B subcomplex with the GEF activity is formed of eEF1Balpha and eEF1Bgamma. TEF4 interacts with EFB1 By similarity.By similarity

    Binary interactionsi

    WithEntry#Exp.IntActNotes
    BUD27P435733EBI-6329,EBI-22787

    Protein-protein interaction databases

    BioGridi34051. 184 interactions.
    DIPiDIP-6386N.
    IntActiP36008. 46 interactions.
    MINTiMINT-621528.
    STRINGi4932.YKL081W.

    Structurei

    3D structure databases

    ProteinModelPortaliP36008.
    SMRiP36008. Positions 1-218, 252-393.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini2 – 7776GST N-terminalAdd
    BLAST
    Domaini86 – 217132GST C-terminalAdd
    BLAST
    Domaini251 – 412162EF-1-gamma C-terminalPROSITE-ProRule annotationAdd
    BLAST

    Sequence similaritiesi

    Contains 1 EF-1-gamma C-terminal domain.PROSITE-ProRule annotation
    Contains 1 GST C-terminal domain.Curated
    Contains 1 GST N-terminal domain.Curated

    Phylogenomic databases

    eggNOGiCOG0625.
    GeneTreeiENSGT00390000007552.
    HOGENOMiHOG000201196.
    KOiK03233.
    OMAiDIVTICN.
    OrthoDBiEOG7RBZK2.

    Family and domain databases

    Gene3Di1.20.1050.10. 1 hit.
    3.30.70.1010. 1 hit.
    3.40.30.10. 1 hit.
    InterProiIPR010987. Glutathione-S-Trfase_C-like.
    IPR004045. Glutathione_S-Trfase_N.
    IPR004046. GST_C.
    IPR012336. Thioredoxin-like_fold.
    IPR001662. Transl_elong_EF1_G_con.
    [Graphical view]
    PfamiPF00647. EF1G. 1 hit.
    PF00043. GST_C. 1 hit.
    PF02798. GST_N. 1 hit.
    [Graphical view]
    SUPFAMiSSF47616. SSF47616. 1 hit.
    SSF52833. SSF52833. 1 hit.
    SSF89942. SSF89942. 1 hit.
    PROSITEiPS50040. EF1G_C. 1 hit.
    PS50405. GST_CTER. 1 hit.
    PS50404. GST_NTER. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    P36008-1 [UniParc]FASTAAdd to Basket

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    MSQGTLYINR SPRNYASEAL ISYFKLDVKI VDLEQSSEFA SLFPLKQAPA    50
    FLGPKGLKLT EALAIQFYLA NQVADEKERA RLLGSDVIEK SQILRWASLA 100
    NSDVMSNIAR PFLSFKGLIP YNKKDVDACF VKIDNLAAVF DARLRDYTFV 150
    ATENISLGDL HAAGSWAFGL ATILGPEWRA KHPHLMRWFN TVAASPIVKT 200
    PFAEVKLAEK ALTYTPPKKQ KAEKPKAEKS KAEKKKDEAK PADDAAPAKK 250
    PKHPLEALGK STFVLDDWKR KYSNDDTRPV ALPWFWEHYN PEEYSIWKVG 300
    YKYNDELTLT FMSNNLVGGF FNRLSASTKY MFGCLVVYGE NNNNGIVGAV 350
    MVRGQDFAPA FDVAPDWESY EYTKLDPTKE EDKEFVNNMW AWDKPVVVNG 400
    EDKEIVDGKV LK 412
    Length:412
    Mass (Da):46,520
    Last modified:June 1, 1994 - v1
    Checksum:i42F1B5B25EF66189
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti8 – 81I → L in AAA21473. (PubMed:8041634)Curated
    Sequence conflicti347 – 3471Missing in AAA21473. (PubMed:8041634)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    L01880 Genomic DNA. Translation: AAA21473.1.
    Z28081 Genomic DNA. Translation: CAA81919.1.
    Z28080 Genomic DNA. Translation: CAA81918.1.
    M77143 Genomic DNA. No translation available.
    BK006944 Genomic DNA. Translation: DAA09076.1.
    PIRiS37906.
    RefSeqiNP_012842.1. NM_001179647.1.

    Genome annotation databases

    EnsemblFungiiYKL081W; YKL081W; YKL081W.
    GeneIDi853781.
    KEGGisce:YKL081W.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    L01880 Genomic DNA. Translation: AAA21473.1 .
    Z28081 Genomic DNA. Translation: CAA81919.1 .
    Z28080 Genomic DNA. Translation: CAA81918.1 .
    M77143 Genomic DNA. No translation available.
    BK006944 Genomic DNA. Translation: DAA09076.1 .
    PIRi S37906.
    RefSeqi NP_012842.1. NM_001179647.1.

    3D structure databases

    ProteinModelPortali P36008.
    SMRi P36008. Positions 1-218, 252-393.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 34051. 184 interactions.
    DIPi DIP-6386N.
    IntActi P36008. 46 interactions.
    MINTi MINT-621528.
    STRINGi 4932.YKL081W.

    Proteomic databases

    MaxQBi P36008.
    PaxDbi P36008.
    PeptideAtlasi P36008.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblFungii YKL081W ; YKL081W ; YKL081W .
    GeneIDi 853781.
    KEGGi sce:YKL081W.

    Organism-specific databases

    CYGDi YKL081w.
    SGDi S000001564. TEF4.

    Phylogenomic databases

    eggNOGi COG0625.
    GeneTreei ENSGT00390000007552.
    HOGENOMi HOG000201196.
    KOi K03233.
    OMAi DIVTICN.
    OrthoDBi EOG7RBZK2.

    Enzyme and pathway databases

    UniPathwayi UPA00345 .
    BioCyci YEAST:G3O-31876-MONOMER.

    Miscellaneous databases

    NextBioi 974897.

    Gene expression databases

    Genevestigatori P36008.

    Family and domain databases

    Gene3Di 1.20.1050.10. 1 hit.
    3.30.70.1010. 1 hit.
    3.40.30.10. 1 hit.
    InterProi IPR010987. Glutathione-S-Trfase_C-like.
    IPR004045. Glutathione_S-Trfase_N.
    IPR004046. GST_C.
    IPR012336. Thioredoxin-like_fold.
    IPR001662. Transl_elong_EF1_G_con.
    [Graphical view ]
    Pfami PF00647. EF1G. 1 hit.
    PF00043. GST_C. 1 hit.
    PF02798. GST_N. 1 hit.
    [Graphical view ]
    SUPFAMi SSF47616. SSF47616. 1 hit.
    SSF52833. SSF52833. 1 hit.
    SSF89942. SSF89942. 1 hit.
    PROSITEi PS50040. EF1G_C. 1 hit.
    PS50405. GST_CTER. 1 hit.
    PS50404. GST_NTER. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Multiple genes encode the translation elongation factor EF-1 gamma in Saccharomyces cerevisiae."
      Kinzy T.G., Ripmaster T.L., Woolford J.L. Jr.
      Nucleic Acids Res. 22:2703-2707(1994) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    2. "Complete DNA sequence of yeast chromosome XI."
      Dujon B., Alexandraki D., Andre B., Ansorge W., Baladron V., Ballesta J.P.G., Banrevi A., Bolle P.-A., Bolotin-Fukuhara M., Bossier P., Bou G., Boyer J., Buitrago M.J., Cheret G., Colleaux L., Daignan-Fornier B., del Rey F., Dion C.
      , Domdey H., Duesterhoeft A., Duesterhus S., Entian K.-D., Erfle H., Esteban P.F., Feldmann H., Fernandes L., Fobo G.M., Fritz C., Fukuhara H., Gabel C., Gaillon L., Garcia-Cantalejo J.M., Garcia-Ramirez J.J., Gent M.E., Ghazvini M., Goffeau A., Gonzalez A., Grothues D., Guerreiro P., Hegemann J.H., Hewitt N., Hilger F., Hollenberg C.P., Horaitis O., Indge K.J., Jacquier A., James C.M., Jauniaux J.-C., Jimenez A., Keuchel H., Kirchrath L., Kleine K., Koetter P., Legrain P., Liebl S., Louis E.J., Maia e Silva A., Marck C., Monnier A.-L., Moestl D., Mueller S., Obermaier B., Oliver S.G., Pallier C., Pascolo S., Pfeiffer F., Philippsen P., Planta R.J., Pohl F.M., Pohl T.M., Poehlmann R., Portetelle D., Purnelle B., Puzos V., Ramezani Rad M., Rasmussen S.W., Remacha M.A., Revuelta J.L., Richard G.-F., Rieger M., Rodrigues-Pousada C., Rose M., Rupp T., Santos M.A., Schwager C., Sensen C., Skala J., Soares H., Sor F., Stegemann J., Tettelin H., Thierry A., Tzermia M., Urrestarazu L.A., van Dyck L., van Vliet-Reedijk J.C., Valens M., Vandenbol M., Vilela C., Vissers S., von Wettstein D., Voss H., Wiemann S., Xu G., Zimmermann J., Haasemann M., Becker I., Mewes H.-W.
      Nature 369:371-378(1994) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 204508 / S288c.
    3. Cited for: GENOME REANNOTATION.
      Strain: ATCC 204508 / S288c.
    4. "Cloning and mutational analysis of the gene encoding subunit C of yeast vacuolar H(+)-ATPase."
      Beltran C., Kopecky J., Pan Y.-C.E., Nelson H., Nelson N.
      J. Biol. Chem. 267:774-779(1992) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 257-412.
    5. Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
    6. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
    7. Cited for: ACETYLATION [LARGE SCALE ANALYSIS] AT SER-2, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS], CLEAVAGE OF INITIATOR METHIONINE [LARGE SCALE ANALYSIS].

    Entry informationi

    Entry nameiEF1G2_YEAST
    AccessioniPrimary (citable) accession number: P36008
    Secondary accession number(s): D6VXK6
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: June 1, 1994
    Last sequence update: June 1, 1994
    Last modified: October 1, 2014
    This is version 126 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programFungal Protein Annotation Program

    Miscellaneousi

    Miscellaneous

    Present with 102130 molecules/cell in log phase SD medium.1 Publication

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    2. SIMILARITY comments
      Index of protein domains and families
    3. Yeast
      Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
    4. Yeast chromosome XI
      Yeast (Saccharomyces cerevisiae) chromosome XI: entries and gene names

    External Data

    Dasty 3