Reviewed, UniProtKB/Swiss-Prot P36007 (SRY1_YEAST)

Names and origin

Protein names

Recommended name:
    Threo-3-hydroxyaspartate ammonia-lyase
    EC=4.3.1.16
Alternative name(s):
    L-threo-3-hydroxyaspartate dehydratase

Gene names

Name:	SRY1
Ordered Locus Names:	YKL218C

Organism

Saccharomyces cerevisiae (Baker's yeast) [Complete proteome]

Taxonomic identifier

4932 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces

Protein attributes

Sequence length	326 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Exhibits dehydratase activity specific for L-threo-3-hydroxyaspartate. Ref.3
Catalytic activity	Threo-3-hydroxy-L-aspartate = oxaloacetate + NH3.
Cofactor	Pyridoxal phosphate By similarity. Ref.3
Sequence similarities	Belongs to the serine/threonine dehydratase family.
Biophysicochemical properties	Kinetic parameters: KM=3.9 mM for L-threo-3-hydroxyaspartate Vmax=110 µmol/min/mg enzyme toward L-threo-3-hydroxyaspartate

Ontologies

Keywords
Ligand	Pyridoxal phosphate
Molecular function	Lyase
PTM	Phosphoprotein
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	cellular amino acid derivative catabolic process Ref.3 Inferred from direct assay. Source: SGD cellular amino acid metabolic process Inferred from electronic annotation. Source: InterPro
Molecular function	pyridoxal phosphate binding Inferred from electronic annotation. Source: InterPro threo-3-hydroxyaspartate ammonia-lyase activity Ref.3 Inferred from direct assay. Source: SGD
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 326	326	Threo-3-hydroxyaspartate ammonia-lyase		PRO_0000185589
Amino acid modifications
	Modified residue	53	1	N6-(pyridoxal phosphate)lysine By similarity
	Modified residue	217	1	Phosphoserine Ref.4
	Modified residue	219	1	Phosphoserine Ref.4

Sequences

	Sequence		Length	Mass (Da)
	P36007-1 [UniParc]. Last modified June 1, 1994. Version 1. Checksum: 21CF7FEFC8AB4431	FASTA	326	34,899
	10 20 30 40 50 60 MIVPTYGDVL DASNRIKEYV NKTPVLTSRM LNDRLGAQIY FKGENFQRVG AFKFRGAMNA 70 80 90 100 110 120 VSKLSDEKRS KGVIAFSSGN HAQAIALSAK LLNVPATIVM PEDAPALKVA ATAGYGAHII 130 140 150 160 170 180 RYNRYTEDRE QIGRQLAAEH GFALIPPYDH PDVIAGQGTS AKELLEEVGQ LDALFVPLGG 190 200 210 220 230 240 GGLLSGSALA ARSLSPGCKI FGVEPEAGND GQQSFRSGSI VHINTPKTIA DGAQTQHLGE 250 260 270 280 290 300 YTFAIIRENV DDILTVSDQE LVKCMHFLAE RMKVVVEPTA CLGFAGALLK KEELVGKKVG 310 320 IILSGGNVDM KRYATLISGK EDGPTI

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The complete sequencing of a 24.6 kb segment of yeast chromosome XI identified the known loci URA1, SAC1 and TRP3, and revealed 6 new open reading frames including homologues to the threonine dehydratases, membrane transporters, hydantoinases and the phospholipase A2-activating protein." Tzermia M., Horaitis O., Alexandraki D. Yeast 10:663-679(1994) [PubMed: 7941750] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 204508 / S288c.
[2]	"Complete DNA sequence of yeast chromosome XI." Dujon B., Alexandraki D., Andre B., Ansorge W., Baladron V., Ballesta J.P.G., Banrevi A., Bolle P.-A., Bolotin-Fukuhara M., Bossier P., Bou G., Boyer J., Buitrago M.J., Cheret G., Colleaux L., Daignan-Fornier B., del Rey F., Dion C. , Domdey H., Duesterhoeft A., Duesterhus S., Entian K.-D., Erfle H., Esteban P.F., Feldmann H., Fernandes L., Fobo G.M., Fritz C., Fukuhara H., Gabel C., Gaillon L., Garcia-Cantalejo J.M., Garcia-Ramirez J.J., Gent M.E., Ghazvini M., Goffeau A., Gonzalez A., Grothues D., Guerreiro P., Hegemann J.H., Hewitt N., Hilger F., Hollenberg C.P., Horaitis O., Indge K.J., Jacquier A., James C.M., Jauniaux J.-C., Jimenez A., Keuchel H., Kirchrath L., Kleine K., Koetter P., Legrain P., Liebl S., Louis E.J., Maia e Silva A., Marck C., Monnier A.-L., Moestl D., Mueller S., Obermaier B., Oliver S.G., Pallier C., Pascolo S., Pfeiffer F., Philippsen P., Planta R.J., Pohl F.M., Pohl T.M., Poehlmann R., Portetelle D., Purnelle B., Puzos V., Ramezani Rad M., Rasmussen S.W., Remacha M.A., Revuelta J.L., Richard G.-F., Rieger M., Rodrigues-Pousada C., Rose M., Rupp T., Santos M.A., Schwager C., Sensen C., Skala J., Soares H., Sor F., Stegemann J., Tettelin H., Thierry A., Tzermia M., Urrestarazu L.A., van Dyck L., van Vliet-Reedijk J.C., Valens M., Vandenbol M., Vilela C., Vissers S., von Wettstein D., Voss H., Wiemann S., Xu G., Zimmermann J., Haasemann M., Becker I., Mewes H.-W. Nature 369:371-378(1994) [PubMed: 8196765] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 204508 / S288c.
[3]	"Serine racemase homologue of Saccharomyces cerevisiae has L-threo-3-hydroxyaspartate dehydratase activity." Wada M., Nakamori S., Takagi H. FEMS Microbiol. Lett. 225:189-193(2003) [PubMed: 12951240] [Abstract] Cited for: FUNCTION, COFACTOR, BIOPHYSICOCHEMICAL PROPERTIES.
[4]	"A multidimensional chromatography technology for in-depth phosphoproteome analysis." Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H. Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed: 18407956] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-217 AND SER-219, MASS SPECTROMETRY.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	X75951 Genomic DNA. Translation: CAA53555.1. Z28218 Genomic DNA. Translation: CAA82063.1.
PIR	S38061.
RefSeq	NP_012704.1.
3D structure databases
SMR	P36007. Positions 2-318.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-6523N.
STRING	P36007.
Proteomic databases
PeptideAtlas	P36007.
PRIDE	P36007.
Genome annotation databases
Ensembl	YKL218C; YKL218C; YKL218C; Saccharomyces cerevisiae. [Genome view]
GeneID	853662.
KEGG	sce:YKL218C.
NMPDR	fig|4932.3.peg.3682.
Organism-specific databases
CYGD	YKL218c.
SGD	S000001701. SRY1.
Phylogenomic databases
eggNOG	fuNOG07508.
HOGENOM	HBG714501.
OMA	MTLIPPY.
OrthoDB	EOG9KWM9M.
PhylomeDB	P36007.
Enzyme and pathway databases
BRENDA	4.3.1.16. 250.
Gene expression databases
ArrayExpress	P36007.
Genevestigator	P36007.
GermOnline	YKL218C. Saccharomyces cerevisiae.
Family and domain databases
InterPro	IPR001926. PyrdxlP-dep_enz_bsu. IPR000634. Ser/Thr_deHydtase_PyrdxlP-BS. [Graphical view]
Pfam	PF00291. PALP. 1 hit. [Graphical view]
PROSITE	PS00165. DEHYDRATASE_SER_THR. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	974590.

Entry information

Entry name

SRY1_YEAST

Accession

Primary (citable) accession number: P36007

Entry history

Integrated into UniProtKB/Swiss-Prot:	June 1, 1994
Last sequence update:	June 1, 1994
Last modified:	January 19, 2010
This is version 74 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

FPAP (Fungal Proteome Annotation Project)