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Protein

Threo-3-hydroxyaspartate ammonia-lyase

Gene

SRY1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Exhibits dehydratase activity specific for L-threo-3-hydroxyaspartate.1 Publication

Catalytic activityi

Threo-3-hydroxy-L-aspartate = oxaloacetate + NH3.

Cofactori

Kineticsi

  1. KM=3.9 mM for L-threo-3-hydroxyaspartate1 Publication
  1. Vmax=110 µmol/min/mg enzyme toward L-threo-3-hydroxyaspartate1 Publication

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Lyase

Keywords - Ligandi

Pyridoxal phosphate

Enzyme and pathway databases

BioCyciRETL1328306-WGS:GSTH-3605-MONOMER.
YEAST:YKL218C-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Threo-3-hydroxyaspartate ammonia-lyase (EC:4.3.1.16)
Alternative name(s):
L-threo-3-hydroxyaspartate dehydratase
Gene namesi
Name:SRY1
Ordered Locus Names:YKL218C
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome XI

Organism-specific databases

EuPathDBiFungiDB:YKL218C.
SGDiS000001701. SRY1.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 326326Threo-3-hydroxyaspartate ammonia-lyasePRO_0000185589Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei53 – 531N6-(pyridoxal phosphate)lysineBy similarity

Proteomic databases

MaxQBiP36007.

Interactioni

Protein-protein interaction databases

BioGridi33947. 95 interactions.
DIPiDIP-6523N.
IntActiP36007. 2 interactions.

Structurei

3D structure databases

ProteinModelPortaliP36007.
SMRiP36007. Positions 1-318.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

GeneTreeiENSGT00550000075026.
HOGENOMiHOG000046974.
InParanoidiP36007.
OMAiMRDAMAY.
OrthoDBiEOG092C3P3F.

Family and domain databases

InterProiIPR000634. Ser/Thr_deHydtase_PyrdxlP-BS.
IPR001926. TrpB-like_PLP-dep.
[Graphical view]
PfamiPF00291. PALP. 1 hit.
[Graphical view]
SUPFAMiSSF53686. SSF53686. 1 hit.
PROSITEiPS00165. DEHYDRATASE_SER_THR. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P36007-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MIVPTYGDVL DASNRIKEYV NKTPVLTSRM LNDRLGAQIY FKGENFQRVG
60 70 80 90 100
AFKFRGAMNA VSKLSDEKRS KGVIAFSSGN HAQAIALSAK LLNVPATIVM
110 120 130 140 150
PEDAPALKVA ATAGYGAHII RYNRYTEDRE QIGRQLAAEH GFALIPPYDH
160 170 180 190 200
PDVIAGQGTS AKELLEEVGQ LDALFVPLGG GGLLSGSALA ARSLSPGCKI
210 220 230 240 250
FGVEPEAGND GQQSFRSGSI VHINTPKTIA DGAQTQHLGE YTFAIIRENV
260 270 280 290 300
DDILTVSDQE LVKCMHFLAE RMKVVVEPTA CLGFAGALLK KEELVGKKVG
310 320
IILSGGNVDM KRYATLISGK EDGPTI
Length:326
Mass (Da):34,899
Last modified:June 1, 1994 - v1
Checksum:i21CF7FEFC8AB4431
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X75951 Genomic DNA. Translation: CAA53555.1.
Z28218 Genomic DNA. Translation: CAA82063.1.
BK006944 Genomic DNA. Translation: DAA08951.1.
PIRiS38061.
RefSeqiNP_012704.1. NM_001179783.1.

Genome annotation databases

EnsemblFungiiYKL218C; YKL218C; YKL218C.
GeneIDi853662.
KEGGisce:YKL218C.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X75951 Genomic DNA. Translation: CAA53555.1.
Z28218 Genomic DNA. Translation: CAA82063.1.
BK006944 Genomic DNA. Translation: DAA08951.1.
PIRiS38061.
RefSeqiNP_012704.1. NM_001179783.1.

3D structure databases

ProteinModelPortaliP36007.
SMRiP36007. Positions 1-318.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi33947. 95 interactions.
DIPiDIP-6523N.
IntActiP36007. 2 interactions.

Proteomic databases

MaxQBiP36007.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYKL218C; YKL218C; YKL218C.
GeneIDi853662.
KEGGisce:YKL218C.

Organism-specific databases

EuPathDBiFungiDB:YKL218C.
SGDiS000001701. SRY1.

Phylogenomic databases

GeneTreeiENSGT00550000075026.
HOGENOMiHOG000046974.
InParanoidiP36007.
OMAiMRDAMAY.
OrthoDBiEOG092C3P3F.

Enzyme and pathway databases

BioCyciRETL1328306-WGS:GSTH-3605-MONOMER.
YEAST:YKL218C-MONOMER.

Miscellaneous databases

PROiP36007.

Family and domain databases

InterProiIPR000634. Ser/Thr_deHydtase_PyrdxlP-BS.
IPR001926. TrpB-like_PLP-dep.
[Graphical view]
PfamiPF00291. PALP. 1 hit.
[Graphical view]
SUPFAMiSSF53686. SSF53686. 1 hit.
PROSITEiPS00165. DEHYDRATASE_SER_THR. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiSRY1_YEAST
AccessioniPrimary (citable) accession number: P36007
Secondary accession number(s): D6VWY5
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 1, 1994
Last sequence update: June 1, 1994
Last modified: September 7, 2016
This is version 129 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  3. Yeast chromosome XI
    Yeast (Saccharomyces cerevisiae) chromosome XI: entries and gene names

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.