P36005 (KDX1_YEAST) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Serine/threonine-protein kinase KDX1
EC=2.7.11.1

Alternative name(s):
Kinase dead X-talker protein 1
MPK1-like protein kinase 1

Gene names

Name:	KDX1
Synonyms:	MLP1
Ordered Locus Names:	YKL161C
ORF Names:	YKL615

Organism

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)

Taxonomic identifier

559292 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces

Protein attributes

Sequence length	433 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Serine/threonine-protein kinase involved in the SLT2 mitogen-activated (MAP) kinase signaling pathway that regulates cell wall integrity. May also be involved in the mating pheromone and the CWI MAPK pathways. Ref.4 Ref.5 Ref.7
Catalytic activity	ATP + a protein = ADP + a phosphoprotein.
Subunit structure	Interacts with RLM1. Ref.4
Induction	Accumulates in large amounts when cell wall integrity is compromised. Ref.6
Sequence similarities	Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. Contains 1 protein kinase domain.

Ontologies

Keywords
Ligand	ATP-binding Nucleotide-binding
Molecular function	Kinase Serine/threonine-protein kinase Transferase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW protein serine/threonine kinase activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 433	433	Serine/threonine-protein kinase KDX1		PRO_0000086150
Regions
	Domain	23 – 318	296	Protein kinase
	Nucleotide binding	29 – 37	9	ATP By similarity
Sites
	Active site	153	1	Proton acceptor By similarity
	Binding site	55	1	ATP By similarity

Sequences

	Sequence		Length	Mass (Da)
	P36005 [UniParc]. Last modified June 1, 1994. Version 1. Checksum: BC27B76B7DB95822	FASTA	433	49,633
	10 20 30 40 50 60 MATDTERCIF RAFGQDFILN KHFHLTGKIG RGSHSLICSS TYTESNEETH VAIRKIPNAF 70 80 90 100 110 120 GNKLSCKRTL RELKLLRHLR GHPNIVWLFD TDIVFYPNGA LNGVYLYEEL MECDLSQIIR 130 140 150 160 170 180 SEQRLEDAHF QSFIYQILCA LKYIHSANVL HCDLKPKNLL VNSDCQLKIC NFGLSCSYSE 190 200 210 220 230 240 NHKVNDGFIK GYITSIWYKA PEILLNYQEC TKAVDIWSTG CILAELLGRK PMFEGKDYVD 250 260 270 280 290 300 HLNHILQILG TPPEETLQEI ASQKVYNYIF QFGNIPGRSF ESILPGANPE ALELLKKMLE 310 320 330 340 350 360 FDPKKRITVE DALEHPYLSM WHDIDEEFSC QKTFRFEFEH IESMAELGNE VIKEVFDFRK 370 380 390 400 410 420 VVRKHPISGD SPSSSLSLED AIPQEVVQVH PSRKVLPSYS PEFSYVSQLP SLTTTQPYQN 430 LMGISSNSFQ GVN

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"DNA sequencing of a 36.2 kb fragment located between the FAS1 and LAP loci of chromosome XI of Saccharomyces cerevisiae." Vandenbol M., Bolle P.-A., Dion C., Portetelle D., Hilger F. Yeast 10:S35-S40(1994) [PubMed: 8091859] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 204508 / S288c.
[2]	"Complete DNA sequence of yeast chromosome XI." Dujon B., Alexandraki D., Andre B., Ansorge W., Baladron V., Ballesta J.P.G., Banrevi A., Bolle P.-A., Bolotin-Fukuhara M., Bossier P., Bou G., Boyer J., Buitrago M.J., Cheret G., Colleaux L., Daignan-Fornier B., del Rey F., Dion C. , Domdey H., Duesterhoeft A., Duesterhus S., Entian K.-D., Erfle H., Esteban P.F., Feldmann H., Fernandes L., Fobo G.M., Fritz C., Fukuhara H., Gabel C., Gaillon L., Garcia-Cantalejo J.M., Garcia-Ramirez J.J., Gent M.E., Ghazvini M., Goffeau A., Gonzalez A., Grothues D., Guerreiro P., Hegemann J.H., Hewitt N., Hilger F., Hollenberg C.P., Horaitis O., Indge K.J., Jacquier A., James C.M., Jauniaux J.-C., Jimenez A., Keuchel H., Kirchrath L., Kleine K., Koetter P., Legrain P., Liebl S., Louis E.J., Maia e Silva A., Marck C., Monnier A.-L., Moestl D., Mueller S., Obermaier B., Oliver S.G., Pallier C., Pascolo S., Pfeiffer F., Philippsen P., Planta R.J., Pohl F.M., Pohl T.M., Poehlmann R., Portetelle D., Purnelle B., Puzos V., Ramezani Rad M., Rasmussen S.W., Remacha M.A., Revuelta J.L., Richard G.-F., Rieger M., Rodrigues-Pousada C., Rose M., Rupp T., Santos M.A., Schwager C., Sensen C., Skala J., Soares H., Sor F., Stegemann J., Tettelin H., Thierry A., Tzermia M., Urrestarazu L.A., van Dyck L., van Vliet-Reedijk J.C., Valens M., Vandenbol M., Vilela C., Vissers S., von Wettstein D., Voss H., Wiemann S., Xu G., Zimmermann J., Haasemann M., Becker I., Mewes H.-W. Nature 369:371-378(1994) [PubMed: 8196765] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 204508 / S288c.
[3]	Saccharomyces Genome Database Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: ATCC 204508 / S288c.
[4]	"Characterization of a serum response factor-like protein in Saccharomyces cerevisiae, Rlm1, which has transcriptional activity regulated by the Mpk1 (Slt2) mitogen-activated protein kinase pathway." Watanabe Y., Takaesu G., Hagiwara M., Irie K., Matsumoto K. Mol. Cell. Biol. 17:2615-2623(1997) [PubMed: 9111331] [Abstract] Cited for: FUNCTION, INTERACTION WITH RLM1.
[5]	"Analysis of yeast protein kinases using protein chips." Zhu H., Klemic J.F., Chang S., Bertone P., Casamayor A., Klemic K.G., Smith D., Gerstein M., Reed M.A., Snyder M. Nat. Genet. 26:283-289(2000) [PubMed: 11062466] [Abstract] Cited for: FUNCTION.
[6]	"A yeast strain biosensor to detect cell wall-perturbing agents." Rodriguez-Pena J.M., Diez-Muniz S., Nombela C., Arroyo J. J. Biotechnol. 133:311-317(2008) [PubMed: 18055054] [Abstract] Cited for: INDUCTION.
[7]	"A global protein kinase and phosphatase interaction network in yeast." Breitkreutz A., Choi H., Sharom J.R., Boucher L., Neduva V., Larsen B., Lin Z.Y., Breitkreutz B.J., Stark C., Liu G., Ahn J., Dewar-Darch D., Reguly T., Tang X., Almeida R., Qin Z.S., Pawson T., Gingras A.C., Nesvizhskii A.I., Tyers M. Science 328:1043-1046(2010) [PubMed: 20489023] [Abstract] Cited for: FUNCTION.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	Z26877 Genomic DNA. Translation: CAA81493.1. Z28161 Genomic DNA. Translation: CAA82003.1. BK006944 Genomic DNA. Translation: DAA09004.1.
PIR	S37790.
RefSeq	NP_012761.1. NM_001179727.1.
3D structure databases
ProteinModelPortal	P36005.
SMR	P36005. Positions 15-338.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-6316N.
IntAct	P36005. 22 interactions.
MINT	MINT-698763.
STRING	P36005.
Proteomic databases
PeptideAtlas	P36005.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblFungi	YKL161C; YKL161C; YKL161C.
GeneID	853696.
KEGG	sce:YKL161C.
NMPDR	fig|4932.3.peg.3740.
Organism-specific databases
CYGD	YKL161c.
SGD	S000001644. KDX1.
Phylogenomic databases
eggNOG	fuNOG04682.
GeneTree	EFGT00050000000591.
HOGENOM	HBG755340.
OMA	TERCIFR.
OrthoDB	EOG4S7NZG.
Gene expression databases
ArrayExpress	P36005.
Genevestigator	P36005.
GermOnline	YKL161C. Saccharomyces cerevisiae.
Family and domain databases
InterPro	IPR011009. Kinase-like_dom. IPR000719. Prot_kinase_cat_dom. IPR017441. Protein_kinase_ATP_BS. IPR017442. Se/Thr_kinase-like_dom. IPR008271. Ser/Thr_kinase_AS. IPR002290. Ser/Thr_kinase_dom. [Graphical view]
KO	K08293.
Pfam	PF00069. Pkinase. 1 hit. [Graphical view]
SMART	SM00220. S_TKc. 1 hit. [Graphical view]
SUPFAM	SSF56112. Kinase_like. 1 hit.
PROSITE	PS00107. PROTEIN_KINASE_ATP. 1 hit. PS50011. PROTEIN_KINASE_DOM. 1 hit. PS00108. PROTEIN_KINASE_ST. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	974678.

Entry information

Entry name

KDX1_YEAST

Accession

Primary (citable) accession number: P36005
Secondary accession number(s): D6VX38

Entry history

Integrated into UniProtKB/Swiss-Prot:	June 1, 1994
Last sequence update:	June 1, 1994
Last modified:	December 14, 2011
This is version 101 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program