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P36004

- KKQ8_YEAST

UniProt

P36004 - KKQ8_YEAST

Protein

Probable serine/threonine-protein kinase KKQ8

Gene

KKQ8

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 127 (01 Oct 2014)
      Sequence version 2 (20 May 2008)
      Previous versions | rss
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    Functioni

    Catalytic activityi

    ATP + a protein = ADP + a phosphoprotein.

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei455 – 4551ATPPROSITE-ProRule annotation
    Active sitei563 – 5631Proton acceptorPROSITE-ProRule annotation

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi418 – 4269ATPPROSITE-ProRule annotation

    GO - Molecular functioni

    1. ATP binding Source: UniProtKB-KW
    2. protein kinase activity Source: SGD
    3. protein serine/threonine kinase activity Source: UniProtKB-KW

    Keywords - Molecular functioni

    Kinase, Serine/threonine-protein kinase, Transferase

    Keywords - Ligandi

    ATP-binding, Nucleotide-binding

    Enzyme and pathway databases

    BioCyciYEAST:G3O-31936-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Probable serine/threonine-protein kinase KKQ8 (EC:2.7.11.1)
    Gene namesi
    Name:KKQ8
    Ordered Locus Names:YKL168C
    ORF Names:YKL632
    OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
    Taxonomic identifieri559292 [NCBI]
    Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
    ProteomesiUP000002311: Chromosome XI

    Organism-specific databases

    SGDiS000001651. KKQ8.

    Subcellular locationi

    Cytoplasm 1 Publication

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cytoplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 724724Probable serine/threonine-protein kinase KKQ8PRO_0000086151Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei19 – 191Phosphoserine1 Publication
    Modified residuei232 – 2321Phosphoserine3 Publications
    Modified residuei238 – 2381Phosphoserine1 Publication
    Modified residuei241 – 2411Phosphoserine1 Publication

    Keywords - PTMi

    Phosphoprotein

    Proteomic databases

    MaxQBiP36004.
    PaxDbiP36004.

    Expressioni

    Gene expression databases

    GenevestigatoriP36004.

    Interactioni

    Protein-protein interaction databases

    BioGridi33969. 94 interactions.
    DIPiDIP-4838N.
    IntActiP36004. 6 interactions.
    MINTiMINT-473137.
    STRINGi4932.YKL168C.

    Structurei

    3D structure databases

    ProteinModelPortaliP36004.
    SMRiP36004. Positions 380-724.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini412 – 712301Protein kinasePROSITE-ProRule annotationAdd
    BLAST

    Sequence similaritiesi

    Contains 1 protein kinase domain.PROSITE-ProRule annotation

    Phylogenomic databases

    eggNOGiCOG0515.
    GeneTreeiENSGT00550000075309.
    HOGENOMiHOG000112846.
    KOiK08286.
    OrthoDBiEOG7008J9.

    Family and domain databases

    InterProiIPR011009. Kinase-like_dom.
    IPR000719. Prot_kinase_dom.
    IPR008271. Ser/Thr_kinase_AS.
    [Graphical view]
    PfamiPF00069. Pkinase. 1 hit.
    [Graphical view]
    SUPFAMiSSF56112. SSF56112. 1 hit.
    PROSITEiPS50011. PROTEIN_KINASE_DOM. 1 hit.
    PS00108. PROTEIN_KINASE_ST. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P36004-1 [UniParc]FASTAAdd to Basket

    « Hide

    MVMQEEKKRQ QPVTRRVRSF SESFKNLFRP PRSRDSSPIN VTRIPYRSSS    50
    TSPKRSSEPP RRSTVSAQIL DPKNSPIRQR SYTLKCCTPG LSHPFRQTGS 100
    GASNSPTRHR SISGEEQEIV NSLPEYKRSA SHTFHGIRRP RSRSSSVSSC 150
    DSSNGTTSSS DSQWAMDSLL DDSDNDLTPY RGSNKDILKS KDRAPYNYID 200
    DYNKKALRRA TSYPNPLPSK QFYNERLYTR RSHPDEESLE SLPRFAGADV 250
    QCIIEQNGFK VYEDGSHEHN IKLSGVIAKL EKGNSLPAHR QGSLSRPRLG 300
    ITLSGLFKHH KNECDIENAL SLLPNVEKSQ TNHEKRTGQS PNDSNRSSPT 350
    QGREDYLKIV NPDASLGSDE LKLINSLSSR IHKSLQNYLQ EKNLKPAECI 400
    GEQAPTFQDN YGHPVGLVGA GAYGEVKLCA RLRNEKDSPP FETYHDSKYI 450
    YYAVKELKPK PDSDLEKFCT KITSEFIIGH SLSHYHKNGK KPAPNILNVF 500
    DILEDSSSFI EVMEFCPAGD LYGMLVGKSK LKGRLHPLEA DCFMKQLLHG 550
    VKFMHDHGIA HCDLKPENIL FYPHGLLKIC DFGTSSVFQT AWERRVHAQK 600
    GIIGSEPYVA PEEFVDGEYY DPRLIDCWSC GVVYITMILG HYLWKVASRE 650
    KDMSYDEFYK EMQRKNQFRV FEELKHVNSE LATNRKIALY RIFQWEPRKR 700
    ISVGKLLDMQ WMKSTNCCLI YDST 724
    Length:724
    Mass (Da):82,590
    Last modified:May 20, 2008 - v2
    Checksum:i7EAC570CE6E5154F
    GO

    Sequence cautioni

    The sequence CAA81519.1 differs from that shown. Reason: Erroneous initiation.
    The sequence CAA82010.1 differs from that shown. Reason: Erroneous initiation.

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    Z26878 Genomic DNA. Translation: CAA81519.1. Different initiation.
    Z28168 Genomic DNA. Translation: CAA82010.1. Different initiation.
    BK006944 Genomic DNA. Translation: DAA08997.1.
    PIRiS37998.
    RefSeqiNP_012753.2. NM_001179734.1.

    Genome annotation databases

    EnsemblFungiiYKL168C; YKL168C; YKL168C.
    GeneIDi853686.
    KEGGisce:YKL168C.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    Z26878 Genomic DNA. Translation: CAA81519.1 . Different initiation.
    Z28168 Genomic DNA. Translation: CAA82010.1 . Different initiation.
    BK006944 Genomic DNA. Translation: DAA08997.1 .
    PIRi S37998.
    RefSeqi NP_012753.2. NM_001179734.1.

    3D structure databases

    ProteinModelPortali P36004.
    SMRi P36004. Positions 380-724.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 33969. 94 interactions.
    DIPi DIP-4838N.
    IntActi P36004. 6 interactions.
    MINTi MINT-473137.
    STRINGi 4932.YKL168C.

    Proteomic databases

    MaxQBi P36004.
    PaxDbi P36004.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblFungii YKL168C ; YKL168C ; YKL168C .
    GeneIDi 853686.
    KEGGi sce:YKL168C.

    Organism-specific databases

    SGDi S000001651. KKQ8.

    Phylogenomic databases

    eggNOGi COG0515.
    GeneTreei ENSGT00550000075309.
    HOGENOMi HOG000112846.
    KOi K08286.
    OrthoDBi EOG7008J9.

    Enzyme and pathway databases

    BioCyci YEAST:G3O-31936-MONOMER.

    Miscellaneous databases

    NextBioi 974655.

    Gene expression databases

    Genevestigatori P36004.

    Family and domain databases

    InterProi IPR011009. Kinase-like_dom.
    IPR000719. Prot_kinase_dom.
    IPR008271. Ser/Thr_kinase_AS.
    [Graphical view ]
    Pfami PF00069. Pkinase. 1 hit.
    [Graphical view ]
    SUPFAMi SSF56112. SSF56112. 1 hit.
    PROSITEi PS50011. PROTEIN_KINASE_DOM. 1 hit.
    PS00108. PROTEIN_KINASE_ST. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Sequencing and analysis of a 20.5 kb DNA segment located on the left arm of yeast chromosome XI."
      Vandenbol M., Bolle P.-A., Dion C., Portetelle D., Hilger F.
      Yeast 10:S25-S33(1994) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: ATCC 204508 / S288c.
    2. "Complete DNA sequence of yeast chromosome XI."
      Dujon B., Alexandraki D., Andre B., Ansorge W., Baladron V., Ballesta J.P.G., Banrevi A., Bolle P.-A., Bolotin-Fukuhara M., Bossier P., Bou G., Boyer J., Buitrago M.J., Cheret G., Colleaux L., Daignan-Fornier B., del Rey F., Dion C.
      , Domdey H., Duesterhoeft A., Duesterhus S., Entian K.-D., Erfle H., Esteban P.F., Feldmann H., Fernandes L., Fobo G.M., Fritz C., Fukuhara H., Gabel C., Gaillon L., Garcia-Cantalejo J.M., Garcia-Ramirez J.J., Gent M.E., Ghazvini M., Goffeau A., Gonzalez A., Grothues D., Guerreiro P., Hegemann J.H., Hewitt N., Hilger F., Hollenberg C.P., Horaitis O., Indge K.J., Jacquier A., James C.M., Jauniaux J.-C., Jimenez A., Keuchel H., Kirchrath L., Kleine K., Koetter P., Legrain P., Liebl S., Louis E.J., Maia e Silva A., Marck C., Monnier A.-L., Moestl D., Mueller S., Obermaier B., Oliver S.G., Pallier C., Pascolo S., Pfeiffer F., Philippsen P., Planta R.J., Pohl F.M., Pohl T.M., Poehlmann R., Portetelle D., Purnelle B., Puzos V., Ramezani Rad M., Rasmussen S.W., Remacha M.A., Revuelta J.L., Richard G.-F., Rieger M., Rodrigues-Pousada C., Rose M., Rupp T., Santos M.A., Schwager C., Sensen C., Skala J., Soares H., Sor F., Stegemann J., Tettelin H., Thierry A., Tzermia M., Urrestarazu L.A., van Dyck L., van Vliet-Reedijk J.C., Valens M., Vandenbol M., Vilela C., Vissers S., von Wettstein D., Voss H., Wiemann S., Xu G., Zimmermann J., Haasemann M., Becker I., Mewes H.-W.
      Nature 369:371-378(1994) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 204508 / S288c.
    3. Cited for: GENOME REANNOTATION.
      Strain: ATCC 204508 / S288c.
    4. "Sequencing and comparison of yeast species to identify genes and regulatory elements."
      Kellis M., Patterson N., Endrizzi M., Birren B.W., Lander E.S.
      Nature 423:241-254(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: IDENTIFICATION OF PROBABLE INITIATION SITE.
    5. Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
    6. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
    7. "Finding functional features in Saccharomyces genomes by phylogenetic footprinting."
      Cliften P.F., Sudarsanam P., Desikan A., Fulton L., Fulton B., Majors J., Waterston R., Cohen B.A., Johnston M.
      Science 301:71-76(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: IDENTIFICATION OF PROBABLE INITIATION SITE.
    8. "Large-scale phosphorylation analysis of alpha-factor-arrested Saccharomyces cerevisiae."
      Li X., Gerber S.A., Rudner A.D., Beausoleil S.A., Haas W., Villen J., Elias J.E., Gygi S.P.
      J. Proteome Res. 6:1190-1197(2007) [PubMed] [Europe PMC] [Abstract]
      Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-232; SER-238 AND SER-241, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
      Strain: ADR376.
    9. "Analysis of phosphorylation sites on proteins from Saccharomyces cerevisiae by electron transfer dissociation (ETD) mass spectrometry."
      Chi A., Huttenhower C., Geer L.Y., Coon J.J., Syka J.E.P., Bai D.L., Shabanowitz J., Burke D.J., Troyanskaya O.G., Hunt D.F.
      Proc. Natl. Acad. Sci. U.S.A. 104:2193-2198(2007) [PubMed] [Europe PMC] [Abstract]
      Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-19, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    10. "A multidimensional chromatography technology for in-depth phosphoproteome analysis."
      Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.
      Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract]
      Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-232, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    11. "Global analysis of Cdk1 substrate phosphorylation sites provides insights into evolution."
      Holt L.J., Tuch B.B., Villen J., Johnson A.D., Gygi S.P., Morgan D.O.
      Science 325:1682-1686(2009) [PubMed] [Europe PMC] [Abstract]
      Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-232, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

    Entry informationi

    Entry nameiKKQ8_YEAST
    AccessioniPrimary (citable) accession number: P36004
    Secondary accession number(s): D6VX31
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: June 1, 1994
    Last sequence update: May 20, 2008
    Last modified: October 1, 2014
    This is version 127 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programFungal Protein Annotation Program

    Miscellaneousi

    Miscellaneous

    Present with 981 molecules/cell in log phase SD medium.1 Publication

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families
    2. Yeast
      Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
    3. Yeast chromosome XI
      Yeast (Saccharomyces cerevisiae) chromosome XI: entries and gene names

    External Data

    Dasty 3