P36004 (KKQ8_YEAST) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Probable serine/threonine-protein kinase KKQ8
EC=2.7.11.1

Gene names

Name:	KKQ8
Ordered Locus Names:	YKL168C
ORF Names:	YKL632

Organism

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]

Taxonomic identifier

559292 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Fungi › Dikarya › Ascomycota › saccharomyceta › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces › Saccharomyces cerevisiae

Protein attributes

Sequence length	724 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Catalytic activity	ATP + a protein = ADP + a phosphoprotein.
Subcellular location	Cytoplasm Ref.5.
Miscellaneous	Present with 981 molecules/cell in log phase SD medium.
Sequence similarities	Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. NPR/HAL subfamily. HAL5 sub-subfamily. Contains 1 protein kinase domain.
Sequence caution	The sequence CAA81519.1 differs from that shown. Reason: Erroneous initiation. The sequence CAA82010.1 differs from that shown. Reason: Erroneous initiation.

Ontologies

Keywords
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Kinase Serine/threonine-protein kinase Transferase
PTM	Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Cellular_component	cytoplasm Inferred from direct assay Ref.5. Source: SGD
Molecular_function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW protein kinase activity Inferred from sequence or structural similarity PubMed 9020587. Source: SGD protein serine/threonine kinase activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 724	724	Probable serine/threonine-protein kinase KKQ8		PRO_0000086151
Regions
	Domain	412 – 712	301	Protein kinase
	Nucleotide binding	418 – 426	9	ATP By similarity
Sites
	Active site	563	1	Proton acceptor By similarity
	Binding site	455	1	ATP By similarity
Amino acid modifications
	Modified residue	19	1	Phosphoserine Ref.10 Ref.11
	Modified residue	21	1	Phosphoserine Ref.8 Ref.12
	Modified residue	37	1	Phosphoserine Ref.12
	Modified residue	75	1	Phosphoserine Ref.12
	Modified residue	111	1	Phosphoserine Ref.12
	Modified residue	113	1	Phosphoserine Ref.12
	Modified residue	212	1	Phosphoserine Ref.12
	Modified residue	232	1	Phosphoserine Ref.9 Ref.12
	Modified residue	238	1	Phosphoserine Ref.9 Ref.11 Ref.12
	Modified residue	241	1	Phosphoserine Ref.9 Ref.11 Ref.12

Sequences

	Sequence		Length	Mass (Da)
	P36004 [UniParc]. Last modified May 20, 2008. Version 2. Checksum: 7EAC570CE6E5154F	FASTA	724	82,590
	10 20 30 40 50 60 MVMQEEKKRQ QPVTRRVRSF SESFKNLFRP PRSRDSSPIN VTRIPYRSSS TSPKRSSEPP 70 80 90 100 110 120 RRSTVSAQIL DPKNSPIRQR SYTLKCCTPG LSHPFRQTGS GASNSPTRHR SISGEEQEIV 130 140 150 160 170 180 NSLPEYKRSA SHTFHGIRRP RSRSSSVSSC DSSNGTTSSS DSQWAMDSLL DDSDNDLTPY 190 200 210 220 230 240 RGSNKDILKS KDRAPYNYID DYNKKALRRA TSYPNPLPSK QFYNERLYTR RSHPDEESLE 250 260 270 280 290 300 SLPRFAGADV QCIIEQNGFK VYEDGSHEHN IKLSGVIAKL EKGNSLPAHR QGSLSRPRLG 310 320 330 340 350 360 ITLSGLFKHH KNECDIENAL SLLPNVEKSQ TNHEKRTGQS PNDSNRSSPT QGREDYLKIV 370 380 390 400 410 420 NPDASLGSDE LKLINSLSSR IHKSLQNYLQ EKNLKPAECI GEQAPTFQDN YGHPVGLVGA 430 440 450 460 470 480 GAYGEVKLCA RLRNEKDSPP FETYHDSKYI YYAVKELKPK PDSDLEKFCT KITSEFIIGH 490 500 510 520 530 540 SLSHYHKNGK KPAPNILNVF DILEDSSSFI EVMEFCPAGD LYGMLVGKSK LKGRLHPLEA 550 560 570 580 590 600 DCFMKQLLHG VKFMHDHGIA HCDLKPENIL FYPHGLLKIC DFGTSSVFQT AWERRVHAQK 610 620 630 640 650 660 GIIGSEPYVA PEEFVDGEYY DPRLIDCWSC GVVYITMILG HYLWKVASRE KDMSYDEFYK 670 680 690 700 710 720 EMQRKNQFRV FEELKHVNSE LATNRKIALY RIFQWEPRKR ISVGKLLDMQ WMKSTNCCLI YDST

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Sequencing and analysis of a 20.5 kb DNA segment located on the left arm of yeast chromosome XI." Vandenbol M., Bolle P.-A., Dion C., Portetelle D., Hilger F. Yeast 10:S25-S33(1994) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 204508 / S288c.
[2]	"Complete DNA sequence of yeast chromosome XI." Dujon B., Alexandraki D., Andre B., Ansorge W., Baladron V., Ballesta J.P.G., Banrevi A., Bolle P.-A., Bolotin-Fukuhara M., Bossier P., Bou G., Boyer J., Buitrago M.J., Cheret G., Colleaux L., Daignan-Fornier B., del Rey F., Dion C. , Domdey H., Duesterhoeft A., Duesterhus S., Entian K.-D., Erfle H., Esteban P.F., Feldmann H., Fernandes L., Fobo G.M., Fritz C., Fukuhara H., Gabel C., Gaillon L., Garcia-Cantalejo J.M., Garcia-Ramirez J.J., Gent M.E., Ghazvini M., Goffeau A., Gonzalez A., Grothues D., Guerreiro P., Hegemann J.H., Hewitt N., Hilger F., Hollenberg C.P., Horaitis O., Indge K.J., Jacquier A., James C.M., Jauniaux J.-C., Jimenez A., Keuchel H., Kirchrath L., Kleine K., Koetter P., Legrain P., Liebl S., Louis E.J., Maia e Silva A., Marck C., Monnier A.-L., Moestl D., Mueller S., Obermaier B., Oliver S.G., Pallier C., Pascolo S., Pfeiffer F., Philippsen P., Planta R.J., Pohl F.M., Pohl T.M., Poehlmann R., Portetelle D., Purnelle B., Puzos V., Ramezani Rad M., Rasmussen S.W., Remacha M.A., Revuelta J.L., Richard G.-F., Rieger M., Rodrigues-Pousada C., Rose M., Rupp T., Santos M.A., Schwager C., Sensen C., Skala J., Soares H., Sor F., Stegemann J., Tettelin H., Thierry A., Tzermia M., Urrestarazu L.A., van Dyck L., van Vliet-Reedijk J.C., Valens M., Vandenbol M., Vilela C., Vissers S., von Wettstein D., Voss H., Wiemann S., Xu G., Zimmermann J., Haasemann M., Becker I., Mewes H.-W. Nature 369:371-378(1994) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 204508 / S288c.
[3]	Saccharomyces Genome Database Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: ATCC 204508 / S288c.
[4]	"Sequencing and comparison of yeast species to identify genes and regulatory elements." Kellis M., Patterson N., Endrizzi M., Birren B.W., Lander E.S. Nature 423:241-254(2003) [PubMed] [Europe PMC] [Abstract] Cited for: IDENTIFICATION OF PROBABLE INITIATION SITE.
[5]	"Global analysis of protein localization in budding yeast." Huh W.-K., Falvo J.V., Gerke L.C., Carroll A.S., Howson R.W., Weissman J.S., O'Shea E.K. Nature 425:686-691(2003) [PubMed] [Europe PMC] [Abstract] Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
[6]	"Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed] [Europe PMC] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[7]	"Finding functional features in Saccharomyces genomes by phylogenetic footprinting." Cliften P.F., Sudarsanam P., Desikan A., Fulton L., Fulton B., Majors J., Waterston R., Cohen B.A., Johnston M. Science 301:71-76(2003) [PubMed] [Europe PMC] [Abstract] Cited for: IDENTIFICATION OF PROBABLE INITIATION SITE.
[8]	"Quantitative phosphoproteomics applied to the yeast pheromone signaling pathway." Gruhler A., Olsen J.V., Mohammed S., Mortensen P., Faergeman N.J., Mann M., Jensen O.N. Mol. Cell. Proteomics 4:310-327(2005) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-21, MASS SPECTROMETRY. Strain: YAL6B.
[9]	"Large-scale phosphorylation analysis of alpha-factor-arrested Saccharomyces cerevisiae." Li X., Gerber S.A., Rudner A.D., Beausoleil S.A., Haas W., Villen J., Elias J.E., Gygi S.P. J. Proteome Res. 6:1190-1197(2007) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-232; SER-238 AND SER-241, MASS SPECTROMETRY. Strain: ADR376.
[10]	"Analysis of phosphorylation sites on proteins from Saccharomyces cerevisiae by electron transfer dissociation (ETD) mass spectrometry." Chi A., Huttenhower C., Geer L.Y., Coon J.J., Syka J.E.P., Bai D.L., Shabanowitz J., Burke D.J., Troyanskaya O.G., Hunt D.F. Proc. Natl. Acad. Sci. U.S.A. 104:2193-2198(2007) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-19, MASS SPECTROMETRY.
[11]	"Proteome-wide identification of in vivo targets of DNA damage checkpoint kinases." Smolka M.B., Albuquerque C.P., Chen S.H., Zhou H. Proc. Natl. Acad. Sci. U.S.A. 104:10364-10369(2007) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-19; SER-238 AND SER-241, MASS SPECTROMETRY.
[12]	"A multidimensional chromatography technology for in-depth phosphoproteome analysis." Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H. Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-21; SER-37; SER-75; SER-111; SER-113; SER-212; SER-232; SER-238 AND SER-241, MASS SPECTROMETRY.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	Z26878 Genomic DNA. Translation: CAA81519.1. Different initiation. Z28168 Genomic DNA. Translation: CAA82010.1. Different initiation. BK006944 Genomic DNA. Translation: DAA08997.1.
PIR	S37998.
RefSeq	NP_012753.2. NM_001179734.1.
3D structure databases
ProteinModelPortal	P36004.
SMR	P36004. Positions 380-724.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-4838N.
IntAct	P36004. 6 interactions.
MINT	MINT-473137.
STRING	4932.YKL168C.
Proteomic databases
PaxDb	P36004.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblFungi	YKL168C; YKL168C; YKL168C.
GeneID	853686.
KEGG	sce:YKL168C.
Organism-specific databases
SGD	S000001651. KKQ8.
Phylogenomic databases
eggNOG	COG0515.
GeneTree	ENSGT00550000075309.
HOGENOM	HOG000112846.
KO	K08286.
OrthoDB	EOG4J6W0P.
Gene expression databases
Genevestigator	P36004.
GermOnline	YKL168C. Saccharomyces cerevisiae.
Family and domain databases
InterPro	IPR011009. Kinase-like_dom. IPR000719. Prot_kinase_cat_dom. IPR008271. Ser/Thr_kinase_AS. [Graphical view]
Pfam	PF00069. Pkinase. 1 hit. [Graphical view]
SUPFAM	SSF56112. Kinase_like. 1 hit.
PROSITE	PS00107. PROTEIN_KINASE_ATP. False negative. PS50011. PROTEIN_KINASE_DOM. 1 hit. PS00108. PROTEIN_KINASE_ST. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	974655.

Entry information

Entry name

KKQ8_YEAST

Accession

Primary (citable) accession number: P36004
Secondary accession number(s): D6VX31

Entry history

Integrated into UniProtKB/Swiss-Prot:	June 1, 1994
Last sequence update:	May 20, 2008
Last modified:	May 1, 2013
This is version 118 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program