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P36004 (KKQ8_YEAST) Reviewed, UniProtKB/Swiss-Prot

Last modified May 14, 2014. Version 126. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Probable serine/threonine-protein kinase KKQ8

EC=2.7.11.1
Gene names
Name:KKQ8
Ordered Locus Names:YKL168C
ORF Names:YKL632
OrganismSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]
Taxonomic identifier559292 [NCBI]
Taxonomic lineageEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces

Protein attributes

Sequence length724 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Catalytic activity

ATP + a protein = ADP + a phosphoprotein.

Subcellular location

Cytoplasm Ref.5.

Miscellaneous

Present with 981 molecules/cell in log phase SD medium.

Sequence similarities

Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. NPR/HAL subfamily. HAL5 sub-subfamily.

Contains 1 protein kinase domain.

Sequence caution

The sequence CAA81519.1 differs from that shown. Reason: Erroneous initiation.

The sequence CAA82010.1 differs from that shown. Reason: Erroneous initiation.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 724724Probable serine/threonine-protein kinase KKQ8
PRO_0000086151

Regions

Domain412 – 712301Protein kinase
Nucleotide binding418 – 4269ATP By similarity

Sites

Active site5631Proton acceptor By similarity
Binding site4551ATP By similarity

Amino acid modifications

Modified residue191Phosphoserine Ref.9
Modified residue2321Phosphoserine Ref.8 Ref.10 Ref.11
Modified residue2381Phosphoserine Ref.8
Modified residue2411Phosphoserine Ref.8

Sequences

Sequence LengthMass (Da)Tools
P36004 [UniParc].

Last modified May 20, 2008. Version 2.
Checksum: 7EAC570CE6E5154F

FASTA72482,590
        10         20         30         40         50         60 
MVMQEEKKRQ QPVTRRVRSF SESFKNLFRP PRSRDSSPIN VTRIPYRSSS TSPKRSSEPP 

        70         80         90        100        110        120 
RRSTVSAQIL DPKNSPIRQR SYTLKCCTPG LSHPFRQTGS GASNSPTRHR SISGEEQEIV 

       130        140        150        160        170        180 
NSLPEYKRSA SHTFHGIRRP RSRSSSVSSC DSSNGTTSSS DSQWAMDSLL DDSDNDLTPY 

       190        200        210        220        230        240 
RGSNKDILKS KDRAPYNYID DYNKKALRRA TSYPNPLPSK QFYNERLYTR RSHPDEESLE 

       250        260        270        280        290        300 
SLPRFAGADV QCIIEQNGFK VYEDGSHEHN IKLSGVIAKL EKGNSLPAHR QGSLSRPRLG 

       310        320        330        340        350        360 
ITLSGLFKHH KNECDIENAL SLLPNVEKSQ TNHEKRTGQS PNDSNRSSPT QGREDYLKIV 

       370        380        390        400        410        420 
NPDASLGSDE LKLINSLSSR IHKSLQNYLQ EKNLKPAECI GEQAPTFQDN YGHPVGLVGA 

       430        440        450        460        470        480 
GAYGEVKLCA RLRNEKDSPP FETYHDSKYI YYAVKELKPK PDSDLEKFCT KITSEFIIGH 

       490        500        510        520        530        540 
SLSHYHKNGK KPAPNILNVF DILEDSSSFI EVMEFCPAGD LYGMLVGKSK LKGRLHPLEA 

       550        560        570        580        590        600 
DCFMKQLLHG VKFMHDHGIA HCDLKPENIL FYPHGLLKIC DFGTSSVFQT AWERRVHAQK 

       610        620        630        640        650        660 
GIIGSEPYVA PEEFVDGEYY DPRLIDCWSC GVVYITMILG HYLWKVASRE KDMSYDEFYK 

       670        680        690        700        710        720 
EMQRKNQFRV FEELKHVNSE LATNRKIALY RIFQWEPRKR ISVGKLLDMQ WMKSTNCCLI 


YDST 

« Hide

References

« Hide 'large scale' references
[1]"Sequencing and analysis of a 20.5 kb DNA segment located on the left arm of yeast chromosome XI."
Vandenbol M., Bolle P.-A., Dion C., Portetelle D., Hilger F.
Yeast 10:S25-S33(1994) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: ATCC 204508 / S288c.
[2]"Complete DNA sequence of yeast chromosome XI."
Dujon B., Alexandraki D., Andre B., Ansorge W., Baladron V., Ballesta J.P.G., Banrevi A., Bolle P.-A., Bolotin-Fukuhara M., Bossier P., Bou G., Boyer J., Buitrago M.J., Cheret G., Colleaux L., Daignan-Fornier B., del Rey F., Dion C. expand/collapse author list , Domdey H., Duesterhoeft A., Duesterhus S., Entian K.-D., Erfle H., Esteban P.F., Feldmann H., Fernandes L., Fobo G.M., Fritz C., Fukuhara H., Gabel C., Gaillon L., Garcia-Cantalejo J.M., Garcia-Ramirez J.J., Gent M.E., Ghazvini M., Goffeau A., Gonzalez A., Grothues D., Guerreiro P., Hegemann J.H., Hewitt N., Hilger F., Hollenberg C.P., Horaitis O., Indge K.J., Jacquier A., James C.M., Jauniaux J.-C., Jimenez A., Keuchel H., Kirchrath L., Kleine K., Koetter P., Legrain P., Liebl S., Louis E.J., Maia e Silva A., Marck C., Monnier A.-L., Moestl D., Mueller S., Obermaier B., Oliver S.G., Pallier C., Pascolo S., Pfeiffer F., Philippsen P., Planta R.J., Pohl F.M., Pohl T.M., Poehlmann R., Portetelle D., Purnelle B., Puzos V., Ramezani Rad M., Rasmussen S.W., Remacha M.A., Revuelta J.L., Richard G.-F., Rieger M., Rodrigues-Pousada C., Rose M., Rupp T., Santos M.A., Schwager C., Sensen C., Skala J., Soares H., Sor F., Stegemann J., Tettelin H., Thierry A., Tzermia M., Urrestarazu L.A., van Dyck L., van Vliet-Reedijk J.C., Valens M., Vandenbol M., Vilela C., Vissers S., von Wettstein D., Voss H., Wiemann S., Xu G., Zimmermann J., Haasemann M., Becker I., Mewes H.-W.
Nature 369:371-378(1994) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 204508 / S288c.
[3]"The reference genome sequence of Saccharomyces cerevisiae: Then and now."
Engel S.R., Dietrich F.S., Fisk D.G., Binkley G., Balakrishnan R., Costanzo M.C., Dwight S.S., Hitz B.C., Karra K., Nash R.S., Weng S., Wong E.D., Lloyd P., Skrzypek M.S., Miyasato S.R., Simison M., Cherry J.M.
G3 (Bethesda) 4:389-398(2014) [PubMed] [Europe PMC] [Abstract]
Cited for: GENOME REANNOTATION.
Strain: ATCC 204508 / S288c.
[4]"Sequencing and comparison of yeast species to identify genes and regulatory elements."
Kellis M., Patterson N., Endrizzi M., Birren B.W., Lander E.S.
Nature 423:241-254(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION OF PROBABLE INITIATION SITE.
[5]"Global analysis of protein localization in budding yeast."
Huh W.-K., Falvo J.V., Gerke L.C., Carroll A.S., Howson R.W., Weissman J.S., O'Shea E.K.
Nature 425:686-691(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
[6]"Global analysis of protein expression in yeast."
Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S.
Nature 425:737-741(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[7]"Finding functional features in Saccharomyces genomes by phylogenetic footprinting."
Cliften P.F., Sudarsanam P., Desikan A., Fulton L., Fulton B., Majors J., Waterston R., Cohen B.A., Johnston M.
Science 301:71-76(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION OF PROBABLE INITIATION SITE.
[8]"Large-scale phosphorylation analysis of alpha-factor-arrested Saccharomyces cerevisiae."
Li X., Gerber S.A., Rudner A.D., Beausoleil S.A., Haas W., Villen J., Elias J.E., Gygi S.P.
J. Proteome Res. 6:1190-1197(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-232; SER-238 AND SER-241, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
Strain: ADR376.
[9]"Analysis of phosphorylation sites on proteins from Saccharomyces cerevisiae by electron transfer dissociation (ETD) mass spectrometry."
Chi A., Huttenhower C., Geer L.Y., Coon J.J., Syka J.E.P., Bai D.L., Shabanowitz J., Burke D.J., Troyanskaya O.G., Hunt D.F.
Proc. Natl. Acad. Sci. U.S.A. 104:2193-2198(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-19, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
[10]"A multidimensional chromatography technology for in-depth phosphoproteome analysis."
Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.
Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-232, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
[11]"Global analysis of Cdk1 substrate phosphorylation sites provides insights into evolution."
Holt L.J., Tuch B.B., Villen J., Johnson A.D., Gygi S.P., Morgan D.O.
Science 325:1682-1686(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-232, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
Z26878 Genomic DNA. Translation: CAA81519.1. Different initiation.
Z28168 Genomic DNA. Translation: CAA82010.1. Different initiation.
BK006944 Genomic DNA. Translation: DAA08997.1.
PIRS37998.
RefSeqNP_012753.2. NM_001179734.1.

3D structure databases

ProteinModelPortalP36004.
SMRP36004. Positions 380-724.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid33969. 94 interactions.
DIPDIP-4838N.
IntActP36004. 6 interactions.
MINTMINT-473137.
STRING4932.YKL168C.

Proteomic databases

MaxQBP36004.
PaxDbP36004.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblFungiYKL168C; YKL168C; YKL168C.
GeneID853686.
KEGGsce:YKL168C.

Organism-specific databases

SGDS000001651. KKQ8.

Phylogenomic databases

eggNOGCOG0515.
GeneTreeENSGT00550000075309.
HOGENOMHOG000112846.
KOK08286.
OrthoDBEOG7008J9.

Enzyme and pathway databases

BioCycYEAST:G3O-31936-MONOMER.

Gene expression databases

GenevestigatorP36004.

Family and domain databases

InterProIPR011009. Kinase-like_dom.
IPR000719. Prot_kinase_dom.
IPR008271. Ser/Thr_kinase_AS.
[Graphical view]
PfamPF00069. Pkinase. 1 hit.
[Graphical view]
SUPFAMSSF56112. SSF56112. 1 hit.
PROSITEPS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio974655.

Entry information

Entry nameKKQ8_YEAST
AccessionPrimary (citable) accession number: P36004
Secondary accession number(s): D6VX31
Entry history
Integrated into UniProtKB/Swiss-Prot: June 1, 1994
Last sequence update: May 20, 2008
Last modified: May 14, 2014
This is version 126 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Relevant documents

Yeast chromosome XI

Yeast (Saccharomyces cerevisiae) chromosome XI: entries and gene names

Yeast

Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD

SIMILARITY comments

Index of protein domains and families