Reviewed, UniProtKB/Swiss-Prot P36003 (KKR1_YEAST)

Names and origin

Protein names

Recommended name:
    Probable serine/threonine-protein kinase YKL171W
    EC=2.7.11.1

Gene names

Ordered Locus Names:	YKL171W
ORF Names:	YKL635

Organism

Saccharomyces cerevisiae (Baker's yeast) [Complete proteome]

Taxonomic identifier

4932 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces

Protein attributes

Sequence length	928 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Catalytic activity	ATP + a protein = ADP + a phosphoprotein.
Sequence similarities	Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. Contains 1 protein kinase domain.

Ontologies

Keywords
Ligand	ATP-binding Nucleotide-binding
Molecular function	Kinase Serine/threonine-protein kinase Transferase
PTM	Phosphoprotein
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	protein amino acid phosphorylation Inferred from electronic annotation. Source: InterPro proteolysis Inferred from physical interaction. Source: SGD
Cellular component	cytoplasm Inferred from direct assay. Source: SGD
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW protein binding Inferred from physical interaction. Source: IntAct protein serine/threonine kinase activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
URE2	P23202	1	EBI-9796,EBI-20138

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 928	928	Probable serine/threonine-protein kinase YKL171W		PRO_0000086152
Regions
	Domain	449 – 912	464	Protein kinase
	Nucleotide binding	455 – 463	9	ATP By similarity
Sites
	Active site	580	1	Proton acceptor Potential
	Binding site	478	1	ATP By similarity
Amino acid modifications
	Modified residue	65	1	Phosphoserine Ref.3 Ref.4
	Modified residue	149	1	Phosphoserine Ref.4
	Modified residue	150	1	Phosphothreonine Ref.4
	Modified residue	179	1	Phosphoserine Ref.4
	Modified residue	415	1	Phosphoserine Ref.4
	Modified residue	426	1	Phosphoserine Ref.4
	Modified residue	636	1	Phosphoserine Ref.4
	Modified residue	689	1	Phosphoserine Ref.4
	Modified residue	737	1	Phosphoserine Ref.4
	Modified residue	739	1	Phosphotyrosine Ref.4
	Modified residue	742	1	Phosphoserine Ref.4

Sequences

	Sequence		Length	Mass (Da)
	P36003-1 [UniParc]. Last modified June 1, 1994. Version 1. Checksum: 00EB03553EC5999A	FASTA	928	103,956
	10 20 30 40 50 60 MFTSQRQLRQ NGSPMSSSRS SQHSSGTASP ISDSPASNRS YGRDLRGLMG IDIPANEPAF 70 80 90 100 110 120 NRANSSDTIY FRPKKIYKME HEHPSRSTLV QLQTRSQPDD VASSQVNPEG GTDDLELGDP 130 140 150 160 170 180 CGNQSLYTIG AEYVPDLDFT KLVNEWQKST EDLYEFRSSA TPQVQIKDSG KGNYELWSSP 190 200 210 220 230 240 DAILTQNKLR RDSFSQENSD SLSPEDSILS RNLHSKVKPI PLPRNSQQIF TPLSNLEAER 250 260 270 280 290 300 RSSYTTSSNN NSITQNNKFS FAKLKYSLPT QSSAVPASFD SNASSLNFLP TTTLSTLSEL 310 320 330 340 350 360 QISPNDMMDL IQKLPRNFLN LPYTQRKKVI IEHAPSHDYK AMMSLVKKFM LTSSRSNFSL 370 380 390 400 410 420 AGFANNASVS QATANDDNIN SRNTPNNSND TYVNTRPLQR SRHGSIASQF LSSFSPSMTS 430 440 450 460 470 480 IAKMNSNPLS GSAGGSARPD DKGMEILGHR LGKIIGFGAW GIIRECFDIE TGVGRVIKIV 490 500 510 520 530 540 KFKGHQNIKK HVLREVAIWR TLKHNRILPL LDWKLDDNYA MYCLTERIND GTLYDLVISW 550 560 570 580 590 600 DEFKRSKIPF AERCRLTIFL SLQLLSALKY MHSKTIVHGD IKLENCLLQK EGKKSDWKVF 610 620 630 640 650 660 LCDFGMSCHF DEKHVYRNDT FDENLSSGNS HRKRKSIEQT NLIKYPTTNF LPDDRTNDFD 670 680 690 700 710 720 ASENLKYQFE NRKHQPFTPK GMVSSSSHSL KHLNQPSSSS SSNLFHKPAS QPQPQHRSPF 730 740 750 760 770 780 HGRHKTTDFS NLEPEPSKYI GSLPYASPEL LRYSDARRSK SVEMHIYDSP DSSQSEISAA 790 800 810 820 830 840 SSSSSNLSSL SSSTKASAVT NSGVTTSSPS GSSTDFPCIV SPLGPASDIW ALGVMLYTML 850 860 870 880 890 900 VGKLPFNHEF EPRLRSLIKV GEFDRFSLAQ VCKFDRKKNE GTIGQGLYDT VIGCLTIDLD 910 920 KRWKLKRIEE VLQNEMNLSE AIHDNNGS

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Sequencing and analysis of a 20.5 kb DNA segment located on the left arm of yeast chromosome XI." Vandenbol M., Bolle P.-A., Dion C., Portetelle D., Hilger F. Yeast 10:S25-S33(1994) [PubMed: 8091858] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 204508 / S288c.
[2]	"Complete DNA sequence of yeast chromosome XI." Dujon B., Alexandraki D., Andre B., Ansorge W., Baladron V., Ballesta J.P.G., Banrevi A., Bolle P.-A., Bolotin-Fukuhara M., Bossier P., Bou G., Boyer J., Buitrago M.J., Cheret G., Colleaux L., Daignan-Fornier B., del Rey F., Dion C. , Domdey H., Duesterhoeft A., Duesterhus S., Entian K.-D., Erfle H., Esteban P.F., Feldmann H., Fernandes L., Fobo G.M., Fritz C., Fukuhara H., Gabel C., Gaillon L., Garcia-Cantalejo J.M., Garcia-Ramirez J.J., Gent M.E., Ghazvini M., Goffeau A., Gonzalez A., Grothues D., Guerreiro P., Hegemann J.H., Hewitt N., Hilger F., Hollenberg C.P., Horaitis O., Indge K.J., Jacquier A., James C.M., Jauniaux J.-C., Jimenez A., Keuchel H., Kirchrath L., Kleine K., Koetter P., Legrain P., Liebl S., Louis E.J., Maia e Silva A., Marck C., Monnier A.-L., Moestl D., Mueller S., Obermaier B., Oliver S.G., Pallier C., Pascolo S., Pfeiffer F., Philippsen P., Planta R.J., Pohl F.M., Pohl T.M., Poehlmann R., Portetelle D., Purnelle B., Puzos V., Ramezani Rad M., Rasmussen S.W., Remacha M.A., Revuelta J.L., Richard G.-F., Rieger M., Rodrigues-Pousada C., Rose M., Rupp T., Santos M.A., Schwager C., Sensen C., Skala J., Soares H., Sor F., Stegemann J., Tettelin H., Thierry A., Tzermia M., Urrestarazu L.A., van Dyck L., van Vliet-Reedijk J.C., Valens M., Vandenbol M., Vilela C., Vissers S., von Wettstein D., Voss H., Wiemann S., Xu G., Zimmermann J., Haasemann M., Becker I., Mewes H.-W. Nature 369:371-378(1994) [PubMed: 8196765] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 204508 / S288c.
[3]	"Proteome-wide identification of in vivo targets of DNA damage checkpoint kinases." Smolka M.B., Albuquerque C.P., Chen S.H., Zhou H. Proc. Natl. Acad. Sci. U.S.A. 104:10364-10369(2007) [PubMed: 17563356] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-65, MASS SPECTROMETRY.
[4]	"A multidimensional chromatography technology for in-depth phosphoproteome analysis." Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H. Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed: 18407956] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-65; SER-149; THR-150; SER-179; SER-415; SER-426; SER-636; SER-689; SER-737; TYR-739 AND SER-742, MASS SPECTROMETRY.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBLGenBankDDBJ	Z26878 Genomic DNA. Translation: CAA81516.1. Z28171 Genomic DNA. Translation: CAA82013.1.
PIR	S38001.
RefSeq	NP_012750.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
DIP	DIP:2877N.
IntAct	P36003. 7 interactions.
STRING	P36003.
Genome annotation databases
Ensembl	YKL171W; YKL171W; YKL171W; Saccharomyces cerevisiae. [Genome view]
GeneID	853683.
KEGG	sce:YKL171W.
NMPDR	fig|4932.3.peg.3728.
Organism-specific databases
CYGD	YKL171w.
SGD	S000001654. YKL171W.
Phylogenomic databases
HOGENOM	P36003.
OMA	SALKYMH
OrthoDB	EOG9CJX0V
Enzyme and pathway databases
BRENDA	2.7.11.1. 250.
Gene expression databases
ArrayExpress	P36003.
Genevestigator	P36003.
GermOnline	YKL171W. Saccharomyces cerevisiae.
Family and domain databases
InterPro	IPR020636. Ca/CaM-dep_prot_kinase-like. IPR011009. Kinase-like_dom. IPR000719. Prot_kinase_cat_dom. IPR017441. Protein_kinase_ATP_BS. IPR017442. Se/Thr_prot_kinase-like_dom. IPR016241. Ser/Thr_prot_kin_YKL171w_prd. IPR008271. Ser/Thr_prot_kinase_AS. [Graphical view]
PANTHER	PTHR22982. Ca/CaM-dep_prot_kinase-like. 1 hit.
Pfam	PF00069. Pkinase. 2 hits. [Graphical view]
PIRSF	PIRSF000610. Ser/Thr_PK_YKL171w_prd. 1 hit.
PROSITE	PS00107. PROTEIN_KINASE_ATP. 1 hit. PS50011. PROTEIN_KINASE_DOM. 1 hit. PS00108. PROTEIN_KINASE_ST. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	974649.

Entry information

Entry name

KKR1_YEAST

Accession

Primary (citable) accession number: P36003

Entry history

Integrated into UniProtKB/Swiss-Prot:	June 1, 1994
Last sequence update:	June 1, 1994
Last modified:	November 24, 2009
This is version 84 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

FPAP (Fungal Proteome Annotation Project)