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Protein

Nitrogen network kinase 1

Gene

NNK1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Serine/threonine-protein kinase involved in the phosphorylation of the NAD+-dependent glutamate dehydrogenase GDH2. When overexpressed, confers hypersensitivity to rapamycin and induces rapid nuclear accumulation of GLN3 to activate the transcription of nitrogen-regulated genes.1 Publication

Catalytic activityi

ATP + a protein = ADP + a phosphoprotein.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei478ATPPROSITE-ProRule annotation1
Active sitei580Proton acceptorPROSITE-ProRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi455 – 463ATPPROSITE-ProRule annotation9

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionKinase, Serine/threonine-protein kinase, Transferase
LigandATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciYEAST:G3O-31938-MONOMER

Names & Taxonomyi

Protein namesi
Recommended name:
Nitrogen network kinase 1 (EC:2.7.11.1)
Gene namesi
Name:NNK1
Ordered Locus Names:YKL171W
ORF Names:YKL635
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome XI

Organism-specific databases

EuPathDBiFungiDB:YKL171W
SGDiS000001654 NNK1

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000861521 – 928Nitrogen network kinase 1Add BLAST928

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei178PhosphoserineCombined sources1
Modified residuei179PhosphoserineCombined sources1
Modified residuei405PhosphoserineCombined sources1
Modified residuei426PhosphoserineCombined sources1
Modified residuei737PhosphoserineCombined sources1
Modified residuei739PhosphotyrosineCombined sources1

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiP36003
PaxDbiP36003
PRIDEiP36003

PTM databases

iPTMnetiP36003

Interactioni

Subunit structurei

Interacts with URE2 and GDH2. Interacts also with the TORC1 kinase complex.1 Publication

Binary interactionsi

Show more details

GO - Molecular functioni

Protein-protein interaction databases

BioGridi33967, 219 interactors
DIPiDIP-2877N
IntActiP36003, 21 interactors
MINTiP36003
STRINGi4932.YKL171W

Structurei

3D structure databases

ProteinModelPortaliP36003
SMRiP36003
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini449 – 912Protein kinasePROSITE-ProRule annotationAdd BLAST464

Sequence similaritiesi

Belongs to the protein kinase superfamily. Ser/Thr protein kinase family.PROSITE-ProRule annotation

Phylogenomic databases

InParanoidiP36003
KOiK08286
OMAiHSHIGSL
OrthoDBiEOG092C0H68

Family and domain databases

InterProiView protein in InterPro
IPR011009 Kinase-like_dom_sf
IPR016241 Nnk1
IPR000719 Prot_kinase_dom
IPR017441 Protein_kinase_ATP_BS
IPR008271 Ser/Thr_kinase_AS
PfamiView protein in Pfam
PF00069 Pkinase, 1 hit
PIRSFiPIRSF000610 Ser/Thr_PK_YKL171w_prd, 1 hit
SMARTiView protein in SMART
SM00220 S_TKc, 1 hit
SUPFAMiSSF56112 SSF56112, 3 hits
PROSITEiView protein in PROSITE
PS00107 PROTEIN_KINASE_ATP, 1 hit
PS50011 PROTEIN_KINASE_DOM, 1 hit
PS00108 PROTEIN_KINASE_ST, 1 hit

Sequencei

Sequence statusi: Complete.

P36003-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MFTSQRQLRQ NGSPMSSSRS SQHSSGTASP ISDSPASNRS YGRDLRGLMG
60 70 80 90 100
IDIPANEPAF NRANSSDTIY FRPKKIYKME HEHPSRSTLV QLQTRSQPDD
110 120 130 140 150
VASSQVNPEG GTDDLELGDP CGNQSLYTIG AEYVPDLDFT KLVNEWQKST
160 170 180 190 200
EDLYEFRSSA TPQVQIKDSG KGNYELWSSP DAILTQNKLR RDSFSQENSD
210 220 230 240 250
SLSPEDSILS RNLHSKVKPI PLPRNSQQIF TPLSNLEAER RSSYTTSSNN
260 270 280 290 300
NSITQNNKFS FAKLKYSLPT QSSAVPASFD SNASSLNFLP TTTLSTLSEL
310 320 330 340 350
QISPNDMMDL IQKLPRNFLN LPYTQRKKVI IEHAPSHDYK AMMSLVKKFM
360 370 380 390 400
LTSSRSNFSL AGFANNASVS QATANDDNIN SRNTPNNSND TYVNTRPLQR
410 420 430 440 450
SRHGSIASQF LSSFSPSMTS IAKMNSNPLS GSAGGSARPD DKGMEILGHR
460 470 480 490 500
LGKIIGFGAW GIIRECFDIE TGVGRVIKIV KFKGHQNIKK HVLREVAIWR
510 520 530 540 550
TLKHNRILPL LDWKLDDNYA MYCLTERIND GTLYDLVISW DEFKRSKIPF
560 570 580 590 600
AERCRLTIFL SLQLLSALKY MHSKTIVHGD IKLENCLLQK EGKKSDWKVF
610 620 630 640 650
LCDFGMSCHF DEKHVYRNDT FDENLSSGNS HRKRKSIEQT NLIKYPTTNF
660 670 680 690 700
LPDDRTNDFD ASENLKYQFE NRKHQPFTPK GMVSSSSHSL KHLNQPSSSS
710 720 730 740 750
SSNLFHKPAS QPQPQHRSPF HGRHKTTDFS NLEPEPSKYI GSLPYASPEL
760 770 780 790 800
LRYSDARRSK SVEMHIYDSP DSSQSEISAA SSSSSNLSSL SSSTKASAVT
810 820 830 840 850
NSGVTTSSPS GSSTDFPCIV SPLGPASDIW ALGVMLYTML VGKLPFNHEF
860 870 880 890 900
EPRLRSLIKV GEFDRFSLAQ VCKFDRKKNE GTIGQGLYDT VIGCLTIDLD
910 920
KRWKLKRIEE VLQNEMNLSE AIHDNNGS
Length:928
Mass (Da):103,956
Last modified:June 1, 1994 - v1
Checksum:i00EB03553EC5999A
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z26878 Genomic DNA Translation: CAA81516.1
Z28171 Genomic DNA Translation: CAA82013.1
BK006944 Genomic DNA Translation: DAA08995.1
PIRiS38001
RefSeqiNP_012750.1, NM_001179737.1

Genome annotation databases

EnsemblFungiiYKL171W; YKL171W; YKL171W
GeneIDi853683
KEGGisce:YKL171W

Similar proteinsi

Entry informationi

Entry nameiNNK1_YEAST
AccessioniPrimary (citable) accession number: P36003
Secondary accession number(s): D6VX29
Entry historyiIntegrated into UniProtKB/Swiss-Prot: June 1, 1994
Last sequence update: June 1, 1994
Last modified: May 23, 2018
This is version 152 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health