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Protein

Nitrogen network kinase 1

Gene

NNK1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Serine/threonine-protein kinase involved in the phosphorylation of the NAD+-dependent glutamate dehydrogenase GDH2. When overexpressed, confers hypersensitivity to rapamycin and induces rapid nuclear accumulation of GLN3 to activate the transcription of nitrogen-regulated genes.1 Publication

Catalytic activityi

ATP + a protein = ADP + a phosphoprotein.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei478 – 4781ATPPROSITE-ProRule annotation
Active sitei580 – 5801Proton acceptorPROSITE-ProRule annotation

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi455 – 4639ATPPROSITE-ProRule annotation

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Kinase, Serine/threonine-protein kinase, Transferase

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciYEAST:G3O-31938-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Nitrogen network kinase 1 (EC:2.7.11.1)
Gene namesi
Name:NNK1
Ordered Locus Names:YKL171W
ORF Names:YKL635
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome XI

Organism-specific databases

EuPathDBiFungiDB:YKL171W.
SGDiS000001654. NNK1.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 928928Nitrogen network kinase 1PRO_0000086152Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei178 – 1781PhosphoserineCombined sources
Modified residuei179 – 1791PhosphoserineCombined sources
Modified residuei405 – 4051PhosphoserineCombined sources
Modified residuei426 – 4261PhosphoserineCombined sources
Modified residuei737 – 7371PhosphoserineCombined sources
Modified residuei739 – 7391PhosphotyrosineCombined sources

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiP36003.
PRIDEiP36003.

PTM databases

iPTMnetiP36003.

Interactioni

Subunit structurei

Interacts with URE2 and GDH2. Interacts also with the TORC1 kinase complex.1 Publication

Binary interactionsi

WithEntry#Exp.IntActNotes
GDH2P333273EBI-9796,EBI-5815
HRR25P292954EBI-9796,EBI-8536
TOR1P351693EBI-9796,EBI-19374
URE2P232024EBI-9796,EBI-20138

GO - Molecular functioni

Protein-protein interaction databases

BioGridi33967. 98 interactions.
DIPiDIP-2877N.
IntActiP36003. 20 interactions.
MINTiMINT-513221.

Structurei

3D structure databases

ProteinModelPortaliP36003.
SMRiP36003. Positions 453-731.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini449 – 912464Protein kinasePROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Belongs to the protein kinase superfamily. Ser/Thr protein kinase family.PROSITE-ProRule annotation
Contains 1 protein kinase domain.PROSITE-ProRule annotation

Phylogenomic databases

InParanoidiP36003.
KOiK08286.
OMAiMYCLTER.
OrthoDBiEOG7ZSJ29.

Family and domain databases

InterProiIPR011009. Kinase-like_dom.
IPR016241. Nnk1.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR008271. Ser/Thr_kinase_AS.
[Graphical view]
PfamiPF00069. Pkinase. 1 hit.
[Graphical view]
PIRSFiPIRSF000610. Ser/Thr_PK_YKL171w_prd. 1 hit.
SMARTiSM00220. S_TKc. 1 hit.
[Graphical view]
SUPFAMiSSF56112. SSF56112. 3 hits.
PROSITEiPS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P36003-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MFTSQRQLRQ NGSPMSSSRS SQHSSGTASP ISDSPASNRS YGRDLRGLMG
60 70 80 90 100
IDIPANEPAF NRANSSDTIY FRPKKIYKME HEHPSRSTLV QLQTRSQPDD
110 120 130 140 150
VASSQVNPEG GTDDLELGDP CGNQSLYTIG AEYVPDLDFT KLVNEWQKST
160 170 180 190 200
EDLYEFRSSA TPQVQIKDSG KGNYELWSSP DAILTQNKLR RDSFSQENSD
210 220 230 240 250
SLSPEDSILS RNLHSKVKPI PLPRNSQQIF TPLSNLEAER RSSYTTSSNN
260 270 280 290 300
NSITQNNKFS FAKLKYSLPT QSSAVPASFD SNASSLNFLP TTTLSTLSEL
310 320 330 340 350
QISPNDMMDL IQKLPRNFLN LPYTQRKKVI IEHAPSHDYK AMMSLVKKFM
360 370 380 390 400
LTSSRSNFSL AGFANNASVS QATANDDNIN SRNTPNNSND TYVNTRPLQR
410 420 430 440 450
SRHGSIASQF LSSFSPSMTS IAKMNSNPLS GSAGGSARPD DKGMEILGHR
460 470 480 490 500
LGKIIGFGAW GIIRECFDIE TGVGRVIKIV KFKGHQNIKK HVLREVAIWR
510 520 530 540 550
TLKHNRILPL LDWKLDDNYA MYCLTERIND GTLYDLVISW DEFKRSKIPF
560 570 580 590 600
AERCRLTIFL SLQLLSALKY MHSKTIVHGD IKLENCLLQK EGKKSDWKVF
610 620 630 640 650
LCDFGMSCHF DEKHVYRNDT FDENLSSGNS HRKRKSIEQT NLIKYPTTNF
660 670 680 690 700
LPDDRTNDFD ASENLKYQFE NRKHQPFTPK GMVSSSSHSL KHLNQPSSSS
710 720 730 740 750
SSNLFHKPAS QPQPQHRSPF HGRHKTTDFS NLEPEPSKYI GSLPYASPEL
760 770 780 790 800
LRYSDARRSK SVEMHIYDSP DSSQSEISAA SSSSSNLSSL SSSTKASAVT
810 820 830 840 850
NSGVTTSSPS GSSTDFPCIV SPLGPASDIW ALGVMLYTML VGKLPFNHEF
860 870 880 890 900
EPRLRSLIKV GEFDRFSLAQ VCKFDRKKNE GTIGQGLYDT VIGCLTIDLD
910 920
KRWKLKRIEE VLQNEMNLSE AIHDNNGS
Length:928
Mass (Da):103,956
Last modified:June 1, 1994 - v1
Checksum:i00EB03553EC5999A
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z26878 Genomic DNA. Translation: CAA81516.1.
Z28171 Genomic DNA. Translation: CAA82013.1.
BK006944 Genomic DNA. Translation: DAA08995.1.
PIRiS38001.
RefSeqiNP_012750.1. NM_001179737.1.

Genome annotation databases

EnsemblFungiiYKL171W; YKL171W; YKL171W.
GeneIDi853683.
KEGGisce:YKL171W.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z26878 Genomic DNA. Translation: CAA81516.1.
Z28171 Genomic DNA. Translation: CAA82013.1.
BK006944 Genomic DNA. Translation: DAA08995.1.
PIRiS38001.
RefSeqiNP_012750.1. NM_001179737.1.

3D structure databases

ProteinModelPortaliP36003.
SMRiP36003. Positions 453-731.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi33967. 98 interactions.
DIPiDIP-2877N.
IntActiP36003. 20 interactions.
MINTiMINT-513221.

PTM databases

iPTMnetiP36003.

Proteomic databases

MaxQBiP36003.
PRIDEiP36003.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYKL171W; YKL171W; YKL171W.
GeneIDi853683.
KEGGisce:YKL171W.

Organism-specific databases

EuPathDBiFungiDB:YKL171W.
SGDiS000001654. NNK1.

Phylogenomic databases

InParanoidiP36003.
KOiK08286.
OMAiMYCLTER.
OrthoDBiEOG7ZSJ29.

Enzyme and pathway databases

BioCyciYEAST:G3O-31938-MONOMER.

Miscellaneous databases

PROiP36003.

Family and domain databases

InterProiIPR011009. Kinase-like_dom.
IPR016241. Nnk1.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR008271. Ser/Thr_kinase_AS.
[Graphical view]
PfamiPF00069. Pkinase. 1 hit.
[Graphical view]
PIRSFiPIRSF000610. Ser/Thr_PK_YKL171w_prd. 1 hit.
SMARTiSM00220. S_TKc. 1 hit.
[Graphical view]
SUPFAMiSSF56112. SSF56112. 3 hits.
PROSITEiPS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Sequencing and analysis of a 20.5 kb DNA segment located on the left arm of yeast chromosome XI."
    Vandenbol M., Bolle P.-A., Dion C., Portetelle D., Hilger F.
    Yeast 10:S25-S33(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  2. "Complete DNA sequence of yeast chromosome XI."
    Dujon B., Alexandraki D., Andre B., Ansorge W., Baladron V., Ballesta J.P.G., Banrevi A., Bolle P.-A., Bolotin-Fukuhara M., Bossier P., Bou G., Boyer J., Buitrago M.J., Cheret G., Colleaux L., Daignan-Fornier B., del Rey F., Dion C.
    , Domdey H., Duesterhoeft A., Duesterhus S., Entian K.-D., Erfle H., Esteban P.F., Feldmann H., Fernandes L., Fobo G.M., Fritz C., Fukuhara H., Gabel C., Gaillon L., Garcia-Cantalejo J.M., Garcia-Ramirez J.J., Gent M.E., Ghazvini M., Goffeau A., Gonzalez A., Grothues D., Guerreiro P., Hegemann J.H., Hewitt N., Hilger F., Hollenberg C.P., Horaitis O., Indge K.J., Jacquier A., James C.M., Jauniaux J.-C., Jimenez A., Keuchel H., Kirchrath L., Kleine K., Koetter P., Legrain P., Liebl S., Louis E.J., Maia e Silva A., Marck C., Monnier A.-L., Moestl D., Mueller S., Obermaier B., Oliver S.G., Pallier C., Pascolo S., Pfeiffer F., Philippsen P., Planta R.J., Pohl F.M., Pohl T.M., Poehlmann R., Portetelle D., Purnelle B., Puzos V., Ramezani Rad M., Rasmussen S.W., Remacha M.A., Revuelta J.L., Richard G.-F., Rieger M., Rodrigues-Pousada C., Rose M., Rupp T., Santos M.A., Schwager C., Sensen C., Skala J., Soares H., Sor F., Stegemann J., Tettelin H., Thierry A., Tzermia M., Urrestarazu L.A., van Dyck L., van Vliet-Reedijk J.C., Valens M., Vandenbol M., Vilela C., Vissers S., von Wettstein D., Voss H., Wiemann S., Xu G., Zimmermann J., Haasemann M., Becker I., Mewes H.-W.
    Nature 369:371-378(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  3. Cited for: GENOME REANNOTATION.
    Strain: ATCC 204508 / S288c.
  4. Cited for: SUBCELLULAR LOCATION.
  5. "Classification and functional annotation of eukaryotic protein kinases."
    Miranda-Saavedra D., Barton G.J.
    Proteins 68:893-914(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: PREDICTION OF FUNCTION.
  6. "A multidimensional chromatography technology for in-depth phosphoproteome analysis."
    Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.
    Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-737 AND TYR-739, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  7. "Global analysis of Cdk1 substrate phosphorylation sites provides insights into evolution."
    Holt L.J., Tuch B.B., Villen J., Johnson A.D., Gygi S.P., Morgan D.O.
    Science 325:1682-1686(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-178; SER-179; SER-405; SER-426; SER-737 AND TYR-739, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  8. Cited for: FUNCTION, INTERACTION WITH URE2; GDH2 AND THE TORC1 COMPLEX.

Entry informationi

Entry nameiNNK1_YEAST
AccessioniPrimary (citable) accession number: P36003
Secondary accession number(s): D6VX29
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 1, 1994
Last sequence update: June 1, 1994
Last modified: July 6, 2016
This is version 137 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  3. Yeast chromosome XI
    Yeast (Saccharomyces cerevisiae) chromosome XI: entries and gene names

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.