Reviewed, UniProtKB/Swiss-Prot P36002 (PTK1_YEAST)

Names and origin

Protein names

Recommended name:
    Serine/threonine-protein kinase PTK1/STK1
    EC=2.7.11.1

Gene names

Name:	PTK1
Synonyms:	POT1, STK1
Ordered Locus Names:	YKL198C/YKL199C

Organism

Saccharomyces cerevisiae (Baker's yeast) [Complete proteome]

Taxonomic identifier

4932 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces

Protein attributes

Sequence length	649 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Essential determinant for low-affinity spermidine transport.
Catalytic activity	ATP + a protein = ADP + a phosphoprotein.
Sequence similarities	Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. Contains 1 protein kinase domain.
Sequence caution	The sequence BAA09884.1 differs from that shown. Reason: Frameshift at position 455. The sequence CAA82042.1 differs from that shown. Reason: Frameshift at position 455. The sequence CAA82043.1 differs from that shown. Reason: Frameshift at position 455.

Ontologies

Keywords
Ligand	ATP-binding Nucleotide-binding
Molecular function	Kinase Serine/threonine-protein kinase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	polyamine transport Ref.2 Inferred from genetic interaction. Source: SGD protein amino acid phosphorylation Inferred from electronic annotation. Source: InterPro
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW protein serine/threonine kinase activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 649	649	Serine/threonine-protein kinase PTK1/STK1		PRO_0000086589
Regions
	Domain	196 – 503	308	Protein kinase
	Nucleotide binding	202 – 210	9	ATP By similarity
	Compositional bias	4 – 146	143	Ser-rich
	Compositional bias	567 – 624	58	Pro-rich
Sites
	Active site	329	1	Proton acceptor By similarity
	Binding site	226	1	ATP By similarity
Experimental info
	Sequence conflict	241 – 244	4	NAAP → KRCS in BAA09884. Ref.2
	Sequence conflict	300	1	H → S in BAA09884. Ref.2
	Sequence conflict	356 – 375	20	TDPHD…MIGSP → HGSTRPVQPCQEVRRDDRLA in CAA82043. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	P36002-1 [UniParc]. Last modified October 25, 2004. Version 2. Checksum: A07BBC47382DA9F5	FASTA	649	72,062
	10 20 30 40 50 60 MTVSHNHSTK ISQQPISSVS AFKFFGKKLL SSSHGNKLKK KASLPPDFHS TSTNDSESSS 70 80 90 100 110 120 PKLPNSLKTS RRANSFAHTT NSKRSLSSAS TKILPPAGSS TSISRGNRHS STSRNLSNSK 130 140 150 160 170 180 FSSERLVYNP YGVSTPSTSL SSVSTSMKKD PDLGFYLHDG DSKIRMLPIP IVDPNEYLPD 190 200 210 220 230 240 EMKEASIQLS DNFVFDDENK TIGWGGSCEV RKIRSKYRKK DVFALKKLNM IYNETPEKFY 250 260 270 280 290 300 NAAPKEFIIA KQLSHHVHIT NTFLLVKVPT TVYTTRGWGF VMELGLRDLF AMIQKSGWRH 310 320 330 340 350 360 VALAEKFCIF KQVACGVKFC HDQGIAHRDL KPENVLLSPD GVCKLTDFGI SDWYHTDPHD 370 380 390 400 410 420 LSSPVKKCAG MIGSPPYAPP EVMFYDSKKH YDTELQQPYD PRALDCYGLG IILMTLVNNV 430 440 450 460 470 480 IPFLESCSFD TGFRDYCDAY ENFIRLHDRA FRNRGNYRPG PGMEYHLARN FKNGHASRVA 490 500 510 520 530 540 WRLADPEAAT RYTIDDLFED PWFQGIETCV DANDKYVCKK PIIKTTTYEN PRGFHIATDV 550 560 570 580 590 600 AATTPTSNPF LKNRVPIRSM VDIAAHPSPT ATVLASSPPP PPPATHVPAE ALFTLRETPP 610 620 630 640 PQLATLTLSE EPPATPAPSA PSAPSARVRG HSPHRVGASP SEHCQQLGP

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Complete DNA sequence of yeast chromosome XI." Dujon B., Alexandraki D., Andre B., Ansorge W., Baladron V., Ballesta J.P.G., Banrevi A., Bolle P.-A., Bolotin-Fukuhara M., Bossier P., Bou G., Boyer J., Buitrago M.J., Cheret G., Colleaux L., Daignan-Fornier B., del Rey F., Dion C. , Domdey H., Duesterhoeft A., Duesterhus S., Entian K.-D., Erfle H., Esteban P.F., Feldmann H., Fernandes L., Fobo G.M., Fritz C., Fukuhara H., Gabel C., Gaillon L., Garcia-Cantalejo J.M., Garcia-Ramirez J.J., Gent M.E., Ghazvini M., Goffeau A., Gonzalez A., Grothues D., Guerreiro P., Hegemann J.H., Hewitt N., Hilger F., Hollenberg C.P., Horaitis O., Indge K.J., Jacquier A., James C.M., Jauniaux J.-C., Jimenez A., Keuchel H., Kirchrath L., Kleine K., Koetter P., Legrain P., Liebl S., Louis E.J., Maia e Silva A., Marck C., Monnier A.-L., Moestl D., Mueller S., Obermaier B., Oliver S.G., Pallier C., Pascolo S., Pfeiffer F., Philippsen P., Planta R.J., Pohl F.M., Pohl T.M., Poehlmann R., Portetelle D., Purnelle B., Puzos V., Ramezani Rad M., Rasmussen S.W., Remacha M.A., Revuelta J.L., Richard G.-F., Rieger M., Rodrigues-Pousada C., Rose M., Rupp T., Santos M.A., Schwager C., Sensen C., Skala J., Soares H., Sor F., Stegemann J., Tettelin H., Thierry A., Tzermia M., Urrestarazu L.A., van Dyck L., van Vliet-Reedijk J.C., Valens M., Vandenbol M., Vilela C., Vissers S., von Wettstein D., Voss H., Wiemann S., Xu G., Zimmermann J., Haasemann M., Becker I., Mewes H.-W. Nature 369:371-378(1994) [PubMed: 8196765] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 96604 / S288c / FY1679.
[2]	"Cloning of the gene encoding a putative serine/threonine protein kinase which enhances spermine uptake in Saccharomyces cerevisiae." Kakinuma Y., Maruyama T., Nozaki T., Wada Y., Ohsumi Y., Igarashi K. Biochem. Biophys. Res. Commun. 216:985-992(1995) [PubMed: 7488221] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-584, CHARACTERIZATION.
[3]	"The STK2 gene, which encodes a putative Ser/Thr protein kinase, is required for high-affinity spermidine transport in Saccharomyces cerevisiae." Kaouass M., Audette M., Ramotar D., Verma S., de Montigny D., Gamache I., Torossian K., Poulin R. Mol. Cell. Biol. 17:2994-3004(1997) [PubMed: 9154797] [Abstract] Cited for: CHARACTERIZATION.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBLGenBankDDBJ	Z28198 Genomic DNA. Translation: CAA82043.1. Frameshift. Z28198 Genomic DNA. Translation: CAA82042.1. Frameshift. D63815 Genomic DNA. Translation: BAA09884.1. Frameshift.
PIR	S38035. S38036.
RefSeq	NP_012723.2.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
IntAct	P36002. 2 interactions.
STRING	P36002.
Proteomic databases
PRIDE	P36002.
Genome annotation databases
Ensembl	YKL198C; YKL198C; YKL198C; Saccharomyces cerevisiae. [Genome view]
GeneID	853635.
KEGG	sce:YKL198C.
NMPDR	fig|4932.3.peg.3700.
Organism-specific databases
CYGD	YKL198c.
SGD	S000001681. PTK1.
Phylogenomic databases
HOGENOM	P36002.
OMA	ACIRNIN
OrthoDB	EOG9NW12S
Enzyme and pathway databases
BRENDA	2.7.11.1. 250.
Gene expression databases
ArrayExpress	P36002.
Genevestigator	P36002.
GermOnline	YKL198C. Saccharomyces cerevisiae.
Family and domain databases
InterPro	IPR020636. Ca/CaM-dep_prot_kinase-like. IPR011009. Kinase-like_dom. IPR000719. Prot_kinase_cat_dom. IPR017442. Se/Thr_prot_kinase-like_dom. IPR008271. Ser/Thr_prot_kinase_AS. [Graphical view]
PANTHER	PTHR22982. Ca/CaM-dep_prot_kinase-like. 1 hit.
Pfam	PF00069. Pkinase. 1 hit. [Graphical view]
PROSITE	PS00107. PROTEIN_KINASE_ATP. False negative. PS50011. PROTEIN_KINASE_DOM. 1 hit. PS00108. PROTEIN_KINASE_ST. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	974517.

Entry information

Entry name

PTK1_YEAST

Accession

Primary (citable) accession number: P36002
Secondary accession number(s): P36045, Q06941

Entry history

Integrated into UniProtKB/Swiss-Prot:	June 1, 1994
Last sequence update:	October 25, 2004
Last modified:	November 24, 2009
This is version 83 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

FPAP (Fungal Proteome Annotation Project)