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Reviewed, UniProtKB/Swiss-Prot P36002 (PTK1_YEAST)

Last modified November 24, 2009. Version 83. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data | Customize display text xml rdf/xml gff fasta
Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents

Names and origin

Protein namesRecommended name:
    Serine/threonine-protein kinase PTK1/STK1
    EC=2.7.11.1
Gene names
Name: PTK1
Synonyms: POT1, STK1
Ordered Locus Names: YKL198C/YKL199C
OrganismSaccharomyces cerevisiae (Baker's yeast) [Complete proteome]
Taxonomic identifier4932 [NCBI]
Taxonomic lineageEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces

Protein attributes

Sequence length649 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is not processed.
Protein existenceEvidence at protein level.

General annotation (Comments)

Function

Essential determinant for low-affinity spermidine transport.

Catalytic activity

ATP + a protein = ADP + a phosphoprotein.

Sequence similarities

Belongs to the protein kinase superfamily. Ser/Thr protein kinase family.

Contains 1 protein kinase domain.

Sequence caution

The sequence BAA09884.1 differs from that shown. Reason: Frameshift at position 455.

The sequence CAA82042.1 differs from that shown. Reason: Frameshift at position 455.

The sequence CAA82043.1 differs from that shown. Reason: Frameshift at position 455.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 649649Serine/threonine-protein kinase PTK1/STK1
PRO_0000086589

Regions

Domain196 – 503308Protein kinase
Nucleotide binding202 – 2109ATP By similarity
Compositional bias4 – 146143Ser-rich
Compositional bias567 – 62458Pro-rich

Sites

Active site3291Proton acceptor By similarity
Binding site2261ATP By similarity

Experimental info

Sequence conflict241 – 2444NAAP → KRCS in BAA09884. Ref.2
Sequence conflict3001H → S in BAA09884. Ref.2
Sequence conflict356 – 37520TDPHD…MIGSP → HGSTRPVQPCQEVRRDDRLA in CAA82043. Ref.1

Sequences

Sequence LengthMass (Da)Tools
P36002-1 [UniParc].

Last modified October 25, 2004. Version 2.
Checksum: A07BBC47382DA9F5

FASTA64972,062
        10         20         30         40         50         60 
MTVSHNHSTK ISQQPISSVS AFKFFGKKLL SSSHGNKLKK KASLPPDFHS TSTNDSESSS 

        70         80         90        100        110        120 
PKLPNSLKTS RRANSFAHTT NSKRSLSSAS TKILPPAGSS TSISRGNRHS STSRNLSNSK 

       130        140        150        160        170        180 
FSSERLVYNP YGVSTPSTSL SSVSTSMKKD PDLGFYLHDG DSKIRMLPIP IVDPNEYLPD 

       190        200        210        220        230        240 
EMKEASIQLS DNFVFDDENK TIGWGGSCEV RKIRSKYRKK DVFALKKLNM IYNETPEKFY 

       250        260        270        280        290        300 
NAAPKEFIIA KQLSHHVHIT NTFLLVKVPT TVYTTRGWGF VMELGLRDLF AMIQKSGWRH 

       310        320        330        340        350        360 
VALAEKFCIF KQVACGVKFC HDQGIAHRDL KPENVLLSPD GVCKLTDFGI SDWYHTDPHD 

       370        380        390        400        410        420 
LSSPVKKCAG MIGSPPYAPP EVMFYDSKKH YDTELQQPYD PRALDCYGLG IILMTLVNNV 

       430        440        450        460        470        480 
IPFLESCSFD TGFRDYCDAY ENFIRLHDRA FRNRGNYRPG PGMEYHLARN FKNGHASRVA 

       490        500        510        520        530        540 
WRLADPEAAT RYTIDDLFED PWFQGIETCV DANDKYVCKK PIIKTTTYEN PRGFHIATDV 

       550        560        570        580        590        600 
AATTPTSNPF LKNRVPIRSM VDIAAHPSPT ATVLASSPPP PPPATHVPAE ALFTLRETPP 

       610        620        630        640 
PQLATLTLSE EPPATPAPSA PSAPSARVRG HSPHRVGASP SEHCQQLGP 

« Hide

References

« Hide 'large scale' references
[1]"Complete DNA sequence of yeast chromosome XI."
Dujon B., Alexandraki D., Andre B., Ansorge W., Baladron V., Ballesta J.P.G., Banrevi A., Bolle P.-A., Bolotin-Fukuhara M., Bossier P., Bou G., Boyer J., Buitrago M.J., Cheret G., Colleaux L., Daignan-Fornier B., del Rey F., Dion C. expand/collapse author list , Domdey H., Duesterhoeft A., Duesterhus S., Entian K.-D., Erfle H., Esteban P.F., Feldmann H., Fernandes L., Fobo G.M., Fritz C., Fukuhara H., Gabel C., Gaillon L., Garcia-Cantalejo J.M., Garcia-Ramirez J.J., Gent M.E., Ghazvini M., Goffeau A., Gonzalez A., Grothues D., Guerreiro P., Hegemann J.H., Hewitt N., Hilger F., Hollenberg C.P., Horaitis O., Indge K.J., Jacquier A., James C.M., Jauniaux J.-C., Jimenez A., Keuchel H., Kirchrath L., Kleine K., Koetter P., Legrain P., Liebl S., Louis E.J., Maia e Silva A., Marck C., Monnier A.-L., Moestl D., Mueller S., Obermaier B., Oliver S.G., Pallier C., Pascolo S., Pfeiffer F., Philippsen P., Planta R.J., Pohl F.M., Pohl T.M., Poehlmann R., Portetelle D., Purnelle B., Puzos V., Ramezani Rad M., Rasmussen S.W., Remacha M.A., Revuelta J.L., Richard G.-F., Rieger M., Rodrigues-Pousada C., Rose M., Rupp T., Santos M.A., Schwager C., Sensen C., Skala J., Soares H., Sor F., Stegemann J., Tettelin H., Thierry A., Tzermia M., Urrestarazu L.A., van Dyck L., van Vliet-Reedijk J.C., Valens M., Vandenbol M., Vilela C., Vissers S., von Wettstein D., Voss H., Wiemann S., Xu G., Zimmermann J., Haasemann M., Becker I., Mewes H.-W.
Nature 369:371-378(1994) [PubMed: 8196765] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 96604 / S288c / FY1679.
[2]"Cloning of the gene encoding a putative serine/threonine protein kinase which enhances spermine uptake in Saccharomyces cerevisiae."
Kakinuma Y., Maruyama T., Nozaki T., Wada Y., Ohsumi Y., Igarashi K.
Biochem. Biophys. Res. Commun. 216:985-992(1995) [PubMed: 7488221] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-584, CHARACTERIZATION.
[3]"The STK2 gene, which encodes a putative Ser/Thr protein kinase, is required for high-affinity spermidine transport in Saccharomyces cerevisiae."
Kaouass M., Audette M., Ramotar D., Verma S., de Montigny D., Gamache I., Torossian K., Poulin R.
Mol. Cell. Biol. 17:2994-3004(1997) [PubMed: 9154797] [Abstract]
Cited for: CHARACTERIZATION.
+Additional computationally mapped references.

Cross-references

Sequence databases

Z28198 Genomic DNA. Translation: CAA82043.1. Frameshift.
Z28198 Genomic DNA. Translation: CAA82042.1. Frameshift.
D63815 Genomic DNA. Translation: BAA09884.1. Frameshift.
PIRS38035.
S38036.
RefSeqNP_012723.2.

3D structure databases

ModBaseSearch...

Protein-protein interaction databases

IntActP36002. 2 interactions.
STRINGP36002.

Proteomic databases

PRIDEP36002.

Genome annotation databases

EnsemblYKL198C; YKL198C; YKL198C; Saccharomyces cerevisiae. [Genome view]
GeneID853635.
KEGGsce:YKL198C.
NMPDRfig|4932.3.peg.3700.

Organism-specific databases

CYGDYKL198c.
SGDS000001681. PTK1.

Phylogenomic databases

HOGENOMP36002.
OMAACIRNIN
OrthoDBEOG9NW12S

Enzyme and pathway databases

BRENDA2.7.11.1. 250.

Gene expression databases

ArrayExpressP36002.
GenevestigatorP36002.
GermOnlineYKL198C. Saccharomyces cerevisiae.

Family and domain databases

InterProIPR020636. Ca/CaM-dep_prot_kinase-like.
IPR011009. Kinase-like_dom.
IPR000719. Prot_kinase_cat_dom.
IPR017442. Se/Thr_prot_kinase-like_dom.
IPR008271. Ser/Thr_prot_kinase_AS.
[Graphical view]
PANTHERPTHR22982. Ca/CaM-dep_prot_kinase-like. 1 hit.
PfamPF00069. Pkinase. 1 hit.
[Graphical view]
PROSITEPS00107. PROTEIN_KINASE_ATP. False negative.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
[Graphical view]
ProtoNetSearch...

Other Resources

NextBio974517.

Entry information

Entry namePTK1_YEAST
AccessionPrimary (citable) accession number: P36002
Secondary accession number(s): P36045, Q06941
Entry history
Integrated into UniProtKB/Swiss-Prot: June 1, 1994
Last sequence update: October 25, 2004
Last modified: November 24, 2009
This is version 83 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation projectFPAP (Fungal Proteome Annotation Project)

Relevant documents

SIMILARITY comments

Index of protein domains and families

Yeast

Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD

Yeast chromosome XI

Yeast (Saccharomyces cerevisiae) chromosome XI: entries and gene names

Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents