P36002 (PTK1_YEAST) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Serine/threonine-protein kinase PTK1/STK1
EC=2.7.11.1

Gene names

Name:	PTK1
Synonyms:	POT1, STK1
Ordered Locus Names:	YKL198C/YKL199C

Organism

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)

Taxonomic identifier

559292 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces

Protein attributes

Sequence length	662 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Essential determinant for low-affinity spermidine transport.
Catalytic activity	ATP + a protein = ADP + a phosphoprotein.
Sequence similarities	Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. Contains 1 protein kinase domain.
Sequence caution	The sequence BAA09884.1 differs from that shown. Reason: Frameshift at position 455. The sequence CAA82042.1 differs from that shown. Reason: Frameshift at position 455. The sequence CAA82043.1 differs from that shown. Reason: Frameshift at position 455.

Ontologies

Keywords
Ligand	ATP-binding Nucleotide-binding
Molecular function	Kinase Serine/threonine-protein kinase Transferase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	polyamine transport Inferred from mutant phenotype Ref.3. Source: SGD
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW protein serine/threonine kinase activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 662	662	Serine/threonine-protein kinase PTK1/STK1		PRO_0000086589
Regions
	Domain	196 – 503	308	Protein kinase
	Nucleotide binding	202 – 210	9	ATP By similarity
	Compositional bias	4 – 146	143	Ser-rich
	Compositional bias	567 – 624	58	Pro-rich
Sites
	Active site	329	1	Proton acceptor By similarity
	Binding site	226	1	ATP By similarity
Experimental info
	Sequence conflict	241 – 244	4	KRCS → NAAP in CAA82043. Ref.1
	Sequence conflict	300	1	S → H in CAA82043. Ref.1
	Sequence conflict	356 – 375	20	TDPHD…MIGSP → HGSTRPVQPCQEVRRDDRLA in CAA82043. Ref.1
	Sequence conflict	637 – 662	26	VHHHL…VPAST → GASPSEHCQQLGP in CAA82042. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	P36002 [UniParc]. Last modified September 21, 2011. Version 3. Checksum: 78952B1828D47048	FASTA	662	73,502
	10 20 30 40 50 60 MTVSHNHSTK ISQQPISSVS AFKFFGKKLL SSSHGNKLKK KASLPPDFHS TSTNDSESSS 70 80 90 100 110 120 PKLPNSLKTS RRANSFAHTT NSKRSLSSAS TKILPPAGSS TSISRGNRHS STSRNLSNSK 130 140 150 160 170 180 FSSERLVYNP YGVSTPSTSL SSVSTSMKKD PDLGFYLHDG DSKIRMLPIP IVDPNEYLPD 190 200 210 220 230 240 EMKEASIQLS DNFVFDDENK TIGWGGSCEV RKIRSKYRKK DVFALKKLNM IYNETPEKFY 250 260 270 280 290 300 KRCSKEFIIA KQLSHHVHIT NTFLLVKVPT TVYTTRGWGF VMELGLRDLF AMIQKSGWRS 310 320 330 340 350 360 VALAEKFCIF KQVACGVKFC HDQGIAHRDL KPENVLLSPD GVCKLTDFGI SDWYHTDPHD 370 380 390 400 410 420 LSSPVKKCAG MIGSPPYAPP EVMFYDSKKH YDTELQQPYD PRALDCYGLG IILMTLVNNV 430 440 450 460 470 480 IPFLESCSFD TGFRDYCDAY ENFIRLHDRA FRNRGNYRPG PGMEYHLARN FKNGHASRVA 490 500 510 520 530 540 WRLADPEAAT RYTIDDLFED PWFQGIETCV DANDKYVCKK PIIKTTTYEN PRGFHIATDV 550 560 570 580 590 600 AATTPTSNPF LKNRVPIRSM VDIAAHPSPT ATVLASSPPP PPPATHVPAE ALFTLRETPP 610 620 630 640 650 660 PQLATLTLSE EPPATPAPSA PSAPSARVRG HSPHRVVHHH LNIVNSLVHS SSAASSQVPA ST

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Complete DNA sequence of yeast chromosome XI." Dujon B., Alexandraki D., Andre B., Ansorge W., Baladron V., Ballesta J.P.G., Banrevi A., Bolle P.-A., Bolotin-Fukuhara M., Bossier P., Bou G., Boyer J., Buitrago M.J., Cheret G., Colleaux L., Daignan-Fornier B., del Rey F., Dion C. , Domdey H., Duesterhoeft A., Duesterhus S., Entian K.-D., Erfle H., Esteban P.F., Feldmann H., Fernandes L., Fobo G.M., Fritz C., Fukuhara H., Gabel C., Gaillon L., Garcia-Cantalejo J.M., Garcia-Ramirez J.J., Gent M.E., Ghazvini M., Goffeau A., Gonzalez A., Grothues D., Guerreiro P., Hegemann J.H., Hewitt N., Hilger F., Hollenberg C.P., Horaitis O., Indge K.J., Jacquier A., James C.M., Jauniaux J.-C., Jimenez A., Keuchel H., Kirchrath L., Kleine K., Koetter P., Legrain P., Liebl S., Louis E.J., Maia e Silva A., Marck C., Monnier A.-L., Moestl D., Mueller S., Obermaier B., Oliver S.G., Pallier C., Pascolo S., Pfeiffer F., Philippsen P., Planta R.J., Pohl F.M., Pohl T.M., Poehlmann R., Portetelle D., Purnelle B., Puzos V., Ramezani Rad M., Rasmussen S.W., Remacha M.A., Revuelta J.L., Richard G.-F., Rieger M., Rodrigues-Pousada C., Rose M., Rupp T., Santos M.A., Schwager C., Sensen C., Skala J., Soares H., Sor F., Stegemann J., Tettelin H., Thierry A., Tzermia M., Urrestarazu L.A., van Dyck L., van Vliet-Reedijk J.C., Valens M., Vandenbol M., Vilela C., Vissers S., von Wettstein D., Voss H., Wiemann S., Xu G., Zimmermann J., Haasemann M., Becker I., Mewes H.-W. Nature 369:371-378(1994) [PubMed: 8196765] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 96604 / S288c / FY1679.
[2]	Saccharomyces Genome Database Submitted (MAR-2011) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION, SEQUENCE REVISION TO 241-244; 300; 356-375 AND 637-662. Strain: ATCC 204508 / S288c.
[3]	"Cloning of the gene encoding a putative serine/threonine protein kinase which enhances spermine uptake in Saccharomyces cerevisiae." Kakinuma Y., Maruyama T., Nozaki T., Wada Y., Ohsumi Y., Igarashi K. Biochem. Biophys. Res. Commun. 216:985-992(1995) [PubMed: 7488221] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-584, CHARACTERIZATION.
[4]	"The STK2 gene, which encodes a putative Ser/Thr protein kinase, is required for high-affinity spermidine transport in Saccharomyces cerevisiae." Kaouass M., Audette M., Ramotar D., Verma S., de Montigny D., Gamache I., Torossian K., Poulin R. Mol. Cell. Biol. 17:2994-3004(1997) [PubMed: 9154797] [Abstract] Cited for: CHARACTERIZATION.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	Z28198 Genomic DNA. Translation: CAA82043.1. Frameshift. Z28198 Genomic DNA. Translation: CAA82042.1. Frameshift. D63815 Genomic DNA. Translation: BAA09884.1. Frameshift. BK006944 Genomic DNA. Translation: DAA08968.2.
PIR	S38035. S38036.
RefSeq	NP_012723.3. NM_001179764.2.
3D structure databases
ProteinModelPortal	P36002.
SMR	P36002. Positions 197-508.
ModBase	Search...
Protein-protein interaction databases
IntAct	P36002. 14 interactions.
MINT	MINT-6490143.
STRING	P36002.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	853635.
KEGG	sce:YKL198C.
NMPDR	fig|4932.3.peg.3700.
Organism-specific databases
CYGD	YKL198c.
SGD	S000001681. PTK1.
Phylogenomic databases
eggNOG	fuNOG04254.
GeneTree	EFGT00070000008718.
HOGENOM	HBG202364.
OMA	ACIRNIN.
OrthoDB	EOG4RJK8Z.
Enzyme and pathway databases
BRENDA	2.7.11.1. 984.
Gene expression databases
ArrayExpress	P36002.
Genevestigator	P36002.
GermOnline	YKL198C. Saccharomyces cerevisiae.
Family and domain databases
InterPro	IPR011009. Kinase-like_dom. IPR000719. Prot_kinase_cat_dom. IPR017442. Se/Thr_kinase-like_dom. IPR008271. Ser/Thr_kinase_AS. [Graphical view]
KO	K08286.
Pfam	PF00069. Pkinase. 1 hit. [Graphical view]
SUPFAM	SSF56112. Kinase_like. 1 hit.
PROSITE	PS00107. PROTEIN_KINASE_ATP. False negative. PS50011. PROTEIN_KINASE_DOM. 1 hit. PS00108. PROTEIN_KINASE_ST. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	974517.

Entry information

Entry name

PTK1_YEAST

Accession

Primary (citable) accession number: P36002
Secondary accession number(s): D6VX02, P36045, Q06941

Entry history

Integrated into UniProtKB/Swiss-Prot:	June 1, 1994
Last sequence update:	September 21, 2011
Last modified:	January 25, 2012
This is version 103 of the entry and version 3 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program