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Protein

Probable folylpolyglutamate synthase

Gene

RMA1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Protein inferred from homologyi

Functioni

Conversion of folates to polyglutamate derivatives.

Catalytic activityi

ATP + tetrahydropteroyl-(gamma-Glu)(n) + L-glutamate = ADP + phosphate + tetrahydropteroyl-(gamma-Glu)(n+1).

Pathwayi: tetrahydrofolylpolyglutamate biosynthesis

This protein is involved in the pathway tetrahydrofolylpolyglutamate biosynthesis, which is part of Cofactor biosynthesis.
View all proteins of this organism that are known to be involved in the pathway tetrahydrofolylpolyglutamate biosynthesis and in Cofactor biosynthesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi132Magnesium 1By similarity1
Binding sitei300ATPBy similarity1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi37 – 40ATPBy similarity4

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionLigase
Biological processOne-carbon metabolism
LigandATP-binding, Magnesium, Metal-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciYEAST:YKL132C-MONOMER.
UniPathwayiUPA00850.

Names & Taxonomyi

Protein namesi
Recommended name:
Probable folylpolyglutamate synthase (EC:6.3.2.17)
Alternative name(s):
Folylpoly-gamma-glutamate synthetase
Short name:
FPGS
Tetrahydrofolylpolyglutamate synthase
Short name:
Tetrahydrofolate synthase
Gene namesi
Name:RMA1
Ordered Locus Names:YKL132C
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome XI

Organism-specific databases

EuPathDBiFungiDB:YKL132C.
SGDiS000001615. RMA1.

Subcellular locationi

GO - Cellular componenti

Keywords - Cellular componenti

Mitochondrion

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001683071 – 430Probable folylpolyglutamate synthaseAdd BLAST430

Proteomic databases

PRIDEiP36001.

Interactioni

Protein-protein interaction databases

BioGridi34004. 81 interactors.
IntActiP36001. 1 interactor.
MINTiMINT-2780244.
STRINGi4932.YKL132C.

Structurei

3D structure databases

ProteinModelPortaliP36001.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the folylpolyglutamate synthase family.Curated

Phylogenomic databases

GeneTreeiENSGT00550000075841.
HOGENOMiHOG000019981.
InParanoidiP36001.
KOiK20457.
OMAiLTYFEMG.
OrthoDBiEOG092C0KWV.

Family and domain databases

Gene3Di3.40.1190.10. 1 hit.
InterProiView protein in InterPro
IPR001645. Folylpolyglutamate_synth.
IPR018109. Folylpolyglutamate_synth_CS.
IPR004101. Mur_ligase_C.
IPR013221. Mur_ligase_cen.
PANTHERiPTHR11136. PTHR11136. 1 hit.
SUPFAMiSSF53244. SSF53244. 1 hit.
SSF53623. SSF53623. 1 hit.
TIGRFAMsiTIGR01499. folC. 1 hit.
PROSITEiView protein in PROSITE
PS01011. FOLYLPOLYGLU_SYNT_1. 1 hit.
PS01012. FOLYLPOLYGLU_SYNT_2. 1 hit.

Sequencei

Sequence statusi: Complete.

P36001-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MDDISGRQTL PRINRLLEHV GNPQDSLSIL HIAGTNGKET VSKFLTSILQ
60 70 80 90 100
HPGQQRQRVL IGRYTTSSLL NAKEEDISIN NEAISLIEYS RIEKELIEAD
110 120 130 140 150
SSLKLQCNNL ELLTSVALVY FAKKNCQWCI IETGLAGKQD PGSIIAGQSR
160 170 180 190 200
VCCAITNVGI SDEAFLCKFL SQITESSTNK AIFLLDGSND EFVRNTITKR
210 220 230 240 250
CHDVGCPLEI TDPSLRDYNV HTDTWGTLEV RLPYSEEEYQ IFNLRVAIAV
260 270 280 290 300
LDFLSKEKKV CISKDQLSQG LISVDWPRSL HRLDYCYEST SGKKIALLLD
310 320 330 340 350
NANNAKAARN LACHLRTTYG DTPLTFVIAI TTGKKVSPLL DPLIRPQDYV
360 370 380 390 400
IVTRFGSVVG MPWIQSLEPV NLLAFIKNRY TRNVNMQPDL QSVWTFLETS
410 420 430
GLKTIVPVIV CGSLYICKEL LRLHNCHLPV
Length:430
Mass (Da):48,143
Last modified:June 1, 1994 - v1
Checksum:iD7CC48A41044FCA0
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z28131 Genomic DNA. Translation: CAA81972.1.
BK006944 Genomic DNA. Translation: DAA09030.1.
PIRiS37961.
RefSeqiNP_012790.1. NM_001179698.1.

Genome annotation databases

EnsemblFungiiYKL132C; YKL132C; YKL132C.
GeneIDi853727.
KEGGisce:YKL132C.

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.

Entry informationi

Entry nameiFOLC_YEAST
AccessioniPrimary (citable) accession number: P36001
Secondary accession number(s): D6VX64
Entry historyiIntegrated into UniProtKB/Swiss-Prot: June 1, 1994
Last sequence update: June 1, 1994
Last modified: July 5, 2017
This is version 136 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families
  3. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  4. Yeast chromosome XI
    Yeast (Saccharomyces cerevisiae) chromosome XI: entries and gene names