Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Probable folylpolyglutamate synthase

Gene

RMA1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Conversion of folates to polyglutamate derivatives.

Catalytic activityi

ATP + tetrahydropteroyl-(gamma-Glu)(n) + L-glutamate = ADP + phosphate + tetrahydropteroyl-(gamma-Glu)(n+1).

Pathwayi: tetrahydrofolylpolyglutamate biosynthesis

This protein is involved in the pathway tetrahydrofolylpolyglutamate biosynthesis, which is part of Cofactor biosynthesis.
View all proteins of this organism that are known to be involved in the pathway tetrahydrofolylpolyglutamate biosynthesis and in Cofactor biosynthesis.

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi33 – 397ATPSequence analysis

GO - Molecular functioni

  • ATP binding Source: UniProtKB-KW
  • tetrahydrofolylpolyglutamate synthase activity Source: SGD

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Ligase

Keywords - Biological processi

One-carbon metabolism

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciYEAST:YKL132C-MONOMER.
UniPathwayiUPA00850.

Names & Taxonomyi

Protein namesi
Recommended name:
Probable folylpolyglutamate synthase (EC:6.3.2.17)
Alternative name(s):
Folylpoly-gamma-glutamate synthetase
Short name:
FPGS
Tetrahydrofolylpolyglutamate synthase
Short name:
Tetrahydrofolate synthase
Gene namesi
Name:RMA1
Ordered Locus Names:YKL132C
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome XI

Organism-specific databases

EuPathDBiFungiDB:YKL132C.
SGDiS000001615. RMA1.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Mitochondrion

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 430430Probable folylpolyglutamate synthasePRO_0000168307Add
BLAST

Interactioni

Protein-protein interaction databases

BioGridi34004. 65 interactions.
IntActiP36001. 1 interaction.
MINTiMINT-2780244.

Structurei

3D structure databases

ProteinModelPortaliP36001.
SMRiP36001. Positions 7-343.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the folylpolyglutamate synthase family.Curated

Phylogenomic databases

GeneTreeiENSGT00550000075841.
HOGENOMiHOG000019981.
InParanoidiP36001.
KOiK01930.
OrthoDBiEOG7NKKX2.

Family and domain databases

Gene3Di3.40.1190.10. 1 hit.
3.90.190.20. 1 hit.
InterProiIPR001645. Folylpolyglutamate_synth.
IPR018109. Folylpolyglutamate_synth_CS.
IPR004101. Mur_ligase_C.
IPR013221. Mur_ligase_cen.
[Graphical view]
PANTHERiPTHR11136. PTHR11136. 1 hit.
SUPFAMiSSF53244. SSF53244. 1 hit.
SSF53623. SSF53623. 1 hit.
TIGRFAMsiTIGR01499. folC. 1 hit.
PROSITEiPS01011. FOLYLPOLYGLU_SYNT_1. 1 hit.
PS01012. FOLYLPOLYGLU_SYNT_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P36001-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MDDISGRQTL PRINRLLEHV GNPQDSLSIL HIAGTNGKET VSKFLTSILQ
60 70 80 90 100
HPGQQRQRVL IGRYTTSSLL NAKEEDISIN NEAISLIEYS RIEKELIEAD
110 120 130 140 150
SSLKLQCNNL ELLTSVALVY FAKKNCQWCI IETGLAGKQD PGSIIAGQSR
160 170 180 190 200
VCCAITNVGI SDEAFLCKFL SQITESSTNK AIFLLDGSND EFVRNTITKR
210 220 230 240 250
CHDVGCPLEI TDPSLRDYNV HTDTWGTLEV RLPYSEEEYQ IFNLRVAIAV
260 270 280 290 300
LDFLSKEKKV CISKDQLSQG LISVDWPRSL HRLDYCYEST SGKKIALLLD
310 320 330 340 350
NANNAKAARN LACHLRTTYG DTPLTFVIAI TTGKKVSPLL DPLIRPQDYV
360 370 380 390 400
IVTRFGSVVG MPWIQSLEPV NLLAFIKNRY TRNVNMQPDL QSVWTFLETS
410 420 430
GLKTIVPVIV CGSLYICKEL LRLHNCHLPV
Length:430
Mass (Da):48,143
Last modified:June 1, 1994 - v1
Checksum:iD7CC48A41044FCA0
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z28131 Genomic DNA. Translation: CAA81972.1.
BK006944 Genomic DNA. Translation: DAA09030.1.
PIRiS37961.
RefSeqiNP_012790.1. NM_001179698.1.

Genome annotation databases

EnsemblFungiiYKL132C; YKL132C; YKL132C.
GeneIDi853727.
KEGGisce:YKL132C.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z28131 Genomic DNA. Translation: CAA81972.1.
BK006944 Genomic DNA. Translation: DAA09030.1.
PIRiS37961.
RefSeqiNP_012790.1. NM_001179698.1.

3D structure databases

ProteinModelPortaliP36001.
SMRiP36001. Positions 7-343.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi34004. 65 interactions.
IntActiP36001. 1 interaction.
MINTiMINT-2780244.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYKL132C; YKL132C; YKL132C.
GeneIDi853727.
KEGGisce:YKL132C.

Organism-specific databases

EuPathDBiFungiDB:YKL132C.
SGDiS000001615. RMA1.

Phylogenomic databases

GeneTreeiENSGT00550000075841.
HOGENOMiHOG000019981.
InParanoidiP36001.
KOiK01930.
OrthoDBiEOG7NKKX2.

Enzyme and pathway databases

UniPathwayiUPA00850.
BioCyciYEAST:YKL132C-MONOMER.

Miscellaneous databases

NextBioi974758.
PROiP36001.

Family and domain databases

Gene3Di3.40.1190.10. 1 hit.
3.90.190.20. 1 hit.
InterProiIPR001645. Folylpolyglutamate_synth.
IPR018109. Folylpolyglutamate_synth_CS.
IPR004101. Mur_ligase_C.
IPR013221. Mur_ligase_cen.
[Graphical view]
PANTHERiPTHR11136. PTHR11136. 1 hit.
SUPFAMiSSF53244. SSF53244. 1 hit.
SSF53623. SSF53623. 1 hit.
TIGRFAMsiTIGR01499. folC. 1 hit.
PROSITEiPS01011. FOLYLPOLYGLU_SYNT_1. 1 hit.
PS01012. FOLYLPOLYGLU_SYNT_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Complete DNA sequence of yeast chromosome XI."
    Dujon B., Alexandraki D., Andre B., Ansorge W., Baladron V., Ballesta J.P.G., Banrevi A., Bolle P.-A., Bolotin-Fukuhara M., Bossier P., Bou G., Boyer J., Buitrago M.J., Cheret G., Colleaux L., Daignan-Fornier B., del Rey F., Dion C.
    , Domdey H., Duesterhoeft A., Duesterhus S., Entian K.-D., Erfle H., Esteban P.F., Feldmann H., Fernandes L., Fobo G.M., Fritz C., Fukuhara H., Gabel C., Gaillon L., Garcia-Cantalejo J.M., Garcia-Ramirez J.J., Gent M.E., Ghazvini M., Goffeau A., Gonzalez A., Grothues D., Guerreiro P., Hegemann J.H., Hewitt N., Hilger F., Hollenberg C.P., Horaitis O., Indge K.J., Jacquier A., James C.M., Jauniaux J.-C., Jimenez A., Keuchel H., Kirchrath L., Kleine K., Koetter P., Legrain P., Liebl S., Louis E.J., Maia e Silva A., Marck C., Monnier A.-L., Moestl D., Mueller S., Obermaier B., Oliver S.G., Pallier C., Pascolo S., Pfeiffer F., Philippsen P., Planta R.J., Pohl F.M., Pohl T.M., Poehlmann R., Portetelle D., Purnelle B., Puzos V., Ramezani Rad M., Rasmussen S.W., Remacha M.A., Revuelta J.L., Richard G.-F., Rieger M., Rodrigues-Pousada C., Rose M., Rupp T., Santos M.A., Schwager C., Sensen C., Skala J., Soares H., Sor F., Stegemann J., Tettelin H., Thierry A., Tzermia M., Urrestarazu L.A., van Dyck L., van Vliet-Reedijk J.C., Valens M., Vandenbol M., Vilela C., Vissers S., von Wettstein D., Voss H., Wiemann S., Xu G., Zimmermann J., Haasemann M., Becker I., Mewes H.-W.
    Nature 369:371-378(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  2. Cited for: GENOME REANNOTATION.
    Strain: ATCC 204508 / S288c.
  3. Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
    Strain: ATCC 76625 / YPH499.

Entry informationi

Entry nameiFOLC_YEAST
AccessioniPrimary (citable) accession number: P36001
Secondary accession number(s): D6VX64
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 1, 1994
Last sequence update: June 1, 1994
Last modified: May 11, 2016
This is version 126 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families
  3. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  4. Yeast chromosome XI
    Yeast (Saccharomyces cerevisiae) chromosome XI: entries and gene names

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.