P36000 (AP1B1_YEAST) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
AP-1 complex subunit beta-1

Alternative name(s):
Beta-1-adaptin
Clathrin assembly protein complex 1 beta-1 large chain
Clathrin assembly protein large beta-1 chain

Gene names

Name:	APL2
Ordered Locus Names:	YKL135C

Organism

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)

Taxonomic identifier

559292 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces

Protein attributes

Sequence length	726 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Adaptins are components of the adaptor complexes which link clathrin to receptors in coated vesicles. Clathrin-associated protein complexes are believed to interact with the cytoplasmic tails of membrane proteins, leading to their selection and concentration. The AP-1 complex interacts directly with clathrin. Ref.4
Subunit structure	Assembly protein complex 1 (AP-1) is a heterotetramer composed of two large adaptins (gamma-type subunit APL4 and beta-type subunit APL2), a medium adaptin (mu-type subunit APM1) and a small adaptin (sigma-type subunit APS1). Interacts with CHC1. Interacts with APM2, probably forming an alternative AP-1-like complex. Ref.4
Subcellular location	Cell membrane. Membrane › coated pit; Peripheral membrane protein; Cytoplasmic side. Note: Component of the coat surrounding the cytoplasmic face of coated vesicles in the plasma membrane.
Miscellaneous	Present with 2690 molecules/cell in log phase SD medium. Ref.5
Sequence similarities	Belongs to the adaptor complexes large subunit family.

Ontologies

Keywords
Cellular component	Cell membrane Coated pit Membrane
PTM	Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	Golgi to vacuole transport Inferred from mutant phenotype. Source: SGD intracellular protein transport Inferred from electronic annotation. Source: InterPro
Cellular component	AP-1 adaptor complex Inferred from physical interaction Ref.4. Source: SGD coated pit Inferred from electronic annotation. Source: UniProtKB-SubCell plasma membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	clathrin binding Inferred from physical interaction Ref.4. Source: SGD protein transporter activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
APL4	Q12028	6	EBI-2206,EBI-33025
APM1	Q00776	9	EBI-2206,EBI-2624
APM2	P38700	7	EBI-2206,EBI-2705
APS1	P35181	5	EBI-2206,EBI-2612
CHC1	P22137	3	EBI-2206,EBI-4766

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 726	726	AP-1 complex subunit beta-1		PRO_0000193753
Amino acid modifications
	Modified residue	286	1	Phosphotyrosine Ref.6
	Modified residue	287	1	Phosphoserine Ref.6
	Modified residue	290	1	Phosphoserine Ref.6

Sequences

	Sequence		Length	Mass (Da)
	P36000 [UniParc]. Last modified June 1, 1994. Version 1. Checksum: 89893945D4EC6C4A	FASTA	726	81,869
	10 20 30 40 50 60 MPPLDKRIKK FLKDSIRIAP KISGKGELSE LRTGLVSQYP QTRKDAIKKT IQQMTLGKDV 70 80 90 100 110 120 SSLFPDVLKN IATIDVEQKK LVYLYVMNYA ETHPELCILA VNTFITDAQD PNPLIRCMAI 130 140 150 160 170 180 RTMSMIRVDK ILEYIETPLR RTLHDDNAYV RKTAVICVAK LFQLNKDLCV ELGVVEDLVN 190 200 210 220 230 240 ALDDSNPLVI ANATAALIEI HNMDMDAVDL SSLIQSHVSQ FLLALNECTE WARIIILGTL 250 260 270 280 290 300 SEYSAKDSLE AQDIIDRVTA HLQHVNPAVV LATIKVIVRN LPQIEYSSNS LIMKRLSSAF 310 320 330 340 350 360 VSLMSTPPEM QYVALKNIRI ILEKYPELLT KELRIFYVKF NDPLYVKLEK IDILVRLVDP 370 380 390 400 410 420 SNLKQCTLLL TELKEYAMEY EPEFVSRAIQ ALSQLGIKYA QESFVSKVLD ILLELLERQD 430 440 450 460 470 480 TIKDDCCISL CDLLRHCPGN DKMAKQVCAV FNTWSNPEVL LQSDIAKCNY VWLLGQHPNN 490 500 510 520 530 540 FSDLESKINI FIENFVQEEA LTQMSLLMTI VRLHATLTGS MLQSVLELAT QQTHELDVRD 550 560 570 580 590 600 MAMMYWRCLS MPNNESLVND LCQNKLPMIS NTLEKFSPEV LEKLLMELGT ISSIYFKPDS 610 620 630 640 650 660 NRRKGKKYVQ NIVKGKHIEE LESMAKNEIS SKANDDVLLD FDERDDVTNT NAGMLNTLTT 670 680 690 700 710 720 LGDLDDLFDF GPSEDATQIN TNDTKAVQGL KELKLGGDSN GISSGGKNNP DVSGGNIVSQ DLLDLF

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Saccharomyces cerevisiae Apl2p, a homologue of the mammalian clathrin AP beta subunit, plays a role in clathrin-dependent Golgi functions." Rad M.R., Phan H.L., Kirchrath L., Tan P.K., Kirchhausen T., Hollenberg C.P., Payne G.S. J. Cell Sci. 108:1605-1615(1995) [PubMed: 7615679] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"Complete DNA sequence of yeast chromosome XI." Dujon B., Alexandraki D., Andre B., Ansorge W., Baladron V., Ballesta J.P.G., Banrevi A., Bolle P.-A., Bolotin-Fukuhara M., Bossier P., Bou G., Boyer J., Buitrago M.J., Cheret G., Colleaux L., Daignan-Fornier B., del Rey F., Dion C. , Domdey H., Duesterhoeft A., Duesterhus S., Entian K.-D., Erfle H., Esteban P.F., Feldmann H., Fernandes L., Fobo G.M., Fritz C., Fukuhara H., Gabel C., Gaillon L., Garcia-Cantalejo J.M., Garcia-Ramirez J.J., Gent M.E., Ghazvini M., Goffeau A., Gonzalez A., Grothues D., Guerreiro P., Hegemann J.H., Hewitt N., Hilger F., Hollenberg C.P., Horaitis O., Indge K.J., Jacquier A., James C.M., Jauniaux J.-C., Jimenez A., Keuchel H., Kirchrath L., Kleine K., Koetter P., Legrain P., Liebl S., Louis E.J., Maia e Silva A., Marck C., Monnier A.-L., Moestl D., Mueller S., Obermaier B., Oliver S.G., Pallier C., Pascolo S., Pfeiffer F., Philippsen P., Planta R.J., Pohl F.M., Pohl T.M., Poehlmann R., Portetelle D., Purnelle B., Puzos V., Ramezani Rad M., Rasmussen S.W., Remacha M.A., Revuelta J.L., Richard G.-F., Rieger M., Rodrigues-Pousada C., Rose M., Rupp T., Santos M.A., Schwager C., Sensen C., Skala J., Soares H., Sor F., Stegemann J., Tettelin H., Thierry A., Tzermia M., Urrestarazu L.A., van Dyck L., van Vliet-Reedijk J.C., Valens M., Vandenbol M., Vilela C., Vissers S., von Wettstein D., Voss H., Wiemann S., Xu G., Zimmermann J., Haasemann M., Becker I., Mewes H.-W. Nature 369:371-378(1994) [PubMed: 8196765] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 96604 / S288c / FY1679.
[3]	Saccharomyces Genome Database Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: ATCC 204508 / S288c.
[4]	"Adaptor complex-independent clathrin function in yeast." Yeung B.G., Phan H.L., Payne G.S. Mol. Biol. Cell 10:3643-3659(1999) [PubMed: 10564262] [Abstract] Cited for: FUNCTION, SUBUNIT, INTERACTION WITH APM2 AND CHC1.
[5]	"Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed: 14562106] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[6]	"A multidimensional chromatography technology for in-depth phosphoproteome analysis." Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H. Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed: 18407956] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT TYR-286; SER-287 AND SER-290, MASS SPECTROMETRY.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	Z30212 Genomic DNA. Translation: CAA82931.1. Z28135 Genomic DNA. Translation: CAA81977.1. BK006944 Genomic DNA. Translation: DAA09027.1.
PIR	S37964.
RefSeq	NP_012787.1. NM_001179701.1.
3D structure databases
ProteinModelPortal	P36000.
SMR	P36000. Positions 22-603.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-1199N.
IntAct	P36000. 46 interactions.
MINT	MINT-404361.
STRING	P36000.
Proteomic databases
PeptideAtlas	P36000.
PRIDE	P36000.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblFungi	YKL135C; YKL135C; YKL135C.
GeneID	853723.
KEGG	sce:YKL135C.
NMPDR	fig|4932.3.peg.3768.
Organism-specific databases
CYGD	YKL135c.
SGD	S000001618. APL2.
Phylogenomic databases
eggNOG	fuNOG06950.
GeneTree	EFGT00050000000022.
HOGENOM	HBG718694.
OMA	ENPYVRK.
OrthoDB	EOG49GPQW.
Gene expression databases
ArrayExpress	P36000.
Genevestigator	P36000.
GermOnline	YKL135C. Saccharomyces cerevisiae.
Family and domain databases
InterPro	IPR016342. AP_complex_bsu. IPR011989. ARM-like. IPR016024. ARM-type_fold. IPR002553. Clathrin/coatomer_adapt-like_N. [Graphical view]
Gene3D	G3DSA:1.25.10.10. ARM-like. 1 hit.
Pfam	PF01602. Adaptin_N. 1 hit. [Graphical view]
PIRSF	PIRSF002291. AP_complex_beta. 1 hit.
SUPFAM	SSF48371. ARM-type_fold. 1 hit.
ProtoNet	Search...
Other
NextBio	974749.

Entry information

Entry name

AP1B1_YEAST

Accession

Primary (citable) accession number: P36000
Secondary accession number(s): D6VX61

Entry history

Integrated into UniProtKB/Swiss-Prot:	June 1, 1994
Last sequence update:	June 1, 1994
Last modified:	December 14, 2011
This is version 92 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program