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Protein

Mitochondrial intermediate peptidase

Gene

OCT1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Cleaves proteins, imported into the mitochondrion, to their mature size. While most mitochondrial precursor proteins are processed to the mature form in one step by mitochondrial processing peptidase (MPP), the sequential cleavage by MIP of an octapeptide after initial processing by MPP is a required step for a subgroup of nuclear-encoded precursor proteins destined for the matrix or the inner membrane. Cleaves precursor proteins of respiratory components, including subunits of the electron transport chain and tricarboxylic acid cycle enzymes, and components of the mitochondrial genetic machinery, including ribosomal proteins, translation factors, and proteins required for mitochondrial DNA metabolism.2 Publications

Catalytic activityi

Release of an N-terminal octapeptide as second stage of processing of some proteins imported into the mitochondrion.

Cofactori

Zn2+By similarityNote: Binds 1 zinc ion.By similarity

Enzyme regulationi

Stimulated by Fe2+.1 Publication

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi558Zinc; catalyticPROSITE-ProRule annotation1
Active sitei559PROSITE-ProRule annotation1
Metal bindingi562Zinc; catalyticPROSITE-ProRule annotation1
Metal bindingi565Zinc; catalyticPROSITE-ProRule annotation1

GO - Molecular functioni

  • metal ion binding Source: UniProtKB-KW
  • metalloendopeptidase activity Source: SGD

GO - Biological processi

  • cellular iron ion homeostasis Source: SGD
  • peptide metabolic process Source: GO_Central
  • protein processing involved in protein targeting to mitochondrion Source: SGD
  • protein stabilization Source: SGD
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Metalloprotease, Protease

Keywords - Ligandi

Metal-binding, Zinc

Enzyme and pathway databases

BioCyciYEAST:YKL134C-MONOMER.

Protein family/group databases

MEROPSiM03.006.

Names & Taxonomyi

Protein namesi
Recommended name:
Mitochondrial intermediate peptidase (EC:3.4.24.59)
Short name:
MIP
Gene namesi
Name:OCT1
Synonyms:MIP1
Ordered Locus Names:YKL134C
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome XI

Organism-specific databases

EuPathDBiFungiDB:YKL134C.
SGDiS000001617. OCT1.

Subcellular locationi

GO - Cellular componenti

  • mitochondrial matrix Source: SGD
Complete GO annotation...

Keywords - Cellular componenti

Mitochondrion

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi131C → S or V: Affects protein stability, but has no effect on peptidase activity. 1 Publication1
Mutagenesisi557F → R: Affects protein stability. 1 Publication1
Mutagenesisi558H → R: Abolishes proteolytic activity. 1 Publication1
Mutagenesisi559E → D: Abolishes proteolytic activity. 1 Publication1
Mutagenesisi561G → L: Affects protein stability. 1 Publication1
Mutagenesisi562H → R: Abolishes proteolytic activity. 1 Publication1
Mutagenesisi565H → R: Temperature sensitive; abolishes proteolytic activity for RIP1, but not for COX4. 1 Publication1
Mutagenesisi578G → L: Temperature sensitive; abolishes proteolytic activity for RIP1, but not for COX4. 1 Publication1
Mutagenesisi581C → S or V: Affects protein stability, but has no effect on peptidase activity. 1 Publication1
Mutagenesisi584D → E: No effect. 1 Publication1
Mutagenesisi587E → D: Abolishes proteolytic activity. 1 Publication1
Mutagenesisi589P → L: No effect. 1 Publication1
Mutagenesisi590S → Y: Affects protein stability. 1 Publication1
Mutagenesisi594E → D: No effect. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transit peptidei1 – 37MitochondrionSequence analysisAdd BLAST37
ChainiPRO_000002858338 – 772Mitochondrial intermediate peptidaseAdd BLAST735

Proteomic databases

MaxQBiP35999.
PRIDEiP35999.

Interactioni

Protein-protein interaction databases

BioGridi34002. 62 interactors.
DIPiDIP-2670N.
IntActiP35999. 1 interactor.
MINTiMINT-497669.

Structurei

3D structure databases

ProteinModelPortaliP35999.
SMRiP35999.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the peptidase M3 family.Curated

Keywords - Domaini

Transit peptide

Phylogenomic databases

GeneTreeiENSGT00550000075047.
HOGENOMiHOG000076521.
InParanoidiP35999.
KOiK01410.
OMAiIIIDGLH.
OrthoDBiEOG092C0JU2.

Family and domain databases

CDDicd06457. M3A_MIP. 1 hit.
Gene3Di1.10.1370.10. 2 hits.
1.20.1050.40. 1 hit.
3.40.390.10. 1 hit.
InterProiIPR033851. M3A_MIP.
IPR024079. MetalloPept_cat_dom.
IPR024077. Neurolysin/TOP_dom2.
IPR024080. Neurolysin/TOP_N.
IPR001567. Pept_M3A_M3B.
[Graphical view]
PANTHERiPTHR11804:SF5. PTHR11804:SF5. 2 hits.
PfamiPF01432. Peptidase_M3. 1 hit.
[Graphical view]
PROSITEiPS00142. ZINC_PROTEASE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P35999-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MLRTIILKAG SNASIPSPSR QNKLLRFFAT AGAVSRTSPG SIKKIFDDNS
60 70 80 90 100
YWRNINGQDA NNSKISQYLF KKNKTGLFKN PYLTSPDGLR KFSQVSLQQA
110 120 130 140 150
QELLDKMRND FSESGKLTYI MNLDRLSDTL CRVIDLCEFI RSTHPDDAFV
160 170 180 190 200
RAAQDCHEQM FEFMNVLNTD VSLCNILKSV LNNPEVSSKL SAEELKVGKI
210 220 230 240 250
LLDDFEKSGI YMNPDVREKF IQLSQEISLV GQEFINHTDY PGSNSVKIPC
260 270 280 290 300
KDLDNSKVST FLLKQLNKDV KGQNYKVPTF GYAAYALLKS CENEMVRKKL
310 320 330 340 350
WTALHSCSDK QVKRLSHLIK LRAILANLMH KTSYAEYQLE GKMAKNPKDV
360 370 380 390 400
QDFILTLMNN TIEKTANELK FIAELKAKDL KKPLTTNTDE ILKLVRPWDR
410 420 430 440 450
DYYTGKYFQL NPSNSPNAKE ISYYFTLGNV IQGLSDLFQQ IYGIRLEPAI
460 470 480 490 500
TDEGETWSPD VRRLNVISEE EGIIGIIYCD LFERNGKTSN PAHFTVCCSR
510 520 530 540 550
QIYPSETDFS TIQVGENPDG TYFQLPVISL VCNFSPILIA SKKSLCFLQL
560 570 580 590 600
SEVETLFHEM GHAMHSMLGR THMQNISGTR CATDFVELPS ILMEHFAKDI
610 620 630 640 650
RILTKIGKHY GTGETIQADM LQRFMKSTNF LQNCETYSQA KMAMLDQSFH
660 670 680 690 700
DEKIISDIDN FDVVENYQAL ERRLKVLVDD QSNWCGRFGH LFGYGATYYS
710 720 730 740 750
YLFDRTIASK IWYALFEDDP YSRKNGDKFK KHLLKWGGLK DPWKCIADVL
760 770
ECPMLEKGGS DAMEFIAQSH KS
Length:772
Mass (Da):88,183
Last modified:November 1, 1997 - v2
Checksum:i1C19A0655FAAE7CA
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti343 – 350MAKNPKDV → WQDRRC in CAA81975 (PubMed:8196765).Curated8
Sequence conflicti694 – 702YGATYYSYL → SGQLITATY in CAA81975 (PubMed:8196765).Curated9

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U10243 Genomic DNA. Translation: AAA21278.1.
Z28134 Genomic DNA. Translation: CAA81975.1.
BK006944 Genomic DNA. Translation: DAA09028.2.
PIRiS37963.
RefSeqiNP_012788.2. NM_001179700.2.

Genome annotation databases

EnsemblFungiiYKL134C; YKL134C; YKL134C.
GeneIDi853724.
KEGGisce:YKL134C.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U10243 Genomic DNA. Translation: AAA21278.1.
Z28134 Genomic DNA. Translation: CAA81975.1.
BK006944 Genomic DNA. Translation: DAA09028.2.
PIRiS37963.
RefSeqiNP_012788.2. NM_001179700.2.

3D structure databases

ProteinModelPortaliP35999.
SMRiP35999.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi34002. 62 interactors.
DIPiDIP-2670N.
IntActiP35999. 1 interactor.
MINTiMINT-497669.

Protein family/group databases

MEROPSiM03.006.

Proteomic databases

MaxQBiP35999.
PRIDEiP35999.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYKL134C; YKL134C; YKL134C.
GeneIDi853724.
KEGGisce:YKL134C.

Organism-specific databases

EuPathDBiFungiDB:YKL134C.
SGDiS000001617. OCT1.

Phylogenomic databases

GeneTreeiENSGT00550000075047.
HOGENOMiHOG000076521.
InParanoidiP35999.
KOiK01410.
OMAiIIIDGLH.
OrthoDBiEOG092C0JU2.

Enzyme and pathway databases

BioCyciYEAST:YKL134C-MONOMER.

Miscellaneous databases

PROiP35999.

Family and domain databases

CDDicd06457. M3A_MIP. 1 hit.
Gene3Di1.10.1370.10. 2 hits.
1.20.1050.40. 1 hit.
3.40.390.10. 1 hit.
InterProiIPR033851. M3A_MIP.
IPR024079. MetalloPept_cat_dom.
IPR024077. Neurolysin/TOP_dom2.
IPR024080. Neurolysin/TOP_N.
IPR001567. Pept_M3A_M3B.
[Graphical view]
PANTHERiPTHR11804:SF5. PTHR11804:SF5. 2 hits.
PfamiPF01432. Peptidase_M3. 1 hit.
[Graphical view]
PROSITEiPS00142. ZINC_PROTEASE. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiPMIP_YEAST
AccessioniPrimary (citable) accession number: P35999
Secondary accession number(s): D6VX62, P51980
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 1, 1994
Last sequence update: November 1, 1997
Last modified: November 30, 2016
This is version 142 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 2690 molecules/cell in log phase SD medium.1 Publication

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Peptidase families
    Classification of peptidase families and list of entries
  2. SIMILARITY comments
    Index of protein domains and families
  3. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  4. Yeast chromosome XI
    Yeast (Saccharomyces cerevisiae) chromosome XI: entries and gene names

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.