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Protein

26S protease regulatory subunit 7

Gene

PSMC2

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Component of the 26S proteasome, a multiprotein complex involved in the ATP-dependent degradation of ubiquitinated proteins. This complex plays a key role in the maintenance of protein homeostasis by removing misfolded or damaged proteins, which could impair cellular functions, and by removing proteins whose functions are no longer required. Therefore, the proteasome participates in numerous cellular processes, including cell cycle progression, apoptosis, or DNA damage repair. PSMC2 belongs to the heterohexameric ring of AAA (ATPases associated with diverse cellular activities) proteins that unfolds ubiquitinated target proteins that are concurrently translocated into a proteolytic chamber and degraded into peptides.2 Publications

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi216 – 223ATPSequence analysis8

GO - Molecular functioni

GO - Biological processi

Keywordsi

Biological processHost-virus interaction
LigandATP-binding, Nucleotide-binding

Enzyme and pathway databases

ReactomeiR-HSA-1169091. Activation of NF-kappaB in B cells.
R-HSA-1234176. Oxygen-dependent proline hydroxylation of Hypoxia-inducible Factor Alpha.
R-HSA-1236974. ER-Phagosome pathway.
R-HSA-1236978. Cross-presentation of soluble exogenous antigens (endosomes).
R-HSA-174084. Autodegradation of Cdh1 by Cdh1:APC/C.
R-HSA-174113. SCF-beta-TrCP mediated degradation of Emi1.
R-HSA-174154. APC/C:Cdc20 mediated degradation of Securin.
R-HSA-174178. APC/C:Cdh1 mediated degradation of Cdc20 and other APC/C:Cdh1 targeted proteins in late mitosis/early G1.
R-HSA-174184. Cdc20:Phospho-APC/C mediated degradation of Cyclin A.
R-HSA-180534. Vpu mediated degradation of CD4.
R-HSA-180585. Vif-mediated degradation of APOBEC3G.
R-HSA-187577. SCF(Skp2)-mediated degradation of p27/p21.
R-HSA-195253. Degradation of beta-catenin by the destruction complex.
R-HSA-202424. Downstream TCR signaling.
R-HSA-211733. Regulation of activated PAK-2p34 by proteasome mediated degradation.
R-HSA-2467813. Separation of Sister Chromatids.
R-HSA-2871837. FCERI mediated NF-kB activation.
R-HSA-349425. Autodegradation of the E3 ubiquitin ligase COP1.
R-HSA-350562. Regulation of ornithine decarboxylase (ODC).
R-HSA-382556. ABC-family proteins mediated transport.
R-HSA-450408. AUF1 (hnRNP D0) binds and destabilizes mRNA.
R-HSA-4608870. Asymmetric localization of PCP proteins.
R-HSA-4641257. Degradation of AXIN.
R-HSA-4641258. Degradation of DVL.
R-HSA-5358346. Hedgehog ligand biogenesis.
R-HSA-5362768. Hh mutants that don't undergo autocatalytic processing are degraded by ERAD.
R-HSA-5607761. Dectin-1 mediated noncanonical NF-kB signaling.
R-HSA-5607764. CLEC7A (Dectin-1) signaling.
R-HSA-5610780. Degradation of GLI1 by the proteasome.
R-HSA-5610783. Degradation of GLI2 by the proteasome.
R-HSA-5610785. GLI3 is processed to GLI3R by the proteasome.
R-HSA-5632684. Hedgehog 'on' state.
R-HSA-5658442. Regulation of RAS by GAPs.
R-HSA-5668541. TNFR2 non-canonical NF-kB pathway.
R-HSA-5676590. NIK-->noncanonical NF-kB signaling.
R-HSA-5678895. Defective CFTR causes cystic fibrosis.
R-HSA-5687128. MAPK6/MAPK4 signaling.
R-HSA-5689603. UCH proteinases.
R-HSA-5689880. Ub-specific processing proteases.
R-HSA-6798695. Neutrophil degranulation.
R-HSA-68827. CDT1 association with the CDC6:ORC:origin complex.
R-HSA-68949. Orc1 removal from chromatin.
R-HSA-69017. CDK-mediated phosphorylation and removal of Cdc6.
R-HSA-69229. Ubiquitin-dependent degradation of Cyclin D1.
R-HSA-69481. G2/M Checkpoints.
R-HSA-69601. Ubiquitin Mediated Degradation of Phosphorylated Cdc25A.
R-HSA-8852276. The role of GTSE1 in G2/M progression after G2 checkpoint.
R-HSA-8854050. FBXL7 down-regulates AURKA during mitotic entry and in early mitosis.
R-HSA-983168. Antigen processing: Ubiquitination & Proteasome degradation.

Names & Taxonomyi

Protein namesi
Recommended name:
26S protease regulatory subunit 7
Alternative name(s):
26S proteasome AAA-ATPase subunit RPT1
Proteasome 26S subunit ATPase 2
Protein MSS1
Gene namesi
Name:PSMC2
Synonyms:MSS1
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 7

Organism-specific databases

HGNCiHGNC:9548. PSMC2.

Subcellular locationi

GO - Cellular componenti

  • cytoplasm Source: UniProtKB
  • cytosol Source: HPA
  • cytosolic proteasome complex Source: GO_Central
  • extracellular region Source: Reactome
  • ficolin-1-rich granule lumen Source: Reactome
  • membrane Source: UniProtKB
  • nuclear proteasome complex Source: GO_Central
  • nucleoplasm Source: Reactome
  • nucleus Source: UniProtKB
  • P-body Source: UniProtKB
  • proteasome accessory complex Source: UniProtKB
  • proteasome complex Source: UniProtKB
  • proteasome regulatory particle, base subcomplex Source: GO_Central
  • secretory granule lumen Source: Reactome

Keywords - Cellular componenti

Cytoplasm, Nucleus, Proteasome

Pathology & Biotechi

Organism-specific databases

DisGeNETi5701.
OpenTargetsiENSG00000161057.
PharmGKBiPA33893.

Polymorphism and mutation databases

BioMutaiPSMC2.
DMDMi547930.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemoved2 Publications
ChainiPRO_00000847092 – 43326S protease regulatory subunit 7Add BLAST432

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei116N6-acetyllysineCombined sources1
Modified residuei422N6-acetyllysineCombined sources1

Keywords - PTMi

Acetylation

Proteomic databases

EPDiP35998.
MaxQBiP35998.
PaxDbiP35998.
PeptideAtlasiP35998.
PRIDEiP35998.

2D gel databases

OGPiP35998.
REPRODUCTION-2DPAGEiIPI00021435.
P35998.

PTM databases

iPTMnetiP35998.
PhosphoSitePlusiP35998.
SwissPalmiP35998.

Expressioni

Gene expression databases

BgeeiENSG00000161057.
CleanExiHS_PSMC2.
ExpressionAtlasiP35998. baseline and differential.
GenevisibleiP35998. HS.

Organism-specific databases

HPAiHPA019238.
HPA049621.

Interactioni

Subunit structurei

Component of the 19S proteasome regulatory particle complex. The 26S proteasome consists of a 20S core particle (CP) and two 19S regulatory subunits (RP). The regulatory particle is made of a lid composed of 9 subunits, a base containing 6 ATPases including PSMC2 and few additional components (PubMed:27428775, PubMed:27342858). Interacts with NDC80 and SQSTM1 (PubMed:9295362, PubMed:10409732). Interacts with PAAF1 (PubMed:15831487). Interacts with HIV-1 Tat. Interacts with TRIM5 (PubMed:22078707).7 Publications

Binary interactionsi

Show more details

GO - Molecular functioni

Protein-protein interaction databases

BioGridi111674. 158 interactors.
DIPiDIP-27554N.
IntActiP35998. 56 interactors.
MINTiMINT-1163662.
STRINGi9606.ENSP00000292644.

Structurei

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
5GJQelectron microscopy4.50H1-433[»]
5GJRelectron microscopy3.50H/v1-433[»]
5L4Gelectron microscopy4.02H1-433[»]
5T0Celectron microscopy3.80AA/BA1-433[»]
5T0Gelectron microscopy4.40A1-433[»]
5T0Helectron microscopy6.80A1-433[»]
5T0Ielectron microscopy8.00A1-433[»]
5T0Jelectron microscopy8.00A1-433[»]
ProteinModelPortaliP35998.
SMRiP35998.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the AAA ATPase family.Curated

Phylogenomic databases

eggNOGiKOG0729. Eukaryota.
COG1222. LUCA.
GeneTreeiENSGT00550000074978.
HOGENOMiHOG000225143.
HOVERGENiHBG000109.
InParanoidiP35998.
KOiK03061.
OMAiFQVARCT.
OrthoDBiEOG091G096W.
PhylomeDBiP35998.
TreeFamiTF105661.

Family and domain databases

InterProiView protein in InterPro
IPR005937. 26S_Psome_P45.
IPR003593. AAA+_ATPase.
IPR003959. ATPase_AAA_core.
IPR003960. ATPase_AAA_CS.
IPR027417. P-loop_NTPase.
PfamiView protein in Pfam
PF00004. AAA. 1 hit.
SMARTiView protein in SMART
SM00382. AAA. 1 hit.
SUPFAMiSSF52540. SSF52540. 1 hit.
TIGRFAMsiTIGR01242. 26Sp45. 1 hit.
PROSITEiView protein in PROSITE
PS00674. AAA. 1 hit.

Sequences (2)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: P35998-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MPDYLGADQR KTKEDEKDDK PIRALDEGDI ALLKTYGQST YSRQIKQVED
60 70 80 90 100
DIQQLLKKIN ELTGIKESDT GLAPPALWDL AADKQTLQSE QPLQVARCTK
110 120 130 140 150
IINADSEDPK YIINVKQFAK FVVDLSDQVA PTDIEEGMRV GVDRNKYQIH
160 170 180 190 200
IPLPPKIDPT VTMMQVEEKP DVTYSDVGGC KEQIEKLREV VETPLLHPER
210 220 230 240 250
FVNLGIEPPK GVLLFGPPGT GKTLCARAVA NRTDACFIRV IGSELVQKYV
260 270 280 290 300
GEGARMVREL FEMARTKKAC LIFFDEIDAI GGARFDDGAG GDNEVQRTML
310 320 330 340 350
ELINQLDGFD PRGNIKVLMA TNRPDTLDPA LMRPGRLDRK IEFSLPDLEG
360 370 380 390 400
RTHIFKIHAR SMSVERDIRF ELLARLCPNS TGAEIRSVCT EAGMFAIRAR
410 420 430
RKIATEKDFL EAVNKVIKSY AKFSATPRYM TYN
Length:433
Mass (Da):48,634
Last modified:January 23, 2007 - v3
Checksum:i85FD95F6DF7A3E84
GO
Isoform 2 (identifier: P35998-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-137: Missing.

Note: No experimental confirmation available.
Show »
Length:296
Mass (Da):33,274
Checksum:iFB08EBF831391C10
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti15D → V in BAE45763 (PubMed:12880961).Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0561781 – 137Missing in isoform 2. 1 PublicationAdd BLAST137

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D11094 mRNA. Translation: BAA01868.1.
AB075520 mRNA. Translation: BAE45763.1.
AK298821 mRNA. Translation: BAH12878.1.
AC004668 Genomic DNA. No translation available.
AC093701 Genomic DNA. No translation available.
CH236947 Genomic DNA. Translation: EAL24412.1.
CH471070 Genomic DNA. Translation: EAW83332.1.
BC002589 mRNA. Translation: AAH02589.1.
CCDSiCCDS5731.1. [P35998-1]
PIRiS24353.
RefSeqiNP_001191382.1. NM_001204453.1.
NP_002794.1. NM_002803.3. [P35998-1]
UniGeneiHs.437366.

Genome annotation databases

EnsembliENST00000292644; ENSP00000292644; ENSG00000161057. [P35998-1]
ENST00000435765; ENSP00000391211; ENSG00000161057. [P35998-1]
GeneIDi5701.
KEGGihsa:5701.
UCSCiuc003vbs.4. human. [P35998-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D11094 mRNA. Translation: BAA01868.1.
AB075520 mRNA. Translation: BAE45763.1.
AK298821 mRNA. Translation: BAH12878.1.
AC004668 Genomic DNA. No translation available.
AC093701 Genomic DNA. No translation available.
CH236947 Genomic DNA. Translation: EAL24412.1.
CH471070 Genomic DNA. Translation: EAW83332.1.
BC002589 mRNA. Translation: AAH02589.1.
CCDSiCCDS5731.1. [P35998-1]
PIRiS24353.
RefSeqiNP_001191382.1. NM_001204453.1.
NP_002794.1. NM_002803.3. [P35998-1]
UniGeneiHs.437366.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
5GJQelectron microscopy4.50H1-433[»]
5GJRelectron microscopy3.50H/v1-433[»]
5L4Gelectron microscopy4.02H1-433[»]
5T0Celectron microscopy3.80AA/BA1-433[»]
5T0Gelectron microscopy4.40A1-433[»]
5T0Helectron microscopy6.80A1-433[»]
5T0Ielectron microscopy8.00A1-433[»]
5T0Jelectron microscopy8.00A1-433[»]
ProteinModelPortaliP35998.
SMRiP35998.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi111674. 158 interactors.
DIPiDIP-27554N.
IntActiP35998. 56 interactors.
MINTiMINT-1163662.
STRINGi9606.ENSP00000292644.

PTM databases

iPTMnetiP35998.
PhosphoSitePlusiP35998.
SwissPalmiP35998.

Polymorphism and mutation databases

BioMutaiPSMC2.
DMDMi547930.

2D gel databases

OGPiP35998.
REPRODUCTION-2DPAGEiIPI00021435.
P35998.

Proteomic databases

EPDiP35998.
MaxQBiP35998.
PaxDbiP35998.
PeptideAtlasiP35998.
PRIDEiP35998.

Protocols and materials databases

DNASUi5701.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000292644; ENSP00000292644; ENSG00000161057. [P35998-1]
ENST00000435765; ENSP00000391211; ENSG00000161057. [P35998-1]
GeneIDi5701.
KEGGihsa:5701.
UCSCiuc003vbs.4. human. [P35998-1]

Organism-specific databases

CTDi5701.
DisGeNETi5701.
GeneCardsiPSMC2.
HGNCiHGNC:9548. PSMC2.
HPAiHPA019238.
HPA049621.
MIMi154365. gene.
neXtProtiNX_P35998.
OpenTargetsiENSG00000161057.
PharmGKBiPA33893.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiKOG0729. Eukaryota.
COG1222. LUCA.
GeneTreeiENSGT00550000074978.
HOGENOMiHOG000225143.
HOVERGENiHBG000109.
InParanoidiP35998.
KOiK03061.
OMAiFQVARCT.
OrthoDBiEOG091G096W.
PhylomeDBiP35998.
TreeFamiTF105661.

Enzyme and pathway databases

ReactomeiR-HSA-1169091. Activation of NF-kappaB in B cells.
R-HSA-1234176. Oxygen-dependent proline hydroxylation of Hypoxia-inducible Factor Alpha.
R-HSA-1236974. ER-Phagosome pathway.
R-HSA-1236978. Cross-presentation of soluble exogenous antigens (endosomes).
R-HSA-174084. Autodegradation of Cdh1 by Cdh1:APC/C.
R-HSA-174113. SCF-beta-TrCP mediated degradation of Emi1.
R-HSA-174154. APC/C:Cdc20 mediated degradation of Securin.
R-HSA-174178. APC/C:Cdh1 mediated degradation of Cdc20 and other APC/C:Cdh1 targeted proteins in late mitosis/early G1.
R-HSA-174184. Cdc20:Phospho-APC/C mediated degradation of Cyclin A.
R-HSA-180534. Vpu mediated degradation of CD4.
R-HSA-180585. Vif-mediated degradation of APOBEC3G.
R-HSA-187577. SCF(Skp2)-mediated degradation of p27/p21.
R-HSA-195253. Degradation of beta-catenin by the destruction complex.
R-HSA-202424. Downstream TCR signaling.
R-HSA-211733. Regulation of activated PAK-2p34 by proteasome mediated degradation.
R-HSA-2467813. Separation of Sister Chromatids.
R-HSA-2871837. FCERI mediated NF-kB activation.
R-HSA-349425. Autodegradation of the E3 ubiquitin ligase COP1.
R-HSA-350562. Regulation of ornithine decarboxylase (ODC).
R-HSA-382556. ABC-family proteins mediated transport.
R-HSA-450408. AUF1 (hnRNP D0) binds and destabilizes mRNA.
R-HSA-4608870. Asymmetric localization of PCP proteins.
R-HSA-4641257. Degradation of AXIN.
R-HSA-4641258. Degradation of DVL.
R-HSA-5358346. Hedgehog ligand biogenesis.
R-HSA-5362768. Hh mutants that don't undergo autocatalytic processing are degraded by ERAD.
R-HSA-5607761. Dectin-1 mediated noncanonical NF-kB signaling.
R-HSA-5607764. CLEC7A (Dectin-1) signaling.
R-HSA-5610780. Degradation of GLI1 by the proteasome.
R-HSA-5610783. Degradation of GLI2 by the proteasome.
R-HSA-5610785. GLI3 is processed to GLI3R by the proteasome.
R-HSA-5632684. Hedgehog 'on' state.
R-HSA-5658442. Regulation of RAS by GAPs.
R-HSA-5668541. TNFR2 non-canonical NF-kB pathway.
R-HSA-5676590. NIK-->noncanonical NF-kB signaling.
R-HSA-5678895. Defective CFTR causes cystic fibrosis.
R-HSA-5687128. MAPK6/MAPK4 signaling.
R-HSA-5689603. UCH proteinases.
R-HSA-5689880. Ub-specific processing proteases.
R-HSA-6798695. Neutrophil degranulation.
R-HSA-68827. CDT1 association with the CDC6:ORC:origin complex.
R-HSA-68949. Orc1 removal from chromatin.
R-HSA-69017. CDK-mediated phosphorylation and removal of Cdc6.
R-HSA-69229. Ubiquitin-dependent degradation of Cyclin D1.
R-HSA-69481. G2/M Checkpoints.
R-HSA-69601. Ubiquitin Mediated Degradation of Phosphorylated Cdc25A.
R-HSA-8852276. The role of GTSE1 in G2/M progression after G2 checkpoint.
R-HSA-8854050. FBXL7 down-regulates AURKA during mitotic entry and in early mitosis.
R-HSA-983168. Antigen processing: Ubiquitination & Proteasome degradation.

Miscellaneous databases

ChiTaRSiPSMC2. human.
GeneWikiiPSMC2.
GenomeRNAii5701.
PROiPR:P35998.
SOURCEiSearch...

Gene expression databases

BgeeiENSG00000161057.
CleanExiHS_PSMC2.
ExpressionAtlasiP35998. baseline and differential.
GenevisibleiP35998. HS.

Family and domain databases

InterProiView protein in InterPro
IPR005937. 26S_Psome_P45.
IPR003593. AAA+_ATPase.
IPR003959. ATPase_AAA_core.
IPR003960. ATPase_AAA_CS.
IPR027417. P-loop_NTPase.
PfamiView protein in Pfam
PF00004. AAA. 1 hit.
SMARTiView protein in SMART
SM00382. AAA. 1 hit.
SUPFAMiSSF52540. SSF52540. 1 hit.
TIGRFAMsiTIGR01242. 26Sp45. 1 hit.
PROSITEiView protein in PROSITE
PS00674. AAA. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiPRS7_HUMAN
AccessioniPrimary (citable) accession number: P35998
Secondary accession number(s): A4D0Q1
, B7Z5E2, Q3LIA5, Q9UDI3
Entry historyiIntegrated into UniProtKB/Swiss-Prot: June 1, 1994
Last sequence update: January 23, 2007
Last modified: April 12, 2017
This is version 177 of the entry and version 3 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Human chromosome 7
    Human chromosome 7: entries, gene names and cross-references to MIM
  2. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  3. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  4. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.