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Protein

40S ribosomal protein S27-A

Gene

RPS27A

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Cofactori

Zn2+CuratedNote: Binds 1 zinc ion per subunit.Curated

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Zinc fingeri37 – 5923C4-typeSequence analysisAdd
BLAST

GO - Molecular functioni

  • metal ion binding Source: UniProtKB-KW
  • structural constituent of ribosome Source: SGD

GO - Biological processi

  • cytoplasmic translation Source: SGD
  • maturation of SSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) Source: SGD
  • ribosomal small subunit assembly Source: SGD
  • translation Source: GO_Central
Complete GO annotation...

Keywords - Molecular functioni

Ribonucleoprotein, Ribosomal protein

Keywords - Ligandi

Metal-binding, Zinc

Enzyme and pathway databases

BioCyciYEAST:G3O-31926-MONOMER.
ReactomeiR-SCE-156827. L13a-mediated translational silencing of Ceruloplasmin expression.
R-SCE-1799339. SRP-dependent cotranslational protein targeting to membrane.
R-SCE-72689. Formation of a pool of free 40S subunits.
R-SCE-72695. Formation of the ternary complex, and subsequently, the 43S complex.
R-SCE-72702. Ribosomal scanning and start codon recognition.
R-SCE-72706. GTP hydrolysis and joining of the 60S ribosomal subunit.
R-SCE-975956. Nonsense Mediated Decay (NMD) independent of the Exon Junction Complex (EJC).
R-SCE-975957. Nonsense Mediated Decay (NMD) enhanced by the Exon Junction Complex (EJC).

Names & Taxonomyi

Protein namesi
Recommended name:
40S ribosomal protein S27-A
Alternative name(s):
RP61
YS20
Gene namesi
Name:RPS27A
Ordered Locus Names:YKL156W
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome XI

Organism-specific databases

EuPathDBiFungiDB:YKL156W.
SGDiS000001639. RPS27A.

Subcellular locationi

GO - Cellular componenti

  • cytosolic small ribosomal subunit Source: SGD
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 828240S ribosomal protein S27-APRO_0000149066Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei40 – 401S-methylcysteine1 Publication

Post-translational modificationi

The N-terminus is not modified.

Keywords - PTMi

Methylation

Proteomic databases

TopDownProteomicsiP35997.

Interactioni

Subunit structurei

Component of the small ribosomal subunit. Mature ribosomes consist of a small (40S) and a large (60S) subunit. The 40S subunit contains 32 different proteins (encoded by 56 genes) and 1 molecule of RNA (18S). The 60S subunit contains 46 different proteins (encoded by 81 genes) and 3 molecules of RNA (25S, 5.8S and 5S).1 Publication

Protein-protein interaction databases

BioGridi33981. 35 interactions.
IntActiP35997. 6 interactions.
MINTiMINT-1325338.

Structurei

Secondary structure

1
82
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi7 – 93Combined sources
Helixi12 – 165Combined sources
Beta strandi22 – 254Combined sources
Beta strandi32 – 365Combined sources
Beta strandi40 – 423Combined sources
Beta strandi44 – 474Combined sources
Beta strandi57 – 593Combined sources
Beta strandi63 – 653Combined sources
Beta strandi68 – 703Combined sources
Beta strandi78 – 814Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
3J6Xelectron microscopy6.10271-82[»]
3J6Yelectron microscopy6.10271-82[»]
3J77electron microscopy6.20271-82[»]
3J78electron microscopy6.30271-82[»]
3V88X-ray3.00b1-82[»]
4U3MX-ray3.00D7/d72-82[»]
4U3NX-ray3.20D7/d72-82[»]
4U3UX-ray2.90D7/d72-82[»]
4U4NX-ray3.10D7/d72-82[»]
4U4OX-ray3.60D7/d72-82[»]
4U4QX-ray3.00D7/d72-82[»]
4U4RX-ray2.80D7/d72-82[»]
4U4UX-ray3.00D7/d72-82[»]
4U4YX-ray3.20D7/d72-82[»]
4U4ZX-ray3.10D7/d72-82[»]
4U50X-ray3.20D7/d72-82[»]
4U51X-ray3.20D7/d72-82[»]
4U52X-ray3.00D7/d72-82[»]
4U53X-ray3.30D7/d72-82[»]
4U55X-ray3.20D7/d72-82[»]
4U56X-ray3.45D7/d72-82[»]
4U6FX-ray3.10D7/d72-82[»]
4V6Ielectron microscopy8.80AX1-82[»]
4V88X-ray3.00Ab/Cb1-82[»]
4V8Yelectron microscopy4.30A11-82[»]
4V8Zelectron microscopy6.60A11-82[»]
4V92electron microscopy3.70b2-82[»]
5DC3X-ray3.25D7/d72-82[»]
5FCIX-ray3.40D7/d72-82[»]
5FCJX-ray3.10D7/d72-82[»]
5I4LX-ray3.10D7/d72-82[»]
ProteinModelPortaliP35997.
SMRiP35997. Positions 2-82.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the ribosomal protein S27e family.Curated

Zinc finger

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Zinc fingeri37 – 5923C4-typeSequence analysisAdd
BLAST

Keywords - Domaini

Zinc-finger

Phylogenomic databases

GeneTreeiENSGT00840000132030.
InParanoidiP35997.
KOiK02978.
OMAiFSHASMV.
OrthoDBiEOG092C5YES.

Family and domain databases

Gene3Di2.20.25.100. 1 hit.
HAMAPiMF_00371. Ribosomal_S27e. 1 hit.
InterProiIPR000592. Ribosomal_S27e.
IPR023407. Ribosomal_S27e_Zn-bd_dom.
IPR011332. Ribosomal_zn-bd.
[Graphical view]
PANTHERiPTHR11594. PTHR11594. 1 hit.
PfamiPF01667. Ribosomal_S27e. 1 hit.
[Graphical view]
ProDomiPD004466. Ribosomal_S27e. 1 hit.
[Graphical view] [Entries sharing at least one domain]
SUPFAMiSSF57829. SSF57829. 1 hit.
PROSITEiPS01168. RIBOSOMAL_S27E. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P35997-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MVLVQDLLHP TAASEARKHK LKTLVQGPRS YFLDVKCPGC LNITTVFSHA
60 70 80
QTAVTCESCS TILCTPTGGK AKLSEGTSFR RK
Length:82
Mass (Da):8,879
Last modified:June 1, 1994 - v1
Checksum:iFCA80130CFCEC91E
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z26877 Genomic DNA. No translation available.
Z28156 Genomic DNA. Translation: CAA81998.1.
Z28155 Genomic DNA. Translation: CAA81997.1.
BK006944 Genomic DNA. Translation: DAA09008.1.
PIRiS37986.
RefSeqiNP_012766.1. NM_001179722.1.

Genome annotation databases

EnsemblFungiiYKL156W; YKL156W; YKL156W.
GeneIDi853700.
KEGGisce:YKL156W.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z26877 Genomic DNA. No translation available.
Z28156 Genomic DNA. Translation: CAA81998.1.
Z28155 Genomic DNA. Translation: CAA81997.1.
BK006944 Genomic DNA. Translation: DAA09008.1.
PIRiS37986.
RefSeqiNP_012766.1. NM_001179722.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
3J6Xelectron microscopy6.10271-82[»]
3J6Yelectron microscopy6.10271-82[»]
3J77electron microscopy6.20271-82[»]
3J78electron microscopy6.30271-82[»]
3V88X-ray3.00b1-82[»]
4U3MX-ray3.00D7/d72-82[»]
4U3NX-ray3.20D7/d72-82[»]
4U3UX-ray2.90D7/d72-82[»]
4U4NX-ray3.10D7/d72-82[»]
4U4OX-ray3.60D7/d72-82[»]
4U4QX-ray3.00D7/d72-82[»]
4U4RX-ray2.80D7/d72-82[»]
4U4UX-ray3.00D7/d72-82[»]
4U4YX-ray3.20D7/d72-82[»]
4U4ZX-ray3.10D7/d72-82[»]
4U50X-ray3.20D7/d72-82[»]
4U51X-ray3.20D7/d72-82[»]
4U52X-ray3.00D7/d72-82[»]
4U53X-ray3.30D7/d72-82[»]
4U55X-ray3.20D7/d72-82[»]
4U56X-ray3.45D7/d72-82[»]
4U6FX-ray3.10D7/d72-82[»]
4V6Ielectron microscopy8.80AX1-82[»]
4V88X-ray3.00Ab/Cb1-82[»]
4V8Yelectron microscopy4.30A11-82[»]
4V8Zelectron microscopy6.60A11-82[»]
4V92electron microscopy3.70b2-82[»]
5DC3X-ray3.25D7/d72-82[»]
5FCIX-ray3.40D7/d72-82[»]
5FCJX-ray3.10D7/d72-82[»]
5I4LX-ray3.10D7/d72-82[»]
ProteinModelPortaliP35997.
SMRiP35997. Positions 2-82.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi33981. 35 interactions.
IntActiP35997. 6 interactions.
MINTiMINT-1325338.

Proteomic databases

TopDownProteomicsiP35997.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYKL156W; YKL156W; YKL156W.
GeneIDi853700.
KEGGisce:YKL156W.

Organism-specific databases

EuPathDBiFungiDB:YKL156W.
SGDiS000001639. RPS27A.

Phylogenomic databases

GeneTreeiENSGT00840000132030.
InParanoidiP35997.
KOiK02978.
OMAiFSHASMV.
OrthoDBiEOG092C5YES.

Enzyme and pathway databases

BioCyciYEAST:G3O-31926-MONOMER.
ReactomeiR-SCE-156827. L13a-mediated translational silencing of Ceruloplasmin expression.
R-SCE-1799339. SRP-dependent cotranslational protein targeting to membrane.
R-SCE-72689. Formation of a pool of free 40S subunits.
R-SCE-72695. Formation of the ternary complex, and subsequently, the 43S complex.
R-SCE-72702. Ribosomal scanning and start codon recognition.
R-SCE-72706. GTP hydrolysis and joining of the 60S ribosomal subunit.
R-SCE-975956. Nonsense Mediated Decay (NMD) independent of the Exon Junction Complex (EJC).
R-SCE-975957. Nonsense Mediated Decay (NMD) enhanced by the Exon Junction Complex (EJC).

Miscellaneous databases

PROiP35997.

Family and domain databases

Gene3Di2.20.25.100. 1 hit.
HAMAPiMF_00371. Ribosomal_S27e. 1 hit.
InterProiIPR000592. Ribosomal_S27e.
IPR023407. Ribosomal_S27e_Zn-bd_dom.
IPR011332. Ribosomal_zn-bd.
[Graphical view]
PANTHERiPTHR11594. PTHR11594. 1 hit.
PfamiPF01667. Ribosomal_S27e. 1 hit.
[Graphical view]
ProDomiPD004466. Ribosomal_S27e. 1 hit.
[Graphical view] [Entries sharing at least one domain]
SUPFAMiSSF57829. SSF57829. 1 hit.
PROSITEiPS01168. RIBOSOMAL_S27E. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiRS27_YEAST
AccessioniPrimary (citable) accession number: P35997
Secondary accession number(s): D6VX42
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 1, 1994
Last sequence update: June 1, 1994
Last modified: September 7, 2016
This is version 124 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 43300 molecules/cell in log phase SD medium.1 Publication
There are 2 genes for S27 in yeast.

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. Ribosomal proteins
    Ribosomal proteins families and list of entries
  3. SIMILARITY comments
    Index of protein domains and families
  4. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  5. Yeast chromosome XI
    Yeast (Saccharomyces cerevisiae) chromosome XI: entries and gene names

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.