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Protein

40S ribosomal protein S27-A

Gene

RPS27A

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Component of the ribosome, a large ribonucleoprotein complex responsible for the synthesis of proteins in the cell. The small ribosomal subunit (SSU) binds messenger RNAs (mRNAs) and translates the encoded message by selecting cognate aminoacyl-transfer RNA (tRNA) molecules. The large subunit (LSU) contains the ribosomal catalytic site termed the peptidyl transferase center (PTC), which catalyzes the formation of peptide bonds, thereby polymerizing the amino acids delivered by tRNAs into a polypeptide chain. The nascent polypeptides leave the ribosome through a tunnel in the LSU and interact with protein factors that function in enzymatic processing, targeting, and the membrane insertion of nascent chains at the exit of the ribosomal tunnel.1 Publication

Miscellaneous

Present with 43300 molecules/cell in log phase SD medium.1 Publication
There are 2 genes for eS27 in yeast.Curated

Cofactori

Zn2+CuratedNote: Binds 1 zinc ion per subunit.Curated

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri37 – 59C4-typeSequence analysisAdd BLAST23

GO - Molecular functioni

  • metal ion binding Source: UniProtKB-KW
  • structural constituent of ribosome Source: SGD

GO - Biological processi

  • cytoplasmic translation Source: SGD
  • maturation of SSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) Source: SGD
  • ribosomal small subunit assembly Source: SGD
  • translation Source: GO_Central

Keywordsi

Molecular functionRibonucleoprotein, Ribosomal protein
LigandMetal-binding, Zinc

Enzyme and pathway databases

BioCyciYEAST:G3O-31926-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
40S ribosomal protein S27-A1 Publication
Alternative name(s):
RP61
Small ribosomal subunit protein eS27-A1 Publication
YS20
Gene namesi
Name:RPS27A1 Publication
Ordered Locus Names:YKL156W
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome XI

Organism-specific databases

EuPathDBiFungiDB:YKL156W.
SGDiS000001639. RPS27A.

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001490661 – 8240S ribosomal protein S27-AAdd BLAST82

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei40S-methylcysteine1 Publication1

Post-translational modificationi

The N-terminus is not modified.1 Publication

Keywords - PTMi

Methylation

Proteomic databases

MaxQBiP35997.
PRIDEiP35997.
TopDownProteomicsiP35997.

Interactioni

Subunit structurei

Component of the small ribosomal subunit (SSU). Mature yeast ribosomes consist of a small (40S) and a large (60S) subunit. The 40S small subunit contains 1 molecule of ribosomal RNA (18S rRNA) and 33 different proteins (encoded by 57 genes). The large 60S subunit contains 3 rRNA molecules (25S, 5.8S and 5S rRNA) and 46 different proteins (encoded by 81 genes) (PubMed:9559554, PubMed:22096102).1 Publication1 Publication

Protein-protein interaction databases

BioGridi33981. 109 interactors.
IntActiP35997. 11 interactors.
MINTiMINT-1325338.
STRINGi4932.YKL156W.

Structurei

Secondary structure

182
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi7 – 9Combined sources3
Helixi12 – 16Combined sources5
Beta strandi22 – 25Combined sources4
Beta strandi32 – 36Combined sources5
Beta strandi40 – 42Combined sources3
Beta strandi44 – 47Combined sources4
Beta strandi57 – 59Combined sources3
Beta strandi63 – 65Combined sources3
Beta strandi68 – 70Combined sources3
Beta strandi78 – 81Combined sources4

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
3J6Xelectron microscopy6.10271-82[»]
3J6Yelectron microscopy6.10271-82[»]
3J77electron microscopy6.20271-82[»]
3J78electron microscopy6.30271-82[»]
3V88X-ray3.00b1-82[»]
4U3MX-ray3.00D7/d72-82[»]
4U3NX-ray3.20D7/d72-82[»]
4U3UX-ray2.90D7/d72-82[»]
4U4NX-ray3.10D7/d72-82[»]
4U4OX-ray3.60D7/d72-82[»]
4U4QX-ray3.00D7/d72-82[»]
4U4RX-ray2.80D7/d72-82[»]
4U4UX-ray3.00D7/d72-82[»]
4U4YX-ray3.20D7/d72-82[»]
4U4ZX-ray3.10D7/d72-82[»]
4U50X-ray3.20D7/d72-82[»]
4U51X-ray3.20D7/d72-82[»]
4U52X-ray3.00D7/d72-82[»]
4U53X-ray3.30D7/d72-82[»]
4U55X-ray3.20D7/d72-82[»]
4U56X-ray3.45D7/d72-82[»]
4U6FX-ray3.10D7/d72-82[»]
4V6Ielectron microscopy8.80AX1-82[»]
4V88X-ray3.00Ab/Cb1-82[»]
4V8Yelectron microscopy4.30A11-82[»]
4V8Zelectron microscopy6.60A11-82[»]
4V92electron microscopy3.70b2-82[»]
5DATX-ray3.15D7/d72-82[»]
5DC3X-ray3.25D7/d72-82[»]
5DGEX-ray3.45D7/d72-82[»]
5DGFX-ray3.30D7/d72-82[»]
5DGVX-ray3.10D7/d72-82[»]
5FCIX-ray3.40D7/d72-82[»]
5FCJX-ray3.10D7/d72-82[»]
5I4LX-ray3.10D7/d72-82[»]
5JUOelectron microscopy4.00YB1-82[»]
5JUPelectron microscopy3.50YB1-82[»]
5JUSelectron microscopy4.20YB1-82[»]
5JUTelectron microscopy4.00YB1-82[»]
5JUUelectron microscopy4.00YB1-82[»]
5LL6electron microscopy3.90f1-82[»]
5LYBX-ray3.25D7/d72-82[»]
5M1Jelectron microscopy3.30b22-82[»]
5MC6electron microscopy3.80f1-82[»]
5TGAX-ray3.30D7/d72-82[»]
5TGMX-ray3.50D7/d72-82[»]
5WYJelectron microscopy8.70Sc1-82[»]
5WYKelectron microscopy4.50Sc1-82[»]
ProteinModelPortaliP35997.
SMRiP35997.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Zinc finger

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri37 – 59C4-typeSequence analysisAdd BLAST23

Keywords - Domaini

Zinc-finger

Phylogenomic databases

InParanoidiP35997.
KOiK02978.
OMAiCDNCSTV.
OrthoDBiEOG092C5YES.

Family and domain databases

Gene3Di2.20.25.100. 1 hit.
HAMAPiMF_00371. Ribosomal_S27e. 1 hit.
InterProiView protein in InterPro
IPR000592. Ribosomal_S27e.
IPR023407. Ribosomal_S27e_Zn-bd_dom.
IPR011332. Ribosomal_zn-bd.
PANTHERiPTHR11594. PTHR11594. 1 hit.
PfamiView protein in Pfam
PF01667. Ribosomal_S27e. 1 hit.
ProDomiView protein in ProDom or Entries sharing at least one domain
PD004466. Ribosomal_S27e. 1 hit.
SUPFAMiSSF57829. SSF57829. 1 hit.
PROSITEiView protein in PROSITE
PS01168. RIBOSOMAL_S27E. 1 hit.

Sequencei

Sequence statusi: Complete.

P35997-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MVLVQDLLHP TAASEARKHK LKTLVQGPRS YFLDVKCPGC LNITTVFSHA
60 70 80
QTAVTCESCS TILCTPTGGK AKLSEGTSFR RK
Length:82
Mass (Da):8,879
Last modified:June 1, 1994 - v1
Checksum:iFCA80130CFCEC91E
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z26877 Genomic DNA. No translation available.
Z28156 Genomic DNA. Translation: CAA81998.1.
Z28155 Genomic DNA. Translation: CAA81997.1.
BK006944 Genomic DNA. Translation: DAA09008.1.
PIRiS37986.
RefSeqiNP_012766.1. NM_001179722.1.

Genome annotation databases

EnsemblFungiiYKL156W; YKL156W; YKL156W.
GeneIDi853700.
KEGGisce:YKL156W.

Similar proteinsi

Entry informationi

Entry nameiRS27A_YEAST
AccessioniPrimary (citable) accession number: P35997
Secondary accession number(s): D6VX42
Entry historyiIntegrated into UniProtKB/Swiss-Prot: June 1, 1994
Last sequence update: June 1, 1994
Last modified: September 27, 2017
This is version 134 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. Ribosomal proteins
    Ribosomal proteins families and list of entries
  3. SIMILARITY comments
    Index of protein domains and families
  4. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  5. Yeast chromosome XI
    Yeast (Saccharomyces cerevisiae) chromosome XI: entries and gene names