P35997 (RS27_YEAST) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
40S ribosomal protein S27-A

Alternative name(s):
RP61
YS20

Gene names

Name:	RPS27A
Ordered Locus Names:	YKL156W

Organism

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)

Taxonomic identifier

559292 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces

Protein attributes

Sequence length	82 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Cofactor	Binds 1 zinc ion per subunit Potential.
Subunit structure	Component of the small ribosomal subunit. Mature ribosomes consist of a small (40S) and a large (60S) subunit. The 40S subunit contains 32 different proteins (encoded by 56 genes) and 1 molecule of RNA (18S). The 60S subunit contains 46 different proteins (encoded by 81 genes) and 3 molecules of RNA (25S, 5.8S and 5S). Ref.4
Subcellular location	Cytoplasm Ref.6.
Post-translational modification	The N-terminus is not modified.
Miscellaneous	Present with 43300 molecules/cell in log phase SD medium. Ref.7 There are 2 genes for S27 in yeast.
Sequence similarities	Belongs to the ribosomal protein S27e family.

Ontologies

Keywords
Cellular component	Cytoplasm
Domain	Zinc-finger
Ligand	Metal-binding Zinc
Molecular function	Ribonucleoprotein Ribosomal protein
Technical term	3D-structure Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	cytoplasmic translation Inferred by curator. Source: SGD maturation of SSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) Inferred from genetic interaction. Source: SGD ribosomal small subunit assembly Inferred from mutant phenotype. Source: SGD
Cellular component	cytosolic small ribosomal subunit Inferred from direct assay. Source: SGD
Molecular function	metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW structural constituent of ribosome Inferred from direct assay. Source: SGD
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 82	82	40S ribosomal protein S27-A		PRO_0000149066
Regions
	Zinc finger	37 – 59	23	C4-type

Sequences

	Sequence		Length	Mass (Da)
	P35997 [UniParc]. Last modified June 1, 1994. Version 1. Checksum: FCA80130CFCEC91E	FASTA	82	8,879
	10 20 30 40 50 60 MVLVQDLLHP TAASEARKHK LKTLVQGPRS YFLDVKCPGC LNITTVFSHA QTAVTCESCS 70 80 TILCTPTGGK AKLSEGTSFR RK

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"DNA sequencing of a 36.2 kb fragment located between the FAS1 and LAP loci of chromosome XI of Saccharomyces cerevisiae." Vandenbol M., Bolle P.-A., Dion C., Portetelle D., Hilger F. Yeast 10:S35-S40(1994) [PubMed: 8091859] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 204508 / S288c.
[2]	"Complete DNA sequence of yeast chromosome XI." Dujon B., Alexandraki D., Andre B., Ansorge W., Baladron V., Ballesta J.P.G., Banrevi A., Bolle P.-A., Bolotin-Fukuhara M., Bossier P., Bou G., Boyer J., Buitrago M.J., Cheret G., Colleaux L., Daignan-Fornier B., del Rey F., Dion C. , Domdey H., Duesterhoeft A., Duesterhus S., Entian K.-D., Erfle H., Esteban P.F., Feldmann H., Fernandes L., Fobo G.M., Fritz C., Fukuhara H., Gabel C., Gaillon L., Garcia-Cantalejo J.M., Garcia-Ramirez J.J., Gent M.E., Ghazvini M., Goffeau A., Gonzalez A., Grothues D., Guerreiro P., Hegemann J.H., Hewitt N., Hilger F., Hollenberg C.P., Horaitis O., Indge K.J., Jacquier A., James C.M., Jauniaux J.-C., Jimenez A., Keuchel H., Kirchrath L., Kleine K., Koetter P., Legrain P., Liebl S., Louis E.J., Maia e Silva A., Marck C., Monnier A.-L., Moestl D., Mueller S., Obermaier B., Oliver S.G., Pallier C., Pascolo S., Pfeiffer F., Philippsen P., Planta R.J., Pohl F.M., Pohl T.M., Poehlmann R., Portetelle D., Purnelle B., Puzos V., Ramezani Rad M., Rasmussen S.W., Remacha M.A., Revuelta J.L., Richard G.-F., Rieger M., Rodrigues-Pousada C., Rose M., Rupp T., Santos M.A., Schwager C., Sensen C., Skala J., Soares H., Sor F., Stegemann J., Tettelin H., Thierry A., Tzermia M., Urrestarazu L.A., van Dyck L., van Vliet-Reedijk J.C., Valens M., Vandenbol M., Vilela C., Vissers S., von Wettstein D., Voss H., Wiemann S., Xu G., Zimmermann J., Haasemann M., Becker I., Mewes H.-W. Nature 369:371-378(1994) [PubMed: 8196765] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 204508 / S288c.
[3]	Saccharomyces Genome Database Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: ATCC 204508 / S288c.
[4]	"The list of cytoplasmic ribosomal proteins of Saccharomyces cerevisiae." Planta R.J., Mager W.H. Yeast 14:471-477(1998) [PubMed: 9559554] [Abstract] Cited for: NOMENCLATURE, SUBUNIT.
[5]	"The action of N-terminal acetyltransferases on yeast ribosomal proteins." Arnold R.J., Polevoda B., Reilly J.P., Sherman F. J. Biol. Chem. 274:37035-37040(1999) [PubMed: 10601260] [Abstract] Cited for: ANALYSIS OF N-TERMINUS.
[6]	"Global analysis of protein localization in budding yeast." Huh W.-K., Falvo J.V., Gerke L.C., Carroll A.S., Howson R.W., Weissman J.S., O'Shea E.K. Nature 425:686-691(2003) [PubMed: 14562095] [Abstract] Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
[7]	"Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed: 14562106] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

Z26877 Genomic DNA. No translation available.
Z28156 Genomic DNA. Translation: CAA81998.1.
Z28155 Genomic DNA. Translation: CAA81997.1.
BK006944 Genomic DNA. Translation: DAA09008.1.

PIR

S37986.

RefSeq

NP_012766.1. NM_001179722.1.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
3IZB	electron microscopy	-	X	1-82	[»]

ProteinModelPortal

P35997.

SMR

P35997. Positions 31-80.

ModBase

Search...

Protein-protein interaction databases

IntAct

P35997. 3 interactions.

MINT

MINT-1325338.

STRING

P35997.

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Genome annotation databases

EnsemblFungi

YKL156W; YKL156W; YKL156W.

GeneID

853700.

KEGG

sce:YKL156W.

Organism-specific databases

CYGD

YKL156w.

SGD

S000001639. RPS27A.

Phylogenomic databases

eggNOG

fuNOG11292.

GeneTree

EFGT00050000004951.

HOGENOM

HBG392194.

OMA

PSDEIYD.

OrthoDB

EOG4R274Q.

Gene expression databases

ArrayExpress

P35997.

Genevestigator

P35997.

GermOnline

YKL156W. Saccharomyces cerevisiae.

Family and domain databases

InterPro

IPR000592. Ribosomal_S27e.
IPR023407. Ribosomal_S27e_Zn-bd_dom.
IPR011332. Ribosomal_zn-bd_dom.
[Graphical view]

Gene3D

G3DSA:2.20.25.100. G3DSA:2.20.25.100. 1 hit.

KO

K02978.

PANTHER

PTHR11594. Ribosomal_S27E. 1 hit.

Pfam

PF01667. Ribosomal_S27e. 1 hit.
[Graphical view]

ProDom

PD004466. Ribosomal_S27e. 1 hit.
[Graphical view] [Entries sharing at least one domain]

SUPFAM

SSF57829. Ribosomal_zn-bd. 1 hit.

PROSITE

PS01168. RIBOSOMAL_S27E. 1 hit.
[Graphical view]

ProtoNet

Search...

Other

NextBio

974690.

Entry information

Entry name

RS27_YEAST

Accession

Primary (citable) accession number: P35997
Secondary accession number(s): D6VX42

Entry history

Integrated into UniProtKB/Swiss-Prot:	June 1, 1994
Last sequence update:	June 1, 1994
Last modified:	December 14, 2011
This is version 87 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program