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Protein

40S ribosomal protein S27-A

Gene

RPS27A

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Cofactori

Zn2+CuratedNote: Binds 1 zinc ion per subunit.Curated

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Zinc fingeri37 – 5923C4-typeSequence analysisAdd
BLAST

GO - Molecular functioni

  • metal ion binding Source: UniProtKB-KW
  • structural constituent of ribosome Source: SGD

GO - Biological processi

  • cytoplasmic translation Source: SGD
  • maturation of SSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) Source: SGD
  • ribosomal small subunit assembly Source: SGD
  • translation Source: GO_Central
Complete GO annotation...

Keywords - Molecular functioni

Ribonucleoprotein, Ribosomal protein

Keywords - Ligandi

Metal-binding, Zinc

Enzyme and pathway databases

BioCyciYEAST:G3O-31926-MONOMER.
ReactomeiR-SCE-156827. L13a-mediated translational silencing of Ceruloplasmin expression.
R-SCE-1799339. SRP-dependent cotranslational protein targeting to membrane.
R-SCE-72689. Formation of a pool of free 40S subunits.
R-SCE-72695. Formation of the ternary complex, and subsequently, the 43S complex.
R-SCE-72702. Ribosomal scanning and start codon recognition.
R-SCE-72706. GTP hydrolysis and joining of the 60S ribosomal subunit.
R-SCE-975956. Nonsense Mediated Decay (NMD) independent of the Exon Junction Complex (EJC).
R-SCE-975957. Nonsense Mediated Decay (NMD) enhanced by the Exon Junction Complex (EJC).

Names & Taxonomyi

Protein namesi
Recommended name:
40S ribosomal protein S27-A
Alternative name(s):
RP61
YS20
Gene namesi
Name:RPS27A
Ordered Locus Names:YKL156W
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome XI

Organism-specific databases

EuPathDBiFungiDB:YKL156W.
SGDiS000001639. RPS27A.

Subcellular locationi

GO - Cellular componenti

  • cytosolic small ribosomal subunit Source: SGD
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 828240S ribosomal protein S27-APRO_0000149066Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei40 – 401S-methylcysteine1 Publication

Post-translational modificationi

The N-terminus is not modified.

Keywords - PTMi

Methylation

Proteomic databases

TopDownProteomicsiP35997.

Interactioni

Subunit structurei

Component of the small ribosomal subunit. Mature ribosomes consist of a small (40S) and a large (60S) subunit. The 40S subunit contains 32 different proteins (encoded by 56 genes) and 1 molecule of RNA (18S). The 60S subunit contains 46 different proteins (encoded by 81 genes) and 3 molecules of RNA (25S, 5.8S and 5S).1 Publication

Protein-protein interaction databases

BioGridi33981. 35 interactions.
IntActiP35997. 6 interactions.
MINTiMINT-1325338.

Structurei

Secondary structure

1
82
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi7 – 93Combined sources
Helixi12 – 165Combined sources
Beta strandi22 – 254Combined sources
Beta strandi32 – 365Combined sources
Beta strandi40 – 423Combined sources
Beta strandi44 – 474Combined sources
Beta strandi57 – 593Combined sources
Beta strandi63 – 653Combined sources
Beta strandi68 – 703Combined sources
Beta strandi78 – 814Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
3J6Xelectron microscopy6.10271-82[»]
3J6Yelectron microscopy6.10271-82[»]
3J77electron microscopy6.20271-82[»]
3J78electron microscopy6.30271-82[»]
3V88X-ray3.00b1-82[»]
4U3MX-ray3.00D7/d72-82[»]
4U3NX-ray3.20D7/d72-82[»]
4U3UX-ray2.90D7/d72-82[»]
4U4NX-ray3.10D7/d72-82[»]
4U4OX-ray3.60D7/d72-82[»]
4U4QX-ray3.00D7/d72-82[»]
4U4RX-ray2.80D7/d72-82[»]
4U4UX-ray3.00D7/d72-82[»]
4U4YX-ray3.20D7/d72-82[»]
4U4ZX-ray3.10D7/d72-82[»]
4U50X-ray3.20D7/d72-82[»]
4U51X-ray3.20D7/d72-82[»]
4U52X-ray3.00D7/d72-82[»]
4U53X-ray3.30D7/d72-82[»]
4U55X-ray3.20D7/d72-82[»]
4U56X-ray3.45D7/d72-82[»]
4U6FX-ray3.10D7/d72-82[»]
4V6Ielectron microscopy8.80AX1-82[»]
4V88X-ray3.00Ab/Cb1-82[»]
4V8Yelectron microscopy4.30A11-82[»]
4V8Zelectron microscopy6.60A11-82[»]
4V92electron microscopy3.70b2-82[»]
5FCIX-ray3.40D7/d72-82[»]
5FCJX-ray3.10D7/d72-82[»]
ProteinModelPortaliP35997.
SMRiP35997. Positions 2-82.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the ribosomal protein S27e family.Curated

Zinc finger

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Zinc fingeri37 – 5923C4-typeSequence analysisAdd
BLAST

Keywords - Domaini

Zinc-finger

Phylogenomic databases

GeneTreeiENSGT00840000132030.
InParanoidiP35997.
KOiK02978.
OMAiMPLARDF.
OrthoDBiEOG7BS4PT.

Family and domain databases

Gene3Di2.20.25.100. 1 hit.
HAMAPiMF_00371. Ribosomal_S27e.
InterProiIPR000592. Ribosomal_S27e.
IPR023407. Ribosomal_S27e_Zn-bd_dom.
IPR011332. Ribosomal_zn-bd.
[Graphical view]
PANTHERiPTHR11594. PTHR11594. 1 hit.
PfamiPF01667. Ribosomal_S27e. 1 hit.
[Graphical view]
ProDomiPD004466. Ribosomal_S27e. 1 hit.
[Graphical view] [Entries sharing at least one domain]
SUPFAMiSSF57829. SSF57829. 1 hit.
PROSITEiPS01168. RIBOSOMAL_S27E. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P35997-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MVLVQDLLHP TAASEARKHK LKTLVQGPRS YFLDVKCPGC LNITTVFSHA
60 70 80
QTAVTCESCS TILCTPTGGK AKLSEGTSFR RK
Length:82
Mass (Da):8,879
Last modified:June 1, 1994 - v1
Checksum:iFCA80130CFCEC91E
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z26877 Genomic DNA. No translation available.
Z28156 Genomic DNA. Translation: CAA81998.1.
Z28155 Genomic DNA. Translation: CAA81997.1.
BK006944 Genomic DNA. Translation: DAA09008.1.
PIRiS37986.
RefSeqiNP_012766.1. NM_001179722.1.

Genome annotation databases

EnsemblFungiiYKL156W; YKL156W; YKL156W.
GeneIDi853700.
KEGGisce:YKL156W.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z26877 Genomic DNA. No translation available.
Z28156 Genomic DNA. Translation: CAA81998.1.
Z28155 Genomic DNA. Translation: CAA81997.1.
BK006944 Genomic DNA. Translation: DAA09008.1.
PIRiS37986.
RefSeqiNP_012766.1. NM_001179722.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
3J6Xelectron microscopy6.10271-82[»]
3J6Yelectron microscopy6.10271-82[»]
3J77electron microscopy6.20271-82[»]
3J78electron microscopy6.30271-82[»]
3V88X-ray3.00b1-82[»]
4U3MX-ray3.00D7/d72-82[»]
4U3NX-ray3.20D7/d72-82[»]
4U3UX-ray2.90D7/d72-82[»]
4U4NX-ray3.10D7/d72-82[»]
4U4OX-ray3.60D7/d72-82[»]
4U4QX-ray3.00D7/d72-82[»]
4U4RX-ray2.80D7/d72-82[»]
4U4UX-ray3.00D7/d72-82[»]
4U4YX-ray3.20D7/d72-82[»]
4U4ZX-ray3.10D7/d72-82[»]
4U50X-ray3.20D7/d72-82[»]
4U51X-ray3.20D7/d72-82[»]
4U52X-ray3.00D7/d72-82[»]
4U53X-ray3.30D7/d72-82[»]
4U55X-ray3.20D7/d72-82[»]
4U56X-ray3.45D7/d72-82[»]
4U6FX-ray3.10D7/d72-82[»]
4V6Ielectron microscopy8.80AX1-82[»]
4V88X-ray3.00Ab/Cb1-82[»]
4V8Yelectron microscopy4.30A11-82[»]
4V8Zelectron microscopy6.60A11-82[»]
4V92electron microscopy3.70b2-82[»]
5FCIX-ray3.40D7/d72-82[»]
5FCJX-ray3.10D7/d72-82[»]
ProteinModelPortaliP35997.
SMRiP35997. Positions 2-82.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi33981. 35 interactions.
IntActiP35997. 6 interactions.
MINTiMINT-1325338.

Proteomic databases

TopDownProteomicsiP35997.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYKL156W; YKL156W; YKL156W.
GeneIDi853700.
KEGGisce:YKL156W.

Organism-specific databases

EuPathDBiFungiDB:YKL156W.
SGDiS000001639. RPS27A.

Phylogenomic databases

GeneTreeiENSGT00840000132030.
InParanoidiP35997.
KOiK02978.
OMAiMPLARDF.
OrthoDBiEOG7BS4PT.

Enzyme and pathway databases

BioCyciYEAST:G3O-31926-MONOMER.
ReactomeiR-SCE-156827. L13a-mediated translational silencing of Ceruloplasmin expression.
R-SCE-1799339. SRP-dependent cotranslational protein targeting to membrane.
R-SCE-72689. Formation of a pool of free 40S subunits.
R-SCE-72695. Formation of the ternary complex, and subsequently, the 43S complex.
R-SCE-72702. Ribosomal scanning and start codon recognition.
R-SCE-72706. GTP hydrolysis and joining of the 60S ribosomal subunit.
R-SCE-975956. Nonsense Mediated Decay (NMD) independent of the Exon Junction Complex (EJC).
R-SCE-975957. Nonsense Mediated Decay (NMD) enhanced by the Exon Junction Complex (EJC).

Miscellaneous databases

PROiP35997.

Family and domain databases

Gene3Di2.20.25.100. 1 hit.
HAMAPiMF_00371. Ribosomal_S27e.
InterProiIPR000592. Ribosomal_S27e.
IPR023407. Ribosomal_S27e_Zn-bd_dom.
IPR011332. Ribosomal_zn-bd.
[Graphical view]
PANTHERiPTHR11594. PTHR11594. 1 hit.
PfamiPF01667. Ribosomal_S27e. 1 hit.
[Graphical view]
ProDomiPD004466. Ribosomal_S27e. 1 hit.
[Graphical view] [Entries sharing at least one domain]
SUPFAMiSSF57829. SSF57829. 1 hit.
PROSITEiPS01168. RIBOSOMAL_S27E. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "DNA sequencing of a 36.2 kb fragment located between the FAS1 and LAP loci of chromosome XI of Saccharomyces cerevisiae."
    Vandenbol M., Bolle P.-A., Dion C., Portetelle D., Hilger F.
    Yeast 10:S35-S40(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  2. "Complete DNA sequence of yeast chromosome XI."
    Dujon B., Alexandraki D., Andre B., Ansorge W., Baladron V., Ballesta J.P.G., Banrevi A., Bolle P.-A., Bolotin-Fukuhara M., Bossier P., Bou G., Boyer J., Buitrago M.J., Cheret G., Colleaux L., Daignan-Fornier B., del Rey F., Dion C.
    , Domdey H., Duesterhoeft A., Duesterhus S., Entian K.-D., Erfle H., Esteban P.F., Feldmann H., Fernandes L., Fobo G.M., Fritz C., Fukuhara H., Gabel C., Gaillon L., Garcia-Cantalejo J.M., Garcia-Ramirez J.J., Gent M.E., Ghazvini M., Goffeau A., Gonzalez A., Grothues D., Guerreiro P., Hegemann J.H., Hewitt N., Hilger F., Hollenberg C.P., Horaitis O., Indge K.J., Jacquier A., James C.M., Jauniaux J.-C., Jimenez A., Keuchel H., Kirchrath L., Kleine K., Koetter P., Legrain P., Liebl S., Louis E.J., Maia e Silva A., Marck C., Monnier A.-L., Moestl D., Mueller S., Obermaier B., Oliver S.G., Pallier C., Pascolo S., Pfeiffer F., Philippsen P., Planta R.J., Pohl F.M., Pohl T.M., Poehlmann R., Portetelle D., Purnelle B., Puzos V., Ramezani Rad M., Rasmussen S.W., Remacha M.A., Revuelta J.L., Richard G.-F., Rieger M., Rodrigues-Pousada C., Rose M., Rupp T., Santos M.A., Schwager C., Sensen C., Skala J., Soares H., Sor F., Stegemann J., Tettelin H., Thierry A., Tzermia M., Urrestarazu L.A., van Dyck L., van Vliet-Reedijk J.C., Valens M., Vandenbol M., Vilela C., Vissers S., von Wettstein D., Voss H., Wiemann S., Xu G., Zimmermann J., Haasemann M., Becker I., Mewes H.-W.
    Nature 369:371-378(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  3. Cited for: GENOME REANNOTATION.
    Strain: ATCC 204508 / S288c.
  4. "The list of cytoplasmic ribosomal proteins of Saccharomyces cerevisiae."
    Planta R.J., Mager W.H.
    Yeast 14:471-477(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: NOMENCLATURE, SUBUNIT.
  5. "The action of N-terminal acetyltransferases on yeast ribosomal proteins."
    Arnold R.J., Polevoda B., Reilly J.P., Sherman F.
    J. Biol. Chem. 274:37035-37040(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: ANALYSIS OF N-TERMINUS.
  6. Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
  7. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
  8. "Identification of methylated proteins in the yeast small ribosomal subunit: a role for SPOUT methyltransferases in protein arginine methylation."
    Young B.D., Weiss D.I., Zurita-Lopez C.I., Webb K.J., Clarke S.G., McBride A.E.
    Biochemistry 51:5091-5104(2012) [PubMed] [Europe PMC] [Abstract]
    Cited for: METHYLATION AT CYS-40.
  9. Cited for: STRUCTURE BY ELECTRON MICROSCOPY.
  10. Cited for: X-RAY CRYSTALLOGRAPHY (3.00 ANGSTROMS).
  11. "Molecular architecture of a eukaryotic translational initiation complex."
    Fernandez I.S., Bai X.C., Hussain T., Kelley A.C., Lorsch J.R., Ramakrishnan V., Scheres S.H.
    Science 342:1240585-1240585(2013) [PubMed] [Europe PMC] [Abstract]
    Cited for: STRUCTURE BY ELECTRON MICROSCOPY (4.30 ANGSTROMS).

Entry informationi

Entry nameiRS27_YEAST
AccessioniPrimary (citable) accession number: P35997
Secondary accession number(s): D6VX42
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 1, 1994
Last sequence update: June 1, 1994
Last modified: July 6, 2016
This is version 123 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 43300 molecules/cell in log phase SD medium.1 Publication
There are 2 genes for S27 in yeast.

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. Ribosomal proteins
    Ribosomal proteins families and list of entries
  3. SIMILARITY comments
    Index of protein domains and families
  4. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  5. Yeast chromosome XI
    Yeast (Saccharomyces cerevisiae) chromosome XI: entries and gene names

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.