Reviewed, UniProtKB/Swiss-Prot P35997 (RS27A_YEAST)

Names and origin

Protein names

Recommended name:
    40S ribosomal protein S27-A
Alternative name(s):
    YS20
    RP61

Gene names

Name:	RPS27A
Ordered Locus Names:	YKL156W

Organism

Saccharomyces cerevisiae (Baker's yeast) [Complete proteome]

Taxonomic identifier

4932 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces

Protein attributes

Sequence length	82 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Cofactor	Binds 1 zinc ion per subunit Potential.
Subunit structure	Component of the small ribosomal subunit. Mature ribosomes consist of a small (40S) and a large (60S) subunit. The 40S subunit contains 32 different proteins (encoded by 56 genes) and 1 molecule of RNA (18S). The 60S subunit contains 46 different proteins (encoded by 81 genes) and 3 molecules of RNA (25S, 5.8S and 5S). Ref.3
Subcellular location	Cytoplasm.
Post-translational modification	The N-terminus is not modified.
Miscellaneous	Present with 43300 molecules/cell in log phase SD medium. Ref.6 There are 2 genes for S27 in yeast.
Sequence similarities	Belongs to the ribosomal protein S27e family.

Ontologies

Keywords
Cellular component	Cytoplasm
Domain	Zinc-finger
Ligand	Metal-binding Zinc
Molecular function	Ribonucleoprotein Ribosomal protein
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	maturation of SSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) Inferred from genetic interaction. Source: SGD ribosomal small subunit assembly Inferred from mutant phenotype. Source: SGD translation Inferred by curator. Source: SGD
Cellular component	cytosolic small ribosomal subunit Inferred from direct assay. Source: SGD
Molecular function	structural constituent of ribosome Inferred from direct assay. Source: SGD zinc ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 82	82	40S ribosomal protein S27-A		PRO_0000149066
Regions
	Zinc finger	37 – 59	23	C4-type

Sequences

	Sequence		Length	Mass (Da)
	P35997-1 [UniParc]. Last modified June 1, 1994. Version 1. Checksum: FCA80130CFCEC91E	FASTA	82	8,879
	10 20 30 40 50 60 MVLVQDLLHP TAASEARKHK LKTLVQGPRS YFLDVKCPGC LNITTVFSHA QTAVTCESCS 70 80 TILCTPTGGK AKLSEGTSFR RK

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"DNA sequencing of a 36.2 kb fragment located between the FAS1 and LAP loci of chromosome XI of Saccharomyces cerevisiae." Vandenbol M., Bolle P.-A., Dion C., Portetelle D., Hilger F. Yeast 10:S35-S40(1994) [PubMed: 8091859] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 204508 / S288c.
[2]	"Complete DNA sequence of yeast chromosome XI." Dujon B., Alexandraki D., Andre B., Ansorge W., Baladron V., Ballesta J.P.G., Banrevi A., Bolle P.-A., Bolotin-Fukuhara M., Bossier P., Bou G., Boyer J., Buitrago M.J., Cheret G., Colleaux L., Daignan-Fornier B., del Rey F., Dion C. , Domdey H., Duesterhoeft A., Duesterhus S., Entian K.-D., Erfle H., Esteban P.F., Feldmann H., Fernandes L., Fobo G.M., Fritz C., Fukuhara H., Gabel C., Gaillon L., Garcia-Cantalejo J.M., Garcia-Ramirez J.J., Gent M.E., Ghazvini M., Goffeau A., Gonzalez A., Grothues D., Guerreiro P., Hegemann J.H., Hewitt N., Hilger F., Hollenberg C.P., Horaitis O., Indge K.J., Jacquier A., James C.M., Jauniaux J.-C., Jimenez A., Keuchel H., Kirchrath L., Kleine K., Koetter P., Legrain P., Liebl S., Louis E.J., Maia e Silva A., Marck C., Monnier A.-L., Moestl D., Mueller S., Obermaier B., Oliver S.G., Pallier C., Pascolo S., Pfeiffer F., Philippsen P., Planta R.J., Pohl F.M., Pohl T.M., Poehlmann R., Portetelle D., Purnelle B., Puzos V., Ramezani Rad M., Rasmussen S.W., Remacha M.A., Revuelta J.L., Richard G.-F., Rieger M., Rodrigues-Pousada C., Rose M., Rupp T., Santos M.A., Schwager C., Sensen C., Skala J., Soares H., Sor F., Stegemann J., Tettelin H., Thierry A., Tzermia M., Urrestarazu L.A., van Dyck L., van Vliet-Reedijk J.C., Valens M., Vandenbol M., Vilela C., Vissers S., von Wettstein D., Voss H., Wiemann S., Xu G., Zimmermann J., Haasemann M., Becker I., Mewes H.-W. Nature 369:371-378(1994) [PubMed: 8196765] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 204508 / S288c.
[3]	"The list of cytoplasmic ribosomal proteins of Saccharomyces cerevisiae." Planta R.J., Mager W.H. Yeast 14:471-477(1998) [PubMed: 9559554] [Abstract] Cited for: NOMENCLATURE, SUBUNIT.
[4]	"The action of N-terminal acetyltransferases on yeast ribosomal proteins." Arnold R.J., Polevoda B., Reilly J.P., Sherman F. J. Biol. Chem. 274:37035-37040(1999) [PubMed: 10601260] [Abstract] Cited for: ANALYSIS OF N-TERMINUS.
[5]	"Global analysis of protein localization in budding yeast." Huh W.-K., Falvo J.V., Gerke L.C., Carroll A.S., Howson R.W., Weissman J.S., O'Shea E.K. Nature 425:686-691(2003) [PubMed: 14562095] [Abstract] Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
[6]	"Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed: 14562106] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBLGenBankDDBJ	Z26877 Genomic DNA. No translation available. Z28156 Genomic DNA. Translation: CAA81998.1. Z28155 Genomic DNA. Translation: CAA81997.1.
PIR	S37986.
RefSeq	NP_012766.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
IntAct	P35997. 3 interactions.
STRING	P35997.
Genome annotation databases
Ensembl	YKL156W; YKL156W; YKL156W; Saccharomyces cerevisiae. [Genome view]
GeneID	853700.
KEGG	sce:YKL156W.
Organism-specific databases
CYGD	YKL156w.
SGD	S000001639. RPS27A.
Phylogenomic databases
HOGENOM	P35997.
OMA	PSDEIYD
OrthoDB	EOG9PP00P
Gene expression databases
ArrayExpress	P35997.
Genevestigator	P35997.
GermOnline	YKL156W. Saccharomyces cerevisiae.
Family and domain databases
InterPro	IPR000592. Ribosomal_S27e. IPR011332. Ribosomal_zn-bd. [Graphical view]
Gene3D	G3DSA:3.10.330.30. Ribosomal_S27E. 1 hit.
PANTHER	PTHR11594. Ribosomal_S27E. 1 hit.
Pfam	PF01667. Ribosomal_S27e. 1 hit. [Graphical view]
ProDom	PD004466. Ribosomal_S27e. 1 hit. [Graphical view] [Entries sharing at least one domain]
PROSITE	PS01168. RIBOSOMAL_S27E. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	974690.

Entry information

Entry name

RS27A_YEAST

Accession

Primary (citable) accession number: P35997

Entry history

Integrated into UniProtKB/Swiss-Prot:	June 1, 1994
Last sequence update:	June 1, 1994
Last modified:	November 24, 2009
This is version 67 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

FPAP (Fungal Proteome Annotation Project)