Reviewed, UniProtKB/Swiss-Prot P35970 (DNLI_ASFB7)

Names and origin

Protein names

Recommended name:
    DNA ligase
    EC=6.5.1.1
Alternative name(s):
    Polydeoxyribonucleotide synthase [ATP]

Gene names

Name:	LIG
Ordered Locus Names:	Ba71V-100
ORF Names:	NP419L

Organism

African swine fever virus (strain Badajoz 1971 Vero-adapted) (Ba71V) (ASFV) [Complete proteome]

Taxonomic identifier

10498 [NCBI]

Taxonomic lineage

Viruses › dsDNA viruses, no RNA stage › Asfarviridae › Asfivirus

Virus host

Ornithodoros (relapsing fever ticks) [TaxID: 6937]
Sus scrofa (Pig) [TaxID: 9823]

Protein attributes

Sequence length	419 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Very low-fidelity DNA ligase that seals, during DNA repair, nicks in double-stranded DNA. Together with the viral repair DNA polymerase X, fills the single nucleotide gaps generated by the AP endonuclease. It is not essential for viral replication and recombination.
Catalytic activity	ATP + (deoxyribonucleotide)(n) + (deoxyribonucleotide)(m) = AMP + diphosphate + (deoxyribonucleotide)(n+m).
Cofactor	Divalent metal cations By similarity.
Miscellaneous	Consistent with its intracellular location, ASFV encodes its own replicative DNA polymerase and three base excision repair enzymes: a class II AP endonuclease, the repair polymerase Pol X, and an ATP-dependent DNA ligase.
Sequence similarities	Belongs to the ATP-dependent DNA ligase family.

Ontologies

Keywords
Biological process	Cell cycle Cell division DNA damage DNA recombination DNA repair DNA replication
Ligand	ATP-binding Metal-binding Nucleotide-binding
Molecular function	Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	DNA recombination Inferred from electronic annotation. Source: UniProtKB-KW DNA repair Inferred from electronic annotation. Source: UniProtKB-KW DNA replication Inferred from electronic annotation. Source: UniProtKB-KW cell cycle Inferred from electronic annotation. Source: UniProtKB-KW cell division Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW DNA ligase (ATP) activity Inferred from electronic annotation. Source: EC metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 419	419	DNA ligase		PRO_0000059592
Sites
	Active site	151	1	N6-AMP-lysine intermediate By similarity
	Metal binding	203	1	Divalent metal cation 1 By similarity
	Metal binding	291	1	Divalent metal cation 2 By similarity
	Binding site	156	1	ATP By similarity
	Binding site	172	1	ATP By similarity
	Binding site	203	1	ATP By similarity
	Binding site	232	1	ATP By similarity
	Binding site	316	1	ATP By similarity

Sequences

	Sequence		Length	Mass (Da)
	P35970-1 [UniParc]. Last modified June 1, 1994. Version 1. Checksum: 491A25A4B9CFD807	FASTA	419	48,164
	10 20 30 40 50 60 MLNQFPGQYS NNIFCFPPIE SETKSGKKAS WIICVQVVQH NTIIPITDEM FSTDVKDAVA 70 80 90 100 110 120 EIFTKFFVEE GAVRISKMTR VTEGKNLGKK NATTVVHQAF KDALSKYNRH ARQKRGAHTN 130 140 150 160 170 180 RGMIPPMLVK YFNIIPKTFF EEETDPIVQR KRNGVRAVAC QQGDGCILLY SRTEKEFLGL 190 200 210 220 230 240 DNIKKELKQL YLFIDVRVYL DGELYLHRKP LQWIAGQANA KTDSSELHFY VFDCFWSDQL 250 260 270 280 290 300 QMPSNKRQQL LTNIFKQKED LTFIHQVENF SVKNVDEALR LKAQFIKEGY EGAIVRNANG 310 320 330 340 350 360 PYEPGYNNYH SAHLAKLKPL LDAEFILVDY TQGKKGKDLG AILWVCELPN KKRFVVTPKH 370 380 390 400 410 LTYADRYALF QKLTPALFKK HLYGKELTVE YAELSPKTGI PLQARAVGFR EPINVLEII

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"African swine fever virus encodes a DNA ligase." Yanez R.J., Vinuela E. Virology 193:531-536(1993) [PubMed: 8438592] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"Analysis of the complete nucleotide sequence of African swine fever virus." Yanez R.J., Rodriguez J.M., Nogal M.L., Yuste L., Enriquez C., Rodriguez J.F., Vinuela E. Virology 208:249-278(1995) [PubMed: 11831707] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[3]	"An error-prone viral DNA ligase." Lamarche B.J., Showalter A.K., Tsai M.-D. Biochemistry 44:8408-8417(2005) [PubMed: 15938630] [Abstract] Cited for: CHARACTERIZATION.

Cross-references

Sequence databases
EMBLGenBankDDBJ	X65373 Genomic DNA. Translation: CAA46445.1. U18466 Genomic DNA. Translation: AAA65329.1.
PIR	A45397.
RefSeq	NP_042793.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	1488864.
Family and domain databases
InterPro	IPR012310. DNA_ligase_A_M. IPR016059. DNA_ligase_CS. [Graphical view]
Pfam	PF01068. DNA_ligase_A_M. 1 hit. [Graphical view]
PROSITE	PS00697. DNA_LIGASE_A1. 1 hit. PS00333. DNA_LIGASE_A2. 1 hit. PS50160. DNA_LIGASE_A3. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

DNLI_ASFB7

Accession

Primary (citable) accession number: P35970

Entry history

Integrated into UniProtKB/Swiss-Prot:	June 1, 1994
Last sequence update:	June 1, 1994
Last modified:	June 16, 2009
This is version 50 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

Virus (Virus annotation project)