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P35952 (LDLR_RAT) Reviewed, UniProtKB/Swiss-Prot

Last modified April 3, 2013. Version 121. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
Low-density lipoprotein receptor
Short name=LDL receptor
Gene names
Name:Ldlr
OrganismRattus norvegicus (Rat) [Reference proteome]
Taxonomic identifier10116 [NCBI]
Taxonomic lineageEukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Rattus

Protein attributes

Sequence length879 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at transcript level

General annotation (Comments)

Function

Binds LDL, the major cholesterol-carrying lipoprotein of plasma, and transports it into cells by endocytosis. In order to be internalized, the receptor-ligand complexes must first cluster into clathrin-coated pits.

Subunit structure

Interacts with LDLRAP1, ARRB1, SNX17. Interacts (via NPXY motif) with DAB2 (via PID domain); the interaction is impaired by tyrosine phosphorylation of the NPXY motif. Interacts with the full length immature form of PCSK9 (via C-terminus) By similarity.

Subcellular location

Cell membrane; Single-pass type I membrane protein. Endomembrane system; Single-pass type I membrane protein. Membrane › clathrin-coated pit; Single-pass type I membrane protein By similarity. Golgi apparatus By similarity. Early endosome By similarity. Late endosome By similarity. Cell surface By similarity. Lysosome By similarity. Note: Found distributed from the plasma membrane to intracellular compartments. Localizes to the Golgi apparatus, early and late endosomes/lysosomes and cell surface in the presence of PCSK9 By similarity.

Domain

The NPXY motif mediates the interaction with the clathrin adaptor DAB2 involved in receptor internalization By similarity.

Post-translational modification

Ubiquitinated by MYLIP leading to degradation By similarity.

Sequence similarities

Belongs to the LDLR family.

Contains 3 EGF-like domains.

Contains 7 LDL-receptor class A domains.

Contains 6 LDL-receptor class B repeats.

Ontologies

Keywords
   Biological processCholesterol metabolism
Endocytosis
Lipid metabolism
Lipid transport
Steroid metabolism
Sterol metabolism
Transport
   Cellular componentCell membrane
Coated pit
Endosome
Golgi apparatus
LDL
Lysosome
Membrane
   DomainEGF-like domain
Repeat
Signal
Transmembrane
Transmembrane helix
   Molecular functionReceptor
   PTMDisulfide bond
Glycoprotein
Ubl conjugation
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processaging

Inferred from expression pattern PubMed 16741953. Source: RGD

cholesterol metabolic process

Inferred from electronic annotation. Source: UniProtKB-KW

lipid transport

Inferred from electronic annotation. Source: UniProtKB-KW

response to estradiol stimulus

Inferred from expression pattern PubMed 19008317PubMed 3559401. Source: RGD

response to glucagon stimulus

Inferred from expression pattern PubMed 19008317. Source: RGD

response to hypoxia

Inferred from expression pattern PubMed 18703410. Source: RGD

   Cellular_componentGolgi apparatus

Inferred from sequence or structural similarity. Source: UniProtKB

caveola

Inferred from direct assay PubMed 12646183. Source: RGD

cell surface

Inferred from sequence or structural similarity. Source: UniProtKB

coated pit

Inferred from electronic annotation. Source: UniProtKB-SubCell

early endosome

Inferred from sequence or structural similarity. Source: UniProtKB

integral to membrane

Inferred from electronic annotation. Source: UniProtKB-KW

late endosome

Inferred from sequence or structural similarity. Source: UniProtKB

low-density lipoprotein particle

Inferred from electronic annotation. Source: UniProtKB-KW

lysosome

Inferred from sequence or structural similarity. Source: UniProtKB

recycling endosome membrane

Inferred from direct assay PubMed 10468577. Source: RGD

   Molecular_functioncalcium ion binding

Inferred from electronic annotation. Source: InterPro

low-density lipoprotein receptor activity

Inferred from mutant phenotype PubMed 3559401. Source: RGD

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 2121 By similarity
Chain22 – 879858Low-density lipoprotein receptor
PRO_0000017314

Regions

Topological domain22 – 807786Extracellular Potential
Transmembrane808 – 82922Helical; Potential
Topological domain830 – 87950Cytoplasmic Potential
Domain25 – 6541LDL-receptor class A 1
Domain66 – 10641LDL-receptor class A 2
Domain107 – 14539LDL-receptor class A 3
Domain146 – 18641LDL-receptor class A 4
Domain196 – 23439LDL-receptor class A 5
Domain235 – 27339LDL-receptor class A 6
Domain275 – 31440LDL-receptor class A 7
Domain315 – 35440EGF-like 1
Domain355 – 39440EGF-like 2; calcium-binding Potential
Repeat398 – 43942LDL-receptor class B 1
Repeat440 – 48546LDL-receptor class B 2
Repeat486 – 52843LDL-receptor class B 3
Repeat529 – 57244LDL-receptor class B 4
Repeat573 – 61543LDL-receptor class B 5
Repeat616 – 65843LDL-receptor class B 6
Domain663 – 71250EGF-like 3
Region717 – 78872Clustered O-linked oligosaccharides
Region830 – 87950Required for MYLIP-triggered down-regulation of LDLR By similarity
Motif842 – 8476NPXY motif

Amino acid modifications

Glycosylation971N-linked (GlcNAc...) Potential
Glycosylation1561N-linked (GlcNAc...) Potential
Glycosylation2731N-linked (GlcNAc...) Potential
Glycosylation6571N-linked (GlcNAc...) Potential
Disulfide bond27 ↔ 39 By similarity
Disulfide bond34 ↔ 52 By similarity
Disulfide bond46 ↔ 63 By similarity
Disulfide bond68 ↔ 82 By similarity
Disulfide bond75 ↔ 95 By similarity
Disulfide bond89 ↔ 104 By similarity
Disulfide bond109 ↔ 121 By similarity
Disulfide bond116 ↔ 134 By similarity
Disulfide bond128 ↔ 143 By similarity
Disulfide bond148 ↔ 160 By similarity
Disulfide bond155 ↔ 173 By similarity
Disulfide bond167 ↔ 184 By similarity
Disulfide bond198 ↔ 210 By similarity
Disulfide bond205 ↔ 223 By similarity
Disulfide bond217 ↔ 232 By similarity
Disulfide bond237 ↔ 249 By similarity
Disulfide bond244 ↔ 262 By similarity
Disulfide bond256 ↔ 271 By similarity
Disulfide bond277 ↔ 290 By similarity
Disulfide bond285 ↔ 303 By similarity
Disulfide bond297 ↔ 314 By similarity
Disulfide bond319 ↔ 330 By similarity
Disulfide bond326 ↔ 339 By similarity
Disulfide bond341 ↔ 353 By similarity
Disulfide bond359 ↔ 369 By similarity
Disulfide bond365 ↔ 378 By similarity
Disulfide bond380 ↔ 393 By similarity
Disulfide bond667 ↔ 681 By similarity
Disulfide bond677 ↔ 696 By similarity
Disulfide bond698 ↔ 711 By similarity

Sequences

Sequence LengthMass (Da)Tools
P35952 [UniParc].

Last modified June 1, 1994. Version 1.
Checksum: E89F354E3F7C9005

FASTA87996,622
        10         20         30         40         50         60 
MSTADLMLRW AIALLLAAAG VAAEDSCGKN EFQCRDGKCI VSKWVCDGSR ECPDGSDESP 

        70         80         90        100        110        120 
ETCMSVTCRS GEFSCGGRVS RCIPDSWRCD GRTDCENGSD ELDCSPKTCS LDEFRCQDGK 

       130        140        150        160        170        180 
CISRQFVCDQ DWDCLDGSDE AHCAATTCGP AHFRCNSSSC IPSLWACDGD RDCDDGSDEW 

       190        200        210        220        230        240 
PQNCGAEDTA AEVVSSPCSS LEFHCGSSEC IHRSWVCDGA ADCKDKSDEE NCAVTTCRPD 

       250        260        270        280        290        300 
EFQCADGSCI HGSRQCDREH DCKDMSDELG CINVTQCDGP NKFKCHSGEC ISLDKVCNSA 

       310        320        330        340        350        360 
RDCRDWSDEP IKECKTNECL DNNGGCSHIC KDLKIGYECL CPSGFRLVDG HQCEDIDECQ 

       370        380        390        400        410        420 
EPDTCSQLCV NLEGSFKCEC RAGFHMDPHT RVCKAVGSIG FLLFTNRHEV RKMTLDRSEY 

       430        440        450        460        470        480 
TSLIPNLKNV VALDTEVANN RIYWSDLSQR KIYSAVMDQG TSLSYDAIIS GDLHAPDGLA 

       490        500        510        520        530        540 
VDWIHGNIYW TDSVPGTVSV ADTKGVRRRT LFREKGSRPR AIVVDPVHGF MYWTDWGTPA 

       550        560        570        580        590        600 
KIKKGGLNGV DIYSLVTEDI QWPNGITLDL PSGRLYWVDS KLHSISSIDV NGGGRKTILE 

       610        620        630        640        650        660 
DEKQLAHPFS LAIYEDKVYW TDVLNEAIFS ANRLTGSDVN LVAKNLMSPE DIVLFHNVTQ 

       670        680        690        700        710        720 
PRGVNWCEAT VLPNGGCQYM CLPAPQISAH SPKFTCACPD GMLLAKDMRS CLPEVDTVPT 

       730        740        750        760        770        780 
TQGTSTIGPV VTTSAAVSLK RKEDPSATRH KEDPSATRHN EDPSATSTSR QPGDTPELST 

       790        800        810        820        830        840 
VESVTVSSQV QGDMAGRGDE VQRHGVGFLS IFLPIALVAL LVFGAILLWR NWRLRNINSI 

       850        860        870 
NFDNPVYQKT TEDEIHICRS QDGYTYPSRQ MVSLEDDVA

« Hide

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		X13722 mRNA. Translation: CAA32001.1.
IPIIPI00203747.
PIRQRRTLD. S03430.
RefSeqNP_786938.1. NM_175762.2.
UniGeneRn.10483.

3D structure databases

ProteinModelPortalP35952.
SMRP35952. Positions 65-714.
ModBaseSearch...

Protein-protein interaction databases

STRING10116.ENSRNOP00000013496.

PTM databases

PhosphoSiteP35952.

Proteomic databases

PaxDbP35952.
PRIDEP35952.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

GeneID300438.
KEGGrno:300438.
UCSCRGD:2998. rat.

Organism-specific databases

CTD3949.
RGD2998. Ldlr.

Phylogenomic databases

eggNOGNOG255913.
HOGENOMHOG000115656.
HOVERGENHBG006250.
InParanoidP35952.
KOK12473.
OrthoDBEOG40S0F3.

Gene expression databases

ArrayExpressP35952.
GenevestigatorP35952.
GermOnlineENSRNOG00000009946. Rattus norvegicus.

Family and domain databases

Gene3D2.120.10.30. 1 hit.
4.10.400.10. 7 hits.
InterProIPR011042. 6-blade_b-propeller_TolB-like.
IPR026823. cEGF.
IPR000742. EG-like_dom.
IPR001881. EGF-like_Ca-bd.
IPR013032. EGF-like_CS.
IPR000152. EGF-type_Asp/Asn_hydroxyl_site.
IPR018097. EGF_Ca-bd_CS.
IPR009030. Growth_fac_rcpt.
IPR023415. LDLR_class-A_CS.
IPR000033. LDLR_classB_rpt.
IPR002172. LDrepeatLR_classA_rpt.
[Graphical view]
PfamPF12662. cEGF. 1 hit.
PF00057. Ldl_recept_a. 7 hits.
PF00058. Ldl_recept_b. 5 hits.
[Graphical view]
SMARTSM00181. EGF. 2 hits.
SM00179. EGF_CA. 1 hit.
SM00192. LDLa. 7 hits.
SM00135. LY. 5 hits.
[Graphical view]
SUPFAMSSF57184. Grow_fac_recept. 1 hit.
SSF57424. LDL_rcpt_classA_cys-rich. 7 hits.
PROSITEPS00010. ASX_HYDROXYL. 2 hits.
PS00022. EGF_1. False negative.
PS01186. EGF_2. 2 hits.
PS50026. EGF_3. 2 hits.
PS01187. EGF_CA. 1 hit.
PS01209. LDLRA_1. 7 hits.
PS50068. LDLRA_2. 7 hits.
PS51120. LDLRB. 5 hits.
[Graphical view]
ProtoNetSearch...

Other

NextBio646876.

Entry information

Entry nameLDLR_RAT
AccessionPrimary (citable) accession number: P35952
Entry history
Integrated into UniProtKB/Swiss-Prot: June 1, 1994
Last sequence update: June 1, 1994
Last modified: April 3, 2013
This is version 121 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents