Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Basket 0
(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

P35951

- LDLR_MOUSE

UniProt

P35951 - LDLR_MOUSE

Protein

Low-density lipoprotein receptor

Gene

Ldlr

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
    • BLAST
    • Align
    • Format
    • Add to basket
    • History
      Entry version 143 (01 Oct 2014)
      Sequence version 2 (06 Feb 2013)
      Previous versions | rss
    • Help video
    • Feedback
    • Comment

    Functioni

    Binds LDL, the major cholesterol-carrying lipoprotein of plasma, and transports it into cells by endocytosis. In order to be internalized, the receptor-ligand complexes must first cluster into clathrin-coated pits.

    GO - Molecular functioni

    1. calcium ion binding Source: InterPro
    2. low-density lipoprotein particle binding Source: MGI
    3. low-density lipoprotein receptor activity Source: MGI
    4. protein binding Source: MGI
    5. very-low-density lipoprotein particle receptor activity Source: MGI

    GO - Biological processi

    1. cholesterol homeostasis Source: MGI
    2. cholesterol import Source: BHF-UCL
    3. cholesterol metabolic process Source: MGI
    4. cholesterol transport Source: MGI
    5. intestinal cholesterol absorption Source: Ensembl
    6. lipid metabolic process Source: MGI
    7. lipoprotein catabolic process Source: MGI
    8. lipoprotein metabolic process Source: MGI
    9. low-density lipoprotein particle clearance Source: BHF-UCL
    10. phospholipid transport Source: BHF-UCL
    11. positive regulation of triglyceride biosynthetic process Source: BHF-UCL
    12. receptor-mediated endocytosis Source: GOC
    13. regulation of cholesterol homeostasis Source: MGI
    14. regulation of phosphatidylcholine catabolic process Source: BHF-UCL

    Keywords - Molecular functioni

    Receptor

    Keywords - Biological processi

    Cholesterol metabolism, Endocytosis, Lipid metabolism, Lipid transport, Steroid metabolism, Sterol metabolism, Transport

    Enzyme and pathway databases

    ReactomeiREACT_198569. Retinoid metabolism and transport.
    REACT_216017. Chylomicron-mediated lipid transport.
    REACT_222614. LDL-mediated lipid transport.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Low-density lipoprotein receptor
    Short name:
    LDL receptor
    Gene namesi
    Name:Ldlr
    OrganismiMus musculus (Mouse)
    Taxonomic identifieri10090 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
    ProteomesiUP000000589: Chromosome 9

    Organism-specific databases

    MGIiMGI:96765. Ldlr.

    Subcellular locationi

    Cell membrane; Single-pass type I membrane protein. Endomembrane system; Single-pass type I membrane protein. Membraneclathrin-coated pit By similarity; Single-pass type I membrane protein By similarity. Golgi apparatus By similarity. Early endosome By similarity. Late endosome By similarity. Cell surface By similarity. Lysosome By similarity
    Note: Found distributed from the plasma membrane to intracellular compartments. Localizes to the Golgi apparatus, early and late endosomes/lysosomes and cell surface in the presence of PCSK9 By similarity.By similarity

    GO - Cellular componenti

    1. cell surface Source: UniProtKB
    2. coated pit Source: UniProtKB-SubCell
    3. early endosome Source: UniProtKB
    4. endosome Source: MGI
    5. external side of plasma membrane Source: Ensembl
    6. Golgi apparatus Source: UniProtKB
    7. integral component of membrane Source: UniProtKB-KW
    8. late endosome Source: UniProtKB
    9. low-density lipoprotein particle Source: UniProtKB-KW
    10. lysosome Source: UniProtKB
    11. plasma membrane Source: Reactome
    12. receptor complex Source: MGI

    Keywords - Cellular componenti

    Cell membrane, Coated pit, Endosome, Golgi apparatus, LDL, Lysosome, Membrane

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Signal peptidei1 – 2121By similarityAdd
    BLAST
    Chaini22 – 862841Low-density lipoprotein receptorPRO_0000017313Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Disulfide bondi34 ↔ 52By similarity
    Disulfide bondi46 ↔ 63By similarity
    Disulfide bondi68 ↔ 82By similarity
    Disulfide bondi75 ↔ 95By similarity
    Disulfide bondi89 ↔ 104By similarity
    Glycosylationi97 – 971N-linked (GlcNAc...)Sequence Analysis
    Disulfide bondi109 ↔ 121By similarity
    Disulfide bondi116 ↔ 134By similarity
    Disulfide bondi128 ↔ 143By similarity
    Disulfide bondi148 ↔ 160By similarity
    Disulfide bondi155 ↔ 173By similarity
    Disulfide bondi167 ↔ 184By similarity
    Disulfide bondi198 ↔ 210By similarity
    Disulfide bondi205 ↔ 223By similarity
    Disulfide bondi217 ↔ 232By similarity
    Disulfide bondi237 ↔ 249By similarity
    Disulfide bondi244 ↔ 262By similarity
    Disulfide bondi256 ↔ 271By similarity
    Glycosylationi273 – 2731N-linked (GlcNAc...)Sequence Analysis
    Disulfide bondi277 ↔ 290By similarity
    Disulfide bondi285 ↔ 303By similarity
    Disulfide bondi297 ↔ 314By similarity
    Disulfide bondi319 ↔ 330By similarity
    Disulfide bondi326 ↔ 339By similarity
    Disulfide bondi341 ↔ 353By similarity
    Disulfide bondi359 ↔ 369By similarity
    Disulfide bondi365 ↔ 378By similarity
    Disulfide bondi380 ↔ 393By similarity
    Glycosylationi462 – 4621N-linked (GlcNAc...)Sequence Analysis
    Disulfide bondi667 ↔ 682By similarity
    Disulfide bondi678 ↔ 697By similarity
    Disulfide bondi699 ↔ 712By similarity
    Modified residuei725 – 7251PhosphothreonineBy similarity
    Modified residuei733 – 7331PhosphothreonineBy similarity
    Modified residuei735 – 7351PhosphoserineBy similarity

    Post-translational modificationi

    Ubiquitinated by MYLIP leading to degradation.By similarity

    Keywords - PTMi

    Disulfide bond, Glycoprotein, Phosphoprotein, Ubl conjugation

    Proteomic databases

    PaxDbiP35951.
    PRIDEiP35951.

    PTM databases

    PhosphoSiteiP35951.

    Expressioni

    Gene expression databases

    CleanExiMM_LDLR.
    GenevestigatoriP35951.

    Interactioni

    Subunit structurei

    Interacts with LDLRAP1, ARRB1, SNX17. Interacts (via NPXY motif) with DAB2 (via PID domain); the interaction is impaired by tyrosine phosphorylation of the NPXY motif. Interacts with the full length immature form of PCSK9 (via C-terminus).2 Publications

    Protein-protein interaction databases

    BioGridi201133. 2 interactions.
    IntActiP35951. 2 interactions.
    MINTiMINT-1541606.

    Structurei

    3D structure databases

    ProteinModelPortaliP35951.
    SMRiP35951. Positions 22-715.
    ModBaseiSearch...
    MobiDBiSearch...

    Topological domain

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Topological domaini22 – 790769ExtracellularSequence AnalysisAdd
    BLAST
    Topological domaini813 – 86250CytoplasmicSequence AnalysisAdd
    BLAST

    Transmembrane

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transmembranei791 – 81222HelicalSequence AnalysisAdd
    BLAST

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini25 – 6541LDL-receptor class A 1PROSITE-ProRule annotationAdd
    BLAST
    Domaini66 – 10641LDL-receptor class A 2PROSITE-ProRule annotationAdd
    BLAST
    Domaini107 – 14539LDL-receptor class A 3PROSITE-ProRule annotationAdd
    BLAST
    Domaini146 – 18641LDL-receptor class A 4PROSITE-ProRule annotationAdd
    BLAST
    Domaini196 – 23439LDL-receptor class A 5PROSITE-ProRule annotationAdd
    BLAST
    Domaini235 – 27339LDL-receptor class A 6PROSITE-ProRule annotationAdd
    BLAST
    Domaini275 – 31440LDL-receptor class A 7PROSITE-ProRule annotationAdd
    BLAST
    Domaini315 – 35440EGF-like 1PROSITE-ProRule annotationAdd
    BLAST
    Domaini355 – 39440EGF-like 2; calcium-bindingPROSITE-ProRule annotationAdd
    BLAST
    Repeati398 – 43942LDL-receptor class B 1Add
    BLAST
    Repeati440 – 48546LDL-receptor class B 2Add
    BLAST
    Repeati486 – 52843LDL-receptor class B 3Add
    BLAST
    Repeati529 – 57244LDL-receptor class B 4Add
    BLAST
    Repeati573 – 61543LDL-receptor class B 5Add
    BLAST
    Repeati616 – 65843LDL-receptor class B 6Add
    BLAST
    Domaini663 – 71351EGF-like 3PROSITE-ProRule annotationAdd
    BLAST

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni722 – 77049Clustered O-linked oligosaccharidesAdd
    BLAST
    Regioni813 – 86250Required for MYLIP-triggered down-regulation of LDLRBy similarityAdd
    BLAST

    Motif

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Motifi825 – 8306NPXY motif

    Domaini

    The NPXY motif mediates the interaction with the clathrin adaptor DAB2 involved in receptor internalization.1 Publication

    Sequence similaritiesi

    Belongs to the LDLR family.Curated
    Contains 3 EGF-like domains.PROSITE-ProRule annotation
    Contains 7 LDL-receptor class A domains.PROSITE-ProRule annotation
    Contains 6 LDL-receptor class B repeats.PROSITE-ProRule annotation

    Keywords - Domaini

    EGF-like domain, Repeat, Signal, Transmembrane, Transmembrane helix

    Phylogenomic databases

    eggNOGiNOG255913.
    GeneTreeiENSGT00740000114992.
    HOGENOMiHOG000115656.
    HOVERGENiHBG006250.
    InParanoidiP35951.
    KOiK12473.
    OMAiGQVDCEN.
    OrthoDBiEOG7NGQ9P.
    TreeFamiTF351700.

    Family and domain databases

    Gene3Di2.120.10.30. 1 hit.
    4.10.400.10. 7 hits.
    InterProiIPR011042. 6-blade_b-propeller_TolB-like.
    IPR000742. EG-like_dom.
    IPR001881. EGF-like_Ca-bd_dom.
    IPR013032. EGF-like_CS.
    IPR000152. EGF-type_Asp/Asn_hydroxyl_site.
    IPR018097. EGF_Ca-bd_CS.
    IPR009030. Growth_fac_rcpt_N_dom.
    IPR023415. LDLR_class-A_CS.
    IPR000033. LDLR_classB_rpt.
    IPR002172. LDrepeatLR_classA_rpt.
    [Graphical view]
    PfamiPF07645. EGF_CA. 1 hit.
    PF00057. Ldl_recept_a. 7 hits.
    PF00058. Ldl_recept_b. 5 hits.
    [Graphical view]
    PRINTSiPR00261. LDLRECEPTOR.
    SMARTiSM00181. EGF. 2 hits.
    SM00179. EGF_CA. 1 hit.
    SM00192. LDLa. 7 hits.
    SM00135. LY. 5 hits.
    [Graphical view]
    SUPFAMiSSF57184. SSF57184. 1 hit.
    SSF57424. SSF57424. 6 hits.
    PROSITEiPS00010. ASX_HYDROXYL. 1 hit.
    PS01186. EGF_2. 2 hits.
    PS50026. EGF_3. 2 hits.
    PS01187. EGF_CA. 1 hit.
    PS01209. LDLRA_1. 7 hits.
    PS50068. LDLRA_2. 7 hits.
    PS51120. LDLRB. 5 hits.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    P35951-1 [UniParc]FASTAAdd to Basket

    « Hide

    MSTADLMRRW VIALLLAAAG VAAEDSCSRN EFQCRDGKCI ASKWVCDGSP    50
    ECPDGSDESP ETCMSVTCQS NQFSCGGRVS RCIPDSWRCD GQVDCENDSD 100
    EQGCPPKTCS QDDFRCQDGK CISPQFVCDG DRDCLDGSDE AHCQATTCGP 150
    AHFRCNSSIC IPSLWACDGD VDCVDGSDEW PQNCQGRDTA SKGVSSPCSS 200
    LEFHCGSSEC IHRSWVCDGE ADCKDKSDEE HCAVATCRPD EFQCADGSCI 250
    HGSRQCDREH DCKDMSDELG CVNVTQCDGP NKFKCHSGEC ISLDKVCDSA 300
    RDCQDWSDEP IKECKTNECL DNNGGCSHIC KDLKIGSECL CPSGFRLVDL 350
    HRCEDIDECQ EPDTCSQLCV NLEGSYKCEC QAGFHMDPHT RVCKAVGSIG 400
    YLLFTNRHEV RKMTLDRSEY TSLLPNLKNV VALDTEVTNN RIYWSDLSQK 450
    KIYSALMDQA PNLSYDTIIS EDLHAPDGLA VDWIHRNIYW TDSVPGSVSV 500
    ADTKGVKRRT LFQEAGSRPR AIVVDPVHGF MYWTDWGTPA KIKKGGLNGV 550
    DIHSLVTENI QWPNGITLDL SSGRLYWVDS KLHSISSIDV NGGNRKTILE 600
    DENRLAHPFS LAIYEDKVYW TDVINEAIFS ANRLTGSDVN LVAENLLSPE 650
    DIVLFHKVTQ PRGVNWCETT ALLPNGGCQY LCLPAPQIGP HSPKFTCACP 700
    DGMLLAKDMR SCLTEVDTVL TTQGTSAVRP VVTASATRPP KHSEDLSAPS 750
    TPRQPVDTPG LSTVASVTVS HQVQGDMAGR GNEEQPHGMR FLSIFFPIAL 800
    VALLVLGAVL LWRNWRLKNI NSINFDNPVY QKTTEDELHI CRSQDGYTYP 850
    SRQMVSLEDD VA 862
    Length:862
    Mass (Da):94,947
    Last modified:February 6, 2013 - v2
    Checksum:i43E7D6A293BE609C
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti23 – 231A → V in CAA79581. (PubMed:8466528)Curated
    Sequence conflicti27 – 271C → G in CAA79581. (PubMed:8466528)Curated
    Sequence conflicti61 – 611E → K in CAA79581. (PubMed:8466528)Curated
    Sequence conflicti144 – 1441Q → P in CAA79581. (PubMed:8466528)Curated
    Sequence conflicti156 – 1561N → K in CAA79581. (PubMed:8466528)Curated
    Sequence conflicti178 – 1781D → H in CAA79581. (PubMed:8466528)Curated
    Sequence conflicti186 – 1872GR → AE in CAA79581. (PubMed:8466528)Curated
    Sequence conflicti819 – 8191N → NIT in CAA79581. (PubMed:8466528)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    Z19521 mRNA. Translation: CAA79581.1.
    X64414 mRNA. Translation: CAA45759.1.
    AC161371 Genomic DNA. No translation available.
    CH466522 Genomic DNA. Translation: EDL25212.1.
    BC053041 mRNA. Translation: AAH53041.1.
    CCDSiCCDS22910.1.
    PIRiI48623. QRMSLD.
    RefSeqiNP_001239587.1. NM_001252658.1.
    NP_001239588.1. NM_001252659.1.
    NP_034830.2. NM_010700.3.
    UniGeneiMm.3213.

    Genome annotation databases

    EnsembliENSMUST00000034713; ENSMUSP00000034713; ENSMUSG00000032193.
    GeneIDi16835.
    KEGGimmu:16835.
    UCSCiuc009omi.2. mouse.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    Z19521 mRNA. Translation: CAA79581.1 .
    X64414 mRNA. Translation: CAA45759.1 .
    AC161371 Genomic DNA. No translation available.
    CH466522 Genomic DNA. Translation: EDL25212.1 .
    BC053041 mRNA. Translation: AAH53041.1 .
    CCDSi CCDS22910.1.
    PIRi I48623. QRMSLD.
    RefSeqi NP_001239587.1. NM_001252658.1.
    NP_001239588.1. NM_001252659.1.
    NP_034830.2. NM_010700.3.
    UniGenei Mm.3213.

    3D structure databases

    ProteinModelPortali P35951.
    SMRi P35951. Positions 22-715.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 201133. 2 interactions.
    IntActi P35951. 2 interactions.
    MINTi MINT-1541606.

    PTM databases

    PhosphoSitei P35951.

    Proteomic databases

    PaxDbi P35951.
    PRIDEi P35951.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSMUST00000034713 ; ENSMUSP00000034713 ; ENSMUSG00000032193 .
    GeneIDi 16835.
    KEGGi mmu:16835.
    UCSCi uc009omi.2. mouse.

    Organism-specific databases

    CTDi 3949.
    MGIi MGI:96765. Ldlr.

    Phylogenomic databases

    eggNOGi NOG255913.
    GeneTreei ENSGT00740000114992.
    HOGENOMi HOG000115656.
    HOVERGENi HBG006250.
    InParanoidi P35951.
    KOi K12473.
    OMAi GQVDCEN.
    OrthoDBi EOG7NGQ9P.
    TreeFami TF351700.

    Enzyme and pathway databases

    Reactomei REACT_198569. Retinoid metabolism and transport.
    REACT_216017. Chylomicron-mediated lipid transport.
    REACT_222614. LDL-mediated lipid transport.

    Miscellaneous databases

    NextBioi 290754.
    PROi P35951.
    SOURCEi Search...

    Gene expression databases

    CleanExi MM_LDLR.
    Genevestigatori P35951.

    Family and domain databases

    Gene3Di 2.120.10.30. 1 hit.
    4.10.400.10. 7 hits.
    InterProi IPR011042. 6-blade_b-propeller_TolB-like.
    IPR000742. EG-like_dom.
    IPR001881. EGF-like_Ca-bd_dom.
    IPR013032. EGF-like_CS.
    IPR000152. EGF-type_Asp/Asn_hydroxyl_site.
    IPR018097. EGF_Ca-bd_CS.
    IPR009030. Growth_fac_rcpt_N_dom.
    IPR023415. LDLR_class-A_CS.
    IPR000033. LDLR_classB_rpt.
    IPR002172. LDrepeatLR_classA_rpt.
    [Graphical view ]
    Pfami PF07645. EGF_CA. 1 hit.
    PF00057. Ldl_recept_a. 7 hits.
    PF00058. Ldl_recept_b. 5 hits.
    [Graphical view ]
    PRINTSi PR00261. LDLRECEPTOR.
    SMARTi SM00181. EGF. 2 hits.
    SM00179. EGF_CA. 1 hit.
    SM00192. LDLa. 7 hits.
    SM00135. LY. 5 hits.
    [Graphical view ]
    SUPFAMi SSF57184. SSF57184. 1 hit.
    SSF57424. SSF57424. 6 hits.
    PROSITEi PS00010. ASX_HYDROXYL. 1 hit.
    PS01186. EGF_2. 2 hits.
    PS50026. EGF_3. 2 hits.
    PS01187. EGF_CA. 1 hit.
    PS01209. LDLRA_1. 7 hits.
    PS50068. LDLRA_2. 7 hits.
    PS51120. LDLRB. 5 hits.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [MRNA].
      Strain: BALB/c.
      Tissue: Liver.
    2. "Molecular cloning and nucleotide sequence of cDNA encoding a functional murine low-density-lipoprotein receptor."
      Polvino W.J., Dichek D.A., Mason J., Anderson W.F.
      Somat. Cell Mol. Genet. 18:443-450(1992) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
      Tissue: Liver.
    3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: C57BL/6J.
    4. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Strain: C57BL/6.
      Tissue: Brain.
    5. Mural R.J., Adams M.D., Myers E.W., Smith H.O., Venter J.C.
      Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    6. "Disabled-2 colocalizes with the LDLR in clathrin-coated pits and interacts with AP-2."
      Morris S.M., Cooper J.A.
      Traffic 2:111-123(2001) [PubMed] [Europe PMC] [Abstract]
      Cited for: INTERACTION WITH DAB2, DOMAIN.
    7. "The PX-domain protein SNX17 interacts with members of the LDL receptor family and modulates endocytosis of the LDL receptor."
      Stockinger W., Sailler B., Strasser V., Recheis B., Fasching D., Kahr L., Schneider W.J., Nimpf J.
      EMBO J. 21:4259-4267(2002) [PubMed] [Europe PMC] [Abstract]
      Cited for: INTERACTION WITH SNX17.

    Entry informationi

    Entry nameiLDLR_MOUSE
    AccessioniPrimary (citable) accession number: P35951
    Secondary accession number(s): Q6GTJ9
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: June 1, 1994
    Last sequence update: February 6, 2013
    Last modified: October 1, 2014
    This is version 143 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. MGD cross-references
      Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3