Reviewed,
UniProtKB/Swiss-Prot P35946 (L_PHODV)
Last modified
January 19, 2010.
Version 44.
History...
Clusters with 100%,
90%,
50% identity |
 Documents (1) |
 Third-party data |
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Names and origin
| Protein names | Recommended name: Large structural protein Short name=Protein L Alternative name(s): Transcriptase Replicase Including the following 3 domains: 1- Recommended name: RNA-directed RNA polymerase EC=2.7.7.48 2- Recommended name: mRNA (guanine-N(7)-)-methyltransferase EC=2.1.1.56 3- Recommended name: mRNA guanylyltransferase EC=2.7.7.- | ||
| Gene names |
| ||
| Organism | Phocine distemper virus (PDV) | ||
| Taxonomic identifier | 11240 [NCBI] | ||
| Taxonomic lineage | Viruses › ssRNA negative-strand viruses › Mononegavirales › Paramyxoviridae › Paramyxovirinae › Morbillivirus | ||
| Virus host | Phocidae (true seals) [TaxID: 9709] |
Protein attributes
| Sequence length | 10 AA. |
| Sequence status | Fragment. |
| Protein existence | Inferred from homology. |
General annotation (Comments)
| Function | Displays RNA-directed RNA polymerase, mRNA guanylyl transferase, mRNA (guanine-N(7)-)-methyltransferase and poly(A) synthetase activities. The viral mRNA guanylyl transferase displays a different biochemical reaction than the cellular enzyme. The template is composed of the viral RNA tightly encapsidated by the nucleoprotein (N). Functions either as transcriptase or as replicase. The transcriptase synthesizes subsequently the subgenomic RNAs, assuring their capping and polyadenylation by a stuttering mechanism. The transcriptase stutters on a specific sequence, resulting on a cotranscriptional editing of the phosphoprotein (P) mRNA. The replicase mode is dependent on intracellular N protein concentration. In this mode, the polymerase replicates the whole viral genome without recognizing the transcriptional signals By similarity. |
| Catalytic activity | Nucleoside triphosphate + RNA(n) = diphosphate + RNA(n+1). S-adenosyl-L-methionine + G(5')pppR-RNA = S-adenosyl-L-homocysteine + m7G(5')pppR-RNA. |
| Subunit structure | Interacts with the P protein By similarity. |
| Subcellular location | Virion Potential. Host cytoplasm By similarity. |
| Sequence similarities | Belongs to the paramyxoviruses L protein family. |
Ontologies
| Keywords | |
|---|---|
| Biological process | RNA replication mRNA capping mRNA processing |
| Cellular component | Host cytoplasm Virion |
| Ligand | ATP-binding Nucleotide-binding S-adenosyl-L-methionine |
| Molecular function | Methyltransferase Nucleotidyltransferase RNA-directed RNA polymerase Transferase |
| Technical term | Multifunctional enzyme |
| Gene Ontology (GO) | |
| Biological process | mRNA capping Inferred from electronic annotation. Source: UniProtKB-KW transcription, RNA-dependentInferred from electronic annotation. Source: UniProtKB-KW |
| Cellular component | host cell cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell virionInferred from electronic annotation. Source: UniProtKB-SubCell |
| Molecular function | ATP binding Inferred from electronic annotation. Source: UniProtKB-KW RNA-directed RNA polymerase activityInferred from electronic annotation. Source: UniProtKB-KW mRNA (guanine-N7-)-methyltransferase activityInferred from electronic annotation. Source: EC |
| Complete GO annotation... | |
Sequence annotation (Features)
Sequences
References
| [1] | "Molecular characterization of phocine distemper virus: gene order and sequence of the gene encoding the attachment (H) protein." Curran M.D., O'Loan D., Kennedy S., Rima B.K. J. Gen. Virol. 73:1189-1194(1992) [PubMed: 1588321] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA]. Strain: Ulster/88. |
Cross-references
Entry information
| Entry name | L_PHODV | ||||||||
| Accession | Primary (citable) accession number: P35946 | ||||||||
| Entry history |
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| Entry status | Reviewed (UniProtKB/Swiss-Prot) | ||||||||
| Annotation project | Virus (Virus annotation project) | ||||||||
