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P35942

- RDRP_ROTGI

UniProt

P35942 - RDRP_ROTGI

Protein

RNA-directed RNA polymerase

Gene
N/A
Organism
Rotavirus B (isolate Rat/United States/IDIR/1984) (RV-B) (Rotavirus B (isolate infectious diarrhea of infant rats))
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
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    • History
      Entry version 59 (01 Oct 2014)
      Sequence version 1 (01 Jun 1994)
      Previous versions | rss
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    Functioni

    RNA-directed RNA polymerase that is involved in both transcription and genome replication. Together with VP3 capping enzyme, forms an enzyme complex positioned near the channels situated at each of the five-fold vertices of the core. Following infection, the outermost layer of the virus is lost, leaving a double-layered particle (DLP) made up of the core and VP6 shell. VP1 then catalyzes the transcription of fully conservative plus-strand genomic RNAs that are extruded through the DLP's channels into the cytoplasm where they function as mRNAs for translation of viral proteins. One copy of each of the viral (+)RNAs is also recruited during core assembly, together with newly synthesized polymerase complexes and VP2. The polymerase of these novo-formed particles catalyzes the synthesis of complementary minus-strands leading to dsDNA formation. To do so, the polymerase specifically recognizes conserved 3' sequence(s) in plus-strand RNA templates. Once dsRNA synthesis is complete, the polymerase switches to the transcriptional mode, thus providing secondary transcription By similarity.PROSITE-ProRule annotation

    Catalytic activityi

    Nucleoside triphosphate + RNA(n) = diphosphate + RNA(n+1).PROSITE-ProRule annotation

    GO - Molecular functioni

    1. nucleotide binding Source: UniProtKB-KW
    2. RNA binding Source: UniProtKB-KW
    3. RNA-directed RNA polymerase activity Source: UniProtKB-KW

    GO - Biological processi

    1. transcription, DNA-templated Source: InterPro
    2. viral genome replication Source: InterPro

    Keywords - Molecular functioni

    Nucleotidyltransferase, RNA-directed RNA polymerase, Transferase

    Keywords - Biological processi

    Viral RNA replication

    Keywords - Ligandi

    Nucleotide-binding, RNA-binding

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    RNA-directed RNA polymerase (EC:2.7.7.48)
    Alternative name(s):
    Protein VP1
    OrganismiRotavirus B (isolate Rat/United States/IDIR/1984) (RV-B) (Rotavirus B (isolate infectious diarrhea of infant rats))
    Taxonomic identifieri28877 [NCBI]
    Taxonomic lineageiVirusesdsRNA virusesReoviridaeSedoreovirinaeRotavirus
    Virus hostiHomo sapiens (Human) [TaxID: 9606]
    Rattus norvegicus (Rat) [TaxID: 10116]

    Subcellular locationi

    Virion Curated
    Note: Attached inside the inner capsid as a minor component. Also found in spherical cytoplasmic structures, called virus factories, that appear early after infection and are the site of viral replication and packaging By similarity.By similarity

    GO - Cellular componenti

    1. virion Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Virion

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 11591159RNA-directed RNA polymerasePRO_0000149530Add
    BLAST

    Interactioni

    Subunit structurei

    Interacts with VP3 Potential. Interacts with VP2 Potential. Interacts with NSP5; this interaction is probably necessary for the formation of functional virus factories By similarity.By similarityCurated

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini545 – 727183RdRp catalyticPROSITE-ProRule annotationAdd
    BLAST

    Sequence similaritiesi

    Contains 1 RdRp catalytic domain.PROSITE-ProRule annotation

    Family and domain databases

    InterProiIPR001795. RNA-dir_pol_luteovirus.
    IPR007097. RNA-dir_pol_reovirus.
    [Graphical view]
    PfamiPF02123. RdRP_4. 1 hit.
    [Graphical view]
    PROSITEiPS50523. RDRP_DSRNA_REO. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P35942-1 [UniParc]FASTAAdd to Basket

    « Hide

    MDSFQFFEWL LRDIERNLLY TSLVYTNPKI AIVRYEPSEN AKLWRSKELN     50
    TDSPNELLLT LKKTLDECST LDEKIETLLR IRYFTVYVGD KSDKRNIIRS 100
    WLSTTINRLN VEEYASIQEI ELQAKQWRAE NAKSLRPYHY NIPINEYLRD 150
    NEIEVLDTGD NRWKSDTLQG LLPNFYHRTH TLIGAILFAV TSRLEIYSYE 200
    QKKALRYLLR TIEKCYNEGY LEMSRDRKWS HTLPELRTLT FRLYNSKVIH 250
    AACAMISLVH ANPIDYEFLC QVVAVYQIIP ANAAKLLSTP MTLYVGVATF 300
    SSNQVASTGN ASECAPLQIE NNIYVSEAQK KEWAESFKSD PLNKSRMLKL 350
    MNENLNTTLD KFSLIFNCFS STFHVGHRID NAQDAITDQV TATYTSNVNR 400
    EMYDSYYYKL KQMLKEEIVQ YVEDHVAKQY KDVTAESLSA LANSSNGFSK 450
    EVKFIDRNIK TTKKILHLDN DLITNDYSDL SKALSHGIPM GTRNVPARQT 500
    RGIFILPWQV AAVQHTIAES LYKRAKKGAY QGSFAEAYTA KTASLTYGVL 550
    AEDTSKATKI ILYTDVSQWD ASQHNTEPYR SAWINAIREA RAELKWRYDD 600
    EPKVLEMNVL DSMIKIQEYL LNSNLVVASP GSQRPTKIIR YHGVASGEKT 650
    TKIGNSFANV ALIETVLDFA KTDIPDLEIS HLRVDGDDNV VSINTSCTID 700
    RLQRIIKEKY SSLNARVKAL ASYTGLEMAK RFIICGKIFE RGAIPIFTAE 750
    RPYGTDVSIQ SMCGSSIYSS AVNAYRGFGD AYFSFMQDVL VPPSASTRIT 800
    GRLRVLMSPV TLYATGPLSF EITPQGLGGR CRFFTQSAKL FTLFKMLTQT 850
    AAVSITPDEI KKYAETVQFK KRTEVMIASM QRKLLVPAKA LARIIVDKEQ 900
    QKTLGVPNVQ SQKNRSQVSK AIEILGVPER NDIVAKGYYP EELYSLIISH 950
    SVVVYTSHST PISIYRVNCE PVELLRSQLG IRIADSKPIA KPTNHLYDIV 1000
    SGLSPIKISP SDLLTQAKKY DLSTYKGKRD YLSDLGLVGN TLKTYLASKM 1050
    LFRDLLMSKY DDLYSTPGFG ATQLTTIPLD VTSAEKVFSI RLGLPPHLYE 1100
    VVMLLLLYEY IHYVFSCKRT FTAQMHAISQ EQSAVITKNI ILMLDNIQLD 1150
    QVSFSDDAW 1159
    Length:1,159
    Mass (Da):131,650
    Last modified:June 1, 1994 - v1
    Checksum:iE355F9BF79E225A8
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M97203 Genomic RNA. No translation available.
    PIRiA44280.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M97203 Genomic RNA. No translation available.
    PIRi A44280.

    3D structure databases

    ModBasei Search...
    MobiDBi Search...

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Family and domain databases

    InterProi IPR001795. RNA-dir_pol_luteovirus.
    IPR007097. RNA-dir_pol_reovirus.
    [Graphical view ]
    Pfami PF02123. RdRP_4. 1 hit.
    [Graphical view ]
    PROSITEi PS50523. RDRP_DSRNA_REO. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Sequence analysis of group B rotavirus gene 1 and definition of a rotavirus-specific sequence motif within the RNA polymerase gene."
      Eiden J.J., Hirshon C.
      Virology 192:154-160(1993) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA].

    Entry informationi

    Entry nameiRDRP_ROTGI
    AccessioniPrimary (citable) accession number: P35942
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: June 1, 1994
    Last sequence update: June 1, 1994
    Last modified: October 1, 2014
    This is version 59 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programViral Protein Annotation Program

    Miscellaneousi

    Documents

    1. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3