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P35942 (RDRP_ROTGI) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 58. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
RNA-directed RNA polymerase

EC=2.7.7.48
Alternative name(s):
Protein VP1
OrganismRotavirus B (isolate Rat/United States/IDIR/1984) (RV-B) (Rotavirus B (isolate infectious diarrhea of infant rats))
Taxonomic identifier28877 [NCBI]
Taxonomic lineageVirusesdsRNA virusesReoviridaeSedoreovirinaeRotavirus
Virus hostHomo sapiens (Human) [TaxID: 9606]
Rattus norvegicus (Rat) [TaxID: 10116]

Protein attributes

Sequence length1159 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

RNA-directed RNA polymerase that is involved in both transcription and genome replication. Together with VP3 capping enzyme, forms an enzyme complex positioned near the channels situated at each of the five-fold vertices of the core. Following infection, the outermost layer of the virus is lost, leaving a double-layered particle (DLP) made up of the core and VP6 shell. VP1 then catalyzes the transcription of fully conservative plus-strand genomic RNAs that are extruded through the DLP's channels into the cytoplasm where they function as mRNAs for translation of viral proteins. One copy of each of the viral (+)RNAs is also recruited during core assembly, together with newly synthesized polymerase complexes and VP2. The polymerase of these novo-formed particles catalyzes the synthesis of complementary minus-strands leading to dsDNA formation. To do so, the polymerase specifically recognizes conserved 3' sequence(s) in plus-strand RNA templates. Once dsRNA synthesis is complete, the polymerase switches to the transcriptional mode, thus providing secondary transcription By similarity.

Catalytic activity

Nucleoside triphosphate + RNA(n) = diphosphate + RNA(n+1).

Subunit structure

Interacts with VP3 Potential. Interacts with VP2 Potential. Interacts with NSP5; this interaction is probably necessary for the formation of functional virus factories By similarity.

Subcellular location

Virion Potential. Note: Attached inside the inner capsid as a minor component. Also found in spherical cytoplasmic structures, called virus factories, that appear early after infection and are the site of viral replication and packaging By similarity.

Sequence similarities

Belongs to the reoviridae RNA-directed RNA polymerase family.

Contains 1 RdRp catalytic domain.

Ontologies

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 11591159RNA-directed RNA polymerase
PRO_0000149530

Regions

Domain545 – 727183RdRp catalytic

Sequences

Sequence LengthMass (Da)Tools
P35942 [UniParc].

Last modified June 1, 1994. Version 1.
Checksum: E355F9BF79E225A8

FASTA1,159131,650
        10         20         30         40         50         60 
MDSFQFFEWL LRDIERNLLY TSLVYTNPKI AIVRYEPSEN AKLWRSKELN TDSPNELLLT 

        70         80         90        100        110        120 
LKKTLDECST LDEKIETLLR IRYFTVYVGD KSDKRNIIRS WLSTTINRLN VEEYASIQEI 

       130        140        150        160        170        180 
ELQAKQWRAE NAKSLRPYHY NIPINEYLRD NEIEVLDTGD NRWKSDTLQG LLPNFYHRTH 

       190        200        210        220        230        240 
TLIGAILFAV TSRLEIYSYE QKKALRYLLR TIEKCYNEGY LEMSRDRKWS HTLPELRTLT 

       250        260        270        280        290        300 
FRLYNSKVIH AACAMISLVH ANPIDYEFLC QVVAVYQIIP ANAAKLLSTP MTLYVGVATF 

       310        320        330        340        350        360 
SSNQVASTGN ASECAPLQIE NNIYVSEAQK KEWAESFKSD PLNKSRMLKL MNENLNTTLD 

       370        380        390        400        410        420 
KFSLIFNCFS STFHVGHRID NAQDAITDQV TATYTSNVNR EMYDSYYYKL KQMLKEEIVQ 

       430        440        450        460        470        480 
YVEDHVAKQY KDVTAESLSA LANSSNGFSK EVKFIDRNIK TTKKILHLDN DLITNDYSDL 

       490        500        510        520        530        540 
SKALSHGIPM GTRNVPARQT RGIFILPWQV AAVQHTIAES LYKRAKKGAY QGSFAEAYTA 

       550        560        570        580        590        600 
KTASLTYGVL AEDTSKATKI ILYTDVSQWD ASQHNTEPYR SAWINAIREA RAELKWRYDD 

       610        620        630        640        650        660 
EPKVLEMNVL DSMIKIQEYL LNSNLVVASP GSQRPTKIIR YHGVASGEKT TKIGNSFANV 

       670        680        690        700        710        720 
ALIETVLDFA KTDIPDLEIS HLRVDGDDNV VSINTSCTID RLQRIIKEKY SSLNARVKAL 

       730        740        750        760        770        780 
ASYTGLEMAK RFIICGKIFE RGAIPIFTAE RPYGTDVSIQ SMCGSSIYSS AVNAYRGFGD 

       790        800        810        820        830        840 
AYFSFMQDVL VPPSASTRIT GRLRVLMSPV TLYATGPLSF EITPQGLGGR CRFFTQSAKL 

       850        860        870        880        890        900 
FTLFKMLTQT AAVSITPDEI KKYAETVQFK KRTEVMIASM QRKLLVPAKA LARIIVDKEQ 

       910        920        930        940        950        960 
QKTLGVPNVQ SQKNRSQVSK AIEILGVPER NDIVAKGYYP EELYSLIISH SVVVYTSHST 

       970        980        990       1000       1010       1020 
PISIYRVNCE PVELLRSQLG IRIADSKPIA KPTNHLYDIV SGLSPIKISP SDLLTQAKKY 

      1030       1040       1050       1060       1070       1080 
DLSTYKGKRD YLSDLGLVGN TLKTYLASKM LFRDLLMSKY DDLYSTPGFG ATQLTTIPLD 

      1090       1100       1110       1120       1130       1140 
VTSAEKVFSI RLGLPPHLYE VVMLLLLYEY IHYVFSCKRT FTAQMHAISQ EQSAVITKNI 

      1150 
ILMLDNIQLD QVSFSDDAW 

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References

[1]"Sequence analysis of group B rotavirus gene 1 and definition of a rotavirus-specific sequence motif within the RNA polymerase gene."
Eiden J.J., Hirshon C.
Virology 192:154-160(1993) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA].

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
M97203 Genomic RNA. No translation available.
PIRA44280.

3D structure databases

ModBaseSearch...
MobiDBSearch...

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Family and domain databases

InterProIPR001795. RNA-dir_pol_luteovirus.
IPR007097. RNA-dir_pol_reovirus.
[Graphical view]
PfamPF02123. RdRP_4. 1 hit.
[Graphical view]
PROSITEPS50523. RDRP_DSRNA_REO. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameRDRP_ROTGI
AccessionPrimary (citable) accession number: P35942
Entry history
Integrated into UniProtKB/Swiss-Prot: June 1, 1994
Last sequence update: June 1, 1994
Last modified: April 16, 2014
This is version 58 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programViral Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families