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P35942

- RDRP_ROTGI

UniProt

P35942 - RDRP_ROTGI

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Protein

RNA-directed RNA polymerase

Gene
N/A
Organism
Rotavirus B (isolate Rat/United States/IDIR/1984) (RV-B) (Rotavirus B (isolate infectious diarrhea of infant rats))
Status
Reviewed - Annotation score: 3 out of 5 - Protein inferred from homologyi

Functioni

RNA-directed RNA polymerase that is involved in both transcription and genome replication. Together with VP3 capping enzyme, forms an enzyme complex positioned near the channels situated at each of the five-fold vertices of the core. Following infection, the outermost layer of the virus is lost, leaving a double-layered particle (DLP) made up of the core and VP6 shell. VP1 then catalyzes the transcription of fully conservative plus-strand genomic RNAs that are extruded through the DLP's channels into the cytoplasm where they function as mRNAs for translation of viral proteins. One copy of each of the viral (+)RNAs is also recruited during core assembly, together with newly synthesized polymerase complexes and VP2. The polymerase of these novo-formed particles catalyzes the synthesis of complementary minus-strands leading to dsDNA formation. To do so, the polymerase specifically recognizes conserved 3' sequence(s) in plus-strand RNA templates. Once dsRNA synthesis is complete, the polymerase switches to the transcriptional mode, thus providing secondary transcription By similarity.

Catalytic activityi

Nucleoside triphosphate + RNA(n) = diphosphate + RNA(n+1).

GO - Molecular functioni

  1. nucleotide binding Source: UniProtKB-KW
  2. RNA binding Source: UniProtKB-KW
  3. RNA-directed RNA polymerase activity Source: UniProtKB-KW

GO - Biological processi

  1. transcription, DNA-templated Source: InterPro
  2. viral genome replication Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Nucleotidyltransferase, RNA-directed RNA polymerase, Transferase

Keywords - Biological processi

Viral RNA replication

Keywords - Ligandi

Nucleotide-binding, RNA-binding

Names & Taxonomyi

Protein namesi
Recommended name:
RNA-directed RNA polymerase (EC:2.7.7.48)
Alternative name(s):
Protein VP1
OrganismiRotavirus B (isolate Rat/United States/IDIR/1984) (RV-B) (Rotavirus B (isolate infectious diarrhea of infant rats))
Taxonomic identifieri28877 [NCBI]
Taxonomic lineageiVirusesdsRNA virusesReoviridaeSedoreovirinaeRotavirus
Virus hostiHomo sapiens (Human) [TaxID: 9606]
Rattus norvegicus (Rat) [TaxID: 10116]

Subcellular locationi

Virion Reviewed prediction
Note: Attached inside the inner capsid as a minor component. Also found in spherical cytoplasmic structures, called virus factories, that appear early after infection and are the site of viral replication and packaging By similarity.

GO - Cellular componenti

  1. virion Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Virion

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 11591159RNA-directed RNA polymerasePRO_0000149530Add
BLAST

Interactioni

Subunit structurei

Interacts with VP3 Reviewed prediction. Interacts with VP2 Reviewed prediction. Interacts with NSP5; this interaction is probably necessary for the formation of functional virus factories By similarity.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini545 – 727183RdRp catalyticAdd
BLAST

Sequence similaritiesi

Family and domain databases

InterProiIPR001795. RNA-dir_pol_luteovirus.
IPR007097. RNA-dir_pol_reovirus.
[Graphical view]
PfamiPF02123. RdRP_4. 1 hit.
[Graphical view]
PROSITEiPS50523. RDRP_DSRNA_REO. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P35942-1 [UniParc]FASTAAdd to Basket

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MDSFQFFEWL LRDIERNLLY TSLVYTNPKI AIVRYEPSEN AKLWRSKELN     50
TDSPNELLLT LKKTLDECST LDEKIETLLR IRYFTVYVGD KSDKRNIIRS 100
WLSTTINRLN VEEYASIQEI ELQAKQWRAE NAKSLRPYHY NIPINEYLRD 150
NEIEVLDTGD NRWKSDTLQG LLPNFYHRTH TLIGAILFAV TSRLEIYSYE 200
QKKALRYLLR TIEKCYNEGY LEMSRDRKWS HTLPELRTLT FRLYNSKVIH 250
AACAMISLVH ANPIDYEFLC QVVAVYQIIP ANAAKLLSTP MTLYVGVATF 300
SSNQVASTGN ASECAPLQIE NNIYVSEAQK KEWAESFKSD PLNKSRMLKL 350
MNENLNTTLD KFSLIFNCFS STFHVGHRID NAQDAITDQV TATYTSNVNR 400
EMYDSYYYKL KQMLKEEIVQ YVEDHVAKQY KDVTAESLSA LANSSNGFSK 450
EVKFIDRNIK TTKKILHLDN DLITNDYSDL SKALSHGIPM GTRNVPARQT 500
RGIFILPWQV AAVQHTIAES LYKRAKKGAY QGSFAEAYTA KTASLTYGVL 550
AEDTSKATKI ILYTDVSQWD ASQHNTEPYR SAWINAIREA RAELKWRYDD 600
EPKVLEMNVL DSMIKIQEYL LNSNLVVASP GSQRPTKIIR YHGVASGEKT 650
TKIGNSFANV ALIETVLDFA KTDIPDLEIS HLRVDGDDNV VSINTSCTID 700
RLQRIIKEKY SSLNARVKAL ASYTGLEMAK RFIICGKIFE RGAIPIFTAE 750
RPYGTDVSIQ SMCGSSIYSS AVNAYRGFGD AYFSFMQDVL VPPSASTRIT 800
GRLRVLMSPV TLYATGPLSF EITPQGLGGR CRFFTQSAKL FTLFKMLTQT 850
AAVSITPDEI KKYAETVQFK KRTEVMIASM QRKLLVPAKA LARIIVDKEQ 900
QKTLGVPNVQ SQKNRSQVSK AIEILGVPER NDIVAKGYYP EELYSLIISH 950
SVVVYTSHST PISIYRVNCE PVELLRSQLG IRIADSKPIA KPTNHLYDIV 1000
SGLSPIKISP SDLLTQAKKY DLSTYKGKRD YLSDLGLVGN TLKTYLASKM 1050
LFRDLLMSKY DDLYSTPGFG ATQLTTIPLD VTSAEKVFSI RLGLPPHLYE 1100
VVMLLLLYEY IHYVFSCKRT FTAQMHAISQ EQSAVITKNI ILMLDNIQLD 1150
QVSFSDDAW 1159
Length:1,159
Mass (Da):131,650
Last modified:June 1, 1994 - v1
Checksum:iE355F9BF79E225A8
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
M97203 Genomic RNA. No translation available.
PIRiA44280.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
M97203 Genomic RNA. No translation available.
PIRi A44280.

3D structure databases

ModBasei Search...
MobiDBi Search...

Protocols and materials databases

Structural Biology Knowledgebase Search...

Family and domain databases

InterProi IPR001795. RNA-dir_pol_luteovirus.
IPR007097. RNA-dir_pol_reovirus.
[Graphical view ]
Pfami PF02123. RdRP_4. 1 hit.
[Graphical view ]
PROSITEi PS50523. RDRP_DSRNA_REO. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Sequence analysis of group B rotavirus gene 1 and definition of a rotavirus-specific sequence motif within the RNA polymerase gene."
    Eiden J.J., Hirshon C.
    Virology 192:154-160(1993) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA].

Entry informationi

Entry nameiRDRP_ROTGI
AccessioniPrimary (citable) accession number: P35942
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 1, 1994
Last sequence update: June 1, 1994
Last modified: April 16, 2014
This is version 58 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programViral Protein Annotation Program

Miscellaneousi

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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