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P35901 (RECA_MYCLE) Reviewed, UniProtKB/Swiss-Prot

Last modified May 29, 2013. Version 106. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
Protein RecA
Alternative name(s):
Recombinase A

Cleaved into the following chain:

  1. Mle RecA intein
Gene names
Name:recA
Ordered Locus Names:ML0987
OrganismMycobacterium leprae (strain TN) [Complete proteome] [HAMAP]
Taxonomic identifier272631 [NCBI]
Taxonomic lineageBacteria › Actinobacteria › Actinobacteridae › Actinomycetales › Corynebacterineae › Mycobacteriaceae › Mycobacterium ›

Protein attributes

Sequence length711 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceInferred from homology

General annotation (Comments)

Function

Can catalyze the hydrolysis of ATP in the presence of single-stranded DNA, the ATP-dependent uptake of single-stranded DNA by duplex DNA, and the ATP-dependent hybridization of homologous single-stranded DNAs. It interacts with LexA causing its activation and leading to its autocatalytic cleavage By similarity. HAMAP-Rule MF_00268

Subcellular location

Cytoplasm By similarity HAMAP-Rule MF_00268.

Post-translational modification

This protein undergoes a protein self splicing that involves a post-translational excision of the intervening region (intein) followed by peptide ligation. HAMAP-Rule MF_00268

Sequence similarities

Belongs to the RecA family.

Contains 1 DOD-type homing endonuclease domain.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 205205Protein RecA, 1st part HAMAP-Rule MF_00268
PRO_0000030260
Chain206 – 570365Mle RecA intein HAMAP-Rule MF_00268
PRO_0000030261
Chain571 – 711141Protein RecA, 2nd part HAMAP-Rule MF_00268
PRO_0000030262

Regions

Domain320 – 420101DOD-type homing endonuclease
Nucleotide binding67 – 748ATP By similarity

Sequences

Sequence LengthMass (Da)Tools
P35901 [UniParc].

Last modified June 1, 1994. Version 1.
Checksum: 4D6A5B8B2A115C0E

FASTA71177,638
        10         20         30         40         50         60 
MAQVPDREKA LELAMAQIEK NYGKGSVMRL GDEMCQPISV IPTGSIALDV ALGIGGLPRG 

        70         80         90        100        110        120 
RIVEIYGPES SGKTTVALHA VANAQAVGGV AAFIDAEHAL EPEYAKKLGV DTDSLLVSQP 

       130        140        150        160        170        180 
DTGEQALEIA DMLIRSGALD IVVIDSVAAL VPRAELEGEM GDSYVGLQAR LMSQALRKMT 

       190        200        210        220        230        240 
GALSNSGTTA IFINQLREKI GVMFGCMNYS TRVTLADGST EKIGKIVNNK MDVRVLSYDP 

       250        260        270        280        290        300 
VTDRIVPRKV VNWFNNGPAE QFLQFTVEKS GSNGKSQFAA TPNHLIRTPG GWTEAGNLIA 

       310        320        330        340        350        360 
GDRVLAVEPH MLSDQQFQVV LGSLMGDGNL SPNLCDRNGV RFRLLGYGCK QVEYLQWKKA 

       370        380        390        400        410        420 
LMGNIRHTVR ENSMGASFID FTPLPELVEL QRAVYLGDGK KFLSEEYLKA LTPLVLAIWY 

       430        440        450        460        470        480 
MDDGSFTVGS KRVQERTAGG SGRIEICVDA MTEGTRVRLR DYLCDTHGLD VRLREVGSAG 

       490        500        510        520        530        540 
KAVLVFSTAA TAKFQSLIAP YVAPSMEYKL LPQFRGRGSV TPQFVEPTQQ LVPARVLDVH 

       550        560        570        580        590        600 
VKLSTRSMNR FDIEVEGNHN YFVDGVMVHN SPETTTGGKA LKFYASVRMD VRRIETLKDG 

       610        620        630        640        650        660 
VDAVGNRTRV KIVKNKVSPP FKQAEFDILY GKGISREGSL IDMGVEQGFV RKSGSWFTYE 

       670        680        690        700        710 
GEQLGQGKEN ARNFLLENAD VANEIEKKIK EKLGIGAVVT DDDILPTPVD F

« Hide

References

« Hide 'large scale' references
[1]"Evidence of selection for protein introns in the recAs of pathogenic mycobacteria."
Davis E.O., Thangaraj H.S., Brooks P.C., Colston M.J.
EMBO J. 13:699-703(1994) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]Smith D.R., Robison K.
Submitted (MAR-1994) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[3]"Massive gene decay in the leprosy bacillus."
Cole S.T., Eiglmeier K., Parkhill J., James K.D., Thomson N.R., Wheeler P.R., Honore N., Garnier T., Churcher C.M., Harris D.E., Mungall K.L., Basham D., Brown D., Chillingworth T., Connor R., Davies R.M., Devlin K., Duthoy S. expand/collapse author list , Feltwell T., Fraser A., Hamlin N., Holroyd S., Hornsby T., Jagels K., Lacroix C., Maclean J., Moule S., Murphy L.D., Oliver K., Quail M.A., Rajandream M.A., Rutherford K.M., Rutter S., Seeger K., Simon S., Simmonds M., Skelton J., Squares R., Squares S., Stevens K., Taylor K., Whitehead S., Woodward J.R., Barrell B.G.
Nature 409:1007-1011(2001) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: TN.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		X73822 Genomic DNA. Translation: CAA52040.1.
U00019 Genomic DNA. Translation: AAA17277.1.
AL583920 Genomic DNA. Translation: CAC31368.1.
PIRS37025.
RefSeqNP_301732.1. NC_002677.1.

3D structure databases

ProteinModelPortalP35901.
SMRP35901. Positions 3-211, 538-696.
ModBaseSearch...

Protein-protein interaction databases

STRING272631.ML0987.

Proteomic databases

PRIDEP35901.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaCAC31368; CAC31368; CAC31368.
GeneID910009.
KEGGmle:ML0987.
PATRIC18053688. VBIMycLep78757_1789.

Organism-specific databases

LepromaML0987.
CMRSearch...

Phylogenomic databases

eggNOGCOG1372.
HOGENOMHOG000089956.
KOK03553.

Family and domain databases

Gene3D3.10.28.10. 1 hit.
3.30.250.10. 1 hit.
HAMAPMF_00268. RecA.
InterProIPR003593. AAA+_ATPase.
IPR013765. DNA_recomb/repair_RecA.
IPR020584. DNA_recomb/repair_RecA_CS.
IPR020588. DNA_recomb_RecA/RadB_ATP-bd.
IPR003586. Hint_dom_C.
IPR003587. Hint_dom_N.
IPR027434. Homing_endonucl.
IPR004042. Intein_endonuc.
IPR006141. Intein_splice_site.
IPR004860. LAGLIDADG_2.
IPR027417. P-loop_NTPase.
IPR023400. RecA_C.
IPR020587. RecA_monomer-monomer_interface.
[Graphical view]
PANTHERPTHR22942:SF1. PTHR22942:SF1. 1 hit.
PfamPF03161. LAGLIDADG_2. 1 hit.
PF00154. RecA. 2 hits.
[Graphical view]
PRINTSPR00142. RECA.
SMARTSM00382. AAA. 1 hit.
SM00305. HintC. 1 hit.
SM00306. HintN. 1 hit.
[Graphical view]
SUPFAMSSF52540. SSF52540. 2 hits.
SSF54752. SSF54752. 1 hit.
SSF55608. SSF55608. 1 hit.
TIGRFAMsTIGR01443. intein_Cterm. 1 hit.
TIGR01445. intein_Nterm. 1 hit.
TIGR02012. tigrfam_recA. 1 hit.
PROSITEPS50818. INTEIN_C_TER. 1 hit.
PS50819. INTEIN_ENDONUCLEASE. 1 hit.
PS50817. INTEIN_N_TER. 1 hit.
PS00321. RECA_1. 1 hit.
PS50162. RECA_2. 1 hit.
PS50163. RECA_3. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameRECA_MYCLE
AccessionPrimary (citable) accession number: P35901
Entry history
Integrated into UniProtKB/Swiss-Prot: June 1, 1994
Last sequence update: June 1, 1994
Last modified: May 29, 2013
This is version 106 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

Intein-containing proteins

List of intein-containing protein entries

SIMILARITY comments

Index of protein domains and families

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