Reviewed, UniProtKB/Swiss-Prot P35901 (RECA_MYCLE)

Names and origin

Protein names

Recommended name:
    Protein recA
Alternative name(s):
    Recombinase A
Cleaved into the following chain:
    1- Recommended name:
            Mle recA intein

Gene names

Name:	recA
Ordered Locus Names:	ML0987

Organism

Mycobacterium leprae [Complete proteome] [HAMAP]

Taxonomic identifier

1769 [NCBI]

Taxonomic lineage

Bacteria › Actinobacteria › Actinobacteridae › Actinomycetales › Corynebacterineae › Mycobacteriaceae › Mycobacterium

Protein attributes

Sequence length	711 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Can catalyze the hydrolysis of ATP in the presence of single-stranded DNA, the ATP-dependent uptake of single-stranded DNA by duplex DNA, and the ATP-dependent hybridization of homologous single-stranded DNAs. It interacts with lexA causing its activation and leading to its autocatalytic cleavage. HAMAP MF_00268
Subcellular location	Cytoplasm By similarity.
Post-translational modification	This protein undergoes a protein self splicing that involves a post-translational excision of the intervening region (intein) followed by peptide ligation. HAMAP MF_00268
Sequence similarities	Belongs to the recA family. Contains 1 DOD-type homing endonuclease domain.

Ontologies

Keywords
Biological process	DNA damage DNA recombination DNA repair SOS response
Cellular component	Cytoplasm
Ligand	ATP-binding DNA-binding Nucleotide-binding
PTM	Autocatalytic cleavage Protein splicing
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	DNA recombination Inferred from electronic annotation. Source: HAMAP DNA repair Inferred from electronic annotation. Source: HAMAP SOS response Inferred from electronic annotation. Source: HAMAP intein-mediated protein splicing Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: HAMAP DNA-dependent ATPase activity Inferred from electronic annotation. Source: HAMAP damaged DNA binding Inferred from electronic annotation. Source: HAMAP endonuclease activity Inferred from electronic annotation. Source: InterPro single-stranded DNA binding Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 205	205	Protein recA, 1st part HAMAP MF_00268		PRO_0000030260
	Chain	206 – 570	365	Mle recA intein HAMAP MF_00268		PRO_0000030261
	Chain	571 – 711	141	Protein recA, 2nd part HAMAP MF_00268		PRO_0000030262
Regions
	Domain	320 – 420	101	DOD-type homing endonuclease
	Nucleotide binding	67 – 74	8	ATP By similarity

Sequences

	Sequence		Length	Mass (Da)
	P35901-1 [UniParc]. Last modified June 1, 1994. Version 1. Checksum: 4D6A5B8B2A115C0E	FASTA	711	77,638
	10 20 30 40 50 60 MAQVPDREKA LELAMAQIEK NYGKGSVMRL GDEMCQPISV IPTGSIALDV ALGIGGLPRG 70 80 90 100 110 120 RIVEIYGPES SGKTTVALHA VANAQAVGGV AAFIDAEHAL EPEYAKKLGV DTDSLLVSQP 130 140 150 160 170 180 DTGEQALEIA DMLIRSGALD IVVIDSVAAL VPRAELEGEM GDSYVGLQAR LMSQALRKMT 190 200 210 220 230 240 GALSNSGTTA IFINQLREKI GVMFGCMNYS TRVTLADGST EKIGKIVNNK MDVRVLSYDP 250 260 270 280 290 300 VTDRIVPRKV VNWFNNGPAE QFLQFTVEKS GSNGKSQFAA TPNHLIRTPG GWTEAGNLIA 310 320 330 340 350 360 GDRVLAVEPH MLSDQQFQVV LGSLMGDGNL SPNLCDRNGV RFRLLGYGCK QVEYLQWKKA 370 380 390 400 410 420 LMGNIRHTVR ENSMGASFID FTPLPELVEL QRAVYLGDGK KFLSEEYLKA LTPLVLAIWY 430 440 450 460 470 480 MDDGSFTVGS KRVQERTAGG SGRIEICVDA MTEGTRVRLR DYLCDTHGLD VRLREVGSAG 490 500 510 520 530 540 KAVLVFSTAA TAKFQSLIAP YVAPSMEYKL LPQFRGRGSV TPQFVEPTQQ LVPARVLDVH 550 560 570 580 590 600 VKLSTRSMNR FDIEVEGNHN YFVDGVMVHN SPETTTGGKA LKFYASVRMD VRRIETLKDG 610 620 630 640 650 660 VDAVGNRTRV KIVKNKVSPP FKQAEFDILY GKGISREGSL IDMGVEQGFV RKSGSWFTYE 670 680 690 700 710 GEQLGQGKEN ARNFLLENAD VANEIEKKIK EKLGIGAVVT DDDILPTPVD F

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Evidence of selection for protein introns in the recAs of pathogenic mycobacteria." Davis E.O., Thangaraj H.S., Brooks P.C., Colston M.J. EMBO J. 13:699-703(1994) [PubMed: 7508863] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	Smith D.R., Robison K. Submitted (MAR-1994) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[3]	"Massive gene decay in the leprosy bacillus." Cole S.T., Eiglmeier K., Parkhill J., James K.D., Thomson N.R., Wheeler P.R., Honore N., Garnier T., Churcher C.M., Harris D.E., Mungall K.L., Basham D., Brown D., Chillingworth T., Connor R., Davies R.M., Devlin K., Duthoy S. , Feltwell T., Fraser A., Hamlin N., Holroyd S., Hornsby T., Jagels K., Lacroix C., Maclean J., Moule S., Murphy L.D., Oliver K., Quail M.A., Rajandream M.A., Rutherford K.M., Rutter S., Seeger K., Simon S., Simmonds M., Skelton J., Squares R., Squares S., Stevens K., Taylor K., Whitehead S., Woodward J.R., Barrell B.G. Nature 409:1007-1011(2001) [PubMed: 11234002] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: TN.

Cross-references

Sequence databases
EMBLGenBankDDBJ	X73822 Genomic DNA. Translation: CAA52040.1. U00019 Genomic DNA. Translation: AAA17277.1. AL583920 Genomic DNA. Translation: CAC31368.1.
PIR	S37025.
RefSeq	NP_301732.1.
3D structure databases
SMR	P35901. Positions 1-211, 518-694.
ModBase	Search...
Genome annotation databases
GeneID	910009.
GenomeReviews	Gene locus ML0987 in contig AL450380_GR.
KEGG	mle:ML0987.
NMPDR	fig|272631.1.peg.604.
Organism-specific databases
Leproma	ML0987.
CMR	Search...
Enzyme and pathway databases
BioCyc	MLEP272631:ML0987-MON.
Family and domain databases
HAMAP	MF_00268. [Tree]
InterPro	IPR003593. ATPase_AAA+_core. IPR013765. DNA_recomb/repair_RecA. IPR001553. DNA_recomb/repair_RecA/RadB. IPR020584. DNA_recomb/repair_RecA_CS. IPR020588. DNA_recomb_RecA/RadB_ATP-bd. IPR003586. Hedgehog_hint_C. IPR003587. Hedgehog_hint_N. IPR004042. Intein_endonuc. IPR006141. Intein_splicing_site. IPR004860. LAGLIDADG_2. IPR020587. RecA_monomer-monomer_interface. [Graphical view]
PANTHER	PTHR22942:SF1. RecA. 1 hit.
Pfam	PF03161. LAGLIDADG_2. 1 hit. PF00154. RecA. 2 hits. [Graphical view]
PRINTS	PR00142. RECA.
SMART	SM00382. AAA. 1 hit. SM00305. HintC. 1 hit. SM00306. HintN. 1 hit. [Graphical view]
TIGRFAMs	TIGR01443. intein_Cterm. 1 hit. TIGR01445. intein_Nterm. 1 hit. TIGR02012. tigrfam_recA. 1 hit.
PROSITE	PS50818. INTEIN_C_TER. 1 hit. PS50819. INTEIN_ENDONUCLEASE. 1 hit. PS50817. INTEIN_N_TER. 1 hit. PS00321. RECA_1. 1 hit. PS50162. RECA_2. 1 hit. PS50163. RECA_3. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

RECA_MYCLE

Accession

Primary (citable) accession number: P35901

Entry history

Integrated into UniProtKB/Swiss-Prot:	June 1, 1994
Last sequence update:	June 1, 1994
Last modified:	November 24, 2009
This is version 79 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)