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Protein

Protein RecA

Gene

recA

Organism
Mycobacterium leprae (strain TN)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Can catalyze the hydrolysis of ATP in the presence of single-stranded DNA, the ATP-dependent uptake of single-stranded DNA by duplex DNA, and the ATP-dependent hybridization of homologous single-stranded DNAs. It interacts with LexA causing its activation and leading to its autocatalytic cleavage (By similarity).By similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi67 – 748ATPBy similarity

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Biological processi

DNA damage, DNA recombination, DNA repair, SOS response

Keywords - Ligandi

ATP-binding, DNA-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Protein RecA
Alternative name(s):
Recombinase A
Cleaved into the following chain:
Gene namesi
Name:recA
Ordered Locus Names:ML0987
OrganismiMycobacterium leprae (strain TN)
Taxonomic identifieri272631 [NCBI]
Taxonomic lineageiBacteriaActinobacteriaCorynebacterialesMycobacteriaceaeMycobacterium
ProteomesiUP000000806 Componenti: Chromosome

Organism-specific databases

LepromaiML0987.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 205205Protein RecA, 1st partPRO_0000030260Add
BLAST
Chaini206 – 570365Mle RecA inteinPRO_0000030261Add
BLAST
Chaini571 – 711141Protein RecA, 2nd partPRO_0000030262Add
BLAST

Post-translational modificationi

This protein undergoes a protein self splicing that involves a post-translational excision of the intervening region (intein) followed by peptide ligation.

Keywords - PTMi

Autocatalytic cleavage, Protein splicing

Proteomic databases

PRIDEiP35901.

Interactioni

Protein-protein interaction databases

STRINGi272631.ML0987.

Structurei

3D structure databases

ProteinModelPortaliP35901.
SMRiP35901. Positions 3-211, 538-696.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini320 – 420101DOD-type homing endonucleaseAdd
BLAST

Sequence similaritiesi

Belongs to the RecA family.Curated

Phylogenomic databases

eggNOGiCOG1372.
HOGENOMiHOG000089956.
KOiK03553.
OMAiDQGTQDV.
OrthoDBiEOG6ZKXNZ.

Family and domain databases

Gene3Di2.170.16.10. 2 hits.
3.10.28.10. 1 hit.
3.30.250.10. 1 hit.
3.40.50.300. 2 hits.
HAMAPiMF_00268. RecA.
InterProiIPR003593. AAA+_ATPase.
IPR013765. DNA_recomb/repair_RecA.
IPR020584. DNA_recomb/repair_RecA_CS.
IPR028992. Hedgehog/Intein_dom.
IPR003586. Hint_dom_C.
IPR003587. Hint_dom_N.
IPR027434. Homing_endonucl.
IPR030934. Intein_C.
IPR004042. Intein_endonuc.
IPR006141. Intein_N.
IPR004860. LAGLIDADG_2.
IPR027417. P-loop_NTPase.
IPR020588. RecA_ATP-bd.
IPR023400. RecA_C.
IPR020587. RecA_monomer-monomer_interface.
[Graphical view]
PANTHERiPTHR22942:SF1. PTHR22942:SF1. 1 hit.
PfamiPF03161. LAGLIDADG_2. 1 hit.
PF00154. RecA. 2 hits.
[Graphical view]
PRINTSiPR00142. RECA.
SMARTiSM00382. AAA. 1 hit.
SM00305. HintC. 1 hit.
SM00306. HintN. 1 hit.
[Graphical view]
SUPFAMiSSF51294. SSF51294. 2 hits.
SSF52540. SSF52540. 2 hits.
SSF54752. SSF54752. 1 hit.
SSF55608. SSF55608. 1 hit.
TIGRFAMsiTIGR01443. intein_Cterm. 1 hit.
TIGR01445. intein_Nterm. 1 hit.
TIGR02012. tigrfam_recA. 1 hit.
PROSITEiPS50818. INTEIN_C_TER. 1 hit.
PS50819. INTEIN_ENDONUCLEASE. 1 hit.
PS50817. INTEIN_N_TER. 1 hit.
PS00321. RECA_1. 1 hit.
PS50162. RECA_2. 1 hit.
PS50163. RECA_3. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P35901-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAQVPDREKA LELAMAQIEK NYGKGSVMRL GDEMCQPISV IPTGSIALDV
60 70 80 90 100
ALGIGGLPRG RIVEIYGPES SGKTTVALHA VANAQAVGGV AAFIDAEHAL
110 120 130 140 150
EPEYAKKLGV DTDSLLVSQP DTGEQALEIA DMLIRSGALD IVVIDSVAAL
160 170 180 190 200
VPRAELEGEM GDSYVGLQAR LMSQALRKMT GALSNSGTTA IFINQLREKI
210 220 230 240 250
GVMFGCMNYS TRVTLADGST EKIGKIVNNK MDVRVLSYDP VTDRIVPRKV
260 270 280 290 300
VNWFNNGPAE QFLQFTVEKS GSNGKSQFAA TPNHLIRTPG GWTEAGNLIA
310 320 330 340 350
GDRVLAVEPH MLSDQQFQVV LGSLMGDGNL SPNLCDRNGV RFRLLGYGCK
360 370 380 390 400
QVEYLQWKKA LMGNIRHTVR ENSMGASFID FTPLPELVEL QRAVYLGDGK
410 420 430 440 450
KFLSEEYLKA LTPLVLAIWY MDDGSFTVGS KRVQERTAGG SGRIEICVDA
460 470 480 490 500
MTEGTRVRLR DYLCDTHGLD VRLREVGSAG KAVLVFSTAA TAKFQSLIAP
510 520 530 540 550
YVAPSMEYKL LPQFRGRGSV TPQFVEPTQQ LVPARVLDVH VKLSTRSMNR
560 570 580 590 600
FDIEVEGNHN YFVDGVMVHN SPETTTGGKA LKFYASVRMD VRRIETLKDG
610 620 630 640 650
VDAVGNRTRV KIVKNKVSPP FKQAEFDILY GKGISREGSL IDMGVEQGFV
660 670 680 690 700
RKSGSWFTYE GEQLGQGKEN ARNFLLENAD VANEIEKKIK EKLGIGAVVT
710
DDDILPTPVD F
Length:711
Mass (Da):77,638
Last modified:June 1, 1994 - v1
Checksum:i4D6A5B8B2A115C0E
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X73822 Genomic DNA. Translation: CAA52040.1.
U00019 Genomic DNA. Translation: AAA17277.1.
AL583920 Genomic DNA. Translation: CAC31368.1.
PIRiS37025.
RefSeqiNP_301732.1. NC_002677.1.
WP_010908056.1. NC_002677.1.

Genome annotation databases

EnsemblBacteriaiCAC31368; CAC31368; CAC31368.
GeneIDi910009.
KEGGimle:ML0987.
PATRICi18053688. VBIMycLep78757_1789.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X73822 Genomic DNA. Translation: CAA52040.1.
U00019 Genomic DNA. Translation: AAA17277.1.
AL583920 Genomic DNA. Translation: CAC31368.1.
PIRiS37025.
RefSeqiNP_301732.1. NC_002677.1.
WP_010908056.1. NC_002677.1.

3D structure databases

ProteinModelPortaliP35901.
SMRiP35901. Positions 3-211, 538-696.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi272631.ML0987.

Proteomic databases

PRIDEiP35901.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiCAC31368; CAC31368; CAC31368.
GeneIDi910009.
KEGGimle:ML0987.
PATRICi18053688. VBIMycLep78757_1789.

Organism-specific databases

LepromaiML0987.

Phylogenomic databases

eggNOGiCOG1372.
HOGENOMiHOG000089956.
KOiK03553.
OMAiDQGTQDV.
OrthoDBiEOG6ZKXNZ.

Family and domain databases

Gene3Di2.170.16.10. 2 hits.
3.10.28.10. 1 hit.
3.30.250.10. 1 hit.
3.40.50.300. 2 hits.
HAMAPiMF_00268. RecA.
InterProiIPR003593. AAA+_ATPase.
IPR013765. DNA_recomb/repair_RecA.
IPR020584. DNA_recomb/repair_RecA_CS.
IPR028992. Hedgehog/Intein_dom.
IPR003586. Hint_dom_C.
IPR003587. Hint_dom_N.
IPR027434. Homing_endonucl.
IPR030934. Intein_C.
IPR004042. Intein_endonuc.
IPR006141. Intein_N.
IPR004860. LAGLIDADG_2.
IPR027417. P-loop_NTPase.
IPR020588. RecA_ATP-bd.
IPR023400. RecA_C.
IPR020587. RecA_monomer-monomer_interface.
[Graphical view]
PANTHERiPTHR22942:SF1. PTHR22942:SF1. 1 hit.
PfamiPF03161. LAGLIDADG_2. 1 hit.
PF00154. RecA. 2 hits.
[Graphical view]
PRINTSiPR00142. RECA.
SMARTiSM00382. AAA. 1 hit.
SM00305. HintC. 1 hit.
SM00306. HintN. 1 hit.
[Graphical view]
SUPFAMiSSF51294. SSF51294. 2 hits.
SSF52540. SSF52540. 2 hits.
SSF54752. SSF54752. 1 hit.
SSF55608. SSF55608. 1 hit.
TIGRFAMsiTIGR01443. intein_Cterm. 1 hit.
TIGR01445. intein_Nterm. 1 hit.
TIGR02012. tigrfam_recA. 1 hit.
PROSITEiPS50818. INTEIN_C_TER. 1 hit.
PS50819. INTEIN_ENDONUCLEASE. 1 hit.
PS50817. INTEIN_N_TER. 1 hit.
PS00321. RECA_1. 1 hit.
PS50162. RECA_2. 1 hit.
PS50163. RECA_3. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Evidence of selection for protein introns in the recAs of pathogenic mycobacteria."
    Davis E.O., Thangaraj H.S., Brooks P.C., Colston M.J.
    EMBO J. 13:699-703(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  2. Smith D.R., Robison K.
    Submitted (MAR-1994) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: TN.

Entry informationi

Entry nameiRECA_MYCLE
AccessioniPrimary (citable) accession number: P35901
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 1, 1994
Last sequence update: June 1, 1994
Last modified: June 24, 2015
This is version 120 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Intein-containing proteins
    List of intein-containing protein entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.