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P35900

- K1C20_HUMAN

UniProt

P35900 - K1C20_HUMAN

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Protein
Keratin, type I cytoskeletal 20
Gene
KRT20
Organism
Homo sapiens (Human)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at protein leveli

Functioni

Plays a significant role in maintaining keratin filament organization in intestinal epithelia. When phosphorylated, plays a role in the secretion of mucin in the small intestine By similarity.2 Publications

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sitei228 – 2292Cleavage; by caspases

GO - Molecular functioni

  1. protein binding Source: UniProtKB
  2. structural constituent of cytoskeleton Source: UniProtKB

GO - Biological processi

  1. apoptotic process Source: UniProtKB-KW
  2. cellular response to stress Source: Ensembl
  3. intermediate filament organization Source: UniProtKB
  4. regulation of protein secretion Source: Ensembl
Complete GO annotation...

Keywords - Biological processi

Apoptosis

Names & Taxonomyi

Protein namesi
Recommended name:
Keratin, type I cytoskeletal 20
Alternative name(s):
Cytokeratin-20
Short name:
CK-20
Keratin-20
Short name:
K20
Protein IT
Gene namesi
Name:KRT20
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
ProteomesiUP000005640: Chromosome 17

Organism-specific databases

HGNCiHGNC:20412. KRT20.

Subcellular locationi

Cytoplasm 2 Publications

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB
  2. intermediate filament Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Intermediate filament, Keratin

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi13 – 131S → A: Promotes keratin filament disassembly. 1 Publication
Mutagenesisi14 – 141S → A: No effect on keratin filament organization. 1 Publication
Mutagenesisi80 – 801R → H: Leads to collapsed filaments. 1 Publication

Organism-specific databases

PharmGKBiPA134938907.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 424424Keratin, type I cytoskeletal 20
PRO_0000063675Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei13 – 131Phosphoserine; by MAPKAPK2, MAPKAPK3 and PKC1 Publication

Post-translational modificationi

Hyperphosphorylation at Ser-13 occurs during the early stages of apoptosis but becomes less prominent during the later stages. Phosphorylation at Ser-13 also increases in response to stress brought on by cell injury By similarity.
Proteolytically cleaved by caspases during apoptosis. Cleavage occurs at Asp-228.1 Publication

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiP35900.
PaxDbiP35900.
PRIDEiP35900.

PTM databases

PhosphoSiteiP35900.

Expressioni

Tissue specificityi

Expressed predominantly in the intestinal epithelium. Expressed in luminal cells of colonic mucosa. Also expressed in the Merkel cells of keratinized oral mucosa; specifically at the tips of some rete ridges of the gingival mucosa, in the basal layer of the palatal mucosa and in the taste buds of lingual mucosa.2 Publications

Developmental stagei

First detected at embryonic week 8 in individual 'converted' simple epithelial cells of the developing intestinal mucosa. In later fetal stages, synthesis extends over most goblet cells and a variable number of villus enterocytes. In the developing gastric and intestinal mucosa, expressed in all enterocytes and goblet cells as well as certain 'low-differentiated' columnar cells, whereas the neuroendocrine and Paneth cells are negative.1 Publication

Gene expression databases

BgeeiP35900.
CleanExiHS_KRT20.
GenevestigatoriP35900.

Organism-specific databases

HPAiCAB000032.
HPA024309.
HPA024684.
HPA027236.

Interactioni

Subunit structurei

Heterotetramer of two type I and two type II keratins. Associates with KRT8.

Protein-protein interaction databases

BioGridi119979. 17 interactions.
IntActiP35900. 16 interactions.
MINTiMINT-1445560.
STRINGi9606.ENSP00000167588.

Structurei

3D structure databases

ProteinModelPortaliP35900.
SMRiP35900. Positions 287-376.

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni1 – 6969Head
Add
BLAST
Regioni70 – 377308Rod
Add
BLAST
Regioni70 – 10536Coil 1A
Add
BLAST
Regioni106 – 12318Linker 1
Add
BLAST
Regioni124 – 21592Coil 1B
Add
BLAST
Regioni216 – 23823Linker 12
Add
BLAST
Regioni239 – 377139Coil 2
Add
BLAST
Regioni378 – 42447Tail
Add
BLAST

Sequence similaritiesi

Keywords - Domaini

Coiled coil

Phylogenomic databases

eggNOGiNOG149615.
HOGENOMiHOG000230975.
HOVERGENiHBG013015.
InParanoidiP35900.
KOiK07604.
OMAiQIKQWYE.
OrthoDBiEOG7FV3Q8.
PhylomeDBiP35900.
TreeFamiTF332742.

Family and domain databases

InterProiIPR001664. IF.
IPR018039. Intermediate_filament_CS.
IPR002957. Keratin_I.
[Graphical view]
PANTHERiPTHR23239. PTHR23239. 1 hit.
PfamiPF00038. Filament. 1 hit.
[Graphical view]
PRINTSiPR01248. TYPE1KERATIN.
PROSITEiPS00226. IF. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P35900-1 [UniParc]FASTAAdd to Basket

« Hide

MDFSRRSFHR SLSSSLQAPV VSTVGMQRLG TTPSVYGGAG GRGIRISNSR    50
HTVNYGSDLT GGGDLFVGNE KMAMQNLNDR LASYLEKVRT LEQSNSKLEV 100
QIKQWYETNA PRAGRDYSAY YRQIEELRSQ IKDAQLQNAR CVLQIDNAKL 150
AAEDFRLKYE TERGIRLTVE ADLQGLNKVF DDLTLHKTDL EIQIEELNKD 200
LALLKKEHQE EVDGLHKHLG NTVNVEVDAA PGLNLGVIMN EMRQKYEVMA 250
QKNLQEAKEQ FERQTAVLQQ QVTVNTEELK GTEVQLTELR RTSQSLEIEL 300
QSHLSMKESL EHTLEETKAR YSSQLANLQS LLSSLEAQLM QIRSNMERQN 350
NEYHILLDIK TRLEQEIATY RRLLEGEDVK TTEYQLSTLE ERDIKKTRKI 400
KTVVQEVVDG KVVSSEVKEV EENI 424
Length:424
Mass (Da):48,487
Last modified:June 1, 1994 - v1
Checksum:iA8EF5A518C73CCE5
GO

Natural variant

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Natural varianti4 – 41S → R in a colorectal cancer sample; somatic mutation. 1 Publication
VAR_036367
Natural varianti129 – 1291S → N.1 Publication
Corresponds to variant rs7212483 [ dbSNP | Ensembl ].
VAR_024489
Natural varianti214 – 2141G → C Found in a renal cell carcinoma sample; somatic mutation. 1 Publication
VAR_064726

Sequence conflict

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti161 – 1611T → S in CAA51914. 1 Publication
Sequence conflicti349 – 3491Q → P in CAA51914. 1 Publication
Sequence conflicti398 – 3981R → T in CAA51914. 1 Publication

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
X73501 Genomic DNA. Translation: CAA51913.1.
AK312744 mRNA. Translation: BAG35614.1.
BC031559 mRNA. Translation: AAH31559.1.
X73502 mRNA. Translation: CAA51914.1.
CCDSiCCDS11379.1.
PIRiS37780.
RefSeqiNP_061883.1. NM_019010.2.
UniGeneiHs.84905.

Genome annotation databases

EnsembliENST00000167588; ENSP00000167588; ENSG00000171431.
ENST00000576098; ENSP00000460501; ENSG00000263057.
GeneIDi54474.
KEGGihsa:54474.
UCSCiuc002hvl.3. human.

Polymorphism databases

DMDMi547750.

Keywords - Coding sequence diversityi

Polymorphism

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
X73501 Genomic DNA. Translation: CAA51913.1 .
AK312744 mRNA. Translation: BAG35614.1 .
BC031559 mRNA. Translation: AAH31559.1 .
X73502 mRNA. Translation: CAA51914.1 .
CCDSi CCDS11379.1.
PIRi S37780.
RefSeqi NP_061883.1. NM_019010.2.
UniGenei Hs.84905.

3D structure databases

ProteinModelPortali P35900.
SMRi P35900. Positions 287-376.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 119979. 17 interactions.
IntActi P35900. 16 interactions.
MINTi MINT-1445560.
STRINGi 9606.ENSP00000167588.

PTM databases

PhosphoSitei P35900.

Polymorphism databases

DMDMi 547750.

Proteomic databases

MaxQBi P35900.
PaxDbi P35900.
PRIDEi P35900.

Protocols and materials databases

DNASUi 54474.
Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENST00000167588 ; ENSP00000167588 ; ENSG00000171431 .
ENST00000576098 ; ENSP00000460501 ; ENSG00000263057 .
GeneIDi 54474.
KEGGi hsa:54474.
UCSCi uc002hvl.3. human.

Organism-specific databases

CTDi 54474.
GeneCardsi GC17M039030.
HGNCi HGNC:20412. KRT20.
HPAi CAB000032.
HPA024309.
HPA024684.
HPA027236.
MIMi 608218. gene.
neXtProti NX_P35900.
PharmGKBi PA134938907.
GenAtlasi Search...

Phylogenomic databases

eggNOGi NOG149615.
HOGENOMi HOG000230975.
HOVERGENi HBG013015.
InParanoidi P35900.
KOi K07604.
OMAi QIKQWYE.
OrthoDBi EOG7FV3Q8.
PhylomeDBi P35900.
TreeFami TF332742.

Miscellaneous databases

GeneWikii Keratin_20.
GenomeRNAii 54474.
NextBioi 56776.
PROi P35900.
SOURCEi Search...

Gene expression databases

Bgeei P35900.
CleanExi HS_KRT20.
Genevestigatori P35900.

Family and domain databases

InterProi IPR001664. IF.
IPR018039. Intermediate_filament_CS.
IPR002957. Keratin_I.
[Graphical view ]
PANTHERi PTHR23239. PTHR23239. 1 hit.
Pfami PF00038. Filament. 1 hit.
[Graphical view ]
PRINTSi PR01248. TYPE1KERATIN.
PROSITEi PS00226. IF. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "The human gene encoding cytokeratin 20 and its expression during fetal development and in gastrointestinal carcinomas."
    Moll R., Zimbelmann R., Goldschmidt M.D., Keith M., Laufer J., Kasper M., Koch P.J., Franke W.W.
    Differentiation 53:75-93(1993) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], DEVELOPMENTAL STAGE.
    Tissue: Intestine.
  2. "Complete sequencing and characterization of 21,243 full-length human cDNAs."
    Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S.
    , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S.
    Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA], VARIANT ASN-129.
    Tissue: Small intestine.
  3. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
  4. "Keratin 20 serine 13 phosphorylation is a stress and intestinal goblet cell marker."
    Zhou Q., Cadrin M., Herrmann H., Chen C.-H., Chalkley R.J., Burlingame A.L., Omary M.B.
    J. Biol. Chem. 281:16453-16461(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: PROTEIN SEQUENCE OF 11-28, FUNCTION, INTERACTION WITH KRT8, PHOSPHORYLATION AT SER-13, CLEAVAGE AT ASP-228, MUTAGENESIS OF SER-13 AND SER-14.
  5. "Differential localization by in situ hybridization of distinct keratin mRNA species during intestinal epithelial cell development and differentiation."
    Calnek D., Quaroni A.
    Differentiation 53:95-104(1993) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 86-424.
    Tissue: Intestine.
  6. "Identification of protein IT of the intestinal cytoskeleton as a novel type I cytokeratin with unusual properties and expression patterns."
    Moll R., Schiller D.L., Franke W.W.
    J. Cell Biol. 111:567-580(1990) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION, SUBCELLULAR LOCATION, TISSUE SPECIFICITY.
  7. "An immunohistological study of cytokeratin 20 in human and mammalian oral epithelium."
    Barrett A.W., Cort E.M., Patel P., Berkovitz B.K.B.
    Arch. Oral Biol. 45:879-887(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH KRT8, SUBCELLULAR LOCATION, TISSUE SPECIFICITY.
  8. "Keratin 20 helps maintain intermediate filament organization in intestinal epithelia."
    Zhou Q., Toivola D.M., Feng N., Greenberg H.B., Franke W.W., Omary M.B.
    Mol. Biol. Cell 14:2959-2971(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, MUTAGENESIS OF ARG-80.
  9. Cited for: VARIANT [LARGE SCALE ANALYSIS] ARG-4.
  10. Cited for: VARIANT CYS-214.

Entry informationi

Entry nameiK1C20_HUMAN
AccessioniPrimary (citable) accession number: P35900
Secondary accession number(s): B2R6W7
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 1, 1994
Last sequence update: June 1, 1994
Last modified: July 9, 2014
This is version 135 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Miscellaneous

There are two types of cytoskeletal and microfibrillar keratin: I (acidic; 40-55 kDa) and II (neutral to basic; 56-70 kDa).

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Human chromosome 17
    Human chromosome 17: entries, gene names and cross-references to MIM
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  5. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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