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P35900 (K1C20_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Last modified March 19, 2014. Version 132. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (5) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Keratin, type I cytoskeletal 20
Alternative name(s):
Cytokeratin-20
Short name=CK-20
Keratin-20
Short name=K20
Protein IT
Gene names
Name:KRT20
OrganismHomo sapiens (Human) [Reference proteome]
Taxonomic identifier9606 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo

Protein attributes

Sequence length424 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Plays a significant role in maintaining keratin filament organization in intestinal epithelia. When phosphorylated, plays a role in the secretion of mucin in the small intestine By similarity. Ref.4 Ref.8

Subunit structure

Heterotetramer of two type I and two type II keratins. Associates with KRT8.

Subcellular location

Cytoplasm Ref.6 Ref.7.

Tissue specificity

Expressed predominantly in the intestinal epithelium. Expressed in luminal cells of colonic mucosa. Also expressed in the Merkel cells of keratinized oral mucosa; specifically at the tips of some rete ridges of the gingival mucosa, in the basal layer of the palatal mucosa and in the taste buds of lingual mucosa. Ref.6 Ref.7

Developmental stage

First detected at embryonic week 8 in individual 'converted' simple epithelial cells of the developing intestinal mucosa. In later fetal stages, synthesis extends over most goblet cells and a variable number of villus enterocytes. In the developing gastric and intestinal mucosa, expressed in all enterocytes and goblet cells as well as certain 'low-differentiated' columnar cells, whereas the neuroendocrine and Paneth cells are negative. Ref.1

Post-translational modification

Hyperphosphorylation at Ser-13 occurs during the early stages of apoptosis but becomes less prominent during the later stages. Phosphorylation at Ser-13 also increases in response to stress brought on by cell injury By similarity.

Proteolytically cleaved by caspases during apoptosis. Cleavage occurs at Asp-228. Ref.4

Miscellaneous

There are two types of cytoskeletal and microfibrillar keratin: I (acidic; 40-55 kDa) and II (neutral to basic; 56-70 kDa).

Sequence similarities

Belongs to the intermediate filament family.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 424424Keratin, type I cytoskeletal 20
PRO_0000063675

Regions

Region1 – 6969Head
Region70 – 377308Rod
Region70 – 10536Coil 1A
Region106 – 12318Linker 1
Region124 – 21592Coil 1B
Region216 – 23823Linker 12
Region239 – 377139Coil 2
Region378 – 42447Tail

Sites

Site228 – 2292Cleavage; by caspases

Amino acid modifications

Modified residue131Phosphoserine; by MAPKAPK2, MAPKAPK3 and PKC Ref.4

Natural variations

Natural variant41S → R in a colorectal cancer sample; somatic mutation. Ref.9
VAR_036367
Natural variant1291S → N. Ref.2
Corresponds to variant rs7212483 [ dbSNP | Ensembl ].
VAR_024489
Natural variant2141G → C Found in a renal cell carcinoma sample; somatic mutation. Ref.10
VAR_064726

Experimental info

Mutagenesis131S → A: Promotes keratin filament disassembly. Ref.4
Mutagenesis141S → A: No effect on keratin filament organization. Ref.4
Mutagenesis801R → H: Leads to collapsed filaments. Ref.8
Sequence conflict1611T → S in CAA51914. Ref.5
Sequence conflict3491Q → P in CAA51914. Ref.5
Sequence conflict3981R → T in CAA51914. Ref.5

Sequences

Sequence LengthMass (Da)Tools
P35900 [UniParc].

Last modified June 1, 1994. Version 1.
Checksum: A8EF5A518C73CCE5

FASTA42448,487
        10         20         30         40         50         60 
MDFSRRSFHR SLSSSLQAPV VSTVGMQRLG TTPSVYGGAG GRGIRISNSR HTVNYGSDLT 

        70         80         90        100        110        120 
GGGDLFVGNE KMAMQNLNDR LASYLEKVRT LEQSNSKLEV QIKQWYETNA PRAGRDYSAY 

       130        140        150        160        170        180 
YRQIEELRSQ IKDAQLQNAR CVLQIDNAKL AAEDFRLKYE TERGIRLTVE ADLQGLNKVF 

       190        200        210        220        230        240 
DDLTLHKTDL EIQIEELNKD LALLKKEHQE EVDGLHKHLG NTVNVEVDAA PGLNLGVIMN 

       250        260        270        280        290        300 
EMRQKYEVMA QKNLQEAKEQ FERQTAVLQQ QVTVNTEELK GTEVQLTELR RTSQSLEIEL 

       310        320        330        340        350        360 
QSHLSMKESL EHTLEETKAR YSSQLANLQS LLSSLEAQLM QIRSNMERQN NEYHILLDIK 

       370        380        390        400        410        420 
TRLEQEIATY RRLLEGEDVK TTEYQLSTLE ERDIKKTRKI KTVVQEVVDG KVVSSEVKEV 


EENI 

« Hide

References

« Hide 'large scale' references
[1]"The human gene encoding cytokeratin 20 and its expression during fetal development and in gastrointestinal carcinomas."
Moll R., Zimbelmann R., Goldschmidt M.D., Keith M., Laufer J., Kasper M., Koch P.J., Franke W.W.
Differentiation 53:75-93(1993) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], DEVELOPMENTAL STAGE.
Tissue: Intestine.
[2]"Complete sequencing and characterization of 21,243 full-length human cDNAs."
Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. expand/collapse author list , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S.
Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA], VARIANT ASN-129.
Tissue: Small intestine.
[3]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
[4]"Keratin 20 serine 13 phosphorylation is a stress and intestinal goblet cell marker."
Zhou Q., Cadrin M., Herrmann H., Chen C.-H., Chalkley R.J., Burlingame A.L., Omary M.B.
J. Biol. Chem. 281:16453-16461(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: PROTEIN SEQUENCE OF 11-28, FUNCTION, INTERACTION WITH KRT8, PHOSPHORYLATION AT SER-13, CLEAVAGE AT ASP-228, MUTAGENESIS OF SER-13 AND SER-14.
[5]"Differential localization by in situ hybridization of distinct keratin mRNA species during intestinal epithelial cell development and differentiation."
Calnek D., Quaroni A.
Differentiation 53:95-104(1993) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 86-424.
Tissue: Intestine.
[6]"Identification of protein IT of the intestinal cytoskeleton as a novel type I cytokeratin with unusual properties and expression patterns."
Moll R., Schiller D.L., Franke W.W.
J. Cell Biol. 111:567-580(1990) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION, SUBCELLULAR LOCATION, TISSUE SPECIFICITY.
[7]"An immunohistological study of cytokeratin 20 in human and mammalian oral epithelium."
Barrett A.W., Cort E.M., Patel P., Berkovitz B.K.B.
Arch. Oral Biol. 45:879-887(2000) [PubMed] [Europe PMC] [Abstract]
Cited for: INTERACTION WITH KRT8, SUBCELLULAR LOCATION, TISSUE SPECIFICITY.
[8]"Keratin 20 helps maintain intermediate filament organization in intestinal epithelia."
Zhou Q., Toivola D.M., Feng N., Greenberg H.B., Franke W.W., Omary M.B.
Mol. Biol. Cell 14:2959-2971(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, MUTAGENESIS OF ARG-80.
[9]"The consensus coding sequences of human breast and colorectal cancers."
Sjoeblom T., Jones S., Wood L.D., Parsons D.W., Lin J., Barber T.D., Mandelker D., Leary R.J., Ptak J., Silliman N., Szabo S., Buckhaults P., Farrell C., Meeh P., Markowitz S.D., Willis J., Dawson D., Willson J.K.V. expand/collapse author list , Gazdar A.F., Hartigan J., Wu L., Liu C., Parmigiani G., Park B.H., Bachman K.E., Papadopoulos N., Vogelstein B., Kinzler K.W., Velculescu V.E.
Science 314:268-274(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: VARIANT [LARGE SCALE ANALYSIS] ARG-4.
[10]"Exome sequencing identifies frequent mutation of the SWI/SNF complex gene PBRM1 in renal carcinoma."
Varela I., Tarpey P., Raine K., Huang D., Ong C.K., Stephens P., Davies H., Jones D., Lin M.L., Teague J., Bignell G., Butler A., Cho J., Dalgliesh G.L., Galappaththige D., Greenman C., Hardy C., Jia M. expand/collapse author list , Latimer C., Lau K.W., Marshall J., McLaren S., Menzies A., Mudie L., Stebbings L., Largaespada D.A., Wessels L.F.A., Richard S., Kahnoski R.J., Anema J., Tuveson D.A., Perez-Mancera P.A., Mustonen V., Fischer A., Adams D.J., Rust A., Chan-On W., Subimerb C., Dykema K., Furge K., Campbell P.J., Teh B.T., Stratton M.R., Futreal P.A.
Nature 469:539-542(2011) [PubMed] [Europe PMC] [Abstract]
Cited for: VARIANT CYS-214.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
X73501 Genomic DNA. Translation: CAA51913.1.
AK312744 mRNA. Translation: BAG35614.1.
BC031559 mRNA. Translation: AAH31559.1.
X73502 mRNA. Translation: CAA51914.1.
PIRS37780.
RefSeqNP_061883.1. NM_019010.2.
UniGeneHs.84905.

3D structure databases

ProteinModelPortalP35900.
SMRP35900. Positions 68-208, 235-376.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid119979. 17 interactions.
IntActP35900. 16 interactions.
MINTMINT-1445560.
STRING9606.ENSP00000167588.

PTM databases

PhosphoSiteP35900.

Polymorphism databases

DMDM547750.

Proteomic databases

PaxDbP35900.
PRIDEP35900.

Protocols and materials databases

DNASU54474.
StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENST00000167588; ENSP00000167588; ENSG00000171431.
ENST00000576098; ENSP00000460501; ENSG00000263057.
GeneID54474.
KEGGhsa:54474.
UCSCuc002hvl.3. human.

Organism-specific databases

CTD54474.
GeneCardsGC17M039030.
HGNCHGNC:20412. KRT20.
HPACAB000032.
HPA024309.
HPA024684.
HPA027236.
MIM608218. gene.
neXtProtNX_P35900.
PharmGKBPA134938907.
GenAtlasSearch...

Phylogenomic databases

eggNOGNOG149615.
HOGENOMHOG000230975.
HOVERGENHBG013015.
InParanoidP35900.
KOK07604.
OMAQIKQWYE.
OrthoDBEOG7FV3Q8.
PhylomeDBP35900.
TreeFamTF332742.

Gene expression databases

BgeeP35900.
CleanExHS_KRT20.
GenevestigatorP35900.

Family and domain databases

InterProIPR001664. IF.
IPR018039. Intermediate_filament_CS.
IPR002957. Keratin_I.
[Graphical view]
PANTHERPTHR23239. PTHR23239. 1 hit.
PfamPF00038. Filament. 1 hit.
[Graphical view]
PRINTSPR01248. TYPE1KERATIN.
PROSITEPS00226. IF. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

GeneWikiKeratin_20.
GenomeRNAi54474.
NextBio56776.
PROP35900.
SOURCESearch...

Entry information

Entry nameK1C20_HUMAN
AccessionPrimary (citable) accession number: P35900
Secondary accession number(s): B2R6W7
Entry history
Integrated into UniProtKB/Swiss-Prot: June 1, 1994
Last sequence update: June 1, 1994
Last modified: March 19, 2014
This is version 132 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Relevant documents

SIMILARITY comments

Index of protein domains and families

MIM cross-references

Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot

Human polymorphisms and disease mutations

Index of human polymorphisms and disease mutations

Human entries with polymorphisms or disease mutations

List of human entries with polymorphisms or disease mutations

Human chromosome 17

Human chromosome 17: entries, gene names and cross-references to MIM