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Protein

Keratin, type I cytoskeletal 20

Gene

KRT20

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Plays a significant role in maintaining keratin filament organization in intestinal epithelia. When phosphorylated, plays a role in the secretion of mucin in the small intestine (By similarity).By similarity2 Publications

Miscellaneous

There are two types of cytoskeletal and microfibrillar keratin: I (acidic; 40-55 kDa) and II (neutral to basic; 56-70 kDa).

GO - Molecular functioni

  • structural constituent of cytoskeleton Source: UniProtKB

GO - Biological processi

Keywordsi

Biological processApoptosis

Enzyme and pathway databases

ReactomeiR-HSA-6805567. Keratinization.
R-HSA-6809371. Formation of the cornified envelope.

Names & Taxonomyi

Protein namesi
Recommended name:
Keratin, type I cytoskeletal 20
Alternative name(s):
Cytokeratin-20
Short name:
CK-20
Keratin-20
Short name:
K20
Protein IT
Gene namesi
Name:KRT20
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 17

Organism-specific databases

EuPathDBiHostDB:ENSG00000171431.3.
HGNCiHGNC:20412. KRT20.

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm, Intermediate filament, Keratin

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi13S → A: Promotes keratin filament disassembly. 1 Publication1
Mutagenesisi14S → A: No effect on keratin filament organization. 1 Publication1
Mutagenesisi80R → H: Leads to collapsed filaments. 1 Publication1

Organism-specific databases

DisGeNETi54474.
OpenTargetsiENSG00000171431.
PharmGKBiPA134938907.

Polymorphism and mutation databases

BioMutaiKRT20.
DMDMi547750.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000636751 – 424Keratin, type I cytoskeletal 20Add BLAST424

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei13Phosphoserine; by MAPKAPK2, MAPKAPK3 and PKC1 Publication1

Post-translational modificationi

Hyperphosphorylation at Ser-13 occurs during the early stages of apoptosis but becomes less prominent during the later stages. Phosphorylation at Ser-13 also increases in response to stress brought on by cell injury (By similarity).By similarity
Proteolytically cleaved by caspases during apoptosis. Cleavage occurs at Asp-228.1 Publication

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sitei228 – 229Cleavage; by caspases2

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiP35900.
PeptideAtlasiP35900.
PRIDEiP35900.

PTM databases

iPTMnetiP35900.
PhosphoSitePlusiP35900.
SwissPalmiP35900.

Expressioni

Tissue specificityi

Expressed predominantly in the intestinal epithelium. Expressed in luminal cells of colonic mucosa. Also expressed in the Merkel cells of keratinized oral mucosa; specifically at the tips of some rete ridges of the gingival mucosa, in the basal layer of the palatal mucosa and in the taste buds of lingual mucosa.2 Publications

Developmental stagei

First detected at embryonic week 8 in individual 'converted' simple epithelial cells of the developing intestinal mucosa. In later fetal stages, synthesis extends over most goblet cells and a variable number of villus enterocytes. In the developing gastric and intestinal mucosa, expressed in all enterocytes and goblet cells as well as certain 'low-differentiated' columnar cells, whereas the neuroendocrine and Paneth cells are negative.1 Publication

Gene expression databases

BgeeiENSG00000171431.
CleanExiHS_KRT20.
GenevisibleiP35900. HS.

Organism-specific databases

HPAiCAB000032.
HPA024309.
HPA024684.
HPA027236.

Interactioni

Subunit structurei

Heterotetramer of two type I and two type II keratins. Associates with KRT8.

Binary interactionsi

Show more details

Protein-protein interaction databases

BioGridi119979. 28 interactors.
IntActiP35900. 43 interactors.
MINTiMINT-1445560.
STRINGi9606.ENSP00000167588.

Structurei

3D structure databases

ProteinModelPortaliP35900.
SMRiP35900.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini70 – 381IF rodPROSITE-ProRule annotationAdd BLAST312

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni1 – 69HeadAdd BLAST69
Regioni70 – 105Coil 1AAdd BLAST36
Regioni106 – 123Linker 1Add BLAST18
Regioni124 – 215Coil 1BAdd BLAST92
Regioni216 – 238Linker 12Add BLAST23
Regioni239 – 377Coil 2Add BLAST139
Regioni378 – 424TailAdd BLAST47

Sequence similaritiesi

Belongs to the intermediate filament family.PROSITE-ProRule annotation

Keywords - Domaini

Coiled coil

Phylogenomic databases

eggNOGiENOG410IVBB. Eukaryota.
ENOG410YGAC. LUCA.
GeneTreeiENSGT00900000140890.
HOGENOMiHOG000230975.
HOVERGENiHBG013015.
InParanoidiP35900.
KOiK07604.
OMAiKYETERG.
OrthoDBiEOG091G087I.
PhylomeDBiP35900.
TreeFamiTF332742.

Family and domain databases

InterProiView protein in InterPro
IPR001664. IF.
IPR018039. Intermediate_filament_CS.
IPR002957. Keratin_I.
PANTHERiPTHR23239. PTHR23239. 1 hit.
PfamiView protein in Pfam
PF00038. Filament. 1 hit.
PRINTSiPR01248. TYPE1KERATIN.
SMARTiView protein in SMART
SM01391. Filament. 1 hit.
PROSITEiView protein in PROSITE
PS00226. IF_ROD_1. 1 hit.
PS51842. IF_ROD_2. 1 hit.

Sequencei

Sequence statusi: Complete.

P35900-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MDFSRRSFHR SLSSSLQAPV VSTVGMQRLG TTPSVYGGAG GRGIRISNSR
60 70 80 90 100
HTVNYGSDLT GGGDLFVGNE KMAMQNLNDR LASYLEKVRT LEQSNSKLEV
110 120 130 140 150
QIKQWYETNA PRAGRDYSAY YRQIEELRSQ IKDAQLQNAR CVLQIDNAKL
160 170 180 190 200
AAEDFRLKYE TERGIRLTVE ADLQGLNKVF DDLTLHKTDL EIQIEELNKD
210 220 230 240 250
LALLKKEHQE EVDGLHKHLG NTVNVEVDAA PGLNLGVIMN EMRQKYEVMA
260 270 280 290 300
QKNLQEAKEQ FERQTAVLQQ QVTVNTEELK GTEVQLTELR RTSQSLEIEL
310 320 330 340 350
QSHLSMKESL EHTLEETKAR YSSQLANLQS LLSSLEAQLM QIRSNMERQN
360 370 380 390 400
NEYHILLDIK TRLEQEIATY RRLLEGEDVK TTEYQLSTLE ERDIKKTRKI
410 420
KTVVQEVVDG KVVSSEVKEV EENI
Length:424
Mass (Da):48,487
Last modified:June 1, 1994 - v1
Checksum:iA8EF5A518C73CCE5
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti161T → S in CAA51914 (PubMed:7689500).Curated1
Sequence conflicti349Q → P in CAA51914 (PubMed:7689500).Curated1
Sequence conflicti398R → T in CAA51914 (PubMed:7689500).Curated1

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural variantiVAR_0363674S → R in a colorectal cancer sample; somatic mutation. 1 Publication1
Natural variantiVAR_024489129S → N1 PublicationCorresponds to variant dbSNP:rs7212483Ensembl.1
Natural variantiVAR_064726214G → C Found in a renal cell carcinoma sample; somatic mutation. 1 Publication1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X73501 Genomic DNA. Translation: CAA51913.1.
AK312744 mRNA. Translation: BAG35614.1.
BC031559 mRNA. Translation: AAH31559.1.
X73502 mRNA. Translation: CAA51914.1.
CCDSiCCDS11379.1.
PIRiS37780.
RefSeqiNP_061883.1. NM_019010.2.
UniGeneiHs.84905.

Genome annotation databases

EnsembliENST00000167588; ENSP00000167588; ENSG00000171431.
ENST00000576098; ENSP00000460501; ENSG00000263057.
GeneIDi54474.
KEGGihsa:54474.
UCSCiuc002hvl.4. human.

Keywords - Coding sequence diversityi

Polymorphism

Similar proteinsi

Entry informationi

Entry nameiK1C20_HUMAN
AccessioniPrimary (citable) accession number: P35900
Secondary accession number(s): B2R6W7
Entry historyiIntegrated into UniProtKB/Swiss-Prot: June 1, 1994
Last sequence update: June 1, 1994
Last modified: October 25, 2017
This is version 160 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Human chromosome 17
    Human chromosome 17: entries, gene names and cross-references to MIM
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  5. SIMILARITY comments
    Index of protein domains and families