Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Basket 0
(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

P35900

- K1C20_HUMAN

UniProt

P35900 - K1C20_HUMAN

Protein

Keratin, type I cytoskeletal 20

Gene

KRT20

Organism
Homo sapiens (Human)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
    • BLAST
    • Align
    • Format
    • Add to basket
    • History
      Entry version 136 (01 Oct 2014)
      Sequence version 1 (01 Jun 1994)
      Previous versions | rss
    • Help video
    • Feedback
    • Comment

    Functioni

    Plays a significant role in maintaining keratin filament organization in intestinal epithelia. When phosphorylated, plays a role in the secretion of mucin in the small intestine By similarity.By similarity

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sitei228 – 2292Cleavage; by caspases

    GO - Molecular functioni

    1. protein binding Source: UniProtKB
    2. structural constituent of cytoskeleton Source: UniProtKB

    GO - Biological processi

    1. apoptotic process Source: UniProtKB-KW
    2. cellular response to stress Source: Ensembl
    3. intermediate filament organization Source: UniProtKB
    4. regulation of protein secretion Source: Ensembl

    Keywords - Biological processi

    Apoptosis

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Keratin, type I cytoskeletal 20
    Alternative name(s):
    Cytokeratin-20
    Short name:
    CK-20
    Keratin-20
    Short name:
    K20
    Protein IT
    Gene namesi
    Name:KRT20
    OrganismiHomo sapiens (Human)
    Taxonomic identifieri9606 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
    ProteomesiUP000005640: Chromosome 17

    Organism-specific databases

    HGNCiHGNC:20412. KRT20.

    Subcellular locationi

    Cytoplasm 2 Publications

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB
    2. intermediate filament Source: UniProtKB

    Keywords - Cellular componenti

    Cytoplasm, Intermediate filament, Keratin

    Pathology & Biotechi

    Mutagenesis

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Mutagenesisi13 – 131S → A: Promotes keratin filament disassembly. 1 Publication
    Mutagenesisi14 – 141S → A: No effect on keratin filament organization. 1 Publication
    Mutagenesisi80 – 801R → H: Leads to collapsed filaments. 1 Publication

    Organism-specific databases

    PharmGKBiPA134938907.

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 424424Keratin, type I cytoskeletal 20PRO_0000063675Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei13 – 131Phosphoserine; by MAPKAPK2, MAPKAPK3 and PKC1 Publication

    Post-translational modificationi

    Hyperphosphorylation at Ser-13 occurs during the early stages of apoptosis but becomes less prominent during the later stages. Phosphorylation at Ser-13 also increases in response to stress brought on by cell injury By similarity.By similarity
    Proteolytically cleaved by caspases during apoptosis. Cleavage occurs at Asp-228.1 Publication

    Keywords - PTMi

    Phosphoprotein

    Proteomic databases

    MaxQBiP35900.
    PaxDbiP35900.
    PRIDEiP35900.

    PTM databases

    PhosphoSiteiP35900.

    Expressioni

    Tissue specificityi

    Expressed predominantly in the intestinal epithelium. Expressed in luminal cells of colonic mucosa. Also expressed in the Merkel cells of keratinized oral mucosa; specifically at the tips of some rete ridges of the gingival mucosa, in the basal layer of the palatal mucosa and in the taste buds of lingual mucosa.2 Publications

    Developmental stagei

    First detected at embryonic week 8 in individual 'converted' simple epithelial cells of the developing intestinal mucosa. In later fetal stages, synthesis extends over most goblet cells and a variable number of villus enterocytes. In the developing gastric and intestinal mucosa, expressed in all enterocytes and goblet cells as well as certain 'low-differentiated' columnar cells, whereas the neuroendocrine and Paneth cells are negative.1 Publication

    Gene expression databases

    BgeeiP35900.
    CleanExiHS_KRT20.
    GenevestigatoriP35900.

    Organism-specific databases

    HPAiCAB000032.
    HPA024309.
    HPA024684.
    HPA027236.

    Interactioni

    Subunit structurei

    Heterotetramer of two type I and two type II keratins. Associates with KRT8.

    Protein-protein interaction databases

    BioGridi119979. 17 interactions.
    IntActiP35900. 16 interactions.
    MINTiMINT-1445560.
    STRINGi9606.ENSP00000167588.

    Structurei

    3D structure databases

    ProteinModelPortaliP35900.
    SMRiP35900. Positions 287-376.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni1 – 6969HeadAdd
    BLAST
    Regioni70 – 377308RodAdd
    BLAST
    Regioni70 – 10536Coil 1AAdd
    BLAST
    Regioni106 – 12318Linker 1Add
    BLAST
    Regioni124 – 21592Coil 1BAdd
    BLAST
    Regioni216 – 23823Linker 12Add
    BLAST
    Regioni239 – 377139Coil 2Add
    BLAST
    Regioni378 – 42447TailAdd
    BLAST

    Sequence similaritiesi

    Belongs to the intermediate filament family.Curated

    Keywords - Domaini

    Coiled coil

    Phylogenomic databases

    eggNOGiNOG149615.
    HOGENOMiHOG000230975.
    HOVERGENiHBG013015.
    InParanoidiP35900.
    KOiK07604.
    OMAiQIKQWYE.
    OrthoDBiEOG7FV3Q8.
    PhylomeDBiP35900.
    TreeFamiTF332742.

    Family and domain databases

    InterProiIPR001664. IF.
    IPR018039. Intermediate_filament_CS.
    IPR002957. Keratin_I.
    [Graphical view]
    PANTHERiPTHR23239. PTHR23239. 1 hit.
    PfamiPF00038. Filament. 1 hit.
    [Graphical view]
    PRINTSiPR01248. TYPE1KERATIN.
    PROSITEiPS00226. IF. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P35900-1 [UniParc]FASTAAdd to Basket

    « Hide

    MDFSRRSFHR SLSSSLQAPV VSTVGMQRLG TTPSVYGGAG GRGIRISNSR    50
    HTVNYGSDLT GGGDLFVGNE KMAMQNLNDR LASYLEKVRT LEQSNSKLEV 100
    QIKQWYETNA PRAGRDYSAY YRQIEELRSQ IKDAQLQNAR CVLQIDNAKL 150
    AAEDFRLKYE TERGIRLTVE ADLQGLNKVF DDLTLHKTDL EIQIEELNKD 200
    LALLKKEHQE EVDGLHKHLG NTVNVEVDAA PGLNLGVIMN EMRQKYEVMA 250
    QKNLQEAKEQ FERQTAVLQQ QVTVNTEELK GTEVQLTELR RTSQSLEIEL 300
    QSHLSMKESL EHTLEETKAR YSSQLANLQS LLSSLEAQLM QIRSNMERQN 350
    NEYHILLDIK TRLEQEIATY RRLLEGEDVK TTEYQLSTLE ERDIKKTRKI 400
    KTVVQEVVDG KVVSSEVKEV EENI 424
    Length:424
    Mass (Da):48,487
    Last modified:June 1, 1994 - v1
    Checksum:iA8EF5A518C73CCE5
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti161 – 1611T → S in CAA51914. (PubMed:7689500)Curated
    Sequence conflicti349 – 3491Q → P in CAA51914. (PubMed:7689500)Curated
    Sequence conflicti398 – 3981R → T in CAA51914. (PubMed:7689500)Curated

    Natural variant

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Natural varianti4 – 41S → R in a colorectal cancer sample; somatic mutation. 1 Publication
    VAR_036367
    Natural varianti129 – 1291S → N.1 Publication
    Corresponds to variant rs7212483 [ dbSNP | Ensembl ].
    VAR_024489
    Natural varianti214 – 2141G → C Found in a renal cell carcinoma sample; somatic mutation. 1 Publication
    VAR_064726

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X73501 Genomic DNA. Translation: CAA51913.1.
    AK312744 mRNA. Translation: BAG35614.1.
    BC031559 mRNA. Translation: AAH31559.1.
    X73502 mRNA. Translation: CAA51914.1.
    CCDSiCCDS11379.1.
    PIRiS37780.
    RefSeqiNP_061883.1. NM_019010.2.
    UniGeneiHs.84905.

    Genome annotation databases

    EnsembliENST00000167588; ENSP00000167588; ENSG00000171431.
    ENST00000576098; ENSP00000460501; ENSG00000263057.
    GeneIDi54474.
    KEGGihsa:54474.
    UCSCiuc002hvl.3. human.

    Polymorphism databases

    DMDMi547750.

    Keywords - Coding sequence diversityi

    Polymorphism

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X73501 Genomic DNA. Translation: CAA51913.1 .
    AK312744 mRNA. Translation: BAG35614.1 .
    BC031559 mRNA. Translation: AAH31559.1 .
    X73502 mRNA. Translation: CAA51914.1 .
    CCDSi CCDS11379.1.
    PIRi S37780.
    RefSeqi NP_061883.1. NM_019010.2.
    UniGenei Hs.84905.

    3D structure databases

    ProteinModelPortali P35900.
    SMRi P35900. Positions 287-376.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 119979. 17 interactions.
    IntActi P35900. 16 interactions.
    MINTi MINT-1445560.
    STRINGi 9606.ENSP00000167588.

    PTM databases

    PhosphoSitei P35900.

    Polymorphism databases

    DMDMi 547750.

    Proteomic databases

    MaxQBi P35900.
    PaxDbi P35900.
    PRIDEi P35900.

    Protocols and materials databases

    DNASUi 54474.
    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENST00000167588 ; ENSP00000167588 ; ENSG00000171431 .
    ENST00000576098 ; ENSP00000460501 ; ENSG00000263057 .
    GeneIDi 54474.
    KEGGi hsa:54474.
    UCSCi uc002hvl.3. human.

    Organism-specific databases

    CTDi 54474.
    GeneCardsi GC17M039030.
    HGNCi HGNC:20412. KRT20.
    HPAi CAB000032.
    HPA024309.
    HPA024684.
    HPA027236.
    MIMi 608218. gene.
    neXtProti NX_P35900.
    PharmGKBi PA134938907.
    GenAtlasi Search...

    Phylogenomic databases

    eggNOGi NOG149615.
    HOGENOMi HOG000230975.
    HOVERGENi HBG013015.
    InParanoidi P35900.
    KOi K07604.
    OMAi QIKQWYE.
    OrthoDBi EOG7FV3Q8.
    PhylomeDBi P35900.
    TreeFami TF332742.

    Miscellaneous databases

    GeneWikii Keratin_20.
    GenomeRNAii 54474.
    NextBioi 56776.
    PROi P35900.
    SOURCEi Search...

    Gene expression databases

    Bgeei P35900.
    CleanExi HS_KRT20.
    Genevestigatori P35900.

    Family and domain databases

    InterProi IPR001664. IF.
    IPR018039. Intermediate_filament_CS.
    IPR002957. Keratin_I.
    [Graphical view ]
    PANTHERi PTHR23239. PTHR23239. 1 hit.
    Pfami PF00038. Filament. 1 hit.
    [Graphical view ]
    PRINTSi PR01248. TYPE1KERATIN.
    PROSITEi PS00226. IF. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "The human gene encoding cytokeratin 20 and its expression during fetal development and in gastrointestinal carcinomas."
      Moll R., Zimbelmann R., Goldschmidt M.D., Keith M., Laufer J., Kasper M., Koch P.J., Franke W.W.
      Differentiation 53:75-93(1993) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], DEVELOPMENTAL STAGE.
      Tissue: Intestine.
    2. "Complete sequencing and characterization of 21,243 full-length human cDNAs."
      Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S.
      , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S.
      Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA], VARIANT ASN-129.
      Tissue: Small intestine.
    3. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    4. "Keratin 20 serine 13 phosphorylation is a stress and intestinal goblet cell marker."
      Zhou Q., Cadrin M., Herrmann H., Chen C.-H., Chalkley R.J., Burlingame A.L., Omary M.B.
      J. Biol. Chem. 281:16453-16461(2006) [PubMed] [Europe PMC] [Abstract]
      Cited for: PROTEIN SEQUENCE OF 11-28, FUNCTION, INTERACTION WITH KRT8, PHOSPHORYLATION AT SER-13, CLEAVAGE AT ASP-228, MUTAGENESIS OF SER-13 AND SER-14.
    5. "Differential localization by in situ hybridization of distinct keratin mRNA species during intestinal epithelial cell development and differentiation."
      Calnek D., Quaroni A.
      Differentiation 53:95-104(1993) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 86-424.
      Tissue: Intestine.
    6. "Identification of protein IT of the intestinal cytoskeleton as a novel type I cytokeratin with unusual properties and expression patterns."
      Moll R., Schiller D.L., Franke W.W.
      J. Cell Biol. 111:567-580(1990) [PubMed] [Europe PMC] [Abstract]
      Cited for: IDENTIFICATION, SUBCELLULAR LOCATION, TISSUE SPECIFICITY.
    7. "An immunohistological study of cytokeratin 20 in human and mammalian oral epithelium."
      Barrett A.W., Cort E.M., Patel P., Berkovitz B.K.B.
      Arch. Oral Biol. 45:879-887(2000) [PubMed] [Europe PMC] [Abstract]
      Cited for: INTERACTION WITH KRT8, SUBCELLULAR LOCATION, TISSUE SPECIFICITY.
    8. "Keratin 20 helps maintain intermediate filament organization in intestinal epithelia."
      Zhou Q., Toivola D.M., Feng N., Greenberg H.B., Franke W.W., Omary M.B.
      Mol. Biol. Cell 14:2959-2971(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, MUTAGENESIS OF ARG-80.
    9. Cited for: VARIANT [LARGE SCALE ANALYSIS] ARG-4.
    10. Cited for: VARIANT CYS-214.

    Entry informationi

    Entry nameiK1C20_HUMAN
    AccessioniPrimary (citable) accession number: P35900
    Secondary accession number(s): B2R6W7
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: June 1, 1994
    Last sequence update: June 1, 1994
    Last modified: October 1, 2014
    This is version 136 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program
    DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

    Miscellaneousi

    Miscellaneous

    There are two types of cytoskeletal and microfibrillar keratin: I (acidic; 40-55 kDa) and II (neutral to basic; 56-70 kDa).

    Keywords - Technical termi

    Complete proteome, Direct protein sequencing, Reference proteome

    Documents

    1. Human chromosome 17
      Human chromosome 17: entries, gene names and cross-references to MIM
    2. Human entries with polymorphisms or disease mutations
      List of human entries with polymorphisms or disease mutations
    3. Human polymorphisms and disease mutations
      Index of human polymorphisms and disease mutations
    4. MIM cross-references
      Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
    5. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3