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Protein

Keratin, type I cytoskeletal 20

Gene

KRT20

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Plays a significant role in maintaining keratin filament organization in intestinal epithelia. When phosphorylated, plays a role in the secretion of mucin in the small intestine (By similarity).By similarity

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sitei228 – 2292Cleavage; by caspases

GO - Molecular functioni

  • structural constituent of cytoskeleton Source: UniProtKB

GO - Biological processi

  • apoptotic process Source: UniProtKB-KW
  • cellular response to stress Source: Ensembl
  • intermediate filament organization Source: UniProtKB
  • regulation of protein secretion Source: Ensembl
Complete GO annotation...

Keywords - Biological processi

Apoptosis

Names & Taxonomyi

Protein namesi
Recommended name:
Keratin, type I cytoskeletal 20
Alternative name(s):
Cytokeratin-20
Short name:
CK-20
Keratin-20
Short name:
K20
Protein IT
Gene namesi
Name:KRT20
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
ProteomesiUP000005640 Componentsi: Chromosome 17, Unplaced

Organism-specific databases

HGNCiHGNC:20412. KRT20.

Subcellular locationi

GO - Cellular componenti

  • cytoplasm Source: UniProtKB
  • intermediate filament Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Intermediate filament, Keratin

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi13 – 131S → A: Promotes keratin filament disassembly. 1 Publication
Mutagenesisi14 – 141S → A: No effect on keratin filament organization. 1 Publication
Mutagenesisi80 – 801R → H: Leads to collapsed filaments. 1 Publication

Organism-specific databases

PharmGKBiPA134938907.

Polymorphism and mutation databases

BioMutaiKRT20.
DMDMi547750.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 424424Keratin, type I cytoskeletal 20PRO_0000063675Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei13 – 131Phosphoserine; by MAPKAPK2, MAPKAPK3 and PKC1 Publication

Post-translational modificationi

Hyperphosphorylation at Ser-13 occurs during the early stages of apoptosis but becomes less prominent during the later stages. Phosphorylation at Ser-13 also increases in response to stress brought on by cell injury (By similarity).By similarity
Proteolytically cleaved by caspases during apoptosis. Cleavage occurs at Asp-228.1 Publication

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiP35900.
PaxDbiP35900.
PRIDEiP35900.

PTM databases

PhosphoSiteiP35900.

Expressioni

Tissue specificityi

Expressed predominantly in the intestinal epithelium. Expressed in luminal cells of colonic mucosa. Also expressed in the Merkel cells of keratinized oral mucosa; specifically at the tips of some rete ridges of the gingival mucosa, in the basal layer of the palatal mucosa and in the taste buds of lingual mucosa.2 Publications

Developmental stagei

First detected at embryonic week 8 in individual 'converted' simple epithelial cells of the developing intestinal mucosa. In later fetal stages, synthesis extends over most goblet cells and a variable number of villus enterocytes. In the developing gastric and intestinal mucosa, expressed in all enterocytes and goblet cells as well as certain 'low-differentiated' columnar cells, whereas the neuroendocrine and Paneth cells are negative.1 Publication

Gene expression databases

BgeeiP35900.
CleanExiHS_KRT20.
GenevestigatoriP35900.

Organism-specific databases

HPAiCAB000032.
HPA024309.
HPA024684.
HPA027236.

Interactioni

Subunit structurei

Heterotetramer of two type I and two type II keratins. Associates with KRT8.

Binary interactionsi

WithEntry#Exp.IntActNotes
AMOTL2Q9Y2J4-43EBI-742094,EBI-10187270
C9orf16Q9BUW76EBI-742094,EBI-752084
KRT13A1A4E93EBI-742094,EBI-10171552
KRT15P190124EBI-742094,EBI-739566
KRT40Q6A1623EBI-742094,EBI-10171697
KRT80Q6KB663EBI-742094,EBI-3046635
KRTAP10-7P604093EBI-742094,EBI-10172290
KRTAP10-8P604103EBI-742094,EBI-10171774
KRTAP10-9P604113EBI-742094,EBI-10172052
NOTCH2NLQ7Z3S93EBI-742094,EBI-945833
NUP62P371983EBI-742094,EBI-347978
PIK3R2O004593EBI-742094,EBI-346930
TFIP11Q9UBB94EBI-742094,EBI-1105213
TXLNAP402223EBI-742094,EBI-359793
USHBP1Q8N6Y03EBI-742094,EBI-739895
VIMP086704EBI-742094,EBI-353844

Protein-protein interaction databases

BioGridi119979. 29 interactions.
IntActiP35900. 27 interactions.
MINTiMINT-1445560.
STRINGi9606.ENSP00000167588.

Structurei

3D structure databases

ProteinModelPortaliP35900.
SMRiP35900. Positions 287-376.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni1 – 6969HeadAdd
BLAST
Regioni70 – 377308RodAdd
BLAST
Regioni70 – 10536Coil 1AAdd
BLAST
Regioni106 – 12318Linker 1Add
BLAST
Regioni124 – 21592Coil 1BAdd
BLAST
Regioni216 – 23823Linker 12Add
BLAST
Regioni239 – 377139Coil 2Add
BLAST
Regioni378 – 42447TailAdd
BLAST

Sequence similaritiesi

Belongs to the intermediate filament family.Curated

Keywords - Domaini

Coiled coil

Phylogenomic databases

eggNOGiNOG149615.
GeneTreeiENSGT00760000118808.
HOGENOMiHOG000230975.
HOVERGENiHBG013015.
InParanoidiP35900.
KOiK07604.
OMAiQIKQWYE.
OrthoDBiEOG7FV3Q8.
PhylomeDBiP35900.
TreeFamiTF332742.

Family and domain databases

InterProiIPR001664. IF.
IPR018039. Intermediate_filament_CS.
IPR002957. Keratin_I.
[Graphical view]
PANTHERiPTHR23239. PTHR23239. 1 hit.
PfamiPF00038. Filament. 1 hit.
[Graphical view]
PRINTSiPR01248. TYPE1KERATIN.
PROSITEiPS00226. IF. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P35900-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MDFSRRSFHR SLSSSLQAPV VSTVGMQRLG TTPSVYGGAG GRGIRISNSR
60 70 80 90 100
HTVNYGSDLT GGGDLFVGNE KMAMQNLNDR LASYLEKVRT LEQSNSKLEV
110 120 130 140 150
QIKQWYETNA PRAGRDYSAY YRQIEELRSQ IKDAQLQNAR CVLQIDNAKL
160 170 180 190 200
AAEDFRLKYE TERGIRLTVE ADLQGLNKVF DDLTLHKTDL EIQIEELNKD
210 220 230 240 250
LALLKKEHQE EVDGLHKHLG NTVNVEVDAA PGLNLGVIMN EMRQKYEVMA
260 270 280 290 300
QKNLQEAKEQ FERQTAVLQQ QVTVNTEELK GTEVQLTELR RTSQSLEIEL
310 320 330 340 350
QSHLSMKESL EHTLEETKAR YSSQLANLQS LLSSLEAQLM QIRSNMERQN
360 370 380 390 400
NEYHILLDIK TRLEQEIATY RRLLEGEDVK TTEYQLSTLE ERDIKKTRKI
410 420
KTVVQEVVDG KVVSSEVKEV EENI
Length:424
Mass (Da):48,487
Last modified:June 1, 1994 - v1
Checksum:iA8EF5A518C73CCE5
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti161 – 1611T → S in CAA51914 (PubMed:7689500).Curated
Sequence conflicti349 – 3491Q → P in CAA51914 (PubMed:7689500).Curated
Sequence conflicti398 – 3981R → T in CAA51914 (PubMed:7689500).Curated

Natural variant

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Natural varianti4 – 41S → R in a colorectal cancer sample; somatic mutation. 1 Publication
VAR_036367
Natural varianti129 – 1291S → N.1 Publication
Corresponds to variant rs7212483 [ dbSNP | Ensembl ].
VAR_024489
Natural varianti214 – 2141G → C Found in a renal cell carcinoma sample; somatic mutation. 1 Publication
VAR_064726

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X73501 Genomic DNA. Translation: CAA51913.1.
AK312744 mRNA. Translation: BAG35614.1.
BC031559 mRNA. Translation: AAH31559.1.
X73502 mRNA. Translation: CAA51914.1.
CCDSiCCDS11379.1.
PIRiS37780.
RefSeqiNP_061883.1. NM_019010.2.
UniGeneiHs.84905.

Genome annotation databases

EnsembliENST00000167588; ENSP00000167588; ENSG00000171431.
ENST00000576098; ENSP00000460501; ENSG00000263057.
GeneIDi54474.
KEGGihsa:54474.
UCSCiuc002hvl.3. human.

Keywords - Coding sequence diversityi

Polymorphism

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X73501 Genomic DNA. Translation: CAA51913.1.
AK312744 mRNA. Translation: BAG35614.1.
BC031559 mRNA. Translation: AAH31559.1.
X73502 mRNA. Translation: CAA51914.1.
CCDSiCCDS11379.1.
PIRiS37780.
RefSeqiNP_061883.1. NM_019010.2.
UniGeneiHs.84905.

3D structure databases

ProteinModelPortaliP35900.
SMRiP35900. Positions 287-376.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi119979. 29 interactions.
IntActiP35900. 27 interactions.
MINTiMINT-1445560.
STRINGi9606.ENSP00000167588.

PTM databases

PhosphoSiteiP35900.

Polymorphism and mutation databases

BioMutaiKRT20.
DMDMi547750.

Proteomic databases

MaxQBiP35900.
PaxDbiP35900.
PRIDEiP35900.

Protocols and materials databases

DNASUi54474.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000167588; ENSP00000167588; ENSG00000171431.
ENST00000576098; ENSP00000460501; ENSG00000263057.
GeneIDi54474.
KEGGihsa:54474.
UCSCiuc002hvl.3. human.

Organism-specific databases

CTDi54474.
GeneCardsiGC17M039030.
HGNCiHGNC:20412. KRT20.
HPAiCAB000032.
HPA024309.
HPA024684.
HPA027236.
MIMi608218. gene.
neXtProtiNX_P35900.
PharmGKBiPA134938907.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiNOG149615.
GeneTreeiENSGT00760000118808.
HOGENOMiHOG000230975.
HOVERGENiHBG013015.
InParanoidiP35900.
KOiK07604.
OMAiQIKQWYE.
OrthoDBiEOG7FV3Q8.
PhylomeDBiP35900.
TreeFamiTF332742.

Miscellaneous databases

GeneWikiiKeratin_20.
GenomeRNAii54474.
NextBioi56776.
PROiP35900.
SOURCEiSearch...

Gene expression databases

BgeeiP35900.
CleanExiHS_KRT20.
GenevestigatoriP35900.

Family and domain databases

InterProiIPR001664. IF.
IPR018039. Intermediate_filament_CS.
IPR002957. Keratin_I.
[Graphical view]
PANTHERiPTHR23239. PTHR23239. 1 hit.
PfamiPF00038. Filament. 1 hit.
[Graphical view]
PRINTSiPR01248. TYPE1KERATIN.
PROSITEiPS00226. IF. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "The human gene encoding cytokeratin 20 and its expression during fetal development and in gastrointestinal carcinomas."
    Moll R., Zimbelmann R., Goldschmidt M.D., Keith M., Laufer J., Kasper M., Koch P.J., Franke W.W.
    Differentiation 53:75-93(1993) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], DEVELOPMENTAL STAGE.
    Tissue: Intestine.
  2. "Complete sequencing and characterization of 21,243 full-length human cDNAs."
    Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S.
    , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S.
    Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA], VARIANT ASN-129.
    Tissue: Small intestine.
  3. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
  4. "Keratin 20 serine 13 phosphorylation is a stress and intestinal goblet cell marker."
    Zhou Q., Cadrin M., Herrmann H., Chen C.-H., Chalkley R.J., Burlingame A.L., Omary M.B.
    J. Biol. Chem. 281:16453-16461(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: PROTEIN SEQUENCE OF 11-28, FUNCTION, INTERACTION WITH KRT8, PHOSPHORYLATION AT SER-13, CLEAVAGE AT ASP-228, MUTAGENESIS OF SER-13 AND SER-14.
  5. "Differential localization by in situ hybridization of distinct keratin mRNA species during intestinal epithelial cell development and differentiation."
    Calnek D., Quaroni A.
    Differentiation 53:95-104(1993) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 86-424.
    Tissue: Intestine.
  6. "Identification of protein IT of the intestinal cytoskeleton as a novel type I cytokeratin with unusual properties and expression patterns."
    Moll R., Schiller D.L., Franke W.W.
    J. Cell Biol. 111:567-580(1990) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION, SUBCELLULAR LOCATION, TISSUE SPECIFICITY.
  7. "An immunohistological study of cytokeratin 20 in human and mammalian oral epithelium."
    Barrett A.W., Cort E.M., Patel P., Berkovitz B.K.B.
    Arch. Oral Biol. 45:879-887(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH KRT8, SUBCELLULAR LOCATION, TISSUE SPECIFICITY.
  8. "Keratin 20 helps maintain intermediate filament organization in intestinal epithelia."
    Zhou Q., Toivola D.M., Feng N., Greenberg H.B., Franke W.W., Omary M.B.
    Mol. Biol. Cell 14:2959-2971(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, MUTAGENESIS OF ARG-80.
  9. Cited for: VARIANT [LARGE SCALE ANALYSIS] ARG-4.
  10. Cited for: VARIANT CYS-214.

Entry informationi

Entry nameiK1C20_HUMAN
AccessioniPrimary (citable) accession number: P35900
Secondary accession number(s): B2R6W7
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 1, 1994
Last sequence update: June 1, 1994
Last modified: April 29, 2015
This is version 141 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Miscellaneous

There are two types of cytoskeletal and microfibrillar keratin: I (acidic; 40-55 kDa) and II (neutral to basic; 56-70 kDa).

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Human chromosome 17
    Human chromosome 17: entries, gene names and cross-references to MIM
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  5. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.