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Protein

Chromosomal replication initiator protein DnaA

Gene

dnaA

Organism
Mycoplasma genitalium (strain ATCC 33530 / G-37 / NCTC 10195)
Status
Reviewed-Annotation score: Annotation score: 2 out of 5-Experimental evidence at protein leveli

Functioni

Plays an important role in the initiation and regulation of chromosomal replication. Binds to the origin of replication; it binds specifically double-stranded DNA at a 9 bp consensus (dnaA box): 5'-TTATC[CA]A[CA]A-3'. DnaA binds to ATP and to acidic phospholipids (By similarity).By similarity

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi141 – 148ATPSequence analysis8

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Biological processi

DNA replication

Keywords - Ligandi

ATP-binding, DNA-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Chromosomal replication initiator protein DnaA
Gene namesi
Name:dnaA
Ordered Locus Names:MG469
OrganismiMycoplasma genitalium (strain ATCC 33530 / G-37 / NCTC 10195)
Taxonomic identifieri243273 [NCBI]
Taxonomic lineageiBacteriaTenericutesMollicutesMycoplasmataceaeMycoplasma
Proteomesi
  • UP000000807 Componenti: Chromosome

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001142111 – 437Chromosomal replication initiator protein DnaAAdd BLAST437

Structurei

Secondary structure

1437
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi2 – 14Combined sources13
Helixi19 – 24Combined sources6
Turni25 – 28Combined sources4
Beta strandi32 – 34Combined sources3
Beta strandi36 – 42Combined sources7
Helixi46 – 54Combined sources9
Helixi59 – 62Combined sources4
Turni63 – 65Combined sources3
Beta strandi71 – 75Combined sources5
Helixi77 – 85Combined sources9

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2JMPNMR-A1-100[»]
ProteinModelPortaliP35888.
SMRiP35888.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP35888.

Family & Domainsi

Sequence similaritiesi

Belongs to the DnaA family.Curated

Phylogenomic databases

KOiK02313.
OMAiAVGNSIM.
OrthoDBiPOG091H02FF.

Family and domain databases

CDDicd06571. Bac_DnaA_C. 1 hit.
Gene3Di1.10.1750.10. 1 hit.
3.30.300.20. 1 hit.
3.40.50.300. 1 hit.
HAMAPiMF_00377. DnaA_bact. 1 hit.
InterProiIPR003593. AAA+_ATPase.
IPR001957. Chromosome_initiator_DnaA.
IPR020591. Chromosome_initiator_DnaA-like.
IPR018312. Chromosome_initiator_DnaA_CS.
IPR013317. DnaA.
IPR013159. DnaA_C.
IPR024633. DnaA_N_dom.
IPR015946. KH_dom-like_a/b.
IPR027417. P-loop_NTPase.
IPR010921. Trp_repressor/repl_initiator.
[Graphical view]
PfamiPF00308. Bac_DnaA. 1 hit.
PF08299. Bac_DnaA_C. 1 hit.
PF11638. DnaA_N. 1 hit.
[Graphical view]
PRINTSiPR00051. DNAA.
SMARTiSM00382. AAA. 1 hit.
SM00760. Bac_DnaA_C. 1 hit.
[Graphical view]
SUPFAMiSSF48295. SSF48295. 1 hit.
SSF52540. SSF52540. 1 hit.
TIGRFAMsiTIGR00362. DnaA. 1 hit.
PROSITEiPS01008. DNAA. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P35888-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MEQFNAFKSL LKKHYEKTIG FHDKYIKDIN RFVFKNNVLL ILLENEFARN
60 70 80 90 100
SLNDNSEIIH LAESLYEGIK SVNFVNEQDF FFNLAKLEEN SRDTLYQNSG
110 120 130 140 150
LSKNYTFQNF VISEGNKRAY EAGVRLAETQ DNEFSPLFIY GETGLGKTHL
160 170 180 190 200
LQAIGNEKFR NFPNARVKYV VSSDFAQEVV DAFYQRDKGI EKLKKNYENL
210 220 230 240 250
DLVLIDDTQI FGRKEKTLEI LFNIFNNLVL NKKQIVLVSD KAPDELIDID
260 270 280 290 300
ARMISRFKSG LLLKIEKHNL SSLCEILTVK LKEKDPNIQI TNEARHDAAQ
310 320 330 340 350
ISGNDVRALN GIATKLLFFA KTSKQNLINT ENLKEILFEE FEKFHKKSFD
360 370 380 390 400
PYLLIENVCR RFNVPMDSVL SENRKAELVR VRDVCNYLLR QKYNMQFQQI
410 420 430
GKIFKRSHSS VLMAVKRVAK MIENDSSLRD VITSLVI
Length:437
Mass (Da):50,762
Last modified:February 1, 1996 - v2
Checksum:iD4DF45A0F4768ED6
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti198E → G in AAD12524 (PubMed:8253680).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L43967 Genomic DNA. Translation: AAC72490.1.
U02259 Genomic DNA. Translation: AAD12524.1.
U02145 Genomic DNA. Translation: AAD12425.1.
PIRiH64251.
RefSeqiWP_010869493.1. NC_000908.2.

Genome annotation databases

EnsemblBacteriaiAAC72490; AAC72490; MG_469.
KEGGimge:MG_469.
PATRICi20010534. VBIMycGen98045_0540.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L43967 Genomic DNA. Translation: AAC72490.1.
U02259 Genomic DNA. Translation: AAD12524.1.
U02145 Genomic DNA. Translation: AAD12425.1.
PIRiH64251.
RefSeqiWP_010869493.1. NC_000908.2.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2JMPNMR-A1-100[»]
ProteinModelPortaliP35888.
SMRiP35888.
ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC72490; AAC72490; MG_469.
KEGGimge:MG_469.
PATRICi20010534. VBIMycGen98045_0540.

Phylogenomic databases

KOiK02313.
OMAiAVGNSIM.
OrthoDBiPOG091H02FF.

Miscellaneous databases

EvolutionaryTraceiP35888.

Family and domain databases

CDDicd06571. Bac_DnaA_C. 1 hit.
Gene3Di1.10.1750.10. 1 hit.
3.30.300.20. 1 hit.
3.40.50.300. 1 hit.
HAMAPiMF_00377. DnaA_bact. 1 hit.
InterProiIPR003593. AAA+_ATPase.
IPR001957. Chromosome_initiator_DnaA.
IPR020591. Chromosome_initiator_DnaA-like.
IPR018312. Chromosome_initiator_DnaA_CS.
IPR013317. DnaA.
IPR013159. DnaA_C.
IPR024633. DnaA_N_dom.
IPR015946. KH_dom-like_a/b.
IPR027417. P-loop_NTPase.
IPR010921. Trp_repressor/repl_initiator.
[Graphical view]
PfamiPF00308. Bac_DnaA. 1 hit.
PF08299. Bac_DnaA_C. 1 hit.
PF11638. DnaA_N. 1 hit.
[Graphical view]
PRINTSiPR00051. DNAA.
SMARTiSM00382. AAA. 1 hit.
SM00760. Bac_DnaA_C. 1 hit.
[Graphical view]
SUPFAMiSSF48295. SSF48295. 1 hit.
SSF52540. SSF52540. 1 hit.
TIGRFAMsiTIGR00362. DnaA. 1 hit.
PROSITEiPS01008. DNAA. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiDNAA_MYCGE
AccessioniPrimary (citable) accession number: P35888
Secondary accession number(s): Q49363
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 1, 1994
Last sequence update: February 1, 1996
Last modified: November 2, 2016
This is version 113 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Mycoplasma genitalium
    Mycoplasma genitalium (strain G-37): entries and gene names
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.