Reviewed, UniProtKB/Swiss-Prot P35885 (GYRA_STRCO)

Names and origin

Protein names

Recommended name:
    DNA gyrase subunit A
    EC=5.99.1.3

Gene names

Name:	gyrA
Ordered Locus Names:	SCO3873
ORF Names:	SCH18.10c

Organism

Streptomyces coelicolor [Complete proteome] [HAMAP]

Taxonomic identifier

1902 [NCBI]

Taxonomic lineage

Bacteria › Actinobacteria › Actinobacteridae › Actinomycetales › Streptomycineae › Streptomycetaceae › Streptomyces

Protein attributes

Sequence length	857 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	DNA gyrase negatively supercoils closed circular double-stranded DNA in an ATP-dependent manner and also catalyzes the interconversion of other topological isomers of double-stranded DNA rings, including catenanes and knotted rings.
Catalytic activity	ATP-dependent breakage, passage and rejoining of double-stranded DNA.
Subunit structure	Made up of two chains. The A chain is responsible for DNA breakage and rejoining; the B chain catalyzes ATP hydrolysis. The enzyme forms an A2B2 tetramer.
Sequence similarities	Belongs to the topoisomerase gyrA/parC subunit family.

Ontologies

Keywords
Ligand	ATP-binding DNA-binding Nucleotide-binding
Molecular function	Isomerase Topoisomerase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	DNA topological change Inferred from electronic annotation. Source: InterPro
Cellular component	chromosome Inferred from electronic annotation. Source: InterPro
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW DNA topoisomerase (ATP-hydrolyzing) activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 857	857	DNA gyrase subunit A		PRO_0000145261
Sites
	Active site	127	1	O-(5'-phospho-DNA)-tyrosine intermediate By similarity
Experimental info
	Sequence conflict	41	1	D → V in AAA65216. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	P35885-1 [UniParc]. Last modified October 18, 2001. Version 2. Checksum: 4306FD52CE64C547	FASTA	857	94,583
	10 20 30 40 50 60 MTTPEGDALA MRVEPVGLET EMQRSYLDYA MSVIVSRALP DVRDGLKPVH RRVLYAMYDG 70 80 90 100 110 120 GYRPERGFYK CARVVGDVMG NYHPHGDSSI YDALVRLAQP WSMRMPLVDS NGNFGSPGND 130 140 150 160 170 180 PAAAMRYTEC KMAPLSMEMV RDIDEETVDF TDNYDGRSQE PTVLPARFPN LLINGSAGIA 190 200 210 220 230 240 VGMATNIPPH NLREVAAGAQ WYLENYEASH EELLDALIER IKGPDFPTGA LVVGRKGIEE 250 260 270 280 290 300 AYRTGRGSIT MRAVVEVEEI QNRQCLVVTE LPYQTNPDNL AQKIADLVKD GKVGGIADVR 310 320 330 340 350 360 DETSSRTGQR LVIVLKRDAV AKVVLNNLYK HTDLQSNFGA NMLALVDGVP RTLSLDAFIR 370 380 390 400 410 420 HWVNHQIEVI VRRTRFRLRK AEERAHILRG LLKALDAIDE VIALIRRSDT VEIARGGLMD 430 440 450 460 470 480 LLEIDEIQAN AILEMQLRRL AALERQKIVR EHDELQAKIT EYNEILASPV RQRGIVSEEL 490 500 510 520 530 540 TALVEKYGDD RKTKLIPYEG DMSIEDLIAE EDIVVTVTRG GYIKRTKTDD YRAQKRGGKG 550 560 570 580 590 600 VRGTKLKEDD IVNHFFVSTT HHWLLFFTNK GRVYRAKAYE LPDAGRDARG QHVANLLAFQ 610 620 630 640 650 660 PDETIAQIRA IRDYEAVPYL VLATKAGLVK KTPLKDYDSP RSGGVIAINL REQADGSDDE 670 680 690 700 710 720 LIGAELVSAE DDLLLISKKA QSIRFTASDD TLRPMGRATS GVKGMSFREG DELLSMNVVR 730 740 750 760 770 780 AGTFVFTATD GGYAKRTSVD EYRVQGRGGL GIKAAKIVED RGSLVGALVV EEHDEILAIT 790 800 810 820 830 840 LSGGVIRTRV NGVRETGRDT MGVQLINLGK RDAVVGIARN AEAGREAEEV DGDVAVDETA 850 EGAATTGTDE GEAPSAE

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Gene organization in the dnaA-gyrA region of the Streptomyces coelicolor chromosome." Calcutt M.J. Gene 151:23-28(1994) [PubMed: 7828880] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: A3(2).
[2]	"Complete genome sequence of the model actinomycete Streptomyces coelicolor A3(2)." Bentley S.D., Chater K.F., Cerdeno-Tarraga A.-M., Challis G.L., Thomson N.R., James K.D., Harris D.E., Quail M.A., Kieser H., Harper D., Bateman A., Brown S., Chandra G., Chen C.W., Collins M., Cronin A., Fraser A., Goble A. , Hidalgo J., Hornsby T., Howarth S., Huang C.-H., Kieser T., Larke L., Murphy L.D., Oliver K., O'Neil S., Rabbinowitsch E., Rajandream M.A., Rutherford K.M., Rutter S., Seeger K., Saunders D., Sharp S., Squares R., Squares S., Taylor K., Warren T., Wietzorrek A., Woodward J.R., Barrell B.G., Parkhill J., Hopwood D.A. Nature 417:141-147(2002) [PubMed: 12000953] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC BAA-471 / A3(2) / M145.

Cross-references

Sequence databases
EMBLGenBankDDBJ	L27063 Genomic DNA. Translation: AAA65216.1. Different initiation. AL939118 Genomic DNA. Translation: CAB92993.1.
RefSeq	NP_628060.1.
3D structure databases
HSSP	HSSP built from PDB template 1AB4 based on UniProtKB P09097.
ModBase	Search...
Genome annotation databases
GeneID	1099309.
GenomeReviews	Gene locus SCO3873 in contig AL645882_GR.
KEGG	sco:SCO3873.
NMPDR	fig|100226.1.peg.3830.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	P35885.
OMA	ELGNDWN.
Enzyme and pathway databases
BioCyc	SCOE100226:SCO3873-MON.
BRENDA	5.99.1.3. 1084.
Family and domain databases
InterPro	IPR005743. GyrA. IPR006691. GyrA/parC_pinwhl. IPR002205. Topo_IIA_A/C. IPR013758. Topo_IIA_A/C_ab. IPR013757. Topo_IIA_A_a. [Graphical view]
Gene3D	G3DSA:3.90.199.10. Topo_IIA_A/C_ab. 1 hit. G3DSA:1.10.268.10. Topo_IIA_A_a. 1 hit.
Pfam	PF03989. DNA_gyraseA_C. 6 hits. PF00521. DNA_topoisoIV. 1 hit. [Graphical view]
ProDom	PD000742. DNA_topoisoIV. 1 hit. [Graphical view] [Entries sharing at least one domain]
SMART	SM00434. TOP4c. 1 hit. [Graphical view]
TIGRFAMs	TIGR01063. gyrA. 1 hit.
ProtoNet	Search...

Entry information

Entry name

GYRA_STRCO

Accession

Primary (citable) accession number: P35885
Secondary accession number(s): Q9KXX5

Entry history

Integrated into UniProtKB/Swiss-Prot:	June 1, 1994
Last sequence update:	October 18, 2001
Last modified:	November 3, 2009
This is version 60 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)