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Protein

Poly [ADP-ribose] polymerase

Gene

Parp

Organism
Drosophila melanogaster (Fruit fly)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Poly[ADP-ribose] polymerase modifies various nuclear proteins by poly(ADP-ribosyl)ation. The modification is dependent on DNA and is involved in the regulation of various important cellular processes such as differentiation, proliferation, and tumor transformation and also in the regulation of the molecular events involved in the recovery of cell from DNA damage. Plays a fundamental role in organizing chromatin on a global scale; isoform e autoregulates Parp transcription by influencing the chromatin structure of its heterochromatic environment.1 Publication

Miscellaneous

The ADP-D-ribosyl group of NAD+ is transferred to an acceptor carboxyl group on a histone or the enzyme itself, and further ADP-ribosyl groups are transferred to the 2'-position of the terminal adenosine moiety, building up a polymer with an average chain length of 20-30 units.

Catalytic activityi

NAD+ + (ADP-D-ribosyl)(n)-acceptor = nicotinamide + (ADP-D-ribosyl)(n+1)-acceptor.PROSITE-ProRule annotation

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
DNA bindingi1 – 367Add BLAST367
Zinc fingeri7 – 89PARP-type 1PROSITE-ProRule annotationAdd BLAST83
Zinc fingeri111 – 200PARP-type 2PROSITE-ProRule annotationAdd BLAST90

GO - Molecular functioni

  • DNA binding Source: UniProtKB-KW
  • DNA ligase (ATP) activity Source: GO_Central
  • histone binding Source: FlyBase
  • NAD+ ADP-ribosyltransferase activity Source: FlyBase
  • NAD binding Source: InterPro
  • zinc ion binding Source: InterPro

GO - Biological processi

  • Cajal body organization Source: FlyBase
  • chromatin organization Source: UniProtKB-KW
  • chromosome organization Source: FlyBase
  • DNA ligation involved in DNA repair Source: GO_Central
  • dorsal/ventral axis specification, ovarian follicular epithelium Source: FlyBase
  • heat shock-mediated polytene chromosome puffing Source: FlyBase
  • innate immune response Source: FlyBase
  • lagging strand elongation Source: GO_Central
  • maintenance of protein location in nucleus Source: FlyBase
  • metamorphosis Source: FlyBase
  • nucleolus organization Source: FlyBase
  • polytene chromosome puffing Source: FlyBase
  • positive regulation of antibacterial peptide biosynthetic process Source: FlyBase
  • positive regulation of protein localization to nucleus Source: CACAO
  • protein ADP-ribosylation Source: FlyBase
  • regulation of RNA splicing Source: FlyBase
  • regulation of transcription, DNA-templated Source: FlyBase
  • rRNA transcription Source: FlyBase

Keywordsi

Molecular functionChromatin regulator, DNA-binding, Glycosyltransferase, Transferase
LigandMetal-binding, NAD, Zinc

Enzyme and pathway databases

BRENDAi2.4.2.30 1994
ReactomeiR-DME-110362 POLB-Dependent Long Patch Base Excision Repair
R-DME-2173795 Downregulation of SMAD2/3:SMAD4 transcriptional activity
R-DME-3108214 SUMOylation of DNA damage response and repair proteins
R-DME-5696394 DNA Damage Recognition in GG-NER
R-DME-5696395 Formation of Incision Complex in GG-NER
R-DME-5696400 Dual Incision in GG-NER

Names & Taxonomyi

Protein namesi
Recommended name:
Poly [ADP-ribose] polymerase (EC:2.4.2.30)
Short name:
PARP
Alternative name(s):
NAD(+) ADP-ribosyltransferase
Short name:
ADPRT
Poly[ADP-ribose] synthase
Gene namesi
Name:Parp
ORF Names:CG40411
OrganismiDrosophila melanogaster (Fruit fly)
Taxonomic identifieri7227 [NCBI]
Taxonomic lineageiEukaryotaMetazoaEcdysozoaArthropodaHexapodaInsectaPterygotaNeopteraHolometabolaDipteraBrachyceraMuscomorphaEphydroideaDrosophilidaeDrosophilaSophophora
Proteomesi
  • UP000000803 Componenti: Chromosome 3R

Organism-specific databases

FlyBaseiFBgn0010247 Parp

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002113251 – 994Poly [ADP-ribose] polymeraseAdd BLAST994

Keywords - PTMi

ADP-ribosylation

Proteomic databases

PaxDbiP35875
PRIDEiP35875

Expressioni

Tissue specificityi

Expressed in adult female oocytes, anal plates of stage 12 embryos and in cells around the central nervous system in later embryos.1 Publication

Developmental stagei

Expressed both maternally and zygotically in embryos, pupae and adults. Expression is highest in embryos.2 Publications

Gene expression databases

BgeeiFBgn0010247

Interactioni

Binary interactionsi

WithEntry#Exp.IntActNotes
IswiQ243684EBI-266172,EBI-367628

GO - Molecular functioni

  • histone binding Source: FlyBase

Protein-protein interaction databases

BioGridi78234, 37 interactors
DIPiDIP-23413N
IntActiP35875, 2 interactors
STRINGi7227.FBpp0112608

Structurei

3D structure databases

ProteinModelPortaliP35875
SMRiP35875
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini380 – 471BRCTPROSITE-ProRule annotationAdd BLAST92
Domaini644 – 761PARP alpha-helicalPROSITE-ProRule annotationAdd BLAST118
Domaini770 – 994PARP catalyticPROSITE-ProRule annotationAdd BLAST225

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni368 – 507Automodification domainAdd BLAST140

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi208 – 210Nuclear localization signal3
Motifi223 – 228Nuclear localization signal6

Zinc finger

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri7 – 89PARP-type 1PROSITE-ProRule annotationAdd BLAST83
Zinc fingeri111 – 200PARP-type 2PROSITE-ProRule annotationAdd BLAST90

Keywords - Domaini

Repeat, Zinc-finger

Phylogenomic databases

eggNOGiKOG1037 Eukaryota
ENOG410XP18 LUCA
GeneTreeiENSGT00390000017341
InParanoidiP35875
KOiK10798
OMAiWNHASCI
OrthoDBiEOG091G13H1
PhylomeDBiP35875

Family and domain databases

CDDicd00027 BRCT, 1 hit
Gene3Di1.20.142.10, 1 hit
2.20.140.10, 1 hit
2.20.25.630, 1 hit
3.30.1740.10, 2 hits
3.40.50.10190, 1 hit
InterProiView protein in InterPro
IPR001357 BRCT_dom
IPR036420 BRCT_dom_sf
IPR012982 PADR1
IPR038650 PADR1_dom_sf
IPR008288 PARP
IPR012317 Poly(ADP-ribose)pol_cat_dom
IPR004102 Poly(ADP-ribose)pol_reg_dom
IPR036616 Poly(ADP-ribose)pol_reg_dom_sf
IPR036930 WGR_dom_sf
IPR008893 WGR_domain
IPR001510 Znf_PARP
IPR036957 Znf_PARP_sf
PfamiView protein in Pfam
PF00533 BRCT, 1 hit
PF08063 PADR1, 1 hit
PF00644 PARP, 1 hit
PF02877 PARP_reg, 1 hit
PF05406 WGR, 1 hit
PF00645 zf-PARP, 2 hits
PIRSFiPIRSF000489 NAD_ADPRT, 1 hit
SMARTiView protein in SMART
SM00292 BRCT, 1 hit
SM01335 PADR1, 1 hit
SM00773 WGR, 1 hit
SM01336 zf-PARP, 2 hits
SUPFAMiSSF142921 SSF142921, 1 hit
SSF47587 SSF47587, 1 hit
SSF52113 SSF52113, 1 hit
PROSITEiView protein in PROSITE
PS50172 BRCT, 1 hit
PS51060 PARP_ALPHA_HD, 1 hit
PS51059 PARP_CATALYTIC, 1 hit
PS00347 PARP_ZN_FINGER_1, 1 hit
PS50064 PARP_ZN_FINGER_2, 2 hits

Sequences (3)i

Sequence statusi: Complete.

This entry describes 3 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform II (identifier: P35875-1) [UniParc]FASTAAdd to basket
Also known as: B, Long

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MDIELPYLAE YARTGRATCK GCKSTISKDT LRIAVMVQSA FHDAKVPNWF
60 70 80 90 100
HKTCFFKNQR PSSVGDIQNI GNLRFADQKE LTDLVENIQE VISAQLGKKR
110 120 130 140 150
SKAFNLALKD FGIEYAKSSR STCRGCEQKI NKDLVRLRKT VYDTEVGMKY
160 170 180 190 200
GGQPLWHHLE CFAQLRSELG WFASGEDMPG FQSLADDDQA KVKNAIPPIK
210 220 230 240 250
SEELPDTKRA KMELSDTNEE GEKKQRLKDQ NDAYFRFRDD IKNKMKKKDI
260 270 280 290 300
DILLKFNNQQ PVTGDTEKLF DQTADLLTFG AIESCSECNS CQFIVNKSGY
310 320 330 340 350
ICNGNHSEWT KCNKLLKEPT RSACIVPKEL KALYNFLNTV KEIPSTRIFN
360 370 380 390 400
NFPPNKSTFS RSLLKTNKNN DVLVRPTIPR ISPPLYNLKF SIIGLKNQHK
410 420 430 440 450
ELRKRIENLG GKFEVKISEN TIAIISTELE IQKKSTRMKF AEELGIHIVP
460 470 480 490 500
IEFLDFVEAD TEGAIKYINS TCICSWGTDP KSRIPKETTK SLNSNSIYTK
510 520 530 540 550
SMPVSRTFKV KDGLAVDPDS GLEDIAHVYV DSNNKYSVVL GLTDIQRNKN
560 570 580 590 600
SYYKVQLLKA DKKEKYWIFR SWGRIGTNIG NSKLEEFDTS ESAKRNFKEI
610 620 630 640 650
YADKTGNEYE QRDNFVKRTG RMYPIEIQYD DDQKLVKHES HFFTSKLEIS
660 670 680 690 700
VQNLIKLIFD IDSMNKTLME FHIDMDKMPL GKLSAHQIQS AYRVVKEIYN
710 720 730 740 750
VLECGSNTAK LIDATNRFYT LIPHNFGVQL PTLIETHQQI EDLRQMLDSL
760 770 780 790 800
AEIEVAYSII KSEDVSDACN PLDNHYAQIK TQLVALDKNS EEFSILSQYV
810 820 830 840 850
KNTHASTHKS YDLKIVDVFK VSRQGEARRF KPFKKLHNRK LLWHGSRLTN
860 870 880 890 900
FVGILSHGLR IAPPEAPPTG YMFGKGIYFA DMVSKSANYC CTSQQNSTGL
910 920 930 940 950
MLLSEVALGD MMECTSAKYI NKLSNNKHSC FGRGRTMPDP TKSYIRSDGV
960 970 980 990
EIPYGETITD EHLKSSLLYN EYIVYDVAQV NIQYLFRMEF KYSY
Length:994
Mass (Da):113,792
Last modified:June 1, 1994 - v1
Checksum:iACA85A270DD29E08
GO
Isoform I (identifier: P35875-2) [UniParc]FASTAAdd to basket
Also known as: Short

The sequence of this isoform differs from the canonical sequence as follows:
     376-565: Missing.

Show »
Length:804
Mass (Da):92,303
Checksum:i52719FF12193871F
GO
Isoform E (identifier: P35875-3) [UniParc]FASTAAdd to basket
Also known as: Embryonic

The sequence of this isoform differs from the canonical sequence as follows:
     607-613: NEYEQRD → MHFSEIH
     614-994: Missing.

Show »
Length:613
Mass (Da):69,956
Checksum:iB82605E1E3804868
GO

Sequence cautioni

The sequence AAM50807 differs from that shown. Reason: Erroneous initiation.Curated

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_004536376 – 565Missing in isoform I. CuratedAdd BLAST190
Alternative sequenceiVSP_013203607 – 613NEYEQRD → MHFSEIH in isoform E. 1 Publication7
Alternative sequenceiVSP_013204614 – 994Missing in isoform E. 1 PublicationAdd BLAST381

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D13806 mRNA Translation: BAA02964.1
AF051548
, AF051544, AF051545, AF051546, AF051547 Genomic DNA Translation: AAC24518.1
AF533701 mRNA Translation: AAM93435.1
AE014297 Genomic DNA Translation: EDP28045.1
BT015238 mRNA Translation: AAT94467.1
AY118947 mRNA Translation: AAM50807.1 Different initiation.
PIRiA47474
RefSeqiNP_001104452.1, NM_001110982.3 [P35875-1]
UniGeneiDm.7847

Genome annotation databases

EnsemblMetazoaiFBtr0113885; FBpp0112608; FBgn0010247 [P35875-1]
GeneIDi3355109
KEGGidme:Dmel_CG40411

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Entry informationi

Entry nameiPARP_DROME
AccessioniPrimary (citable) accession number: P35875
Secondary accession number(s): A8Y590
, Q7PLT6, Q8MSB5, Q8MU87, Q9W5Q5, Q9W5S1
Entry historyiIntegrated into UniProtKB/Swiss-Prot: June 1, 1994
Last sequence update: June 1, 1994
Last modified: May 23, 2018
This is version 196 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programDrosophila annotation project

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

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