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Protein

50S ribosomal protein L33

Gene

rpmG

Organism
Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Found on the solvent side of the large subunit.
Contacts the E site tRNA.

GO - Molecular functioni

  1. rRNA binding Source: UniProtKB-KW
  2. structural constituent of ribosome Source: InterPro
  3. tRNA binding Source: UniProtKB-KW

GO - Biological processi

  1. translation Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Ribonucleoprotein, Ribosomal protein

Keywords - Ligandi

RNA-binding, rRNA-binding, tRNA-binding

Enzyme and pathway databases

BioCyciTTHE300852:GH8R-260-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
50S ribosomal protein L33
Gene namesi
Name:rpmG
Synonyms:rpl33
Ordered Locus Names:TTHA0250
OrganismiThermus thermophilus (strain HB8 / ATCC 27634 / DSM 579)
Taxonomic identifieri300852 [NCBI]
Taxonomic lineageiBacteriaDeinococcus-ThermusDeinococciThermalesThermaceaeThermus
ProteomesiUP000000532 Componenti: Chromosome

Subcellular locationi

GO - Cellular componenti

  1. ribosome Source: UniProtKB-KW
Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methioninei1 – 11Removed1 Publication
Chaini2 – 545350S ribosomal protein L33PRO_0000170257Add
BLAST

Interactioni

Subunit structurei

Part of the 50S ribosomal subunit.

Protein-protein interaction databases

STRINGi300852.TTHA0250.

Structurei

Secondary structure

1
54
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi4 – 1310Combined sources
Turni14 – 163Combined sources
Beta strandi19 – 268Combined sources
Turni27 – 293Combined sources
Beta strandi35 – 406Combined sources
Turni41 – 444Combined sources
Beta strandi45 – 528Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1VVJX-ray3.44R6/Y61-54[»]
1VY4X-ray2.60B6/D61-54[»]
1VY5X-ray2.55B6/D61-54[»]
1VY6X-ray2.90B6/D61-54[»]
1VY7X-ray2.80B6/D61-54[»]
4L47X-ray3.22R6/Y61-54[»]
4L71X-ray3.90R6/Y61-54[»]
4LELX-ray3.90R6/Y61-54[»]
4LFZX-ray3.92R6/Y61-54[»]
4LNTX-ray2.94R6/Y61-54[»]
4LSKX-ray3.48R6/Y61-54[»]
4LT8X-ray3.14R6/Y61-54[»]
4P6FX-ray3.60R6/Y61-54[»]
4P70X-ray3.68R6/Y61-54[»]
4V4PX-ray5.50625-39[»]
4V4XX-ray5.00B51-54[»]
4V4YX-ray5.50B51-54[»]
4V4ZX-ray4.51B51-54[»]
4V51X-ray2.80B6/D62-54[»]
4V5AX-ray3.50B6/D62-54[»]
4V5CX-ray3.30B6/D61-54[»]
4V5DX-ray3.50B6/D61-54[»]
4V5EX-ray3.45B6/D61-54[»]
4V5FX-ray3.60B6/D61-54[»]
4V5GX-ray3.60B6/D61-54[»]
4V5JX-ray3.10B6/D61-54[»]
4V5KX-ray3.20B6/D61-54[»]
4V5LX-ray3.10B61-54[»]
4V5Melectron microscopy7.80B61-54[»]
4V5Nelectron microscopy7.60B61-54[»]
4V5PX-ray3.10B6/D61-54[»]
4V5QX-ray3.10B6/D61-54[»]
4V5RX-ray3.10B6/D61-54[»]
4V5SX-ray3.10B6/D61-54[»]
4V68electron microscopy6.40B69-53[»]
4V6AX-ray3.10B6/D61-54[»]
4V6FX-ray3.10A6/D61-54[»]
4V6GX-ray3.50B6/D61-54[»]
4V7JX-ray3.30A6/B61-54[»]
4V7KX-ray3.60A6/B61-54[»]
4V7LX-ray3.00B6/D61-54[»]
4V7MX-ray3.45B6/D61-54[»]
4V7WX-ray3.00B6/D61-54[»]
4V7XX-ray3.00B6/D61-54[»]
4V7YX-ray3.00B6/D61-54[»]
4V7ZX-ray3.10B6/D61-54[»]
4V87X-ray3.10A6/D69-53[»]
4V8AX-ray3.20A6/B61-54[»]
4V8BX-ray3.00B6/D61-54[»]
4V8CX-ray3.30A6/B61-54[»]
4V8DX-ray3.00B6/D61-54[»]
4V8EX-ray3.30A6/C61-54[»]
4V8FX-ray3.30A6/D61-54[»]
4V8GX-ray3.00B6/D61-54[»]
4V8HX-ray3.10B6/D61-54[»]
4V8IX-ray2.70B6/D61-54[»]
4V8JX-ray3.90B6/D61-54[»]
4V8NX-ray3.10B6/D61-54[»]
4V8OX-ray3.80B61-54[»]
4V8QX-ray3.10A61-54[»]
4V8UX-ray3.70B6/D61-54[»]
4V8XX-ray3.35B6/D61-54[»]
4V90X-ray2.95B62-54[»]
4V95X-ray3.20B6/D61-54[»]
4V97X-ray3.52B6/D61-54[»]
4V9AX-ray3.30B6/D61-54[»]
4V9BX-ray3.10B6/D61-54[»]
4V9HX-ray2.86B61-54[»]
4V9IX-ray3.30B6/D69-52[»]
4V9RX-ray3.00B6/D61-54[»]
4V9SX-ray3.10B6/D61-54[»]
4W2EX-ray2.9061-54[»]
4W2FX-ray2.40B6/D61-54[»]
4W2GX-ray2.55B6/D61-54[»]
4W2HX-ray2.70B6/D61-54[»]
4W2IX-ray2.70B6/D61-54[»]
ProteinModelPortaliP35871.
SMRiP35871. Positions 6-54.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP35871.

Family & Domainsi

Sequence similaritiesi

Belongs to the ribosomal protein L33P family.Curated

Phylogenomic databases

eggNOGiCOG0267.
HOGENOMiHOG000004838.
KOiK02913.
OMAiLELAKFC.
OrthoDBiEOG60KNBM.
PhylomeDBiP35871.

Family and domain databases

HAMAPiMF_00294. Ribosomal_L33.
InterProiIPR001705. Ribosomal_L33.
IPR018264. Ribosomal_L33_CS.
IPR011332. Ribosomal_zn-bd.
[Graphical view]
PfamiPF00471. Ribosomal_L33. 1 hit.
[Graphical view]
SUPFAMiSSF57829. SSF57829. 1 hit.
TIGRFAMsiTIGR01023. rpmG_bact. 1 hit.
PROSITEiPS00582. RIBOSOMAL_L33. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P35871-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MASEVRIKLL LECTECKRRN YATEKNKRNT PNKLELRKYC PWCRKHTVHR

EVKI
Length:54
Mass (Da):6,616
Last modified:January 22, 2007 - v3
Checksum:i13DC56C3247C60CD
GO

Sequence cautioni

The sequence L10348 differs from that shown. Reason: Frameshift at position 45. Curated

Mass spectrometryi

Molecular mass is 6486 Da from positions 2 - 54. Determined by MALDI. 1 Publication

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X61957 Genomic DNA. No translation available.
L10348 Genomic DNA. No translation available.
AP008226 Genomic DNA. Translation: BAD70073.1.
RefSeqiYP_143516.1. NC_006461.1.

Genome annotation databases

EnsemblBacteriaiBAD70073; BAD70073; BAD70073.
GeneIDi3167958.
KEGGittj:TTHA0250.
PATRICi23955447. VBITheThe93045_0250.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X61957 Genomic DNA. No translation available.
L10348 Genomic DNA. No translation available.
AP008226 Genomic DNA. Translation: BAD70073.1.
RefSeqiYP_143516.1. NC_006461.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1VVJX-ray3.44R6/Y61-54[»]
1VY4X-ray2.60B6/D61-54[»]
1VY5X-ray2.55B6/D61-54[»]
1VY6X-ray2.90B6/D61-54[»]
1VY7X-ray2.80B6/D61-54[»]
4L47X-ray3.22R6/Y61-54[»]
4L71X-ray3.90R6/Y61-54[»]
4LELX-ray3.90R6/Y61-54[»]
4LFZX-ray3.92R6/Y61-54[»]
4LNTX-ray2.94R6/Y61-54[»]
4LSKX-ray3.48R6/Y61-54[»]
4LT8X-ray3.14R6/Y61-54[»]
4P6FX-ray3.60R6/Y61-54[»]
4P70X-ray3.68R6/Y61-54[»]
4V4PX-ray5.50625-39[»]
4V4XX-ray5.00B51-54[»]
4V4YX-ray5.50B51-54[»]
4V4ZX-ray4.51B51-54[»]
4V51X-ray2.80B6/D62-54[»]
4V5AX-ray3.50B6/D62-54[»]
4V5CX-ray3.30B6/D61-54[»]
4V5DX-ray3.50B6/D61-54[»]
4V5EX-ray3.45B6/D61-54[»]
4V5FX-ray3.60B6/D61-54[»]
4V5GX-ray3.60B6/D61-54[»]
4V5JX-ray3.10B6/D61-54[»]
4V5KX-ray3.20B6/D61-54[»]
4V5LX-ray3.10B61-54[»]
4V5Melectron microscopy7.80B61-54[»]
4V5Nelectron microscopy7.60B61-54[»]
4V5PX-ray3.10B6/D61-54[»]
4V5QX-ray3.10B6/D61-54[»]
4V5RX-ray3.10B6/D61-54[»]
4V5SX-ray3.10B6/D61-54[»]
4V68electron microscopy6.40B69-53[»]
4V6AX-ray3.10B6/D61-54[»]
4V6FX-ray3.10A6/D61-54[»]
4V6GX-ray3.50B6/D61-54[»]
4V7JX-ray3.30A6/B61-54[»]
4V7KX-ray3.60A6/B61-54[»]
4V7LX-ray3.00B6/D61-54[»]
4V7MX-ray3.45B6/D61-54[»]
4V7WX-ray3.00B6/D61-54[»]
4V7XX-ray3.00B6/D61-54[»]
4V7YX-ray3.00B6/D61-54[»]
4V7ZX-ray3.10B6/D61-54[»]
4V87X-ray3.10A6/D69-53[»]
4V8AX-ray3.20A6/B61-54[»]
4V8BX-ray3.00B6/D61-54[»]
4V8CX-ray3.30A6/B61-54[»]
4V8DX-ray3.00B6/D61-54[»]
4V8EX-ray3.30A6/C61-54[»]
4V8FX-ray3.30A6/D61-54[»]
4V8GX-ray3.00B6/D61-54[»]
4V8HX-ray3.10B6/D61-54[»]
4V8IX-ray2.70B6/D61-54[»]
4V8JX-ray3.90B6/D61-54[»]
4V8NX-ray3.10B6/D61-54[»]
4V8OX-ray3.80B61-54[»]
4V8QX-ray3.10A61-54[»]
4V8UX-ray3.70B6/D61-54[»]
4V8XX-ray3.35B6/D61-54[»]
4V90X-ray2.95B62-54[»]
4V95X-ray3.20B6/D61-54[»]
4V97X-ray3.52B6/D61-54[»]
4V9AX-ray3.30B6/D61-54[»]
4V9BX-ray3.10B6/D61-54[»]
4V9HX-ray2.86B61-54[»]
4V9IX-ray3.30B6/D69-52[»]
4V9RX-ray3.00B6/D61-54[»]
4V9SX-ray3.10B6/D61-54[»]
4W2EX-ray2.9061-54[»]
4W2FX-ray2.40B6/D61-54[»]
4W2GX-ray2.55B6/D61-54[»]
4W2HX-ray2.70B6/D61-54[»]
4W2IX-ray2.70B6/D61-54[»]
ProteinModelPortaliP35871.
SMRiP35871. Positions 6-54.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi300852.TTHA0250.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiBAD70073; BAD70073; BAD70073.
GeneIDi3167958.
KEGGittj:TTHA0250.
PATRICi23955447. VBITheThe93045_0250.

Phylogenomic databases

eggNOGiCOG0267.
HOGENOMiHOG000004838.
KOiK02913.
OMAiLELAKFC.
OrthoDBiEOG60KNBM.
PhylomeDBiP35871.

Enzyme and pathway databases

BioCyciTTHE300852:GH8R-260-MONOMER.

Miscellaneous databases

EvolutionaryTraceiP35871.

Family and domain databases

HAMAPiMF_00294. Ribosomal_L33.
InterProiIPR001705. Ribosomal_L33.
IPR018264. Ribosomal_L33_CS.
IPR011332. Ribosomal_zn-bd.
[Graphical view]
PfamiPF00471. Ribosomal_L33. 1 hit.
[Graphical view]
SUPFAMiSSF57829. SSF57829. 1 hit.
TIGRFAMsiTIGR01023. rpmG_bact. 1 hit.
PROSITEiPS00582. RIBOSOMAL_L33. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Molecular cloning, nucleotide sequence and expression of the tufB gene encoding elongation factor Tu from Thermus thermophilus HB8."
    Satoh M., Tanaka T., Kushiro A., Hakoshima T., Tomita K.
    FEBS Lett. 288:98-100(1990) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  2. "Identification of the gene encoding transcription factor NusG of Thermus thermophilus."
    Heinrich T., Schroeder W., Erdmann V.A., Hartmann R.K.
    J. Bacteriol. 174:7859-7863(1991) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: HB8 / ATCC 27634 / DSM 579.
  3. "Complete genome sequence of Thermus thermophilus HB8."
    Masui R., Kurokawa K., Nakagawa N., Tokunaga F., Koyama Y., Shibata T., Oshima T., Yokoyama S., Yasunaga T., Kuramitsu S.
    Submitted (OCT-2004) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: HB8 / ATCC 27634 / DSM 579.
  4. "Identification of the 50S ribosomal proteins from the eubacterium Thermus thermophilus."
    Katsani K.R., Tsiboli P., Anagnostopoulos K., Urlaub H., Choli-Papadopoulou T.
    Biol. Chem. 381:1079-1087(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: PROTEIN SEQUENCE OF 2-23.
  5. "Identification of genes encoding ribosomal protein L33 from Bacillus licheniformis, Thermus thermophilus and Thermotoga maritima."
    Sharp P.M.
    Gene 139:135-136(1993) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION.
  6. "Extending ribosomal protein identifications to unsequenced bacterial strains using matrix-assisted laser desorption/ionization mass spectrometry."
    Suh M.-J., Hamburg D.M., Gregory S.T., Dahlberg A.E., Limbach P.A.
    Proteomics 5:4818-4831(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: MASS SPECTROMETRY.
  7. Cited for: STRUCTURE OF THE RIBOSOME.

Entry informationi

Entry nameiRL33_THET8
AccessioniPrimary (citable) accession number: P35871
Secondary accession number(s): Q5SLP3
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 31, 1994
Last sequence update: January 22, 2007
Last modified: March 3, 2015
This is version 113 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. Ribosomal proteins
    Ribosomal proteins families and list of entries
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.