P35868 (SYR_CORGL) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Arginine--tRNA ligase
EC=6.1.1.19

Alternative name(s):
Arginyl-tRNA synthetase
Short name=ArgRS

Gene names

Name:	argS
Ordered Locus Names:	Cgl1179, cg1333

Organism

Corynebacterium glutamicum (strain ATCC 13032 / DSM 20300 / JCM 1318 / LMG 3730 / NCIMB 10025) [Reference proteome] [HAMAP]

Taxonomic identifier

196627 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Actinobacteria › Actinobacteria › Actinobacteridae › Actinomycetales › Corynebacterineae › Corynebacteriaceae › Corynebacterium › Corynebacterium glutamicum

Protein attributes

Sequence length	550 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Catalytic activity	ATP + L-arginine + tRNA(Arg) = AMP + diphosphate + L-arginyl-tRNA(Arg). HAMAP-Rule MF_00123
Subunit structure	Monomer By similarity.
Subcellular location	Cytoplasm By similarity.
Induction	Up-regulated by arginine and repressed by lysine. Ref.2
Sequence similarities	Belongs to the class-I aminoacyl-tRNA synthetase family.
Sequence caution	The sequence CAA38537.1 differs from that shown. Reason: Erroneous initiation.

Ontologies

Keywords
Biological process	Protein biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Aminoacyl-tRNA synthetase Ligase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	arginyl-tRNA aminoacylation Inferred from electronic annotation. Source: HAMAP
Cellular_component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	ATP binding Inferred from electronic annotation. Source: HAMAP arginine-tRNA ligase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 550	550	Arginine--tRNA ligase HAMAP-Rule MF_00123		PRO_0000151554
Regions
	Motif	130 – 140	11	"HIGH" region HAMAP-Rule MF_00123
Experimental info
	Sequence conflict	355	1	G → D in CAA79710. Ref.2
	Sequence conflict	412	1	I → M in CAA79710. Ref.2
	Sequence conflict	513	1	V → A in CAA79710. Ref.2
	Sequence conflict	540	1	H → R in CAA79710. Ref.2

Sequences

	Sequence		Length	Mass (Da)
	P35868 [UniParc]. Last modified February 1, 1996. Version 2. Checksum: 3AF724BDEE8DC4C1	FASTA	550	59,723
	10 20 30 40 50 60 MTPADLATLI KETAVEVLTS RELDTSVLPE QVVVERPRNP EHGDYATNIA LQVAKKVGQN 70 80 90 100 110 120 PRDLATWLAE ALAADDAIDS AEIAGPGFLN IRLAAAAQGE IVAKILAQGE TFGNSDHLSH 130 140 150 160 170 180 LDVNLEFVSA NPTGPIHLGG TRWAAVGDSL GRVLEASGAK VTREYYFNDH GRQIDRFALS 190 200 210 220 230 240 LLAAAKGEPT PEDGYGGEYI KEIAEAIVEK HPEALALEPA ATQELFRAEG VEMMFEHIKS 250 260 270 280 290 300 SLHEFGTDFD VYYHENSLFE SGAVDKAVQV LKDNGNLYEN EGAWWLRSTE FGDDKDRVVI 310 320 330 340 350 360 KSDGDAAYIA GDIAYVADKF SRGHNLNIYM LGADHHGYIA RLKAAAAALG YKPEGVEVLI 370 380 390 400 410 420 GQMVNLLRDG KAVRMSKRAG TVVTLDDLVE AIGIDAARYS LIRSSVDSSL DIDLGLWESQ 430 440 450 460 470 480 SSDNPVYYVQ YGHARLCSIA RKAETLGVTE EGADLSLLTH DREGDLIRTL GEFPAVVKAA 490 500 510 520 530 540 ADLREPHRIA RYAEELAGTF HRFYDSCHIL PKVDEDTAPI HTARLALAAA TRQTLANALH 550 LVGVSAPEKM

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Nucleotide sequence and organization of the upstream region of the Corynebacterium glutamicum lysA gene." Marcel T., Archer J.A.C., Mengin-Lecreulx D., Sinskey A.J. Mol. Microbiol. 4:1819-1830(1990) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 13059 / LMG 3658 / NCIB 10332 / AS019 / 613.
[2]	"A gene encoding arginyl-tRNA synthetase is located in the upstream region of the lysA gene in Brevibacterium lactofermentum: regulation of argS-lysA cluster expression by arginine." Oguiza J.A., Malumbres M., Eriani G., Pisabarro A., Mateos L.M., Martin F., Martin J.F. J. Bacteriol. 175:7356-7362(1993) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], INDUCTION. Strain: ATCC 13869 / DSMZ 1412 / NCIMB 9567.
[3]	"The Corynebacterium glutamicum genome: features and impacts on biotechnological processes." Ikeda M., Nakagawa S. Appl. Microbiol. Biotechnol. 62:99-109(2003) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 13032 / DSM 20300 / JCM 1318 / LMG 3730 / NCIMB 10025.
[4]	"The complete Corynebacterium glutamicum ATCC 13032 genome sequence and its impact on the production of L-aspartate-derived amino acids and vitamins." Kalinowski J., Bathe B., Bartels D., Bischoff N., Bott M., Burkovski A., Dusch N., Eggeling L., Eikmanns B.J., Gaigalat L., Goesmann A., Hartmann M., Huthmacher K., Kraemer R., Linke B., McHardy A.C., Meyer F., Moeckel B. , Pfefferle W., Puehler A., Rey D.A., Rueckert C., Rupp O., Sahm H., Wendisch V.F., Wiegraebe I., Tauch A. J. Biotechnol. 104:5-25(2003) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 13032 / DSM 20300 / JCM 1318 / LMG 3730 / NCIMB 10025.
[5]	"Corynebacterium glutamicum arginyl-tRNA synthetase." Sharp P.M., Mitchell K.J. Mol. Microbiol. 8:200-200(1993) [PubMed] [Europe PMC] [Abstract] Cited for: IDENTIFICATION.

Cross-references

Sequence databases
EMBL GenBank DDBJ	X54740 Genomic DNA. Translation: CAA38537.1. Different initiation. Z21501 Genomic DNA. Translation: CAA79710.1. BA000036 Genomic DNA. Translation: BAB98572.1. BX927151 Genomic DNA. Translation: CAF19883.1.
PIR	A49936. S12227.
RefSeq	NP_600405.1. NC_003450.3. YP_225469.1. NC_006958.1.
3D structure databases
ProteinModelPortal	P35868.
ModBase	Search...
Protein-protein interaction databases
STRING	196627.cg1333.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	BAB98572; BAB98572; BAB98572. CAF19883; CAF19883; cg1333.
GeneID	1019162. 3343206.
KEGG	cgb:cg1333. cgl:NCgl1132.
PATRIC	21494404. VBICorGlu203724_1158.
Phylogenomic databases
eggNOG	COG0018.
KO	K01887.
OMA	HTSANPN.
ProtClustDB	PRK01611.
Enzyme and pathway databases
BioCyc	CGLU196627:GJDM-1165-MONOMER.
Family and domain databases
Gene3D	3.30.1360.70. 1 hit. 3.40.50.620. 1 hit.
HAMAP	MF_00123. Arg_tRNA_synth.
InterPro	IPR001412. aa-tRNA-synth_I_CS. IPR001278. Arg-tRNA-ligase_Ia. IPR015945. Arg-tRNA-synth_Ia_core. IPR005148. Arg-tRNA-synth_N. IPR008909. DALR_anticod-bd. IPR014729. Rossmann-like_a/b/a_fold. IPR009080. tRNAsynth_1a_anticodon-bd. [Graphical view]
PANTHER	PTHR11956. PTHR11956. 1 hit.
Pfam	PF03485. Arg_tRNA_synt_N. 1 hit. PF05746. DALR_1. 1 hit. PF00750. tRNA-synt_1d. 1 hit. [Graphical view]
PRINTS	PR01038. TRNASYNTHARG.
SMART	SM01016. Arg_tRNA_synt_N. 1 hit. SM00836. DALR_1. 1 hit. [Graphical view]
SUPFAM	SSF55190. Arg-tRNA-synth_Ic_N. 1 hit. SSF47323. tRNAsyn_1a_bind. 1 hit.
TIGRFAMs	TIGR00456. argS. 1 hit.
PROSITE	PS00178. AA_TRNA_LIGASE_I. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

SYR_CORGL

Accession

Primary (citable) accession number: P35868
Secondary accession number(s): P41253

Entry history

Integrated into UniProtKB/Swiss-Prot:	June 1, 1994
Last sequence update:	February 1, 1996
Last modified:	May 1, 2013
This is version 103 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program