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P35868 (SYR_CORGL) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 108. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Arginine--tRNA ligase

EC=6.1.1.19
Alternative name(s):
Arginyl-tRNA synthetase
Short name=ArgRS
Gene names
Name:argS
Ordered Locus Names:Cgl1179, cg1333
OrganismCorynebacterium glutamicum (strain ATCC 13032 / DSM 20300 / JCM 1318 / LMG 3730 / NCIMB 10025) [Reference proteome] [HAMAP]
Taxonomic identifier196627 [NCBI]
Taxonomic lineageBacteriaActinobacteriaActinobacteridaeActinomycetalesCorynebacterineaeCorynebacteriaceaeCorynebacterium

Protein attributes

Sequence length550 AA.
Sequence statusComplete.
Protein existenceEvidence at transcript level

General annotation (Comments)

Catalytic activity

ATP + L-arginine + tRNA(Arg) = AMP + diphosphate + L-arginyl-tRNA(Arg). HAMAP-Rule MF_00123

Subunit structure

Monomer By similarity. HAMAP-Rule MF_00123

Subcellular location

Cytoplasm By similarity HAMAP-Rule MF_00123.

Induction

Up-regulated by arginine and repressed by lysine. Ref.2

Sequence similarities

Belongs to the class-I aminoacyl-tRNA synthetase family.

Sequence caution

The sequence CAA38537.1 differs from that shown. Reason: Erroneous initiation.

Ontologies

Keywords
   Biological processProtein biosynthesis
   Cellular componentCytoplasm
   LigandATP-binding
Nucleotide-binding
   Molecular functionAminoacyl-tRNA synthetase
Ligase
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processarginyl-tRNA aminoacylation

Inferred from electronic annotation. Source: UniProtKB-HAMAP

   Cellular_componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionATP binding

Inferred from electronic annotation. Source: UniProtKB-HAMAP

arginine-tRNA ligase activity

Inferred from electronic annotation. Source: UniProtKB-HAMAP

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 550550Arginine--tRNA ligase HAMAP-Rule MF_00123
PRO_0000151554

Regions

Motif130 – 14011"HIGH" region HAMAP-Rule MF_00123

Experimental info

Sequence conflict3551G → D in CAA79710. Ref.2
Sequence conflict4121I → M in CAA79710. Ref.2
Sequence conflict5131V → A in CAA79710. Ref.2
Sequence conflict5401H → R in CAA79710. Ref.2

Sequences

Sequence LengthMass (Da)Tools
P35868 [UniParc].

Last modified February 1, 1996. Version 2.
Checksum: 3AF724BDEE8DC4C1

FASTA55059,723
        10         20         30         40         50         60 
MTPADLATLI KETAVEVLTS RELDTSVLPE QVVVERPRNP EHGDYATNIA LQVAKKVGQN 

        70         80         90        100        110        120 
PRDLATWLAE ALAADDAIDS AEIAGPGFLN IRLAAAAQGE IVAKILAQGE TFGNSDHLSH 

       130        140        150        160        170        180 
LDVNLEFVSA NPTGPIHLGG TRWAAVGDSL GRVLEASGAK VTREYYFNDH GRQIDRFALS 

       190        200        210        220        230        240 
LLAAAKGEPT PEDGYGGEYI KEIAEAIVEK HPEALALEPA ATQELFRAEG VEMMFEHIKS 

       250        260        270        280        290        300 
SLHEFGTDFD VYYHENSLFE SGAVDKAVQV LKDNGNLYEN EGAWWLRSTE FGDDKDRVVI 

       310        320        330        340        350        360 
KSDGDAAYIA GDIAYVADKF SRGHNLNIYM LGADHHGYIA RLKAAAAALG YKPEGVEVLI 

       370        380        390        400        410        420 
GQMVNLLRDG KAVRMSKRAG TVVTLDDLVE AIGIDAARYS LIRSSVDSSL DIDLGLWESQ 

       430        440        450        460        470        480 
SSDNPVYYVQ YGHARLCSIA RKAETLGVTE EGADLSLLTH DREGDLIRTL GEFPAVVKAA 

       490        500        510        520        530        540 
ADLREPHRIA RYAEELAGTF HRFYDSCHIL PKVDEDTAPI HTARLALAAA TRQTLANALH 

       550 
LVGVSAPEKM 

« Hide

References

« Hide 'large scale' references
[1]"Nucleotide sequence and organization of the upstream region of the Corynebacterium glutamicum lysA gene."
Marcel T., Archer J.A.C., Mengin-Lecreulx D., Sinskey A.J.
Mol. Microbiol. 4:1819-1830(1990) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: ATCC 13059 / LMG 3658 / NCIB 10332 / AS019 / 613.
[2]"A gene encoding arginyl-tRNA synthetase is located in the upstream region of the lysA gene in Brevibacterium lactofermentum: regulation of argS-lysA cluster expression by arginine."
Oguiza J.A., Malumbres M., Eriani G., Pisabarro A., Mateos L.M., Martin F., Martin J.F.
J. Bacteriol. 175:7356-7362(1993) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], INDUCTION.
Strain: ATCC 13869 / DSMZ 1412 / NCIMB 9567.
[3]"The Corynebacterium glutamicum genome: features and impacts on biotechnological processes."
Ikeda M., Nakagawa S.
Appl. Microbiol. Biotechnol. 62:99-109(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 13032 / DSM 20300 / JCM 1318 / LMG 3730 / NCIMB 10025.
[4]"The complete Corynebacterium glutamicum ATCC 13032 genome sequence and its impact on the production of L-aspartate-derived amino acids and vitamins."
Kalinowski J., Bathe B., Bartels D., Bischoff N., Bott M., Burkovski A., Dusch N., Eggeling L., Eikmanns B.J., Gaigalat L., Goesmann A., Hartmann M., Huthmacher K., Kraemer R., Linke B., McHardy A.C., Meyer F., Moeckel B. expand/collapse author list , Pfefferle W., Puehler A., Rey D.A., Rueckert C., Rupp O., Sahm H., Wendisch V.F., Wiegraebe I., Tauch A.
J. Biotechnol. 104:5-25(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 13032 / DSM 20300 / JCM 1318 / LMG 3730 / NCIMB 10025.
[5]"Corynebacterium glutamicum arginyl-tRNA synthetase."
Sharp P.M., Mitchell K.J.
Mol. Microbiol. 8:200-200(1993) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
X54740 Genomic DNA. Translation: CAA38537.1. Different initiation.
Z21501 Genomic DNA. Translation: CAA79710.1.
BA000036 Genomic DNA. Translation: BAB98572.1.
BX927151 Genomic DNA. Translation: CAF19883.1.
PIRA49936.
S12227.
RefSeqNP_600405.1. NC_003450.3.
YP_225469.1. NC_006958.1.

3D structure databases

ProteinModelPortalP35868.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING196627.cg1333.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaBAB98572; BAB98572; BAB98572.
CAF19883; CAF19883; cg1333.
GeneID1019162.
3343206.
KEGGcgb:cg1333.
cgl:NCgl1132.
PATRIC21494404. VBICorGlu203724_1158.

Phylogenomic databases

eggNOGCOG0018.
KOK01887.
OMANPNGPLH.
OrthoDBEOG6JB13C.
ProtClustDBPRK01611.

Family and domain databases

Gene3D1.10.730.10. 1 hit.
3.30.1360.70. 1 hit.
3.40.50.620. 1 hit.
HAMAPMF_00123. Arg_tRNA_synth.
InterProIPR001412. aa-tRNA-synth_I_CS.
IPR001278. Arg-tRNA-ligase.
IPR005148. Arg-tRNA-synth_N.
IPR008909. DALR_anticod-bd.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
[Graphical view]
PANTHERPTHR11956. PTHR11956. 1 hit.
PfamPF03485. Arg_tRNA_synt_N. 1 hit.
PF05746. DALR_1. 1 hit.
PF00750. tRNA-synt_1d. 1 hit.
[Graphical view]
PRINTSPR01038. TRNASYNTHARG.
SMARTSM01016. Arg_tRNA_synt_N. 1 hit.
SM00836. DALR_1. 1 hit.
[Graphical view]
SUPFAMSSF47323. SSF47323. 1 hit.
SSF55190. SSF55190. 1 hit.
TIGRFAMsTIGR00456. argS. 1 hit.
PROSITEPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameSYR_CORGL
AccessionPrimary (citable) accession number: P35868
Secondary accession number(s): P41253
Entry history
Integrated into UniProtKB/Swiss-Prot: June 1, 1994
Last sequence update: February 1, 1996
Last modified: April 16, 2014
This is version 108 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Aminoacyl-tRNA synthetases

List of aminoacyl-tRNA synthetase entries