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P35853 (PUR1_LACCA) Reviewed, UniProtKB/Swiss-Prot

Last modified June 11, 2014. Version 78. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
Amidophosphoribosyltransferase

Short name=ATase
EC=2.4.2.14
Alternative name(s):
Glutamine phosphoribosylpyrophosphate amidotransferase
Short name=GPATase
Gene names
Name:purF
OrganismLactobacillus casei
Taxonomic identifier1582 [NCBI]
Taxonomic lineageBacteriaFirmicutesBacilliLactobacillalesLactobacillaceaeLactobacillus

Protein attributes

Sequence length194 AA.
Sequence statusFragment.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceInferred from homology

General annotation (Comments)

Catalytic activity

5-phospho-beta-D-ribosylamine + diphosphate + L-glutamate = L-glutamine + 5-phospho-alpha-D-ribose 1-diphosphate + H2O.

Cofactor

Binds 1 magnesium ion per subunit Potential.

Binds 1 4Fe-4S cluster per subunit Potential.

Pathway

Purine metabolism; IMP biosynthesis via de novo pathway; N(1)-(5-phospho-D-ribosyl)glycinamide from 5-phospho-alpha-D-ribose 1-diphosphate: step 1/2.

Sequence similarities

In the C-terminal section; belongs to the purine/pyrimidine phosphoribosyltransferase family.

Contains 1 glutamine amidotransferase type-2 domain.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Propeptide1 – 1111 By similarity
PRO_0000029253
Chain12 – ›194›183Amidophosphoribosyltransferase
PRO_0000029254

Regions

Domain12 – ›194›183Glutamine amidotransferase type-2

Sites

Active site121For GATase activity By similarity

Experimental info

Non-terminal residue1941

Sequences

Sequence LengthMass (Da)Tools
P35853 [UniParc].

Last modified February 1, 1996. Version 2.
Checksum: 4A788CBC5365D5EC

FASTA19421,144
        10         20         30         40         50         60 
MPHEPKGLNE ECGVFGVWGN PNAASITHLG LHTLQHRGQE GAGIVGLTKD GMRRHYGLGL 

        70         80         90        100        110        120 
LSEVFTNTDQ LTPLIGRAAL GHVRYSTAGG RVLENIQPLL FRFSDEAIAL AHNGNLTNAI 

       130        140        150        160        170        180 
SLRRQLEDQG AIFQSTSDTE VLMHLIRRQV GQPWLTQLKT ALNEVHGGFA FVLLTEHGLY 

       190 
AAVDPHGFRP MVVG 

« Hide

References

[1]"Isolation and complete sequence of the purL gene encoding FGAM synthase II in Lactobacillus casei."
Gu Z.-M., Martindale D.W., Lee B.H.
Gene 119:123-126(1992) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]Erratum
Gu Z.-M., Martindale D.W., Lee B.H.
Gene 133:147-147(1993) [PubMed] [Europe PMC] [Abstract]

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
M85265 Genomic DNA. Translation: AAC36948.1.
PIRPC1136.

3D structure databases

ProteinModelPortalP35853.
SMRP35853. Positions 12-194.
ModBaseSearch...
MobiDBSearch...

Protein family/group databases

MEROPSC44.001.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Enzyme and pathway databases

UniPathwayUPA00074; UER00124.

Family and domain databases

Gene3D3.60.20.10. 1 hit.
InterProIPR017932. GATase_2_dom.
IPR029055. Ntn_hydrolases_N.
[Graphical view]
SUPFAMSSF56235. SSF56235. 1 hit.
PROSITEPS51278. GATASE_TYPE_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry namePUR1_LACCA
AccessionPrimary (citable) accession number: P35853
Entry history
Integrated into UniProtKB/Swiss-Prot: June 1, 1994
Last sequence update: February 1, 1996
Last modified: June 11, 2014
This is version 78 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways