SubmitCancel

Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.

P35853

- PUR1_LACCA

UniProt

P35853 - PUR1_LACCA

(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

Protein

Amidophosphoribosyltransferase

Gene
purF
Organism
Lactobacillus casei
Status
Reviewed - Annotation score: 3 out of 5 - Protein inferred from homologyi

Functioni

Catalytic activityi

5-phospho-beta-D-ribosylamine + diphosphate + L-glutamate = L-glutamine + 5-phospho-alpha-D-ribose 1-diphosphate + H2O.

Cofactori

Binds 1 magnesium ion per subunit Reviewed prediction.
Binds 1 4Fe-4S cluster per subunit Reviewed prediction.

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei12 – 121For GATase activity By similarity

GO - Molecular functioni

  1. 4 iron, 4 sulfur cluster binding Source: UniProtKB-KW
  2. amidophosphoribosyltransferase activity Source: UniProtKB-EC
  3. metal ion binding Source: UniProtKB-KW

GO - Biological processi

  1. 'de novo' IMP biosynthetic process Source: UniProtKB-UniPathway
  2. glutamine metabolic process Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Glycosyltransferase, Transferase

Keywords - Biological processi

Purine biosynthesis

Keywords - Ligandi

4Fe-4S, Iron, Iron-sulfur, Magnesium, Metal-binding

Enzyme and pathway databases

UniPathwayiUPA00074; UER00124.

Protein family/group databases

MEROPSiC44.001.

Names & Taxonomyi

Protein namesi
Recommended name:
Amidophosphoribosyltransferase (EC:2.4.2.14)
Short name:
ATase
Alternative name(s):
Glutamine phosphoribosylpyrophosphate amidotransferase
Short name:
GPATase
Gene namesi
Name:purF
OrganismiLactobacillus casei
Taxonomic identifieri1582 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliLactobacillalesLactobacillaceaeLactobacillus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Propeptidei1 – 1111 By similarityPRO_0000029253Add
BLAST
Chaini12 – ›194›183AmidophosphoribosyltransferasePRO_0000029254Add
BLAST

Structurei

3D structure databases

ProteinModelPortaliP35853.
SMRiP35853. Positions 12-194.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini12 – ›194›183Glutamine amidotransferase type-2Add
BLAST

Sequence similaritiesi

In the C-terminal section; belongs to the purine/pyrimidine phosphoribosyltransferase family.

Keywords - Domaini

Glutamine amidotransferase

Family and domain databases

Gene3Di3.60.20.10. 1 hit.
InterProiIPR017932. GATase_2_dom.
IPR029055. Ntn_hydrolases_N.
[Graphical view]
SUPFAMiSSF56235. SSF56235. 1 hit.
PROSITEiPS51278. GATASE_TYPE_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Fragment.

Sequence processingi: The displayed sequence is further processed into a mature form.

P35853-1 [UniParc]FASTAAdd to Basket

« Hide

MPHEPKGLNE ECGVFGVWGN PNAASITHLG LHTLQHRGQE GAGIVGLTKD    50
GMRRHYGLGL LSEVFTNTDQ LTPLIGRAAL GHVRYSTAGG RVLENIQPLL 100
FRFSDEAIAL AHNGNLTNAI SLRRQLEDQG AIFQSTSDTE VLMHLIRRQV 150
GQPWLTQLKT ALNEVHGGFA FVLLTEHGLY AAVDPHGFRP MVVG 194
Length:194
Mass (Da):21,144
Last modified:February 1, 1996 - v2
Checksum:i4A788CBC5365D5EC
GO

Non-terminal residue

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Non-terminal residuei194 – 1941

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
M85265 Genomic DNA. Translation: AAC36948.1.
PIRiPC1136.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
M85265 Genomic DNA. Translation: AAC36948.1 .
PIRi PC1136.

3D structure databases

ProteinModelPortali P35853.
SMRi P35853. Positions 12-194.
ModBasei Search...
MobiDBi Search...

Protein family/group databases

MEROPSi C44.001.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Enzyme and pathway databases

UniPathwayi UPA00074 ; UER00124 .

Family and domain databases

Gene3Di 3.60.20.10. 1 hit.
InterProi IPR017932. GATase_2_dom.
IPR029055. Ntn_hydrolases_N.
[Graphical view ]
SUPFAMi SSF56235. SSF56235. 1 hit.
PROSITEi PS51278. GATASE_TYPE_2. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Isolation and complete sequence of the purL gene encoding FGAM synthase II in Lactobacillus casei."
    Gu Z.-M., Martindale D.W., Lee B.H.
    Gene 119:123-126(1992) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].

Entry informationi

Entry nameiPUR1_LACCA
AccessioniPrimary (citable) accession number: P35853
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 1, 1994
Last sequence update: February 1, 1996
Last modified: June 11, 2014
This is version 78 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi