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P35853

- PUR1_LACCA

UniProt

P35853 - PUR1_LACCA

Protein

Amidophosphoribosyltransferase

Gene

purF

Organism
Lactobacillus casei
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
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    • History
      Entry version 79 (01 Oct 2014)
      Sequence version 2 (01 Feb 1996)
      Previous versions | rss
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    Functioni

    Catalytic activityi

    5-phospho-beta-D-ribosylamine + diphosphate + L-glutamate = L-glutamine + 5-phospho-alpha-D-ribose 1-diphosphate + H2O.

    Cofactori

    Binds 1 magnesium ion per subunit.Curated
    Binds 1 4Fe-4S cluster per subunit.Curated

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei12 – 121For GATase activityBy similarity

    GO - Molecular functioni

    1. 4 iron, 4 sulfur cluster binding Source: UniProtKB-KW
    2. amidophosphoribosyltransferase activity Source: UniProtKB-EC
    3. metal ion binding Source: UniProtKB-KW

    GO - Biological processi

    1. 'de novo' IMP biosynthetic process Source: UniProtKB-UniPathway
    2. glutamine metabolic process Source: UniProtKB-KW

    Keywords - Molecular functioni

    Glycosyltransferase, Transferase

    Keywords - Biological processi

    Purine biosynthesis

    Keywords - Ligandi

    4Fe-4S, Iron, Iron-sulfur, Magnesium, Metal-binding

    Enzyme and pathway databases

    UniPathwayiUPA00074; UER00124.

    Protein family/group databases

    MEROPSiC44.001.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Amidophosphoribosyltransferase (EC:2.4.2.14)
    Short name:
    ATase
    Alternative name(s):
    Glutamine phosphoribosylpyrophosphate amidotransferase
    Short name:
    GPATase
    Gene namesi
    Name:purF
    OrganismiLactobacillus casei
    Taxonomic identifieri1582 [NCBI]
    Taxonomic lineageiBacteriaFirmicutesBacilliLactobacillalesLactobacillaceaeLactobacillus

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Propeptidei1 – 1111By similarityPRO_0000029253Add
    BLAST
    Chaini12 – ›194›183AmidophosphoribosyltransferasePRO_0000029254Add
    BLAST

    Structurei

    3D structure databases

    ProteinModelPortaliP35853.
    SMRiP35853. Positions 12-194.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini12 – ›194›183Glutamine amidotransferase type-2PROSITE-ProRule annotationAdd
    BLAST

    Sequence similaritiesi

    In the C-terminal section; belongs to the purine/pyrimidine phosphoribosyltransferase family.Curated
    Contains 1 glutamine amidotransferase type-2 domain.PROSITE-ProRule annotation

    Keywords - Domaini

    Glutamine amidotransferase

    Family and domain databases

    Gene3Di3.60.20.10. 1 hit.
    InterProiIPR017932. GATase_2_dom.
    IPR029055. Ntn_hydrolases_N.
    [Graphical view]
    SUPFAMiSSF56235. SSF56235. 1 hit.
    PROSITEiPS51278. GATASE_TYPE_2. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Fragment.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    P35853-1 [UniParc]FASTAAdd to Basket

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    MPHEPKGLNE ECGVFGVWGN PNAASITHLG LHTLQHRGQE GAGIVGLTKD    50
    GMRRHYGLGL LSEVFTNTDQ LTPLIGRAAL GHVRYSTAGG RVLENIQPLL 100
    FRFSDEAIAL AHNGNLTNAI SLRRQLEDQG AIFQSTSDTE VLMHLIRRQV 150
    GQPWLTQLKT ALNEVHGGFA FVLLTEHGLY AAVDPHGFRP MVVG 194
    Length:194
    Mass (Da):21,144
    Last modified:February 1, 1996 - v2
    Checksum:i4A788CBC5365D5EC
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Non-terminal residuei194 – 1941

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M85265 Genomic DNA. Translation: AAC36948.1.
    PIRiPC1136.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M85265 Genomic DNA. Translation: AAC36948.1 .
    PIRi PC1136.

    3D structure databases

    ProteinModelPortali P35853.
    SMRi P35853. Positions 12-194.
    ModBasei Search...
    MobiDBi Search...

    Protein family/group databases

    MEROPSi C44.001.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Enzyme and pathway databases

    UniPathwayi UPA00074 ; UER00124 .

    Family and domain databases

    Gene3Di 3.60.20.10. 1 hit.
    InterProi IPR017932. GATase_2_dom.
    IPR029055. Ntn_hydrolases_N.
    [Graphical view ]
    SUPFAMi SSF56235. SSF56235. 1 hit.
    PROSITEi PS51278. GATASE_TYPE_2. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Isolation and complete sequence of the purL gene encoding FGAM synthase II in Lactobacillus casei."
      Gu Z.-M., Martindale D.W., Lee B.H.
      Gene 119:123-126(1992) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].

    Entry informationi

    Entry nameiPUR1_LACCA
    AccessioniPrimary (citable) accession number: P35853
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: June 1, 1994
    Last sequence update: February 1, 1996
    Last modified: October 1, 2014
    This is version 79 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Documents

    1. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3