P35852 (PURL_LACCA) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Phosphoribosylformylglycinamidine synthase 2
EC=6.3.5.3

Alternative name(s):
Phosphoribosylformylglycinamidine synthase II
Short name=FGAM synthase II

Gene names

Name:

purL

Organism

Lactobacillus casei

Taxonomic identifier

1582 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Lactobacillales › Lactobacillaceae › Lactobacillus

Protein attributes

Sequence length	740 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	ATP + N(2)-formyl-N(1)-(5-phospho-D-ribosyl)glycinamide + L-glutamine + H2O = ADP + phosphate + 2-(formamido)-N(1)-(5-phospho-D-ribosyl)acetamidine + L-glutamate. HAMAP MF_00420
Pathway	Purine metabolism; IMP biosynthesis via de novo pathway; 5-amino-1-(5-phospho-D-ribosyl)imidazole from N(2)-formyl-N(1)-(5-phospho-D-ribosyl)glycinamide: step 1/2. HAMAP MF_00420
Subunit structure	Heterodimer of two subunits, PurQ and PurL.
Subcellular location	Cytoplasm By similarity HAMAP MF_00420.
Sequence similarities	Belongs to the FGAMS family.

Ontologies

Keywords
Biological process	Purine biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Ligase
Gene Ontology (GO)
Biological process	'de novo' IMP biosynthetic process Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW phosphoribosylformylglycinamidine synthase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 740	740	Phosphoribosylformylglycinamidine synthase 2 HAMAP MF_00420		PRO_0000100460
Regions
	Nucleotide binding	112 – 123	12	ATP Potential

Sequences

	Sequence		Length	Mass (Da)
	P35852 [UniParc]. Last modified February 1, 1996. Version 2. Checksum: 03238AB3CD1C8FF5	FASTA	740	79,481
	10 20 30 40 50 60 MYVVHVEMSP EAIATQKPYL DLGLTEAEYD RFAELIGHQP NDTEIGLASG MWSEHCAYKY 70 80 90 100 110 120 SKPVLRQFWT KNERVLMGPG EGAGVIDIGE GKAVVFKAES HNHPSAVEPY EGAATGVGGI 130 140 150 160 170 180 IRDIFSIGAK PVAMLDSLAF GDIEQPHTQH LVDRIVAGIG GYGNAIGIPT VGGETNFDGS 190 200 210 220 230 240 YTRNPLVNAM CVGIMDKDQI QKGKAAGVGN ALIYVGAKTG RDGINGASFA SGDFSDEEAA 250 260 270 280 290 300 DRSAVQVGDP FMEKLLMDAC LEITGHHQEA LVGIQDMGAA GLVSSSVEMA GKANSGMVLD 310 320 330 340 350 360 LDLIPQREAE MTPFEIMLSE SQERMLLCVR AGFEQEVLAV FADYDLDAAI VGHVIAGHQY 370 380 390 400 410 420 QLYHHGKLVC DVPVSSLTDD APIYHQQGKM PKRLAQPAAD FDPIITDPVQ IWTDMMAMPT 430 440 450 460 470 480 IADKSSLYKR YDAQVQTNTV VLPGSDAAVI RIRGTHRALA MTTDSKDVTC ILIAGGCGNE 490 500 510 520 530 540 CWLKRARNLV ASGAEPLGIT DCLNFGDPTK PEAFYELAEA AKGIIAATKA FNAPVISGNV 550 560 570 580 590 600 SLYNETNGEA IYPTPMIGMV GLIEDLSTIT TAAFKQADDL IYLVGETHGD FNGSELQKLQ 610 620 630 640 650 660 TGEVTGKLFD FDLEAEKQHQ HFVLKAIREH LITAAHDLSD GGLLVALAEM GFDAQLGAQI 670 680 690 700 710 720 NVTLPTAWGF SETQGRFLLT VSPENQAAFE ALHGPAQLIG RVQAPPEFEV TTVNQHFSAS 730 740 LQQLQTAFEE ALPCHMNQKA

References

[1]	"Isolation and complete sequence of the purL gene encoding FGAM synthase II in Lactobacillus casei." Gu Z.-M., Martindale D.W., Lee B.H. Gene 119:123-126(1992) [PubMed: 1398079] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	Erratum Gu Z.-M., Martindale D.W., Lee B.H. Gene 133:147-147(1993) [PubMed: 8224889] [Abstract] Cited for: SEQUENCE REVISION.

Cross-references

Sequence databases
EMBL GenBank DDBJ	M85265 Genomic DNA. Translation: AAC36947.1.
PIR	JC1290.
3D structure databases
ProteinModelPortal	P35852.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Family and domain databases
HAMAP	MF_00420. PurL_2. [Tree]
InterPro	IPR000728. AIR_synth. IPR010918. AIR_synth_C. IPR010074. PRibForGlyAmidine_synth_II. IPR016188. PurM_N-like. [Graphical view]
Pfam	PF00586. AIRS. 2 hits. PF02769. AIRS_C. 2 hits. [Graphical view]
SUPFAM	SSF56042. AIR_synth_C. 2 hits. SSF55326. PurM_N-like. 2 hits.
TIGRFAMs	TIGR01736. FGAM_synth_II. 1 hit.
ProtoNet	Search...

Entry information

Entry name

PURL_LACCA

Accession

Primary (citable) accession number: P35852

Entry history

Integrated into UniProtKB/Swiss-Prot:	June 1, 1994
Last sequence update:	February 1, 1996
Last modified:	January 25, 2012
This is version 70 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program