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P35846

- FOLR1_MOUSE

UniProt

P35846 - FOLR1_MOUSE

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Protein

Folate receptor alpha

Gene

Folr1

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli

Functioni

Binds to folate and reduced folic acid derivatives and mediates delivery of 5-methyltetrahydrofolate and folate analogs into the interior of cells. Has high affinity for folate and folic acid analogs at neutral pH. Exposure to slightly acidic pH after receptor endocytosis triggers a conformation change that strongly reduces its affinity for folates and mediates their release. Required for normal embryonic development and normal cell proliferation. Required for renal folate reabsorption.6 Publications

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei101 – 1011FolateBy similarity
Binding sitei105 – 1051FolateBy similarity
Binding sitei194 – 1941FolateBy similarity

GO - Molecular functioni

  1. folic acid binding Source: UniProtKB
  2. folic acid transporter activity Source: UniProtKB
  3. receptor activity Source: MGI

GO - Biological processi

  1. folic acid metabolic process Source: MGI
  2. folic acid transport Source: UniProtKB
  3. posttranslational protein targeting to membrane Source: MGI
Complete GO annotation...

Keywords - Molecular functioni

Receptor

Keywords - Biological processi

Transport

Keywords - Ligandi

Folate-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Folate receptor alpha
Short name:
FR-alpha
Alternative name(s):
Folate receptor 1
Folate-binding protein 1
Gene namesi
Name:Folr1
Synonyms:Fbp1, Folbp1
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589: Chromosome 7

Organism-specific databases

MGIiMGI:95568. Folr1.

Subcellular locationi

Cell membrane; Lipid-anchorGPI-anchor. Secreted By similarity. Cytoplasmic vesicle By similarity. Cytoplasmic vesicleclathrin-coated vesicle By similarity. Endosome By similarity. Apical cell membrane
Note: Endocytosed into cytoplasmic vesicles and then recycled to the cell membrane (By similarity). Detected at proximal tubule apical membranes.By similarity

GO - Cellular componenti

  1. anchored component of external side of plasma membrane Source: UniProtKB
  2. brush border membrane Source: Ensembl
  3. cytoplasmic vesicle Source: UniProtKB-KW
  4. endosome Source: UniProtKB-KW
  5. extracellular vesicular exosome Source: Ensembl
  6. membrane Source: MGI
Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Cytoplasmic vesicle, Endosome, Membrane, Secreted

Pathology & Biotechi

Disruption phenotypei

Embryonic lethality at about 10.5 dpc, due to gross developmental defects, including defects of neural tube closure, craniofacial defects and defects in heart development. Embryos can be rescued by maternal folate supplementation.5 Publications

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 2424Sequence AnalysisAdd
BLAST
Chaini25 – 232208Folate receptor alphaPRO_0000008804Add
BLAST
Propeptidei233 – 25523Removed in mature formBy similarityPRO_0000008805Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Disulfide bondi35 ↔ 63By similarity
Disulfide bondi55 ↔ 103By similarity
Disulfide bondi64 ↔ 107By similarity
Glycosylationi67 – 671N-linked (GlcNAc...)Sequence Analysis
Disulfide bondi87 ↔ 173By similarity
Disulfide bondi94 ↔ 144By similarity
Disulfide bondi133 ↔ 207By similarity
Disulfide bondi137 ↔ 187By similarity
Disulfide bondi150 ↔ 167By similarity
Glycosylationi159 – 1591N-linked (GlcNAc...)Sequence Analysis
Glycosylationi199 – 1991N-linked (GlcNAc...)Sequence Analysis
Lipidationi232 – 2321GPI-anchor amidated serineBy similarity

Post-translational modificationi

The secreted form is derived from the membrane-bound form either by cleavage of the GPI anchor, or/and by proteolysis catalyzed by a metalloprotease.By similarity

Keywords - PTMi

Disulfide bond, Glycoprotein, GPI-anchor, Lipoprotein

Proteomic databases

PaxDbiP35846.
PRIDEiP35846.

Expressioni

Tissue specificityi

Detected in kidney proximal tubules (at protein level).1 Publication

Gene expression databases

BgeeiP35846.
CleanExiMM_FBP1.
MM_FOLR1.
ExpressionAtlasiP35846. baseline and differential.
GenevestigatoriP35846.

Interactioni

Protein-protein interaction databases

IntActiP35846. 1 interaction.
MINTiMINT-4130923.

Structurei

3D structure databases

ProteinModelPortaliP35846.
SMRiP35846. Positions 28-233.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni122 – 1265Folate bindingBy similarity
Regioni155 – 1606Folate bindingBy similarity

Sequence similaritiesi

Belongs to the folate receptor family.Curated

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiNOG26606.
GeneTreeiENSGT00390000010470.
HOGENOMiHOG000006539.
HOVERGENiHBG039612.
InParanoidiP35846.
KOiK13649.
OMAiNWTSGFN.
OrthoDBiEOG7K6PW3.
TreeFamiTF328532.

Family and domain databases

InterProiIPR004269. Folate_rcpt.
IPR018143. Folate_rcpt-like.
[Graphical view]
PANTHERiPTHR10517. PTHR10517. 1 hit.
PfamiPF03024. Folate_rec. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P35846 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MAHLMTVQLL LLVMWMAECA QSRATRARTE LLNVCMDAKH HKEKPGPEDN
60 70 80 90 100
LHDQCSPWKT NSCCSTNTSQ EAHKDISYLY RFNWNHCGTM TSECKRHFIQ
110 120 130 140 150
DTCLYECSPN LGPWIQQVDQ SWRKERILDV PLCKEDCQQW WEDCQSSFTC
160 170 180 190 200
KSNWHKGWNW SSGHNECPVG ASCHPFTFYF PTSAALCEEI WSHSYKLSNY
210 220 230 240 250
SRGSGRCIQM WFDPAQGNPN EEVARFYAEA MSGAGFHGTW PLLCSLSLVL

LWVIS
Length:255
Mass (Da):29,449
Last modified:December 11, 2013 - v2
Checksum:iA12ACA978BC8E134
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti236 – 2361F → L in AAA37595. (PubMed:1894617)Curated

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
M64782 mRNA. Translation: AAA37595.1.
AF096319 mRNA. Translation: AAD19353.1.
AC167240 Genomic DNA. No translation available.
CH466531 Genomic DNA. Translation: EDL16530.1.
CH466531 Genomic DNA. Translation: EDL16532.1.
CH466531 Genomic DNA. Translation: EDL16533.1.
CH466531 Genomic DNA. Translation: EDL16534.1.
CH466531 Genomic DNA. Translation: EDL16535.1.
CH466531 Genomic DNA. Translation: EDL16536.1.
CH466531 Genomic DNA. Translation: EDL16537.1.
CH466531 Genomic DNA. Translation: EDL16538.1.
BC138781 mRNA. Translation: AAI38782.1.
BC138795 mRNA. Translation: AAI38796.1.
BC145414 mRNA. Translation: AAI45415.1.
M97700 Genomic DNA. Translation: AAA37596.1.
M97701 Genomic DNA. Translation: AAA37598.1.
CCDSiCCDS21517.1.
PIRiA40969.
RefSeqiNP_001239481.1. NM_001252552.1.
NP_001239482.1. NM_001252553.1.
NP_001239483.1. NM_001252554.1.
NP_032060.2. NM_008034.3.
XP_006507423.1. XM_006507360.1.
XP_006507424.1. XM_006507361.1.
XP_006507425.1. XM_006507362.1.
XP_006507426.1. XM_006507363.1.
XP_006507427.1. XM_006507364.1.
XP_006507428.1. XM_006507365.1.
XP_006507429.1. XM_006507366.1.
XP_006507430.1. XM_006507367.1.
XP_006507431.1. XM_006507368.1.
XP_006507432.1. XM_006507369.1.
XP_006507433.1. XM_006507370.1.
UniGeneiMm.2135.
Mm.490265.

Genome annotation databases

EnsembliENSMUST00000001882; ENSMUSP00000001882; ENSMUSG00000001827.
ENSMUST00000106981; ENSMUSP00000102594; ENSMUSG00000001827.
ENSMUST00000106982; ENSMUSP00000102595; ENSMUSG00000001827.
ENSMUST00000106983; ENSMUSP00000102596; ENSMUSG00000001827.
ENSMUST00000106985; ENSMUSP00000102598; ENSMUSG00000001827.
ENSMUST00000106986; ENSMUSP00000102599; ENSMUSG00000001827.
ENSMUST00000123321; ENSMUSP00000114167; ENSMUSG00000001827.
GeneIDi14275.
KEGGimmu:14275.
UCSCiuc009ipm.1. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
M64782 mRNA. Translation: AAA37595.1 .
AF096319 mRNA. Translation: AAD19353.1 .
AC167240 Genomic DNA. No translation available.
CH466531 Genomic DNA. Translation: EDL16530.1 .
CH466531 Genomic DNA. Translation: EDL16532.1 .
CH466531 Genomic DNA. Translation: EDL16533.1 .
CH466531 Genomic DNA. Translation: EDL16534.1 .
CH466531 Genomic DNA. Translation: EDL16535.1 .
CH466531 Genomic DNA. Translation: EDL16536.1 .
CH466531 Genomic DNA. Translation: EDL16537.1 .
CH466531 Genomic DNA. Translation: EDL16538.1 .
BC138781 mRNA. Translation: AAI38782.1 .
BC138795 mRNA. Translation: AAI38796.1 .
BC145414 mRNA. Translation: AAI45415.1 .
M97700 Genomic DNA. Translation: AAA37596.1 .
M97701 Genomic DNA. Translation: AAA37598.1 .
CCDSi CCDS21517.1.
PIRi A40969.
RefSeqi NP_001239481.1. NM_001252552.1.
NP_001239482.1. NM_001252553.1.
NP_001239483.1. NM_001252554.1.
NP_032060.2. NM_008034.3.
XP_006507423.1. XM_006507360.1.
XP_006507424.1. XM_006507361.1.
XP_006507425.1. XM_006507362.1.
XP_006507426.1. XM_006507363.1.
XP_006507427.1. XM_006507364.1.
XP_006507428.1. XM_006507365.1.
XP_006507429.1. XM_006507366.1.
XP_006507430.1. XM_006507367.1.
XP_006507431.1. XM_006507368.1.
XP_006507432.1. XM_006507369.1.
XP_006507433.1. XM_006507370.1.
UniGenei Mm.2135.
Mm.490265.

3D structure databases

ProteinModelPortali P35846.
SMRi P35846. Positions 28-233.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

IntActi P35846. 1 interaction.
MINTi MINT-4130923.

Proteomic databases

PaxDbi P35846.
PRIDEi P35846.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENSMUST00000001882 ; ENSMUSP00000001882 ; ENSMUSG00000001827 .
ENSMUST00000106981 ; ENSMUSP00000102594 ; ENSMUSG00000001827 .
ENSMUST00000106982 ; ENSMUSP00000102595 ; ENSMUSG00000001827 .
ENSMUST00000106983 ; ENSMUSP00000102596 ; ENSMUSG00000001827 .
ENSMUST00000106985 ; ENSMUSP00000102598 ; ENSMUSG00000001827 .
ENSMUST00000106986 ; ENSMUSP00000102599 ; ENSMUSG00000001827 .
ENSMUST00000123321 ; ENSMUSP00000114167 ; ENSMUSG00000001827 .
GeneIDi 14275.
KEGGi mmu:14275.
UCSCi uc009ipm.1. mouse.

Organism-specific databases

CTDi 2348.
MGIi MGI:95568. Folr1.

Phylogenomic databases

eggNOGi NOG26606.
GeneTreei ENSGT00390000010470.
HOGENOMi HOG000006539.
HOVERGENi HBG039612.
InParanoidi P35846.
KOi K13649.
OMAi NWTSGFN.
OrthoDBi EOG7K6PW3.
TreeFami TF328532.

Miscellaneous databases

NextBioi 285649.
PROi P35846.
SOURCEi Search...

Gene expression databases

Bgeei P35846.
CleanExi MM_FBP1.
MM_FOLR1.
ExpressionAtlasi P35846. baseline and differential.
Genevestigatori P35846.

Family and domain databases

InterProi IPR004269. Folate_rcpt.
IPR018143. Folate_rcpt-like.
[Graphical view ]
PANTHERi PTHR10517. PTHR10517. 1 hit.
Pfami PF03024. Folate_rec. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Characterization of two cDNAs encoding folate-binding proteins from L1210 murine leukemia cells. Increased expression associated with a genomic rearrangement."
    Brigle K.E., Westin E.H., Houghton M.T., Goldman I.D.
    J. Biol. Chem. 266:17243-17249(1991) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, SUBCELLULAR LOCATION.
  2. "Retinoic acid-dependent upregulation of mouse folate receptor-alpha expression in embryonic stem cells, and conservation of alternative splicing patterns."
    Bolton J.A., Wood S.A., Kennedy D., Don R.H., Mattick J.S.
    Gene 230:215-224(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], INDUCTION.
  3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: C57BL/6J.
  4. Mural R.J., Adams M.D., Myers E.W., Smith H.O., Venter J.C.
    Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  5. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Lung.
  6. "Insertion of an intracisternal A particle within the 5'-regulatory region of a gene encoding folate-binding protein in L1210 leukemia cells in response to low folate selection. Association with increased protein expression."
    Brigle K.E., Westin E.H., Houghton M.T., Goldman I.D.
    J. Biol. Chem. 267:22351-22355(1992) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-54.
  7. "Mice lacking the folic acid-binding protein Folbp1 are defective in early embryonic development."
    Piedrahita J.A., Oetama B., Bennett G.D., van Waes J., Kamen B.A., Richardson J., Lacey S.W., Anderson R.G., Finnell R.H.
    Nat. Genet. 23:228-232(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: DISRUPTION PHENOTYPE, FUNCTION.
  8. Cited for: DISRUPTION PHENOTYPE, FUNCTION.
  9. "Developmental consequences of abnormal folate transport during murine heart morphogenesis."
    Tang L.S., Wlodarczyk B.J., Santillano D.R., Miranda R.C., Finnell R.H.
    Birth Defects Res. A Clin. Mol. Teratol. 70:449-458(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: DISRUPTION PHENOTYPE, FUNCTION.
  10. "Renal tubular reabsorption of folate mediated by folate binding protein 1."
    Birn H., Spiegelstein O., Christensen E.I., Finnell R.H.
    J. Am. Soc. Nephrol. 16:608-615(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: DISRUPTION PHENOTYPE, FUNCTION, SUBCELLULAR LOCATION, TISSUE SPECIFICITY.
  11. Cited for: DISRUPTION PHENOTYPE, FUNCTION.

Entry informationi

Entry nameiFOLR1_MOUSE
AccessioniPrimary (citable) accession number: P35846
Secondary accession number(s): Q9R222
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 1, 1994
Last sequence update: December 11, 2013
Last modified: October 29, 2014
This is version 96 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3