Subtilisin NAT precursor - Bacillus subtilis subsp. natto

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Reviewed, UniProtKB/Swiss-Prot P35835 (SUBN_BACNA)

Last modified June 16, 2009. Version 58. History...

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Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents

Names and origin

Protein names

Recommended name:
    Subtilisin NAT
    EC=3.4.21.62
Alternative name(s):
    Nattokinase

Gene names

Name:

aprN

Organism

Bacillus subtilis subsp. natto

Taxonomic identifier

Taxonomic lineage

Bacteria › Firmicutes › Bacillales › Bacillaceae › Bacillus

Protein attributes

Sequence length	381 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Subtilisin is an extracellular alkaline serine protease, it catalyzes the hydrolysis of proteins and peptide amides. Subtilisin NAT also has fibrinolytic activity.
Catalytic activity	Hydrolysis of proteins with broad specificity for peptide bonds, and a preference for a large uncharged residue in P1. Hydrolyzes peptide amides.
Cofactor	Binds 2 calcium ions per subunit By similarity.
Subunit structure	Monomer.
Subcellular location	Secreted.
Miscellaneous	Secretion of subtilisin is associated with onset of sporulation, and many mutations which block sporulation at early stages affect expression levels of subtilisin. However, subtilisin is not necessary for normal sporulation. In Japan this enzyme, isolated from a process of fermentation involving boiled soybeans and Bacillus natto, is used as a food supplement because it is reported to reduce and prevent blood clotting.
Sequence similarities	Belongs to the peptidase S8 family.
biophysicochemical properties	Kinetic parameters: K_M=0.48 mM for Suc-Ala-Ala-Pro-Phe-pNA

Ontologies

Keywords
Biological process	Sporulation
Cellular component	Secreted
Domain	Signal
Ligand	Calcium Metal-binding
Molecular function	Hydrolase Protease Serine protease
PTM	Zymogen
Technical term	Direct protein sequencing
Gene Ontology (GO)
Biological process	negative regulation of catalytic activity Inferred from electronic annotation. Source: InterPro proteolysis Inferred from electronic annotation. Source: InterPro sporulation resulting in formation of a cellular spore Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	extracellular region Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	calcium ion binding Inferred from electronic annotation. Source: UniProtKB-KW identical protein binding Inferred from electronic annotation. Source: InterPro serine-type endopeptidase activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Signal peptide

23

Potential

Propeptide

83

PRO_0000027175

Chain

275

Subtilisin NAT

PRO_0000027176

Sites

Active site

1

Charge relay system By similarity

Active site

1

Charge relay system By similarity

Active site

1

Charge relay system By similarity

Metal binding

1

Calcium 1 By similarity

Metal binding

1

Calcium 1 By similarity

Metal binding

1

Calcium 1; via carbonyl oxygen By similarity

Metal binding

1

Calcium 1 By similarity

Metal binding

1

Calcium 1; via carbonyl oxygen By similarity

Metal binding

1

Calcium 1; via carbonyl oxygen By similarity

Metal binding

1

Calcium 2; via carbonyl oxygen By similarity

Metal binding

1

Calcium 2; via carbonyl oxygen By similarity

Metal binding

1

Calcium 2; via carbonyl oxygen By similarity

Sequences

Sequence

Length

Mass (Da)

Tools

P35835-1 [UniParc].

Last modified June 1, 1994. Version 1.
Checksum: DAED4B16ED1BA092
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381

39,507

        10         20         30         40         50         60 
MRSKKLWISL LFALTLIFTM AFSNMSAQAA GKSSTEKKYI VGFKQTMSAM SSAKKKDVIS 

        70         80         90        100        110        120 
EKGGKVQKQF KYVNAAAATL DEKAVKELKK DPSVAYVEED HIAHEYAQSV PYGISQIKAP 

       130        140        150        160        170        180 
ALHSQGYTGS NVKVAVIDSG IDSSHPDLNV RGGASFVPSE TNPYQDGSSH GTHVAGTIAA 

       190        200        210        220        230        240 
LNNSIGVLGV APSASLYAVK VLDSTGSGQY SWIINGIEWA ISNNMDVINM SLGGPTGSTA 

       250        260        270        280        290        300 
LKTVVDKAVS SGIVVAAAAG NEGSSGSTST VGYPAKYPST IAVGAVNSSN QRASFSSVGS 

       310        320        330        340        350        360 
ELDVMAPGVS IQSTLPGGTY GAYNGTSMAT PHVAGAAALI LSKHPTWTNA QVRDRLESTA 

       370        380 
TYLGNSFYYG KGLINVQAAA Q

References

[1]	"Nucleotide sequence of the subtilisin NAT gene, aprN, of Bacillus subtilis (natto)." Nakamura T., Yamagata Y., Ichishima E. Biosci. Biotechnol. Biochem. 56:1869-1871(1992) [PubMed: 1369081] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: NC2-1.
[2]	"Purification and characterization of a strong fibrinolytic enzyme (nattokinase) in the vegetable cheese natto, a popular soybean fermented food in Japan." Fujita M., Nomura K., Hong K., Ito Y., Asada A., Nishimuro S. Biochem. Biophys. Res. Commun. 197:1340-1347(1993) [PubMed: 8280151] [Abstract] Cited for: PROTEIN SEQUENCE OF 107-381, CHARACTERIZATION.

Cross-references

Sequence databases
	D25319 Genomic DNA. Translation: BAA04989.1. S51909 Genomic DNA. Translation: AAC60424.1.
PIR	JH0778.
3D structure databases
HSSP	HSSP built from PDB template 1MEE based on UniProtKB P07518.
SMR	P35835. Positions 36-106, 107-381.
ModBase	Search...
Protein family/group databases
MEROPS	I09.001. S08.044.
Enzyme and pathway databases
BRENDA	3.4.21.62. 298357.
Family and domain databases
InterPro	IPR000209. Pept_S8_S53. IPR015500. Peptidase_S8_subtilisin-rel. IPR010259. Prot_inh_S8A. [Graphical view]
Gene3D	G3DSA:3.40.50.200. Pept_S8_S53. 1 hit.
PANTHER	PTHR10795. SubtilSerProt. 1 hit.
Pfam	PF05922. Inhibitor_I9. 1 hit. PF00082. Peptidase_S8. 1 hit. [Graphical view]
PRINTS	PR00723. SUBTILISIN.
PROSITE	PS00136. SUBTILASE_ASP. 1 hit. PS00137. SUBTILASE_HIS. 1 hit. PS00138. SUBTILASE_SER. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

SUBN_BACNA

Accession

Primary (citable) accession number: P35835

Entry history

Integrated into UniProtKB/Swiss-Prot:	June 1, 1994
Last sequence update:	June 1, 1994
Last modified:	June 16, 2009
This is version 58 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)

Relevant documents

Peptidase families

Classification of peptidase families and list of entries

SIMILARITY comments

Index of protein domains and families

Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents