P35833 (CSF3_BOVIN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Granulocyte colony-stimulating factor
Short name=G-CSF

Gene names

Name:	CSF3
Synonyms:	GCSF

Organism

Bos taurus (Bovine) [Reference proteome]

Taxonomic identifier

9913 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Laurasiatheria › Cetartiodactyla › Ruminantia › Pecora › Bovidae › Bovinae › Bos

Protein attributes

Sequence length	195 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Granulocyte/macrophage colony-stimulating factors are cytokines that act in hematopoiesis by controlling the production, differentiation, and function of 2 related white cell populations of the blood, the granulocytes and the monocytes-macrophages. This CSF induces granulocytes.
Subunit structure	Monomer.
Subcellular location	Secreted.
Post-translational modification	O-glycosylated.
Sequence similarities	Belongs to the IL-6 superfamily.

Ontologies

Keywords
Cellular component	Secreted
Domain	Signal
Molecular function	Cytokine Growth factor
PTM	Disulfide bond Glycoprotein
Technical term	3D-structure Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	immune response Inferred from electronic annotation. Source: InterPro
Cellular_component	extracellular space Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Signal peptide

1 – 21

21

Potential

Chain

22 – 195

174

Granulocyte colony-stimulating factor

PRO_0000015568

Amino acid modifications

Glycosylation

154

1

O-linked (GalNAc...) By similarity

Disulfide bond

57 ↔ 63

Disulfide bond

85 ↔ 95

Experimental info

Sequence conflict

93 – 94

2

TS → RG Ref.2

Secondary structure

1

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

195

Helix Strand Turn

Details...

Helix

32 – 60

29

Helix

65 – 69

5

Helix

72 – 75

4

Helix

83 – 85

3

Turn

87 – 89

3

Helix

92 – 112

21

Turn

113 – 115

3

Turn

118 – 120

3

Helix

121 – 145

25

Helix

164 – 191

28

Sequences

	Sequence		Length	Mass (Da)
	P35833 [UniParc]. Last modified April 27, 2001. Version 2. Checksum: 8C06119E4ADFBA73	FASTA	195	21,431
	10 20 30 40 50 60 MKLMVLQLLL WHSALWTVHE ATPLGPARSL PQSFLLKCLE QVRKIQADGA ELQERLCAAH 70 80 90 100 110 120 KLCHPEELML LRHSLGIPQA PLSSCSSQSL QLTSCLNQLH GGLFLYQGLL QALAGISPEL 130 140 150 160 170 180 APTLDTLQLD VTDFATNIWL QMEDLGAAPA VQPTQGAMPT FTSAFQRRAG GVLVASQLHR 190 FLELAYRGLR YLAEP

References

[1]	"Cloning, sequencing, and analysis of cDNA encoding bovine granulocyte-colony stimulating factor." Heidari M., Kehrli M.E. Jr. Vet. Immunol. Immunopathol. 73:183-191(2000) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: Holstein.
[2]	"Crystal structure of canine and bovine granulocyte-colony stimulating factor (G-CSF)." Lovejoy B., Cascio D., Eisenberg D. J. Mol. Biol. 234:640-653(1993) [PubMed] [Europe PMC] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (1.7 ANGSTROMS).

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

AF092533 mRNA. Translation: AAD16102.1.

IPI

IPI00699490.

RefSeq

NP_776453.1. NM_174028.1.

UniGene

Bt.4660.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
1BGC	X-ray	1.70	A	22-195	[»]

ProteinModelPortal

P35833.

SMR

P35833. Positions 29-193.

ModBase

Search...

Protein-protein interaction databases

STRING

9913.ENSBTAP00000028610.

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Genome annotation databases

Ensembl

ENSBTAT00000028610; ENSBTAP00000028610; ENSBTAG00000021462.

GeneID

281096.

KEGG

bta:281096.

Organism-specific databases

CTD

1440.

Phylogenomic databases

eggNOG

NOG47652.

GeneTree

ENSGT00390000017328.

HOGENOM

HOG000015371.

HOVERGEN

HBG005411.

InParanoid

P35833.

KO

K05423.

OMA

CHPEELV.

OrthoDB

EOG4SBDZV.

Family and domain databases

Gene3D

1.20.1250.10. 1 hit.

InterPro

IPR009079. 4_helix_cytokine-like_core.
IPR012351. 4_helix_cytokine_core.
IPR003573. IL6_MGF_GCSF.
[Graphical view]

Pfam

PF00489. IL6. 1 hit.
[Graphical view]

PIRSF

PIRSF001935. IL6_MGF_GCSF. 1 hit.

PRINTS

PR00433. IL6GCSFMGF.

SMART

SM00126. IL6. 1 hit.
[Graphical view]

SUPFAM

SSF47266. 4_helix_cytokine. 1 hit.

PROSITE

PS00254. INTERLEUKIN_6. 1 hit.
[Graphical view]

ProtoNet

Search...

Other

EvolutionaryTrace

P35833.

NextBio

20805170.

Entry information

Entry name

CSF3_BOVIN

Accession

Primary (citable) accession number: P35833
Secondary accession number(s): Q9TV89

Entry history

Integrated into UniProtKB/Swiss-Prot:	June 1, 1994
Last sequence update:	April 27, 2001
Last modified:	April 3, 2013
This is version 85 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program