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Protein

Tyrosine-protein phosphatase non-receptor type 12

Gene

Ptpn12

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Dephosphorylates a range of proteins, and thereby regulates cellular signaling cascades (PubMed:17070019). Dephosphorylates cellular tyrosine kinases, such as ERBB2 and PTK2B/PYK2, and thereby regulates signaling via ERBB2 and PTK2B/PYK2. Selectively dephosphorylates ERBB2 phosphorylated at 'Tyr-1112', 'Tyr-1196', and/or 'Tyr-1248' (By similarity).By similarity1 Publication

Catalytic activityi

Protein tyrosine phosphate + H2O = protein tyrosine + phosphate.PROSITE-ProRule annotation1 Publication

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei199SubstrateBy similarity1
Active sitei231Phosphocysteine intermediatePROSITE-ProRule annotation1
Binding sitei278SubstrateBy similarity1

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Protein phosphatase

Enzyme and pathway databases

ReactomeiR-MMU-1250196. SHC1 events in ERBB2 signaling.
R-MMU-182971. EGFR downregulation.
R-MMU-186797. Signaling by PDGF.
R-MMU-8863795. Downregulation of ERBB2 signaling.

Names & Taxonomyi

Protein namesi
Recommended name:
Tyrosine-protein phosphatase non-receptor type 12 (EC:3.1.3.481 Publication)
Alternative name(s):
MPTP-PEST
Protein-tyrosine phosphatase P19
Short name:
P19-PTP
Gene namesi
Name:Ptpn12
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 5

Organism-specific databases

MGIiMGI:104673. Ptpn12.

Subcellular locationi

GO - Cellular componenti

  • cell projection Source: UniProtKB-KW
  • cytoplasm Source: MGI
  • cytosol Source: MGI
  • focal adhesion Source: UniProtKB-SubCell
  • plasma membrane Source: MGI
  • podosome Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Cell junction, Cell projection, Cytoplasm

Pathology & Biotechi

Disruption phenotypei

Complete embryonic lethality at 9.5 to 10.5 dpc. Heterozygous mice have no obvious phenotype.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000947721 – 775Tyrosine-protein phosphatase non-receptor type 12Add BLAST775

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei1N-acetylmethionineBy similarity1
Modified residuei19PhosphoserineBy similarity1
Modified residuei331PhosphoserineCombined sources1
Modified residuei434PhosphoserineCombined sources1
Modified residuei448PhosphoserineBy similarity1
Modified residuei467PhosphoserineBy similarity1
Modified residuei519PhosphothreonineBy similarity1
Modified residuei550PhosphoserineCombined sources1
Modified residuei567PhosphoserineBy similarity1
Modified residuei569PhosphothreonineBy similarity1
Modified residuei596PhosphoserineCombined sources1
Modified residuei598PhosphothreonineCombined sources1
Modified residuei603PhosphoserineCombined sources1
Modified residuei606PhosphoserineCombined sources1
Modified residuei608PhosphoserineCombined sources1
Modified residuei613PhosphoserineBy similarity1
Modified residuei673PhosphoserineBy similarity1
Modified residuei748PhosphoserineCombined sources1

Post-translational modificationi

Phosphorylated by STK24/MST3 and this results in inhibition of its activity.By similarity

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

EPDiP35831.
MaxQBiP35831.
PaxDbiP35831.
PeptideAtlasiP35831.
PRIDEiP35831.

PTM databases

iPTMnetiP35831.
PhosphoSitePlusiP35831.

Expressioni

Gene expression databases

BgeeiENSMUSG00000028771.
ExpressionAtlasiP35831. baseline and differential.
GenevisibleiP35831. MM.

Interactioni

Subunit structurei

Interacts with PSTPIP1 and TGFB1I1 (PubMed:10092676, PubMed:11711533). Interacts with PTK2B/PYK2 (PubMed:12674328). Interacts with LPXN (PubMed:12674328, PubMed:15786712). Interacts with SORBS2; this interaction greatly enhances WASF1 dephosphorylation and might mediate partial translocation to focal adhesion sites (By similarity).By similarity4 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
Pstpip1P978145EBI-2642957,EBI-7484574
Shc1P980832EBI-2642957,EBI-300201

GO - Molecular functioni

Protein-protein interaction databases

BioGridi202478. 2 interactors.
IntActiP35831. 5 interactors.
MINTiMINT-1618529.
STRINGi10090.ENSMUSP00000030556.

Structurei

3D structure databases

ProteinModelPortaliP35831.
SMRiP35831.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini28 – 293Tyrosine-protein phosphatasePROSITE-ProRule annotationAdd BLAST266

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni231 – 237Substrate bindingBy similarity7
Regioni344 – 437Interaction with TGFB1I11 PublicationAdd BLAST94

Sequence similaritiesi

Contains 1 tyrosine-protein phosphatase domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiKOG0789. Eukaryota.
COG5599. LUCA.
GeneTreeiENSGT00770000120452.
HOGENOMiHOG000252955.
HOVERGENiHBG007666.
InParanoidiP35831.
KOiK18024.
OMAiTWSFHGP.
OrthoDBiEOG091G0B5O.
TreeFamiTF351977.

Family and domain databases

Gene3Di3.90.190.10. 1 hit.
InterProiIPR029021. Prot-tyrosine_phosphatase-like.
IPR000242. PTPase_domain.
IPR012266. Ptpn_12.
IPR016130. Tyr_Pase_AS.
IPR003595. Tyr_Pase_cat.
IPR000387. TYR_PHOSPHATASE_dom.
[Graphical view]
PfamiPF00102. Y_phosphatase. 1 hit.
[Graphical view]
PIRSFiPIRSF000932. Tyr-Ptase_nr12. 1 hit.
PRINTSiPR00700. PRTYPHPHTASE.
SMARTiSM00194. PTPc. 1 hit.
SM00404. PTPc_motif. 1 hit.
[Graphical view]
SUPFAMiSSF52799. SSF52799. 1 hit.
PROSITEiPS00383. TYR_PHOSPHATASE_1. 1 hit.
PS50056. TYR_PHOSPHATASE_2. 1 hit.
PS50055. TYR_PHOSPHATASE_PTP. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P35831-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MEQVEILRRF IQRVQAMKSP DHNGEDNFAR DFMRLRRLST KYRTEKIYPT
60 70 80 90 100
ATGEKEENVK KNRYKDILPF DHSRVKLTLK TPSQDSDYIN ANFIKGVYGP
110 120 130 140 150
KAYVATQGPL ANTVIDFWRM IWEYNVVIIV MACREFEMGR KKCERYWPLY
160 170 180 190 200
GEDPITFAPF KISCENEQAR TDYFIRTLLL EFQNESRRLY QFHYVNWPDH
210 220 230 240 250
DVPSSFDSIL DMISLMRKYQ EHEDVPICIH CSAGCGRTGA ICAIDYTWNL
260 270 280 290 300
LKAGKIPEEF NVFNLIQEMR TQRHSAVQTK EQYELVHRAI AQLFEKQLQL
310 320 330 340 350
YEIHGAQKIA DGNEITTGTM VSSIDSEKQD SPPPKPPRTR SCLVEGDAKE
360 370 380 390 400
EILQPPEPHP VPPILTPSPP SAFPTVTTVW QDSDRYHPKP VLHMASPEQH
410 420 430 440 450
PADLNRSYDK SADPMGKSES AIEHIDKKLE RNLSFEIKKV PLQEGPKSFD
460 470 480 490 500
GNTLLNRGHA IKIKSASSSV VDRTSKPQEL SAGALKVDDV SQNSCADCSA
510 520 530 540 550
AHSHRAAESS EESQSNSHTP PRPDCLPLDK KGHVTWSLHG PENATPVPDS
560 570 580 590 600
PDGKSPDNHS QTLKTVSSTP NSTAEEEAHD LTEHHNSSPL LKAPLSFTNP
610 620 630 640 650
LHSDDSDSDG GSSDGAVTRN KTSISTASAT VSPASSAESA CTRRVLPMSI
660 670 680 690 700
ARQEVAGTPH SGAEKDADVS EESPPPLPER TPESFVLADM PVRPEWHELP
710 720 730 740 750
NQEWSEQRES EGLTTSGNEK HDAGGIHTEA SADSPPAFSD KKDQITKSPA
760 770
EVTDIGFGNR CGKPKGPREP PSEWT
Length:775
Mass (Da):86,526
Last modified:July 27, 2011 - v3
Checksum:iED1A7A577CE4352C
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti110 – 111LA → FR in CAA60477 (PubMed:7772023).Curated2
Sequence conflicti128I → M in CAA60477 (PubMed:7772023).Curated1
Sequence conflicti296K → N in CAA45037 (PubMed:1590786).Curated1
Sequence conflicti310A → R in CAA60477 (PubMed:7772023).Curated1
Sequence conflicti328 – 332KQDSP → DETS in CAA45037 (PubMed:1590786).Curated5
Sequence conflicti380W → V in CAA45037 (PubMed:1590786).Curated1
Sequence conflicti414 – 415PM → QW in CAA60477 (PubMed:7772023).Curated2
Sequence conflicti606 – 607SD → WH in CAA60477 (PubMed:7772023).Curated2
Sequence conflicti642T → H in CAA60477 (PubMed:7772023).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X63440 mRNA. Translation: CAA45037.1. Sequence problems.
X86781 Genomic DNA. Translation: CAA60477.1.
AK154107 mRNA. Translation: BAE32381.1.
CH466586 Genomic DNA. Translation: EDL03216.1.
BC051980 mRNA. Translation: AAH51980.1.
CCDSiCCDS19102.1.
PIRiJH0609.
S55345.
RefSeqiNP_035333.2. NM_011203.2.
UniGeneiMm.319117.

Genome annotation databases

EnsembliENSMUST00000030556; ENSMUSP00000030556; ENSMUSG00000028771.
GeneIDi19248.
KEGGimmu:19248.
UCSCiuc008woh.1. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X63440 mRNA. Translation: CAA45037.1. Sequence problems.
X86781 Genomic DNA. Translation: CAA60477.1.
AK154107 mRNA. Translation: BAE32381.1.
CH466586 Genomic DNA. Translation: EDL03216.1.
BC051980 mRNA. Translation: AAH51980.1.
CCDSiCCDS19102.1.
PIRiJH0609.
S55345.
RefSeqiNP_035333.2. NM_011203.2.
UniGeneiMm.319117.

3D structure databases

ProteinModelPortaliP35831.
SMRiP35831.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi202478. 2 interactors.
IntActiP35831. 5 interactors.
MINTiMINT-1618529.
STRINGi10090.ENSMUSP00000030556.

PTM databases

iPTMnetiP35831.
PhosphoSitePlusiP35831.

Proteomic databases

EPDiP35831.
MaxQBiP35831.
PaxDbiP35831.
PeptideAtlasiP35831.
PRIDEiP35831.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000030556; ENSMUSP00000030556; ENSMUSG00000028771.
GeneIDi19248.
KEGGimmu:19248.
UCSCiuc008woh.1. mouse.

Organism-specific databases

CTDi5782.
MGIiMGI:104673. Ptpn12.

Phylogenomic databases

eggNOGiKOG0789. Eukaryota.
COG5599. LUCA.
GeneTreeiENSGT00770000120452.
HOGENOMiHOG000252955.
HOVERGENiHBG007666.
InParanoidiP35831.
KOiK18024.
OMAiTWSFHGP.
OrthoDBiEOG091G0B5O.
TreeFamiTF351977.

Enzyme and pathway databases

ReactomeiR-MMU-1250196. SHC1 events in ERBB2 signaling.
R-MMU-182971. EGFR downregulation.
R-MMU-186797. Signaling by PDGF.
R-MMU-8863795. Downregulation of ERBB2 signaling.

Miscellaneous databases

ChiTaRSiPtpn12. mouse.
PROiP35831.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000028771.
ExpressionAtlasiP35831. baseline and differential.
GenevisibleiP35831. MM.

Family and domain databases

Gene3Di3.90.190.10. 1 hit.
InterProiIPR029021. Prot-tyrosine_phosphatase-like.
IPR000242. PTPase_domain.
IPR012266. Ptpn_12.
IPR016130. Tyr_Pase_AS.
IPR003595. Tyr_Pase_cat.
IPR000387. TYR_PHOSPHATASE_dom.
[Graphical view]
PfamiPF00102. Y_phosphatase. 1 hit.
[Graphical view]
PIRSFiPIRSF000932. Tyr-Ptase_nr12. 1 hit.
PRINTSiPR00700. PRTYPHPHTASE.
SMARTiSM00194. PTPc. 1 hit.
SM00404. PTPc_motif. 1 hit.
[Graphical view]
SUPFAMiSSF52799. SSF52799. 1 hit.
PROSITEiPS00383. TYR_PHOSPHATASE_1. 1 hit.
PS50056. TYR_PHOSPHATASE_2. 1 hit.
PS50055. TYR_PHOSPHATASE_PTP. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiPTN12_MOUSE
AccessioniPrimary (citable) accession number: P35831
Secondary accession number(s): Q80UM4
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 1, 1994
Last sequence update: July 27, 2011
Last modified: November 30, 2016
This is version 151 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.