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P35822

- PTPRK_MOUSE

UniProt

P35822 - PTPRK_MOUSE

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Protein

Receptor-type tyrosine-protein phosphatase kappa

Gene

Ptprk

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli

Functioni

Regulation of processes involving cell contact and adhesion such as growth control, tumor invasion, and metastasis. Negative regulator of EGFR signaling pathway. Forms complexes with beta-catenin and gamma-catenin/plakoglobin. Beta-catenin may be a substrate for the catalytic activity of PTPRK/PTP-kappa.

Catalytic activityi

Protein tyrosine phosphate + H2O = protein tyrosine + phosphate.PROSITE-ProRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei1068 – 10681SubstrateBy similarity
Active sitei1100 – 11001Phosphocysteine intermediateBy similarity
Binding sitei1144 – 11441SubstrateBy similarity
Active sitei1394 – 13941Phosphocysteine intermediateBy similarity

GO - Molecular functioni

  1. protein tyrosine phosphatase activity Source: MGI

GO - Biological processi

  1. cell migration Source: Ensembl
  2. cellular response to reactive oxygen species Source: Ensembl
  3. cellular response to UV Source: Ensembl
  4. focal adhesion assembly Source: Ensembl
  5. negative regulation of cell cycle Source: Ensembl
  6. negative regulation of cell migration Source: Ensembl
  7. negative regulation of keratinocyte proliferation Source: Ensembl
  8. negative regulation of transcription, DNA-templated Source: Ensembl
  9. peptidyl-tyrosine dephosphorylation Source: GOC
  10. protein dephosphorylation Source: MGI
  11. protein localization to cell surface Source: Ensembl
  12. transforming growth factor beta receptor signaling pathway Source: Ensembl
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Protein phosphatase, Receptor

Names & Taxonomyi

Protein namesi
Recommended name:
Receptor-type tyrosine-protein phosphatase kappa (EC:3.1.3.48)
Short name:
Protein-tyrosine phosphatase kappa
Short name:
R-PTP-kappa
Gene namesi
Name:Ptprk
Synonyms:Ptpk
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589: Chromosome 10

Organism-specific databases

MGIiMGI:103310. Ptprk.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini26 – 752727ExtracellularSequence AnalysisAdd
BLAST
Transmembranei753 – 77422HelicalSequence AnalysisAdd
BLAST
Topological domaini775 – 1457683CytoplasmicSequence AnalysisAdd
BLAST

GO - Cellular componenti

  1. axon Source: MGI
  2. cell-cell junction Source: Ensembl
  3. cell surface Source: Ensembl
  4. dendrite Source: MGI
  5. integral component of membrane Source: UniProtKB-KW
  6. leading edge membrane Source: Ensembl
  7. neuronal cell body Source: MGI
  8. photoreceptor outer segment Source: MGI
Complete GO annotation...

Keywords - Cellular componenti

Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 2525Sequence AnalysisAdd
BLAST
Chaini26 – 14571432Receptor-type tyrosine-protein phosphatase kappaPRO_0000025447Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi100 – 1001N-linked (GlcNAc...)Sequence Analysis
Glycosylationi139 – 1391N-linked (GlcNAc...)1 Publication
Glycosylationi210 – 2101N-linked (GlcNAc...)Sequence Analysis
Disulfide bondi215 ↔ 269PROSITE-ProRule annotation
Glycosylationi415 – 4151N-linked (GlcNAc...)1 Publication
Glycosylationi423 – 4231N-linked (GlcNAc...)Sequence Analysis
Glycosylationi435 – 4351N-linked (GlcNAc...)Sequence Analysis
Glycosylationi461 – 4611N-linked (GlcNAc...)1 Publication
Glycosylationi551 – 5511N-linked (GlcNAc...)Sequence Analysis
Glycosylationi585 – 5851N-linked (GlcNAc...)Sequence Analysis
Glycosylationi589 – 5891N-linked (GlcNAc...)Sequence Analysis
Glycosylationi606 – 6061N-linked (GlcNAc...)Sequence Analysis
Glycosylationi689 – 6891N-linked (GlcNAc...)Sequence Analysis

Post-translational modificationi

This protein undergoes proteolytic processing.

Keywords - PTMi

Disulfide bond, Glycoprotein

Proteomic databases

MaxQBiP35822.
PaxDbiP35822.
PRIDEiP35822.

PTM databases

PhosphoSiteiP35822.

Miscellaneous databases

PMAP-CutDBP35822.

Expressioni

Tissue specificityi

High levels in liver and kidney. Lower levels in lung, brain and heart. Not seen in spleen and testis.

Developmental stagei

Developmentally regulated with highest expression found in developing areas or in areas capable of developmental plasticity.

Gene expression databases

BgeeiP35822.
CleanExiMM_PTPRK.
ExpressionAtlasiP35822. baseline and differential.
GenevestigatoriP35822.

Interactioni

Protein-protein interaction databases

IntActiP35822. 1 interaction.
MINTiMINT-4109163.

Structurei

3D structure databases

ProteinModelPortaliP35822.
SMRiP35822. Positions 32-595, 876-1456.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini30 – 193164MAMPROSITE-ProRule annotationAdd
BLAST
Domaini195 – 28086Ig-like C2-typeAdd
BLAST
Domaini293 – 38896Fibronectin type-III 1PROSITE-ProRule annotationAdd
BLAST
Domaini391 – 48797Fibronectin type-III 2PROSITE-ProRule annotationAdd
BLAST
Domaini490 – 594105Fibronectin type-III 3PROSITE-ProRule annotationAdd
BLAST
Domaini595 – 68894Fibronectin type-III 4PROSITE-ProRule annotationAdd
BLAST
Domaini899 – 1159261Tyrosine-protein phosphatase 1PROSITE-ProRule annotationAdd
BLAST
Domaini1191 – 1453263Tyrosine-protein phosphatase 2PROSITE-ProRule annotationAdd
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni1100 – 11067Substrate bindingBy similarity

Sequence similaritiesi

Contains 4 fibronectin type-III domains.PROSITE-ProRule annotation
Contains 1 MAM domain.PROSITE-ProRule annotation
Contains 2 tyrosine-protein phosphatase domains.PROSITE-ProRule annotation

Keywords - Domaini

Immunoglobulin domain, Repeat, Signal, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiCOG5599.
GeneTreeiENSGT00760000118900.
HOGENOMiHOG000049029.
HOVERGENiHBG062785.
InParanoidiP35822.
KOiK06776.
OMAiYRDGYQR.
OrthoDBiEOG70KGNP.
PhylomeDBiP35822.
TreeFamiTF312900.

Family and domain databases

Gene3Di2.60.40.10. 4 hits.
3.90.190.10. 2 hits.
InterProiIPR013320. ConA-like_dom.
IPR003961. Fibronectin_type3.
IPR007110. Ig-like_dom.
IPR013783. Ig-like_fold.
IPR003599. Ig_sub.
IPR000998. MAM_dom.
IPR029021. Prot-tyrosine_phosphatase-like.
IPR000387. Tyr/Dual-sp_Pase.
IPR016130. Tyr_Pase_AS.
IPR000242. Tyr_Pase_rcpt/non-rcpt.
[Graphical view]
PfamiPF00041. fn3. 2 hits.
PF00629. MAM. 1 hit.
PF00102. Y_phosphatase. 2 hits.
[Graphical view]
PRINTSiPR00020. MAMDOMAIN.
PR00700. PRTYPHPHTASE.
SMARTiSM00060. FN3. 3 hits.
SM00409. IG. 1 hit.
SM00137. MAM. 1 hit.
SM00194. PTPc. 2 hits.
[Graphical view]
SUPFAMiSSF49265. SSF49265. 2 hits.
SSF49899. SSF49899. 1 hit.
SSF52799. SSF52799. 2 hits.
PROSITEiPS50853. FN3. 3 hits.
PS50835. IG_LIKE. 1 hit.
PS00740. MAM_1. 1 hit.
PS50060. MAM_2. 1 hit.
PS00383. TYR_PHOSPHATASE_1. 2 hits.
PS50056. TYR_PHOSPHATASE_2. 2 hits.
PS50055. TYR_PHOSPHATASE_PTP. 2 hits.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P35822-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MDVAAAALPA FVALWLLYPW PLLGSALGQF SAGGCTFDDG PGACDYHQDL
60 70 80 90 100
YDDFEWVHVS AQEPHYLPPE MPQGSYMVVD SSNHDPGEKA RLQLPTMKEN
110 120 130 140 150
DTHCIDFSYL LYSQKGLNPG TLNILVRVNK GPLANPIWNV TGFTGRDWLR
160 170 180 190 200
AELAVSTFWP NEYQVIFEAE VSGGRSGYIA IDDIQVLSYP CDKSPHFLRL
210 220 230 240 250
GDVEVNAGQN ATFQCIATGR DAVHNKLWLQ RRNGEDIPVA QTKNINHRRF
260 270 280 290 300
AASFRLQEVT KTDQDLYRCV TQSERGSGVS NFAQLIVREP PRPIAPPQLL
310 320 330 340 350
GVGPTYLLIQ LNANSIIGDG PIILKEVEYR MTSGSWTETH AVNAPTYKLW
360 370 380 390 400
HLDPDTEYEI RVLLTRPGEG GTGLPGPPLI TRTKCAEPMR TPKTLKIAEI
410 420 430 440 450
QARRIAVDWE SLGYNITRCH TFNVTICYHY FRGHNESRAD CLDMDPKAPQ
460 470 480 490 500
HVVNHLPPYT NVSLKMILTN PEGRKESEET IIQTDEDVPG PVPVKSLQGT
510 520 530 540 550
SFENKIFLNW KEPLEPNGII TQYEVSYSSI RSFDPAVPVA GPPQTVSNLW
560 570 580 590 600
NSTHHVFMHL HPGTTYQFFI RASTVKGFGP ATAINVTTNI SAPSLPDYEG
610 620 630 640 650
VDASLNETAT TITVLLRPAQ AKGAPISAYQ IVVEQLHPHR TKREAGAMEC
660 670 680 690 700
YQVPVTYQNA LSGGAPYYFA AELPPGNLPE PAPFTVGDNR TYKGFWNPPL
710 720 730 740 750
APRKGYNIYF QAMSSVEKET KTQCVRIATK AAATEEPEVI PDPAKQTDRV
760 770 780 790 800
VKIAGISAGI LVFILLLLVV IVIVKKSKLA KKRKDAMGNT RQEMTHMVNA
810 820 830 840 850
MDRSYADQST LHAEDPLSLT FMDQHNFSPR LPNDPLVPTA VLDENHSATA
860 870 880 890 900
ESSRLLDVPR YLCEGTESPY QTGQLHPAIR VADLLQHINL MKTSDSYGFK
910 920 930 940 950
EEYESFFEGQ SASWDVAKKD QNRAKNRYGN IIAYDHSRVI LQPVEDDPSS
960 970 980 990 1000
DYINANYIDI WLYRDGYQRP SHYIATQGPV HETVYDFWRM VWQEQSACIV
1010 1020 1030 1040 1050
MVTNLVEVGR VKCYKYWPDD TEVYGDFKVT CVEMEPLAEY VVRTFTLERR
1060 1070 1080 1090 1100
GYNEIREVKQ FHFTGWPDHG VPYHATGLLS FIRRVKLSNP PSAGPIVVHC
1110 1120 1130 1140 1150
SAGAGRTGCY IVIDIMLDMA EREGVVDIYN CVKALRSRRI NMVQTEEQYI
1160 1170 1180 1190 1200
FIHDAILEAC LCGETAIPVC EFKAAYFDMI RIDSQTNSSH LKDEFQTLNS
1210 1220 1230 1240 1250
VTPRLQAEDC SIACLPRNHD KNRFMDMLPP DRCLPFLITI DGESSNYINA
1260 1270 1280 1290 1300
ALMDSYRQPA AFIVTQYPLP NTVKDFWRLV YDYGCTSIVM LNEVDLSQGC
1310 1320 1330 1340 1350
PQYWPEEGML RYGPIQVECM SCSMDCDVIN RIFRICNLTR PQEGYLMVQQ
1360 1370 1380 1390 1400
FQYLGWASHR EVPGSKRSFL KLILQVEKWQ EECEEGEGRT IIHCLNGGGR
1410 1420 1430 1440 1450
SGMFCAIGIV VEMVKRQNVV DVFHAVKTLR NSKPNMVEAP EQYRFCYDVA

LEYLESS
Length:1,457
Mass (Da):164,186
Last modified:June 1, 1994 - v1
Checksum:i19D4B99B7ECE8605
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L10106 mRNA. Translation: AAA40021.1.
CCDSiCCDS35874.1.
PIRiA48066.
RefSeqiNP_033009.1. NM_008983.2.
UniGeneiMm.332303.

Genome annotation databases

EnsembliENSMUST00000166468; ENSMUSP00000126279; ENSMUSG00000019889.
GeneIDi19272.
KEGGimmu:19272.
UCSCiuc007esk.1. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L10106 mRNA. Translation: AAA40021.1 .
CCDSi CCDS35874.1.
PIRi A48066.
RefSeqi NP_033009.1. NM_008983.2.
UniGenei Mm.332303.

3D structure databases

ProteinModelPortali P35822.
SMRi P35822. Positions 32-595, 876-1456.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

IntActi P35822. 1 interaction.
MINTi MINT-4109163.

PTM databases

PhosphoSitei P35822.

Proteomic databases

MaxQBi P35822.
PaxDbi P35822.
PRIDEi P35822.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENSMUST00000166468 ; ENSMUSP00000126279 ; ENSMUSG00000019889 .
GeneIDi 19272.
KEGGi mmu:19272.
UCSCi uc007esk.1. mouse.

Organism-specific databases

CTDi 5796.
MGIi MGI:103310. Ptprk.

Phylogenomic databases

eggNOGi COG5599.
GeneTreei ENSGT00760000118900.
HOGENOMi HOG000049029.
HOVERGENi HBG062785.
InParanoidi P35822.
KOi K06776.
OMAi YRDGYQR.
OrthoDBi EOG70KGNP.
PhylomeDBi P35822.
TreeFami TF312900.

Miscellaneous databases

ChiTaRSi Ptprk. mouse.
NextBioi 296162.
PMAP-CutDB P35822.
PROi P35822.
SOURCEi Search...

Gene expression databases

Bgeei P35822.
CleanExi MM_PTPRK.
ExpressionAtlasi P35822. baseline and differential.
Genevestigatori P35822.

Family and domain databases

Gene3Di 2.60.40.10. 4 hits.
3.90.190.10. 2 hits.
InterProi IPR013320. ConA-like_dom.
IPR003961. Fibronectin_type3.
IPR007110. Ig-like_dom.
IPR013783. Ig-like_fold.
IPR003599. Ig_sub.
IPR000998. MAM_dom.
IPR029021. Prot-tyrosine_phosphatase-like.
IPR000387. Tyr/Dual-sp_Pase.
IPR016130. Tyr_Pase_AS.
IPR000242. Tyr_Pase_rcpt/non-rcpt.
[Graphical view ]
Pfami PF00041. fn3. 2 hits.
PF00629. MAM. 1 hit.
PF00102. Y_phosphatase. 2 hits.
[Graphical view ]
PRINTSi PR00020. MAMDOMAIN.
PR00700. PRTYPHPHTASE.
SMARTi SM00060. FN3. 3 hits.
SM00409. IG. 1 hit.
SM00137. MAM. 1 hit.
SM00194. PTPc. 2 hits.
[Graphical view ]
SUPFAMi SSF49265. SSF49265. 2 hits.
SSF49899. SSF49899. 1 hit.
SSF52799. SSF52799. 2 hits.
PROSITEi PS50853. FN3. 3 hits.
PS50835. IG_LIKE. 1 hit.
PS00740. MAM_1. 1 hit.
PS50060. MAM_2. 1 hit.
PS00383. TYR_PHOSPHATASE_1. 2 hits.
PS50056. TYR_PHOSPHATASE_2. 2 hits.
PS50055. TYR_PHOSPHATASE_PTP. 2 hits.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Cloning and characterization of R-PTP-kappa, a new member of the receptor protein tyrosine phosphatase family with a proteolytically cleaved cellular adhesion molecule-like extracellular region."
    Jiang Y.P., Wang H., D'Eustachio P., Musacchio J.M., Schlessinger J., Sap J.
    Mol. Cell. Biol. 13:2942-2951(1993) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Strain: RI.
    Tissue: Brain.
  2. "Mass-spectrometric identification and relative quantification of N-linked cell surface glycoproteins."
    Wollscheid B., Bausch-Fluck D., Henderson C., O'Brien R., Bibel M., Schiess R., Aebersold R., Watts J.D.
    Nat. Biotechnol. 27:378-386(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: GLYCOSYLATION [LARGE SCALE ANALYSIS] AT ASN-139; ASN-415 AND ASN-461.

Entry informationi

Entry nameiPTPRK_MOUSE
AccessioniPrimary (citable) accession number: P35822
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 1, 1994
Last sequence update: June 1, 1994
Last modified: November 26, 2014
This is version 141 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3