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Protein

Receptor-type tyrosine-protein phosphatase kappa

Gene

Ptprk

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Regulation of processes involving cell contact and adhesion such as growth control, tumor invasion, and metastasis. Negative regulator of EGFR signaling pathway. Forms complexes with beta-catenin and gamma-catenin/plakoglobin. Beta-catenin may be a substrate for the catalytic activity of PTPRK/PTP-kappa.

Catalytic activityi

Protein tyrosine phosphate + H2O = protein tyrosine + phosphate.PROSITE-ProRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei1068SubstrateBy similarity1
Active sitei1100Phosphocysteine intermediateBy similarity1
Binding sitei1144SubstrateBy similarity1
Active sitei1394Phosphocysteine intermediateBy similarity1

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Protein phosphatase, Receptor

Enzyme and pathway databases

ReactomeiR-MMU-182971. EGFR downregulation.

Names & Taxonomyi

Protein namesi
Recommended name:
Receptor-type tyrosine-protein phosphatase kappa (EC:3.1.3.48)
Short name:
Protein-tyrosine phosphatase kappa
Short name:
R-PTP-kappa
Gene namesi
Name:Ptprk
Synonyms:Ptpk
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 10

Organism-specific databases

MGIiMGI:103310. Ptprk.

Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini26 – 752ExtracellularSequence analysisAdd BLAST727
Transmembranei753 – 774HelicalSequence analysisAdd BLAST22
Topological domaini775 – 1457CytoplasmicSequence analysisAdd BLAST683

GO - Cellular componenti

  • axon Source: MGI
  • cell-cell junction Source: MGI
  • cell surface Source: MGI
  • dendrite Source: MGI
  • integral component of membrane Source: UniProtKB-KW
  • leading edge membrane Source: MGI
  • neuronal cell body Source: MGI
  • photoreceptor outer segment Source: MGI
Complete GO annotation...

Keywords - Cellular componenti

Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 25Sequence analysisAdd BLAST25
ChainiPRO_000002544726 – 1457Receptor-type tyrosine-protein phosphatase kappaAdd BLAST1432

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi100N-linked (GlcNAc...)Sequence analysis1
Glycosylationi139N-linked (GlcNAc...)1 Publication1
Glycosylationi210N-linked (GlcNAc...)Sequence analysis1
Disulfide bondi215 ↔ 269PROSITE-ProRule annotation
Glycosylationi415N-linked (GlcNAc...)1 Publication1
Glycosylationi423N-linked (GlcNAc...)Sequence analysis1
Glycosylationi435N-linked (GlcNAc...)Sequence analysis1
Glycosylationi461N-linked (GlcNAc...)1 Publication1
Glycosylationi551N-linked (GlcNAc...)Sequence analysis1
Glycosylationi585N-linked (GlcNAc...)Sequence analysis1
Glycosylationi589N-linked (GlcNAc...)Sequence analysis1
Glycosylationi606N-linked (GlcNAc...)Sequence analysis1
Glycosylationi689N-linked (GlcNAc...)Sequence analysis1
Modified residuei868PhosphoserineCombined sources1

Post-translational modificationi

This protein undergoes proteolytic processing.

Keywords - PTMi

Disulfide bond, Glycoprotein, Phosphoprotein

Proteomic databases

MaxQBiP35822.
PaxDbiP35822.
PRIDEiP35822.

PTM databases

iPTMnetiP35822.
PhosphoSitePlusiP35822.

Miscellaneous databases

PMAP-CutDBP35822.

Expressioni

Tissue specificityi

High levels in liver and kidney. Lower levels in lung, brain and heart. Not seen in spleen and testis.

Developmental stagei

Developmentally regulated with highest expression found in developing areas or in areas capable of developmental plasticity.

Gene expression databases

BgeeiENSMUSG00000019889.
CleanExiMM_PTPRK.
GenevisibleiP35822. MM.

Interactioni

GO - Molecular functioni

Protein-protein interaction databases

BioGridi202500. 1 interactor.
IntActiP35822. 1 interactor.
MINTiMINT-4109163.
STRINGi10090.ENSMUSP00000126279.

Structurei

3D structure databases

ProteinModelPortaliP35822.
SMRiP35822.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini30 – 193MAMPROSITE-ProRule annotationAdd BLAST164
Domaini195 – 280Ig-like C2-typeAdd BLAST86
Domaini293 – 388Fibronectin type-III 1PROSITE-ProRule annotationAdd BLAST96
Domaini391 – 487Fibronectin type-III 2PROSITE-ProRule annotationAdd BLAST97
Domaini490 – 594Fibronectin type-III 3PROSITE-ProRule annotationAdd BLAST105
Domaini595 – 688Fibronectin type-III 4PROSITE-ProRule annotationAdd BLAST94
Domaini899 – 1159Tyrosine-protein phosphatase 1PROSITE-ProRule annotationAdd BLAST261
Domaini1191 – 1453Tyrosine-protein phosphatase 2PROSITE-ProRule annotationAdd BLAST263

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni1100 – 1106Substrate bindingBy similarity7

Sequence similaritiesi

Contains 4 fibronectin type-III domains.PROSITE-ProRule annotation
Contains 1 MAM domain.PROSITE-ProRule annotation
Contains 2 tyrosine-protein phosphatase domains.PROSITE-ProRule annotation

Keywords - Domaini

Immunoglobulin domain, Repeat, Signal, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG4228. Eukaryota.
COG5599. LUCA.
GeneTreeiENSGT00760000118900.
HOGENOMiHOG000049029.
HOVERGENiHBG062785.
InParanoidiP35822.
KOiK06776.
OMAiHRTKREA.
OrthoDBiEOG091G00CK.
PhylomeDBiP35822.
TreeFamiTF312900.

Family and domain databases

CDDicd00063. FN3. 2 hits.
cd06263. MAM. 1 hit.
Gene3Di2.60.40.10. 4 hits.
3.90.190.10. 2 hits.
InterProiIPR013320. ConA-like_dom.
IPR003961. FN3_dom.
IPR007110. Ig-like_dom.
IPR013783. Ig-like_fold.
IPR013098. Ig_I-set.
IPR003599. Ig_sub.
IPR000998. MAM_dom.
IPR029021. Prot-tyrosine_phosphatase-like.
IPR000242. PTPase_domain.
IPR016130. Tyr_Pase_AS.
IPR003595. Tyr_Pase_cat.
IPR000387. TYR_PHOSPHATASE_dom.
[Graphical view]
PfamiPF00041. fn3. 2 hits.
PF07679. I-set. 1 hit.
PF00629. MAM. 1 hit.
PF00102. Y_phosphatase. 2 hits.
[Graphical view]
PRINTSiPR00020. MAMDOMAIN.
PR00700. PRTYPHPHTASE.
SMARTiSM00060. FN3. 3 hits.
SM00409. IG. 1 hit.
SM00137. MAM. 1 hit.
SM00194. PTPc. 2 hits.
SM00404. PTPc_motif. 2 hits.
[Graphical view]
SUPFAMiSSF48726. SSF48726. 1 hit.
SSF49265. SSF49265. 2 hits.
SSF49899. SSF49899. 1 hit.
SSF52799. SSF52799. 2 hits.
PROSITEiPS50853. FN3. 3 hits.
PS50835. IG_LIKE. 1 hit.
PS00740. MAM_1. 1 hit.
PS50060. MAM_2. 1 hit.
PS00383. TYR_PHOSPHATASE_1. 2 hits.
PS50056. TYR_PHOSPHATASE_2. 2 hits.
PS50055. TYR_PHOSPHATASE_PTP. 2 hits.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P35822-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MDVAAAALPA FVALWLLYPW PLLGSALGQF SAGGCTFDDG PGACDYHQDL
60 70 80 90 100
YDDFEWVHVS AQEPHYLPPE MPQGSYMVVD SSNHDPGEKA RLQLPTMKEN
110 120 130 140 150
DTHCIDFSYL LYSQKGLNPG TLNILVRVNK GPLANPIWNV TGFTGRDWLR
160 170 180 190 200
AELAVSTFWP NEYQVIFEAE VSGGRSGYIA IDDIQVLSYP CDKSPHFLRL
210 220 230 240 250
GDVEVNAGQN ATFQCIATGR DAVHNKLWLQ RRNGEDIPVA QTKNINHRRF
260 270 280 290 300
AASFRLQEVT KTDQDLYRCV TQSERGSGVS NFAQLIVREP PRPIAPPQLL
310 320 330 340 350
GVGPTYLLIQ LNANSIIGDG PIILKEVEYR MTSGSWTETH AVNAPTYKLW
360 370 380 390 400
HLDPDTEYEI RVLLTRPGEG GTGLPGPPLI TRTKCAEPMR TPKTLKIAEI
410 420 430 440 450
QARRIAVDWE SLGYNITRCH TFNVTICYHY FRGHNESRAD CLDMDPKAPQ
460 470 480 490 500
HVVNHLPPYT NVSLKMILTN PEGRKESEET IIQTDEDVPG PVPVKSLQGT
510 520 530 540 550
SFENKIFLNW KEPLEPNGII TQYEVSYSSI RSFDPAVPVA GPPQTVSNLW
560 570 580 590 600
NSTHHVFMHL HPGTTYQFFI RASTVKGFGP ATAINVTTNI SAPSLPDYEG
610 620 630 640 650
VDASLNETAT TITVLLRPAQ AKGAPISAYQ IVVEQLHPHR TKREAGAMEC
660 670 680 690 700
YQVPVTYQNA LSGGAPYYFA AELPPGNLPE PAPFTVGDNR TYKGFWNPPL
710 720 730 740 750
APRKGYNIYF QAMSSVEKET KTQCVRIATK AAATEEPEVI PDPAKQTDRV
760 770 780 790 800
VKIAGISAGI LVFILLLLVV IVIVKKSKLA KKRKDAMGNT RQEMTHMVNA
810 820 830 840 850
MDRSYADQST LHAEDPLSLT FMDQHNFSPR LPNDPLVPTA VLDENHSATA
860 870 880 890 900
ESSRLLDVPR YLCEGTESPY QTGQLHPAIR VADLLQHINL MKTSDSYGFK
910 920 930 940 950
EEYESFFEGQ SASWDVAKKD QNRAKNRYGN IIAYDHSRVI LQPVEDDPSS
960 970 980 990 1000
DYINANYIDI WLYRDGYQRP SHYIATQGPV HETVYDFWRM VWQEQSACIV
1010 1020 1030 1040 1050
MVTNLVEVGR VKCYKYWPDD TEVYGDFKVT CVEMEPLAEY VVRTFTLERR
1060 1070 1080 1090 1100
GYNEIREVKQ FHFTGWPDHG VPYHATGLLS FIRRVKLSNP PSAGPIVVHC
1110 1120 1130 1140 1150
SAGAGRTGCY IVIDIMLDMA EREGVVDIYN CVKALRSRRI NMVQTEEQYI
1160 1170 1180 1190 1200
FIHDAILEAC LCGETAIPVC EFKAAYFDMI RIDSQTNSSH LKDEFQTLNS
1210 1220 1230 1240 1250
VTPRLQAEDC SIACLPRNHD KNRFMDMLPP DRCLPFLITI DGESSNYINA
1260 1270 1280 1290 1300
ALMDSYRQPA AFIVTQYPLP NTVKDFWRLV YDYGCTSIVM LNEVDLSQGC
1310 1320 1330 1340 1350
PQYWPEEGML RYGPIQVECM SCSMDCDVIN RIFRICNLTR PQEGYLMVQQ
1360 1370 1380 1390 1400
FQYLGWASHR EVPGSKRSFL KLILQVEKWQ EECEEGEGRT IIHCLNGGGR
1410 1420 1430 1440 1450
SGMFCAIGIV VEMVKRQNVV DVFHAVKTLR NSKPNMVEAP EQYRFCYDVA

LEYLESS
Length:1,457
Mass (Da):164,186
Last modified:June 1, 1994 - v1
Checksum:i19D4B99B7ECE8605
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L10106 mRNA. Translation: AAA40021.1.
CCDSiCCDS35874.1.
PIRiA48066.
RefSeqiNP_033009.1. NM_008983.2.
UniGeneiMm.332303.

Genome annotation databases

EnsembliENSMUST00000166468; ENSMUSP00000126279; ENSMUSG00000019889.
GeneIDi19272.
KEGGimmu:19272.
UCSCiuc007esk.1. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L10106 mRNA. Translation: AAA40021.1.
CCDSiCCDS35874.1.
PIRiA48066.
RefSeqiNP_033009.1. NM_008983.2.
UniGeneiMm.332303.

3D structure databases

ProteinModelPortaliP35822.
SMRiP35822.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi202500. 1 interactor.
IntActiP35822. 1 interactor.
MINTiMINT-4109163.
STRINGi10090.ENSMUSP00000126279.

PTM databases

iPTMnetiP35822.
PhosphoSitePlusiP35822.

Proteomic databases

MaxQBiP35822.
PaxDbiP35822.
PRIDEiP35822.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000166468; ENSMUSP00000126279; ENSMUSG00000019889.
GeneIDi19272.
KEGGimmu:19272.
UCSCiuc007esk.1. mouse.

Organism-specific databases

CTDi5796.
MGIiMGI:103310. Ptprk.

Phylogenomic databases

eggNOGiKOG4228. Eukaryota.
COG5599. LUCA.
GeneTreeiENSGT00760000118900.
HOGENOMiHOG000049029.
HOVERGENiHBG062785.
InParanoidiP35822.
KOiK06776.
OMAiHRTKREA.
OrthoDBiEOG091G00CK.
PhylomeDBiP35822.
TreeFamiTF312900.

Enzyme and pathway databases

ReactomeiR-MMU-182971. EGFR downregulation.

Miscellaneous databases

ChiTaRSiPtprk. mouse.
PMAP-CutDBP35822.
PROiP35822.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000019889.
CleanExiMM_PTPRK.
GenevisibleiP35822. MM.

Family and domain databases

CDDicd00063. FN3. 2 hits.
cd06263. MAM. 1 hit.
Gene3Di2.60.40.10. 4 hits.
3.90.190.10. 2 hits.
InterProiIPR013320. ConA-like_dom.
IPR003961. FN3_dom.
IPR007110. Ig-like_dom.
IPR013783. Ig-like_fold.
IPR013098. Ig_I-set.
IPR003599. Ig_sub.
IPR000998. MAM_dom.
IPR029021. Prot-tyrosine_phosphatase-like.
IPR000242. PTPase_domain.
IPR016130. Tyr_Pase_AS.
IPR003595. Tyr_Pase_cat.
IPR000387. TYR_PHOSPHATASE_dom.
[Graphical view]
PfamiPF00041. fn3. 2 hits.
PF07679. I-set. 1 hit.
PF00629. MAM. 1 hit.
PF00102. Y_phosphatase. 2 hits.
[Graphical view]
PRINTSiPR00020. MAMDOMAIN.
PR00700. PRTYPHPHTASE.
SMARTiSM00060. FN3. 3 hits.
SM00409. IG. 1 hit.
SM00137. MAM. 1 hit.
SM00194. PTPc. 2 hits.
SM00404. PTPc_motif. 2 hits.
[Graphical view]
SUPFAMiSSF48726. SSF48726. 1 hit.
SSF49265. SSF49265. 2 hits.
SSF49899. SSF49899. 1 hit.
SSF52799. SSF52799. 2 hits.
PROSITEiPS50853. FN3. 3 hits.
PS50835. IG_LIKE. 1 hit.
PS00740. MAM_1. 1 hit.
PS50060. MAM_2. 1 hit.
PS00383. TYR_PHOSPHATASE_1. 2 hits.
PS50056. TYR_PHOSPHATASE_2. 2 hits.
PS50055. TYR_PHOSPHATASE_PTP. 2 hits.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiPTPRK_MOUSE
AccessioniPrimary (citable) accession number: P35822
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 1, 1994
Last sequence update: June 1, 1994
Last modified: November 30, 2016
This is version 157 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.