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P35821

- PTN1_MOUSE

UniProt

P35821 - PTN1_MOUSE

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Protein

Tyrosine-protein phosphatase non-receptor type 1

Gene
Ptpn1
Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at protein leveli

Functioni

Tyrosine-protein phosphatase which acts as a regulator of endoplasmic reticulum unfolded protein response. Mediates dephosphorylation of EIF2AK3/PERK; inactivating the protein kinase activity of EIF2AK3/PERK. May play an important role in CKII- and p60c-src-induced signal transduction cascades. May regulate the EFNA5-EPHA3 signaling pathway which modulates cell reorganization and cell-cell repulsion. May also regulate the hepatocyte growth factor receptor signaling pathway through dephosphorylation of MET By similarity.

Catalytic activityi

Protein tyrosine phosphate + H2O = protein tyrosine + phosphate.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei181 – 1811Substrate By similarity
Active sitei215 – 2151Phosphocysteine intermediate By similarity
Binding sitei262 – 2621Substrate By similarity

GO - Molecular functioni

  1. protein tyrosine phosphatase activity Source: UniProtKB
  2. zinc ion binding Source: Ensembl

GO - Biological processi

  1. actin cytoskeleton reorganization Source: UniProtKB
  2. endoplasmic reticulum unfolded protein response Source: UniProtKB
  3. insulin receptor signaling pathway Source: MGI
  4. negative regulation of ERK1 and ERK2 cascade Source: BHF-UCL
  5. negative regulation of vascular endothelial growth factor receptor signaling pathway Source: BHF-UCL
  6. peptidyl-tyrosine dephosphorylation Source: UniProtKB
  7. peptidyl-tyrosine dephosphorylation involved in inactivation of protein kinase activity Source: BHF-UCL
  8. platelet-derived growth factor receptor-beta signaling pathway Source: UniProtKB
  9. protein dephosphorylation Source: MGI
  10. regulation of endocytosis Source: UniProtKB
  11. regulation of hepatocyte growth factor receptor signaling pathway Source: Ensembl
  12. regulation of insulin receptor signaling pathway Source: Ensembl
  13. regulation of signal transduction Source: UniProtKB
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Protein phosphatase

Enzyme and pathway databases

ReactomeiREACT_198614. Growth hormone receptor signaling.
REACT_198627. Regulation of IFNA signaling.

Names & Taxonomyi

Protein namesi
Recommended name:
Tyrosine-protein phosphatase non-receptor type 1 (EC:3.1.3.48)
Alternative name(s):
Protein-tyrosine phosphatase 1B
Short name:
PTP-1B
Protein-tyrosine phosphatase HA2
Short name:
PTP-HA2
Gene namesi
Name:Ptpn1
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589: Chromosome 2

Organism-specific databases

MGIiMGI:97805. Ptpn1.

Subcellular locationi

Endoplasmic reticulum membrane; Peripheral membrane protein; Cytoplasmic side By similarity
Note: Interacts with EPHA3 at the cell membrane By similarity.

GO - Cellular componenti

  1. cytoplasmic vesicle Source: Ensembl
  2. cytosol Source: Reactome
  3. early endosome Source: UniProtKB
  4. endoplasmic reticulum membrane Source: UniProtKB-SubCell
  5. plasma membrane Source: Ensembl
  6. sorting endosome Source: BHF-UCL
Complete GO annotation...

Keywords - Cellular componenti

Endoplasmic reticulum, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 432432Tyrosine-protein phosphatase non-receptor type 1PRO_0000094749Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei1 – 11N-acetylmethionine By similarity
Modified residuei20 – 201Phosphotyrosine By similarity
Modified residuei50 – 501Phosphoserine; by CLK1, CLK2 and PKB/AKT1 or PKB/AKT2 By similarity
Modified residuei66 – 661Phosphotyrosine; by EGFR By similarity
Modified residuei215 – 2151Cysteine persulfide By similarity
Modified residuei215 – 2151S-nitrosocysteine; in reversibly inhibited form By similarity
Modified residuei242 – 2421Phosphoserine; by CLK1 and CLK2 By similarity
Modified residuei243 – 2431Phosphoserine; by CLK1 and CLK2 By similarity

Post-translational modificationi

Ser-50 is the major site of phosphorylation as compared to Ser-242 and Ser-243. Activated by phosphorylation at Ser-50 By similarity.
S-nitrosylation of Cys-215 inactivates the enzyme activity By similarity.
Sulfhydration at Cys-215 following endoplasmic reticulum stress inactivates the enzyme activity, promoting EIF2AK3/PERK activity By similarity.

Keywords - PTMi

Acetylation, Phosphoprotein, S-nitrosylation

Proteomic databases

MaxQBiP35821.
PaxDbiP35821.
PRIDEiP35821.

PTM databases

PhosphoSiteiP35821.

Expressioni

Tissue specificityi

Most abundant in testis. Also found in kidney, spleen, muscle, liver, heart and brain.

Gene expression databases

ArrayExpressiP35821.
BgeeiP35821.
CleanExiMM_PTPN1.
GenevestigatoriP35821.

Interactioni

Subunit structurei

Interacts with EPHA3 (phosphorylated); dephosphorylates EPHA3 and may regulate its trafficking and function By similarity. Interacts with MET By similarity.

Protein-protein interaction databases

BioGridi202476. 1 interaction.
IntActiP35821. 2 interactions.
MINTiMINT-4996685.

Structurei

3D structure databases

ProteinModelPortaliP35821.
SMRiP35821. Positions 1-282.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini3 – 277275Tyrosine-protein phosphataseAdd
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni215 – 2217Substrate binding By similarity

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG5599.
HOGENOMiHOG000273908.
HOVERGENiHBG008321.
InParanoidiP35821.
KOiK05696.
OMAiMCMATVL.
OrthoDBiEOG7RV9G4.
PhylomeDBiP35821.
TreeFamiTF315897.

Family and domain databases

Gene3Di3.90.190.10. 1 hit.
InterProiIPR029021. Prot-tyrosine_phosphatase-like.
IPR012265. Ptpn1/Ptpn2.
IPR000387. Tyr/Dual-sp_Pase.
IPR016130. Tyr_Pase_AS.
IPR000242. Tyr_Pase_rcpt/non-rcpt.
[Graphical view]
PfamiPF00102. Y_phosphatase. 1 hit.
[Graphical view]
PIRSFiPIRSF000926. Tyr-Ptase_nr1. 1 hit.
PRINTSiPR00700. PRTYPHPHTASE.
SMARTiSM00194. PTPc. 1 hit.
[Graphical view]
SUPFAMiSSF52799. SSF52799. 1 hit.
PROSITEiPS00383. TYR_PHOSPHATASE_1. 1 hit.
PS50056. TYR_PHOSPHATASE_2. 1 hit.
PS50055. TYR_PHOSPHATASE_PTP. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P35821-1 [UniParc]FASTAAdd to Basket

« Hide

MEMEKEFEEI DKAGNWAAIY QDIRHEASDF PCKVAKLPKN KNRNRYRDVS    50
PFDHSRIKLH QEDNDYINAS LIKMEEAQRS YILTQGPLPN TCGHFWEMVW 100
EQKSRGVVML NRIMEKGSLK CAQYWPQQEE KEMVFDDTGL KLTLISEDVK 150
SYYTVRQLEL ENLTTKETRE ILHFHYTTWP DFGVPESPAS FLNFLFKVRE 200
SGSLSLEHGP IVVHCSAGIG RSGTFCLADT CLLLMDKRKD PSSVDIKKVL 250
LEMRRFRMGL IQTADQLRFS YLAVIEGAKF IMGDSSVQDQ WKELSREDLD 300
LPPEHVPPPP RPPKRTLEPH NGKCKELFSS HQWVSEETCG DEDSLAREEG 350
RAQSSAMHSV SSMSPDTEVR RRMVGGGLQS AQASVPTEEE LSSTEEEHKA 400
HWPSHWKPFL VNVCMATLLA TGAYLCYRVC FH 432
Length:432
Mass (Da):49,593
Last modified:November 1, 1997 - v2
Checksum:i4843D2DD4C288C48
GO

Sequence conflict

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti48 – 481D → Y in M97590. 1 Publication
Sequence conflicti173 – 1731H → P in AAA64615. 1 Publication
Sequence conflicti266 – 2672QL → HV in AAA64615. 1 Publication

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
M97590 mRNA. No translation available.
L40595 mRNA. Translation: AAA64615.1.
U24700 mRNA. Translation: AAA98605.1.
Z23057 mRNA. Translation: CAA80592.1.
BC005729 mRNA. Translation: AAH05729.1.
BC010191 mRNA. Translation: AAH10191.1.
CCDSiCCDS17107.1.
PIRiJN0317.
RefSeqiNP_035331.3. NM_011201.3.
UniGeneiMm.277916.

Genome annotation databases

EnsembliENSMUST00000029053; ENSMUSP00000029053; ENSMUSG00000027540.
GeneIDi19246.
KEGGimmu:19246.
UCSCiuc008oaj.1. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
M97590 mRNA. No translation available.
L40595 mRNA. Translation: AAA64615.1 .
U24700 mRNA. Translation: AAA98605.1 .
Z23057 mRNA. Translation: CAA80592.1 .
BC005729 mRNA. Translation: AAH05729.1 .
BC010191 mRNA. Translation: AAH10191.1 .
CCDSi CCDS17107.1.
PIRi JN0317.
RefSeqi NP_035331.3. NM_011201.3.
UniGenei Mm.277916.

3D structure databases

ProteinModelPortali P35821.
SMRi P35821. Positions 1-282.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 202476. 1 interaction.
IntActi P35821. 2 interactions.
MINTi MINT-4996685.

Chemistry

ChEMBLi CHEMBL3336.

PTM databases

PhosphoSitei P35821.

Proteomic databases

MaxQBi P35821.
PaxDbi P35821.
PRIDEi P35821.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENSMUST00000029053 ; ENSMUSP00000029053 ; ENSMUSG00000027540 .
GeneIDi 19246.
KEGGi mmu:19246.
UCSCi uc008oaj.1. mouse.

Organism-specific databases

CTDi 5770.
MGIi MGI:97805. Ptpn1.

Phylogenomic databases

eggNOGi COG5599.
HOGENOMi HOG000273908.
HOVERGENi HBG008321.
InParanoidi P35821.
KOi K05696.
OMAi MCMATVL.
OrthoDBi EOG7RV9G4.
PhylomeDBi P35821.
TreeFami TF315897.

Enzyme and pathway databases

Reactomei REACT_198614. Growth hormone receptor signaling.
REACT_198627. Regulation of IFNA signaling.

Miscellaneous databases

ChiTaRSi PTPN1. mouse.
NextBioi 296070.
PROi P35821.
SOURCEi Search...

Gene expression databases

ArrayExpressi P35821.
Bgeei P35821.
CleanExi MM_PTPN1.
Genevestigatori P35821.

Family and domain databases

Gene3Di 3.90.190.10. 1 hit.
InterProi IPR029021. Prot-tyrosine_phosphatase-like.
IPR012265. Ptpn1/Ptpn2.
IPR000387. Tyr/Dual-sp_Pase.
IPR016130. Tyr_Pase_AS.
IPR000242. Tyr_Pase_rcpt/non-rcpt.
[Graphical view ]
Pfami PF00102. Y_phosphatase. 1 hit.
[Graphical view ]
PIRSFi PIRSF000926. Tyr-Ptase_nr1. 1 hit.
PRINTSi PR00700. PRTYPHPHTASE.
SMARTi SM00194. PTPc. 1 hit.
[Graphical view ]
SUPFAMi SSF52799. SSF52799. 1 hit.
PROSITEi PS00383. TYR_PHOSPHATASE_1. 1 hit.
PS50056. TYR_PHOSPHATASE_2. 1 hit.
PS50055. TYR_PHOSPHATASE_PTP. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Identification of novel protein tyrosine phosphatases of hematopoietic cells by polymerase chain reaction amplification."
    Yi T., Cleveland J.L., Ihle J.N.
    Blood 78:2222-2228(1991) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
  2. "The cDNA cloning, nucleotide sequence and expression of an intracellular protein tyrosine phosphatase from mouse testis."
    Miyasaka H., Li S.S.L.
    Biochem. Biophys. Res. Commun. 185:818-825(1992) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Tissue: Testis.
  3. Liao K., Lane M.D.
    Submitted (APR-1995) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
  4. Park K., Byun S.
    Submitted (MAY-1996) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Tissue: Brain.
  5. "A novel receptor-type protein tyrosine phosphatase with a single catalytic domain is specifically expressed in mouse brain."
    Hendriks W., Schepens J., Brugman C., Zeeuwen P., Wieringa B.
    Biochem. J. 305:499-504(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 102-213.
    Strain: BALB/c.
    Tissue: Brain.
  6. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
  7. Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Liver.

Entry informationi

Entry nameiPTN1_MOUSE
AccessioniPrimary (citable) accession number: P35821
Secondary accession number(s): Q60840
, Q62131, Q64498, Q99JS1
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 1, 1994
Last sequence update: November 1, 1997
Last modified: September 3, 2014
This is version 150 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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