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P35821

- PTN1_MOUSE

UniProt

P35821 - PTN1_MOUSE

Protein

Tyrosine-protein phosphatase non-receptor type 1

Gene

Ptpn1

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 151 (01 Oct 2014)
      Sequence version 2 (01 Nov 1997)
      Previous versions | rss
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    Functioni

    Tyrosine-protein phosphatase which acts as a regulator of endoplasmic reticulum unfolded protein response. Mediates dephosphorylation of EIF2AK3/PERK; inactivating the protein kinase activity of EIF2AK3/PERK. May play an important role in CKII- and p60c-src-induced signal transduction cascades. May regulate the EFNA5-EPHA3 signaling pathway which modulates cell reorganization and cell-cell repulsion. May also regulate the hepatocyte growth factor receptor signaling pathway through dephosphorylation of MET By similarity.By similarity

    Catalytic activityi

    Protein tyrosine phosphate + H2O = protein tyrosine + phosphate.PROSITE-ProRule annotation

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei181 – 1811SubstrateBy similarity
    Active sitei215 – 2151Phosphocysteine intermediatePROSITE-ProRule annotation
    Binding sitei262 – 2621SubstrateBy similarity

    GO - Molecular functioni

    1. protein tyrosine phosphatase activity Source: UniProtKB
    2. zinc ion binding Source: Ensembl

    GO - Biological processi

    1. actin cytoskeleton reorganization Source: UniProtKB
    2. endoplasmic reticulum unfolded protein response Source: UniProtKB
    3. insulin receptor signaling pathway Source: MGI
    4. negative regulation of ERK1 and ERK2 cascade Source: BHF-UCL
    5. negative regulation of vascular endothelial growth factor receptor signaling pathway Source: BHF-UCL
    6. peptidyl-tyrosine dephosphorylation Source: UniProtKB
    7. peptidyl-tyrosine dephosphorylation involved in inactivation of protein kinase activity Source: BHF-UCL
    8. platelet-derived growth factor receptor-beta signaling pathway Source: UniProtKB
    9. protein dephosphorylation Source: MGI
    10. regulation of endocytosis Source: UniProtKB
    11. regulation of hepatocyte growth factor receptor signaling pathway Source: Ensembl
    12. regulation of insulin receptor signaling pathway Source: Ensembl
    13. regulation of signal transduction Source: UniProtKB

    Keywords - Molecular functioni

    Hydrolase, Protein phosphatase

    Enzyme and pathway databases

    ReactomeiREACT_198614. Growth hormone receptor signaling.
    REACT_198627. Regulation of IFNA signaling.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Tyrosine-protein phosphatase non-receptor type 1 (EC:3.1.3.48)
    Alternative name(s):
    Protein-tyrosine phosphatase 1B
    Short name:
    PTP-1B
    Protein-tyrosine phosphatase HA2
    Short name:
    PTP-HA2
    Gene namesi
    Name:Ptpn1
    OrganismiMus musculus (Mouse)
    Taxonomic identifieri10090 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
    ProteomesiUP000000589: Chromosome 2

    Organism-specific databases

    MGIiMGI:97805. Ptpn1.

    Subcellular locationi

    Endoplasmic reticulum membrane By similarity; Peripheral membrane protein By similarity; Cytoplasmic side By similarity
    Note: Interacts with EPHA3 at the cell membrane.By similarity

    GO - Cellular componenti

    1. cytoplasmic vesicle Source: Ensembl
    2. cytosol Source: Reactome
    3. early endosome Source: UniProtKB
    4. endoplasmic reticulum membrane Source: UniProtKB-SubCell
    5. plasma membrane Source: Ensembl
    6. sorting endosome Source: BHF-UCL

    Keywords - Cellular componenti

    Endoplasmic reticulum, Membrane

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 432432Tyrosine-protein phosphatase non-receptor type 1PRO_0000094749Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei1 – 11N-acetylmethionineBy similarity
    Modified residuei20 – 201PhosphotyrosineBy similarity
    Modified residuei50 – 501Phosphoserine; by CLK1, CLK2 and PKB/AKT1 or PKB/AKT2By similarity
    Modified residuei66 – 661Phosphotyrosine; by EGFRBy similarity
    Modified residuei215 – 2151Cysteine persulfideBy similarity
    Modified residuei215 – 2151S-nitrosocysteine; in reversibly inhibited formBy similarity
    Modified residuei242 – 2421Phosphoserine; by CLK1 and CLK2By similarity
    Modified residuei243 – 2431Phosphoserine; by CLK1 and CLK2By similarity

    Post-translational modificationi

    Ser-50 is the major site of phosphorylation as compared to Ser-242 and Ser-243. Activated by phosphorylation at Ser-50 By similarity.By similarity
    S-nitrosylation of Cys-215 inactivates the enzyme activity.By similarity
    Sulfhydration at Cys-215 following endoplasmic reticulum stress inactivates the enzyme activity, promoting EIF2AK3/PERK activity.By similarity

    Keywords - PTMi

    Acetylation, Phosphoprotein, S-nitrosylation

    Proteomic databases

    MaxQBiP35821.
    PaxDbiP35821.
    PRIDEiP35821.

    PTM databases

    PhosphoSiteiP35821.

    Expressioni

    Tissue specificityi

    Most abundant in testis. Also found in kidney, spleen, muscle, liver, heart and brain.

    Gene expression databases

    ArrayExpressiP35821.
    BgeeiP35821.
    CleanExiMM_PTPN1.
    GenevestigatoriP35821.

    Interactioni

    Subunit structurei

    Interacts with EPHA3 (phosphorylated); dephosphorylates EPHA3 and may regulate its trafficking and function. Interacts with MET.By similarity

    Protein-protein interaction databases

    BioGridi202476. 1 interaction.
    IntActiP35821. 2 interactions.
    MINTiMINT-4996685.

    Structurei

    3D structure databases

    ProteinModelPortaliP35821.
    SMRiP35821. Positions 1-282.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini3 – 277275Tyrosine-protein phosphatasePROSITE-ProRule annotationAdd
    BLAST

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni215 – 2217Substrate bindingBy similarity

    Sequence similaritiesi

    Contains 1 tyrosine-protein phosphatase domain.PROSITE-ProRule annotation

    Phylogenomic databases

    eggNOGiCOG5599.
    HOGENOMiHOG000273908.
    HOVERGENiHBG008321.
    InParanoidiP35821.
    KOiK05696.
    OMAiMCMATVL.
    OrthoDBiEOG7RV9G4.
    PhylomeDBiP35821.
    TreeFamiTF315897.

    Family and domain databases

    Gene3Di3.90.190.10. 1 hit.
    InterProiIPR029021. Prot-tyrosine_phosphatase-like.
    IPR012265. Ptpn1/Ptpn2.
    IPR000387. Tyr/Dual-sp_Pase.
    IPR016130. Tyr_Pase_AS.
    IPR000242. Tyr_Pase_rcpt/non-rcpt.
    [Graphical view]
    PfamiPF00102. Y_phosphatase. 1 hit.
    [Graphical view]
    PIRSFiPIRSF000926. Tyr-Ptase_nr1. 1 hit.
    PRINTSiPR00700. PRTYPHPHTASE.
    SMARTiSM00194. PTPc. 1 hit.
    [Graphical view]
    SUPFAMiSSF52799. SSF52799. 1 hit.
    PROSITEiPS00383. TYR_PHOSPHATASE_1. 1 hit.
    PS50056. TYR_PHOSPHATASE_2. 1 hit.
    PS50055. TYR_PHOSPHATASE_PTP. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P35821-1 [UniParc]FASTAAdd to Basket

    « Hide

    MEMEKEFEEI DKAGNWAAIY QDIRHEASDF PCKVAKLPKN KNRNRYRDVS    50
    PFDHSRIKLH QEDNDYINAS LIKMEEAQRS YILTQGPLPN TCGHFWEMVW 100
    EQKSRGVVML NRIMEKGSLK CAQYWPQQEE KEMVFDDTGL KLTLISEDVK 150
    SYYTVRQLEL ENLTTKETRE ILHFHYTTWP DFGVPESPAS FLNFLFKVRE 200
    SGSLSLEHGP IVVHCSAGIG RSGTFCLADT CLLLMDKRKD PSSVDIKKVL 250
    LEMRRFRMGL IQTADQLRFS YLAVIEGAKF IMGDSSVQDQ WKELSREDLD 300
    LPPEHVPPPP RPPKRTLEPH NGKCKELFSS HQWVSEETCG DEDSLAREEG 350
    RAQSSAMHSV SSMSPDTEVR RRMVGGGLQS AQASVPTEEE LSSTEEEHKA 400
    HWPSHWKPFL VNVCMATLLA TGAYLCYRVC FH 432
    Length:432
    Mass (Da):49,593
    Last modified:November 1, 1997 - v2
    Checksum:i4843D2DD4C288C48
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti48 – 481D → Y in M97590. (PubMed:1378268)Curated
    Sequence conflicti173 – 1731H → P in AAA64615. 1 PublicationCurated
    Sequence conflicti266 – 2672QL → HV in AAA64615. 1 PublicationCurated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M97590 mRNA. No translation available.
    L40595 mRNA. Translation: AAA64615.1.
    U24700 mRNA. Translation: AAA98605.1.
    Z23057 mRNA. Translation: CAA80592.1.
    BC005729 mRNA. Translation: AAH05729.1.
    BC010191 mRNA. Translation: AAH10191.1.
    CCDSiCCDS17107.1.
    PIRiJN0317.
    RefSeqiNP_035331.3. NM_011201.3.
    UniGeneiMm.277916.

    Genome annotation databases

    EnsembliENSMUST00000029053; ENSMUSP00000029053; ENSMUSG00000027540.
    GeneIDi19246.
    KEGGimmu:19246.
    UCSCiuc008oaj.1. mouse.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M97590 mRNA. No translation available.
    L40595 mRNA. Translation: AAA64615.1 .
    U24700 mRNA. Translation: AAA98605.1 .
    Z23057 mRNA. Translation: CAA80592.1 .
    BC005729 mRNA. Translation: AAH05729.1 .
    BC010191 mRNA. Translation: AAH10191.1 .
    CCDSi CCDS17107.1.
    PIRi JN0317.
    RefSeqi NP_035331.3. NM_011201.3.
    UniGenei Mm.277916.

    3D structure databases

    ProteinModelPortali P35821.
    SMRi P35821. Positions 1-282.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 202476. 1 interaction.
    IntActi P35821. 2 interactions.
    MINTi MINT-4996685.

    Chemistry

    ChEMBLi CHEMBL3336.

    PTM databases

    PhosphoSitei P35821.

    Proteomic databases

    MaxQBi P35821.
    PaxDbi P35821.
    PRIDEi P35821.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSMUST00000029053 ; ENSMUSP00000029053 ; ENSMUSG00000027540 .
    GeneIDi 19246.
    KEGGi mmu:19246.
    UCSCi uc008oaj.1. mouse.

    Organism-specific databases

    CTDi 5770.
    MGIi MGI:97805. Ptpn1.

    Phylogenomic databases

    eggNOGi COG5599.
    HOGENOMi HOG000273908.
    HOVERGENi HBG008321.
    InParanoidi P35821.
    KOi K05696.
    OMAi MCMATVL.
    OrthoDBi EOG7RV9G4.
    PhylomeDBi P35821.
    TreeFami TF315897.

    Enzyme and pathway databases

    Reactomei REACT_198614. Growth hormone receptor signaling.
    REACT_198627. Regulation of IFNA signaling.

    Miscellaneous databases

    ChiTaRSi PTPN1. mouse.
    NextBioi 296070.
    PROi P35821.
    SOURCEi Search...

    Gene expression databases

    ArrayExpressi P35821.
    Bgeei P35821.
    CleanExi MM_PTPN1.
    Genevestigatori P35821.

    Family and domain databases

    Gene3Di 3.90.190.10. 1 hit.
    InterProi IPR029021. Prot-tyrosine_phosphatase-like.
    IPR012265. Ptpn1/Ptpn2.
    IPR000387. Tyr/Dual-sp_Pase.
    IPR016130. Tyr_Pase_AS.
    IPR000242. Tyr_Pase_rcpt/non-rcpt.
    [Graphical view ]
    Pfami PF00102. Y_phosphatase. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF000926. Tyr-Ptase_nr1. 1 hit.
    PRINTSi PR00700. PRTYPHPHTASE.
    SMARTi SM00194. PTPc. 1 hit.
    [Graphical view ]
    SUPFAMi SSF52799. SSF52799. 1 hit.
    PROSITEi PS00383. TYR_PHOSPHATASE_1. 1 hit.
    PS50056. TYR_PHOSPHATASE_2. 1 hit.
    PS50055. TYR_PHOSPHATASE_PTP. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Identification of novel protein tyrosine phosphatases of hematopoietic cells by polymerase chain reaction amplification."
      Yi T., Cleveland J.L., Ihle J.N.
      Blood 78:2222-2228(1991) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    2. "The cDNA cloning, nucleotide sequence and expression of an intracellular protein tyrosine phosphatase from mouse testis."
      Miyasaka H., Li S.S.L.
      Biochem. Biophys. Res. Commun. 185:818-825(1992) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
      Tissue: Testis.
    3. Liao K., Lane M.D.
      Submitted (APR-1995) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    4. Park K., Byun S.
      Submitted (MAY-1996) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
      Tissue: Brain.
    5. "A novel receptor-type protein tyrosine phosphatase with a single catalytic domain is specifically expressed in mouse brain."
      Hendriks W., Schepens J., Brugman C., Zeeuwen P., Wieringa B.
      Biochem. J. 305:499-504(1995) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 102-213.
      Strain: BALB/c.
      Tissue: Brain.
    6. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    7. Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
      Tissue: Liver.

    Entry informationi

    Entry nameiPTN1_MOUSE
    AccessioniPrimary (citable) accession number: P35821
    Secondary accession number(s): Q60840
    , Q62131, Q64498, Q99JS1
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: June 1, 1994
    Last sequence update: November 1, 1997
    Last modified: October 1, 2014
    This is version 151 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. MGD cross-references
      Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3