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Protein

Polycomb group protein Psc

Gene

Psc

Organism
Drosophila melanogaster (Fruit fly)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Polycomb group (PcG) protein. PcG proteins act by forming multiprotein complexes, which are required to maintain the transcriptionally repressive state of homeotic genes throughout development. PcG proteins are not required to initiate repression, but to maintain it during later stages of development. Component of the PcG multiprotein PRC1 complex, a complex that acts via chromatin remodeling and modification of histones; it mediates monoubiquitination of histone H2A 'Lys-118', rendering chromatin heritably changed in its expressibility. Needed to maintain expression patterns of the homeotic selector genes of the Antennapedia (Antp-C) and Bithorax (BX-C) complexes, and hence for the maintenance of segmental determination.1 Publication

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Zinc fingeri263 – 30240RING-typePROSITE-ProRule annotationAdd
BLAST

GO - Molecular functioni

  • core promoter binding Source: FlyBase
  • DNA binding Source: FlyBase
  • zinc ion binding Source: FlyBase

GO - Biological processi

  • axon guidance Source: FlyBase
  • chromatin remodeling Source: FlyBase
  • chromatin silencing Source: FlyBase
  • syncytial blastoderm mitotic cell cycle Source: FlyBase
  • ventral cord development Source: FlyBase
Complete GO annotation...

Keywords - Molecular functioni

Developmental protein

Keywords - Ligandi

DNA-binding, Metal-binding, Zinc

Enzyme and pathway databases

ReactomeiR-DME-3108214. SUMOylation of DNA damage response and repair proteins.
R-DME-4570464. SUMOylation of RNA binding proteins.

Names & Taxonomyi

Protein namesi
Recommended name:
Polycomb group protein Psc
Alternative name(s):
Protein posterior sex combs
Gene namesi
Name:Psc
ORF Names:CG3886
OrganismiDrosophila melanogaster (Fruit fly)
Taxonomic identifieri7227 [NCBI]
Taxonomic lineageiEukaryotaMetazoaEcdysozoaArthropodaHexapodaInsectaPterygotaNeopteraEndopterygotaDipteraBrachyceraMuscomorphaEphydroideaDrosophilidaeDrosophilaSophophora
Proteomesi
  • UP000000803 Componenti: Chromosome 2R

Organism-specific databases

FlyBaseiFBgn0005624. Psc.

Subcellular locationi

GO - Cellular componenti

  • intercalary heterochromatin Source: FlyBase
  • nucleus Source: FlyBase
  • PcG protein complex Source: FlyBase
  • polytene chromosome band Source: FlyBase
  • PRC1 complex Source: FlyBase
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 16011601Polycomb group protein PscPRO_0000056388Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei656 – 6561Phosphoserine1 Publication
Modified residuei658 – 6581Phosphoserine1 Publication
Modified residuei1139 – 11391Phosphoserine1 Publication
Modified residuei1222 – 12221Phosphothreonine1 Publication
Modified residuei1236 – 12361Phosphothreonine1 Publication
Modified residuei1251 – 12511Phosphothreonine1 Publication
Modified residuei1253 – 12531Phosphoserine1 Publication
Modified residuei1266 – 12661Phosphoserine1 Publication
Modified residuei1274 – 12741Phosphoserine1 Publication

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiP35820.
PRIDEiP35820.

PTM databases

iPTMnetiP35820.

Expressioni

Gene expression databases

BgeeiP35820.
ExpressionAtlasiP35820. differential.
GenevisibleiP35820. DM.

Interactioni

Subunit structurei

Component of PRC1 complex, which contains many PcG proteins like Pc, ph, Scm, Psc, Sce and also chromatin-remodeling proteins such as histone deacetylases. This complex is distinct from the Esc/E(z) complex, at least composed of esc, E(z), Su(z)12, Rpd3 and Caf1. The 2 complexes however cooperate and interact together during the first 3 hours of development to establish PcG silencing.2 Publications

Protein-protein interaction databases

BioGridi62201. 14 interactions.
IntActiP35820. 7 interactions.
STRINGi7227.FBpp0086911.

Structurei

3D structure databases

ProteinModelPortaliP35820.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi15 – 155141Thr-richAdd
BLAST
Compositional biasi178 – 21235Ser-richAdd
BLAST
Compositional biasi565 – 65894Ser-richAdd
BLAST
Compositional biasi806 – 87873Pro-richAdd
BLAST
Compositional biasi1022 – 107958Gly-richAdd
BLAST
Compositional biasi1170 – 1406237Pro-richAdd
BLAST

Sequence similaritiesi

Contains 1 RING-type zinc finger.PROSITE-ProRule annotation

Zinc finger

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Zinc fingeri263 – 30240RING-typePROSITE-ProRule annotationAdd
BLAST

Keywords - Domaini

Zinc-finger

Phylogenomic databases

eggNOGiKOG2660. Eukaryota.
ENOG410XPCN. LUCA.
GeneTreeiENSGT00550000074463.
InParanoidiP35820.
KOiK11459.
OMAiSIDIMYK.
OrthoDBiEOG77T14W.
PhylomeDBiP35820.

Family and domain databases

Gene3Di3.30.40.10. 1 hit.
InterProiIPR032443. RAWUL.
IPR001841. Znf_RING.
IPR013083. Znf_RING/FYVE/PHD.
IPR017907. Znf_RING_CS.
[Graphical view]
PfamiPF16207. RAWUL. 1 hit.
[Graphical view]
PROSITEiPS00518. ZF_RING_1. 1 hit.
PS50089. ZF_RING_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P35820-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MMTPESKAIQ PAAATTKQTA EATATTTMAH TQQKSQLSTL AKTTTTTATN
60 70 80 90 100
KAAKSVVSNA NSSGNNSSKK LALSQSQKTT TTTTPPTTTT TTTAAAAAEA
110 120 130 140 150
TTNADKMQKQ QQLKQQLFAA CSIKVKSENT LATTANAALA AATTTTTTAT
160 170 180 190 200
PALATGKAAK TILENGIKKE STPPAVESVE ASSSSSSSSS SSSSSSSSWP
210 220 230 240 250
TTRRATSEDA SSNGGASADE EKSEEDPTAA VAASSTATTT SDLATTSRPR
260 270 280 290 300
PVLLTAVNPH IICHLCQGYL INATTIVECL HSFCHSCLIN HLRKERFCPR
310 320 330 340 350
CEMVINNAKP NIKSDTTLQA IVYKLVPGLY ERELMRKRAF YKDRPEEAAL
360 370 380 390 400
ATPEQRGDDT EHLIFSPSDD MSLSLEYAEL GELKTDSEPE LVDTLRPRYL
410 420 430 440 450
QCPAMCRVSH LKKFVYDKFE IDAQRFSIDI MYKVKTIVLL DYYTLMDIAY
460 470 480 490 500
IYTWKRDAPM RFYYRVYESP QPLVKPAPRR VLPLKLEKQE RENQEQQLAV
510 520 530 540 550
EVASSKVEPV SLPEDQKAEA SIKVEEQEST REIVKEVIKD VAATPPTETL
560 570 580 590 600
KLVINRNMLD KREKSHSPQM SSKSSSKSSP CTPVSSPSEP NIKLKIDLSK
610 620 630 640 650
QNSVTIIDMS DPERREIVKP LKPEKESRSK KKDKDGSPKS SSSSSSSSSG
660 670 680 690 700
ERKRKSPSPL TVPPLTIRTE RIMSPSGVST LSPRVTSGAF SEDPKSEFLK
710 720 730 740 750
SFALKPIKVK VESPERTLNN RAITPPSPSV QQSASPKSKG NNLDDSILMK
760 770 780 790 800
PPSCMPPKSI ASSKRKSKEP VKAVSKKQKL SPPLPTVDFK IRLPVTNGNS
810 820 830 840 850
SGTASPKIEK PLMPPPAKPP MLAPRKLQPS AQFAPPPSPI HHHAGVQMSA
860 870 880 890 900
PGNRTPIAKR YQPILPKASR PNPFANIPND VNRLLKDAGT EIKSIGGGSV
910 920 930 940 950
ENNSNAAQKP HLYGPKGESK MGPPALPATT PSQGNKNVGK QAGNLPMSAP
960 970 980 990 1000
PNKGNSSNNY LNLALFNSSK CKGKEAPPGC RTPMYTPNSP IYSPSSPQYV
1010 1020 1030 1040 1050
PSYNIPTMPT YKYTPKPTPN SGSGNGGSGS YLQNMLGGGN GGSLGGLFPS
1060 1070 1080 1090 1100
PPTKSDQNTN PAQGGGGSSS ATQSGGNNGI VNNNIYMPNE DAPEKQQVKV
1110 1120 1130 1140 1150
KSLLNSCNIN IPSSLSITIS RDNGDSSSPN NGQHPKHKSP VNNYIEIVKL
1160 1170 1180 1190 1200
PDQPQDQVQA AKEAQKRQSP PAAVPGHLAA KLPPPPPSKA IPSPQHLVSR
1210 1220 1230 1240 1250
MTPPQLPKVA TPPPPSSPRV ITPPKTSPPA NAAKVTPLKP VLTPTQVDKK
1260 1270 1280 1290 1300
TPSPEKRTAA QMGSHSPTAS ENKSPKGGPA GVANSTGGAQ NGDPAAKKFR
1310 1320 1330 1340 1350
PILPRQNGMP ELAPKLPTLA PFVGFNPLQN PAAGKKVPPS KKSPNAGAAA
1360 1370 1380 1390 1400
HQSGQQKLVN GGQSQPAQQK TSPPAQKNQQ QVKKVSKNPT PPPPSLPAVG
1410 1420 1430 1440 1450
KMMPHPVMHS QNAPLSIASS ASAAAVASGQ LDLSNFLKEN LRRVHAAQAA
1460 1470 1480 1490 1500
QAAQVAAAAN QSNMMYNLAQ MGHMTPAMYN YQQAYFREQL SRMQRVGNEV
1510 1520 1530 1540 1550
FNDYLQKLKT AAATGGGGPV EGELKPMLPT VTLPSPGATP PAASPKTSPL
1560 1570 1580 1590 1600
PAGKLTAAAT APQTKGNSSS GAANARQQTA ATGNNGATVP AASLPPATKS

K
Length:1,601
Mass (Da):169,833
Last modified:September 27, 2005 - v2
Checksum:i7C6264A12CDF3D49
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti23 – 231T → TPEAT (PubMed:1833647).Curated
Sequence conflicti94 – 963AAA → T in CAA41965 (PubMed:1833647).Curated
Sequence conflicti130 – 1301T → A in CAA41965 (PubMed:1833647).Curated
Sequence conflicti136 – 1361N → T in CAA41965 (PubMed:1833647).Curated
Sequence conflicti200 – 2001P → S in CAA41965 (PubMed:1833647).Curated
Sequence conflicti451 – 4511I → T in CAA41965 (PubMed:1833647).Curated
Sequence conflicti513 – 5131P → A in CAA41965 (PubMed:1833647).Curated
Sequence conflicti526 – 5272EQ → GE in CAA41965 (PubMed:1833647).Curated
Sequence conflicti570 – 5701M → L in CAA41965 (PubMed:1833647).Curated
Sequence conflicti906 – 9061A → S in CAA41965 (PubMed:1833647).Curated
Sequence conflicti919 – 9191S → T in CAA41965 (PubMed:1833647).Curated
Sequence conflicti969 – 9691S → N in CAA41965 (PubMed:1833647).Curated
Sequence conflicti1279 – 12791P → A in CAA41965 (PubMed:1833647).Curated
Sequence conflicti1289 – 12891A → T in CAA41965 (PubMed:1833647).Curated
Sequence conflicti1364 – 13663SQP → PQS in CAA41965 (PubMed:1833647).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X59275 mRNA. Translation: CAA41965.1.
DQ022542 mRNA. Translation: AAZ20646.1.
AE013599 Genomic DNA. Translation: AAF58434.1.
PIRiS17983.
RefSeqiNP_001286368.1. NM_001299439.1.
NP_001286369.1. NM_001299440.1.
NP_523725.2. NM_079001.3.
UniGeneiDm.2703.

Genome annotation databases

EnsemblMetazoaiFBtr0087798; FBpp0086911; FBgn0005624.
FBtr0345196; FBpp0311395; FBgn0005624.
FBtr0345375; FBpp0311526; FBgn0005624.
GeneIDi36431.
KEGGidme:Dmel_CG3886.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X59275 mRNA. Translation: CAA41965.1.
DQ022542 mRNA. Translation: AAZ20646.1.
AE013599 Genomic DNA. Translation: AAF58434.1.
PIRiS17983.
RefSeqiNP_001286368.1. NM_001299439.1.
NP_001286369.1. NM_001299440.1.
NP_523725.2. NM_079001.3.
UniGeneiDm.2703.

3D structure databases

ProteinModelPortaliP35820.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi62201. 14 interactions.
IntActiP35820. 7 interactions.
STRINGi7227.FBpp0086911.

PTM databases

iPTMnetiP35820.

Proteomic databases

PaxDbiP35820.
PRIDEiP35820.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblMetazoaiFBtr0087798; FBpp0086911; FBgn0005624.
FBtr0345196; FBpp0311395; FBgn0005624.
FBtr0345375; FBpp0311526; FBgn0005624.
GeneIDi36431.
KEGGidme:Dmel_CG3886.

Organism-specific databases

CTDi100653366.
FlyBaseiFBgn0005624. Psc.

Phylogenomic databases

eggNOGiKOG2660. Eukaryota.
ENOG410XPCN. LUCA.
GeneTreeiENSGT00550000074463.
InParanoidiP35820.
KOiK11459.
OMAiSIDIMYK.
OrthoDBiEOG77T14W.
PhylomeDBiP35820.

Enzyme and pathway databases

ReactomeiR-DME-3108214. SUMOylation of DNA damage response and repair proteins.
R-DME-4570464. SUMOylation of RNA binding proteins.

Miscellaneous databases

ChiTaRSiPsc. fly.
GenomeRNAii36431.
PROiP35820.

Gene expression databases

BgeeiP35820.
ExpressionAtlasiP35820. differential.
GenevisibleiP35820. DM.

Family and domain databases

Gene3Di3.30.40.10. 1 hit.
InterProiIPR032443. RAWUL.
IPR001841. Znf_RING.
IPR013083. Znf_RING/FYVE/PHD.
IPR017907. Znf_RING_CS.
[Graphical view]
PfamiPF16207. RAWUL. 1 hit.
[Graphical view]
PROSITEiPS00518. ZF_RING_1. 1 hit.
PS50089. ZF_RING_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Drosophila genes Posterior Sex Combs and Suppressor two of zeste encode proteins with homology to the murine bmi-1 oncogene."
    Brunk B.P., Martin E.C., Sharp E., Adler P.N.
    Nature 353:351-353(1991) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION.
  2. "Analysis of a polycomb group protein defines regions that link repressive activity on nucleosomal templates to in vivo function."
    King I.F.G., Emmons R.B., Francis N.J., Wild B., Mueller J., Kingston R.E., Wu C.-T.
    Mol. Cell. Biol. 25:6578-6591(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Strain: Psce25.
  3. "The genome sequence of Drosophila melanogaster."
    Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D., Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F., George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N., Sutton G.G., Wortman J.R., Yandell M.D.
    , Zhang Q., Chen L.X., Brandon R.C., Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C., Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A., An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A., Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V., Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J., Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E., Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B., Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I., Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C., Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S., Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M., Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M., Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D., Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F., Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D., Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A., Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C., McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C., Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L., Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R., Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V., Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F., Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J., Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R., Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y., Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T., Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S., Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W., Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M., Venter J.C.
    Science 287:2185-2195(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: Berkeley.
  4. Cited for: GENOME REANNOTATION.
    Strain: Berkeley.
  5. "A Drosophila Polycomb group complex includes Zeste and dTAFII proteins."
    Saurin A.J., Shao Z., Erdjument-Bromage H., Tempst P., Kingston R.E.
    Nature 412:655-660(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION IN THE PRC1 COMPLEX WITH SCE; PC AND PH.
  6. "Reconstitution of a functional core polycomb repressive complex."
    Francis N.J., Saurin A.J., Shao Z., Kingston R.E.
    Mol. Cell 8:545-556(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION IN A PCG COMPLEX WITH SCE; PC AND PH.
  7. "Phosphoproteome analysis of Drosophila melanogaster embryos."
    Zhai B., Villen J., Beausoleil S.A., Mintseris J., Gygi S.P.
    J. Proteome Res. 7:1675-1682(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-656; SER-658; SER-1139; THR-1222; THR-1236; THR-1251; SER-1253; SER-1266 AND SER-1274, IDENTIFICATION BY MASS SPECTROMETRY.
    Tissue: Embryo.

Entry informationi

Entry nameiPSC_DROME
AccessioniPrimary (citable) accession number: P35820
Secondary accession number(s): Q9V6I9
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 1, 1994
Last sequence update: September 27, 2005
Last modified: June 8, 2016
This is version 115 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programDrosophila annotation project

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Drosophila
    Drosophila: entries, gene names and cross-references to FlyBase
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.