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Protein

Bromodomain-containing factor 1

Gene

BDF1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Transcription factor involved in the expression of a broad class of genes including snRNAs. Required for sporulation and DNA-damage repair. Prevents the spreading of SIR silencing at telomeres and protects histone H4, but not H3, from deacetylation.8 Publications

Miscellaneous

Present with 8100 molecules/cell in log phase SD medium.1 Publication

GO - Molecular functioni

  • chromatin binding Source: SGD
  • core promoter binding Source: SGD
  • lysine-acetylated histone binding Source: SGD
  • TFIID-class transcription factor binding Source: SGD

GO - Biological processi

  • chromatin remodeling Source: SGD
  • DNA repair Source: SGD
  • negative regulation of heterochromatin assembly Source: SGD
  • positive regulation of histone exchange Source: SGD
  • regulation of chromatin silencing at silent mating-type cassette Source: SGD
  • regulation of chromatin silencing at telomere Source: SGD
  • snRNA transcription Source: SGD
  • sporulation resulting in formation of a cellular spore Source: UniProtKB-KW

Keywordsi

Biological processDNA damage, DNA repair, Sporulation, Transcription, Transcription regulation

Enzyme and pathway databases

BioCyciYEAST:G3O-32463-MONOMER.
ReactomeiR-SCE-3371568. Attenuation phase.
R-SCE-5689901. Metalloprotease DUBs.
R-SCE-6781823. Formation of TC-NER Pre-Incision Complex.
R-SCE-6782135. Dual incision in TC-NER.
R-SCE-6782210. Gap-filling DNA repair synthesis and ligation in TC-NER.

Names & Taxonomyi

Protein namesi
Recommended name:
Bromodomain-containing factor 1
Gene namesi
Name:BDF1
Ordered Locus Names:YLR399C
ORF Names:L8084.18
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome XII

Organism-specific databases

EuPathDBiFungiDB:YLR399C.
SGDiS000004391. BDF1.

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Nucleus

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi187Y → F: Impairs interaction with histones H3 and H4; when associated with F-354. 1 Publication1
Mutagenesisi354Y → F: Impairs interaction with histones H3 and H4; when associated with F-187. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002111761 – 686Bromodomain-containing factor 1Add BLAST686

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei270PhosphoserineCombined sources1
Modified residuei429PhosphoserineCombined sources1
Modified residuei615PhosphoserineCombined sources1
Modified residuei659PhosphoserineCombined sources1

Post-translational modificationi

Phosphorylated by the casein kinase CK2 complex.1 Publication

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiP35817.
PRIDEiP35817.

PTM databases

iPTMnetiP35817.

Interactioni

Subunit structurei

Interacts with the TFIID subunit TAF7 and with acetylated histones H3 and H4.4 Publications

Binary interactionsi

Show more details

GO - Molecular functioni

  • lysine-acetylated histone binding Source: SGD
  • TFIID-class transcription factor binding Source: SGD

Protein-protein interaction databases

BioGridi31658. 332 interactors.
DIPiDIP-1624N.
IntActiP35817. 30 interactors.
MINTiMINT-407031.
STRINGi4932.YLR399C.

Structurei

3D structure databases

ProteinModelPortaliP35817.
SMRiP35817.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini165 – 237Bromo 1PROSITE-ProRule annotationAdd BLAST73
Domaini332 – 404Bromo 2PROSITE-ProRule annotationAdd BLAST73
Domaini518 – 598NETPROSITE-ProRule annotationAdd BLAST81

Coiled coil

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Coiled coili460 – 499Sequence analysisAdd BLAST40

Keywords - Domaini

Bromodomain, Coiled coil, Repeat

Phylogenomic databases

GeneTreeiENSGT00760000119206.
HOGENOMiHOG000248774.
InParanoidiP35817.
KOiK11684.
OMAiCDSILRE.
OrthoDBiEOG092C3V5H.

Family and domain databases

Gene3Di1.20.920.10. 2 hits.
InterProiView protein in InterPro
IPR001487. Bromodomain.
IPR036427. Bromodomain-like_sf.
IPR018359. Bromodomain_CS.
IPR027353. NET_dom.
PfamiView protein in Pfam
PF17035. BET. 1 hit.
PF00439. Bromodomain. 2 hits.
PRINTSiPR00503. BROMODOMAIN.
SMARTiView protein in SMART
SM00297. BROMO. 2 hits.
SUPFAMiSSF47370. SSF47370. 2 hits.
PROSITEiView protein in PROSITE
PS00633. BROMODOMAIN_1. 2 hits.
PS50014. BROMODOMAIN_2. 2 hits.
PS51525. NET. 1 hit.

Sequencei

Sequence statusi: Complete.

P35817-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTDITPVQND VDVNGNNVND DVSSNLKRPI DQGDPSNGLA EEENPANNQL
60 70 80 90 100
HLKKARLDGD ALTSSPAGLA ENGIEGATLA ANGENGYNAT GSGAEDEQQG
110 120 130 140 150
LKKEEGGQGT KQEDLDENSK QELPMEVPKE PAPAPPPEPD MNNLPQNPIP
160 170 180 190 200
KHQQKHALLA IKAVKRLKDA RPFLQPVDPV KLDIPFYFNY IKRPMDLSTI
210 220 230 240 250
ERKLNVGAYE VPEQITEDFN LMVNNSIKFN GPNAGISQMA RNIQASFEKH
260 270 280 290 300
MLNMPAKDAP PVIAKGRRSS AQEDAPIVIR RAQTHNGRPK RTIHPPKSKD
310 320 330 340 350
IYPYESKKPK SKRLQQAMKF CQSVLKELMA KKHASYNYPF LEPVDPVSMN
360 370 380 390 400
LPTYFDYVKE PMDLGTIAKK LNDWQYQTME DFERDVRLVF KNCYTFNPDG
410 420 430 440 450
TIVNMMGHRL EEVFNSKWAD RPNLDDYDSD EDSRTQGDYD DYESEYSESD
460 470 480 490 500
IDETIITNPA IQYLEEQLAR MKVELQQLKK QELEKIRKER RLARGSKKRG
510 520 530 540 550
KRSKGRSGSK NASSKGRRDK KNKLKTVVTY DMKRIITERI NDLPTSKLER
560 570 580 590 600
AIDIIKKSMP NISEDDEVEL DLDTLDNHTI LTLYNTFFRQ YESSSGASNG
610 620 630 640 650
LDGTSGVTRD ASSLSPTSAG SRKRRSKALS QEEQSRQIEK IKNKLAILDS
660 670 680
ASPLSQNGSP GQIQSAAHNG FSSSSDDDVS SESEEE
Length:686
Mass (Da):76,978
Last modified:July 15, 1999 - v3
Checksum:i8CCD52F41F91D0DA
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti8Q → LC in CAA79377 (PubMed:7816623).Curated1
Sequence conflicti93 – 94GA → R in AAA89115 (PubMed:7791775).Curated2
Sequence conflicti94A → P in CAA79377 (PubMed:7816623).Curated1
Sequence conflicti282A → P in CAA79377 (PubMed:7816623).Curated1
Sequence conflicti385D → E in CAA79377 (PubMed:7816623).Curated1
Sequence conflicti493A → R in AAA35048 (PubMed:8321235).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z18944 Genomic DNA. Translation: CAA79377.1.
U18116 Genomic DNA. Translation: AAA89115.1.
U19729 Genomic DNA. Translation: AAB82357.1.
L13469 mRNA. Translation: AAA35048.1.
BK006945 Genomic DNA. Translation: DAA09700.1.
PIRiS55955.
RefSeqiNP_013503.1. NM_001182287.1.

Genome annotation databases

EnsemblFungiiYLR399C; YLR399C; YLR399C.
GeneIDi851115.
KEGGisce:YLR399C.

Similar proteinsi

Entry informationi

Entry nameiBDF1_YEAST
AccessioniPrimary (citable) accession number: P35817
Secondary accession number(s): D6VZ34, Q06048
Entry historyiIntegrated into UniProtKB/Swiss-Prot: June 1, 1994
Last sequence update: July 15, 1999
Last modified: November 22, 2017
This is version 168 of the entry and version 3 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  2. Yeast chromosome XII
    Yeast (Saccharomyces cerevisiae) chromosome XII: entries and gene names