Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Bromodomain-containing factor 1

Gene

BDF1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Transcription factor involved in the expression of a broad class of genes including snRNAs. Required for sporulation and DNA-damage repair. Prevents the spreading of SIR silencing at telomeres and protects histone H4, but not H3, from deacetylation.8 Publications

GO - Molecular functioni

  • chromatin binding Source: SGD
  • core promoter binding Source: SGD
  • lysine-acetylated histone binding Source: SGD
  • TFIID-class transcription factor binding Source: SGD

GO - Biological processi

  • chromatin remodeling Source: SGD
  • DNA repair Source: SGD
  • negative regulation of heterochromatin assembly Source: SGD
  • positive regulation of histone exchange Source: SGD
  • regulation of chromatin silencing at silent mating-type cassette Source: SGD
  • regulation of chromatin silencing at telomere Source: SGD
  • snRNA transcription Source: SGD
  • sporulation resulting in formation of a cellular spore Source: UniProtKB-KW
Complete GO annotation...

Keywords - Biological processi

DNA damage, DNA repair, Sporulation, Transcription, Transcription regulation

Enzyme and pathway databases

BioCyciYEAST:G3O-32463-MONOMER.
ReactomeiR-SCE-3371568. Attenuation phase.
R-SCE-5689901. Metalloprotease DUBs.
R-SCE-6781823. Formation of TC-NER Pre-Incision Complex.
R-SCE-6782135. Dual incision in TC-NER.
R-SCE-6782210. Gap-filling DNA repair synthesis and ligation in TC-NER.

Names & Taxonomyi

Protein namesi
Recommended name:
Bromodomain-containing factor 1
Gene namesi
Name:BDF1
Ordered Locus Names:YLR399C
ORF Names:L8084.18
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome XII

Organism-specific databases

EuPathDBiFungiDB:YLR399C.
SGDiS000004391. BDF1.

Subcellular locationi

GO - Cellular componenti

  • nuclear chromatin Source: SGD
  • Swr1 complex Source: SGD
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi187Y → F: Impairs interaction with histones H3 and H4; when associated with F-354. 1 Publication1
Mutagenesisi354Y → F: Impairs interaction with histones H3 and H4; when associated with F-187. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002111761 – 686Bromodomain-containing factor 1Add BLAST686

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei270PhosphoserineCombined sources1
Modified residuei429PhosphoserineCombined sources1
Modified residuei615PhosphoserineCombined sources1
Modified residuei659PhosphoserineCombined sources1

Post-translational modificationi

Phosphorylated by the casein kinase CK2 complex.1 Publication

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiP35817.
PRIDEiP35817.

PTM databases

iPTMnetiP35817.

Interactioni

Subunit structurei

Interacts with the TFIID subunit TAF7 and with acetylated histones H3 and H4.4 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
BDF2Q074425EBI-3493,EBI-37620
HHT2P618302EBI-3493,EBI-8098
HTZ1Q126924EBI-3493,EBI-8080
SWR1Q054713EBI-3493,EBI-22102
TAF1P466773EBI-3493,EBI-18855
TAF12Q037612EBI-3493,EBI-35097
TAF6P530402EBI-3493,EBI-18876
TAF7Q050215EBI-3493,EBI-27490
VPS71Q034332EBI-3493,EBI-27814
YAF9P539303EBI-3493,EBI-28841

GO - Molecular functioni

  • lysine-acetylated histone binding Source: SGD
  • TFIID-class transcription factor binding Source: SGD

Protein-protein interaction databases

BioGridi31658. 179 interactors.
DIPiDIP-1624N.
IntActiP35817. 30 interactors.
MINTiMINT-407031.

Structurei

3D structure databases

ProteinModelPortaliP35817.
SMRiP35817.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini165 – 237Bromo 1PROSITE-ProRule annotationAdd BLAST73
Domaini332 – 404Bromo 2PROSITE-ProRule annotationAdd BLAST73
Domaini518 – 598NETPROSITE-ProRule annotationAdd BLAST81

Coiled coil

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Coiled coili460 – 499Sequence analysisAdd BLAST40

Sequence similaritiesi

Contains 2 bromo domains.PROSITE-ProRule annotation
Contains 1 NET domain.PROSITE-ProRule annotation

Keywords - Domaini

Bromodomain, Coiled coil, Repeat

Phylogenomic databases

GeneTreeiENSGT00760000119206.
HOGENOMiHOG000248774.
InParanoidiP35817.
KOiK11684.
OMAiFNYPFLE.
OrthoDBiEOG092C3V5H.

Family and domain databases

Gene3Di1.20.920.10. 2 hits.
InterProiIPR001487. Bromodomain.
IPR018359. Bromodomain_CS.
IPR027353. NET_dom.
[Graphical view]
PfamiPF17035. BET. 1 hit.
PF00439. Bromodomain. 2 hits.
[Graphical view]
PRINTSiPR00503. BROMODOMAIN.
SMARTiSM00297. BROMO. 2 hits.
[Graphical view]
SUPFAMiSSF47370. SSF47370. 2 hits.
PROSITEiPS00633. BROMODOMAIN_1. 2 hits.
PS50014. BROMODOMAIN_2. 2 hits.
PS51525. NET. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P35817-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTDITPVQND VDVNGNNVND DVSSNLKRPI DQGDPSNGLA EEENPANNQL
60 70 80 90 100
HLKKARLDGD ALTSSPAGLA ENGIEGATLA ANGENGYNAT GSGAEDEQQG
110 120 130 140 150
LKKEEGGQGT KQEDLDENSK QELPMEVPKE PAPAPPPEPD MNNLPQNPIP
160 170 180 190 200
KHQQKHALLA IKAVKRLKDA RPFLQPVDPV KLDIPFYFNY IKRPMDLSTI
210 220 230 240 250
ERKLNVGAYE VPEQITEDFN LMVNNSIKFN GPNAGISQMA RNIQASFEKH
260 270 280 290 300
MLNMPAKDAP PVIAKGRRSS AQEDAPIVIR RAQTHNGRPK RTIHPPKSKD
310 320 330 340 350
IYPYESKKPK SKRLQQAMKF CQSVLKELMA KKHASYNYPF LEPVDPVSMN
360 370 380 390 400
LPTYFDYVKE PMDLGTIAKK LNDWQYQTME DFERDVRLVF KNCYTFNPDG
410 420 430 440 450
TIVNMMGHRL EEVFNSKWAD RPNLDDYDSD EDSRTQGDYD DYESEYSESD
460 470 480 490 500
IDETIITNPA IQYLEEQLAR MKVELQQLKK QELEKIRKER RLARGSKKRG
510 520 530 540 550
KRSKGRSGSK NASSKGRRDK KNKLKTVVTY DMKRIITERI NDLPTSKLER
560 570 580 590 600
AIDIIKKSMP NISEDDEVEL DLDTLDNHTI LTLYNTFFRQ YESSSGASNG
610 620 630 640 650
LDGTSGVTRD ASSLSPTSAG SRKRRSKALS QEEQSRQIEK IKNKLAILDS
660 670 680
ASPLSQNGSP GQIQSAAHNG FSSSSDDDVS SESEEE
Length:686
Mass (Da):76,978
Last modified:July 15, 1999 - v3
Checksum:i8CCD52F41F91D0DA
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti8Q → LC in CAA79377 (PubMed:7816623).Curated1
Sequence conflicti93 – 94GA → R in AAA89115 (PubMed:7791775).Curated2
Sequence conflicti94A → P in CAA79377 (PubMed:7816623).Curated1
Sequence conflicti282A → P in CAA79377 (PubMed:7816623).Curated1
Sequence conflicti385D → E in CAA79377 (PubMed:7816623).Curated1
Sequence conflicti493A → R in AAA35048 (PubMed:8321235).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z18944 Genomic DNA. Translation: CAA79377.1.
U18116 Genomic DNA. Translation: AAA89115.1.
U19729 Genomic DNA. Translation: AAB82357.1.
L13469 mRNA. Translation: AAA35048.1.
BK006945 Genomic DNA. Translation: DAA09700.1.
PIRiS55955.
RefSeqiNP_013503.1. NM_001182287.1.

Genome annotation databases

EnsemblFungiiYLR399C; YLR399C; YLR399C.
GeneIDi851115.
KEGGisce:YLR399C.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z18944 Genomic DNA. Translation: CAA79377.1.
U18116 Genomic DNA. Translation: AAA89115.1.
U19729 Genomic DNA. Translation: AAB82357.1.
L13469 mRNA. Translation: AAA35048.1.
BK006945 Genomic DNA. Translation: DAA09700.1.
PIRiS55955.
RefSeqiNP_013503.1. NM_001182287.1.

3D structure databases

ProteinModelPortaliP35817.
SMRiP35817.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi31658. 179 interactors.
DIPiDIP-1624N.
IntActiP35817. 30 interactors.
MINTiMINT-407031.

PTM databases

iPTMnetiP35817.

Proteomic databases

MaxQBiP35817.
PRIDEiP35817.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYLR399C; YLR399C; YLR399C.
GeneIDi851115.
KEGGisce:YLR399C.

Organism-specific databases

EuPathDBiFungiDB:YLR399C.
SGDiS000004391. BDF1.

Phylogenomic databases

GeneTreeiENSGT00760000119206.
HOGENOMiHOG000248774.
InParanoidiP35817.
KOiK11684.
OMAiFNYPFLE.
OrthoDBiEOG092C3V5H.

Enzyme and pathway databases

BioCyciYEAST:G3O-32463-MONOMER.
ReactomeiR-SCE-3371568. Attenuation phase.
R-SCE-5689901. Metalloprotease DUBs.
R-SCE-6781823. Formation of TC-NER Pre-Incision Complex.
R-SCE-6782135. Dual incision in TC-NER.
R-SCE-6782210. Gap-filling DNA repair synthesis and ligation in TC-NER.

Miscellaneous databases

PROiP35817.

Family and domain databases

Gene3Di1.20.920.10. 2 hits.
InterProiIPR001487. Bromodomain.
IPR018359. Bromodomain_CS.
IPR027353. NET_dom.
[Graphical view]
PfamiPF17035. BET. 1 hit.
PF00439. Bromodomain. 2 hits.
[Graphical view]
PRINTSiPR00503. BROMODOMAIN.
SMARTiSM00297. BROMO. 2 hits.
[Graphical view]
SUPFAMiSSF47370. SSF47370. 2 hits.
PROSITEiPS00633. BROMODOMAIN_1. 2 hits.
PS50014. BROMODOMAIN_2. 2 hits.
PS51525. NET. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiBDF1_YEAST
AccessioniPrimary (citable) accession number: P35817
Secondary accession number(s): D6VZ34, Q06048
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 1, 1994
Last sequence update: July 15, 1999
Last modified: November 30, 2016
This is version 160 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 8100 molecules/cell in log phase SD medium.1 Publication

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  3. Yeast chromosome XII
    Yeast (Saccharomyces cerevisiae) chromosome XII: entries and gene names

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.