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P35817

- BDF1_YEAST

UniProt

P35817 - BDF1_YEAST

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Protein

Bromodomain-containing factor 1

Gene

BDF1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli

Functioni

Transcription factor involved in the expression of a broad class of genes including snRNAs. Required for sporulation and DNA-damage repair. Prevents the spreading of SIR silencing at telomeres and protects histone H4, but not H3, from deacetylation.8 Publications

GO - Molecular functioni

  1. chromatin binding Source: SGD
  2. core promoter binding Source: SGD
  3. lysine-acetylated histone binding Source: SGD
  4. TFIID-class transcription factor binding Source: SGD

GO - Biological processi

  1. chromatin remodeling Source: SGD
  2. DNA repair Source: SGD
  3. negative regulation of heterochromatin assembly Source: SGD
  4. positive regulation of histone exchange Source: SGD
  5. regulation of chromatin silencing at silent mating-type cassette Source: SGD
  6. regulation of chromatin silencing at telomere Source: SGD
  7. regulation of transcription by chromatin organization Source: SGD
  8. snRNA transcription Source: SGD
  9. sporulation resulting in formation of a cellular spore Source: UniProtKB-KW
Complete GO annotation...

Keywords - Biological processi

DNA damage, DNA repair, Sporulation, Transcription, Transcription regulation

Enzyme and pathway databases

BioCyciYEAST:G3O-32463-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Bromodomain-containing factor 1
Gene namesi
Name:BDF1
Ordered Locus Names:YLR399C
ORF Names:L8084.18
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
ProteomesiUP000002311: Chromosome XII

Organism-specific databases

CYGDiYLR399c.
SGDiS000004391. BDF1.

Subcellular locationi

Nucleus 2 Publications

GO - Cellular componenti

  1. nuclear chromatin Source: SGD
  2. Swr1 complex Source: SGD
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi187 – 1871Y → F: Impairs interaction with histones H3 and H4; when associated with F-354. 1 Publication
Mutagenesisi354 – 3541Y → F: Impairs interaction with histones H3 and H4; when associated with F-187. 1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 686686Bromodomain-containing factor 1PRO_0000211176Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei270 – 2701Phosphoserine2 Publications
Modified residuei429 – 4291Phosphoserine2 Publications
Modified residuei615 – 6151Phosphoserine2 Publications
Modified residuei659 – 6591Phosphoserine1 Publication

Post-translational modificationi

Phosphorylated by the casein kinase CK2 complex.4 Publications

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiP35817.
PaxDbiP35817.
PeptideAtlasiP35817.
PRIDEiP35817.

Expressioni

Gene expression databases

GenevestigatoriP35817.

Interactioni

Subunit structurei

Interacts with the TFIID subunit TAF7 and with acetylated histones H3 and H4.4 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
BDF2Q074425EBI-3493,EBI-37620
HHT2P618302EBI-3493,EBI-8098
HTZ1Q126924EBI-3493,EBI-8080
SWR1Q054713EBI-3493,EBI-22102
TAF1P466773EBI-3493,EBI-18855
TAF12Q037612EBI-3493,EBI-35097
TAF6P530402EBI-3493,EBI-18876
TAF7Q050215EBI-3493,EBI-27490
VPS71Q034332EBI-3493,EBI-27814
YAF9P539303EBI-3493,EBI-28841

Protein-protein interaction databases

BioGridi31658. 178 interactions.
DIPiDIP-1624N.
IntActiP35817. 30 interactions.
MINTiMINT-407031.
STRINGi4932.YLR399C.

Structurei

3D structure databases

ProteinModelPortaliP35817.
SMRiP35817. Positions 161-419.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini165 – 23773Bromo 1PROSITE-ProRule annotationAdd
BLAST
Domaini332 – 40473Bromo 2PROSITE-ProRule annotationAdd
BLAST
Domaini518 – 59881NETPROSITE-ProRule annotationAdd
BLAST

Coiled coil

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Coiled coili460 – 49940Sequence AnalysisAdd
BLAST

Sequence similaritiesi

Contains 2 bromo domains.PROSITE-ProRule annotation
Contains 1 NET domain.PROSITE-ProRule annotation

Keywords - Domaini

Bromodomain, Coiled coil, Repeat

Phylogenomic databases

eggNOGiCOG5076.
GeneTreeiENSGT00760000119206.
HOGENOMiHOG000248774.
InParanoidiP35817.
KOiK11684.
OMAiPKSKDIY.
OrthoDBiEOG7Z69S1.

Family and domain databases

Gene3Di1.20.920.10. 2 hits.
InterProiIPR001487. Bromodomain.
IPR018359. Bromodomain_CS.
IPR027353. NET_dom.
[Graphical view]
PfamiPF00439. Bromodomain. 2 hits.
[Graphical view]
PRINTSiPR00503. BROMODOMAIN.
SMARTiSM00297. BROMO. 2 hits.
[Graphical view]
SUPFAMiSSF47370. SSF47370. 2 hits.
PROSITEiPS00633. BROMODOMAIN_1. 2 hits.
PS50014. BROMODOMAIN_2. 2 hits.
PS51525. NET. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P35817-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MTDITPVQND VDVNGNNVND DVSSNLKRPI DQGDPSNGLA EEENPANNQL
60 70 80 90 100
HLKKARLDGD ALTSSPAGLA ENGIEGATLA ANGENGYNAT GSGAEDEQQG
110 120 130 140 150
LKKEEGGQGT KQEDLDENSK QELPMEVPKE PAPAPPPEPD MNNLPQNPIP
160 170 180 190 200
KHQQKHALLA IKAVKRLKDA RPFLQPVDPV KLDIPFYFNY IKRPMDLSTI
210 220 230 240 250
ERKLNVGAYE VPEQITEDFN LMVNNSIKFN GPNAGISQMA RNIQASFEKH
260 270 280 290 300
MLNMPAKDAP PVIAKGRRSS AQEDAPIVIR RAQTHNGRPK RTIHPPKSKD
310 320 330 340 350
IYPYESKKPK SKRLQQAMKF CQSVLKELMA KKHASYNYPF LEPVDPVSMN
360 370 380 390 400
LPTYFDYVKE PMDLGTIAKK LNDWQYQTME DFERDVRLVF KNCYTFNPDG
410 420 430 440 450
TIVNMMGHRL EEVFNSKWAD RPNLDDYDSD EDSRTQGDYD DYESEYSESD
460 470 480 490 500
IDETIITNPA IQYLEEQLAR MKVELQQLKK QELEKIRKER RLARGSKKRG
510 520 530 540 550
KRSKGRSGSK NASSKGRRDK KNKLKTVVTY DMKRIITERI NDLPTSKLER
560 570 580 590 600
AIDIIKKSMP NISEDDEVEL DLDTLDNHTI LTLYNTFFRQ YESSSGASNG
610 620 630 640 650
LDGTSGVTRD ASSLSPTSAG SRKRRSKALS QEEQSRQIEK IKNKLAILDS
660 670 680
ASPLSQNGSP GQIQSAAHNG FSSSSDDDVS SESEEE
Length:686
Mass (Da):76,978
Last modified:July 15, 1999 - v3
Checksum:i8CCD52F41F91D0DA
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti8 – 81Q → LC in CAA79377. (PubMed:7816623)Curated
Sequence conflicti93 – 942GA → R in AAA89115. (PubMed:7791775)Curated
Sequence conflicti94 – 941A → P in CAA79377. (PubMed:7816623)Curated
Sequence conflicti282 – 2821A → P in CAA79377. (PubMed:7816623)Curated
Sequence conflicti385 – 3851D → E in CAA79377. (PubMed:7816623)Curated
Sequence conflicti493 – 4931A → R in AAA35048. (PubMed:8321235)Curated

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
Z18944 Genomic DNA. Translation: CAA79377.1.
U18116 Genomic DNA. Translation: AAA89115.1.
U19729 Genomic DNA. Translation: AAB82357.1.
L13469 mRNA. Translation: AAA35048.1.
BK006945 Genomic DNA. Translation: DAA09700.1.
PIRiS55955.
RefSeqiNP_013503.1. NM_001182287.1.

Genome annotation databases

EnsemblFungiiYLR399C; YLR399C; YLR399C.
GeneIDi851115.
KEGGisce:YLR399C.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
Z18944 Genomic DNA. Translation: CAA79377.1 .
U18116 Genomic DNA. Translation: AAA89115.1 .
U19729 Genomic DNA. Translation: AAB82357.1 .
L13469 mRNA. Translation: AAA35048.1 .
BK006945 Genomic DNA. Translation: DAA09700.1 .
PIRi S55955.
RefSeqi NP_013503.1. NM_001182287.1.

3D structure databases

ProteinModelPortali P35817.
SMRi P35817. Positions 161-419.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 31658. 178 interactions.
DIPi DIP-1624N.
IntActi P35817. 30 interactions.
MINTi MINT-407031.
STRINGi 4932.YLR399C.

Proteomic databases

MaxQBi P35817.
PaxDbi P35817.
PeptideAtlasi P35817.
PRIDEi P35817.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblFungii YLR399C ; YLR399C ; YLR399C .
GeneIDi 851115.
KEGGi sce:YLR399C.

Organism-specific databases

CYGDi YLR399c.
SGDi S000004391. BDF1.

Phylogenomic databases

eggNOGi COG5076.
GeneTreei ENSGT00760000119206.
HOGENOMi HOG000248774.
InParanoidi P35817.
KOi K11684.
OMAi PKSKDIY.
OrthoDBi EOG7Z69S1.

Enzyme and pathway databases

BioCyci YEAST:G3O-32463-MONOMER.

Miscellaneous databases

NextBioi 967833.
PROi P35817.

Gene expression databases

Genevestigatori P35817.

Family and domain databases

Gene3Di 1.20.920.10. 2 hits.
InterProi IPR001487. Bromodomain.
IPR018359. Bromodomain_CS.
IPR027353. NET_dom.
[Graphical view ]
Pfami PF00439. Bromodomain. 2 hits.
[Graphical view ]
PRINTSi PR00503. BROMODOMAIN.
SMARTi SM00297. BROMO. 2 hits.
[Graphical view ]
SUPFAMi SSF47370. SSF47370. 2 hits.
PROSITEi PS00633. BROMODOMAIN_1. 2 hits.
PS50014. BROMODOMAIN_2. 2 hits.
PS51525. NET. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "The yeast BDF1 gene encodes a transcription factor involved in the expression of a broad class of genes including snRNAs."
    Lygerou Z., Conesa C., Lesage P., Swanson R.N., Ruet A., Carlson M., Sentenac A., Seraphin B.
    Nucleic Acids Res. 22:5332-5340(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION.
    Strain: ATCC 204508 / S288c.
  2. "Bdf1, a yeast chromosomal protein required for sporulation."
    Chua P., Roeder G.S.
    Mol. Cell. Biol. 15:3685-3696(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION, SUBCELLULAR LOCATION.
  3. "The nucleotide sequence of Saccharomyces cerevisiae chromosome XII."
    Johnston M., Hillier L.W., Riles L., Albermann K., Andre B., Ansorge W., Benes V., Brueckner M., Delius H., Dubois E., Duesterhoeft A., Entian K.-D., Floeth M., Goffeau A., Hebling U., Heumann K., Heuss-Neitzel D., Hilbert H.
    , Hilger F., Kleine K., Koetter P., Louis E.J., Messenguy F., Mewes H.-W., Miosga T., Moestl D., Mueller-Auer S., Nentwich U., Obermaier B., Piravandi E., Pohl T.M., Portetelle D., Purnelle B., Rechmann S., Rieger M., Rinke M., Rose M., Scharfe M., Scherens B., Scholler P., Schwager C., Schwarz S., Underwood A.P., Urrestarazu L.A., Vandenbol M., Verhasselt P., Vierendeels F., Voet M., Volckaert G., Voss H., Wambutt R., Wedler E., Wedler H., Zimmermann F.K., Zollner A., Hani J., Hoheisel J.D.
    Nature 387:87-90(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  4. Cited for: GENOME REANNOTATION.
    Strain: ATCC 204508 / S288c.
  5. "Evidence that the SKI antiviral system of Saccharomyces cerevisiae acts by blocking expression of viral mRNA."
    Widner W.R., Wickner R.B.
    Mol. Cell. Biol. 13:4331-4341(1993) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 471-686.
  6. Cited for: DOMAIN BROMODOMAIN.
  7. "Bromodomain factor 1 corresponds to a missing piece of yeast TFIID."
    Matangkasombut O., Buratowski R.M., Swilling N.W., Buratowski S.
    Genes Dev. 14:951-962(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, INTERACTION WITH TAF7.
  8. Cited for: INTERACTION WITH HISTONES H3 AND H4.
  9. "A genome-wide screen for methyl methanesulfonate-sensitive mutants reveals genes required for S phase progression in the presence of DNA damage."
    Chang M., Bellaoui M., Boone C., Brown G.W.
    Proc. Natl. Acad. Sci. U.S.A. 99:16934-16939(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  10. "Different sensitivities of bromodomain factors 1 and 2 to histone H4 acetylation."
    Matangkasombut O., Buratowski S.
    Mol. Cell 11:353-363(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, INTERACTION WITH HISTONES H3 AND H4.
  11. "Bromodomains mediate an acetyl-histone encoded antisilencing function at heterochromatin boundaries."
    Ladurner A.G., Inouye C., Jain R., Tjian R.
    Mol. Cell 11:365-376(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, INTERACTION WITH ACETYLATED HISTONES H3 AND H4, MUTAGENESIS OF TYR-187 AND TYR-354.
  12. Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
  13. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
  14. "Precise nucleosome positioning and the TATA box dictate requirements for the histone H4 tail and the bromodomain factor Bdf1."
    Martinez-Campa C., Politis P., Moreau J.-L., Kent N., Goodall J., Mellor J., Goding C.R.
    Mol. Cell 15:69-81(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  15. "Bromodomain factor 1 (Bdf1) is phosphorylated by protein kinase CK2."
    Sawa C., Nedea E., Krogan N., Wada T., Handa H., Greenblatt J., Buratowski S.
    Mol. Cell. Biol. 24:4734-4742(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION BY THE CK2 PROTEIN KINASE COMPLEX.
  16. "The bromodomain-containing protein Bdf1p acts as a phenotypic and transcriptional multicopy suppressor of YAF9 deletion in yeast."
    Bianchi M.M., Costanzo G., Chelstowska A., Grabowska D., Mazzoni C., Piccinni E., Cavalli A., Ciceroni F., Rytka J., Slonimski P.P., Frontali L., Negri R.
    Mol. Microbiol. 53:953-968(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  17. "Quantitative phosphoproteomics applied to the yeast pheromone signaling pathway."
    Gruhler A., Olsen J.V., Mohammed S., Mortensen P., Faergeman N.J., Mann M., Jensen O.N.
    Mol. Cell. Proteomics 4:310-327(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Strain: YAL6B.
  18. "Large-scale phosphorylation analysis of alpha-factor-arrested Saccharomyces cerevisiae."
    Li X., Gerber S.A., Rudner A.D., Beausoleil S.A., Haas W., Villen J., Elias J.E., Gygi S.P.
    J. Proteome Res. 6:1190-1197(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-270 AND SER-429, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Strain: ADR376.
  19. "Analysis of phosphorylation sites on proteins from Saccharomyces cerevisiae by electron transfer dissociation (ETD) mass spectrometry."
    Chi A., Huttenhower C., Geer L.Y., Coon J.J., Syka J.E.P., Bai D.L., Shabanowitz J., Burke D.J., Troyanskaya O.G., Hunt D.F.
    Proc. Natl. Acad. Sci. U.S.A. 104:2193-2198(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  20. "A multidimensional chromatography technology for in-depth phosphoproteome analysis."
    Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.
    Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-615 AND SER-659, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  21. "Global analysis of Cdk1 substrate phosphorylation sites provides insights into evolution."
    Holt L.J., Tuch B.B., Villen J., Johnson A.D., Gygi S.P., Morgan D.O.
    Science 325:1682-1686(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-270; SER-429 AND SER-615, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

Entry informationi

Entry nameiBDF1_YEAST
AccessioniPrimary (citable) accession number: P35817
Secondary accession number(s): D6VZ34, Q06048
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 1, 1994
Last sequence update: July 15, 1999
Last modified: October 29, 2014
This is version 139 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 8100 molecules/cell in log phase SD medium.1 Publication

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  3. Yeast chromosome XII
    Yeast (Saccharomyces cerevisiae) chromosome XII: entries and gene names

External Data

Dasty 3