Reviewed, UniProtKB/Swiss-Prot P35816 (PDP1_BOVIN)

Names and origin

Protein names

Recommended name:
    [Pyruvate dehydrogenase [acetyl-transferring]]-phosphatase 1, mitochondrial
      Short name=PDP 1
    EC=3.1.3.43
Alternative name(s):
    Pyruvate dehydrogenase phosphatase, catalytic subunit 1
      Short name=PDPC 1
    Protein phosphatase 2C

Gene names

Name:	PDP1
Synonyms:	PDP, PPM2C

Organism

Bos taurus (Bovine)

Taxonomic identifier

9913 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Laurasiatheria › Cetartiodactyla › Ruminantia › Pecora › Bovidae › Bovinae › Bos

Protein attributes

Sequence length	538 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Catalyzes the dephosphorylation and concomitant reactivation of the alpha subunit of the E1 component of the pyruvate dehydrogenase complex.
Catalytic activity	[Pyruvate dehydrogenase (acetyl-transferring)] phosphate + H2O = [pyruvate dehydrogenase (acetyl-transferring)] + phosphate.
Cofactor	Binds 2 magnesium ions per subunit By similarity.
Subunit structure	Heterodimer of a catalytic (PDP1) and a regulatory (PDPR) subunit. Ref.2
Subcellular location	Mitochondrion matrix.
Sequence similarities	Belongs to the PP2C family.

Ontologies

Keywords
Cellular component	Mitochondrion
Domain	Transit peptide
Ligand	Calcium Magnesium Metal-binding
Molecular function	Hydrolase Protein phosphatase
Technical term	Direct protein sequencing
Gene Ontology (GO)
Biological process	protein amino acid dephosphorylation Inferred from electronic annotation. Source: InterPro
Cellular component	mitochondrial matrix Inferred from electronic annotation. Source: UniProtKB-SubCell protein serine/threonine phosphatase complex Inferred from electronic annotation. Source: InterPro
Molecular function	[pyruvate dehydrogenase (lipoamide)] phosphatase activity Inferred from electronic annotation. Source: EC calcium ion binding Inferred from direct assay. Source: UniProtKB magnesium ion binding Non-traceable author statement. Source: UniProtKB protein serine/threonine phosphatase activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Transit peptide	1 – 71	71	Mitochondrion
	Chain	72 – 538	467	[Pyruvate dehydrogenase [acetyl-transferring]]-phosphatase 1, mitochondrial		PRO_0000025418
Sites
	Metal binding	144	1	Magnesium 1 By similarity
	Metal binding	144	1	Magnesium 2 By similarity
	Metal binding	145	1	Magnesium 1; via carbonyl oxygen By similarity
	Metal binding	418	1	Magnesium 2 By similarity

Sequences

	Sequence		Length	Mass (Da)
	P35816-1 [UniParc]. Last modified June 1, 1994. Version 1. Checksum: 1A1C219AD8C3DAE3	FASTA	538	61,184
	10 20 30 40 50 60 MPAPTQLFFP LIRNCELSRI YGTACYCHHK HLCCSPPYIP QSRPRYTPHP AYATFYRPKE 70 80 90 100 110 120 SWWQYTQGRR YASTPQKFYL TPPQVNSILK ANEYSFKVPE FDGKNVSSVL GFDSNQLPAN 130 140 150 160 170 180 APIEDRRSAA TCLQTRGMLL GVFDGHAGCA CSQAVSERLF YYIAVSLLPH ETLLEIENAV 190 200 210 220 230 240 ESGRALLPIL QWHKHPNDYF SKEASKLYFN SLRTYWQELI DLNTGESTDI DVKEALINAF 250 260 270 280 290 300 KRLDNDISLE AQVGDPNSFL NYLVLRVAFS GATACVAHVD GVDLHVANTG DSRAMLGVQE 310 320 330 340 350 360 EDGSWSAVTL SNDHNAQNER EVERLKLEHP KNEAKSVVKQ DRLLGLLMPF RAFGDVKFKW 370 380 390 400 410 420 SIDLQKRVIE SGPDQLNDNE YTKFIPPNYY TPPYLTAEPE VTYHRLRPQD KFLVLATDGL 430 440 450 460 470 480 WETMHRQDVV RIVGEYLTGM HHQQPIAVGG YKVTLGQMHG LLTERRAKMS SVFEDQNAAT 490 500 510 520 530 HLIRHAVGNN EFGAVDHERL SKMLSLPEEL ARMYRDDITI IVVQFNSHVV GAYQNQEQ

References

[1]	"Molecular cloning and expression of the catalytic subunit of bovine pyruvate dehydrogenase phosphatase and sequence similarity with protein phosphatase 2C." Lawson J.E., Niu X.-D., Browning K.S., Trong H.L., Yan J., Reed L.J. Biochemistry 32:8987-8993(1993) [PubMed: 8396421] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], PARTIAL PROTEIN SEQUENCE.
[2]	"Cloning, expression, and properties of the regulatory subunit of bovine pyruvate dehydrogenase phosphatase." Lawson J.E., Park S.H., Mattison A.R., Yan J., Reed L.J. J. Biol. Chem. 272:31625-31629(1997) [PubMed: 9395502] [Abstract] Cited for: SUBUNIT.

Cross-references

Sequence databases
EMBLGenBankDDBJ	L18966 mRNA. Translation: AAA30697.1. Different initiation.
IPI	IPI00837836.
PIR	A48692.
RefSeq	NP_776374.1.
UniGene	Bt.3889
3D structure databases
ModBase	Search...
Genome annotation databases
Ensembl	ENSBTAG00000000199. Bos taurus. [Contig view]
GeneID	280891.
KEGG	bta:280891.
Phylogenomic databases
HOVERGEN	P35816.
Enzyme and pathway databases
BRENDA	3.1.3.43. 251.
Family and domain databases
InterPro	IPR015655. PP2C. IPR001932. PP2C-related. IPR000222. PP2C_Mn2_Asp60_BS. IPR014045. PP2C_N. [Graphical view]
Gene3D	G3DSA:3.60.40.10. PP2C-related. 1 hit.
PANTHER	PTHR13832. PP2C. 1 hit.
Pfam	PF00481. PP2C. 1 hit. [Graphical view]
SMART	SM00331. PP2C_SIG. 1 hit. SM00332. PP2Cc. 1 hit. [Graphical view]
PROSITE	PS01032. PP2C. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

PDP1_BOVIN

Accession

Primary (citable) accession number: P35816

Entry history

Integrated into UniProtKB/Swiss-Prot:	June 1, 1994
Last sequence update:	June 1, 1994
Last modified:	February 10, 2009
This is version 69 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)