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Protein

[Pyruvate dehydrogenase [acetyl-transferring]]-phosphatase 1, mitochondrial

Gene

PDP1

Organism
Bos taurus (Bovine)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Catalyzes the dephosphorylation and concomitant reactivation of the alpha subunit of the E1 component of the pyruvate dehydrogenase complex.

Catalytic activityi

[Pyruvate dehydrogenase (acetyl-transferring)] phosphate + H2O = [pyruvate dehydrogenase (acetyl-transferring)] + phosphate.

Cofactori

Mg2+By similarityNote: Binds 2 magnesium ions per subunit.By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi144Magnesium 1By similarity1
Metal bindingi144Magnesium 2By similarity1
Metal bindingi145Magnesium 1; via carbonyl oxygenBy similarity1
Metal bindingi418Magnesium 2By similarity1
Metal bindingi516Magnesium 1By similarity1

GO - Molecular functioni

  • [pyruvate dehydrogenase (lipoamide)] phosphatase activity Source: UniProtKB-EC
  • calcium ion binding Source: UniProtKB
  • magnesium ion binding Source: UniProtKB
  • protein serine/threonine phosphatase activity Source: InterPro

GO - Biological processi

  • dephosphorylation Source: UniProtKB
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Protein phosphatase

Keywords - Ligandi

Calcium, Magnesium, Metal-binding

Enzyme and pathway databases

BRENDAi3.1.3.43. 908.
ReactomeiR-BTA-204174. Regulation of pyruvate dehydrogenase (PDH) complex.

Names & Taxonomyi

Protein namesi
Recommended name:
[Pyruvate dehydrogenase [acetyl-transferring]]-phosphatase 1, mitochondrial (EC:3.1.3.43)
Short name:
PDP 1
Alternative name(s):
Protein phosphatase 2C
Pyruvate dehydrogenase phosphatase catalytic subunit 1
Short name:
PDPC 1
Gene namesi
Name:PDP1
Synonyms:PDP, PPM2C
OrganismiBos taurus (Bovine)
Taxonomic identifieri9913 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCetartiodactylaRuminantiaPecoraBovidaeBovinaeBos
Proteomesi
  • UP000009136 Componenti: Chromosome 14

Subcellular locationi

GO - Cellular componenti

  • mitochondrial matrix Source: UniProtKB-SubCell
  • mitochondrion Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Mitochondrion

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transit peptidei1 – 71MitochondrionAdd BLAST71
ChainiPRO_000002541872 – 538[Pyruvate dehydrogenase [acetyl-transferring]]-phosphatase 1, mitochondrialAdd BLAST467

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei202N6-acetyllysineBy similarity1

Keywords - PTMi

Acetylation

Proteomic databases

PaxDbiP35816.
PeptideAtlasiP35816.
PRIDEiP35816.

Expressioni

Gene expression databases

BgeeiENSBTAG00000000199.
ExpressionAtlasiP35816. differential.

Interactioni

Subunit structurei

Heterodimer of a catalytic (PDP1) and a regulatory (PDPR) subunit.1 Publication

Protein-protein interaction databases

STRINGi9913.ENSBTAP00000043214.

Structurei

Secondary structure

1538
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi85 – 89Combined sources5
Turni90 – 92Combined sources3
Beta strandi94 – 97Combined sources4
Beta strandi107 – 117Combined sources11
Beta strandi120 – 122Combined sources3
Beta strandi125 – 136Combined sources12
Beta strandi138 – 149Combined sources12
Helixi150 – 166Combined sources17
Helixi170 – 178Combined sources9
Beta strandi190 – 192Combined sources3
Helixi205 – 220Combined sources16
Helixi232 – 253Combined sources22
Helixi258 – 269Combined sources12
Beta strandi270 – 272Combined sources3
Beta strandi274 – 280Combined sources7
Beta strandi283 – 291Combined sources9
Beta strandi293 – 299Combined sources7
Beta strandi305 – 310Combined sources6
Helixi319 – 327Combined sources9
Helixi331 – 333Combined sources3
Turni334 – 336Combined sources3
Beta strandi337 – 339Combined sources3
Turni344 – 346Combined sources3
Beta strandi347 – 351Combined sources5
Helixi356 – 358Combined sources3
Helixi362 – 368Combined sources7
Beta strandi390 – 392Combined sources3
Beta strandi400 – 405Combined sources6
Beta strandi410 – 416Combined sources7
Helixi418 – 421Combined sources4
Helixi426 – 437Combined sources12
Helixi478 – 486Combined sources9
Helixi511 – 514Combined sources4
Beta strandi518 – 525Combined sources8
Helixi527 – 533Combined sources7

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
3MQ3X-ray2.80A72-538[»]
3N3CX-ray2.10A72-538[»]
ProteinModelPortaliP35816.
SMRiP35816.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP35816.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini109 – 525PPM-type phosphatasePROSITE-ProRule annotationAdd BLAST417

Sequence similaritiesi

Belongs to the PP2C family.Curated
Contains 1 PPM-type phosphatase domain.PROSITE-ProRule annotation

Keywords - Domaini

Transit peptide

Phylogenomic databases

eggNOGiKOG0700. Eukaryota.
COG0631. LUCA.
GeneTreeiENSGT00390000006874.
HOGENOMiHOG000220821.
HOVERGENiHBG008162.
InParanoidiP35816.
KOiK01102.

Family and domain databases

Gene3Di3.60.40.10. 2 hits.
InterProiIPR015655. PP2C.
IPR000222. PP2C_BS.
IPR001932. PPM-type_phosphatase_dom.
[Graphical view]
PANTHERiPTHR13832. PTHR13832. 5 hits.
PfamiPF00481. PP2C. 1 hit.
[Graphical view]
SMARTiSM00332. PP2Cc. 1 hit.
[Graphical view]
SUPFAMiSSF81606. SSF81606. 3 hits.
PROSITEiPS01032. PPM_1. 1 hit.
PS51746. PPM_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P35816-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MPAPTQLFFP LIRNCELSRI YGTACYCHHK HLCCSPPYIP QSRPRYTPHP
60 70 80 90 100
AYATFYRPKE SWWQYTQGRR YASTPQKFYL TPPQVNSILK ANEYSFKVPE
110 120 130 140 150
FDGKNVSSVL GFDSNQLPAN APIEDRRSAA TCLQTRGMLL GVFDGHAGCA
160 170 180 190 200
CSQAVSERLF YYIAVSLLPH ETLLEIENAV ESGRALLPIL QWHKHPNDYF
210 220 230 240 250
SKEASKLYFN SLRTYWQELI DLNTGESTDI DVKEALINAF KRLDNDISLE
260 270 280 290 300
AQVGDPNSFL NYLVLRVAFS GATACVAHVD GVDLHVANTG DSRAMLGVQE
310 320 330 340 350
EDGSWSAVTL SNDHNAQNER EVERLKLEHP KNEAKSVVKQ DRLLGLLMPF
360 370 380 390 400
RAFGDVKFKW SIDLQKRVIE SGPDQLNDNE YTKFIPPNYY TPPYLTAEPE
410 420 430 440 450
VTYHRLRPQD KFLVLATDGL WETMHRQDVV RIVGEYLTGM HHQQPIAVGG
460 470 480 490 500
YKVTLGQMHG LLTERRAKMS SVFEDQNAAT HLIRHAVGNN EFGAVDHERL
510 520 530
SKMLSLPEEL ARMYRDDITI IVVQFNSHVV GAYQNQEQ
Length:538
Mass (Da):61,184
Last modified:June 1, 1994 - v1
Checksum:i1A1C219AD8C3DAE3
GO

Sequence cautioni

The sequence AAA30697 differs from that shown. Reason: Erroneous initiation.Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L18966 mRNA. Translation: AAA30697.1. Different initiation.
PIRiA48692.
RefSeqiNP_001193282.1. NM_001206353.1.
XP_005215704.1. XM_005215647.3.
XP_005215707.1. XM_005215650.3.
XP_010810497.1. XM_010812195.2.
UniGeneiBt.3889.

Genome annotation databases

EnsembliENSBTAT00000000233; ENSBTAP00000000233; ENSBTAG00000000199.
GeneIDi280891.
KEGGibta:280891.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L18966 mRNA. Translation: AAA30697.1. Different initiation.
PIRiA48692.
RefSeqiNP_001193282.1. NM_001206353.1.
XP_005215704.1. XM_005215647.3.
XP_005215707.1. XM_005215650.3.
XP_010810497.1. XM_010812195.2.
UniGeneiBt.3889.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
3MQ3X-ray2.80A72-538[»]
3N3CX-ray2.10A72-538[»]
ProteinModelPortaliP35816.
SMRiP35816.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi9913.ENSBTAP00000043214.

Proteomic databases

PaxDbiP35816.
PeptideAtlasiP35816.
PRIDEiP35816.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSBTAT00000000233; ENSBTAP00000000233; ENSBTAG00000000199.
GeneIDi280891.
KEGGibta:280891.

Organism-specific databases

CTDi54704.

Phylogenomic databases

eggNOGiKOG0700. Eukaryota.
COG0631. LUCA.
GeneTreeiENSGT00390000006874.
HOGENOMiHOG000220821.
HOVERGENiHBG008162.
InParanoidiP35816.
KOiK01102.

Enzyme and pathway databases

BRENDAi3.1.3.43. 908.
ReactomeiR-BTA-204174. Regulation of pyruvate dehydrogenase (PDH) complex.

Miscellaneous databases

EvolutionaryTraceiP35816.

Gene expression databases

BgeeiENSBTAG00000000199.
ExpressionAtlasiP35816. differential.

Family and domain databases

Gene3Di3.60.40.10. 2 hits.
InterProiIPR015655. PP2C.
IPR000222. PP2C_BS.
IPR001932. PPM-type_phosphatase_dom.
[Graphical view]
PANTHERiPTHR13832. PTHR13832. 5 hits.
PfamiPF00481. PP2C. 1 hit.
[Graphical view]
SMARTiSM00332. PP2Cc. 1 hit.
[Graphical view]
SUPFAMiSSF81606. SSF81606. 3 hits.
PROSITEiPS01032. PPM_1. 1 hit.
PS51746. PPM_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiPDP1_BOVIN
AccessioniPrimary (citable) accession number: P35816
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 1, 1994
Last sequence update: June 1, 1994
Last modified: November 30, 2016
This is version 128 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.