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P35815

- PPM1B_RAT

UniProt

P35815 - PPM1B_RAT

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Protein

Protein phosphatase 1B

Gene
Ppm1b, Pp2c2, Pppm1b
Organism
Rattus norvegicus (Rat)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at transcript leveli

Functioni

Enzyme with a broad specificity. Dephosphorylates PRKAA1 and PRKAA2. Inhibits TBK1-mediated antiviral signaling by dephosphorylating it at 'Ser-172'. Plays an important role in the termination of TNF-alpha-mediated NF-kappa-B activation through dephosphorylating and inactivating IKBKB/IKKB By similarity.

Catalytic activityi

[a protein]-serine/threonine phosphate + H2O = [a protein]-serine/threonine + phosphate.

Cofactori

Binds 2 magnesium or manganese ions per subunit By similarity.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi60 – 601Manganese 1 By similarity
Metal bindingi60 – 601Manganese 2 By similarity
Metal bindingi61 – 611Manganese 1; via carbonyl oxygen By similarity
Metal bindingi243 – 2431Manganese 2 By similarity
Metal bindingi286 – 2861Manganese 2 By similarity

GO - Molecular functioni

  1. magnesium ion binding Source: InterPro
  2. manganese ion binding Source: InterPro
  3. protein serine/threonine phosphatase activity Source: InterPro

GO - Biological processi

  1. negative regulation of defense response to virus Source: UniProtKB
  2. negative regulation of I-kappaB kinase/NF-kappaB signaling Source: UniProtKB
  3. negative regulation of interferon-beta production Source: UniProtKB
  4. negative regulation of NF-kappaB import into nucleus Source: UniProtKB
  5. N-terminal protein myristoylation Source: UniProtKB
  6. protein dephosphorylation Source: UniProtKB
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Protein phosphatase

Keywords - Ligandi

Magnesium, Manganese, Metal-binding

Enzyme and pathway databases

ReactomeiREACT_198999. ISG15 antiviral mechanism.

Names & Taxonomyi

Protein namesi
Recommended name:
Protein phosphatase 1B (EC:3.1.3.16)
Alternative name(s):
Protein phosphatase 2C isoform beta
Short name:
PP2C-beta
Gene namesi
Name:Ppm1b
Synonyms:Pp2c2, Pppm1b
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
ProteomesiUP000002494: Chromosome 6

Organism-specific databases

RGDi3374. Ppm1b.

Subcellular locationi

Cytoplasmcytosol By similarity. Membrane By similarity
Note: Weakly associates at the membrane and N-myristoylation mediates the membrane localization By similarity.

GO - Cellular componenti

  1. cytosol Source: UniProtKB
  2. membrane Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methioninei1 – 11Removed By similarity
Chaini2 – 390389Protein phosphatase 1BPRO_0000057748Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Lipidationi2 – 21N-myristoyl glycine By similarity
Cross-linki12 – 12Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ISG15) By similarity

Post-translational modificationi

Isgylation negatively regulates its activity By similarity.
N-myristoylation is essential for the recognition of its substrates for dephosphorylation By similarity.

Keywords - PTMi

Isopeptide bond, Lipoprotein, Myristate, Ubl conjugation

Proteomic databases

PaxDbiP35815.
PRIDEiP35815.

Expressioni

Gene expression databases

GenevestigatoriP35815.

Interactioni

Subunit structurei

Monomer By similarity. Interacts with PAK6 By similarity. Interacts with the phosphorylated form of IKBKB/IKKB By similarity.

Structurei

3D structure databases

ProteinModelPortaliP35815.

Family & Domainsi

Sequence similaritiesi

Belongs to the PP2C family.

Phylogenomic databases

eggNOGiCOG0631.
GeneTreeiENSGT00740000115384.
HOGENOMiHOG000233895.
HOVERGENiHBG053647.
KOiK04461.

Family and domain databases

Gene3Di1.10.10.430. 1 hit.
3.60.40.10. 1 hit.
InterProiIPR001932. PP2C-like_dom.
IPR012911. PP2C_C.
IPR000222. PP2C_Mn2_Asp60_BS.
IPR015655. Protein_Pase_2C.
[Graphical view]
PANTHERiPTHR13832. PTHR13832. 1 hit.
PfamiPF00481. PP2C. 1 hit.
PF07830. PP2C_C. 1 hit.
[Graphical view]
SMARTiSM00331. PP2C_SIG. 1 hit.
SM00332. PP2Cc. 1 hit.
[Graphical view]
SUPFAMiSSF81601. SSF81601. 1 hit.
SSF81606. SSF81606. 1 hit.
PROSITEiPS01032. PP2C. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 2 isoformsi produced by alternative splicing. Align

Note: Additional isoforms seem to exist. Isoforms appear to differ in their C-terminus.

Isoform 1 (identifier: P35815-1) [UniParc]FASTAAdd to Basket

Also known as: Beta-1

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

MGAFLDKPKT EKHNAHGAGN GLRYGLSSMQ GWRVEMEDAH TAVVGIPHGL    50
EDWSFFAVYD GHAGSRVANY CSTHLLEHIT TNEDFRAADK SGFALEPSVE 100
NVKTGIRTGF LKIDEYMRNF SDLRNGMDRS GSTAVGVMIS PTHIYFINCG 150
DSRAVLCRNG QVCFSTQDHK PCNPMEKERI QNAGGSVMIQ RVNGSLAVSR 200
ALGDYDYKCV DGKGPTEQLV SPEPEVYEIL RAEEDEFVVL ACDGIWDVMS 250
NEELCEFVNS RLEVSDDLEN VCNWVVDTCL HKGSRDNMSI VLVCFANAPK 300
VSDEAVKRDL ELDKHLESRV EEIMQKSGEE GMPDLAHVMR ILSAENIPNL 350
PPGGGLAGKR NVIEAVYSRL NPNKDNDGGA GDLEDSLVAL 390
Length:390
Mass (Da):42,889
Last modified:June 1, 1994 - v1
Checksum:iD147615BC2FA140B
GO
Isoform 2 (identifier: P35815-2) [UniParc]FASTAAdd to Basket

Also known as: Beta-MPP

The sequence of this isoform differs from the canonical sequence as follows:
     379-390: GAGDLEDSLVAL → FYQPSTPYSDNVSYYEWQT

Show »
Length:397
Mass (Da):44,105
Checksum:iE4A7846C3FE787F6
GO

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei379 – 39012GAGDL…SLVAL → FYQPSTPYSDNVSYYEWQT in isoform 2. VSP_005093Add
BLAST

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
S90449 mRNA. Translation: AAB21898.1.
S74572 mRNA. Translation: AAB33430.1.
AJ271837 mRNA. Translation: CAC28067.1.
PIRiJC2524.
S20392.
RefSeqiNP_149087.1. NM_033096.2. [P35815-1]
UniGeneiRn.4143.

Genome annotation databases

EnsembliENSRNOT00000048656; ENSRNOP00000048342; ENSRNOG00000030667. [P35815-1]
GeneIDi24667.
KEGGirno:24667.
UCSCiRGD:3374. rat. [P35815-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
S90449 mRNA. Translation: AAB21898.1 .
S74572 mRNA. Translation: AAB33430.1 .
AJ271837 mRNA. Translation: CAC28067.1 .
PIRi JC2524.
S20392.
RefSeqi NP_149087.1. NM_033096.2. [P35815-1 ]
UniGenei Rn.4143.

3D structure databases

ProteinModelPortali P35815.
ModBasei Search...
MobiDBi Search...

Proteomic databases

PaxDbi P35815.
PRIDEi P35815.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENSRNOT00000048656 ; ENSRNOP00000048342 ; ENSRNOG00000030667 . [P35815-1 ]
GeneIDi 24667.
KEGGi rno:24667.
UCSCi RGD:3374. rat. [P35815-1 ]

Organism-specific databases

CTDi 5495.
RGDi 3374. Ppm1b.

Phylogenomic databases

eggNOGi COG0631.
GeneTreei ENSGT00740000115384.
HOGENOMi HOG000233895.
HOVERGENi HBG053647.
KOi K04461.

Enzyme and pathway databases

Reactomei REACT_198999. ISG15 antiviral mechanism.

Miscellaneous databases

NextBioi 604030.
PROi P35815.

Gene expression databases

Genevestigatori P35815.

Family and domain databases

Gene3Di 1.10.10.430. 1 hit.
3.60.40.10. 1 hit.
InterProi IPR001932. PP2C-like_dom.
IPR012911. PP2C_C.
IPR000222. PP2C_Mn2_Asp60_BS.
IPR015655. Protein_Pase_2C.
[Graphical view ]
PANTHERi PTHR13832. PTHR13832. 1 hit.
Pfami PF00481. PP2C. 1 hit.
PF07830. PP2C_C. 1 hit.
[Graphical view ]
SMARTi SM00331. PP2C_SIG. 1 hit.
SM00332. PP2Cc. 1 hit.
[Graphical view ]
SUPFAMi SSF81601. SSF81601. 1 hit.
SSF81606. SSF81606. 1 hit.
PROSITEi PS01032. PP2C. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Molecular cloning and primary structure of a protein phosphatase 2C isoform."
    Wenk J., Trompeter H.-I., Pettrich K.-G., Cohen P.T.W., Campbell D.G., Mieskes G.
    FEBS Lett. 297:135-138(1992) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
    Tissue: Liver.
  2. "Monoclonal anti-FLAG antibodies react with a new isoform of rat Mg2+ dependent protein phosphatase beta."
    Schafer K., Braun T.
    Biochem. Biophys. Res. Commun. 207:708-714(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 2).
    Tissue: Brain.
  3. "Protein phosphatase 1B. Cloning and characterization of two major transcripts generated by alternative use of 3' exons."
    Seroussi E., Shani N., Hayut A., Faier S., Ben-Meir D., Divinski I., Smorodinsky N.I., Lavi S.
    Submitted (FEB-2000) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).

Entry informationi

Entry nameiPPM1B_RAT
AccessioniPrimary (citable) accession number: P35815
Secondary accession number(s): Q546R0, Q64046
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 1, 1994
Last sequence update: June 1, 1994
Last modified: September 3, 2014
This is version 112 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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