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P35815 (PPM1B_RAT) Reviewed, UniProtKB/Swiss-Prot

Last modified March 19, 2014. Version 110. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Protein phosphatase 1B

EC=3.1.3.16
Alternative name(s):
Protein phosphatase 2C isoform beta
Short name=PP2C-beta
Gene names
Name:Ppm1b
Synonyms:Pp2c2, Pppm1b
OrganismRattus norvegicus (Rat) [Reference proteome]
Taxonomic identifier10116 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus

Protein attributes

Sequence length390 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at transcript level

General annotation (Comments)

Function

Enzyme with a broad specificity. Dephosphorylates PRKAA1 and PRKAA2. Inhibits TBK1-mediated antiviral signaling by dephosphorylating it at 'Ser-172'. Plays an important role in the termination of TNF-alpha-mediated NF-kappa-B activation through dephosphorylating and inactivating IKBKB/IKKB By similarity.

Catalytic activity

[a protein]-serine/threonine phosphate + H2O = [a protein]-serine/threonine + phosphate.

Cofactor

Binds 2 magnesium or manganese ions per subunit By similarity.

Subunit structure

Monomer By similarity. Interacts with PAK6 By similarity. Interacts with the phosphorylated form of IKBKB/IKKB By similarity.

Subcellular location

Cytoplasmcytosol By similarity. Membrane By similarity. Note: Weakly associates at the membrane and N-myristoylation mediates the membrane localization By similarity.

Post-translational modification

Isgylation negatively regulates its activity By similarity.

N-myristoylation is essential for the recognition of its substrates for dephosphorylation By similarity.

Sequence similarities

Belongs to the PP2C family.

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]

Note: Additional isoforms seem to exist. Isoforms appear to differ in their C-terminus.
Isoform 1 (identifier: P35815-1)

Also known as: Beta-1;

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Isoform 2 (identifier: P35815-2)

Also known as: Beta-MPP;

The sequence of this isoform differs from the canonical sequence as follows:
     379-390: GAGDLEDSLVAL → FYQPSTPYSDNVSYYEWQT

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Initiator methionine11Removed By similarity
Chain2 – 390389Protein phosphatase 1B
PRO_0000057748

Sites

Metal binding601Manganese 1 By similarity
Metal binding601Manganese 2 By similarity
Metal binding611Manganese 1; via carbonyl oxygen By similarity
Metal binding2431Manganese 2 By similarity
Metal binding2861Manganese 2 By similarity

Amino acid modifications

Lipidation21N-myristoyl glycine By similarity
Cross-link12Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ISG15) By similarity

Natural variations

Alternative sequence379 – 39012GAGDL…SLVAL → FYQPSTPYSDNVSYYEWQT in isoform 2.
VSP_005093

Sequences

Sequence LengthMass (Da)Tools
Isoform 1 (Beta-1) [UniParc].

Last modified June 1, 1994. Version 1.
Checksum: D147615BC2FA140B

FASTA39042,889
        10         20         30         40         50         60 
MGAFLDKPKT EKHNAHGAGN GLRYGLSSMQ GWRVEMEDAH TAVVGIPHGL EDWSFFAVYD 

        70         80         90        100        110        120 
GHAGSRVANY CSTHLLEHIT TNEDFRAADK SGFALEPSVE NVKTGIRTGF LKIDEYMRNF 

       130        140        150        160        170        180 
SDLRNGMDRS GSTAVGVMIS PTHIYFINCG DSRAVLCRNG QVCFSTQDHK PCNPMEKERI 

       190        200        210        220        230        240 
QNAGGSVMIQ RVNGSLAVSR ALGDYDYKCV DGKGPTEQLV SPEPEVYEIL RAEEDEFVVL 

       250        260        270        280        290        300 
ACDGIWDVMS NEELCEFVNS RLEVSDDLEN VCNWVVDTCL HKGSRDNMSI VLVCFANAPK 

       310        320        330        340        350        360 
VSDEAVKRDL ELDKHLESRV EEIMQKSGEE GMPDLAHVMR ILSAENIPNL PPGGGLAGKR 

       370        380        390 
NVIEAVYSRL NPNKDNDGGA GDLEDSLVAL 

« Hide

Isoform 2 (Beta-MPP) [UniParc].

Checksum: E4A7846C3FE787F6
Show »

FASTA39744,105

References

[1]"Molecular cloning and primary structure of a protein phosphatase 2C isoform."
Wenk J., Trompeter H.-I., Pettrich K.-G., Cohen P.T.W., Campbell D.G., Mieskes G.
FEBS Lett. 297:135-138(1992) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
Tissue: Liver.
[2]"Monoclonal anti-FLAG antibodies react with a new isoform of rat Mg2+ dependent protein phosphatase beta."
Schafer K., Braun T.
Biochem. Biophys. Res. Commun. 207:708-714(1995) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 2).
Tissue: Brain.
[3]"Protein phosphatase 1B. Cloning and characterization of two major transcripts generated by alternative use of 3' exons."
Seroussi E., Shani N., Hayut A., Faier S., Ben-Meir D., Divinski I., Smorodinsky N.I., Lavi S.
Submitted (FEB-2000) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
S90449 mRNA. Translation: AAB21898.1.
S74572 mRNA. Translation: AAB33430.1.
AJ271837 mRNA. Translation: CAC28067.1.
PIRJC2524.
S20392.
RefSeqNP_149087.1. NM_033096.2.
UniGeneRn.4143.

3D structure databases

ProteinModelPortalP35815.
ModBaseSearch...
MobiDBSearch...

Proteomic databases

PaxDbP35815.
PRIDEP35815.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSRNOT00000048656; ENSRNOP00000048342; ENSRNOG00000030667. [P35815-1]
GeneID24667.
KEGGrno:24667.
UCSCRGD:3374. rat. [P35815-1]

Organism-specific databases

CTD5495.
RGD3374. Ppm1b.

Phylogenomic databases

eggNOGCOG0631.
GeneTreeENSGT00740000115384.
HOGENOMHOG000233895.
HOVERGENHBG053647.
KOK04461.

Gene expression databases

GenevestigatorP35815.

Family and domain databases

Gene3D1.10.10.430. 1 hit.
3.60.40.10. 1 hit.
InterProIPR001932. PP2C-like_dom.
IPR012911. PP2C_C.
IPR000222. PP2C_Mn2_Asp60_BS.
IPR015655. Protein_Pase_2C.
[Graphical view]
PANTHERPTHR13832. PTHR13832. 1 hit.
PfamPF00481. PP2C. 1 hit.
PF07830. PP2C_C. 1 hit.
[Graphical view]
SMARTSM00331. PP2C_SIG. 1 hit.
SM00332. PP2Cc. 1 hit.
[Graphical view]
SUPFAMSSF81601. SSF81601. 1 hit.
SSF81606. SSF81606. 1 hit.
PROSITEPS01032. PP2C. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio604030.
PROP35815.

Entry information

Entry namePPM1B_RAT
AccessionPrimary (citable) accession number: P35815
Secondary accession number(s): Q546R0, Q64046
Entry history
Integrated into UniProtKB/Swiss-Prot: June 1, 1994
Last sequence update: June 1, 1994
Last modified: March 19, 2014
This is version 110 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families