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Protein

Protein phosphatase 1B

Gene

Ppm1b

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at transcript leveli

Functioni

Enzyme with a broad specificity. Dephosphorylates PRKAA1 and PRKAA2. Inhibits TBK1-mediated antiviral signaling by dephosphorylating it at 'Ser-172'. Plays an important role in the termination of TNF-alpha-mediated NF-kappa-B activation through dephosphorylating and inactivating IKBKB/IKKB (By similarity).By similarity

Catalytic activityi

[a protein]-serine/threonine phosphate + H2O = [a protein]-serine/threonine + phosphate.

Cofactori

Mg2+By similarity, Mn2+By similarityNote: Binds 2 magnesium or manganese ions per subunit.By similarity

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi60 – 601Manganese 1By similarity
Metal bindingi60 – 601Manganese 2By similarity
Metal bindingi61 – 611Manganese 1; via carbonyl oxygenBy similarity
Metal bindingi243 – 2431Manganese 2By similarity
Metal bindingi286 – 2861Manganese 2By similarity

GO - Molecular functioni

  1. magnesium ion binding Source: InterPro
  2. manganese ion binding Source: InterPro
  3. protein serine/threonine phosphatase activity Source: InterPro

GO - Biological processi

  1. negative regulation of defense response to virus Source: UniProtKB
  2. negative regulation of I-kappaB kinase/NF-kappaB signaling Source: UniProtKB
  3. negative regulation of interferon-beta production Source: UniProtKB
  4. negative regulation of NF-kappaB import into nucleus Source: UniProtKB
  5. N-terminal protein myristoylation Source: UniProtKB
  6. protein dephosphorylation Source: UniProtKB
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Protein phosphatase

Keywords - Ligandi

Magnesium, Manganese, Metal-binding

Enzyme and pathway databases

ReactomeiREACT_198999. ISG15 antiviral mechanism.

Names & Taxonomyi

Protein namesi
Recommended name:
Protein phosphatase 1B (EC:3.1.3.16)
Alternative name(s):
Protein phosphatase 2C isoform beta
Short name:
PP2C-beta
Gene namesi
Name:Ppm1b
Synonyms:Pp2c2, Pppm1b
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
ProteomesiUP000002494: Chromosome 6

Organism-specific databases

RGDi3374. Ppm1b.

Subcellular locationi

Cytoplasmcytosol By similarity. Membrane By similarity
Note: Weakly associates at the membrane and N-myristoylation mediates the membrane localization.By similarity

GO - Cellular componenti

  1. cytosol Source: UniProtKB
  2. membrane Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methioninei1 – 11RemovedBy similarity
Chaini2 – 390389Protein phosphatase 1BPRO_0000057748Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Lipidationi2 – 21N-myristoyl glycineBy similarity
Cross-linki12 – 12Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ISG15)By similarity

Post-translational modificationi

Isgylation negatively regulates its activity.By similarity
N-myristoylation is essential for the recognition of its substrates for dephosphorylation.By similarity

Keywords - PTMi

Isopeptide bond, Lipoprotein, Myristate, Ubl conjugation

Proteomic databases

PaxDbiP35815.
PRIDEiP35815.

Expressioni

Gene expression databases

ExpressionAtlasiP35815. baseline and differential.
GenevestigatoriP35815.

Interactioni

Subunit structurei

Monomer. Interacts with PAK6. Interacts with the phosphorylated form of IKBKB/IKKB.By similarity

Structurei

3D structure databases

ProteinModelPortaliP35815.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the PP2C family.Curated

Phylogenomic databases

eggNOGiCOG0631.
GeneTreeiENSGT00740000115384.
HOGENOMiHOG000233895.
HOVERGENiHBG053647.
InParanoidiP35815.
KOiK04461.

Family and domain databases

Gene3Di1.10.10.430. 1 hit.
3.60.40.10. 1 hit.
InterProiIPR001932. PP2C-like_dom.
IPR012911. PP2C_C.
IPR000222. PP2C_Mn2_Asp60_BS.
IPR015655. Protein_Pase_2C.
[Graphical view]
PANTHERiPTHR13832. PTHR13832. 1 hit.
PfamiPF00481. PP2C. 1 hit.
PF07830. PP2C_C. 1 hit.
[Graphical view]
SMARTiSM00331. PP2C_SIG. 1 hit.
SM00332. PP2Cc. 1 hit.
[Graphical view]
SUPFAMiSSF81601. SSF81601. 1 hit.
SSF81606. SSF81606. 1 hit.
PROSITEiPS01032. PP2C. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 2 isoformsi produced by alternative splicing. Align

Note: Additional isoforms seem to exist. Isoforms appear to differ in their C-terminus.

Isoform 1 (identifier: P35815-1) [UniParc]FASTAAdd to Basket

Also known as: Beta-1

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MGAFLDKPKT EKHNAHGAGN GLRYGLSSMQ GWRVEMEDAH TAVVGIPHGL
60 70 80 90 100
EDWSFFAVYD GHAGSRVANY CSTHLLEHIT TNEDFRAADK SGFALEPSVE
110 120 130 140 150
NVKTGIRTGF LKIDEYMRNF SDLRNGMDRS GSTAVGVMIS PTHIYFINCG
160 170 180 190 200
DSRAVLCRNG QVCFSTQDHK PCNPMEKERI QNAGGSVMIQ RVNGSLAVSR
210 220 230 240 250
ALGDYDYKCV DGKGPTEQLV SPEPEVYEIL RAEEDEFVVL ACDGIWDVMS
260 270 280 290 300
NEELCEFVNS RLEVSDDLEN VCNWVVDTCL HKGSRDNMSI VLVCFANAPK
310 320 330 340 350
VSDEAVKRDL ELDKHLESRV EEIMQKSGEE GMPDLAHVMR ILSAENIPNL
360 370 380 390
PPGGGLAGKR NVIEAVYSRL NPNKDNDGGA GDLEDSLVAL
Length:390
Mass (Da):42,889
Last modified:June 1, 1994 - v1
Checksum:iD147615BC2FA140B
GO
Isoform 2 (identifier: P35815-2) [UniParc]FASTAAdd to Basket

Also known as: Beta-MPP

The sequence of this isoform differs from the canonical sequence as follows:
     379-390: GAGDLEDSLVAL → FYQPSTPYSDNVSYYEWQT

Show »
Length:397
Mass (Da):44,105
Checksum:iE4A7846C3FE787F6
GO

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei379 – 39012GAGDL…SLVAL → FYQPSTPYSDNVSYYEWQT in isoform 2. 1 PublicationVSP_005093Add
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
S90449 mRNA. Translation: AAB21898.1.
S74572 mRNA. Translation: AAB33430.1.
AJ271837 mRNA. Translation: CAC28067.1.
PIRiJC2524.
S20392.
RefSeqiNP_149087.1. NM_033096.2. [P35815-1]
UniGeneiRn.4143.

Genome annotation databases

EnsembliENSRNOT00000048656; ENSRNOP00000048342; ENSRNOG00000030667. [P35815-1]
GeneIDi24667.
KEGGirno:24667.
UCSCiRGD:3374. rat. [P35815-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
S90449 mRNA. Translation: AAB21898.1.
S74572 mRNA. Translation: AAB33430.1.
AJ271837 mRNA. Translation: CAC28067.1.
PIRiJC2524.
S20392.
RefSeqiNP_149087.1. NM_033096.2. [P35815-1]
UniGeneiRn.4143.

3D structure databases

ProteinModelPortaliP35815.
ModBaseiSearch...
MobiDBiSearch...

Proteomic databases

PaxDbiP35815.
PRIDEiP35815.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSRNOT00000048656; ENSRNOP00000048342; ENSRNOG00000030667. [P35815-1]
GeneIDi24667.
KEGGirno:24667.
UCSCiRGD:3374. rat. [P35815-1]

Organism-specific databases

CTDi5495.
RGDi3374. Ppm1b.

Phylogenomic databases

eggNOGiCOG0631.
GeneTreeiENSGT00740000115384.
HOGENOMiHOG000233895.
HOVERGENiHBG053647.
InParanoidiP35815.
KOiK04461.

Enzyme and pathway databases

ReactomeiREACT_198999. ISG15 antiviral mechanism.

Miscellaneous databases

NextBioi604030.
PROiP35815.

Gene expression databases

ExpressionAtlasiP35815. baseline and differential.
GenevestigatoriP35815.

Family and domain databases

Gene3Di1.10.10.430. 1 hit.
3.60.40.10. 1 hit.
InterProiIPR001932. PP2C-like_dom.
IPR012911. PP2C_C.
IPR000222. PP2C_Mn2_Asp60_BS.
IPR015655. Protein_Pase_2C.
[Graphical view]
PANTHERiPTHR13832. PTHR13832. 1 hit.
PfamiPF00481. PP2C. 1 hit.
PF07830. PP2C_C. 1 hit.
[Graphical view]
SMARTiSM00331. PP2C_SIG. 1 hit.
SM00332. PP2Cc. 1 hit.
[Graphical view]
SUPFAMiSSF81601. SSF81601. 1 hit.
SSF81606. SSF81606. 1 hit.
PROSITEiPS01032. PP2C. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Molecular cloning and primary structure of a protein phosphatase 2C isoform."
    Wenk J., Trompeter H.-I., Pettrich K.-G., Cohen P.T.W., Campbell D.G., Mieskes G.
    FEBS Lett. 297:135-138(1992) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
    Tissue: Liver.
  2. "Monoclonal anti-FLAG antibodies react with a new isoform of rat Mg2+ dependent protein phosphatase beta."
    Schafer K., Braun T.
    Biochem. Biophys. Res. Commun. 207:708-714(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 2).
    Tissue: Brain.
  3. "Protein phosphatase 1B. Cloning and characterization of two major transcripts generated by alternative use of 3' exons."
    Seroussi E., Shani N., Hayut A., Faier S., Ben-Meir D., Divinski I., Smorodinsky N.I., Lavi S.
    Submitted (FEB-2000) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).

Entry informationi

Entry nameiPPM1B_RAT
AccessioniPrimary (citable) accession number: P35815
Secondary accession number(s): Q546R0, Q64046
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 1, 1994
Last sequence update: June 1, 1994
Last modified: January 7, 2015
This is version 116 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.