P35814 (PPM1A_RABIT) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Protein phosphatase 1A
EC=3.1.3.16

Alternative name(s):
Protein phosphatase 2C isoform alpha
Short name=PP2C-alpha
Protein phosphatase IA

Gene names

Name:	PPM1A
Synonyms:	PPPM1A

Organism

Oryctolagus cuniculus (Rabbit) [Reference proteome]

Taxonomic identifier

9986 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Glires › Lagomorpha › Leporidae › Oryctolagus

Protein attributes

Sequence length	382 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Enzyme with a broad specificity. Negatively regulates TGF-beta signaling through dephosphorylating SMAD2 and SMAD3, resulting in their dissociation from SMAD4, nuclear export of the SMADs and termination of the TGF-beta-mediated signaling By similarity.
Catalytic activity	A phosphoprotein + H2O = a protein + phosphate.
Cofactor	Binds 2 magnesium or manganese ions per subunit.
Subunit structure	Monomer By similarity. Interacts with SMAD2; the interaction dephosphorylates SMAD2 in its C-terminal SXS motif resulting in disruption of the SMAD2/SMAD4 complex, SMAD2 nuclear export and termination of the TGF-beta-mediated signaling. Interacts with SMAD2; the interaction dephosphorylates SMAD2 in its C-terminal SXS motif resulting in disruption of the SMAD2/SMAD4 complex, SMAD2 nuclear export and termination of the TGF-beta-mediated signaling By similarity.
Subcellular location	Nucleus By similarity.
Sequence similarities	Belongs to the PP2C family.

Ontologies

Keywords
Cellular component	Nucleus
Ligand	Magnesium Manganese Metal-binding
Molecular function	Hydrolase Protein phosphatase
PTM	Lipoprotein Myristate Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	Wnt receptor signaling pathway Inferred from electronic annotation. Source: Compara negative regulation of SMAD protein complex assembly Inferred from electronic annotation. Source: Compara negative regulation of transforming growth factor beta receptor signaling pathway Inferred from electronic annotation. Source: Compara peptidyl-threonine dephosphorylation Inferred from electronic annotation. Source: Compara positive regulation of I-kappaB kinase/NF-kappaB cascade Inferred from sequence or structural similarity. Source: AgBase positive regulation of Wnt receptor signaling pathway Inferred from electronic annotation. Source: Compara positive regulation of transcription, DNA-dependent Inferred from electronic annotation. Source: Compara protein dephosphorylation Inferred from sequence or structural similarity. Source: AgBase
Cellular_component	nucleus Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	R-SMAD binding Inferred from sequence or structural similarity. Source: UniProtKB calmodulin-dependent protein phosphatase activity Inferred from electronic annotation. Source: Compara magnesium ion binding Inferred from electronic annotation. Source: InterPro manganese ion binding Inferred from electronic annotation. Source: InterPro phosphoprotein phosphatase activity Inferred from sequence or structural similarity. Source: AgBase signal transducer activity Inferred from sequence or structural similarity. Source: AgBase
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 382	382	Protein phosphatase 1A		PRO_0000057743
Sites
	Metal binding	60	1	Manganese 1 By similarity
	Metal binding	60	1	Manganese 2 By similarity
	Metal binding	61	1	Manganese 1; via carbonyl oxygen By similarity
	Metal binding	239	1	Manganese 2 By similarity
	Metal binding	282	1	Manganese 2 By similarity
Amino acid modifications
	Modified residue	375	1	Phosphoserine By similarity
	Modified residue	377	1	Phosphoserine By similarity
	Lipidation	2	1	N-myristoyl glycine By similarity

Sequences

	Sequence		Length	Mass (Da)
	P35814 [UniParc]. Last modified June 1, 1994. Version 1. Checksum: 46BCF1854FD1CA86	FASTA	382	42,462
	10 20 30 40 50 60 MGAFLDKPKM EKHNAQGQGN GLRYGLSSMQ GWRVEMEDAH TAVIGLPSGL ETWSFFAVYD 70 80 90 100 110 120 GHAGSQVAKY CCEHLLDHIT NNQDFKGSAG APSVENVKNG IRTGFLEIDE HMRVMSEKKH 130 140 150 160 170 180 GADRSGSTAV GVLISPQHTY FINCGDSRGL LCRNRKVHFF TQDHKPSNPL EKERIQNAGG 190 200 210 220 230 240 SVMIQRVNGS LAVSRALGDF DYKCVHGKGP TEQLVSPEPE VHDIERSEED DQFIILACDG 250 260 270 280 290 300 IWDVMGNEEL CDFVRSRLEV TDDLEKVCNE VVDTCLYKGS RDNMSVILIC FPNAPKVSPE 310 320 330 340 350 360 AVKKEAELDK YLECRVEEIL KKQGEGVPDL VHVMRTLASE NIPSLPPGGE LASKRNVIEA 370 380 VYNRLNPYKN DDTDSTSTDD MW

References

[1]	"Mammalian protein serine/threonine phosphatase 2C: cDNA cloning and comparative analysis of amino acid sequences." Mann D.J., Campbell D.G., McGowan C.H., Cohen P.T.W. Biochim. Biophys. Acta 1130:100-104(1992) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Tissue: Liver.

Cross-references

Sequence databases
EMBL GenBank DDBJ	S87757 mRNA. Translation: AAB21783.1.
PIR	S22422.
RefSeq	NP_001076167.1. NM_001082698.1.
UniGene	Ocu.3308.
3D structure databases
ProteinModelPortal	P35814.
SMR	P35814. Positions 2-368.
ModBase	Search...
Protein-protein interaction databases
STRING	9986.ENSOCUP00000008575.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	100009431.
Organism-specific databases
CTD	5494.
Phylogenomic databases
eggNOG	COG0631.
HOGENOM	HOG000233895.
HOVERGEN	HBG053647.
OrthoDB	EOG4GMTX1.
Family and domain databases
Gene3D	1.10.10.430. 1 hit. 3.60.40.10. 1 hit.
InterPro	IPR001932. PP2C-like. IPR012911. PP2C_C. IPR000222. PP2C_Mn2_Asp60_BS. IPR015655. Protein_Pase_2C. [Graphical view]
PANTHER	PTHR13832. PTHR13832. 1 hit.
Pfam	PF00481. PP2C. 1 hit. PF07830. PP2C_C. 1 hit. [Graphical view]
SMART	SM00331. PP2C_SIG. 1 hit. SM00332. PP2Cc. 1 hit. [Graphical view]
SUPFAM	SSF81606. PP2C-related. 1 hit. SSF81601. PP2C_C. 1 hit.
PROSITE	PS01032. PP2C. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

PPM1A_RABIT

Accession

Primary (citable) accession number: P35814

Entry history

Integrated into UniProtKB/Swiss-Prot:	June 1, 1994
Last sequence update:	June 1, 1994
Last modified:	April 3, 2013
This is version 91 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program