P35810 (GYRA_FIBSS) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
DNA gyrase subunit A
EC=5.99.1.3

Gene names

Name:	gyrA
Ordered Locus Names:	Fisuc_0363, FSU_0778

Organism

Fibrobacter succinogenes (strain ATCC 19169 / S85) [Complete proteome] [HAMAP]

Taxonomic identifier

59374 [NCBI]

Taxonomic lineage

Bacteria › Fibrobacteres › Fibrobacterales › Fibrobacteraceae › Fibrobacter

Protein attributes

Sequence length	903 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	DNA gyrase negatively supercoils closed circular double-stranded DNA in an ATP-dependent manner and also catalyzes the interconversion of other topological isomers of double-stranded DNA rings, including catenanes and knotted rings. HAMAP MF_01897
Catalytic activity	ATP-dependent breakage, passage and rejoining of double-stranded DNA. HAMAP MF_01897
Subunit structure	Made up of two chains. The A chain is responsible for DNA breakage and rejoining; the B chain catalyzes ATP hydrolysis. The enzyme forms an A2B2 tetramer.
Subcellular location	Cytoplasm Potential HAMAP MF_01897.
Sequence similarities	Belongs to the topoisomerase GyrA/ParC subunit family.
Sequence caution	The sequence ADL25539.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.

Ontologies

Keywords
Cellular component	Cytoplasm
Ligand	ATP-binding DNA-binding Nucleotide-binding
Molecular function	Isomerase Topoisomerase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	DNA topological change Inferred from electronic annotation. Source: InterPro regulation of transcription, DNA-dependent Inferred from electronic annotation. Source: InterPro
Cellular component	chromosome Inferred from electronic annotation. Source: InterPro cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW DNA topoisomerase (ATP-hydrolyzing) activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 903	903	DNA gyrase subunit A HAMAP MF_01897		PRO_0000145234
Sites
	Active site	124	1	O-(5'-phospho-DNA)-tyrosine intermediate By similarity

Sequences

	Sequence		Length	Mass (Da)
	P35810 [UniParc]. Last modified January 19, 2010. Version 2. Checksum: 68F9820762B8ED8D	FASTA	903	99,633
	10 20 30 40 50 60 MSEEMVPGSQ FKSLVEQDMQ DCYLRYSMSV IVARALPDAR DGFKPVHRRV MYSMHKLGVV 70 80 90 100 110 120 PNKGTVKSAR IVGDVIGKYH PHGDVAVYDT LARMAQDFSL RYPLVFGQGN FGSIDGDSPA 130 140 150 160 170 180 AMRYTEAKMN NLGALMLEDL EKETVDMGPN YDESLEEPLV LPSALPNMIV NGTSGIAVGM 190 200 210 220 230 240 ATNMAPHNLR EVGAAIHALA ENPDLTGEDL MEYVKGPDFP TGAIVCGRSG IREAYLTGHG 250 260 270 280 290 300 RVRVRARTEI ETDAKGKPRI IVTEIPYMVN KAELCKKIAD LVRDKRIDGI TDIRDESSRD 310 320 330 340 350 360 IRIVIELRRD AVGEVVLNNL FKYTQLQTTF SIYNLALVNN LPKLLTLKDL LQVYIDHRLD 370 380 390 400 410 420 VITRATQFDL KKAAARLHII EGLRIATQNI DEVVKIIRQS KTTEIAKQSL QDRFSLDEIQ 430 440 450 460 470 480 SQAIVDMRLA QLVGLNIEKL EAEYNELIAT VADLKDILEK RERRVAIMLQ KLDAVVDKYG 490 500 510 520 530 540 DERRTTIGEA IDDSDDEDLI AEEEQVITLS KEGYIRRLPI DTFKAQNRGG KGIIGAGLKD 550 560 570 580 590 600 EDNVEQIFTA STHSYLLVFT NKGRVYWTKV YRLPEGARNG KGRPIVNFVA LTEGEKVQAI 610 620 630 640 650 660 VPVRKFGGYF CLVFATKKGI INKMDLTLFS RPRKAGVNAI SLDEDDELVK VQLVGMSAEE 670 680 690 700 710 720 YEASKNASDD DSAEAVENAA EAQAAEAAIA EESESGDAED FANRPIPKDL LMIATKNGQA 730 740 750 760 770 780 VTFPISCFRA MGRGTHGVKG ITLAEGDEVI SLLWLKAGNK ILTITEKGYG KRSEPGSYRV 790 800 810 820 830 840 TRRGSKGVRN LNVTDKIGAA VFVESVADDY DLIITSKDGQ VIRIKAADIR LTGRNAQGVK 850 860 870 880 890 900 AITLRDGDVV KDATALPSVE DIEQDSADAK ETFDKVKGVE VDDDSVVKDD AEKQEIGPTE TEE

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Complete sequence of Fibrobacter succinogenes subsp. succinogenes S85." US DOE Joint Genome Institute Lucas S., Copeland A., Lapidus A., Glavina del Rio T., Tice H., Bruce D., Goodwin L., Pitluck S., Chertkov O., Detter J.C., Han C., Tapia R., Larimer F., Land M., Hauser L., Kyrpides N., Mikhailova N., Weimer P.J. , Stevenson D.M., Boyum J., Brumm P.I., Mead D. Submitted (OCT-2009) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 19169 / S85.
[2]	"Complete sequence of Fibrobacter succinogenes subsp. succinogenes S85." Durkin A.S., Nelson K.E., Morrison M., Forsberg C.W., Wilson D.B., Russell J.B., Cann I.K.O., Mackie R.I., White B.A. Submitted (AUG-2010) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 19169 / S85.
[3]	"The xynC gene from Fibrobacter succinogenes S85 codes for a xylanase with two similar catalytic domains." Paradis F.W., Zhu H., Krell P.J., Phillips J.P., Forsberg C.W. J. Bacteriol. 175:7666-7672(1993) [PubMed: 8244936] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 709-903.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP001792 Genomic DNA. Translation: ACX73975.1. CP002158 Genomic DNA. Translation: ADL25539.1. Different initiation. U01037 Genomic DNA. Translation: AAA21849.1.
PIR	A53295.
RefSeq	YP_003248457.1. NC_013410.1.
3D structure databases
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	8520949.
GenomeReviews	Gene locus Fisuc_0363 in contig CP001792_GR. Gene locus FSU_0778 in contig CP002158_GR.
KEGG	fsu:Fisuc_0363.
PATRIC	32140501. VBIFibSuc28982_0355.
Organism-specific databases
CMR	Search...
Phylogenomic databases
OMA	TGRGRIY.
Family and domain databases
HAMAP	MF_01897. GyrA. [Tree]
InterPro	IPR005743. GyrA. IPR006691. GyrA/parC_pinwhl. IPR002205. Topo_IIA_A/C. IPR013758. Topo_IIA_A/C_ab. IPR013757. Topo_IIA_A_a. IPR013760. Topo_IIA_cen. [Graphical view]
Gene3D	G3DSA:3.90.199.10. Topo_IIA_A/C_ab. 1 hit. G3DSA:1.10.268.10. Topo_IIA_A_a. 1 hit.
KO	K02469.
Pfam	PF03989. DNA_gyraseA_C. 6 hits. PF00521. DNA_topoisoIV. 1 hit. [Graphical view]
SMART	SM00434. TOP4c. 1 hit. [Graphical view]
SUPFAM	SSF56719. Topo_IIA_cen. 1 hit.
TIGRFAMs	TIGR01063. GyrA. 1 hit.
ProtoNet	Search...

Entry information

Entry name

GYRA_FIBSS

Accession

Primary (citable) accession number: P35810
Secondary accession number(s): C9RKP7, D9S813

Entry history

Integrated into UniProtKB/Swiss-Prot:	June 1, 1994
Last sequence update:	January 19, 2010
Last modified:	January 25, 2012
This is version 54 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program