P35809 (XYNA_SCHCO) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Endo-1,4-beta-xylanase A
Short name=Xylanase A
EC=3.2.1.8

Alternative name(s):
1,4-beta-D-xylan xylanohydrolase A

Gene names

Name:

XYNA

Organism

Schizophyllum commune (Split gill fungus)

Taxonomic identifier

5334 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Fungi › Dikarya › Basidiomycota › Agaricomycotina › Agaricomycetes › Agaricomycetidae › Agaricales › Schizophyllaceae › Schizophyllum

Protein attributes

Sequence length	197 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Hydrolyzes xylans into xylobiose and xylose.
Catalytic activity	Endohydrolysis of (1->4)-beta-D-xylosidic linkages in xylans.
Pathway	Glycan degradation; xylan degradation.
Subcellular location	Secreted.
Sequence similarities	Belongs to the glycosyl hydrolase 11 (cellulase G) family.
Biophysicochemical properties	pH dependence: Active over a very broad pH range.

Ontologies

Keywords
Biological process	Xylan degradation
Cellular component	Secreted
Molecular function	Glycosidase Hydrolase
PTM	Disulfide bond
Technical term	Direct protein sequencing
Gene Ontology (GO)
Biological_process	xylan catabolic process Inferred from electronic annotation. Source: UniProtKB-UniPathway
Cellular_component	extracellular region Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	endo-1,4-beta-xylanase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 197	197	Endo-1,4-beta-xylanase A		PRO_0000184071
Sites
	Active site	87	1	Nucleophile Probable
	Active site	184	1	Proton donor By similarity
Amino acid modifications
	Disulfide bond	111 ↔ 160		Ref.2

Sequences

	Sequence		Length	Mass (Da)
	P35809 [UniParc]. Last modified June 1, 1994. Version 1. Checksum: 42C8074E67C1FBE9	FASTA	197	20,979
	10 20 30 40 50 60 SGTPSSTGTD GGYYYSWWTD GAGDATYQNN GGGSYTLTWS GNNGNLVGGK GWNPGAASRS 70 80 90 100 110 120 ISYSGTYQPN GNSYLSVYGW TRSSLIEYYI VESYGSYDPS SAASHKGSVT CNGATYDILS 130 140 150 160 170 180 TWRYNAPSID GTQTFEQFWS VRNPKKAPGG SISGTVDVQC HFDAWKGLGM NLGSEHNYQI 190 VATEGYQSSG TATITVT

References

[1]	Yaguchi M., Roy C., Ujiie M., Watson D.C., Wakarchuk W. (In) Visser J., Beldman G., Kusters-van Someren M.A., Voragen A.G.J. (eds.); Xylans and xylanases, pp.149-154, Elsevier, Amsterdam (1992) Cited for: PROTEIN SEQUENCE. Strain: ATCC 38548 / Delmar / 13 / IHEM 5263.
[2]	"Amino acid sequence and thermostability of xylanase A from Schizophyllum commune." Oku T., Roy C., Watson D.C., Wakarchuk W., Campbell R., Yaguchi M., Jurasek L., Paice M.G. FEBS Lett. 334:296-300(1993) [PubMed] [Europe PMC] [Abstract] Cited for: PROTEIN SEQUENCE, DISULFIDE BONDS. Strain: ATCC 38548 / Delmar / 13 / IHEM 5263.
[3]	"Identification of a glutamate residue at the active site of xylanase A from Schizophyllum commune." Bray M.R., Clarke A.J. Eur. J. Biochem. 219:821-827(1994) [PubMed] [Europe PMC] [Abstract] Cited for: PARTIAL PROTEIN SEQUENCE, ACTIVE SITE GLU-87. Strain: ATCC 38548 / Delmar / 13 / IHEM 5263.

Cross-references

Sequence databases
PIR	A44597.
3D structure databases
ProteinModelPortal	P35809.
SMR	P35809. Positions 3-196.
ModBase	Search...
Protein family/group databases
CAZy	GH11. Glycoside Hydrolase Family 11.
mycoCLAP	XYN11A_SCHCO.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Enzyme and pathway databases
UniPathway	UPA00114.
Family and domain databases
Gene3D	2.60.120.180. 1 hit.
InterPro	IPR008985. ConA-like_lec_gl_sf. IPR001137. Glyco_hydro_11. IPR013319. Glyco_hydro_11/12. IPR018208. Glyco_hydro_11_AS. [Graphical view]
Pfam	PF00457. Glyco_hydro_11. 1 hit. [Graphical view]
PRINTS	PR00911. GLHYDRLASE11.
SUPFAM	SSF49899. ConA_like_lec_gl. 1 hit.
PROSITE	PS00776. GLYCOSYL_HYDROL_F11_1. 1 hit. PS00777. GLYCOSYL_HYDROL_F11_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

XYNA_SCHCO

Accession

Primary (citable) accession number: P35809

Entry history

Integrated into UniProtKB/Swiss-Prot:	June 1, 1994
Last sequence update:	June 1, 1994
Last modified:	April 3, 2013
This is version 73 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program