P35802 (GPM6A_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Neuronal membrane glycoprotein M6-a
Short name=M6a

Gene names

Name:	Gpm6a
Synonyms:	M6a

Organism

Mus musculus (Mouse) [Reference proteome]

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus › Mus

Protein attributes

Sequence length	278 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Involved in neuronal differentiation, including differentiation and migration of neuronal stem cells. Plays a role in neuronal plasticity and is involved in neurite and filopodia outgrowth, filopodia motility and probably synapse formation. Gpm6a-induced filopodia formation involves mitogen-activated protein kinase (MAPK) and Src signaling pathways. Conflictingly, Ref.7 reports that induced cellular protrusions are simple membrane-wrapped tubules without actin or tubulin-based cytoskeletons and with Gpm6a gliding along membrane edges indicative for a function in actin-indepenedent membrane deformation. May be involved in neuronal NGF-dependent Ca2+ influx. May be involved in regulation of endocytosis and intracellular trafficking of G-protein-coupled receptors (GPCRs); enhances internalization and recycling of mu-type opioid receptor. Ref.4 Ref.5 Ref.7
Subcellular location	Cell membrane; Multi-pass membrane protein. Cell projection › axon. Cell projection › dendritic spine By similarity. Cell projection › filopodium By similarity. Note: Localizes to cholesterol-rich lipid rafts of the plasma membrane of hippocampal neurons. Localized to plasma membrane of cell bodies and neurites of hippocampal neurons. Localized in membrane protrusions (filopodia and spines) of primary hippocampal neurons By similarity. Localized to the growth cone edge membrane of elongating axons. Ref.6
Tissue specificity	Widely expressed in the CNS. Found especially in the granule cell layer of the cerebellum but not in the molecular layer or white matter. Expressed in the immature embryonic retina including the nerve fiber layer (NFL), inner plexiform layer (IPL), and outer plexiform layer (OPL). Weakly expressed in processes of Mueller glia cells. Ref.4
Developmental stage	First detected in presumptive postmitotic neurons in the developing neural tube at embryonic day 9. Expressed in E14 retinas, and expression continued after birth with a slight decrease between P12 and P15. In the E14 retina is mainly and strongly expressed in the NFL. In P1 and P5 retinas strong expression is confined to the IPL and also in NFL. At P10 and in the adult retina strong expression is detected in the IPL, and weak expression in NFL, OPL, and inner nuclear layer. Is expressed on postmitotic mature neurons. Ref.4
Sequence similarities	Belongs to the myelin proteolipid protein family.

Ontologies

Keywords
Biological process	Neurogenesis
Cellular component	Cell membrane Cell projection Membrane
Domain	Transmembrane Transmembrane helix
PTM	Disulfide bond Glycoprotein
Technical term	Complete proteome Direct protein sequencing Reference proteome
Gene Ontology (GO)
Biological_process	neural retina development Inferred from direct assay Ref.4. Source: UniProtKB neuron migration Inferred from sequence or structural similarity. Source: UniProtKB neuron projection morphogenesis Inferred from direct assay Ref.4. Source: UniProtKB positive regulation of filopodium assembly Inferred from sequence or structural similarity. Source: UniProtKB stem cell differentiation Inferred from sequence or structural similarity. Source: UniProtKB synapse assembly Inferred from sequence or structural similarity. Source: UniProtKB
Cellular_component	axonal growth cone Inferred from direct assay Ref.6. Source: UniProtKB cell surface Inferred from electronic annotation. Source: Compara dendritic spine Inferred from electronic annotation. Source: UniProtKB-SubCell filopodium Inferred from sequence or structural similarity. Source: UniProtKB integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW neuronal cell body Inferred from sequence or structural similarity. Source: UniProtKB plasma membrane Inferred from sequence or structural similarity. Source: UniProtKB
Molecular_function	calcium channel activity Inferred from electronic annotation. Source: Compara
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 278	278	Neuronal membrane glycoprotein M6-a		PRO_0000159019
Regions
	Topological domain	1 – 22	22	Cytoplasmic Potential
	Transmembrane	23 – 43	21	Helical; Potential
	Topological domain	44 – 84	41	Extracellular Potential
	Transmembrane	85 – 105	21	Helical; Potential
	Topological domain	106 – 127	22	Cytoplasmic Potential
	Transmembrane	128 – 148	21	Helical; Potential
	Topological domain	149 – 213	65	Extracellular By similarity
	Transmembrane	214 – 234	21	Helical; Potential
	Topological domain	235 – 278	44	Cytoplasmic Potential
Amino acid modifications
	Glycosylation	164	1	N-linked (GlcNAc...) Potential
	Glycosylation	208	1	N-linked (GlcNAc...) Potential
	Disulfide bond	174 ↔ 192		By similarity

Sequences

	Sequence		Length	Mass (Da)
	P35802 [UniParc]. Last modified June 1, 1994. Version 1. Checksum: CEE0688873E786CE	FASTA	278	31,149
	10 20 30 40 50 60 MEENMEEGQT QKGCFECCIK CLGGIPYASL IATILLYAGV ALFCGCGHEA LSGTVNILQT 70 80 90 100 110 120 YFELARTAGD TLDVFTMIDI FKYVIYGIAA AFFVYGILLM VEGFFTTGAI KDLYGDFKIT 130 140 150 160 170 180 TCGRCVSAWF IMLTYLFMLA WLGVTAFTSL PVYMYFNVWT ICRNTTLVEG ANLCLDLRQF 190 200 210 220 230 240 GIVTIGEEKK ICTASENFLR MCESTELNMT FHLFIVALAG AGAAVIAMVH YLMVLSANWA 250 260 270 YVKDACRMQK YEDIKSKEEQ ELHDIHSTRS KERLNAYT

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Molecular cloning of M6: identification of a PLP/DM20 gene family." Yan Y., Lagenaur C., Narayanan V. Neuron 11:423-431(1993) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], PROTEIN SEQUENCE OF 67-78. Tissue: Brain.
[2]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: C57BL/6. Tissue: Brain, Eye and Retina.
[3]	Lubec G., Kang S.U. Submitted (APR-2007) to UniProtKB Cited for: PROTEIN SEQUENCE OF 13-20; 67-82; 112-118; 179-200 AND 256-269, MASS SPECTROMETRY. Strain: C57BL/6. Tissue: Brain.
[4]	"M6a is expressed in the murine neural retina and regulates neurite extension." Zhao J., Iida A., Ouchi Y., Satoh S., Watanabe S. Mol. Vis. 14:1623-1630(2008) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION, DEVELOPMENTAL STAGE, TISSUE SPECIFICITY.
[5]	"Inhibition of mouse GPM6A expression leads to decreased differentiation of neurons derived from mouse embryonic stem cells." Michibata H., Okuno T., Konishi N., Wakimoto K., Kyono K., Aoki K., Kondo Y., Takata K., Kitamura Y., Taniguchi T. Stem Cells Dev. 17:641-651(2008) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION.
[6]	"Induction of axon growth arrest without growth cone collapse through the N-terminal region of four-transmembrane glycoprotein M6a." Sato Y., Mita S., Fukushima N., Fujisawa H., Saga Y., Hirata T. Dev. Neurobiol. 71:733-746(2011) [PubMed] [Europe PMC] [Abstract] Cited for: SUBCELLULAR LOCATION.
[7]	"Actin-independent behavior and membrane deformation exhibited by the four-transmembrane protein M6a." Sato Y., Watanabe N., Fukushima N., Mita S., Hirata T. PLoS ONE 6:E26702-E26702(2011) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	S65735 mRNA. Translation: AAB28350.1. BC024759 mRNA. Translation: AAH24759.1. BC029683 mRNA. Translation: AAH29683.1. BC029711 mRNA. Translation: AAH29711.1. BC033357 mRNA. Translation: AAH33357.1. BC033394 mRNA. Translation: AAH33394.1. BC043130 mRNA. Translation: AAH43130.1.
IPI	IPI00122974.
RefSeq	NP_001240683.1. NM_001253754.1. NP_001240685.1. NM_001253756.1. NP_705809.1. NM_153581.6.
UniGene	Mm.241700.
3D structure databases
ProteinModelPortal	P35802.
ModBase	Search...
Protein-protein interaction databases
IntAct	P35802. 1 interaction.
MINT	MINT-4096535.
STRING	10090.ENSMUSP00000033915.
Protein family/group databases
TCDB	9.B.38.1.1. myelin proteolipid protein (MPLP) family.
PTM databases
PhosphoSite	P35802.
Proteomic databases
PaxDb	P35802.
PRIDE	P35802.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENSMUST00000033915; ENSMUSP00000033915; ENSMUSG00000031517.
GeneID	234267.
KEGG	mmu:234267.
UCSC	uc009lsl.2. mouse.
Organism-specific databases
CTD	2823.
MGI	MGI:107671. Gpm6a.
Phylogenomic databases
eggNOG	NOG322245.
HOGENOM	HOG000231338.
HOVERGEN	HBG000096.
InParanoid	P35802.
OMA	NELEYSA.
OrthoDB	EOG4KSPKC.
Gene expression databases
ArrayExpress	P35802.
Bgee	P35802.
CleanEx	MM_GPM6A.
Genevestigator	P35802.
GermOnline	ENSMUSG00000031517. Mus musculus.
Family and domain databases
InterPro	IPR001614. Myelin_PLP. IPR018237. Myelin_PLP_CS. [Graphical view]
PANTHER	PTHR11683. PTHR11683. 1 hit.
Pfam	PF01275. Myelin_PLP. 1 hit. [Graphical view]
PRINTS	PR00214. MYELINPLP.
SMART	SM00002. PLP. 1 hit. [Graphical view]
PROSITE	PS00575. MYELIN_PLP_1. 1 hit. PS01004. MYELIN_PLP_2. 1 hit. [Graphical view]
ProtoNet	Search...
Other
ChiTaRS	GPM6A. mouse.
NextBio	382077.
SOURCE	Search...

Entry information

Entry name

GPM6A_MOUSE

Accession

Primary (citable) accession number: P35802

Entry history

Integrated into UniProtKB/Swiss-Prot:	June 1, 1994
Last sequence update:	June 1, 1994
Last modified:	May 29, 2013
This is version 101 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program