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P35802

- GPM6A_MOUSE

UniProt

P35802 - GPM6A_MOUSE

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Protein

Neuronal membrane glycoprotein M6-a

Gene

Gpm6a

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli

Functioni

Involved in neuronal differentiation, including differentiation and migration of neuronal stem cells. Plays a role in neuronal plasticity and is involved in neurite and filopodia outgrowth, filopodia motility and probably synapse formation. Gpm6a-induced filopodia formation involves mitogen-activated protein kinase (MAPK) and Src signaling pathways. Conflictingly, PubMed:22162747 reports that induced cellular protrusions are simple membrane-wrapped tubules without actin or tubulin-based cytoskeletons and with Gpm6a gliding along membrane edges indicative for a function in actin-independent membrane deformation. May be involved in neuronal NGF-dependent Ca2+ influx. May be involved in regulation of endocytosis and intracellular trafficking of G-protein-coupled receptors (GPCRs); enhances internalization and recycling of mu-type opioid receptor.3 Publications

GO - Molecular functioni

  1. calcium channel activity Source: Ensembl

GO - Biological processi

  1. neural retina development Source: UniProtKB
  2. neuron migration Source: UniProtKB
  3. neuron projection morphogenesis Source: UniProtKB
  4. positive regulation of filopodium assembly Source: UniProtKB
  5. stem cell differentiation Source: UniProtKB
  6. synapse assembly Source: UniProtKB
Complete GO annotation...

Keywords - Biological processi

Neurogenesis

Protein family/group databases

TCDBi9.B.38.1.1. the myelin proteolipid protein (mplp) family.

Names & Taxonomyi

Protein namesi
Recommended name:
Neuronal membrane glycoprotein M6-a
Short name:
M6a
Gene namesi
Name:Gpm6a
Synonyms:M6a
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589: Chromosome 8

Organism-specific databases

MGIiMGI:107671. Gpm6a.

Subcellular locationi

Cell membrane 1 Publication; Multi-pass membrane protein 1 Publication. Cell projectionaxon 1 Publication. Cell projectiondendritic spine By similarity. Cell projectionfilopodium By similarity
Note: Localizes to cholesterol-rich lipid rafts of the plasma membrane of hippocampal neurons. Localized to plasma membrane of cell bodies and neurites of hippocampal neurons. Localized in membrane protrusions (filopodia and spines) of primary hippocampal neurons (By similarity). Localized to the growth cone edge membrane of elongating axons.By similarity

GO - Cellular componenti

  1. axonal growth cone Source: UniProtKB
  2. extracellular vesicular exosome Source: Ensembl
  3. filopodium Source: UniProtKB
  4. integral component of membrane Source: UniProtKB-KW
  5. neuronal cell body Source: UniProtKB
  6. neuron projection Source: UniProtKB
  7. plasma membrane Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Cell projection, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 278278Neuronal membrane glycoprotein M6-aPRO_0000159019Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei1 – 11N-acetylmethionineBy similarity
Glycosylationi164 – 1641N-linked (GlcNAc...)Sequence Analysis
Disulfide bondi174 ↔ 192By similarity
Glycosylationi208 – 2081N-linked (GlcNAc...)Sequence Analysis

Keywords - PTMi

Acetylation, Disulfide bond, Glycoprotein

Proteomic databases

MaxQBiP35802.
PaxDbiP35802.
PRIDEiP35802.

PTM databases

PhosphoSiteiP35802.

Expressioni

Tissue specificityi

Widely expressed in the CNS. Found especially in the granule cell layer of the cerebellum but not in the molecular layer or white matter. Expressed in the immature embryonic retina including the nerve fiber layer (NFL), inner plexiform layer (IPL), and outer plexiform layer (OPL). Weakly expressed in processes of Mueller glia cells.1 Publication

Developmental stagei

First detected in presumptive postmitotic neurons in the developing neural tube at embryonic day 9. Expressed in E14 retinas, and expression continued after birth with a slight decrease between P12 and P15. In the E14 retina is mainly and strongly expressed in the NFL. In P1 and P5 retinas strong expression is confined to the IPL and also in NFL. At P10 and in the adult retina strong expression is detected in the IPL, and weak expression in NFL, OPL, and inner nuclear layer. Is expressed on postmitotic mature neurons.1 Publication

Gene expression databases

BgeeiP35802.
CleanExiMM_GPM6A.
ExpressionAtlasiP35802. baseline and differential.
GenevestigatoriP35802.

Interactioni

Protein-protein interaction databases

IntActiP35802. 5 interactions.
MINTiMINT-4096535.
STRINGi10090.ENSMUSP00000033915.

Structurei

3D structure databases

ProteinModelPortaliP35802.
ModBaseiSearch...
MobiDBiSearch...

Topological domain

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 2222CytoplasmicSequence AnalysisAdd
BLAST
Topological domaini44 – 8441ExtracellularSequence AnalysisAdd
BLAST
Topological domaini106 – 12722CytoplasmicSequence AnalysisAdd
BLAST
Topological domaini149 – 21365ExtracellularBy similarityAdd
BLAST
Topological domaini235 – 27844CytoplasmicSequence AnalysisAdd
BLAST

Transmembrane

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transmembranei23 – 4321HelicalSequence AnalysisAdd
BLAST
Transmembranei85 – 10521HelicalSequence AnalysisAdd
BLAST
Transmembranei128 – 14821HelicalSequence AnalysisAdd
BLAST
Transmembranei214 – 23421HelicalSequence AnalysisAdd
BLAST

Family & Domainsi

Sequence similaritiesi

Belongs to the myelin proteolipid protein family.Curated

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiNOG322245.
HOGENOMiHOG000231338.
HOVERGENiHBG000096.
InParanoidiP35802.
OMAiEEKKVCT.
OrthoDBiEOG7X3QRG.
PhylomeDBiP35802.
TreeFamiTF315162.

Family and domain databases

InterProiIPR001614. Myelin_PLP.
IPR018237. Myelin_PLP_CS.
[Graphical view]
PANTHERiPTHR11683. PTHR11683. 1 hit.
PfamiPF01275. Myelin_PLP. 1 hit.
[Graphical view]
PRINTSiPR00214. MYELINPLP.
SMARTiSM00002. PLP. 1 hit.
[Graphical view]
PROSITEiPS00575. MYELIN_PLP_1. 1 hit.
PS01004. MYELIN_PLP_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P35802-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MEENMEEGQT QKGCFECCIK CLGGIPYASL IATILLYAGV ALFCGCGHEA
60 70 80 90 100
LSGTVNILQT YFELARTAGD TLDVFTMIDI FKYVIYGIAA AFFVYGILLM
110 120 130 140 150
VEGFFTTGAI KDLYGDFKIT TCGRCVSAWF IMLTYLFMLA WLGVTAFTSL
160 170 180 190 200
PVYMYFNVWT ICRNTTLVEG ANLCLDLRQF GIVTIGEEKK ICTASENFLR
210 220 230 240 250
MCESTELNMT FHLFIVALAG AGAAVIAMVH YLMVLSANWA YVKDACRMQK
260 270
YEDIKSKEEQ ELHDIHSTRS KERLNAYT
Length:278
Mass (Da):31,149
Last modified:June 1, 1994 - v1
Checksum:iCEE0688873E786CE
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
S65735 mRNA. Translation: AAB28350.1.
BC024759 mRNA. Translation: AAH24759.1.
BC029683 mRNA. Translation: AAH29683.1.
BC029711 mRNA. Translation: AAH29711.1.
BC033357 mRNA. Translation: AAH33357.1.
BC033394 mRNA. Translation: AAH33394.1.
BC043130 mRNA. Translation: AAH43130.1.
CCDSiCCDS22310.1.
RefSeqiNP_001240683.1. NM_001253754.1.
NP_001240685.1. NM_001253756.1.
NP_705809.1. NM_153581.6.
UniGeneiMm.241700.

Genome annotation databases

EnsembliENSMUST00000033915; ENSMUSP00000033915; ENSMUSG00000031517.
GeneIDi234267.
KEGGimmu:234267.
UCSCiuc009lsl.2. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
S65735 mRNA. Translation: AAB28350.1 .
BC024759 mRNA. Translation: AAH24759.1 .
BC029683 mRNA. Translation: AAH29683.1 .
BC029711 mRNA. Translation: AAH29711.1 .
BC033357 mRNA. Translation: AAH33357.1 .
BC033394 mRNA. Translation: AAH33394.1 .
BC043130 mRNA. Translation: AAH43130.1 .
CCDSi CCDS22310.1.
RefSeqi NP_001240683.1. NM_001253754.1.
NP_001240685.1. NM_001253756.1.
NP_705809.1. NM_153581.6.
UniGenei Mm.241700.

3D structure databases

ProteinModelPortali P35802.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

IntActi P35802. 5 interactions.
MINTi MINT-4096535.
STRINGi 10090.ENSMUSP00000033915.

Protein family/group databases

TCDBi 9.B.38.1.1. the myelin proteolipid protein (mplp) family.

PTM databases

PhosphoSitei P35802.

Proteomic databases

MaxQBi P35802.
PaxDbi P35802.
PRIDEi P35802.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENSMUST00000033915 ; ENSMUSP00000033915 ; ENSMUSG00000031517 .
GeneIDi 234267.
KEGGi mmu:234267.
UCSCi uc009lsl.2. mouse.

Organism-specific databases

CTDi 2823.
MGIi MGI:107671. Gpm6a.

Phylogenomic databases

eggNOGi NOG322245.
HOGENOMi HOG000231338.
HOVERGENi HBG000096.
InParanoidi P35802.
OMAi EEKKVCT.
OrthoDBi EOG7X3QRG.
PhylomeDBi P35802.
TreeFami TF315162.

Miscellaneous databases

ChiTaRSi GPM6A. mouse.
NextBioi 382077.
PROi P35802.
SOURCEi Search...

Gene expression databases

Bgeei P35802.
CleanExi MM_GPM6A.
ExpressionAtlasi P35802. baseline and differential.
Genevestigatori P35802.

Family and domain databases

InterProi IPR001614. Myelin_PLP.
IPR018237. Myelin_PLP_CS.
[Graphical view ]
PANTHERi PTHR11683. PTHR11683. 1 hit.
Pfami PF01275. Myelin_PLP. 1 hit.
[Graphical view ]
PRINTSi PR00214. MYELINPLP.
SMARTi SM00002. PLP. 1 hit.
[Graphical view ]
PROSITEi PS00575. MYELIN_PLP_1. 1 hit.
PS01004. MYELIN_PLP_2. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Molecular cloning of M6: identification of a PLP/DM20 gene family."
    Yan Y., Lagenaur C., Narayanan V.
    Neuron 11:423-431(1993) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], PROTEIN SEQUENCE OF 67-78.
    Tissue: Brain.
  2. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: C57BL/6.
    Tissue: Brain, Eye and Retina.
  3. Lubec G., Kang S.U.
    Submitted (APR-2007) to UniProtKB
    Cited for: PROTEIN SEQUENCE OF 13-20; 67-82; 112-118; 179-200 AND 256-269, IDENTIFICATION BY MASS SPECTROMETRY.
    Strain: C57BL/6.
    Tissue: Brain.
  4. "M6a is expressed in the murine neural retina and regulates neurite extension."
    Zhao J., Iida A., Ouchi Y., Satoh S., Watanabe S.
    Mol. Vis. 14:1623-1630(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, DEVELOPMENTAL STAGE, TISSUE SPECIFICITY.
  5. "Inhibition of mouse GPM6A expression leads to decreased differentiation of neurons derived from mouse embryonic stem cells."
    Michibata H., Okuno T., Konishi N., Wakimoto K., Kyono K., Aoki K., Kondo Y., Takata K., Kitamura Y., Taniguchi T.
    Stem Cells Dev. 17:641-651(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  6. "Induction of axon growth arrest without growth cone collapse through the N-terminal region of four-transmembrane glycoprotein M6a."
    Sato Y., Mita S., Fukushima N., Fujisawa H., Saga Y., Hirata T.
    Dev. Neurobiol. 71:733-746(2011) [PubMed] [Europe PMC] [Abstract]
    Cited for: SUBCELLULAR LOCATION.
  7. "Actin-independent behavior and membrane deformation exhibited by the four-transmembrane protein M6a."
    Sato Y., Watanabe N., Fukushima N., Mita S., Hirata T.
    PLoS ONE 6:E26702-E26702(2011) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.

Entry informationi

Entry nameiGPM6A_MOUSE
AccessioniPrimary (citable) accession number: P35802
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 1, 1994
Last sequence update: June 1, 1994
Last modified: October 29, 2014
This is version 113 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3