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P35802 (GPM6A_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 111. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Neuronal membrane glycoprotein M6-a

Short name=M6a
Gene names
Name:Gpm6a
Synonyms:M6a
OrganismMus musculus (Mouse) [Reference proteome]
Taxonomic identifier10090 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus

Protein attributes

Sequence length278 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Involved in neuronal differentiation, including differentiation and migration of neuronal stem cells. Plays a role in neuronal plasticity and is involved in neurite and filopodia outgrowth, filopodia motility and probably synapse formation. Gpm6a-induced filopodia formation involves mitogen-activated protein kinase (MAPK) and Src signaling pathways. Conflictingly, Ref.7 reports that induced cellular protrusions are simple membrane-wrapped tubules without actin or tubulin-based cytoskeletons and with Gpm6a gliding along membrane edges indicative for a function in actin-independent membrane deformation. May be involved in neuronal NGF-dependent Ca2+ influx. May be involved in regulation of endocytosis and intracellular trafficking of G-protein-coupled receptors (GPCRs); enhances internalization and recycling of mu-type opioid receptor. Ref.4 Ref.5 Ref.7

Subcellular location

Cell membrane; Multi-pass membrane protein. Cell projectionaxon. Cell projectiondendritic spine By similarity. Cell projectionfilopodium By similarity. Note: Localizes to cholesterol-rich lipid rafts of the plasma membrane of hippocampal neurons. Localized to plasma membrane of cell bodies and neurites of hippocampal neurons. Localized in membrane protrusions (filopodia and spines) of primary hippocampal neurons By similarity. Localized to the growth cone edge membrane of elongating axons. Ref.6

Tissue specificity

Widely expressed in the CNS. Found especially in the granule cell layer of the cerebellum but not in the molecular layer or white matter. Expressed in the immature embryonic retina including the nerve fiber layer (NFL), inner plexiform layer (IPL), and outer plexiform layer (OPL). Weakly expressed in processes of Mueller glia cells. Ref.4

Developmental stage

First detected in presumptive postmitotic neurons in the developing neural tube at embryonic day 9. Expressed in E14 retinas, and expression continued after birth with a slight decrease between P12 and P15. In the E14 retina is mainly and strongly expressed in the NFL. In P1 and P5 retinas strong expression is confined to the IPL and also in NFL. At P10 and in the adult retina strong expression is detected in the IPL, and weak expression in NFL, OPL, and inner nuclear layer. Is expressed on postmitotic mature neurons. Ref.4

Sequence similarities

Belongs to the myelin proteolipid protein family.

Ontologies

Keywords
   Biological processNeurogenesis
   Cellular componentCell membrane
Cell projection
Membrane
   DomainTransmembrane
Transmembrane helix
   PTMAcetylation
Disulfide bond
Glycoprotein
   Technical termComplete proteome
Direct protein sequencing
Reference proteome
Gene Ontology (GO)
   Biological_processneural retina development

Inferred from direct assay Ref.4. Source: UniProtKB

neuron migration

Inferred from sequence or structural similarity. Source: UniProtKB

neuron projection morphogenesis

Inferred from direct assay Ref.4. Source: UniProtKB

positive regulation of filopodium assembly

Inferred from sequence or structural similarity. Source: UniProtKB

stem cell differentiation

Inferred from sequence or structural similarity. Source: UniProtKB

synapse assembly

Inferred from sequence or structural similarity. Source: UniProtKB

   Cellular_componentaxonal growth cone

Inferred from direct assay Ref.6. Source: UniProtKB

dendritic spine

Inferred from electronic annotation. Source: UniProtKB-SubCell

filopodium

Inferred from sequence or structural similarity. Source: UniProtKB

integral component of membrane

Inferred from electronic annotation. Source: UniProtKB-KW

neuron projection

Inferred from sequence or structural similarity. Source: UniProtKB

neuronal cell body

Inferred from sequence or structural similarity. Source: UniProtKB

plasma membrane

Inferred from sequence or structural similarity. Source: UniProtKB

   Molecular_functioncalcium channel activity

Inferred from electronic annotation. Source: Ensembl

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 278278Neuronal membrane glycoprotein M6-a
PRO_0000159019

Regions

Topological domain1 – 2222Cytoplasmic Potential
Transmembrane23 – 4321Helical; Potential
Topological domain44 – 8441Extracellular Potential
Transmembrane85 – 10521Helical; Potential
Topological domain106 – 12722Cytoplasmic Potential
Transmembrane128 – 14821Helical; Potential
Topological domain149 – 21365Extracellular By similarity
Transmembrane214 – 23421Helical; Potential
Topological domain235 – 27844Cytoplasmic Potential

Amino acid modifications

Modified residue11N-acetylmethionine By similarity
Glycosylation1641N-linked (GlcNAc...) Potential
Glycosylation2081N-linked (GlcNAc...) Potential
Disulfide bond174 ↔ 192 By similarity

Sequences

Sequence LengthMass (Da)Tools
P35802 [UniParc].

Last modified June 1, 1994. Version 1.
Checksum: CEE0688873E786CE

FASTA27831,149
        10         20         30         40         50         60 
MEENMEEGQT QKGCFECCIK CLGGIPYASL IATILLYAGV ALFCGCGHEA LSGTVNILQT 

        70         80         90        100        110        120 
YFELARTAGD TLDVFTMIDI FKYVIYGIAA AFFVYGILLM VEGFFTTGAI KDLYGDFKIT 

       130        140        150        160        170        180 
TCGRCVSAWF IMLTYLFMLA WLGVTAFTSL PVYMYFNVWT ICRNTTLVEG ANLCLDLRQF 

       190        200        210        220        230        240 
GIVTIGEEKK ICTASENFLR MCESTELNMT FHLFIVALAG AGAAVIAMVH YLMVLSANWA 

       250        260        270 
YVKDACRMQK YEDIKSKEEQ ELHDIHSTRS KERLNAYT 

« Hide

References

« Hide 'large scale' references
[1]"Molecular cloning of M6: identification of a PLP/DM20 gene family."
Yan Y., Lagenaur C., Narayanan V.
Neuron 11:423-431(1993) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA], PROTEIN SEQUENCE OF 67-78.
Tissue: Brain.
[2]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Strain: C57BL/6.
Tissue: Brain, Eye and Retina.
[3]Lubec G., Kang S.U.
Submitted (APR-2007) to UniProtKB
Cited for: PROTEIN SEQUENCE OF 13-20; 67-82; 112-118; 179-200 AND 256-269, IDENTIFICATION BY MASS SPECTROMETRY.
Strain: C57BL/6.
Tissue: Brain.
[4]"M6a is expressed in the murine neural retina and regulates neurite extension."
Zhao J., Iida A., Ouchi Y., Satoh S., Watanabe S.
Mol. Vis. 14:1623-1630(2008) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, DEVELOPMENTAL STAGE, TISSUE SPECIFICITY.
[5]"Inhibition of mouse GPM6A expression leads to decreased differentiation of neurons derived from mouse embryonic stem cells."
Michibata H., Okuno T., Konishi N., Wakimoto K., Kyono K., Aoki K., Kondo Y., Takata K., Kitamura Y., Taniguchi T.
Stem Cells Dev. 17:641-651(2008) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION.
[6]"Induction of axon growth arrest without growth cone collapse through the N-terminal region of four-transmembrane glycoprotein M6a."
Sato Y., Mita S., Fukushima N., Fujisawa H., Saga Y., Hirata T.
Dev. Neurobiol. 71:733-746(2011) [PubMed] [Europe PMC] [Abstract]
Cited for: SUBCELLULAR LOCATION.
[7]"Actin-independent behavior and membrane deformation exhibited by the four-transmembrane protein M6a."
Sato Y., Watanabe N., Fukushima N., Mita S., Hirata T.
PLoS ONE 6:E26702-E26702(2011) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
S65735 mRNA. Translation: AAB28350.1.
BC024759 mRNA. Translation: AAH24759.1.
BC029683 mRNA. Translation: AAH29683.1.
BC029711 mRNA. Translation: AAH29711.1.
BC033357 mRNA. Translation: AAH33357.1.
BC033394 mRNA. Translation: AAH33394.1.
BC043130 mRNA. Translation: AAH43130.1.
CCDSCCDS22310.1.
RefSeqNP_001240683.1. NM_001253754.1.
NP_001240685.1. NM_001253756.1.
NP_705809.1. NM_153581.6.
UniGeneMm.241700.

3D structure databases

ProteinModelPortalP35802.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

IntActP35802. 5 interactions.
MINTMINT-4096535.
STRING10090.ENSMUSP00000033915.

Protein family/group databases

TCDB9.B.38.1.1. the myelin proteolipid protein (mplp) family.

PTM databases

PhosphoSiteP35802.

Proteomic databases

MaxQBP35802.
PaxDbP35802.
PRIDEP35802.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSMUST00000033915; ENSMUSP00000033915; ENSMUSG00000031517.
GeneID234267.
KEGGmmu:234267.
UCSCuc009lsl.2. mouse.

Organism-specific databases

CTD2823.
MGIMGI:107671. Gpm6a.

Phylogenomic databases

eggNOGNOG322245.
HOGENOMHOG000231338.
HOVERGENHBG000096.
InParanoidP35802.
OMAEEKKVCT.
OrthoDBEOG7X3QRG.
PhylomeDBP35802.
TreeFamTF315162.

Gene expression databases

ArrayExpressP35802.
BgeeP35802.
CleanExMM_GPM6A.
GenevestigatorP35802.

Family and domain databases

InterProIPR001614. Myelin_PLP.
IPR018237. Myelin_PLP_CS.
[Graphical view]
PANTHERPTHR11683. PTHR11683. 1 hit.
PfamPF01275. Myelin_PLP. 1 hit.
[Graphical view]
PRINTSPR00214. MYELINPLP.
SMARTSM00002. PLP. 1 hit.
[Graphical view]
PROSITEPS00575. MYELIN_PLP_1. 1 hit.
PS01004. MYELIN_PLP_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

ChiTaRSGPM6A. mouse.
NextBio382077.
PROP35802.
SOURCESearch...

Entry information

Entry nameGPM6A_MOUSE
AccessionPrimary (citable) accession number: P35802
Entry history
Integrated into UniProtKB/Swiss-Prot: June 1, 1994
Last sequence update: June 1, 1994
Last modified: July 9, 2014
This is version 111 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot