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P35802

- GPM6A_MOUSE

UniProt

P35802 - GPM6A_MOUSE

Protein

Neuronal membrane glycoprotein M6-a

Gene

Gpm6a

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 112 (01 Oct 2014)
      Sequence version 1 (01 Jun 1994)
      Previous versions | rss
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    Functioni

    Involved in neuronal differentiation, including differentiation and migration of neuronal stem cells. Plays a role in neuronal plasticity and is involved in neurite and filopodia outgrowth, filopodia motility and probably synapse formation. Gpm6a-induced filopodia formation involves mitogen-activated protein kinase (MAPK) and Src signaling pathways. Conflictingly, PubMed:22162747 reports that induced cellular protrusions are simple membrane-wrapped tubules without actin or tubulin-based cytoskeletons and with Gpm6a gliding along membrane edges indicative for a function in actin-independent membrane deformation. May be involved in neuronal NGF-dependent Ca2+ influx. May be involved in regulation of endocytosis and intracellular trafficking of G-protein-coupled receptors (GPCRs); enhances internalization and recycling of mu-type opioid receptor.3 Publications

    GO - Molecular functioni

    1. calcium channel activity Source: Ensembl

    GO - Biological processi

    1. neural retina development Source: UniProtKB
    2. neuron migration Source: UniProtKB
    3. neuron projection morphogenesis Source: UniProtKB
    4. positive regulation of filopodium assembly Source: UniProtKB
    5. stem cell differentiation Source: UniProtKB
    6. synapse assembly Source: UniProtKB

    Keywords - Biological processi

    Neurogenesis

    Protein family/group databases

    TCDBi9.B.38.1.1. the myelin proteolipid protein (mplp) family.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Neuronal membrane glycoprotein M6-a
    Short name:
    M6a
    Gene namesi
    Name:Gpm6a
    Synonyms:M6a
    OrganismiMus musculus (Mouse)
    Taxonomic identifieri10090 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
    ProteomesiUP000000589: Chromosome 8

    Organism-specific databases

    MGIiMGI:107671. Gpm6a.

    Subcellular locationi

    Cell membrane 1 Publication; Multi-pass membrane protein 1 Publication. Cell projectionaxon 1 Publication. Cell projectiondendritic spine By similarity. Cell projectionfilopodium By similarity
    Note: Localizes to cholesterol-rich lipid rafts of the plasma membrane of hippocampal neurons. Localized to plasma membrane of cell bodies and neurites of hippocampal neurons. Localized in membrane protrusions (filopodia and spines) of primary hippocampal neurons By similarity. Localized to the growth cone edge membrane of elongating axons.By similarity

    GO - Cellular componenti

    1. axonal growth cone Source: UniProtKB
    2. dendritic spine Source: UniProtKB-SubCell
    3. filopodium Source: UniProtKB
    4. integral component of membrane Source: UniProtKB-KW
    5. neuronal cell body Source: UniProtKB
    6. neuron projection Source: UniProtKB
    7. plasma membrane Source: UniProtKB

    Keywords - Cellular componenti

    Cell membrane, Cell projection, Membrane

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 278278Neuronal membrane glycoprotein M6-aPRO_0000159019Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei1 – 11N-acetylmethionineBy similarity
    Glycosylationi164 – 1641N-linked (GlcNAc...)Sequence Analysis
    Disulfide bondi174 ↔ 192By similarity
    Glycosylationi208 – 2081N-linked (GlcNAc...)Sequence Analysis

    Keywords - PTMi

    Acetylation, Disulfide bond, Glycoprotein

    Proteomic databases

    MaxQBiP35802.
    PaxDbiP35802.
    PRIDEiP35802.

    PTM databases

    PhosphoSiteiP35802.

    Expressioni

    Tissue specificityi

    Widely expressed in the CNS. Found especially in the granule cell layer of the cerebellum but not in the molecular layer or white matter. Expressed in the immature embryonic retina including the nerve fiber layer (NFL), inner plexiform layer (IPL), and outer plexiform layer (OPL). Weakly expressed in processes of Mueller glia cells.1 Publication

    Developmental stagei

    First detected in presumptive postmitotic neurons in the developing neural tube at embryonic day 9. Expressed in E14 retinas, and expression continued after birth with a slight decrease between P12 and P15. In the E14 retina is mainly and strongly expressed in the NFL. In P1 and P5 retinas strong expression is confined to the IPL and also in NFL. At P10 and in the adult retina strong expression is detected in the IPL, and weak expression in NFL, OPL, and inner nuclear layer. Is expressed on postmitotic mature neurons.1 Publication

    Gene expression databases

    ArrayExpressiP35802.
    BgeeiP35802.
    CleanExiMM_GPM6A.
    GenevestigatoriP35802.

    Interactioni

    Protein-protein interaction databases

    IntActiP35802. 5 interactions.
    MINTiMINT-4096535.
    STRINGi10090.ENSMUSP00000033915.

    Structurei

    3D structure databases

    ProteinModelPortaliP35802.
    ModBaseiSearch...
    MobiDBiSearch...

    Topological domain

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Topological domaini1 – 2222CytoplasmicSequence AnalysisAdd
    BLAST
    Topological domaini44 – 8441ExtracellularSequence AnalysisAdd
    BLAST
    Topological domaini106 – 12722CytoplasmicSequence AnalysisAdd
    BLAST
    Topological domaini149 – 21365ExtracellularBy similarityAdd
    BLAST
    Topological domaini235 – 27844CytoplasmicSequence AnalysisAdd
    BLAST

    Transmembrane

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transmembranei23 – 4321HelicalSequence AnalysisAdd
    BLAST
    Transmembranei85 – 10521HelicalSequence AnalysisAdd
    BLAST
    Transmembranei128 – 14821HelicalSequence AnalysisAdd
    BLAST
    Transmembranei214 – 23421HelicalSequence AnalysisAdd
    BLAST

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the myelin proteolipid protein family.Curated

    Keywords - Domaini

    Transmembrane, Transmembrane helix

    Phylogenomic databases

    eggNOGiNOG322245.
    HOGENOMiHOG000231338.
    HOVERGENiHBG000096.
    InParanoidiP35802.
    OMAiEEKKVCT.
    OrthoDBiEOG7X3QRG.
    PhylomeDBiP35802.
    TreeFamiTF315162.

    Family and domain databases

    InterProiIPR001614. Myelin_PLP.
    IPR018237. Myelin_PLP_CS.
    [Graphical view]
    PANTHERiPTHR11683. PTHR11683. 1 hit.
    PfamiPF01275. Myelin_PLP. 1 hit.
    [Graphical view]
    PRINTSiPR00214. MYELINPLP.
    SMARTiSM00002. PLP. 1 hit.
    [Graphical view]
    PROSITEiPS00575. MYELIN_PLP_1. 1 hit.
    PS01004. MYELIN_PLP_2. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P35802-1 [UniParc]FASTAAdd to Basket

    « Hide

    MEENMEEGQT QKGCFECCIK CLGGIPYASL IATILLYAGV ALFCGCGHEA    50
    LSGTVNILQT YFELARTAGD TLDVFTMIDI FKYVIYGIAA AFFVYGILLM 100
    VEGFFTTGAI KDLYGDFKIT TCGRCVSAWF IMLTYLFMLA WLGVTAFTSL 150
    PVYMYFNVWT ICRNTTLVEG ANLCLDLRQF GIVTIGEEKK ICTASENFLR 200
    MCESTELNMT FHLFIVALAG AGAAVIAMVH YLMVLSANWA YVKDACRMQK 250
    YEDIKSKEEQ ELHDIHSTRS KERLNAYT 278
    Length:278
    Mass (Da):31,149
    Last modified:June 1, 1994 - v1
    Checksum:iCEE0688873E786CE
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    S65735 mRNA. Translation: AAB28350.1.
    BC024759 mRNA. Translation: AAH24759.1.
    BC029683 mRNA. Translation: AAH29683.1.
    BC029711 mRNA. Translation: AAH29711.1.
    BC033357 mRNA. Translation: AAH33357.1.
    BC033394 mRNA. Translation: AAH33394.1.
    BC043130 mRNA. Translation: AAH43130.1.
    CCDSiCCDS22310.1.
    RefSeqiNP_001240683.1. NM_001253754.1.
    NP_001240685.1. NM_001253756.1.
    NP_705809.1. NM_153581.6.
    UniGeneiMm.241700.

    Genome annotation databases

    EnsembliENSMUST00000033915; ENSMUSP00000033915; ENSMUSG00000031517.
    GeneIDi234267.
    KEGGimmu:234267.
    UCSCiuc009lsl.2. mouse.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    S65735 mRNA. Translation: AAB28350.1 .
    BC024759 mRNA. Translation: AAH24759.1 .
    BC029683 mRNA. Translation: AAH29683.1 .
    BC029711 mRNA. Translation: AAH29711.1 .
    BC033357 mRNA. Translation: AAH33357.1 .
    BC033394 mRNA. Translation: AAH33394.1 .
    BC043130 mRNA. Translation: AAH43130.1 .
    CCDSi CCDS22310.1.
    RefSeqi NP_001240683.1. NM_001253754.1.
    NP_001240685.1. NM_001253756.1.
    NP_705809.1. NM_153581.6.
    UniGenei Mm.241700.

    3D structure databases

    ProteinModelPortali P35802.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    IntActi P35802. 5 interactions.
    MINTi MINT-4096535.
    STRINGi 10090.ENSMUSP00000033915.

    Protein family/group databases

    TCDBi 9.B.38.1.1. the myelin proteolipid protein (mplp) family.

    PTM databases

    PhosphoSitei P35802.

    Proteomic databases

    MaxQBi P35802.
    PaxDbi P35802.
    PRIDEi P35802.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSMUST00000033915 ; ENSMUSP00000033915 ; ENSMUSG00000031517 .
    GeneIDi 234267.
    KEGGi mmu:234267.
    UCSCi uc009lsl.2. mouse.

    Organism-specific databases

    CTDi 2823.
    MGIi MGI:107671. Gpm6a.

    Phylogenomic databases

    eggNOGi NOG322245.
    HOGENOMi HOG000231338.
    HOVERGENi HBG000096.
    InParanoidi P35802.
    OMAi EEKKVCT.
    OrthoDBi EOG7X3QRG.
    PhylomeDBi P35802.
    TreeFami TF315162.

    Miscellaneous databases

    ChiTaRSi GPM6A. mouse.
    NextBioi 382077.
    PROi P35802.
    SOURCEi Search...

    Gene expression databases

    ArrayExpressi P35802.
    Bgeei P35802.
    CleanExi MM_GPM6A.
    Genevestigatori P35802.

    Family and domain databases

    InterProi IPR001614. Myelin_PLP.
    IPR018237. Myelin_PLP_CS.
    [Graphical view ]
    PANTHERi PTHR11683. PTHR11683. 1 hit.
    Pfami PF01275. Myelin_PLP. 1 hit.
    [Graphical view ]
    PRINTSi PR00214. MYELINPLP.
    SMARTi SM00002. PLP. 1 hit.
    [Graphical view ]
    PROSITEi PS00575. MYELIN_PLP_1. 1 hit.
    PS01004. MYELIN_PLP_2. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Molecular cloning of M6: identification of a PLP/DM20 gene family."
      Yan Y., Lagenaur C., Narayanan V.
      Neuron 11:423-431(1993) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA], PROTEIN SEQUENCE OF 67-78.
      Tissue: Brain.
    2. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Strain: C57BL/6.
      Tissue: Brain, Eye and Retina.
    3. Lubec G., Kang S.U.
      Submitted (APR-2007) to UniProtKB
      Cited for: PROTEIN SEQUENCE OF 13-20; 67-82; 112-118; 179-200 AND 256-269, IDENTIFICATION BY MASS SPECTROMETRY.
      Strain: C57BL/6.
      Tissue: Brain.
    4. "M6a is expressed in the murine neural retina and regulates neurite extension."
      Zhao J., Iida A., Ouchi Y., Satoh S., Watanabe S.
      Mol. Vis. 14:1623-1630(2008) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, DEVELOPMENTAL STAGE, TISSUE SPECIFICITY.
    5. "Inhibition of mouse GPM6A expression leads to decreased differentiation of neurons derived from mouse embryonic stem cells."
      Michibata H., Okuno T., Konishi N., Wakimoto K., Kyono K., Aoki K., Kondo Y., Takata K., Kitamura Y., Taniguchi T.
      Stem Cells Dev. 17:641-651(2008) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION.
    6. "Induction of axon growth arrest without growth cone collapse through the N-terminal region of four-transmembrane glycoprotein M6a."
      Sato Y., Mita S., Fukushima N., Fujisawa H., Saga Y., Hirata T.
      Dev. Neurobiol. 71:733-746(2011) [PubMed] [Europe PMC] [Abstract]
      Cited for: SUBCELLULAR LOCATION.
    7. "Actin-independent behavior and membrane deformation exhibited by the four-transmembrane protein M6a."
      Sato Y., Watanabe N., Fukushima N., Mita S., Hirata T.
      PLoS ONE 6:E26702-E26702(2011) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION.

    Entry informationi

    Entry nameiGPM6A_MOUSE
    AccessioniPrimary (citable) accession number: P35802
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: June 1, 1994
    Last sequence update: June 1, 1994
    Last modified: October 1, 2014
    This is version 112 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Direct protein sequencing, Reference proteome

    Documents

    1. MGD cross-references
      Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3