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Protein

Neuronal membrane glycoprotein M6-a

Gene

Gpm6a

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Involved in neuronal differentiation, including differentiation and migration of neuronal stem cells. Plays a role in neuronal plasticity and is involved in neurite and filopodia outgrowth, filopodia motility and probably synapse formation. Gpm6a-induced filopodia formation involves mitogen-activated protein kinase (MAPK) and Src signaling pathways. Conflictingly, PubMed:22162747 reports that induced cellular protrusions are simple membrane-wrapped tubules without actin or tubulin-based cytoskeletons and with Gpm6a gliding along membrane edges indicative for a function in actin-independent membrane deformation. May be involved in neuronal NGF-dependent Ca2+ influx. May be involved in regulation of endocytosis and intracellular trafficking of G-protein-coupled receptors (GPCRs); enhances internalization and recycling of mu-type opioid receptor.3 Publications

GO - Molecular functioni

GO - Biological processi

  • neural retina development Source: UniProtKB
  • neuron migration Source: UniProtKB
  • neuron projection morphogenesis Source: UniProtKB
  • positive regulation of filopodium assembly Source: UniProtKB
  • stem cell differentiation Source: UniProtKB
  • synapse assembly Source: UniProtKB
Complete GO annotation...

Keywords - Biological processi

Neurogenesis

Protein family/group databases

TCDBi9.B.38.1.1. the myelin proteolipid protein (mplp) family.

Names & Taxonomyi

Protein namesi
Recommended name:
Neuronal membrane glycoprotein M6-a
Short name:
M6a
Gene namesi
Name:Gpm6a
Synonyms:M6a
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589 Componenti: Chromosome 8

Organism-specific databases

MGIiMGI:107671. Gpm6a.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 2222CytoplasmicSequence AnalysisAdd
BLAST
Transmembranei23 – 4321HelicalSequence AnalysisAdd
BLAST
Topological domaini44 – 8441ExtracellularSequence AnalysisAdd
BLAST
Transmembranei85 – 10521HelicalSequence AnalysisAdd
BLAST
Topological domaini106 – 12722CytoplasmicSequence AnalysisAdd
BLAST
Transmembranei128 – 14821HelicalSequence AnalysisAdd
BLAST
Topological domaini149 – 21365ExtracellularBy similarityAdd
BLAST
Transmembranei214 – 23421HelicalSequence AnalysisAdd
BLAST
Topological domaini235 – 27844CytoplasmicSequence AnalysisAdd
BLAST

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Cell projection, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 278278Neuronal membrane glycoprotein M6-aPRO_0000159019Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei1 – 11N-acetylmethionineBy similarity
Glycosylationi164 – 1641N-linked (GlcNAc...)Sequence Analysis
Disulfide bondi174 ↔ 192By similarity
Glycosylationi208 – 2081N-linked (GlcNAc...)Sequence Analysis

Keywords - PTMi

Acetylation, Disulfide bond, Glycoprotein

Proteomic databases

MaxQBiP35802.
PaxDbiP35802.
PRIDEiP35802.

PTM databases

PhosphoSiteiP35802.

Expressioni

Tissue specificityi

Widely expressed in the CNS. Found especially in the granule cell layer of the cerebellum but not in the molecular layer or white matter. Expressed in the immature embryonic retina including the nerve fiber layer (NFL), inner plexiform layer (IPL), and outer plexiform layer (OPL). Weakly expressed in processes of Mueller glia cells.1 Publication

Developmental stagei

First detected in presumptive postmitotic neurons in the developing neural tube at embryonic day 9. Expressed in E14 retinas, and expression continued after birth with a slight decrease between P12 and P15. In the E14 retina is mainly and strongly expressed in the NFL. In P1 and P5 retinas strong expression is confined to the IPL and also in NFL. At P10 and in the adult retina strong expression is detected in the IPL, and weak expression in NFL, OPL, and inner nuclear layer. Is expressed on postmitotic mature neurons.1 Publication

Gene expression databases

BgeeiP35802.
CleanExiMM_GPM6A.
ExpressionAtlasiP35802. baseline and differential.
GenevisibleiP35802. MM.

Interactioni

Protein-protein interaction databases

BioGridi231507. 1 interaction.
IntActiP35802. 5 interactions.
MINTiMINT-4096535.
STRINGi10090.ENSMUSP00000033915.

Structurei

3D structure databases

ProteinModelPortaliP35802.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the myelin proteolipid protein family.Curated

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiNOG322245.
HOGENOMiHOG000231338.
HOVERGENiHBG000096.
InParanoidiP35802.
OMAiWIEAVQM.
OrthoDBiEOG7X3QRG.
PhylomeDBiP35802.
TreeFamiTF315162.

Family and domain databases

InterProiIPR001614. Myelin_PLP.
IPR018237. Myelin_PLP_CS.
[Graphical view]
PANTHERiPTHR11683. PTHR11683. 1 hit.
PfamiPF01275. Myelin_PLP. 1 hit.
[Graphical view]
PRINTSiPR00214. MYELINPLP.
SMARTiSM00002. PLP. 1 hit.
[Graphical view]
PROSITEiPS00575. MYELIN_PLP_1. 1 hit.
PS01004. MYELIN_PLP_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P35802-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MEENMEEGQT QKGCFECCIK CLGGIPYASL IATILLYAGV ALFCGCGHEA
60 70 80 90 100
LSGTVNILQT YFELARTAGD TLDVFTMIDI FKYVIYGIAA AFFVYGILLM
110 120 130 140 150
VEGFFTTGAI KDLYGDFKIT TCGRCVSAWF IMLTYLFMLA WLGVTAFTSL
160 170 180 190 200
PVYMYFNVWT ICRNTTLVEG ANLCLDLRQF GIVTIGEEKK ICTASENFLR
210 220 230 240 250
MCESTELNMT FHLFIVALAG AGAAVIAMVH YLMVLSANWA YVKDACRMQK
260 270
YEDIKSKEEQ ELHDIHSTRS KERLNAYT
Length:278
Mass (Da):31,149
Last modified:June 1, 1994 - v1
Checksum:iCEE0688873E786CE
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
S65735 mRNA. Translation: AAB28350.1.
BC024759 mRNA. Translation: AAH24759.1.
BC029683 mRNA. Translation: AAH29683.1.
BC029711 mRNA. Translation: AAH29711.1.
BC033357 mRNA. Translation: AAH33357.1.
BC033394 mRNA. Translation: AAH33394.1.
BC043130 mRNA. Translation: AAH43130.1.
CCDSiCCDS22310.1.
RefSeqiNP_001240683.1. NM_001253754.1.
NP_001240685.1. NM_001253756.1.
NP_705809.1. NM_153581.6.
UniGeneiMm.241700.

Genome annotation databases

EnsembliENSMUST00000033915; ENSMUSP00000033915; ENSMUSG00000031517.
GeneIDi234267.
KEGGimmu:234267.
UCSCiuc009lsl.2. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
S65735 mRNA. Translation: AAB28350.1.
BC024759 mRNA. Translation: AAH24759.1.
BC029683 mRNA. Translation: AAH29683.1.
BC029711 mRNA. Translation: AAH29711.1.
BC033357 mRNA. Translation: AAH33357.1.
BC033394 mRNA. Translation: AAH33394.1.
BC043130 mRNA. Translation: AAH43130.1.
CCDSiCCDS22310.1.
RefSeqiNP_001240683.1. NM_001253754.1.
NP_001240685.1. NM_001253756.1.
NP_705809.1. NM_153581.6.
UniGeneiMm.241700.

3D structure databases

ProteinModelPortaliP35802.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi231507. 1 interaction.
IntActiP35802. 5 interactions.
MINTiMINT-4096535.
STRINGi10090.ENSMUSP00000033915.

Protein family/group databases

TCDBi9.B.38.1.1. the myelin proteolipid protein (mplp) family.

PTM databases

PhosphoSiteiP35802.

Proteomic databases

MaxQBiP35802.
PaxDbiP35802.
PRIDEiP35802.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000033915; ENSMUSP00000033915; ENSMUSG00000031517.
GeneIDi234267.
KEGGimmu:234267.
UCSCiuc009lsl.2. mouse.

Organism-specific databases

CTDi2823.
MGIiMGI:107671. Gpm6a.

Phylogenomic databases

eggNOGiNOG322245.
HOGENOMiHOG000231338.
HOVERGENiHBG000096.
InParanoidiP35802.
OMAiWIEAVQM.
OrthoDBiEOG7X3QRG.
PhylomeDBiP35802.
TreeFamiTF315162.

Miscellaneous databases

ChiTaRSiGpm6a. mouse.
NextBioi382077.
PROiP35802.
SOURCEiSearch...

Gene expression databases

BgeeiP35802.
CleanExiMM_GPM6A.
ExpressionAtlasiP35802. baseline and differential.
GenevisibleiP35802. MM.

Family and domain databases

InterProiIPR001614. Myelin_PLP.
IPR018237. Myelin_PLP_CS.
[Graphical view]
PANTHERiPTHR11683. PTHR11683. 1 hit.
PfamiPF01275. Myelin_PLP. 1 hit.
[Graphical view]
PRINTSiPR00214. MYELINPLP.
SMARTiSM00002. PLP. 1 hit.
[Graphical view]
PROSITEiPS00575. MYELIN_PLP_1. 1 hit.
PS01004. MYELIN_PLP_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Molecular cloning of M6: identification of a PLP/DM20 gene family."
    Yan Y., Lagenaur C., Narayanan V.
    Neuron 11:423-431(1993) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], PROTEIN SEQUENCE OF 67-78.
    Tissue: Brain.
  2. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: C57BL/6.
    Tissue: Brain, Eye and Retina.
  3. Lubec G., Kang S.U.
    Submitted (APR-2007) to UniProtKB
    Cited for: PROTEIN SEQUENCE OF 13-20; 67-82; 112-118; 179-200 AND 256-269, IDENTIFICATION BY MASS SPECTROMETRY.
    Strain: C57BL/6.
    Tissue: Brain.
  4. "M6a is expressed in the murine neural retina and regulates neurite extension."
    Zhao J., Iida A., Ouchi Y., Satoh S., Watanabe S.
    Mol. Vis. 14:1623-1630(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, DEVELOPMENTAL STAGE, TISSUE SPECIFICITY.
  5. "Inhibition of mouse GPM6A expression leads to decreased differentiation of neurons derived from mouse embryonic stem cells."
    Michibata H., Okuno T., Konishi N., Wakimoto K., Kyono K., Aoki K., Kondo Y., Takata K., Kitamura Y., Taniguchi T.
    Stem Cells Dev. 17:641-651(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  6. "Induction of axon growth arrest without growth cone collapse through the N-terminal region of four-transmembrane glycoprotein M6a."
    Sato Y., Mita S., Fukushima N., Fujisawa H., Saga Y., Hirata T.
    Dev. Neurobiol. 71:733-746(2011) [PubMed] [Europe PMC] [Abstract]
    Cited for: SUBCELLULAR LOCATION.
  7. "Actin-independent behavior and membrane deformation exhibited by the four-transmembrane protein M6a."
    Sato Y., Watanabe N., Fukushima N., Mita S., Hirata T.
    PLoS ONE 6:E26702-E26702(2011) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.

Entry informationi

Entry nameiGPM6A_MOUSE
AccessioniPrimary (citable) accession number: P35802
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 1, 1994
Last sequence update: June 1, 1994
Last modified: June 24, 2015
This is version 120 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.