Reviewed, UniProtKB/Swiss-Prot P35790 (CHKA_HUMAN)

Names and origin

Protein names

Recommended name:
    Choline kinase alpha
      Short name=CK
    EC=2.7.1.32
Alternative name(s):
    CHETK-alpha

Gene names

Name:	CHKA
Synonyms:	CHK, CKI

Organism

Homo sapiens (Human)

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo

Protein attributes

Sequence length	457 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	May have a regulatory role in phosphatidylcholine synthesis.
Catalytic activity	ATP + choline = ADP + O-phosphocholine.
Pathway	Phospholipid metabolism; phosphatidylcholine biosynthesis; phosphocholine from choline: step 1/1.
Subunit structure	Homotetramer By similarity.
Subcellular location	Cytoplasm.
Sequence similarities	Belongs to the choline/ethanolamine kinase family.

Ontologies

Keywords
Cellular component	Cytoplasm
Coding sequence diversity	Alternative splicing Polymorphism
Ligand	ATP-binding Nucleotide-binding
Molecular function	Kinase Transferase
Technical term	3D-structure
Gene Ontology (GO)
Biological process	lipid transport Traceable author statement. Source: ProtInc
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW choline kinase activity Traceable author statement. Source: ProtInc signal transducer activity Traceable author statement. Source: ProtInc
Complete GO annotation...

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 (identifier: P35790-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 (identifier: P35790-2)

The sequence of this isoform differs from the canonical sequence as follows:
     155-172: Missing.

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 457

457

Choline kinase alpha

PRO_0000206219

Regions

Compositional bias

50 – 85

36

Pro-rich

Sites

Active site

304

1

By similarity

Natural variations

Alternative sequence

155 – 172

18

Missing in isoform 2.

VSP_009683

Natural variant

220

1

S → G: dbSNP rs17853641. Ref.3

VAR_054863

Natural variant

422

1

L → Q: dbSNP rs17853642. Ref.3

VAR_054864

Experimental info

Sequence conflict

49 – 54

6

GGQQPP → APTAA in BAA01547. Ref.1

Sequence conflict

87

1

T → A in BAA01547. Ref.1

Sequence conflict

154

1

M → V in BAA01547. Ref.1

Secondary structure

1

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457

Helix Strand Turn

Details...

Helix

89 – 98

10

Helix

101 – 103

3

Helix

108 – 110

3

Beta strand

112 – 117

6

Beta strand

123 – 128

6

Beta strand

136 – 138

3

Beta strand

141 – 147

7

Helix

178 – 189

12

Beta strand

196 – 200

5

Beta strand

203 – 207

5

Beta strand

211 – 213

3

Helix

216 – 220

5

Helix

222 – 236

15

Helix

248 – 260

13

Helix

268 – 278

11

Helix

282 – 294

13

Beta strand

300 – 303

4

Helix

309 – 311

3

Beta strand

312 – 315

4

Beta strand

326 – 328

3

Beta strand

335 – 338

4

Helix

339 – 349

11

Helix

366 – 368

3

Helix

372 – 386

15

Helix

395 – 430

36

Beta strand

433 – 435

3

Helix

437 – 455

19

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 [UniParc]. Last modified April 14, 2009. Version 3. Checksum: 65F177ABE1AA3A12	FASTA	457	52,249
	10 20 30 40 50 60 MKTKFCTGGE AEPSPLGLLL SCGSGSAAPA PGVGQQRDAA SDLESKQLGG QQPPLALPPP 70 80 90 100 110 120 PPLPLPLPLP QPPPPQPPAD EQPEPRTRRR AYLWCKEFLP GAWRGLREDE FHISVIRGGL 130 140 150 160 170 180 SNMLFQCSLP DTTATLGDEP RKVLLRLYGA ILQMRSCNKE GSEQAQKENE FQGAEAMVLE 190 200 210 220 230 240 SVMFAILAER SLGPKLYGIF PQGRLEQFIP SRRLDTEELS LPDISAEIAE KMATFHGMKM 250 260 270 280 290 300 PFNKEPKWLF GTMEKYLKEV LRIKFTEESR IKKLHKLLSY NLPLELENLR SLLESTPSPV 310 320 330 340 350 360 VFCHNDCQEG NILLLEGREN SEKQKLMLID FEYSSYNYRG FDIGNHFCEW MYDYSYEKYP 370 380 390 400 410 420 FFRANIRKYP TKKQQLHFIS SYLPAFQNDF ENLSTEEKSI IKEEMLLEVN RFALASHFLW 430 440 450 GLWSIVQAKI SSIEFGYMDY AQARFDAYFH QKRKLGV
	Isoform 2. Checksum: 73507D716F15F9E4	FASTA	439	50,155
	10 20 30 40 50 60 MKTKFCTGGE AEPSPLGLLL SCGSGSAAPA PGVGQQRDAA SDLESKQLGG QQPPLALPPP 70 80 90 100 110 120 PPLPLPLPLP QPPPPQPPAD EQPEPRTRRR AYLWCKEFLP GAWRGLREDE FHISVIRGGL 130 140 150 160 170 180 SNMLFQCSLP DTTATLGDEP RKVLLRLYGA ILQMGAEAMV LESVMFAILA ERSLGPKLYG 190 200 210 220 230 240 IFPQGRLEQF IPSRRLDTEE LSLPDISAEI AEKMATFHGM KMPFNKEPKW LFGTMEKYLK 250 260 270 280 290 300 EVLRIKFTEE SRIKKLHKLL SYNLPLELEN LRSLLESTPS PVVFCHNDCQ EGNILLLEGR 310 320 330 340 350 360 ENSEKQKLML IDFEYSSYNY RGFDIGNHFC EWMYDYSYEK YPFFRANIRK YPTKKQQLHF 370 380 390 400 410 420 ISSYLPAFQN DFENLSTEEK SIIKEEMLLE VNRFALASHF LWGLWSIVQA KISSIEFGYM 430 DYAQARFDAY FHQKRKLGV

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Cloning of a human choline kinase cDNA by complementation of the yeast cki mutation." Hosaka K., Tanaka S., Nikawa J., Yamashita S. FEBS Lett. 304:229-232(1992) [PubMed: 1618328] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
[2]	"Human chromosome 11 DNA sequence and analysis including novel gene identification." Taylor T.D., Noguchi H., Totoki Y., Toyoda A., Kuroki Y., Dewar K., Lloyd C., Itoh T., Takeda T., Kim D.-W., She X., Barlow K.F., Bloom T., Bruford E., Chang J.L., Cuomo C.A., Eichler E., FitzGerald M.G. , Jaffe D.B., LaButti K., Nicol R., Park H.-S., Seaman C., Sougnez C., Yang X., Zimmer A.R., Zody M.C., Birren B.W., Nusbaum C., Fujiyama A., Hattori M., Rogers J., Lander E.S., Sakaki Y. Nature 440:497-500(2006) [PubMed: 16554811] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[3]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2), VARIANTS GLY-220 AND GLN-422. Tissue: Testis.
+	Additional computationally mapped references.

Web resources

Atlas of Genetics and Cytogenetics in Oncology and Haematology

Cross-references

Sequence databases

EMBLGenBankDDBJ

D10704 mRNA. Translation: BAA01547.1.
AP002807 Genomic DNA. No translation available.
AP002992 Genomic DNA. No translation available.
BC036471 mRNA. Translation: AAH36471.1.

IPI

IPI00409761.
IPI00409762.

PIR

S23104.

RefSeq

NP_001268.2.
NP_997634.1.

UniGene

Hs.495985
Hs.569019

3D structure databases

PDBPDBe

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
2CKO	X-ray	2.15	A/B	50-439	[»]
2CKP	X-ray	3.10	A/B	50-439	[»]
2CKQ	X-ray	2.40	A/B	50-439	[»]
2I7Q	X-ray	1.90	A	75-457	[»]
3F2R	X-ray	2.35	A/B	75-457	[»]
3G15	X-ray	1.70	A/B	75-457	[»]

ModBase

Search...

Genome annotation databases

Ensembl

ENSG00000110721. Homo sapiens. [Contig view]

GeneID

1119.

KEGG

hsa:1119.

Organism-specific databases

GeneCards

GC11M067576.

H-InvDB

HIX0017419.

HGNC

HGNC:1937. CHKA.

MIM

118491. gene.

PharmGKB

PA26468.

GenAtlas

Search...

Phylogenomic databases

HOGENOM

P35790.

HOVERGEN

P35790.

OMA

P35790. FLPGAWR.

Enzyme and pathway databases

BRENDA

2.7.1.32. 247.

Gene expression databases

ArrayExpress

P35790.

Bgee

P35790.

CleanEx

HS_CHKA.

GermOnline

ENSG00000110721. Homo sapiens.

Family and domain databases

InterPro

IPR002573. Choline/ethanolamine_kinase.
[Graphical view]

PANTHER

PTHR22603. Choline/ethanolamine_kinase. 1 hit.

Pfam

PF01633. Choline_kinase. 1 hit.
[Graphical view]

ProtoNet

Search...

Other Resources

DrugBank

DB00122. Choline.

NextBio

4642.

SOURCE

Search...

Entry information

Entry name

CHKA_HUMAN

Accession

Primary (citable) accession number: P35790
Secondary accession number(s): Q8NE29

Entry history

Integrated into UniProtKB/Swiss-Prot:	June 1, 1994
Last sequence update:	April 14, 2009
Last modified:	June 16, 2009
This is version 83 of the entry and version 3 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)