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Protein

Choline kinase alpha

Gene

CHKA

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Has a key role in phospholipid biosynthesis and may contribute to tumor cell growth. Catalyzes the first step in phosphatidylcholine biosynthesis. Contributes to phosphatidylethanolamine biosynthesis. Phosphorylates choline and ethanolamine. Has higher activity with choline.1 Publication

Catalytic activityi

ATP + choline = ADP + phosphocholine.1 Publication
ATP + ethanolamine = ADP + O-phosphoethanolamine.1 Publication

Kineticsi

  1. KM=0.2 mM for choline2 Publications
  2. KM=0.4 mM for ATP2 Publications
  3. KM=12 mM for ethanolamine2 Publications

    Pathwayi: phosphatidylcholine biosynthesis

    This protein is involved in step 1 of the subpathway that synthesizes phosphocholine from choline.
    Proteins known to be involved in this subpathway in this organism are:
    1. Choline kinase alpha (CHKA)
    This subpathway is part of the pathway phosphatidylcholine biosynthesis, which is itself part of Phospholipid metabolism.
    View all proteins of this organism that are known to be involved in the subpathway that synthesizes phosphocholine from choline, the pathway phosphatidylcholine biosynthesis and in Phospholipid metabolism.

    Pathwayi: phosphatidylethanolamine biosynthesis

    This protein is involved in step 1 of the subpathway that synthesizes phosphatidylethanolamine from ethanolamine.
    Proteins known to be involved in the 3 steps of the subpathway in this organism are:
    1. Choline/ethanolamine kinase (CHKB), Ethanolamine kinase 2 (ETNK2), Choline kinase alpha (CHKA), Ethanolamine kinase 1 (ETNK1)
    2. Ethanolamine-phosphate cytidylyltransferase (PCYT2)
    3. Ethanolaminephosphotransferase 1 (EPT1), Choline/ethanolaminephosphotransferase 1 (CEPT1)
    This subpathway is part of the pathway phosphatidylethanolamine biosynthesis, which is itself part of Phospholipid metabolism.
    View all proteins of this organism that are known to be involved in the subpathway that synthesizes phosphatidylethanolamine from ethanolamine, the pathway phosphatidylethanolamine biosynthesis and in Phospholipid metabolism.

    Sites

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Binding sitei146ATP1
    Binding sitei308ATPCurated1
    Binding sitei330ATP1

    Regions

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Nucleotide bindingi117 – 123ATP7
    Nucleotide bindingi207 – 213ATP7

    GO - Molecular functioni

    • ATP binding Source: UniProtKB-KW
    • choline binding Source: Ensembl
    • choline kinase activity Source: UniProtKB
    • cholinesterase activity Source: Ensembl
    • drug binding Source: UniProtKB
    • ethanolamine kinase activity Source: UniProtKB
    • signal transducer activity Source: ProtInc

    GO - Biological processi

    • choline metabolic process Source: Ensembl
    • ethanolamine metabolic process Source: Ensembl
    • lipid metabolic process Source: ProtInc
    • lipid transport Source: ProtInc
    • phosphatidylcholine biosynthetic process Source: UniProtKB
    • phosphatidylethanolamine biosynthetic process Source: UniProtKB
    • response to 3-methylcholanthrene Source: Ensembl
    • response to toxic substance Source: Ensembl
    Complete GO annotation...

    Keywords - Molecular functioni

    Kinase, Transferase

    Keywords - Biological processi

    Lipid biosynthesis, Lipid metabolism, Phospholipid biosynthesis, Phospholipid metabolism

    Keywords - Ligandi

    ATP-binding, Nucleotide-binding

    Enzyme and pathway databases

    BioCyciMetaCyc:HS03334-MONOMER.
    ZFISH:HS03334-MONOMER.
    BRENDAi2.7.1.32. 2681.
    ReactomeiR-HSA-1483191. Synthesis of PC.
    R-HSA-1483213. Synthesis of PE.
    SABIO-RKP35790.
    UniPathwayiUPA00558; UER00741.
    UPA00753; UER00737.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Choline kinase alpha (EC:2.7.1.32)
    Short name:
    CK
    Alternative name(s):
    CHETK-alpha
    Ethanolamine kinase (EC:2.7.1.82)
    Short name:
    EK
    Gene namesi
    Name:CHKA
    Synonyms:CHK, CKI
    OrganismiHomo sapiens (Human)
    Taxonomic identifieri9606 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
    Proteomesi
    • UP000005640 Componenti: Chromosome 11

    Organism-specific databases

    HGNCiHGNC:1937. CHKA.

    Subcellular locationi

    GO - Cellular componenti

    Complete GO annotation...

    Keywords - Cellular componenti

    Cytoplasm

    Pathology & Biotechi

    Organism-specific databases

    DisGeNETi1119.
    OpenTargetsiENSG00000110721.
    PharmGKBiPA26468.

    Chemistry databases

    ChEMBLiCHEMBL3117.
    DrugBankiDB00122. Choline.

    Polymorphism and mutation databases

    BioMutaiCHKA.
    DMDMi226694197.

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    ChainiPRO_00002062191 – 457Choline kinase alphaAdd BLAST457

    Proteomic databases

    EPDiP35790.
    MaxQBiP35790.
    PaxDbiP35790.
    PeptideAtlasiP35790.
    PRIDEiP35790.

    PTM databases

    iPTMnetiP35790.
    PhosphoSitePlusiP35790.

    Expressioni

    Gene expression databases

    BgeeiENSG00000110721.
    CleanExiHS_CHKA.
    ExpressionAtlasiP35790. baseline and differential.
    GenevisibleiP35790. HS.

    Organism-specific databases

    HPAiCAB072803.
    HPA024153.

    Interactioni

    Subunit structurei

    Heterodimer with CHKB (By similarity). Homodimer.By similarity2 Publications

    Protein-protein interaction databases

    BioGridi107543. 5 interactors.
    IntActiP35790. 1 interactor.
    STRINGi9606.ENSP00000265689.

    Chemistry databases

    BindingDBiP35790.

    Structurei

    Secondary structure

    1457
    Legend: HelixTurnBeta strandPDB Structure known for this area
    Show more details
    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Helixi85 – 98Combined sources14
    Helixi101 – 105Combined sources5
    Helixi108 – 110Combined sources3
    Beta strandi112 – 117Combined sources6
    Beta strandi119 – 128Combined sources10
    Beta strandi136 – 138Combined sources3
    Beta strandi141 – 147Combined sources7
    Helixi176 – 189Combined sources14
    Beta strandi196 – 200Combined sources5
    Beta strandi203 – 207Combined sources5
    Beta strandi211 – 213Combined sources3
    Helixi216 – 220Combined sources5
    Helixi222 – 236Combined sources15
    Helixi248 – 262Combined sources15
    Helixi268 – 278Combined sources11
    Helixi282 – 294Combined sources13
    Beta strandi300 – 303Combined sources4
    Helixi309 – 311Combined sources3
    Beta strandi312 – 315Combined sources4
    Helixi318 – 320Combined sources3
    Beta strandi322 – 324Combined sources3
    Beta strandi326 – 328Combined sources3
    Beta strandi335 – 338Combined sources4
    Helixi339 – 349Combined sources11
    Beta strandi352 – 354Combined sources3
    Helixi366 – 368Combined sources3
    Helixi372 – 386Combined sources15
    Helixi388 – 392Combined sources5
    Helixi395 – 430Combined sources36
    Beta strandi433 – 435Combined sources3
    Helixi437 – 455Combined sources19

    3D structure databases

    Select the link destinations:
    PDBei
    RCSB PDBi
    PDBji
    Links Updated
    PDB entryMethodResolution (Å)ChainPositionsPDBsum
    2CKOX-ray2.15A/B50-457[»]
    2CKPX-ray3.10A/B50-457[»]
    2CKQX-ray2.40A/B50-457[»]
    2I7QX-ray1.90A75-457[»]
    3F2RX-ray2.35A/B75-457[»]
    3G15X-ray1.70A/B75-457[»]
    3ZM9X-ray1.90A/B75-457[»]
    4BR3X-ray2.20A/B75-457[»]
    4CG8X-ray1.75A75-457[»]
    4CG9X-ray1.83A75-457[»]
    4CGAX-ray1.74A75-457[»]
    4DA5X-ray2.40A/B1-457[»]
    5AFVX-ray2.25A/B80-457[»]
    5EQEX-ray2.40A/B75-457[»]
    5EQPX-ray2.35A/B75-457[»]
    5EQYX-ray2.50A/B75-457[»]
    5FTGX-ray1.45A80-457[»]
    5FUTX-ray1.60A80-457[»]
    ProteinModelPortaliP35790.
    SMRiP35790.
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiP35790.

    Family & Domainsi

    Region

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Regioni119 – 121Substrate binding3

    Compositional bias

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Compositional biasi50 – 85Pro-richAdd BLAST36

    Sequence similaritiesi

    Belongs to the choline/ethanolamine kinase family.Curated

    Phylogenomic databases

    eggNOGiKOG2686. Eukaryota.
    COG0510. LUCA.
    GeneTreeiENSGT00530000062991.
    HOGENOMiHOG000041274.
    HOVERGENiHBG050943.
    InParanoidiP35790.
    KOiK14156.
    OMAiYMEYAQA.
    OrthoDBiEOG091G06UK.
    PhylomeDBiP35790.
    TreeFamiTF313549.

    Family and domain databases

    InterProiIPR011009. Kinase-like_dom.
    [Graphical view]
    SUPFAMiSSF56112. SSF56112. 1 hit.

    Sequences (2)i

    Sequence statusi: Complete.

    This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

    Isoform 1 (identifier: P35790-1) [UniParc]FASTAAdd to basket

    This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

    « Hide

            10         20         30         40         50
    MKTKFCTGGE AEPSPLGLLL SCGSGSAAPA PGVGQQRDAA SDLESKQLGG
    60 70 80 90 100
    QQPPLALPPP PPLPLPLPLP QPPPPQPPAD EQPEPRTRRR AYLWCKEFLP
    110 120 130 140 150
    GAWRGLREDE FHISVIRGGL SNMLFQCSLP DTTATLGDEP RKVLLRLYGA
    160 170 180 190 200
    ILQMRSCNKE GSEQAQKENE FQGAEAMVLE SVMFAILAER SLGPKLYGIF
    210 220 230 240 250
    PQGRLEQFIP SRRLDTEELS LPDISAEIAE KMATFHGMKM PFNKEPKWLF
    260 270 280 290 300
    GTMEKYLKEV LRIKFTEESR IKKLHKLLSY NLPLELENLR SLLESTPSPV
    310 320 330 340 350
    VFCHNDCQEG NILLLEGREN SEKQKLMLID FEYSSYNYRG FDIGNHFCEW
    360 370 380 390 400
    MYDYSYEKYP FFRANIRKYP TKKQQLHFIS SYLPAFQNDF ENLSTEEKSI
    410 420 430 440 450
    IKEEMLLEVN RFALASHFLW GLWSIVQAKI SSIEFGYMDY AQARFDAYFH

    QKRKLGV
    Length:457
    Mass (Da):52,249
    Last modified:April 14, 2009 - v3
    Checksum:i65F177ABE1AA3A12
    GO
    Isoform 2 (identifier: P35790-2) [UniParc]FASTAAdd to basket

    The sequence of this isoform differs from the canonical sequence as follows:
         155-172: Missing.

    Show »
    Length:439
    Mass (Da):50,155
    Checksum:i73507D716F15F9E4
    GO

    Experimental Info

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Sequence conflicti49 – 54GGQQPP → APTAA in BAA01547 (PubMed:1618328).Curated6
    Sequence conflicti87T → A in BAA01547 (PubMed:1618328).Curated1
    Sequence conflicti154M → V in BAA01547 (PubMed:1618328).Curated1

    Natural variant

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Natural variantiVAR_054863220S → G.1 PublicationCorresponds to variant rs17853641dbSNPEnsembl.1
    Natural variantiVAR_054864422L → Q.1 PublicationCorresponds to variant rs17853642dbSNPEnsembl.1

    Alternative sequence

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Alternative sequenceiVSP_009683155 – 172Missing in isoform 2. 1 PublicationAdd BLAST18

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    D10704 mRNA. Translation: BAA01547.1.
    AP002807 Genomic DNA. No translation available.
    AP002992 Genomic DNA. No translation available.
    BC036471 mRNA. Translation: AAH36471.1.
    CCDSiCCDS8178.1. [P35790-1]
    CCDS8179.1. [P35790-2]
    PIRiS23104.
    RefSeqiNP_001268.2. NM_001277.2. [P35790-1]
    NP_997634.1. NM_212469.1. [P35790-2]
    UniGeneiHs.77221.

    Genome annotation databases

    EnsembliENST00000265689; ENSP00000265689; ENSG00000110721. [P35790-1]
    ENST00000356135; ENSP00000348454; ENSG00000110721. [P35790-2]
    GeneIDi1119.
    KEGGihsa:1119.
    UCSCiuc001onj.4. human. [P35790-1]

    Keywords - Coding sequence diversityi

    Alternative splicing, Polymorphism

    Cross-referencesi

    Web resourcesi

    Atlas of Genetics and Cytogenetics in Oncology and Haematology

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    D10704 mRNA. Translation: BAA01547.1.
    AP002807 Genomic DNA. No translation available.
    AP002992 Genomic DNA. No translation available.
    BC036471 mRNA. Translation: AAH36471.1.
    CCDSiCCDS8178.1. [P35790-1]
    CCDS8179.1. [P35790-2]
    PIRiS23104.
    RefSeqiNP_001268.2. NM_001277.2. [P35790-1]
    NP_997634.1. NM_212469.1. [P35790-2]
    UniGeneiHs.77221.

    3D structure databases

    Select the link destinations:
    PDBei
    RCSB PDBi
    PDBji
    Links Updated
    PDB entryMethodResolution (Å)ChainPositionsPDBsum
    2CKOX-ray2.15A/B50-457[»]
    2CKPX-ray3.10A/B50-457[»]
    2CKQX-ray2.40A/B50-457[»]
    2I7QX-ray1.90A75-457[»]
    3F2RX-ray2.35A/B75-457[»]
    3G15X-ray1.70A/B75-457[»]
    3ZM9X-ray1.90A/B75-457[»]
    4BR3X-ray2.20A/B75-457[»]
    4CG8X-ray1.75A75-457[»]
    4CG9X-ray1.83A75-457[»]
    4CGAX-ray1.74A75-457[»]
    4DA5X-ray2.40A/B1-457[»]
    5AFVX-ray2.25A/B80-457[»]
    5EQEX-ray2.40A/B75-457[»]
    5EQPX-ray2.35A/B75-457[»]
    5EQYX-ray2.50A/B75-457[»]
    5FTGX-ray1.45A80-457[»]
    5FUTX-ray1.60A80-457[»]
    ProteinModelPortaliP35790.
    SMRiP35790.
    ModBaseiSearch...
    MobiDBiSearch...

    Protein-protein interaction databases

    BioGridi107543. 5 interactors.
    IntActiP35790. 1 interactor.
    STRINGi9606.ENSP00000265689.

    Chemistry databases

    BindingDBiP35790.
    ChEMBLiCHEMBL3117.
    DrugBankiDB00122. Choline.

    PTM databases

    iPTMnetiP35790.
    PhosphoSitePlusiP35790.

    Polymorphism and mutation databases

    BioMutaiCHKA.
    DMDMi226694197.

    Proteomic databases

    EPDiP35790.
    MaxQBiP35790.
    PaxDbiP35790.
    PeptideAtlasiP35790.
    PRIDEiP35790.

    Protocols and materials databases

    DNASUi1119.
    Structural Biology KnowledgebaseSearch...

    Genome annotation databases

    EnsembliENST00000265689; ENSP00000265689; ENSG00000110721. [P35790-1]
    ENST00000356135; ENSP00000348454; ENSG00000110721. [P35790-2]
    GeneIDi1119.
    KEGGihsa:1119.
    UCSCiuc001onj.4. human. [P35790-1]

    Organism-specific databases

    CTDi1119.
    DisGeNETi1119.
    GeneCardsiCHKA.
    H-InvDBHIX0017419.
    HGNCiHGNC:1937. CHKA.
    HPAiCAB072803.
    HPA024153.
    MIMi118491. gene.
    neXtProtiNX_P35790.
    OpenTargetsiENSG00000110721.
    PharmGKBiPA26468.
    GenAtlasiSearch...

    Phylogenomic databases

    eggNOGiKOG2686. Eukaryota.
    COG0510. LUCA.
    GeneTreeiENSGT00530000062991.
    HOGENOMiHOG000041274.
    HOVERGENiHBG050943.
    InParanoidiP35790.
    KOiK14156.
    OMAiYMEYAQA.
    OrthoDBiEOG091G06UK.
    PhylomeDBiP35790.
    TreeFamiTF313549.

    Enzyme and pathway databases

    UniPathwayiUPA00558; UER00741.
    UPA00753; UER00737.
    BioCyciMetaCyc:HS03334-MONOMER.
    ZFISH:HS03334-MONOMER.
    BRENDAi2.7.1.32. 2681.
    ReactomeiR-HSA-1483191. Synthesis of PC.
    R-HSA-1483213. Synthesis of PE.
    SABIO-RKP35790.

    Miscellaneous databases

    ChiTaRSiCHKA. human.
    EvolutionaryTraceiP35790.
    GeneWikiiCHKA.
    GenomeRNAii1119.
    PROiP35790.
    SOURCEiSearch...

    Gene expression databases

    BgeeiENSG00000110721.
    CleanExiHS_CHKA.
    ExpressionAtlasiP35790. baseline and differential.
    GenevisibleiP35790. HS.

    Family and domain databases

    InterProiIPR011009. Kinase-like_dom.
    [Graphical view]
    SUPFAMiSSF56112. SSF56112. 1 hit.
    ProtoNetiSearch...

    Entry informationi

    Entry nameiCHKA_HUMAN
    AccessioniPrimary (citable) accession number: P35790
    Secondary accession number(s): Q8NE29
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: June 1, 1994
    Last sequence update: April 14, 2009
    Last modified: November 30, 2016
    This is version 153 of the entry and version 3 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program
    DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

    Miscellaneousi

    Keywords - Technical termi

    3D-structure, Complete proteome, Reference proteome

    Documents

    1. Human chromosome 11
      Human chromosome 11: entries, gene names and cross-references to MIM
    2. Human entries with polymorphisms or disease mutations
      List of human entries with polymorphisms or disease mutations
    3. Human polymorphisms and disease mutations
      Index of human polymorphisms and disease mutations
    4. MIM cross-references
      Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
    5. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    6. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    7. SIMILARITY comments
      Index of protein domains and families

    Similar proteinsi

    Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
    100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
    90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
    50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.