ID   VA3_SOLIN               Reviewed;         234 AA.
AC   P35778; O16135; Q9TWZ2;
DT   01-JUN-1994, integrated into UniProtKB/Swiss-Prot.
DT   20-JUN-2001, sequence version 2.
DT   27-MAR-2024, entry version 113.
DE   RecName: Full=Venom allergen 3;
DE   AltName: Full=Allergen Sol i III {ECO:0000303|PubMed:8423273};
DE   AltName: Full=Antigen 5 {ECO:0000305};
DE            Short=Ag5 {ECO:0000305};
DE   AltName: Full=Cysteine-rich venom protein {ECO:0000305};
DE            Short=CRVP {ECO:0000305};
DE   AltName: Full=Venom allergen III;
DE   AltName: Allergen=Sol i 3 {ECO:0000303|PubMed:18761353};
DE   Flags: Precursor;
OS   Solenopsis invicta (Red imported fire ant) (Solenopsis wagneri).
OC   Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota;
OC   Neoptera; Endopterygota; Hymenoptera; Apocrita; Aculeata; Formicoidea;
OC   Formicidae; Myrmicinae; Solenopsis.
OX   NCBI_TaxID=13686;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA].
RC   TISSUE=Venom gland;
RA   Hoffman D.R., Farrar D., Schmidt M., McConnell T.J.;
RL   Submitted (JUL-1997) to the EMBL/GenBank/DDBJ databases.
RN   [2]
RP   NUCLEOTIDE SEQUENCE [MRNA] OF 23-234.
RC   TISSUE=Venom gland;
RX   PubMed=8588684; DOI=10.1111/j.1398-9995.1995.tb01196.x;
RA   Hoffman D.R.;
RT   "Fire ant venom allergy.";
RL   Allergy 50:535-544(1995).
RN   [3]
RP   PROTEIN SEQUENCE OF 23-234, AND SUBCELLULAR LOCATION.
RC   TISSUE=Venom;
RX   PubMed=8423273; DOI=10.1016/0091-6749(93)90298-t;
RA   Hoffman D.R.;
RT   "Allergens in Hymenoptera venom XXIV: the amino acid sequences of imported
RT   fire ant venom allergens Sol i II, Sol i III, and Sol i IV.";
RL   J. Allergy Clin. Immunol. 91:71-78(1993).
RN   [4]
RP   PROTEIN SEQUENCE OF 23-45, AND SUBCELLULAR LOCATION.
RC   TISSUE=Venom;
RX   PubMed=2355158; DOI=10.1016/0091-6749(90)90042-3;
RA   Hoffman D.R., Smith A.M., Schmidt M., Moffitt J.E., Guralnick M.;
RT   "Allergens in Hymenoptera venom. XXII. Comparison of venoms from two
RT   species of imported fire ants, Solenopsis invicta and richteri.";
RL   J. Allergy Clin. Immunol. 85:988-996(1990).
RN   [5]
RP   X-RAY CRYSTALLOGRAPHY (3.05 ANGSTROMS) OF 23-234, AND DISULFIDE BONDS.
RX   PubMed=18761353; DOI=10.1016/j.jmb.2008.08.023;
RA   Padavattan S., Schmidt M., Hoffman D.R., Markovic-Housley Z.;
RT   "Crystal structure of the major allergen from fire ant venom, Sol i 3.";
RL   J. Mol. Biol. 383:178-185(2008).
CC   -!- SUBCELLULAR LOCATION: Secreted {ECO:0000269|PubMed:2355158,
CC       ECO:0000269|PubMed:8423273}.
CC   -!- TISSUE SPECIFICITY: Expressed by the venom gland.
CC       {ECO:0000305|PubMed:2355158, ECO:0000305|PubMed:8423273}.
CC   -!- ALLERGEN: Causes an allergic reaction in human. The most common cause
CC       of insect venom allergy in the southeastern United States is the
CC       imported fire ant. {ECO:0000269|PubMed:8423273}.
CC   -!- SIMILARITY: Belongs to the CRISP family. {ECO:0000305}.
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DR   EMBL; AF012919; AAB65434.1; -; mRNA.
DR   PIR; C44582; B37330.
DR   RefSeq; NP_001291520.1; NM_001304591.1.
DR   PDB; 2VZN; X-ray; 3.05 A; A/B=23-234.
DR   PDBsum; 2VZN; -.
DR   AlphaFoldDB; P35778; -.
DR   SMR; P35778; -.
DR   Allergome; 3483; Sol i 3.0101.
DR   Allergome; 632; Sol i 3.
DR   EnsemblMetazoa; NM_001304591.1; NP_001291520.1; LOC105199703.
DR   GeneID; 105199703; -.
DR   KEGG; soc:105199703; -.
DR   HOGENOM; CLU_035730_7_2_1; -.
DR   OMA; YSCEIED; -.
DR   OrthoDB; 1028426at2759; -.
DR   EvolutionaryTrace; P35778; -.
DR   GO; GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
DR   CDD; cd05380; CAP_euk; 1.
DR   Gene3D; 3.40.33.10; CAP; 1.
DR   InterPro; IPR018244; Allrgn_V5/Tpx1_CS.
DR   InterPro; IPR014044; CAP_domain.
DR   InterPro; IPR035940; CAP_sf.
DR   InterPro; IPR001283; CRISP-related.
DR   InterPro; IPR034763; P14a_insect.
DR   InterPro; IPR002413; V5_allergen-like.
DR   PANTHER; PTHR10334; CYSTEINE-RICH SECRETORY PROTEIN-RELATED; 1.
DR   PANTHER; PTHR10334:SF582; SCP DOMAIN-CONTAINING PROTEIN; 1.
DR   Pfam; PF00188; CAP; 1.
DR   PIRSF; PIRSF038921; P14a; 1.
DR   PRINTS; PR00838; V5ALLERGEN.
DR   PRINTS; PR00837; V5TPXLIKE.
DR   SMART; SM00198; SCP; 1.
DR   SUPFAM; SSF55797; PR-1-like; 1.
DR   PROSITE; PS01009; CRISP_1; 1.
DR   PROSITE; PS01010; CRISP_2; 1.
PE   1: Evidence at protein level;
KW   3D-structure; Allergen; Direct protein sequencing; Disulfide bond;
KW   Secreted; Signal.
FT   SIGNAL          1..22
FT                   /evidence="ECO:0000269|PubMed:2355158,
FT                   ECO:0000269|PubMed:8423273"
FT   CHAIN           23..234
FT                   /note="Venom allergen 3"
FT                   /evidence="ECO:0000269|PubMed:8423273"
FT                   /id="PRO_0000006293"
FT   DOMAIN          69..218
FT                   /note="SCP"
FT   REGION          80..99
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   DISULFID        26..41
FT                   /evidence="ECO:0000269|PubMed:18761353"
FT   DISULFID        31..125
FT                   /evidence="ECO:0000269|PubMed:18761353"
FT   DISULFID        52..118
FT                   /evidence="ECO:0000269|PubMed:18761353"
FT   DISULFID        198..216
FT                   /evidence="ECO:0000269|PubMed:18761353"
FT   CONFLICT        183
FT                   /note="G -> E (in Ref. 3; AA sequence)"
FT                   /evidence="ECO:0000305"
FT   CONFLICT        194
FT                   /note="T -> S (in Ref. 3; AA sequence)"
FT                   /evidence="ECO:0000305"
FT   CONFLICT        199
FT                   /note="G -> A (in Ref. 3; AA sequence)"
FT                   /evidence="ECO:0000305"
FT   HELIX           29..33
FT                   /evidence="ECO:0007829|PDB:2VZN"
FT   HELIX           39..42
FT                   /evidence="ECO:0007829|PDB:2VZN"
FT   HELIX           62..80
FT                   /evidence="ECO:0007829|PDB:2VZN"
FT   HELIX           105..115
FT                   /evidence="ECO:0007829|PDB:2VZN"
FT   STRAND          129..131
FT                   /evidence="ECO:0007829|PDB:2VZN"
FT   STRAND          134..142
FT                   /evidence="ECO:0007829|PDB:2VZN"
FT   HELIX           150..158
FT                   /evidence="ECO:0007829|PDB:2VZN"
FT   HELIX           159..163
FT                   /evidence="ECO:0007829|PDB:2VZN"
FT   HELIX           166..168
FT                   /evidence="ECO:0007829|PDB:2VZN"
FT   STRAND          169..171
FT                   /evidence="ECO:0007829|PDB:2VZN"
FT   HELIX           176..188
FT                   /evidence="ECO:0007829|PDB:2VZN"
FT   STRAND          195..204
FT                   /evidence="ECO:0007829|PDB:2VZN"
FT   STRAND          210..220
FT                   /evidence="ECO:0007829|PDB:2VZN"
SQ   SEQUENCE   234 AA;  26351 MW;  539F510B59941D83 CRC64;
     MELIVSILWL AITAENLANT LATNYCNLQS CKRNNAIHTM CQYTSPTPGP MCLEYSNVGF
     TDAEKDAIVN KHNELRQRVA SGKEMRGTNG PQPPAVKMPN LTWDPELATI AQRWANQCTF
     EHDACRNVER FAVGQNIAAT SSSGKNKSTP NEMILLWYNE VKDFDNRWIS SFPSDDNILM
     KVGHYTQIVW AKTTKIGCGR IMFKEPDNWT KHYLVCNYGP AGNVLGAPIY EIKK
//