P35778 (VA3_SOLIN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names	Recommended name: Venom allergen 3 Alternative name(s): Allergen Sol i III Cysteine-rich venom protein Short name=CRVP Venom allergen III Allergen=Sol i 3
Organism	Solenopsis invicta (Red imported fire ant) (Solenopsis wagneri)
Taxonomic identifier	13686 [NCBI]
Taxonomic lineage	cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Protostomia › Ecdysozoa › Panarthropoda › Arthropoda › Mandibulata › Pancrustacea › Hexapoda › Insecta › Dicondylia › Pterygota › Neoptera › Endopterygota › Hymenoptera › Apocrita › Aculeata › Vespoidea › Formicidae › Myrmicinae › Solenopsidini › Solenopsis

Protein attributes

Sequence length	234 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Subcellular location	Secreted.
Tissue specificity	Expressed by the venom gland.
Allergenic properties	Causes an allergic reaction in human. The most common cause of insect venom allergy in the southeastern United States is the imported fire ant.
Sequence similarities	Belongs to the CRISP family. Contains 1 SCP domain.

Ontologies

Keywords
Cellular component	Secreted
Disease	Allergen
Domain	Signal
PTM	Disulfide bond
Technical term	3D-structure Direct protein sequencing
Gene Ontology (GO)
Cellular_component	extracellular region Inferred from electronic annotation. Source: UniProtKB-SubCell
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Signal peptide

1 – 22

22

Ref.3 Ref.4

Chain

23 – 234

212

Venom allergen 3

PRO_0000006293

Regions

Domain

69 – 218

150

SCP

Amino acid modifications

Disulfide bond

26 ↔ 41

Ref.5

Disulfide bond

31 ↔ 125

Ref.5

Disulfide bond

52 ↔ 118

Ref.5

Disulfide bond

198 ↔ 216

Ref.5

Experimental info

Sequence conflict

183

1

G → E AA sequence Ref.3

Sequence conflict

194

1

T → S AA sequence Ref.3

Sequence conflict

199

1

G → A AA sequence Ref.3

Secondary structure

1

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234

Helix Strand Turn

Details...

Helix

29 – 33

5

Helix

39 – 42

4

Helix

62 – 80

19

Helix

105 – 115

11

Beta strand

129 – 131

3

Beta strand

134 – 142

9

Helix

150 – 158

9

Helix

159 – 163

5

Helix

166 – 168

3

Beta strand

169 – 171

3

Helix

176 – 188

13

Beta strand

195 – 204

10

Beta strand

210 – 220

11

Sequences

	Sequence		Length	Mass (Da)
	P35778 [UniParc]. Last modified June 20, 2001. Version 2. Checksum: 539F510B59941D83	FASTA	234	26,351
	10 20 30 40 50 60 MELIVSILWL AITAENLANT LATNYCNLQS CKRNNAIHTM CQYTSPTPGP MCLEYSNVGF 70 80 90 100 110 120 TDAEKDAIVN KHNELRQRVA SGKEMRGTNG PQPPAVKMPN LTWDPELATI AQRWANQCTF 130 140 150 160 170 180 EHDACRNVER FAVGQNIAAT SSSGKNKSTP NEMILLWYNE VKDFDNRWIS SFPSDDNILM 190 200 210 220 230 KVGHYTQIVW AKTTKIGCGR IMFKEPDNWT KHYLVCNYGP AGNVLGAPIY EIKK

References

[1]	Hoffman D.R., Farrar D., Schmidt M., McConnell T.J. Submitted (JUL-1997) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Tissue: Venom gland.
[2]	"Fire ant venom allergy." Hoffman D.R. Allergy 50:535-544(1995) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 23-234. Tissue: Venom gland.
[3]	"Allergens in Hymenoptera venom XXIV: the amino acid sequences of imported fire ant venom allergens Sol i II, Sol i III, and Sol i IV." Hoffman D.R. J. Allergy Clin. Immunol. 91:71-78(1993) [PubMed] [Europe PMC] [Abstract] Cited for: PROTEIN SEQUENCE OF 23-234. Tissue: Venom.
[4]	"Allergens in Hymenoptera venom. XXII. Comparison of venoms from two species of imported fire ants, Solenopsis invicta and richteri." Hoffman D.R., Smith A.M., Schmidt M., Moffitt J.E., Guralnick M. J. Allergy Clin. Immunol. 85:988-996(1990) [PubMed] [Europe PMC] [Abstract] Cited for: PROTEIN SEQUENCE OF 23-45. Tissue: Venom.
[5]	"Crystal structure of the major allergen from fire ant venom, Sol i 3." Padavattan S., Schmidt M., Hoffman D.R., Markovic-Housley Z. J. Mol. Biol. 383:178-185(2008) [PubMed] [Europe PMC] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (3.05 ANGSTROMS) OF 23-234, DISULFIDE BONDS.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

AF012919 mRNA. Translation: AAB65434.1.

PIR

B37330. C44582.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
2VZN	X-ray	3.05	A/B	23-234	[»]

ProteinModelPortal

P35778.

ModBase

Search...

Protein family/group databases

Allergome

3483. Sol i 3.0101.
632. Sol i 3.

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Family and domain databases

Gene3D

3.40.33.10. 1 hit.

InterPro

IPR001283. Allrgn_V5/Tpx1.
IPR018244. Allrgn_V5/Tpx1_CS.
IPR014044. CAP_domain.
IPR002413. V5_allergen.
[Graphical view]

PANTHER

PTHR10334. PTHR10334. 1 hit.

Pfam

PF00188. CAP. 1 hit.
[Graphical view]

PRINTS

PR00838. V5ALLERGEN.
PR00837. V5TPXLIKE.

SMART

SM00198. SCP. 1 hit.
[Graphical view]

SUPFAM

SSF55797. SCP-like_extracellular. 1 hit.

PROSITE

PS01009. CRISP_1. 1 hit.
PS01010. CRISP_2. 1 hit.
[Graphical view]

ProtoNet

Search...

Other

EvolutionaryTrace

P35778.

Entry information

Entry name

VA3_SOLIN

Accession

Primary (citable) accession number: P35778
Secondary accession number(s): O16135, Q9TWZ2

Entry history

Integrated into UniProtKB/Swiss-Prot:	June 1, 1994
Last sequence update:	June 20, 2001
Last modified:	May 1, 2013
This is version 76 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)