P35775 (VA2_SOLIN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names	Recommended name: Venom allergen 2 Alternative name(s): Allergen Sol i II Venom allergen II Allergen=Sol i 2
Organism	Solenopsis invicta (Red imported fire ant) (Solenopsis wagneri)
Taxonomic identifier	13686 [NCBI]
Taxonomic lineage	cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Protostomia › Ecdysozoa › Panarthropoda › Arthropoda › Mandibulata › Pancrustacea › Hexapoda › Insecta › Dicondylia › Pterygota › Neoptera › Endopterygota › Hymenoptera › Apocrita › Aculeata › Vespoidea › Formicidae › Myrmicinae › Solenopsidini › Solenopsis

Protein attributes

Sequence length	138 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Subunit structure	Homodimer; disulfide-linked. Ref.4
Subcellular location	Secreted.
Tissue specificity	Expressed by the venom gland.
Allergenic properties	Causes an allergic reaction in human. The most common cause of insect venom allergy in the southeastern United States is the imported fire ant.
Sequence similarities	Belongs to the ant venom allergen 2/4 family.

Ontologies

Keywords
Cellular component	Secreted
Disease	Allergen
Domain	Signal
PTM	Disulfide bond
Technical term	3D-structure Direct protein sequencing
Gene Ontology (GO)
Cellular_component	extracellular region Inferred from electronic annotation. Source: UniProtKB-SubCell
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Signal peptide

1 – 19

19

Ref.2 Ref.3

Chain

20 – 138

119

Venom allergen 2

PRO_0000022646

Amino acid modifications

Disulfide bond

34 ↔ 57

Ref.4

Disulfide bond

41

Interchain Ref.4

Disulfide bond

81 ↔ 94

Ref.4

Disulfide bond

101 ↔ 122

Ref.4

Secondary structure

1

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

138

Helix Strand Turn

Details...

Helix

21 – 37

17

Helix

48 – 50

3

Helix

52 – 61

10

Turn

62 – 65

4

Helix

70 – 84

15

Helix

88 – 105

18

Beta strand

108 – 110

3

Helix

112 – 125

14

Helix

128 – 131

4

Sequences

	Sequence		Length	Mass (Da)
	P35775 [UniParc]. Last modified June 1, 1994. Version 1. Checksum: DA30E1E41041EE75	FASTA	138	15,289
	10 20 30 40 50 60 MKSFVLATCL LGFAQIIYAD NKELKIIRKD VAECLRTLPK CGNQPDDPLA RVDVWHCAMA 70 80 90 100 110 120 KRGVYDNPDP AVIKERSMKM CTKIITDPAN VENCKKVASR CVDRETQGPK SNRQKAVNII 130 GCALRAGVAE TTVLARKK

References

[1]	"Nucleotide sequence of cDNA encoding the fire ant venom protein Sol i II." Schmidt M., Walker R.B., Hoffman D.R., McConnell T.J. FEBS Lett. 319:138-140(1993) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Tissue: Venom gland.
[2]	"Allergens in Hymenoptera venom XXIV: the amino acid sequences of imported fire ant venom allergens Sol i II, Sol i III, and Sol i IV." Hoffman D.R. J. Allergy Clin. Immunol. 91:71-78(1993) [PubMed] [Europe PMC] [Abstract] Cited for: PROTEIN SEQUENCE OF 20-138. Tissue: Venom.
[3]	"Allergens in Hymenoptera venom. XXII. Comparison of venoms from two species of imported fire ants, Solenopsis invicta and richteri." Hoffman D.R., Smith A.M., Schmidt M., Moffitt J.E., Guralnick M. J. Allergy Clin. Immunol. 85:988-996(1990) [PubMed] [Europe PMC] [Abstract] Cited for: PROTEIN SEQUENCE OF 20-87. Tissue: Venom.
[4]	"Crystal structure of Sol I 2: a major allergen from fire ant venom." Borer A.S., Wassmann P., Schmidt M., Hoffman D.R., Zhou J.J., Wright C., Schirmer T., Markovic-Housley Z. J. Mol. Biol. 415:635-648(2012) [PubMed] [Europe PMC] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (2.6 ANGSTROMS) OF 20-138, SUBUNIT, DISULFIDE BONDS.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

L09560 mRNA. No translation available.

PIR

A37330. S32389.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
2YGU	X-ray	2.60	A/B/C/D/E/F/G/H	20-138	[»]

ModBase

Search...

Protein family/group databases

Allergome

3482. Sol i 2.0101.
631. Sol i 2.

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Family and domain databases

InterPro

IPR020181. Ant_venom_allergen_Sol_i_2/4.
[Graphical view]

ProDom

PD031252. Ant_venom_allergen_Sol_i_2/4. 1 hit.
[Graphical view] [Entries sharing at least one domain]

ProtoNet

Search...

Entry information

Entry name

VA2_SOLIN

Accession

Primary (citable) accession number: P35775

Entry history

Integrated into UniProtKB/Swiss-Prot:	June 1, 1994
Last sequence update:	June 1, 1994
Last modified:	April 3, 2013
This is version 62 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)